RLG00000025587

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000824
Physical Location & Seq
Reverse (-)
46940249 .. 46944445
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025587

Sequence Viewer

Length: 765 bp
ATGTCGACTGACCCCGATTTCATCTTCGCCTTCAACGATAATAACTTCTCTGACCGGACAGCTCCGGATCAAGATCATGGTCGGCTCTTCGAGGAAGCTAAGATCCTTGTTGTCCTCAACATCTCCTTCGTCTTCTCCTTCTCGAGCCTCAGAGCTAAAGGAAGAACAAGGCCGCTGAATCCATCGTTGATCAGAATAAACTCGTCCCCGGCCATGATTTGGCAGAGACAGCGTTTGCCTGCTATGGAGAAGGCGGCGGAGGAGCGAGTTCGATCTCCAGGAGGCCCTGACGATGTCGTTTTAGTCGATTTCTGGGAGGACCTATTCCTTCTTGCTCCAACTCCACGTTTTACCCTTAACAGTACTGGCACTTGCAAGCAGAAGAAGAACAAGGCCAAGGCATGCCAAGTCGCTCCGGCTGGGTGTTTCACAGTCTACGTTGGACCCGAAAAGCAGCTATTTGTGGTGAGGATGGAGTTTCTTAATCATCCATTGTTCAGGATGCCGTTGGAGGATGCAGCATTGGAGTATGGGTACAAAAGTGACGGCCCGATATTGCTTCCTTGCCATGTGGATCTGTTCTGCAATGTTATGGCAGAGATTGAGAGCAATGATATCAATGAGGATAGGATCACTGCTCCCAGTAATTGTTCTCCGATAAACTTCAGTCCAGCTCGTCGTAGAAATTGTGCCAGCAACAGAGGATACGGTGGTGCTTATAGGGTACTTACTCCAACATCGTCATCATCAATGGTGGTTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

255

Amino Acids

28.41

Weight (kDa)

7.57

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 126 - 199 1e-19 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 219
AccI GTMKAC 2 cut(s) 5, 435
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 3 cut(s) 173, 254, 257
AclWI GGATC 4 cut(s) 75, 97, 582, 638
AcoI YGGCCR 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 3 cut(s) 364, 536, 726
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 1 cut(s) 34
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 5 cut(s) 62, 98, 155, 457, 674
AluI AGCT 5 cut(s) 62, 98, 155, 457, 674
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 4 cut(s) 75, 97, 582, 638
Ama87I CYCGRG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 64
AoxI GGCC 5 cut(s) 170, 210, 283, 393, 547
ApeKI GCWGC 2 cut(s) 454, 518
AspS9I GGNCC 4 cut(s) 284, 319, 443, 548
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 478
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 2 cut(s) 319, 443
BaeI ACNNNNGTAYC 4 cut(s) 526, 559, 697, 730
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 466, 530
BccI CCATC 2 cut(s) 190, 466
BceAI ACGGC 2 cut(s) 490, 562
BciT130I CCWGG 1 cut(s) 279
BciVI GTATCC 1 cut(s) 698
BclI TGATCA 1 cut(s) 189
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 763
BfuI GTATCC 1 cut(s) 698
BisI GCNGC 4 cut(s) 173, 255, 455, 519
BlsI GCNGC 4 cut(s) 174, 256, 456, 520
BmcAI AGTACT 1 cut(s) 364
Bme1390I CCNGG 2 cut(s) 209, 279
Bme18I GGWCC 2 cut(s) 319, 443
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 4 cut(s) 284, 319, 443, 548
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 2 cut(s) 209, 279
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 2 cut(s) 492, 505
BmuI ACTGGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 207, 396
BsaWI WCCGGW 2 cut(s) 54, 64
BsaXI ACNNNNNCTCC 2 cut(s) 518, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 2 cut(s) 370, 642
Bse3DI GCAATG 2 cut(s) 592, 616
Bse8I GATNNNNATC 1 cut(s) 72
BseAI TCCGGA 1 cut(s) 64
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 207, 396
BseGI GGATG 4 cut(s) 477, 487, 507, 520
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMI GCAATG 2 cut(s) 592, 616
BseMII CTCAG 1 cut(s) 163
BseNI ACTGG 2 cut(s) 370, 642
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 466, 530
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 5 cut(s) 172, 212, 285, 395, 549
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 4 cut(s) 55, 65, 209, 416
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 5 cut(s) 172, 212, 285, 395, 549
BsoBI CYCGRG 1 cut(s) 142
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
BspACI CCGC 3 cut(s) 173, 254, 257
BspANI GGCC 5 cut(s) 172, 212, 285, 395, 549
BspCNI CTCAG 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 445
BspPI GGATC 4 cut(s) 75, 97, 582, 638
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 592, 616
BsrI ACTGG 2 cut(s) 370, 642
BssECI CCNNGG 2 cut(s) 207, 396
BssMI GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 362, 433, 710
Bst6I CTCTTC 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 240, 377, 403, 694
BstDEI CTNAG 2 cut(s) 99, 149
BstEII GGTNACC 1 cut(s) 757
BstF5I GGATG 4 cut(s) 477, 487, 507, 520
BstKTI GATC 7 cut(s) 70, 76, 105, 192, 275, 577, 633
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 1 cut(s) 405
BstPI GGTNACC 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 207, 277
BstV1I GCAGC 2 cut(s) 466, 530
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 2 cut(s) 102, 574
BstYI RGATCY 2 cut(s) 102, 574
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 5 cut(s) 172, 212, 285, 395, 549
BtsCI GGATG 4 cut(s) 477, 487, 507, 520
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 1 cut(s) 633
Cac8I GCNNGC 4 cut(s) 240, 377, 403, 694
Cfr13I GGNCC 4 cut(s) 284, 319, 443, 548
Csp6I GTAC 3 cut(s) 363, 535, 725
CviAII CATG 4 cut(s) 77, 214, 402, 569
CviQI GTAC 3 cut(s) 363, 535, 725
DdeI CTNAG 2 cut(s) 99, 149
DpnI GATC 7 cut(s) 69, 75, 104, 191, 274, 576, 632
DpnII GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 396
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 2 cut(s) 319, 443
Eco57I CTGAAG 1 cut(s) 649
Eco88I CYCGRG 1 cut(s) 142
Eco91I GGTNACC 1 cut(s) 757
EcoO109I RGGNCCY 2 cut(s) 284, 319
EcoO65I GGTNACC 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 277
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 396
FaeI CATG 4 cut(s) 80, 217, 405, 572
FaiI YATR 8 cut(s) 78, 215, 245, 403, 531, 570, 593, 720
FaqI GGGAC 1 cut(s) 190
FatI CATG 4 cut(s) 76, 213, 401, 568
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 2 cut(s) 5, 435
Fnu4HI GCNGC 4 cut(s) 173, 255, 455, 519
FokI GGATG 4 cut(s) 474, 484, 514, 527
Fsp4HI GCNGC 4 cut(s) 173, 255, 455, 519
FspBI CTAG 1 cut(s) 763
GluI GCNGC 4 cut(s) 173, 255, 455, 519
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 5 cut(s) 172, 212, 285, 395, 549
HapII CCGG 4 cut(s) 55, 65, 209, 416
Hin1II CATG 4 cut(s) 80, 217, 405, 572
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 178
HpaII CCGG 4 cut(s) 55, 65, 209, 416
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 2 cut(s) 6, 436
Hpy188I TCNGA 4 cut(s) 52, 152, 194, 657
Hpy188III TCNNGA 4 cut(s) 65, 71, 142, 499
Hpy8I GTNNAC 2 cut(s) 6, 436
Hpy99I CGWCG 1 cut(s) 681
HpyAV CCTTC 5 cut(s) 40, 136, 148, 244, 338
HpyCH4III ACNGT 3 cut(s) 362, 433, 710
HpyCH4IV ACGT 2 cut(s) 346, 438
HpyCH4V TGCA 3 cut(s) 375, 518, 585
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 2 cut(s) 99, 149
HpySE526I ACGT 2 cut(s) 346, 438
Hsp92II CATG 4 cut(s) 80, 217, 405, 572
Kpn2I TCCGGA 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 5 cut(s) 67, 262, 340, 418, 643
Lsp1109I GCAGC 2 cut(s) 466, 530
LweI GCATC 2 cut(s) 492, 505
MaeI CTAG 1 cut(s) 763
MaeII ACGT 2 cut(s) 346, 438
MaeIII GTNAC 2 cut(s) 542, 757
MalI GATC 7 cut(s) 69, 75, 104, 191, 274, 576, 632
MboI GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
MboII GAAGA 6 cut(s) 16, 79, 124, 174, 394, 397
MflI RGATCY 2 cut(s) 102, 574
MluCI AATT 2 cut(s) 646, 685
MmeI TCCRAC 4 cut(s) 362, 421, 489, 758
MroI TCCGGA 1 cut(s) 64
MseI TTAA 2 cut(s) 357, 483
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 4 cut(s) 55, 65, 209, 416
MspR9I CCNGG 2 cut(s) 209, 279
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 1 cut(s) 229
NciI CCSGG 1 cut(s) 209
NdeII GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
NlaIII CATG 4 cut(s) 80, 217, 405, 572
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 542
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PaeR7I CTCGAG 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 3 cut(s) 33, 303, 444
PfeI GAWTC 1 cut(s) 178
PflFI GACNNNGTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 219
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 4 cut(s) 174, 256, 456, 520
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 277
PspEI GGTNACC 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 4 cut(s) 284, 319, 443, 548
PspPPI RGGWCCY 1 cut(s) 319
PsuI RGATCY 2 cut(s) 102, 574
PsyI GACNNNGTC 1 cut(s) 293
RsaI GTAC 3 cut(s) 364, 536, 726
RsaNI GTAC 3 cut(s) 363, 535, 725
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 2 cut(s) 357, 483
SatI GCNGC 4 cut(s) 173, 255, 455, 519
Sau3AI GATC 7 cut(s) 67, 73, 102, 189, 272, 574, 630
Sau96I GGNCC 4 cut(s) 284, 319, 443, 548
ScaI AGTACT 1 cut(s) 364
ScrFI CCNGG 2 cut(s) 209, 279
SetI ASST 9 cut(s) 64, 100, 157, 324, 349, 441, 459, 676, 764
SfaNI GCATC 2 cut(s) 492, 505
Sfr274I CTCGAG 1 cut(s) 142
SinI GGWCC 2 cut(s) 319, 443
SlaI CTCGAG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
SphI GCATGC 1 cut(s) 405
Sse9I AATT 2 cut(s) 646, 685
SsiI CCGC 3 cut(s) 173, 254, 257
SspMI CTAG 1 cut(s) 763
StyD4I CCNGG 2 cut(s) 207, 277
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 3 cut(s) 362, 433, 710
TaiI ACGT 2 cut(s) 349, 441
TaqI TCGA 5 cut(s) 5, 90, 143, 271, 306
TasI AATT 2 cut(s) 646, 685
TatI WGTACW 1 cut(s) 362
TauI GCSGC 2 cut(s) 175, 257
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 357, 483
Tru9I TTAA 2 cut(s) 357, 483
TscAI CASTG 1 cut(s) 640
TseFI GTSAC 1 cut(s) 542
TseI GCWGC 2 cut(s) 454, 518
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 1 cut(s) 10
TspRI CASTG 1 cut(s) 640
Tth111I GACNNNGTC 1 cut(s) 293
Van91I CCANNNNNTGG 1 cut(s) 219
VpaK11BI GGWCC 2 cut(s) 319, 443
XceI RCATGY 1 cut(s) 405
XhoI CTCGAG 1 cut(s) 142
XmiI GTMKAC 2 cut(s) 5, 435
XspI CTAG 1 cut(s) 763
ZrmI AGTACT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.