RchiOBHm_Chr7g0189471

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000824
Physical Location & Seq
Forward (+)
8796426 .. 8796983
558 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16921

Sequence Viewer

Length: 558 bp
ATGGATATCGTGAAGGGAAAGTGGACGAAGAATCTGATCATCAAGGCGTGGAAGCGATGCAGTACTTTACCGAAACAAAGCAGCAGGAAAGGATCTACAGCAGCCGCTTCATTGTCAAGGAGCAAATCATGGAGCTCCAGTACTACTAGTAGTACTGGCACTTGCAAGCAGAAGAAGAACAAGGCCAAGACATGTCAAGTCGCTCCAGTTGGGTGTTTCACGGTCTATGTTGGACCCGAAAAGCAGCGATTTGTGGTGAGGATGGAGTATGTTAATCATCCATTGTTCAAGATGCTGCTGGAGGATGCAGCATTGGAGTATGGGTACAAAAATGACGGTCCGATATTGCTCCCTTGCCATGTGGATCTGTTCTGCAATGTTATGGCAGAGATGGAGAGCAATGATATCGATGAGGATAGGATCAGTGCTCCCAGTAATTGTTCTCCGATAATCTTCAGTCCAGCTCGTCGTAGAAATTGTGCCAGTAACAGAGGTTACGGTGGTGCTTATAGGGTACTTACTCCAACATCATCGTCATCATCAATGGTGGTTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

185

Amino Acids

20.4

Weight (kDa)

9.55

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 43 - 129 2e-24 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 105
AclWI GGATC 3 cut(s) 100, 372, 428
AcuI CTGAAG 1 cut(s) 439
AfaI GTAC 5 cut(s) 64, 142, 154, 326, 516
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 1 cut(s) 289
AhlI ACTAGT 1 cut(s) 146
AluBI AGCT 2 cut(s) 135, 464
AluI AGCT 2 cut(s) 135, 464
Alw21I GWGCWC 2 cut(s) 137, 430
AlwI GGATC 3 cut(s) 100, 372, 428
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 5 cut(s) 81, 101, 244, 295, 308
AspS9I GGNCC 2 cut(s) 233, 338
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 2 cut(s) 233, 338
BanII GRGCYC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 137, 430
BbvI GCAGC 5 cut(s) 93, 113, 256, 282, 320
BccI CCATC 2 cut(s) 256, 385
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BclI TGATCA 1 cut(s) 36
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 2 cut(s) 147, 556
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
BlsI GCNGC 6 cut(s) 83, 103, 106, 246, 297, 310
BmcAI AGTACT 3 cut(s) 64, 142, 154
Bme18I GGWCC 2 cut(s) 233, 338
BmgT120I GGNCC 2 cut(s) 233, 338
BmiI GGNNCC 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 3 cut(s) 47, 282, 295
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 3 cut(s) 121, 189, 320
Bsa29I ATCGAT 1 cut(s) 408
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 308, 338
Bse1I ACTGG 5 cut(s) 138, 160, 206, 432, 483
Bse3DI GCAATG 2 cut(s) 382, 406
BseCI ATCGAT 1 cut(s) 408
BseGI GGATG 3 cut(s) 267, 277, 310
BseMI GCAATG 2 cut(s) 382, 406
BseNI ACTGG 5 cut(s) 138, 160, 206, 432, 483
BseXI GCAGC 5 cut(s) 93, 113, 256, 282, 320
BshFI GGCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 137, 430
BsnI GGCC 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 137, 430
Bsp143I GATC 4 cut(s) 36, 92, 364, 420
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 185
BspDI ATCGAT 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 3 cut(s) 100, 372, 428
BsrDI GCAATG 2 cut(s) 382, 406
BsrI ACTGG 5 cut(s) 138, 160, 206, 432, 483
BssMI GATC 4 cut(s) 36, 92, 364, 420
Bst4CI ACNGT 3 cut(s) 223, 338, 500
BstC8I GCNNGC 1 cut(s) 167
BstEII GGTNACC 1 cut(s) 550
BstF5I GGATG 3 cut(s) 267, 277, 310
BstKTI GATC 4 cut(s) 39, 95, 367, 423
BstMBI GATC 4 cut(s) 36, 92, 364, 420
BstNSI RCATGY 1 cut(s) 195
BstPI GGTNACC 1 cut(s) 550
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 5 cut(s) 93, 113, 256, 282, 320
BstX2I RGATCY 2 cut(s) 92, 364
BstYI RGATCY 2 cut(s) 92, 364
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 1 cut(s) 185
BsuTUI ATCGAT 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 3 cut(s) 267, 277, 310
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 233, 338
ClaI ATCGAT 1 cut(s) 408
CpoI CGGWCCG 1 cut(s) 338
Csp6I GTAC 5 cut(s) 63, 141, 153, 325, 515
CspI CGGWCCG 1 cut(s) 338
CviAII CATG 3 cut(s) 129, 192, 359
CviJI RGCY 4 cut(s) 104, 135, 185, 464
CviKI_1 RGCY 4 cut(s) 104, 135, 185, 464
CviQI GTAC 5 cut(s) 63, 141, 153, 325, 515
DpnI GATC 4 cut(s) 38, 94, 366, 422
DpnII GATC 4 cut(s) 36, 92, 364, 420
Ecl136II GAGCTC 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 137
Eco32I GATATC 2 cut(s) 7, 406
Eco47I GGWCC 2 cut(s) 233, 338
Eco53kI GAGCTC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 439
Eco91I GGTNACC 1 cut(s) 550
EcoICRI GAGCTC 1 cut(s) 135
EcoO65I GGTNACC 1 cut(s) 550
EcoRV GATATC 2 cut(s) 7, 406
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 3 cut(s) 132, 195, 362
FaiI YATR 8 cut(s) 130, 193, 228, 270, 321, 360, 383, 510
FatI CATG 3 cut(s) 128, 191, 358
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
FokI GGATG 3 cut(s) 264, 274, 317
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
FspBI CTAG 2 cut(s) 147, 556
GluI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
GsuI CTGGAG 3 cut(s) 121, 189, 320
HaeIII GGCC 1 cut(s) 185
Hin1II CATG 3 cut(s) 132, 195, 362
HinfI GANTC 1 cut(s) 31
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 36, 342, 447
Hpy188III TCNNGA 2 cut(s) 10, 289
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 3 cut(s) 223, 338, 500
HpyCH4V TGCA 4 cut(s) 60, 165, 308, 375
Hsp92II CATG 3 cut(s) 132, 195, 362
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 4 cut(s) 36, 92, 364, 420
LmnI GCTCC 6 cut(s) 120, 132, 140, 208, 354, 433
LpnPI CCDG 8 cut(s) 70, 141, 151, 219, 284, 445, 474, 496
Lsp1109I GCAGC 5 cut(s) 93, 113, 256, 282, 320
LweI GCATC 3 cut(s) 47, 282, 295
MaeI CTAG 2 cut(s) 147, 556
MaeIII GTNAC 3 cut(s) 485, 494, 550
MalI GATC 4 cut(s) 38, 94, 366, 422
MboI GATC 4 cut(s) 36, 92, 364, 420
MboII GAAGA 4 cut(s) 40, 184, 187, 445
MflI RGATCY 2 cut(s) 92, 364
MhlI GDGCHC 2 cut(s) 137, 430
MluCI AATT 2 cut(s) 436, 475
MmeI TCCRAC 2 cut(s) 211, 548
MnlI CCTC 4 cut(s) 252, 295, 406, 485
MseI TTAA 1 cut(s) 273
NdeII GATC 4 cut(s) 36, 92, 364, 420
NlaIII CATG 3 cut(s) 132, 195, 362
NlaIV GGNNCC 1 cut(s) 235
NspI RCATGY 1 cut(s) 195
PciI ACATGT 1 cut(s) 191
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 6 cut(s) 83, 103, 106, 246, 297, 310
PscI ACATGT 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 550
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 2 cut(s) 233, 338
PsuI RGATCY 2 cut(s) 92, 364
RsaI GTAC 5 cut(s) 64, 142, 154, 326, 516
RsaNI GTAC 5 cut(s) 63, 141, 153, 325, 515
Rsr2I CGGWCCG 1 cut(s) 338
RsrII CGGWCCG 1 cut(s) 338
SacI GAGCTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 6 cut(s) 82, 102, 105, 245, 296, 309
Sau3AI GATC 4 cut(s) 36, 92, 364, 420
Sau96I GGNCC 2 cut(s) 233, 338
ScaI AGTACT 3 cut(s) 64, 142, 154
SduI GDGCHC 2 cut(s) 137, 430
SetI ASST 4 cut(s) 137, 466, 496, 557
SfaNI GCATC 3 cut(s) 47, 282, 295
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 2 cut(s) 233, 338
SpeI ACTAGT 1 cut(s) 146
Sse9I AATT 2 cut(s) 436, 475
SsiI CCGC 1 cut(s) 105
SspMI CTAG 2 cut(s) 147, 556
SstI GAGCTC 1 cut(s) 137
TaaI ACNGT 3 cut(s) 223, 338, 500
TaqI TCGA 1 cut(s) 408
TasI AATT 2 cut(s) 436, 475
TatI WGTACW 3 cut(s) 62, 140, 152
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 430
TseI GCWGC 5 cut(s) 81, 101, 244, 295, 308
TspDTI ATGAA 1 cut(s) 99
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 233, 338
XceI RCATGY 1 cut(s) 195
XspI CTAG 2 cut(s) 147, 556
ZrmI AGTACT 3 cut(s) 64, 142, 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.