MD06G1162800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001697
Physical Location & Seq
Reverse (-)
30327768 .. 30328088
321 bp
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UTR
Exon/CDS
Intron
MD06G1162800.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGATTCTCAAGTCCTCATACTCCTTGTTTTCCTCGTACCCTTTATTTTCTTGATCTTGATCAAACATAGAAAGAAAAGTATTGCTTCTCAAGCAAGGAGGCGGCCTCTCATTGGCAACCTTCACAAGCTCTCCGATGGCGGCTTACCCCATCAAGTCCTTGAGCGCCTAGCCGCCAAATATGGAGATCTCATGTTTTTACAATTAGGCTCGGTATCCACTCTAGTGGTCTTCTCATCTCATATGGCAAGAGAGATCTTCAAAACTCATGACCTTATTTTTTCAGGTCGGCTGACCTTATATATTCCAAAGAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

107

Amino Acids

12.07

Weight (kDa)

10.54

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 97 4e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 103, 141, 174
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 113
AgsI TTSAA 1 cut(s) 262
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 2 cut(s) 130, 316
AluI AGCT 2 cut(s) 130, 316
AoxI GGCC 1 cut(s) 104
AspLEI GCGC 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 223
BccI CCATC 2 cut(s) 131, 159
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 1 cut(s) 60
BfaI CTAG 2 cut(s) 170, 224
BfoI RGCGCY 1 cut(s) 169
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 2 cut(s) 187, 255
BisI GCNGC 3 cut(s) 104, 142, 174
BlsI GCNGC 3 cut(s) 105, 143, 175
BpiI GAAGAC 1 cut(s) 223
BplI GAGNNNNNCTC 2 cut(s) 91, 123
BpuEI CTTGAG 2 cut(s) 75, 182
BsaBI GATNNNNATC 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse8I GATNNNNATC 1 cut(s) 59
BseJI GATNNNNATC 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 113
BshFI GGCC 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 113
BsnI GGCC 1 cut(s) 106
Bsp143I GATC 4 cut(s) 54, 60, 187, 255
BspACI CCGC 3 cut(s) 103, 141, 174
BspANI GGCC 1 cut(s) 106
BspHI TCATGA 1 cut(s) 268
BssMI GATC 4 cut(s) 54, 60, 187, 255
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 4 cut(s) 57, 63, 190, 258
BstMBI GATC 4 cut(s) 54, 60, 187, 255
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 187, 255
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 2 cut(s) 187, 255
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 1 cut(s) 106
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 38
CviAII CATG 2 cut(s) 193, 269
CviJI RGCY 7 cut(s) 106, 130, 144, 173, 210, 292, 316
CviKI_1 RGCY 7 cut(s) 106, 130, 144, 173, 210, 292, 316
CviQI GTAC 1 cut(s) 38
DpnI GATC 4 cut(s) 56, 62, 189, 257
DpnII GATC 4 cut(s) 54, 60, 187, 255
FaeI CATG 2 cut(s) 196, 272
FaiI YATR 9 cut(s) 20, 69, 183, 194, 243, 245, 270, 301, 303
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FatI CATG 2 cut(s) 192, 268
FauNDI CATATG 1 cut(s) 243
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 104, 142, 174
Fsp4HI GCNGC 3 cut(s) 104, 142, 174
FspBI CTAG 2 cut(s) 170, 224
GlaI GCGC 1 cut(s) 167
GluI GCNGC 3 cut(s) 104, 142, 174
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 1 cut(s) 106
HhaI GCGC 1 cut(s) 168
Hin1II CATG 2 cut(s) 196, 272
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 1 cut(s) 5
Hpy188I TCNGA 1 cut(s) 136
Hpy188III TCNNGA 3 cut(s) 52, 58, 269
HpyAV CCTTC 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
Hsp92II CATG 2 cut(s) 196, 272
HspAI GCGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 4 cut(s) 54, 60, 187, 255
LpnPI CCDG 1 cut(s) 270
MaeI CTAG 2 cut(s) 170, 224
MalI GATC 4 cut(s) 56, 62, 189, 257
MboI GATC 4 cut(s) 54, 60, 187, 255
MboII GAAGA 2 cut(s) 223, 250
MflI RGATCY 2 cut(s) 187, 255
MluCI AATT 1 cut(s) 203
MnlI CCTC 4 cut(s) 26, 44, 93, 117
MslI CAYNNNNRTG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeI CATATG 1 cut(s) 243
NdeII GATC 4 cut(s) 54, 60, 187, 255
NlaIII CATG 2 cut(s) 196, 272
OliI CACNNNNGTG 1 cut(s) 224
PagI TCATGA 1 cut(s) 268
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 3 cut(s) 105, 143, 175
PsuI RGATCY 2 cut(s) 187, 255
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
RseI CAYNNNNRTG 1 cut(s) 224
SatI GCNGC 3 cut(s) 104, 142, 174
Sau3AI GATC 4 cut(s) 54, 60, 187, 255
SetI ASST 6 cut(s) 123, 132, 276, 289, 299, 318
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 3 cut(s) 9, 90, 161
SmoI CTYRAG 3 cut(s) 9, 90, 161
Sse9I AATT 1 cut(s) 203
SsiI CCGC 3 cut(s) 103, 141, 174
SspMI CTAG 2 cut(s) 170, 224
TasI AATT 1 cut(s) 203
TauI GCSGC 3 cut(s) 106, 144, 176
TfiI GAWTC 1 cut(s) 5
XspI CTAG 2 cut(s) 170, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.