Rh7AG123400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001697
Physical Location & Seq
Reverse (-)
9689522 .. 9692958
3437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG123400.1

Sequence Viewer

Length: 1290 bp
ATGGTGCTCCTACTCTTTATTTTTCTTATACCCTTTACTTTCTTGCTCTTGATGAAGCGAAGAAAGCAGAATGTCCAAGCAAAGAGATTGCCTCCTGGTCCTAGAAGGCTACCTGTAATTGGGAACCTTCACCATCTTTCTGATGATTTACCTCATTGTGCCTTTGAACACCTTTCCAGTCATTACGGACCGATCATGTTCTTGCAATTAGGCTCAAGATCTACTTTAGTAGTCTCTTCAGCCGAAATGACTAGAGAGGTTTTCAAAACTCATGACCTCATTTTTTCAGGTAGACCAGATTTCTTGTATGTTGCAAAGAGGCTTAGCTATGACTCCAGTGTATCATTTTCTCCCATTGGTAAGTATTGGAGGGAGGTTAGAAAGATTGTGATCTTGGAACTACTTAGTGCAAAGAGGATTCAAATGTTTCGTTCTGTGAGAGACGAAGAGGTTGGACATATGATTGATGACATAGCTCATACTTCTAATAGTCTCATCAATCTCAATGCACTGACACATTTCTTATCCAACAGTGTCTTGTGCCGCTCGGCTTTTGGCAAAAAGTATGATGGTGCTGGAGGTGTTATTAGCAAGAACAGAATTTTTGAATTGCTTGAAGAGGCAATGAACTTGTTGGGAGGGTTCTGCATATCGGATTTTCTGCCATGGATGGGTTGGCTAAACAAGTTCAATGGTCTTGAAAAAAGGGTAGACAAGTGTTTTAAAGGGTTGGACAGTTTATATGACAAAGTGATTGAGGAACACCTTGACCCAAAAAGGCCTACTCCGGAGCATGAAGATCTTGTCGATGTGCTACTTCGAGTTCAGAAAGATCCAAGTCAAGCAATAGCACTTACTAACGACCAAATCAAGGGTGTCATCACTAAAAATGCTAGAGGAAAGTGGTTGCCTTATGGTCCTAGAAGGCTACCTCTCATAGGGAACCTTCACCATCTTTATGATGATTTAACCCATTCTGTTCTTCAACACCTTTCTAGTCAATATGGGCCAATCATGTTCATGCAATTAGGCTCGAGGCCTACTCTAGTAATCTCCTCAGCCGAAATGGCAAGAGAGGTTTTGAAAACTCATGACCTAGTTTTTCAGAGGGTCCCAATGTTTCAGTCTGTGAGGGATGAAGAGGTTGGAGTTATGATCAATTCAATAGCTCATGAGTGTAAGAGTATTGTCAATCTCACTGAAGTGACAGTTAGCTTGGCCAACAAGGTATTTTGTGTCGACGCTGTGCTTTTGGTAAGAAGTATGATGGGGAAGGTGAAACTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

429

Amino Acids

48.74

Weight (kDa)

9.16

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 294 1.3e-27 Cytochrome P450
p450 PF00067 304 - 376 1.8e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 546
AccI GTMKAC 3 cut(s) 292, 711, 1239
AccIII TCCGGA 1 cut(s) 787
AciI CCGC 1 cut(s) 544
AclWI GGATC 1 cut(s) 827
AcoI YGGCCR 1 cut(s) 1218
AcsI RAATTY 1 cut(s) 600
AcuI CTGAAG 2 cut(s) 222, 1221
AfiI CCNNNNNNNGG 4 cut(s) 119, 671, 871, 938
AjnI CCWGG 1 cut(s) 94
AleI CACNNNNGTG 1 cut(s) 1202
AluBI AGCT 4 cut(s) 327, 476, 1169, 1215
AluI AGCT 4 cut(s) 327, 476, 1169, 1215
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 3 cut(s) 238, 435, 497
AlwI GGATC 1 cut(s) 827
Ama87I CYCGRG 1 cut(s) 1033
Aor13HI TCCGGA 1 cut(s) 787
AoxI GGCC 4 cut(s) 779, 1007, 1037, 1218
ApoI RAATTY 1 cut(s) 600
AspS9I GGNCC 5 cut(s) 98, 188, 917, 1007, 1111
AsuHPI GGTGA 3 cut(s) 122, 941, 1288
AvaI CYCGRG 1 cut(s) 1033
AvaII GGWCC 4 cut(s) 98, 188, 917, 1111
BalI TGGCCA 1 cut(s) 1220
Bbv12I GWGCWC 1 cut(s) 9
BbvCI CCTCAGC 1 cut(s) 1057
BccI CCATC 5 cut(s) 141, 563, 664, 960, 1261
BciT130I CCWGG 1 cut(s) 96
BclI TGATCA 1 cut(s) 1155
BcoDI GTCTC 3 cut(s) 238, 435, 497
BfaI CTAG 7 cut(s) 102, 252, 894, 921, 996, 1046, 1097
BglI GCCNNNNNGGC 1 cut(s) 1067
BglII AGATCT 2 cut(s) 218, 799
BisI GCNGC 1 cut(s) 544
BlpI GCTNAGC 1 cut(s) 323
BlsI GCNGC 1 cut(s) 545
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 4 cut(s) 98, 188, 917, 1111
BmeT110I CYCGRG 1 cut(s) 1033
BmgT120I GGNCC 5 cut(s) 98, 188, 917, 1007, 1111
BmiI GGNNCC 4 cut(s) 125, 944, 1112, 1113
BmrFI CCNGG 1 cut(s) 96
BplI GAGNNNNNCTC 4 cut(s) 76, 108, 1027, 1059
BpmI CTGGAG 2 cut(s) 319, 597
Bpu10I CCTNAGC 1 cut(s) 1057
Bpu1102I GCTNAGC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 665
BsaWI WCCGGW 1 cut(s) 787
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 671, 871, 938
Bse1I ACTGG 3 cut(s) 177, 336, 1287
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 787
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 665
BseGI GGATG 2 cut(s) 675, 1141
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 4 cut(s) 119, 671, 871, 938
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 1 cut(s) 1071
BseNI ACTGG 3 cut(s) 177, 336, 1287
BseRI GAGGAG 1 cut(s) 1045
BshFI GGCC 4 cut(s) 781, 1009, 1039, 1220
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 1033
BsiSI CCGG 1 cut(s) 788
BslFI GGGAC 1 cut(s) 1097
BslI CCNNNNNNNGG 4 cut(s) 119, 671, 871, 938
BsmAI GTCTC 3 cut(s) 238, 435, 497
BsmBI CGTCTC 1 cut(s) 435
BsmFI GGGAC 1 cut(s) 1097
BsnI GGCC 4 cut(s) 781, 1009, 1039, 1220
BsoBI CYCGRG 1 cut(s) 1033
Bsp1286I GDGCHC 1 cut(s) 9
Bsp13I TCCGGA 1 cut(s) 787
Bsp143I GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
Bsp1720I GCTNAGC 1 cut(s) 323
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 1 cut(s) 544
BspANI GGCC 4 cut(s) 781, 1009, 1039, 1220
BspCNI CTCAG 1 cut(s) 1070
BspEI TCCGGA 1 cut(s) 787
BspHI TCATGA 3 cut(s) 271, 1090, 1171
BspLI GGNNCC 4 cut(s) 125, 944, 1112, 1113
BspPI GGATC 1 cut(s) 827
BsrBI CCGCTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 3 cut(s) 177, 336, 1287
BssECI CCNNGG 1 cut(s) 665
BssMI GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
BssT1I CCWWGG 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 3 cut(s) 533, 737, 1210
Bst6I CTCTTC 4 cut(s) 241, 441, 612, 1134
BstDEI CTNAG 3 cut(s) 323, 404, 1057
BstDSI CCRYGG 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 2 cut(s) 675, 1141
BstKTI GATC 6 cut(s) 195, 221, 393, 802, 835, 1158
BstMAI GTCTC 3 cut(s) 238, 435, 497
BstMBI GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
BstMWI GCNNNNNNNGC 2 cut(s) 64, 1067
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 94
BstX2I RGATCY 3 cut(s) 218, 799, 832
BstYI RGATCY 3 cut(s) 218, 799, 832
BsuRI GGCC 4 cut(s) 781, 1009, 1039, 1220
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 2 cut(s) 675, 1141
BtsIMutI CAGTG 4 cut(s) 343, 509, 538, 1197
CciI TCATGA 3 cut(s) 271, 1090, 1171
Cfr13I GGNCC 5 cut(s) 98, 188, 917, 1007, 1111
CpoI CGGWCCG 1 cut(s) 188
CseI GACGC 1 cut(s) 1250
CspI CGGWCCG 1 cut(s) 188
CviAII CATG 9 cut(s) 196, 272, 666, 794, 1015, 1021, 1091, 1172, 1287
DdeI CTNAG 3 cut(s) 323, 404, 1057
DpnI GATC 6 cut(s) 194, 220, 392, 801, 834, 1157
DpnII GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
DraI TTTAAA 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 1218
Eam1104I CTCTTC 4 cut(s) 241, 441, 612, 1134
EarI CTCTTC 4 cut(s) 241, 441, 612, 1134
Eco130I CCWWGG 1 cut(s) 665
Eco147I AGGCCT 2 cut(s) 781, 1039
Eco47I GGWCC 4 cut(s) 98, 188, 917, 1111
Eco57I CTGAAG 2 cut(s) 222, 1221
Eco88I CYCGRG 1 cut(s) 1033
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 1111
EcoRII CCWGG 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 665
ErhI CCWWGG 1 cut(s) 665
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 9 cut(s) 199, 275, 669, 797, 1018, 1024, 1094, 1175, 1290
FalI AAGNNNNNCTT 2 cut(s) 208, 240
FaqI GGGAC 1 cut(s) 1097
FatI CATG 9 cut(s) 195, 271, 665, 793, 1014, 1020, 1090, 1171, 1286
FauNDI CATATG 1 cut(s) 459
FbaI TGATCA 1 cut(s) 1155
FblI GTMKAC 3 cut(s) 292, 711, 1239
Fnu4HI GCNGC 1 cut(s) 544
FokI GGATG 2 cut(s) 682, 1148
Fsp4HI GCNGC 1 cut(s) 544
FspBI CTAG 7 cut(s) 102, 252, 894, 921, 996, 1046, 1097
GluI GCNGC 1 cut(s) 544
GsuI CTGGAG 2 cut(s) 319, 597
HaeIII GGCC 4 cut(s) 781, 1009, 1039, 1220
HapII CCGG 1 cut(s) 788
HgaI GACGC 1 cut(s) 1250
Hin1II CATG 9 cut(s) 199, 275, 669, 797, 1018, 1024, 1094, 1175, 1290
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HinfI GANTC 2 cut(s) 332, 418
HpaII CCGG 1 cut(s) 788
HphI GGTGA 3 cut(s) 122, 941, 1288
Hpy166II GTNNAC 3 cut(s) 293, 712, 1240
Hpy188I TCNGA 4 cut(s) 142, 655, 828, 1107
Hpy188III TCNNGA 7 cut(s) 49, 216, 272, 698, 788, 1091, 1172
Hpy8I GTNNAC 3 cut(s) 293, 712, 1240
Hpy99I CGWCG 1 cut(s) 1244
HpyAV CCTTC 5 cut(s) 99, 137, 918, 956, 1267
HpyCH4III ACNGT 3 cut(s) 533, 737, 1210
HpyCH4V TGCA 6 cut(s) 205, 314, 410, 509, 648, 1024
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 1067
HpyF3I CTNAG 3 cut(s) 323, 404, 1057
Hsp92II CATG 9 cut(s) 199, 275, 669, 797, 1018, 1024, 1094, 1175, 1290
KflI GGGWCCC 1 cut(s) 1111
Kpn2I TCCGGA 1 cut(s) 787
Ksp22I TGATCA 1 cut(s) 1155
Kzo9I GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
LmnI GCTCC 2 cut(s) 12, 790
MaeI CTAG 7 cut(s) 102, 252, 894, 921, 996, 1046, 1097
MaeIII GTNAC 1 cut(s) 1204
MalI GATC 6 cut(s) 194, 220, 392, 801, 834, 1157
MbiI CCGCTC 1 cut(s) 546
MboI GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
MboII GAAGA 7 cut(s) 72, 228, 458, 629, 809, 974, 1151
MflI RGATCY 3 cut(s) 218, 799, 832
MhlI GDGCHC 1 cut(s) 9
MlsI TGGCCA 1 cut(s) 1220
MluCI AATT 6 cut(s) 117, 206, 600, 608, 1025, 1159
MluNI TGGCCA 1 cut(s) 1220
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 4 cut(s) 433, 552, 711, 1126
Mox20I TGGCCA 1 cut(s) 1220
MroI TCCGGA 1 cut(s) 787
MscI TGGCCA 1 cut(s) 1220
MseI TTAA 2 cut(s) 723, 968
MslI CAYNNNNRTG 3 cut(s) 957, 1019, 1202
Msp20I TGGCCA 1 cut(s) 1220
MspI CCGG 1 cut(s) 788
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 2 cut(s) 64, 1067
NcoI CCATGG 1 cut(s) 665
NdeI CATATG 1 cut(s) 459
NdeII GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
NlaIII CATG 9 cut(s) 199, 275, 669, 797, 1018, 1024, 1094, 1175, 1290
NlaIV GGNNCC 4 cut(s) 125, 944, 1112, 1113
NmeAIII GCCGAG 1 cut(s) 527
NmuCI GTSAC 1 cut(s) 1204
OliI CACNNNNGTG 1 cut(s) 1202
PaeR7I CTCGAG 1 cut(s) 1033
PagI TCATGA 3 cut(s) 271, 1090, 1171
PceI AGGCCT 2 cut(s) 781, 1039
PfeI GAWTC 1 cut(s) 418
PkrI GCNGC 1 cut(s) 545
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PpuMI RGGWCCY 1 cut(s) 1111
Psp5II RGGWCCY 1 cut(s) 1111
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 4 cut(s) 125, 944, 1112, 1113
PspPI GGNCC 5 cut(s) 98, 188, 917, 1007, 1111
PspPPI RGGWCCY 1 cut(s) 1111
PspXI VCTCGAGB 1 cut(s) 1033
PsuI RGATCY 3 cut(s) 218, 799, 832
RseI CAYNNNNRTG 3 cut(s) 957, 1019, 1202
Rsr2I CGGWCCG 1 cut(s) 188
RsrII CGGWCCG 1 cut(s) 188
SalI GTCGAC 1 cut(s) 1238
SaqAI TTAA 2 cut(s) 723, 968
SatI GCNGC 1 cut(s) 544
Sau3AI GATC 6 cut(s) 192, 218, 390, 799, 832, 1155
Sau96I GGNCC 5 cut(s) 98, 188, 917, 1007, 1111
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 1033
SinI GGWCC 4 cut(s) 98, 188, 917, 1111
SlaI CTCGAG 1 cut(s) 1033
SmiMI CAYNNNNRTG 3 cut(s) 957, 1019, 1202
SmlI CTYRAG 2 cut(s) 214, 1033
SmoI CTYRAG 2 cut(s) 214, 1033
Sse9I AATT 6 cut(s) 117, 206, 600, 608, 1025, 1159
SseBI AGGCCT 2 cut(s) 781, 1039
SsiI CCGC 1 cut(s) 544
SspMI CTAG 7 cut(s) 102, 252, 894, 921, 996, 1046, 1097
StuI AGGCCT 2 cut(s) 781, 1039
StyD4I CCNGG 1 cut(s) 94
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 3 cut(s) 533, 737, 1210
TaqI TCGA 4 cut(s) 807, 820, 1034, 1239
TaqII GACCGA 1 cut(s) 205
TasI AATT 6 cut(s) 117, 206, 600, 608, 1025, 1159
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 723, 968
Tru9I TTAA 2 cut(s) 723, 968
TscAI CASTG 4 cut(s) 343, 516, 538, 1204
TseFI GTSAC 1 cut(s) 1204
Tsp45I GTSAC 1 cut(s) 1204
TspDTI ATGAA 5 cut(s) 68, 641, 810, 1009, 1152
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 4 cut(s) 343, 516, 538, 1204
VpaK11BI GGWCC 4 cut(s) 98, 188, 917, 1111
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 1 cut(s) 600
XcmI CCANNNNNNNNNTGG 1 cut(s) 672
XhoI CTCGAG 1 cut(s) 1033
XmiI GTMKAC 3 cut(s) 292, 711, 1239
XspI CTAG 7 cut(s) 102, 252, 894, 921, 996, 1046, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.