Rmu_ssc0000421.1_g000055

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000421.1
OG0001697
Physical Location & Seq
Reverse (-)
203049 .. 204682
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000421.1_g000055.1.cds

Sequence Viewer

Length: 1446 bp
atgaagcgaaggaagcaaaatgttcaagcaaagagattgcctcctggtcctagaaggctacctgtaattgggaaccttcaccatctttctgatgatttacctcattgtgcctttgaacacctttccagtcattacggaccgatcatgttcttgcaattaggctcaagatctactttagtagtctcttcggccgaaatgactagagaggttttcaaaactcatgacctcattttttcaggtagaccagatttcttgtatgttgcaaagaggcttagctatgactccagtgtatcattttctcccatcggtaagtattggagggaggttagaaagattgtgatcttggaactacttagcgcaaagcggattcaaatgtttcgttctgtgagagacgaagaggttggacatatgattgatgacatagctcatacttctaatggtctcatcaatctcaatgaactgacacatttcttatccaacagagtcttgtgccgctcggcttttggcaaaaagtatgatggtgctggaggtgttattagcaagaacagaatttttgaattgcttgaagaggcaatgaacttgttgggagggttctgtatatcggattttctgccatggatgggttggctaaacaagttcaatggtcttgaaaaaagggtagacaagtgttttaaagggttggacagtttctatgacaaagtgattgaggaacaccttgacccaaaaaggcctattccggagcatgaagatcttctcgatgtgctacttcgagttcagaaagatccaagtcaagcaatagcacttactaacgaccaaatcaagggtgtcatcactgacatatttattgcagggactgacacatcctcagccacagtggtttggacaatggctgagctgatcaggaatcccttaatgatgaggaaagcgcaagatgaggtgagaggagtggtcaaggcacaagggaaggtggaagaaagtgatctttctgaactcatgtacctaaagttagttatcaaagaaagttttagactacatccgccagtgccatcactagttccaagagtaactaccgagagttgcacaattgaagggtacgaaattcctgccaaaacaatggtgtttattcttgcaaaaatgattggaagggacccaaaatgttgggagaatccaaatgaattcttccctgaaagattcttggacagctcgattgactacaagggaaaccattttgagcttttgccatttggggcaggaaggaggggttgtcctggtatgaactttgctatgcagctagttgagcttacactagctaatctgctgtatcgttttgactgggaattgcctgatgggatgaaaagagaagacttgaacatggaagaagctgctgggattacagtgcagaacaaagttcctttatatctagcagctacaccagtacatctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

481

Amino Acids

54.76

Weight (kDa)

6.93

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 495
AccI GTMKAC 2 cut(s) 241, 660
AccIII TCCGGA 1 cut(s) 736
AciI CCGC 3 cut(s) 364, 493, 1037
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 3 cut(s) 549, 1098, 1175
AfaI GTAC 3 cut(s) 998, 1094, 1437
AfiI CCNNNNNNNGG 3 cut(s) 68, 620, 820
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 2 cut(s) 43, 1267
Alw26I GTCTC 3 cut(s) 187, 384, 446
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 1 cut(s) 854
Aor13HI TCCGGA 1 cut(s) 736
AoxI GGCC 2 cut(s) 189, 728
ApeKI GCWGC 3 cut(s) 1288, 1382, 1424
ApoI RAATTY 3 cut(s) 549, 1098, 1175
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 2 cut(s) 359, 928
AspS9I GGNCC 3 cut(s) 47, 137, 1147
AsuHPI GGTGA 2 cut(s) 71, 949
AvaII GGWCC 3 cut(s) 47, 137, 1147
BaeI ACNNNNGTAYC 2 cut(s) 980, 1013
BbsI GAAGAC 1 cut(s) 1368
BbvCI CCTCAGC 1 cut(s) 865
BbvI GCAGC 3 cut(s) 1300, 1369, 1436
BccI CCATC 6 cut(s) 90, 311, 512, 613, 1054, 1340
BcgI CGANNNNNNTGC 2 cut(s) 1085, 1119
BciT130I CCWGG 2 cut(s) 45, 1269
BclI TGATCA 1 cut(s) 897
BcoDI GTCTC 3 cut(s) 187, 384, 446
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 6 cut(s) 51, 201, 1052, 1292, 1307, 1421
BglII AGATCT 2 cut(s) 167, 748
BisI GCNGC 4 cut(s) 493, 1289, 1383, 1425
BlpI GCTNAGC 2 cut(s) 272, 891
BlsI GCNGC 4 cut(s) 494, 1290, 1384, 1426
Bme1390I CCNGG 2 cut(s) 45, 1269
Bme18I GGWCC 3 cut(s) 47, 137, 1147
BmgT120I GGNCC 3 cut(s) 47, 137, 1147
BmiI GGNNCC 3 cut(s) 74, 1148, 1149
BmrFI CCNGG 2 cut(s) 45, 1269
BmrI ACTGGG 1 cut(s) 1342
BmuI ACTGGG 1 cut(s) 1342
BpiI GAAGAC 1 cut(s) 1368
BplI GAGNNNNNCTC 2 cut(s) 25, 57
BpmI CTGGAG 2 cut(s) 268, 546
Bpu10I CCTNAGC 1 cut(s) 865
Bpu1102I GCTNAGC 2 cut(s) 272, 891
BpuEI CTTGAG 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 2 cut(s) 1249, 1279
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 620, 820
Bse1I ACTGG 5 cut(s) 126, 285, 1040, 1337, 1433
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 338
BseAI TCCGGA 1 cut(s) 736
BseBI CCWGG 2 cut(s) 45, 1269
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 4 cut(s) 624, 860, 1033, 1356
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 3 cut(s) 68, 620, 820
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 879, 882
BseNI ACTGG 5 cut(s) 126, 285, 1040, 1337, 1433
BseRI GAGGAG 1 cut(s) 957
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 3 cut(s) 1300, 1369, 1436
BseYI CCCAGC 1 cut(s) 1385
BsgI GTGCAG 1 cut(s) 1418
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 2 cut(s) 191, 730
BsiEI CGRYCG 1 cut(s) 192
BsiSI CCGG 1 cut(s) 737
BslFI GGGAC 2 cut(s) 865, 1160
BslI CCNNNNNNNGG 3 cut(s) 68, 620, 820
BsmAI GTCTC 3 cut(s) 187, 384, 446
BsmBI CGTCTC 1 cut(s) 384
BsmFI GGGAC 2 cut(s) 865, 1160
BsnI GGCC 2 cut(s) 191, 730
Bso31I GGTCTC 1 cut(s) 446
Bsp13I TCCGGA 1 cut(s) 736
Bsp143I GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
Bsp1720I GCTNAGC 2 cut(s) 272, 891
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 3 cut(s) 364, 493, 1037
BspANI GGCC 2 cut(s) 191, 730
BspCNI CTCAG 2 cut(s) 878, 883
BspEI TCCGGA 1 cut(s) 736
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 74, 1148, 1149
BspPI GGATC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 446
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 5 cut(s) 126, 285, 1040, 1337, 1433
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
BssT1I CCWWGG 1 cut(s) 614
Bst2UI CCWGG 2 cut(s) 45, 1269
Bst4CI ACNGT 3 cut(s) 686, 874, 1396
Bst6I CTCTTC 3 cut(s) 190, 390, 561
BstDEI CTNAG 4 cut(s) 272, 353, 865, 891
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 4 cut(s) 624, 860, 1033, 1356
BstHHI GCGC 2 cut(s) 359, 928
BstKTI GATC 7 cut(s) 144, 170, 342, 751, 784, 900, 982
BstMAI GTCTC 3 cut(s) 187, 384, 446
BstMBI GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 2 cut(s) 13, 1297
BstNI CCWGG 2 cut(s) 45, 1269
BstSCI CCNGG 2 cut(s) 43, 1267
BstV1I GCAGC 3 cut(s) 1300, 1369, 1436
BstV2I GAAGAC 1 cut(s) 1368
BstX2I RGATCY 3 cut(s) 167, 748, 781
BstXI CCANNNNNNTGG 2 cut(s) 1114, 1158
BstYI RGATCY 3 cut(s) 167, 748, 781
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 2 cut(s) 191, 730
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 4 cut(s) 624, 860, 1033, 1356
BtsIMutI CAGTG 5 cut(s) 292, 831, 879, 1047, 1401
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 1 cut(s) 220
CfoI GCGC 2 cut(s) 359, 928
Cfr13I GGNCC 3 cut(s) 47, 137, 1147
CpoI CGGWCCG 1 cut(s) 137
Csp6I GTAC 3 cut(s) 997, 1093, 1436
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 6 cut(s) 145, 221, 615, 743, 994, 1372
CviQI GTAC 3 cut(s) 997, 1093, 1436
DdeI CTNAG 4 cut(s) 272, 353, 865, 891
DpnI GATC 7 cut(s) 143, 169, 341, 750, 783, 899, 981
DpnII GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
DraI TTTAAA 1 cut(s) 673
EaeI YGGCCR 1 cut(s) 189
EagI CGGCCG 1 cut(s) 189
Eam1104I CTCTTC 3 cut(s) 190, 390, 561
EarI CTCTTC 3 cut(s) 190, 390, 561
EciI GGCGGA 1 cut(s) 1026
EclXI CGGCCG 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 614
Eco147I AGGCCT 1 cut(s) 730
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 3 cut(s) 47, 137, 1147
Eco52I CGGCCG 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 1147
EcoRI GAATTC 1 cut(s) 1175
EcoRII CCWGG 2 cut(s) 43, 1267
EcoT14I CCWWGG 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 614
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FaqI GGGAC 2 cut(s) 865, 1160
FatI CATG 6 cut(s) 144, 220, 614, 742, 993, 1371
FauNDI CATATG 1 cut(s) 408
FbaI TGATCA 1 cut(s) 897
FblI GTMKAC 2 cut(s) 241, 660
Fnu4HI GCNGC 4 cut(s) 493, 1289, 1383, 1425
FokI GGATG 4 cut(s) 631, 847, 1020, 1363
Fsp4HI GCNGC 4 cut(s) 493, 1289, 1383, 1425
FspBI CTAG 6 cut(s) 51, 201, 1052, 1292, 1307, 1421
GlaI GCGC 2 cut(s) 358, 927
GluI GCNGC 4 cut(s) 493, 1289, 1383, 1425
GsaI CCCAGC 1 cut(s) 1389
GsuI CTGGAG 2 cut(s) 268, 546
HaeIII GGCC 2 cut(s) 191, 730
HapII CCGG 1 cut(s) 737
HhaI GCGC 2 cut(s) 359, 928
Hin1II CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
Hin6I GCGC 2 cut(s) 357, 926
HinP1I GCGC 2 cut(s) 357, 926
HinfI GANTC 6 cut(s) 281, 367, 483, 904, 1165, 1191
HpaII CCGG 1 cut(s) 737
HphI GGTGA 2 cut(s) 71, 949
Hpy166II GTNNAC 2 cut(s) 242, 661
Hpy188I TCNGA 4 cut(s) 91, 604, 777, 988
Hpy188III TCNNGA 6 cut(s) 165, 221, 647, 737, 755, 901
Hpy8I GTNNAC 2 cut(s) 242, 661
HpyAV CCTTC 7 cut(s) 3, 48, 86, 958, 1082, 1137, 1248
HpyCH4III ACNGT 3 cut(s) 686, 874, 1396
HpyCH4V TGCA 7 cut(s) 154, 263, 848, 1080, 1130, 1288, 1399
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 1297
HpyF3I CTNAG 4 cut(s) 272, 353, 865, 891
Hsp92II CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
HspAI GCGC 2 cut(s) 357, 926
KflI GGGWCCC 1 cut(s) 1147
Kpn2I TCCGGA 1 cut(s) 736
Ksp22I TGATCA 1 cut(s) 897
Kzo9I GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
LmnI GCTCC 1 cut(s) 739
Lsp1109I GCAGC 3 cut(s) 1300, 1369, 1436
MaeI CTAG 6 cut(s) 51, 201, 1052, 1292, 1307, 1421
MaeIII GTNAC 1 cut(s) 1063
MalI GATC 7 cut(s) 143, 169, 341, 750, 783, 899, 981
MbiI CCGCTC 1 cut(s) 495
MboI GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
MboII GAAGA 9 cut(s) 177, 407, 578, 743, 758, 983, 1171, 1373, 1388
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 3 cut(s) 167, 748, 781
MluCI AATT 8 cut(s) 66, 155, 549, 557, 1083, 1098, 1175, 1337
MlyI GAGTC 2 cut(s) 275, 492
MmeI TCCRAC 3 cut(s) 382, 501, 660
MroI TCCGGA 1 cut(s) 736
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 2 cut(s) 672, 911
MspI CCGG 1 cut(s) 737
MspR9I CCNGG 2 cut(s) 45, 1269
MunI CAATTG 1 cut(s) 1083
MvaI CCWGG 2 cut(s) 45, 1269
MwoI GCNNNNNNNGC 2 cut(s) 13, 1297
NcoI CCATGG 1 cut(s) 614
NdeI CATATG 1 cut(s) 408
NdeII GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
NlaIII CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
NlaIV GGNNCC 3 cut(s) 74, 1148, 1149
NmeAIII GCCGAG 1 cut(s) 476
PagI TCATGA 1 cut(s) 220
PceI AGGCCT 1 cut(s) 730
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 4 cut(s) 367, 904, 1165, 1191
PkrI GCNGC 4 cut(s) 494, 1290, 1384, 1426
PleI GAGTC 2 cut(s) 275, 491
PpsI GAGTC 2 cut(s) 275, 491
PpuMI RGGWCCY 1 cut(s) 1147
Psp5II RGGWCCY 1 cut(s) 1147
Psp6I CCWGG 2 cut(s) 43, 1267
PspFI CCCAGC 1 cut(s) 1385
PspGI CCWGG 2 cut(s) 43, 1267
PspN4I GGNNCC 3 cut(s) 74, 1148, 1149
PspPI GGNCC 3 cut(s) 47, 137, 1147
PspPPI RGGWCCY 1 cut(s) 1147
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 3 cut(s) 167, 748, 781
RsaI GTAC 3 cut(s) 998, 1094, 1437
RsaNI GTAC 3 cut(s) 997, 1093, 1436
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SaqAI TTAA 2 cut(s) 672, 911
SatI GCNGC 4 cut(s) 493, 1289, 1383, 1425
Sau3AI GATC 7 cut(s) 141, 167, 339, 748, 781, 897, 979
Sau96I GGNCC 3 cut(s) 47, 137, 1147
SchI GAGTC 2 cut(s) 275, 492
ScrFI CCNGG 2 cut(s) 45, 1269
SinI GGWCC 3 cut(s) 47, 137, 1147
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
SpeI ACTAGT 1 cut(s) 1051
Sse9I AATT 8 cut(s) 66, 155, 549, 557, 1083, 1098, 1175, 1337
SseBI AGGCCT 1 cut(s) 730
SsiI CCGC 3 cut(s) 364, 493, 1037
SspMI CTAG 6 cut(s) 51, 201, 1052, 1292, 1307, 1421
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 2 cut(s) 43, 1267
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 3 cut(s) 686, 874, 1396
TaqI TCGA 3 cut(s) 756, 769, 1205
TaqII GACCGA 1 cut(s) 154
TasI AATT 8 cut(s) 66, 155, 549, 557, 1083, 1098, 1175, 1337
TatI WGTACW 1 cut(s) 1435
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 4 cut(s) 367, 904, 1165, 1191
Tru1I TTAA 2 cut(s) 672, 911
Tru9I TTAA 2 cut(s) 672, 911
TscAI CASTG 5 cut(s) 292, 838, 879, 1047, 1401
TseI GCWGC 3 cut(s) 1288, 1382, 1424
TspDTI ATGAA 7 cut(s) 17, 471, 590, 759, 1188, 1289, 1367
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 5 cut(s) 292, 838, 879, 1047, 1401
VpaK11BI GGWCC 3 cut(s) 47, 137, 1147
XapI RAATTY 3 cut(s) 549, 1098, 1175
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmiI GTMKAC 2 cut(s) 241, 660
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 6 cut(s) 51, 201, 1052, 1292, 1307, 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.