RchiOBHm_Chr7g0192131

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001697
Physical Location & Seq
Reverse (-)
10698030 .. 10700265
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17170

Sequence Viewer

Length: 1611 bp
ATGTTGTATTTCCCATCTGTGTTTCTATATGTTCAGAATCTCAAGTTTAAGTTTGTTATTACATCTAAGACAGGAACTCATCAATTAGCCATGAGTTCTTACTTATCCTTGTCCATGGTGCTCCTACTCTTTATTTTTCTTATACCCTTTACTTTCTTGCTCTTGATGAAGCGAAGGAAGCAAAATGTTCAAGCAAAGAGATTGCCTCCTGGTCCTAGAAGGCTACCTGTAATTGGGAACCTTCACCATCTTTCTGATGATTTACCTCATTGTGCCTTTGAACACCCTTCCAGTCATTACGGACCGATCATGTTCTTGCAATTAGGCTCAAGATCTACTTTAGTAGTCTCTTCGGCCGAAATGACTAGAGAGGTTTTCAAAACTCATGACCTCATTTTTTCAGGTAGACCAGAATTCTTGTATGTTGCAAAGAGGCTTAGCTATGACTCCAGTGTATCATTTTCTCCCATCGGTAAGTATTGGAGGGAGGTTAGAAAGATTGTGATCTTGGAACTACTTAGTGCAAAGCGGATTCAAATGTTTCGTTCTGTGAGAGACGAAGAGGTTGGACATATGATTGATGACATAGCTCATACTTCTAATGGTCTCATCAATCTCAATGAACTGACACATTTCTTATCCAACAGAGTCTTGTGCCGCTCGGCTTTTGGCAAAAAGTATGATGGTGCTGGAGGTGTTATTAGCAAGAACAGAATTTTTGAATTGCTTGAAGAGGCAATGAACTTGTTGGGAGGGTTCTGCATATCGGATTTTCTGCCATGGATGGGTTGGCTAAACAAGTTCAATGGTCTTGAAAAAAGGGTAGACAAGTGTTTTAAAGGGTTGGACAGTTTCTATGACAAAGTGATTGAGGAACACCTTGACCCAAAAAGGCCTACTCCGGAGCATGAAGATCTTGTCGATGTGCTACTTCGAGTTCAGAAAGATCCAAGTCAAGCAATAGCACTTACTAACGACCAAATCAAGGGTGTCATCACTGACATATTTATTGCAGGGACTGACACATCCTCAGCCACAGTGGTTTGGACAATGGCTGAGCTGATCAGGAATCCCTTAGTGATGAGGAACGCGCAAGATGAGGTGAGAGGAGTGGTCAAGGCACAAGGGAAGGTGGAAGAAAGTGATCTTTCTGAACTCATGTACCTAAAGTTAGTTATCAAAGAAAGTTTTAGACTACATCCGCCAGTGCCATCACTAGTTCCAAGAGTAACTACCGAGAGTTGCACAATTGAAGGGTACGAAATTCCTGCCAAAACAATGGTGTTTATTCTTGCAAAAATGATTGGAAGGGACCCAAAATGTTGGGAGAATCCAAATGAATTCTTCCCTGAAAGATTCTTGGACAGCTCGATTGACTACAAGGGAAACCATTTTGAGCTTTTGCCATTTGGGGCAGGAAGGAGGGGTTGTCCTGGTATGAACTTTGCTATGCAGCTAGTTGAGCTTACACTAGCTAATCTGCTGTATCGTTTTGACTGGGAATTGCCTGATGGGATGAAAAGAGAAGACTTGAACATGGAAGAAGCTGCTGGGATTACAGTGCAGAACAAAGTTCCTTTATATCTAGCAGCTACACCAGTACATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

536

Amino Acids

61.1

Weight (kDa)

7.66

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 69 - 521 2.2e-96 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 2 cut(s) 406, 825
AccII CGCG 1 cut(s) 1091
AccIII TCCGGA 1 cut(s) 901
AciI CCGC 3 cut(s) 529, 658, 1202
AclWI GGATC 1 cut(s) 941
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 4 cut(s) 413, 714, 1263, 1340
AfaI GTAC 3 cut(s) 1163, 1259, 1602
AfiI CCNNNNNNNGG 3 cut(s) 233, 785, 985
AhlI ACTAGT 1 cut(s) 1216
AjnI CCWGG 2 cut(s) 208, 1432
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 3 cut(s) 352, 549, 611
AlwI GGATC 1 cut(s) 941
AlwNI CAGNNNCTG 1 cut(s) 1019
Aor13HI TCCGGA 1 cut(s) 901
AoxI GGCC 2 cut(s) 354, 893
ApeKI GCWGC 3 cut(s) 1453, 1547, 1589
ApoI RAATTY 4 cut(s) 413, 714, 1263, 1340
AspLEI GCGC 1 cut(s) 1093
AspS9I GGNCC 3 cut(s) 212, 302, 1312
AsuHPI GGTGA 2 cut(s) 236, 1114
AvaII GGWCC 3 cut(s) 212, 302, 1312
BaeI ACNNNNGTAYC 2 cut(s) 1145, 1178
BbsI GAAGAC 1 cut(s) 1533
Bbv12I GWGCWC 1 cut(s) 123
BbvCI CCTCAGC 1 cut(s) 1030
BbvI GCAGC 3 cut(s) 1465, 1534, 1601
BccI CCATC 7 cut(s) 22, 255, 476, 677, 778, 1219, 1505
BcgI CGANNNNNNTGC 2 cut(s) 1250, 1284
BciT130I CCWGG 2 cut(s) 210, 1434
BclI TGATCA 1 cut(s) 1062
BcoDI GTCTC 3 cut(s) 352, 549, 611
BcuI ACTAGT 1 cut(s) 1216
BfaI CTAG 6 cut(s) 216, 366, 1217, 1457, 1472, 1586
BglII AGATCT 2 cut(s) 332, 913
BisI GCNGC 4 cut(s) 658, 1454, 1548, 1590
BlpI GCTNAGC 2 cut(s) 437, 1056
BlsI GCNGC 4 cut(s) 659, 1455, 1549, 1591
Bme1390I CCNGG 2 cut(s) 210, 1434
Bme18I GGWCC 3 cut(s) 212, 302, 1312
BmgT120I GGNCC 3 cut(s) 212, 302, 1312
BmiI GGNNCC 3 cut(s) 239, 1313, 1314
BmrFI CCNGG 2 cut(s) 210, 1434
BmrI ACTGGG 1 cut(s) 1507
BmuI ACTGGG 1 cut(s) 1507
BpiI GAAGAC 1 cut(s) 1533
BplI GAGNNNNNCTC 2 cut(s) 190, 222
BpmI CTGGAG 2 cut(s) 433, 711
Bpu10I CCTNAGC 1 cut(s) 1030
Bpu1102I GCTNAGC 2 cut(s) 437, 1056
BpuEI CTTGAG 2 cut(s) 26, 313
BsaBI GATNNNNATC 1 cut(s) 503
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 114, 779
BsaWI WCCGGW 1 cut(s) 901
BsaXI ACNNNNNCTCC 2 cut(s) 1414, 1444
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 785, 985
Bse1I ACTGG 5 cut(s) 291, 450, 1205, 1502, 1598
Bse3DI GCAATG 1 cut(s) 744
Bse8I GATNNNNATC 1 cut(s) 503
BseAI TCCGGA 1 cut(s) 901
BseBI CCWGG 2 cut(s) 210, 1434
BseDI CCNNGG 2 cut(s) 114, 779
BseGI GGATG 4 cut(s) 789, 1025, 1198, 1521
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 3 cut(s) 233, 785, 985
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 1044, 1047
BseNI ACTGG 5 cut(s) 291, 450, 1205, 1502, 1598
BseRI GAGGAG 1 cut(s) 1122
BseX3I CGGCCG 1 cut(s) 354
BseXI GCAGC 3 cut(s) 1465, 1534, 1601
BseYI CCCAGC 1 cut(s) 1550
BsgI GTGCAG 1 cut(s) 1583
Bsh1236I CGCG 1 cut(s) 1091
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 2 cut(s) 356, 895
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 902
BslFI GGGAC 2 cut(s) 1030, 1325
BslI CCNNNNNNNGG 3 cut(s) 233, 785, 985
BsmAI GTCTC 3 cut(s) 352, 549, 611
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 2 cut(s) 1030, 1325
BsnI GGCC 2 cut(s) 356, 895
Bso31I GGTCTC 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 123
Bsp13I TCCGGA 1 cut(s) 901
Bsp143I GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
Bsp1720I GCTNAGC 2 cut(s) 437, 1056
Bsp19I CCATGG 2 cut(s) 114, 779
BspACI CCGC 3 cut(s) 529, 658, 1202
BspANI GGCC 2 cut(s) 356, 895
BspCNI CTCAG 2 cut(s) 1043, 1048
BspEI TCCGGA 1 cut(s) 901
BspFNI CGCG 1 cut(s) 1091
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 3 cut(s) 239, 1313, 1314
BspPI GGATC 1 cut(s) 941
BspTNI GGTCTC 1 cut(s) 611
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 744
BsrI ACTGG 5 cut(s) 291, 450, 1205, 1502, 1598
BssECI CCNNGG 2 cut(s) 114, 779
BssMI GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
BssT1I CCWWGG 2 cut(s) 114, 779
Bst2UI CCWGG 2 cut(s) 210, 1434
Bst4CI ACNGT 3 cut(s) 851, 1039, 1561
Bst6I CTCTTC 3 cut(s) 355, 555, 726
BstDEI CTNAG 6 cut(s) 66, 437, 518, 1030, 1056, 1075
BstDSI CCRYGG 2 cut(s) 114, 779
BstF5I GGATG 4 cut(s) 789, 1025, 1198, 1521
BstFNI CGCG 1 cut(s) 1091
BstHHI GCGC 1 cut(s) 1093
BstKTI GATC 7 cut(s) 309, 335, 507, 916, 949, 1065, 1147
BstMAI GTCTC 3 cut(s) 352, 549, 611
BstMBI GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 2 cut(s) 178, 1462
BstNI CCWGG 2 cut(s) 210, 1434
BstSCI CCNGG 2 cut(s) 208, 1432
BstUI CGCG 1 cut(s) 1091
BstV1I GCAGC 3 cut(s) 1465, 1534, 1601
BstV2I GAAGAC 1 cut(s) 1533
BstX2I RGATCY 3 cut(s) 332, 913, 946
BstXI CCANNNNNNTGG 2 cut(s) 1279, 1323
BstYI RGATCY 3 cut(s) 332, 913, 946
BstZI CGGCCG 1 cut(s) 354
BsuRI GGCC 2 cut(s) 356, 895
BtgI CCRYGG 2 cut(s) 114, 779
BtsCI GGATG 4 cut(s) 789, 1025, 1198, 1521
BtsIMutI CAGTG 5 cut(s) 457, 996, 1044, 1212, 1566
CaiI CAGNNNCTG 1 cut(s) 1019
CciI TCATGA 1 cut(s) 385
CfoI GCGC 1 cut(s) 1093
Cfr13I GGNCC 3 cut(s) 212, 302, 1312
CpoI CGGWCCG 1 cut(s) 302
Csp6I GTAC 3 cut(s) 1162, 1258, 1601
CspI CGGWCCG 1 cut(s) 302
CviAII CATG 8 cut(s) 91, 115, 310, 386, 780, 908, 1159, 1537
CviQI GTAC 3 cut(s) 1162, 1258, 1601
DdeI CTNAG 6 cut(s) 66, 437, 518, 1030, 1056, 1075
DpnI GATC 7 cut(s) 308, 334, 506, 915, 948, 1064, 1146
DpnII GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
DraI TTTAAA 1 cut(s) 838
EaeI YGGCCR 1 cut(s) 354
EagI CGGCCG 1 cut(s) 354
Eam1104I CTCTTC 3 cut(s) 355, 555, 726
EarI CTCTTC 3 cut(s) 355, 555, 726
EciI GGCGGA 1 cut(s) 1191
EclXI CGGCCG 1 cut(s) 354
Eco130I CCWWGG 2 cut(s) 114, 779
Eco147I AGGCCT 1 cut(s) 895
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 3 cut(s) 212, 302, 1312
Eco52I CGGCCG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 1312
EcoRI GAATTC 2 cut(s) 413, 1340
EcoRII CCWGG 2 cut(s) 208, 1432
EcoT14I CCWWGG 2 cut(s) 114, 779
ErhI CCWWGG 2 cut(s) 114, 779
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 8 cut(s) 94, 118, 313, 389, 783, 911, 1162, 1540
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FaqI GGGAC 2 cut(s) 1030, 1325
FatI CATG 8 cut(s) 90, 114, 309, 385, 779, 907, 1158, 1536
FauNDI CATATG 1 cut(s) 573
FbaI TGATCA 1 cut(s) 1062
FblI GTMKAC 2 cut(s) 406, 825
Fnu4HI GCNGC 4 cut(s) 658, 1454, 1548, 1590
FokI GGATG 4 cut(s) 796, 1012, 1185, 1528
Fsp4HI GCNGC 4 cut(s) 658, 1454, 1548, 1590
FspBI CTAG 6 cut(s) 216, 366, 1217, 1457, 1472, 1586
GlaI GCGC 1 cut(s) 1092
GluI GCNGC 4 cut(s) 658, 1454, 1548, 1590
GsaI CCCAGC 1 cut(s) 1554
GsuI CTGGAG 2 cut(s) 433, 711
HaeIII GGCC 2 cut(s) 356, 895
HapII CCGG 1 cut(s) 902
HhaI GCGC 1 cut(s) 1093
Hin1II CATG 8 cut(s) 94, 118, 313, 389, 783, 911, 1162, 1540
Hin6I GCGC 1 cut(s) 1091
HinP1I GCGC 1 cut(s) 1091
HinfI GANTC 7 cut(s) 37, 446, 532, 648, 1069, 1330, 1356
HpaII CCGG 1 cut(s) 902
HphI GGTGA 2 cut(s) 236, 1114
Hpy166II GTNNAC 2 cut(s) 407, 826
Hpy188I TCNGA 5 cut(s) 36, 256, 769, 942, 1153
Hpy188III TCNNGA 6 cut(s) 163, 330, 386, 812, 902, 1066
Hpy8I GTNNAC 2 cut(s) 407, 826
HpyAV CCTTC 8 cut(s) 168, 213, 251, 297, 1123, 1247, 1302, 1413
HpyCH4III ACNGT 3 cut(s) 851, 1039, 1561
HpyCH4V TGCA 9 cut(s) 319, 428, 524, 762, 1013, 1245, 1295, 1453, 1564
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 1462
HpyF3I CTNAG 6 cut(s) 66, 437, 518, 1030, 1056, 1075
Hsp92II CATG 8 cut(s) 94, 118, 313, 389, 783, 911, 1162, 1540
HspAI GCGC 1 cut(s) 1091
KflI GGGWCCC 1 cut(s) 1312
Kpn2I TCCGGA 1 cut(s) 901
Ksp22I TGATCA 1 cut(s) 1062
Kzo9I GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
LmnI GCTCC 2 cut(s) 126, 904
Lsp1109I GCAGC 3 cut(s) 1465, 1534, 1601
MaeI CTAG 6 cut(s) 216, 366, 1217, 1457, 1472, 1586
MaeIII GTNAC 1 cut(s) 1228
MalI GATC 7 cut(s) 308, 334, 506, 915, 948, 1064, 1146
MbiI CCGCTC 1 cut(s) 660
MboI GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
MboII GAAGA 8 cut(s) 342, 572, 743, 923, 1148, 1336, 1538, 1553
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 3 cut(s) 332, 913, 946
MhlI GDGCHC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 440, 657
MmeI TCCRAC 3 cut(s) 547, 666, 825
MroI TCCGGA 1 cut(s) 901
MseI TTAA 2 cut(s) 48, 837
MspI CCGG 1 cut(s) 902
MspR9I CCNGG 2 cut(s) 210, 1434
MunI CAATTG 1 cut(s) 1248
MvaI CCWGG 2 cut(s) 210, 1434
MvnI CGCG 1 cut(s) 1091
MwoI GCNNNNNNNGC 2 cut(s) 178, 1462
NcoI CCATGG 2 cut(s) 114, 779
NdeI CATATG 1 cut(s) 573
NdeII GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
NlaIII CATG 8 cut(s) 94, 118, 313, 389, 783, 911, 1162, 1540
NlaIV GGNNCC 3 cut(s) 239, 1313, 1314
NmeAIII GCCGAG 1 cut(s) 641
PagI TCATGA 1 cut(s) 385
PceI AGGCCT 1 cut(s) 895
PfeI GAWTC 5 cut(s) 37, 532, 1069, 1330, 1356
PkrI GCNGC 4 cut(s) 659, 1455, 1549, 1591
PleI GAGTC 2 cut(s) 440, 656
PpsI GAGTC 2 cut(s) 440, 656
PpuMI RGGWCCY 1 cut(s) 1312
Psp5II RGGWCCY 1 cut(s) 1312
Psp6I CCWGG 2 cut(s) 208, 1432
PspFI CCCAGC 1 cut(s) 1550
PspGI CCWGG 2 cut(s) 208, 1432
PspN4I GGNNCC 3 cut(s) 239, 1313, 1314
PspPI GGNCC 3 cut(s) 212, 302, 1312
PspPPI RGGWCCY 1 cut(s) 1312
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 3 cut(s) 332, 913, 946
RsaI GTAC 3 cut(s) 1163, 1259, 1602
RsaNI GTAC 3 cut(s) 1162, 1258, 1601
Rsr2I CGGWCCG 1 cut(s) 302
RsrII CGGWCCG 1 cut(s) 302
SaqAI TTAA 2 cut(s) 48, 837
SatI GCNGC 4 cut(s) 658, 1454, 1548, 1590
Sau3AI GATC 7 cut(s) 306, 332, 504, 913, 946, 1062, 1144
Sau96I GGNCC 3 cut(s) 212, 302, 1312
SchI GAGTC 2 cut(s) 440, 657
ScrFI CCNGG 2 cut(s) 210, 1434
SduI GDGCHC 1 cut(s) 123
SinI GGWCC 3 cut(s) 212, 302, 1312
SmlI CTYRAG 2 cut(s) 41, 328
SmoI CTYRAG 2 cut(s) 41, 328
SpeI ACTAGT 1 cut(s) 1216
SseBI AGGCCT 1 cut(s) 895
SsiI CCGC 3 cut(s) 529, 658, 1202
SspMI CTAG 6 cut(s) 216, 366, 1217, 1457, 1472, 1586
StuI AGGCCT 1 cut(s) 895
StyD4I CCNGG 2 cut(s) 208, 1432
StyI CCWWGG 2 cut(s) 114, 779
TaaI ACNGT 3 cut(s) 851, 1039, 1561
TaqI TCGA 3 cut(s) 921, 934, 1370
TaqII GACCGA 1 cut(s) 319
TatI WGTACW 1 cut(s) 1600
TauI GCSGC 1 cut(s) 660
TfiI GAWTC 5 cut(s) 37, 532, 1069, 1330, 1356
Tru1I TTAA 2 cut(s) 48, 837
Tru9I TTAA 2 cut(s) 48, 837
TscAI CASTG 5 cut(s) 457, 1003, 1044, 1212, 1566
TseI GCWGC 3 cut(s) 1453, 1547, 1589
TspDTI ATGAA 7 cut(s) 182, 636, 755, 924, 1353, 1454, 1532
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 5 cut(s) 457, 1003, 1044, 1212, 1566
VpaK11BI GGWCC 3 cut(s) 212, 302, 1312
XapI RAATTY 4 cut(s) 413, 714, 1263, 1340
XcmI CCANNNNNNNNNTGG 1 cut(s) 786
XmiI GTMKAC 2 cut(s) 406, 825
XspI CTAG 6 cut(s) 216, 366, 1217, 1457, 1472, 1586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.