RLG00000004397

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001697
Physical Location & Seq
Forward (+)
59003212 .. 59004840
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004397

Sequence Viewer

Length: 1446 bp
ATGAGGCAAAGGAAGCAAAATGTTCAAGCAAAGAGGTTGCCTCCTGGTCCTAGAAGGCTACCTGTAATTGGGAACCTTCACCATCTTTCTGATGATTTACCTCATTGTGCCCTTGAACACCTTTCCAGTCAGTACGGACCGATCATGTTCTTGCAATTAGGCTCAAGATCTACTTTAGTAGTCTCTTCATCGGAAATGGCAAGAGAGGTTTTCAAAACACATGACCTCATTTTTTCAGGTAGACCAGATTTCTTGTATGTTGCAAAGAGGCTCAGCTATGACTCCAGTGTATCATTTTCTCCCATTGGTAAGTATTGGAGGGAGGTTAGAAAGATTGTGATCTTGGAACTACTTAGTGCAAAGCGGATTCAAATGTCTCGTTCTGTGAGAGACGAAGAGGTTGGACATATGATTGATGACGTAGCTCATACTTCTAATGGTCTCATCAATCTCAATGAGCTGACACATTTCTTATCCAACAGTGTCTTGTGCCGCTCGGCTTTTGGCAAAAAGTATGATGGTGCTGGAGGTGTTATTAGCAAGAACAGAATTCTTGAATTGCTAGAAGAGGCAATGAACTTGTTGGGAGGGTTCTGCATATCGGATTTTCTGCCATGGATGGGTTGGCTAAACAAGTTCAATGGTCTTGAAAAAAGGGTAGACAAGTGTTTTAAAGGGTTGGACAGTTTCTATGACAAAGTGATTGAGGAACACCTTGACCCAAAAAGGCCTATTCCGGAGCATGAAGATCTTGTCGATGTGCTACTTCGAGTTCAGAAAGATCCAAGTCAAGCACTAGCACTTACTAACGAGCAAATCAAGGGTGTCATCACTGACATATTTATTGCAGGGACTGACACATCCTCAGCCACAGTGGTATGGACAATGGCTGAGCTGACCAGGAATCCCTTAGTGATGAGGAAAGCTCAAGATGAGGTGAGAGGAGTCGTCAAGGCAAAAGGGAAGGTGGAAGAAAGTGATCTTTCTGAACTCATGTACCTAAAGTTAGTTATCAAAGAAAGTTTTAGACTACATCCGCCAGCGCCATCACTAGTTCCAAGAGTAACTACCGAGAGTTGCACAATTGAAGGGTACGAAATTCCTGCCAAAACAATGGTGTTTATTCTTGCAAAAATGATTGGAAGGGACCCAAAATGTTGGGAGAATCCAAATGAATTCTTCCCTGAAAGATTCTTGGACAGCTCAATTGACTACAAGGGAAACCATTTTGAGCTTTTGCCATTCGGGGCAGGAAGAAGGGGTTGTCCTGGTATGAACTTTGCTATGCAGCTAGTTGAGCTTACACTAGCTAATTTGCTGTATCGTTTTGACTGGGAATTGCCTAATGGGATGAAAAGAGAAGACTTGAACATGGAAGAAGCTGCTGGGATTACAGTGCAGAAGAAAGTTCCTTTATGTCTAGCAGCTACAGCAGTACATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

482

Amino Acids

54.39

Weight (kDa)

7.21

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 14 - 467 2.9e-97 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 495
AccI GTMKAC 2 cut(s) 241, 660
AccIII TCCGGA 1 cut(s) 736
AciI CCGC 3 cut(s) 364, 493, 1037
AclWI GGATC 1 cut(s) 776
AcsI RAATTY 3 cut(s) 549, 1098, 1175
AfaI GTAC 4 cut(s) 134, 998, 1094, 1437
AfiI CCNNNNNNNGG 2 cut(s) 68, 620
AgsI TTSAA 9 cut(s) 26, 116, 214, 371, 557, 640, 650, 1088, 1369
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 3 cut(s) 43, 899, 1267
Alw26I GTCTC 4 cut(s) 187, 381, 384, 446
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 1 cut(s) 854
Aor13HI TCCGGA 1 cut(s) 736
AoxI GGCC 1 cut(s) 728
ApeKI GCWGC 3 cut(s) 1288, 1382, 1424
ApoI RAATTY 3 cut(s) 549, 1098, 1175
AspLEI GCGC 1 cut(s) 1045
AspS9I GGNCC 3 cut(s) 47, 137, 1147
AsuHPI GGTGA 2 cut(s) 71, 949
AvaII GGWCC 3 cut(s) 47, 137, 1147
BaeGI GKGCMC 1 cut(s) 112
BaeI ACNNNNGTAYC 2 cut(s) 980, 1013
BbsI GAAGAC 1 cut(s) 1368
BbvCI CCTCAGC 1 cut(s) 865
BbvI GCAGC 3 cut(s) 1300, 1369, 1436
BccI CCATC 4 cut(s) 90, 512, 613, 1054
BcgI CGANNNNNNTGC 2 cut(s) 1085, 1119
BciT130I CCWGG 3 cut(s) 45, 901, 1269
BcoDI GTCTC 4 cut(s) 187, 381, 384, 446
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 7 cut(s) 51, 563, 797, 1052, 1292, 1307, 1421
BfmI CTRYAG 1 cut(s) 1428
BfoI RGCGCY 1 cut(s) 1046
BglII AGATCT 2 cut(s) 167, 748
BisI GCNGC 4 cut(s) 493, 1289, 1383, 1425
BlpI GCTNAGC 2 cut(s) 272, 891
BlsI GCNGC 4 cut(s) 494, 1290, 1384, 1426
Bme1390I CCNGG 3 cut(s) 45, 901, 1269
Bme18I GGWCC 3 cut(s) 47, 137, 1147
BmgT120I GGNCC 3 cut(s) 47, 137, 1147
BmiI GGNNCC 3 cut(s) 74, 1148, 1149
BmrFI CCNGG 3 cut(s) 45, 901, 1269
BmrI ACTGGG 1 cut(s) 1342
BmuI ACTGGG 1 cut(s) 1342
BpiI GAAGAC 1 cut(s) 1368
BplI GAGNNNNNCTC 4 cut(s) 25, 57, 910, 942
BpmI CTGGAG 2 cut(s) 268, 546
Bpu10I CCTNAGC 1 cut(s) 865
Bpu1102I GCTNAGC 2 cut(s) 272, 891
BpuEI CTTGAG 2 cut(s) 148, 912
BsaBI GATNNNNATC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 1 cut(s) 736
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 620
Bse1I ACTGG 3 cut(s) 126, 285, 1337
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 338
BseAI TCCGGA 1 cut(s) 736
BseBI CCWGG 3 cut(s) 45, 901, 1269
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 4 cut(s) 624, 860, 1033, 1356
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 2 cut(s) 68, 620
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 3 cut(s) 286, 879, 882
BseNI ACTGG 3 cut(s) 126, 285, 1337
BseRI GAGGAG 1 cut(s) 957
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 3 cut(s) 1300, 1369, 1436
BseYI CCCAGC 1 cut(s) 1385
BsgI GTGCAG 1 cut(s) 1418
BshFI GGCC 1 cut(s) 730
BsiSI CCGG 1 cut(s) 737
BslFI GGGAC 2 cut(s) 865, 1160
BslI CCNNNNNNNGG 2 cut(s) 68, 620
BsmAI GTCTC 4 cut(s) 187, 381, 384, 446
BsmBI CGTCTC 1 cut(s) 384
BsmFI GGGAC 2 cut(s) 865, 1160
BsnI GGCC 1 cut(s) 730
Bso31I GGTCTC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 112
Bsp13I TCCGGA 1 cut(s) 736
Bsp143I GATC 6 cut(s) 141, 167, 339, 748, 781, 979
Bsp1720I GCTNAGC 2 cut(s) 272, 891
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 3 cut(s) 364, 493, 1037
BspANI GGCC 1 cut(s) 730
BspCNI CTCAG 3 cut(s) 285, 878, 883
BspEI TCCGGA 1 cut(s) 736
BspLI GGNNCC 3 cut(s) 74, 1148, 1149
BspPI GGATC 1 cut(s) 776
BspTNI GGTCTC 1 cut(s) 446
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 3 cut(s) 126, 285, 1337
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 6 cut(s) 141, 167, 339, 748, 781, 979
BssT1I CCWWGG 1 cut(s) 614
Bst2UI CCWGG 3 cut(s) 45, 901, 1269
Bst4CI ACNGT 4 cut(s) 482, 686, 874, 1396
Bst6I CTCTTC 3 cut(s) 190, 390, 561
BstC8I GCNNGC 1 cut(s) 1041
BstDEI CTNAG 5 cut(s) 272, 353, 865, 891, 910
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 4 cut(s) 624, 860, 1033, 1356
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 1 cut(s) 1045
BstKTI GATC 6 cut(s) 144, 170, 342, 751, 784, 982
BstMAI GTCTC 4 cut(s) 187, 381, 384, 446
BstMBI GATC 6 cut(s) 141, 167, 339, 748, 781, 979
BstMWI GCNNNNNNNGC 3 cut(s) 13, 1297, 1430
BstNI CCWGG 3 cut(s) 45, 901, 1269
BstSCI CCNGG 3 cut(s) 43, 899, 1267
BstSFI CTRYAG 1 cut(s) 1428
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 1300, 1369, 1436
BstV2I GAAGAC 1 cut(s) 1368
BstX2I RGATCY 3 cut(s) 167, 748, 781
BstXI CCANNNNNNTGG 2 cut(s) 1114, 1158
BstYI RGATCY 3 cut(s) 167, 748, 781
BsuRI GGCC 1 cut(s) 730
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 4 cut(s) 624, 860, 1033, 1356
BtsIMutI CAGTG 5 cut(s) 292, 487, 831, 879, 1401
Cac8I GCNNGC 1 cut(s) 1041
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 1 cut(s) 1045
Cfr13I GGNCC 3 cut(s) 47, 137, 1147
CpoI CGGWCCG 1 cut(s) 137
Csp6I GTAC 4 cut(s) 133, 997, 1093, 1436
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 6 cut(s) 145, 221, 615, 743, 994, 1372
CviQI GTAC 4 cut(s) 133, 997, 1093, 1436
DdeI CTNAG 5 cut(s) 272, 353, 865, 891, 910
DpnI GATC 6 cut(s) 143, 169, 341, 750, 783, 981
DpnII GATC 6 cut(s) 141, 167, 339, 748, 781, 979
DraI TTTAAA 1 cut(s) 673
Eam1104I CTCTTC 3 cut(s) 190, 390, 561
EarI CTCTTC 3 cut(s) 190, 390, 561
EciI GGCGGA 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 614
Eco147I AGGCCT 1 cut(s) 730
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 3 cut(s) 47, 137, 1147
EcoO109I RGGNCCY 1 cut(s) 1147
EcoRI GAATTC 2 cut(s) 549, 1175
EcoRII CCWGG 3 cut(s) 43, 899, 1267
EcoT14I CCWWGG 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 614
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FaqI GGGAC 2 cut(s) 865, 1160
FatI CATG 6 cut(s) 144, 220, 614, 742, 993, 1371
FauNDI CATATG 1 cut(s) 408
FblI GTMKAC 2 cut(s) 241, 660
Fnu4HI GCNGC 4 cut(s) 493, 1289, 1383, 1425
FokI GGATG 4 cut(s) 631, 847, 1020, 1363
Fsp4HI GCNGC 4 cut(s) 493, 1289, 1383, 1425
FspBI CTAG 7 cut(s) 51, 563, 797, 1052, 1292, 1307, 1421
GlaI GCGC 1 cut(s) 1044
GluI GCNGC 4 cut(s) 493, 1289, 1383, 1425
GsaI CCCAGC 1 cut(s) 1389
GsuI CTGGAG 2 cut(s) 268, 546
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 1 cut(s) 730
HapII CCGG 1 cut(s) 737
HhaI GCGC 1 cut(s) 1045
Hin1II CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
Hin6I GCGC 1 cut(s) 1043
HinP1I GCGC 1 cut(s) 1043
HinfI GANTC 6 cut(s) 281, 367, 904, 945, 1165, 1191
HpaII CCGG 1 cut(s) 737
HphI GGTGA 2 cut(s) 71, 949
Hpy166II GTNNAC 2 cut(s) 242, 661
Hpy188I TCNGA 5 cut(s) 91, 193, 604, 777, 988
Hpy188III TCNNGA 5 cut(s) 165, 554, 647, 737, 929
Hpy8I GTNNAC 2 cut(s) 242, 661
HpyAV CCTTC 6 cut(s) 48, 86, 958, 1082, 1137, 1251
HpyCH4III ACNGT 4 cut(s) 482, 686, 874, 1396
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 9 cut(s) 154, 263, 359, 597, 848, 1080, 1130, 1288, 1399
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 1297, 1430
HpyF3I CTNAG 5 cut(s) 272, 353, 865, 891, 910
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
HspAI GCGC 1 cut(s) 1043
KflI GGGWCCC 1 cut(s) 1147
Kpn2I TCCGGA 1 cut(s) 736
Kzo9I GATC 6 cut(s) 141, 167, 339, 748, 781, 979
LmnI GCTCC 1 cut(s) 739
Lsp1109I GCAGC 3 cut(s) 1300, 1369, 1436
MaeI CTAG 7 cut(s) 51, 563, 797, 1052, 1292, 1307, 1421
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 1063
MalI GATC 6 cut(s) 143, 169, 341, 750, 783, 981
MbiI CCGCTC 1 cut(s) 495
MboI GATC 6 cut(s) 141, 167, 339, 748, 781, 979
MfeI CAATTG 2 cut(s) 1083, 1206
MflI RGATCY 3 cut(s) 167, 748, 781
MhlI GDGCHC 1 cut(s) 112
MlyI GAGTC 2 cut(s) 275, 954
MmeI TCCRAC 3 cut(s) 382, 501, 660
MroI TCCGGA 1 cut(s) 736
MseI TTAA 1 cut(s) 672
MspI CCGG 1 cut(s) 737
MspR9I CCNGG 3 cut(s) 45, 901, 1269
MunI CAATTG 2 cut(s) 1083, 1206
MvaI CCWGG 3 cut(s) 45, 901, 1269
MwoI GCNNNNNNNGC 3 cut(s) 13, 1297, 1430
NcoI CCATGG 1 cut(s) 614
NdeI CATATG 1 cut(s) 408
NdeII GATC 6 cut(s) 141, 167, 339, 748, 781, 979
NlaIII CATG 6 cut(s) 148, 224, 618, 746, 997, 1375
NlaIV GGNNCC 3 cut(s) 74, 1148, 1149
NmeAIII GCCGAG 1 cut(s) 476
PceI AGGCCT 1 cut(s) 730
PfeI GAWTC 4 cut(s) 367, 904, 1165, 1191
PkrI GCNGC 4 cut(s) 494, 1290, 1384, 1426
PleI GAGTC 2 cut(s) 275, 953
PpsI GAGTC 2 cut(s) 275, 953
PpuMI RGGWCCY 1 cut(s) 1147
Psp5II RGGWCCY 1 cut(s) 1147
Psp6I CCWGG 3 cut(s) 43, 899, 1267
PspFI CCCAGC 1 cut(s) 1385
PspGI CCWGG 3 cut(s) 43, 899, 1267
PspN4I GGNNCC 3 cut(s) 74, 1148, 1149
PspPI GGNCC 3 cut(s) 47, 137, 1147
PspPPI RGGWCCY 1 cut(s) 1147
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 3 cut(s) 167, 748, 781
RsaI GTAC 4 cut(s) 134, 998, 1094, 1437
RsaNI GTAC 4 cut(s) 133, 997, 1093, 1436
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 4 cut(s) 493, 1289, 1383, 1425
Sau3AI GATC 6 cut(s) 141, 167, 339, 748, 781, 979
Sau96I GGNCC 3 cut(s) 47, 137, 1147
SchI GAGTC 2 cut(s) 275, 954
ScrFI CCNGG 3 cut(s) 45, 901, 1269
SduI GDGCHC 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 1428
SinI GGWCC 3 cut(s) 47, 137, 1147
SmlI CTYRAG 2 cut(s) 163, 927
SmoI CTYRAG 2 cut(s) 163, 927
SpeI ACTAGT 1 cut(s) 1051
SseBI AGGCCT 1 cut(s) 730
SsiI CCGC 3 cut(s) 364, 493, 1037
SspMI CTAG 7 cut(s) 51, 563, 797, 1052, 1292, 1307, 1421
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 3 cut(s) 43, 899, 1267
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 4 cut(s) 482, 686, 874, 1396
TaiI ACGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 756, 769
TaqII GACCGA 1 cut(s) 154
TatI WGTACW 1 cut(s) 1435
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 4 cut(s) 367, 904, 1165, 1191
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 5 cut(s) 292, 487, 838, 879, 1401
TseI GCWGC 3 cut(s) 1288, 1382, 1424
TspDTI ATGAA 6 cut(s) 177, 590, 759, 1188, 1289, 1367
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 5 cut(s) 292, 487, 838, 879, 1401
VpaK11BI GGWCC 3 cut(s) 47, 137, 1147
XapI RAATTY 3 cut(s) 549, 1098, 1175
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmiI GTMKAC 2 cut(s) 241, 660
XspI CTAG 7 cut(s) 51, 563, 797, 1052, 1292, 1307, 1421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.