Rroxscaffold_3G00263280

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001697
Physical Location & Seq
Forward (+)
57246742 .. 57249721
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263280.1

Sequence Viewer

Length: 1275 bp
ATGAATCTTCAGTTTATATGTGCAGAACTGAGAAGGTTGTCTTCTCAGCCAATCCAAGAACTATTCGCCTCTTCTGGACAGCTCCCACTTCGAATGGTGTACAGGTCTCAAAACGACTCCAATAGGGCTGAAATTTGGAGATTTCAGAATCTCAAGTTTAAGAAGCAAAATGTTCAAGCAAAGAGGTTGCCTCCTGGTCCTAGAAGGCTACCTGTAATTGGGAACCTTCACCATCTTTCTGATGATTTACCTCATTGTGCCCTTGAACACCTTTCCAGTCATATTGGAGGGGAGGTTAGAAAGATTGTGATCTTGGAACTACTTAGTGCAAAGCGGATTCAAATGTTTCGTTCTGTGAGAGATGAAGAGGTTGGACATATGATTGATGACGTAGCTCATACTTCTAATGGTCTCATCAATCTCAATGAACTGACACATTTTTTATCCAACAGTGTCTTGTGCCGCTCGGCTTTTGGCAAAAAGTATGATGATGCTGGAGGCGTTATTAGCAAGAACAGAATTCTTGAATTGCTAGAAGAGGCAATGAACTTGTTGGGAGGGTTCTGCATATCGGATTTTCTGCCATGGATGCGTTGGCTAAACAAGTTCAATGGTCTTGAAAAAAGGGTAGACAAGTGTTTTAAAGGGTTGGACAGTTTCTATGACAAAGTGATTGAGGAACACCTTGACCCAAAAAGGCCTATTCCGGAGCATGAAGATCTTGTCGATGTGCTACTTCGAGACATATTTATTGCAGGGACTGACACATCCTCAGCCACAGTGGTGTGGACAATGGCTGAGCTGATCAGGAATCCCTTAGTGATGAGGAAAGCACAAGATGAGGTGAGAGGAGTCGTCAAGGCAAAAGGGAATGTGGAAGAAAGTGATCTTTCTGAACTCATGTACCTAAAGGTAGTTATCAAAGAAAGTTTTAGACTACATCCGCCAGTGCCATCACTAGTTCCAAGAGTAACTACCGAGAGTTGCACAATTGAAGGGTACGAAATTCCTGCCAAAACAATGGTGTTTATTCTTGCAAAAATGATTGGAAGGGACCCAAAATGTTGGGAGAATCCAAATGAATTCTTCCCTGAAAGATTCTTGGACAGCTCGATTGACTACAAGGGAAACCATTTTGAGCTTTTGCCATTTGGGGCAGGAAGGAGGGGTTGTCCTGGTATGAACTTTGCTATGCAGCTAGTTGAGCTTACACTAGCTAATTTGCTGTATCGTTTTGACTGGGAATTGCCTGATGGGATGAAAAAGAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

424

Amino Acids

48.55

Weight (kDa)

7.19

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 99 - 422 2.4e-68 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 465
AccI GTMKAC 1 cut(s) 630
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 3 cut(s) 334, 463, 944
AcsI RAATTY 4 cut(s) 132, 519, 1005, 1082
AfaI GTAC 3 cut(s) 101, 905, 1001
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 7 cut(s) 176, 266, 341, 527, 610, 620, 995
AhlI ACTAGT 1 cut(s) 958
AjnI CCWGG 2 cut(s) 193, 1174
AleI CACNNNNGTG 1 cut(s) 782
AluBI AGCT 8 cut(s) 82, 395, 802, 1110, 1141, 1198, 1207, 1217
AluI AGCT 8 cut(s) 82, 395, 802, 1110, 1141, 1198, 1207, 1217
Alw26I GTCTC 3 cut(s) 111, 416, 735
AlwNI CAGNNNCTG 1 cut(s) 761
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 1 cut(s) 698
ApeKI GCWGC 1 cut(s) 1195
ApoI RAATTY 4 cut(s) 132, 519, 1005, 1082
AspS9I GGNCC 2 cut(s) 197, 1054
AsuHPI GGTGA 2 cut(s) 221, 856
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 2 cut(s) 197, 1054
BaeGI GKGCMC 1 cut(s) 262
BaeI ACNNNNGTAYC 2 cut(s) 887, 920
BbsI GAAGAC 1 cut(s) 33
BbvCI CCTCAGC 1 cut(s) 772
BbvI GCAGC 1 cut(s) 1207
BccI CCATC 3 cut(s) 240, 961, 1247
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 2 cut(s) 195, 1176
BclI TGATCA 1 cut(s) 804
BcoDI GTCTC 3 cut(s) 111, 416, 735
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 5 cut(s) 201, 533, 959, 1199, 1214
BglII AGATCT 1 cut(s) 718
BisI GCNGC 2 cut(s) 463, 1196
BlpI GCTNAGC 1 cut(s) 798
BlsI GCNGC 2 cut(s) 464, 1197
Bme1390I CCNGG 2 cut(s) 195, 1176
Bme18I GGWCC 2 cut(s) 197, 1054
BmgT120I GGNCC 2 cut(s) 197, 1054
BmiI GGNNCC 3 cut(s) 224, 1055, 1056
BmrFI CCNGG 2 cut(s) 195, 1176
BmrI ACTGGG 1 cut(s) 1249
BmsI GCATC 2 cut(s) 481, 579
BmuI ACTGGG 1 cut(s) 1249
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 772
Bpu1102I GCTNAGC 1 cut(s) 798
Bpu14I TTCGAA 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 308
BsaI GGTCTC 2 cut(s) 111, 416
BsaJI CCNNGG 1 cut(s) 584
BsaWI WCCGGW 1 cut(s) 706
BsaXI ACNNNNNCTCC 4 cut(s) 279, 309, 1156, 1186
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 276, 947, 1244
Bse3DI GCAATG 1 cut(s) 549
Bse8I GATNNNNATC 1 cut(s) 308
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 2 cut(s) 195, 1176
BseDI CCNNGG 1 cut(s) 584
BseGI GGATG 4 cut(s) 594, 767, 940, 1263
BseJI GATNNNNATC 1 cut(s) 308
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 4 cut(s) 20, 59, 786, 789
BseNI ACTGG 3 cut(s) 276, 947, 1244
BseRI GAGGAG 1 cut(s) 864
BseSI GKGCMC 1 cut(s) 262
BseXI GCAGC 1 cut(s) 1207
BsgI GTGCAG 1 cut(s) 42
BshFI GGCC 1 cut(s) 700
BsiSI CCGG 1 cut(s) 707
BslFI GGGAC 2 cut(s) 772, 1067
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 3 cut(s) 111, 416, 735
BsmFI GGGAC 2 cut(s) 772, 1067
BsnI GGCC 1 cut(s) 700
Bso31I GGTCTC 2 cut(s) 111, 416
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 262
Bsp13I TCCGGA 1 cut(s) 706
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 4 cut(s) 309, 718, 804, 886
Bsp1720I GCTNAGC 1 cut(s) 798
Bsp19I CCATGG 1 cut(s) 584
BspACI CCGC 3 cut(s) 334, 463, 944
BspANI GGCC 1 cut(s) 700
BspCNI CTCAG 4 cut(s) 21, 58, 785, 790
BspEI TCCGGA 1 cut(s) 706
BspLI GGNNCC 3 cut(s) 224, 1055, 1056
BspT104I TTCGAA 1 cut(s) 91
BspTNI GGTCTC 2 cut(s) 111, 416
BsrBI CCGCTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 549
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 3 cut(s) 276, 947, 1244
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 4 cut(s) 309, 718, 804, 886
BssT1I CCWWGG 1 cut(s) 584
Bst2UI CCWGG 2 cut(s) 195, 1176
Bst4CI ACNGT 3 cut(s) 452, 656, 781
Bst6I CTCTTC 3 cut(s) 76, 360, 531
BstAUI TGTACA 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 91
BstDEI CTNAG 6 cut(s) 29, 45, 323, 772, 798, 817
BstDSI CCRYGG 1 cut(s) 584
BstF5I GGATG 4 cut(s) 594, 767, 940, 1263
BstKTI GATC 4 cut(s) 312, 721, 807, 889
BstMAI GTCTC 3 cut(s) 111, 416, 735
BstMBI GATC 4 cut(s) 309, 718, 804, 886
BstMWI GCNNNNNNNGC 3 cut(s) 507, 589, 1204
BstNI CCWGG 2 cut(s) 195, 1176
BstSCI CCNGG 2 cut(s) 193, 1174
BstSLI GKGCMC 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 1207
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 2 cut(s) 1021, 1065
BstYI RGATCY 1 cut(s) 718
BsuRI GGCC 1 cut(s) 700
BtgI CCRYGG 1 cut(s) 584
BtsCI GGATG 4 cut(s) 594, 767, 940, 1263
BtsIMutI CAGTG 3 cut(s) 457, 786, 954
CaiI CAGNNNCTG 1 cut(s) 761
Cfr13I GGNCC 2 cut(s) 197, 1054
Csp6I GTAC 3 cut(s) 100, 904, 1000
CviAII CATG 3 cut(s) 585, 713, 901
CviQI GTAC 3 cut(s) 100, 904, 1000
DdeI CTNAG 6 cut(s) 29, 45, 323, 772, 798, 817
DpnI GATC 4 cut(s) 311, 720, 806, 888
DpnII GATC 4 cut(s) 309, 718, 804, 886
DraI TTTAAA 1 cut(s) 643
Eam1104I CTCTTC 3 cut(s) 76, 360, 531
EarI CTCTTC 3 cut(s) 76, 360, 531
EciI GGCGGA 1 cut(s) 933
Eco130I CCWWGG 1 cut(s) 584
Eco147I AGGCCT 1 cut(s) 700
Eco31I GGTCTC 2 cut(s) 111, 416
Eco47I GGWCC 2 cut(s) 197, 1054
EcoO109I RGGNCCY 1 cut(s) 1054
EcoRI GAATTC 2 cut(s) 519, 1082
EcoRII CCWGG 2 cut(s) 193, 1174
EcoT14I CCWWGG 1 cut(s) 584
ErhI CCWWGG 1 cut(s) 584
FaeI CATG 3 cut(s) 588, 716, 904
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 2 cut(s) 772, 1067
FatI CATG 3 cut(s) 584, 712, 900
FauNDI CATATG 1 cut(s) 378
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 2 cut(s) 463, 1196
FokI GGATG 4 cut(s) 601, 754, 927, 1270
Fsp4HI GCNGC 2 cut(s) 463, 1196
FspBI CTAG 5 cut(s) 201, 533, 959, 1199, 1214
GluI GCNGC 2 cut(s) 463, 1196
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 1 cut(s) 700
HapII CCGG 1 cut(s) 707
Hin1II CATG 3 cut(s) 588, 716, 904
HinfI GANTC 8 cut(s) 4, 116, 148, 337, 811, 852, 1072, 1098
HpaII CCGG 1 cut(s) 707
HphI GGTGA 2 cut(s) 221, 856
Hpy166II GTNNAC 3 cut(s) 100, 631, 789
Hpy188I TCNGA 4 cut(s) 147, 241, 574, 895
Hpy188III TCNNGA 6 cut(s) 75, 524, 617, 707, 740, 808
Hpy8I GTNNAC 3 cut(s) 100, 631, 789
HpyAV CCTTC 6 cut(s) 27, 198, 236, 989, 1044, 1155
HpyCH4III ACNGT 3 cut(s) 452, 656, 781
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 7 cut(s) 23, 329, 567, 755, 987, 1037, 1195
HpyF10VI GCNNNNNNNGC 3 cut(s) 507, 589, 1204
HpyF3I CTNAG 6 cut(s) 29, 45, 323, 772, 798, 817
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 3 cut(s) 588, 716, 904
KflI GGGWCCC 1 cut(s) 1054
Kpn2I TCCGGA 1 cut(s) 706
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 4 cut(s) 309, 718, 804, 886
LmnI GCTCC 2 cut(s) 87, 709
Lsp1109I GCAGC 1 cut(s) 1207
LweI GCATC 2 cut(s) 481, 579
MaeI CTAG 5 cut(s) 201, 533, 959, 1199, 1214
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 1 cut(s) 970
MalI GATC 4 cut(s) 311, 720, 806, 888
MbiI CCGCTC 1 cut(s) 465
MboI GATC 4 cut(s) 309, 718, 804, 886
MboII GAAGA 7 cut(s) 33, 63, 377, 548, 728, 890, 1078
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 9 cut(s) 132, 216, 519, 527, 990, 1005, 1082, 1219, 1244
MlyI GAGTC 2 cut(s) 110, 861
MmeI TCCRAC 3 cut(s) 352, 471, 630
MroI TCCGGA 1 cut(s) 706
MseI TTAA 2 cut(s) 159, 642
MslI CAYNNNNRTG 1 cut(s) 782
MspI CCGG 1 cut(s) 707
MspR9I CCNGG 2 cut(s) 195, 1176
MunI CAATTG 1 cut(s) 990
MvaI CCWGG 2 cut(s) 195, 1176
MwoI GCNNNNNNNGC 3 cut(s) 507, 589, 1204
NcoI CCATGG 1 cut(s) 584
NdeI CATATG 1 cut(s) 378
NdeII GATC 4 cut(s) 309, 718, 804, 886
NlaIII CATG 3 cut(s) 588, 716, 904
NlaIV GGNNCC 3 cut(s) 224, 1055, 1056
NmeAIII GCCGAG 1 cut(s) 446
NspV TTCGAA 1 cut(s) 91
OliI CACNNNNGTG 1 cut(s) 782
PceI AGGCCT 1 cut(s) 700
PfeI GAWTC 6 cut(s) 4, 148, 337, 811, 1072, 1098
PkrI GCNGC 2 cut(s) 464, 1197
PleI GAGTC 2 cut(s) 110, 860
PpsI GAGTC 2 cut(s) 110, 860
PpuMI RGGWCCY 1 cut(s) 1054
Psp5II RGGWCCY 1 cut(s) 1054
Psp6I CCWGG 2 cut(s) 193, 1174
PspGI CCWGG 2 cut(s) 193, 1174
PspN4I GGNNCC 3 cut(s) 224, 1055, 1056
PspPI GGNCC 2 cut(s) 197, 1054
PspPPI RGGWCCY 1 cut(s) 1054
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 3 cut(s) 101, 905, 1001
RsaNI GTAC 3 cut(s) 100, 904, 1000
RseI CAYNNNNRTG 1 cut(s) 782
SaqAI TTAA 2 cut(s) 159, 642
SatI GCNGC 2 cut(s) 463, 1196
Sau3AI GATC 4 cut(s) 309, 718, 804, 886
Sau96I GGNCC 2 cut(s) 197, 1054
SchI GAGTC 2 cut(s) 110, 861
ScrFI CCNGG 2 cut(s) 195, 1176
SduI GDGCHC 1 cut(s) 262
SfaNI GCATC 2 cut(s) 481, 579
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 2 cut(s) 197, 1054
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SpeI ACTAGT 1 cut(s) 958
Sse9I AATT 9 cut(s) 132, 216, 519, 527, 990, 1005, 1082, 1219, 1244
SseBI AGGCCT 1 cut(s) 700
SsiI CCGC 3 cut(s) 334, 463, 944
SspMI CTAG 5 cut(s) 201, 533, 959, 1199, 1214
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 2 cut(s) 193, 1174
StyI CCWWGG 1 cut(s) 584
TaaI ACNGT 3 cut(s) 452, 656, 781
TaiI ACGT 1 cut(s) 393
TaqI TCGA 4 cut(s) 91, 726, 739, 1112
TasI AATT 9 cut(s) 132, 216, 519, 527, 990, 1005, 1082, 1219, 1244
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 6 cut(s) 4, 148, 337, 811, 1072, 1098
Tru1I TTAA 2 cut(s) 159, 642
Tru9I TTAA 2 cut(s) 159, 642
TscAI CASTG 3 cut(s) 457, 786, 954
TseI GCWGC 1 cut(s) 1195
TspDTI ATGAA 8 cut(s) 17, 378, 441, 560, 729, 1095, 1196, 1274
TspRI CASTG 3 cut(s) 457, 786, 954
VpaK11BI GGWCC 2 cut(s) 197, 1054
XapI RAATTY 4 cut(s) 132, 519, 1005, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 591
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 5 cut(s) 201, 533, 959, 1199, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.