Prupe.5G163300_v2.0.a1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001697
Physical Location & Seq
Reverse (-)
14264089 .. 14266166
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G163300.1

Sequence Viewer

Length: 1536 bp
ATGGATTCTCAATCTTCCTTGGTCATCCTCTTTGTTTTCCTCCTACCCTTTATTTTATTGCTCTTGATCAAGCAGAGGAAGAAAAGCTCTGCTTCTCAAGAGAGGAGGTTGCCTCCTGGTCCTAGGAGGCTACCTCTCATTGGGAACCTTCACAAGCTTTCTAATGGCTTACCTCATCATGTCCTTGAACACCTTGCCCTTCAATATGGACCCCTCATGTTCTTGAAATTAGGCTCAATATCTACTTTGGTAGTCTCTTCAGCCCATATGGCAAGAGAGATCTTCAAAACTCATGACCTTGTTTTTTCCGGTAGGCCAGTCTTGTATGTTGCAAAGAAGCTTAGCTATGGCTGTGTCAACCTATCATTTGCTCCCTATGGTGAGTATTGGAGGGAGATGAGGAAGATTGTGATCTTGGAACTGCTTAGTGAAAAGAGGGTTCGAATGTTTCAGTCTGTGAGGGATGAAGAGGTCACGCTTATGCTTGACTCTATAGCTCATTCTAAGGGTCTCATCAATCTCACTCAACTGACACTTTTCTTAGCAAACAAAGTCTTGTGCCGTTCGGCTTTCGGTAAAAAGTATGATGATGGAGGAGGAATTGGCAAGAGCAGAATTCATGGATTGCTTGAAGAGACAGGGGCCCTGTTAGGAGGATTTTTCATGTCAGATTTTCTACCATGGTTGAGTTGGTTAAACAAGTTCAATGGTCTTGAGAAAAAGGTAGAGAAGTGTTTTAAAGGCTTGGACAATTTCTATGACAGAGTGATTGAGGAACACCTTGATCCTAGAAGGCCTGAACCAGAGCAAGAAGATCTTGTCGATGTTCTGCTTCGGGTTCAGAGGGATGCCAGTCAAGCAATACCCCTCAGTAATGACCAAATTAAGGGTGTTCTCACTGACATGTTTATTGCAGGCACTGATACTTCCTCAGCCACACTGGTGTGGACAATGGCTGAACTGATTAGGAACCCCCTAGTGTTGAGGAAAGCACAAGATGAGGTGAGAGGAGTTTTAAAGGGAAAAGCAAAAGTGGAAGAAAGTGATCTTTCTGAACTCATGTACCTAAAGCTGGTCTTGAAGGAAAGTTTTAGACTACATCCAGCAGTGCCCTTACTACTTCCAAGAGAAACCTTAAAGAGTTGCACTATTGAAGGGTACGAAATTCCCACCACAACAATGGTGTTTATCCATGCAAAAATGATAGGAAAAGACCCAGAATGTTGGGAGAATCCAAATGAGTTCTGGCCTGAAAGATTCTTGGACAGCTCAATTGACTACAAAGGAAACCATTTTGAACTTTTACCATTTGGGGCAGGAAGGAGGGGTTGTCCTGGTATTAGCTTTAGTGTGAAGCTAATTGAGCTTGCACTTGCTAATCTCCTCTATCGTTTCGACTGGGAACTCCCTCATGGGGTGAGAAGAGAAGACTTGGACATGGAAGATGCTGCTGGGCTTGCAGTTAGCAAGAAAGTTCCTTTATTTCTAGCTGCTAAACCTGTGTATCCTTCCTTGACAAGCTATGTTGCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

512

Amino Acids

57.88

Weight (kDa)

8.52

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 779
AcsI RAATTY 2 cut(s) 615, 1164
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 1064, 1160
AfiI CCNNNNNNNGG 4 cut(s) 140, 886, 1072, 1414
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 9 cut(s) 188, 203, 226, 286, 632, 706, 1081, 1154, 1298
AjnI CCWGG 2 cut(s) 115, 1333
AleI CACNNNNGTG 2 cut(s) 941, 943
Alw26I GTCTC 3 cut(s) 259, 515, 629
AlwI GGATC 1 cut(s) 779
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 4 cut(s) 314, 642, 794, 1247
ApaI GGGCCC 1 cut(s) 646
ApeKI GCWGC 2 cut(s) 1448, 1490
ApoI RAATTY 2 cut(s) 615, 1164
AspA2I CCTAGG 1 cut(s) 122
AspS9I GGNCC 4 cut(s) 119, 209, 642, 643
AsuHPI GGTGA 3 cut(s) 392, 1015, 1429
AsuII TTCGAA 1 cut(s) 442
AvaII GGWCC 2 cut(s) 119, 209
AvrII CCTAGG 1 cut(s) 122
BaeGI GKGCMC 2 cut(s) 646, 1113
BaeI ACNNNNGTAYC 4 cut(s) 1046, 1079, 1487, 1520
BanII GRGCYC 1 cut(s) 646
BbsI GAAGAC 1 cut(s) 1434
BbvCI CCTCAGC 1 cut(s) 931
BbvI GCAGC 2 cut(s) 1435, 1477
BccI CCATC 1 cut(s) 584
BceAI ACGGC 1 cut(s) 546
BciT130I CCWGG 2 cut(s) 117, 1335
BciVI GTATCC 1 cut(s) 1515
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 3 cut(s) 259, 515, 629
BfaI CTAG 4 cut(s) 123, 789, 977, 1487
BfmI CTRYAG 1 cut(s) 492
BfuI GTATCC 1 cut(s) 1515
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 2 cut(s) 279, 814
BisI GCNGC 2 cut(s) 1449, 1491
BlnI CCTAGG 1 cut(s) 122
BlpI GCTNAGC 1 cut(s) 341
BlsI GCNGC 2 cut(s) 1450, 1492
Bme1390I CCNGG 2 cut(s) 117, 1335
Bme18I GGWCC 2 cut(s) 119, 209
BmgT120I GGNCC 4 cut(s) 119, 209, 642, 643
BmiI GGNNCC 5 cut(s) 146, 211, 643, 644, 971
BmrFI CCNGG 2 cut(s) 117, 1335
BmrI ACTGGG 1 cut(s) 1408
BmsI GCATC 2 cut(s) 838, 1435
BmuI ACTGGG 1 cut(s) 1408
BpiI GAAGAC 1 cut(s) 1434
BplI GAGNNNNNCTC 4 cut(s) 97, 129, 118, 150
Bpu10I CCTNAGC 1 cut(s) 931
Bpu1102I GCTNAGC 1 cut(s) 341
Bpu14I TTCGAA 1 cut(s) 442
BpuEI CTTGAG 2 cut(s) 81, 734
BsaBI GATNNNNATC 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 3 cut(s) 18, 122, 680
BsaWI WCCGGW 1 cut(s) 308
BsaXI ACNNNNNCTCC 2 cut(s) 1315, 1345
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 886, 1072, 1414
Bse1I ACTGG 4 cut(s) 317, 852, 945, 1403
Bse8I GATNNNNATC 1 cut(s) 410
BseBI CCWGG 2 cut(s) 117, 1335
BseDI CCNNGG 3 cut(s) 18, 122, 680
BseGI GGATG 4 cut(s) 24, 469, 853, 1099
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 4 cut(s) 140, 886, 1072, 1414
BseMII CTCAG 2 cut(s) 883, 945
BseNI ACTGG 4 cut(s) 317, 852, 945, 1403
BseRI GAGGAG 4 cut(s) 118, 609, 1023, 1373
BseSI GKGCMC 2 cut(s) 646, 1113
BseXI GCAGC 2 cut(s) 1435, 1477
BseYI CCCAGC 1 cut(s) 1451
BshFI GGCC 4 cut(s) 316, 644, 796, 1249
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 4 cut(s) 140, 886, 1072, 1414
BsmAI GTCTC 3 cut(s) 259, 515, 629
BsnI GGCC 4 cut(s) 316, 644, 796, 1249
Bso31I GGTCTC 1 cut(s) 515
Bsp119I TTCGAA 1 cut(s) 442
Bsp120I GGGCCC 1 cut(s) 642
Bsp1286I GDGCHC 2 cut(s) 646, 1113
Bsp143I GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
Bsp1720I GCTNAGC 1 cut(s) 341
Bsp19I CCATGG 1 cut(s) 680
BspANI GGCC 4 cut(s) 316, 644, 796, 1249
BspCNI CTCAG 2 cut(s) 882, 944
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 5 cut(s) 146, 211, 643, 644, 971
BspPI GGATC 1 cut(s) 779
BspT104I TTCGAA 1 cut(s) 442
BspTNI GGTCTC 1 cut(s) 515
BsrI ACTGG 4 cut(s) 317, 852, 945, 1403
BssECI CCNNGG 3 cut(s) 18, 122, 680
BssMI GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
BssT1I CCWWGG 3 cut(s) 18, 122, 680
Bst2UI CCWGG 2 cut(s) 117, 1335
Bst6I CTCTTC 4 cut(s) 262, 462, 627, 1417
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 3 cut(s) 916, 1368, 1458
BstDEI CTNAG 6 cut(s) 341, 425, 504, 541, 869, 931
BstDSI CCRYGG 1 cut(s) 680
BstF5I GGATG 4 cut(s) 24, 469, 853, 1099
BstKTI GATC 6 cut(s) 69, 282, 414, 787, 817, 1048
BstMAI GTCTC 3 cut(s) 259, 515, 629
BstMBI GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 269, 857, 1363, 1457
BstNI CCWGG 2 cut(s) 117, 1335
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 2 cut(s) 115, 1333
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 2 cut(s) 646, 1113
BstV1I GCAGC 2 cut(s) 1435, 1477
BstV2I GAAGAC 1 cut(s) 1434
BstX2I RGATCY 2 cut(s) 279, 814
BstXI CCANNNNNNTGG 2 cut(s) 1180, 1224
BstYI RGATCY 2 cut(s) 279, 814
BsuI GTATCC 1 cut(s) 1515
BsuRI GGCC 4 cut(s) 316, 644, 796, 1249
BtgI CCRYGG 1 cut(s) 680
BtsCI GGATG 4 cut(s) 24, 469, 853, 1099
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 4 cut(s) 897, 918, 938, 1113
Cac8I GCNNGC 3 cut(s) 916, 1368, 1458
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 4 cut(s) 119, 209, 642, 643
Csp6I GTAC 2 cut(s) 1063, 1159
CviQI GTAC 2 cut(s) 1063, 1159
DdeI CTNAG 6 cut(s) 341, 425, 504, 541, 869, 931
DpnI GATC 6 cut(s) 68, 281, 413, 786, 816, 1047
DpnII GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
DraI TTTAAA 2 cut(s) 739, 1017
Eam1104I CTCTTC 4 cut(s) 262, 462, 627, 1417
EarI CTCTTC 4 cut(s) 262, 462, 627, 1417
Eco130I CCWWGG 3 cut(s) 18, 122, 680
Eco147I AGGCCT 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 646
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 2 cut(s) 119, 209
Eco57I CTGAAG 1 cut(s) 243
EcoO109I RGGNCCY 2 cut(s) 642, 643
EcoRI GAATTC 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 115, 1333
EcoT14I CCWWGG 3 cut(s) 18, 122, 680
EcoT38I GRGCYC 1 cut(s) 646
ErhI CCWWGG 3 cut(s) 18, 122, 680
FalI AAGNNNNNCTT 6 cut(s) 76, 108, 801, 833, 1061, 1093
FauNDI CATATG 1 cut(s) 267
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 1449, 1491
FokI GGATG 4 cut(s) 11, 476, 860, 1086
FriOI GRGCYC 1 cut(s) 646
Fsp4HI GCNGC 2 cut(s) 1449, 1491
FspBI CTAG 4 cut(s) 123, 789, 977, 1487
GluI GCNGC 2 cut(s) 1449, 1491
GsaI CCCAGC 1 cut(s) 1455
HaeIII GGCC 4 cut(s) 316, 644, 796, 1249
HapII CCGG 1 cut(s) 309
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 2 cut(s) 155, 338
HinfI GANTC 4 cut(s) 5, 488, 1231, 1257
HpaII CCGG 1 cut(s) 309
HphI GGTGA 3 cut(s) 392, 1015, 1429
Hpy166II GTNNAC 2 cut(s) 358, 948
Hpy188I TCNGA 3 cut(s) 670, 843, 1054
Hpy188III TCNNGA 6 cut(s) 64, 98, 223, 293, 713, 1078
Hpy8I GTNNAC 2 cut(s) 358, 948
HpyAV CCTTC 7 cut(s) 158, 209, 786, 1075, 1148, 1314, 1518
HpyCH4V TGCA 7 cut(s) 332, 914, 1146, 1196, 1370, 1460, 1529
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 857, 1363, 1457
HpyF3I CTNAG 6 cut(s) 341, 425, 504, 541, 869, 931
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 2 cut(s) 1435, 1477
LweI GCATC 2 cut(s) 838, 1435
MaeI CTAG 4 cut(s) 123, 789, 977, 1487
MaeIII GTNAC 1 cut(s) 472
MalI GATC 6 cut(s) 68, 281, 413, 786, 816, 1047
MboI GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
MfeI CAATTG 1 cut(s) 1272
MflI RGATCY 2 cut(s) 279, 814
MhlI GDGCHC 2 cut(s) 646, 1113
MluCI AATT 8 cut(s) 227, 600, 615, 751, 882, 1164, 1272, 1359
MlyI GAGTC 1 cut(s) 482
MseI TTAA 5 cut(s) 695, 738, 885, 1016, 1136
MslI CAYNNNNRTG 5 cut(s) 479, 902, 941, 943, 1178
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 117, 1335
MunI CAATTG 1 cut(s) 1272
MvaI CCWGG 2 cut(s) 117, 1335
MwoI GCNNNNNNNGC 4 cut(s) 269, 857, 1363, 1457
NcoI CCATGG 1 cut(s) 680
NdeI CATATG 1 cut(s) 267
NdeII GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
NlaIV GGNNCC 5 cut(s) 146, 211, 643, 644, 971
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 1 cut(s) 907
NspV TTCGAA 1 cut(s) 442
OliI CACNNNNGTG 2 cut(s) 941, 943
PagI TCATGA 1 cut(s) 292
PceI AGGCCT 1 cut(s) 796
PciI ACATGT 1 cut(s) 903
PfeI GAWTC 3 cut(s) 5, 1231, 1257
PkrI GCNGC 2 cut(s) 1450, 1492
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PscI ACATGT 1 cut(s) 903
Psp6I CCWGG 2 cut(s) 115, 1333
PspFI CCCAGC 1 cut(s) 1451
PspGI CCWGG 2 cut(s) 115, 1333
PspN4I GGNNCC 5 cut(s) 146, 211, 643, 644, 971
PspOMI GGGCCC 1 cut(s) 642
PspPI GGNCC 4 cut(s) 119, 209, 642, 643
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 279, 814
RsaI GTAC 2 cut(s) 1064, 1160
RsaNI GTAC 2 cut(s) 1063, 1159
RseI CAYNNNNRTG 5 cut(s) 479, 902, 941, 943, 1178
SaqAI TTAA 5 cut(s) 695, 738, 885, 1016, 1136
SatI GCNGC 2 cut(s) 1449, 1491
Sau3AI GATC 6 cut(s) 66, 279, 411, 784, 814, 1045
Sau96I GGNCC 4 cut(s) 119, 209, 642, 643
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 117, 1335
SduI GDGCHC 2 cut(s) 646, 1113
SfaNI GCATC 2 cut(s) 838, 1435
SfcI CTRYAG 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 2 cut(s) 119, 209
SmiMI CAYNNNNRTG 5 cut(s) 479, 902, 941, 943, 1178
SmlI CTYRAG 2 cut(s) 96, 713
SmoI CTYRAG 2 cut(s) 96, 713
Sse9I AATT 8 cut(s) 227, 600, 615, 751, 882, 1164, 1272, 1359
SseBI AGGCCT 1 cut(s) 796
SspMI CTAG 4 cut(s) 123, 789, 977, 1487
StuI AGGCCT 1 cut(s) 796
StyD4I CCNGG 2 cut(s) 115, 1333
StyI CCWWGG 3 cut(s) 18, 122, 680
TaqI TCGA 3 cut(s) 442, 822, 1395
TasI AATT 8 cut(s) 227, 600, 615, 751, 882, 1164, 1272, 1359
TfiI GAWTC 3 cut(s) 5, 1231, 1257
Tru1I TTAA 5 cut(s) 695, 738, 885, 1016, 1136
Tru9I TTAA 5 cut(s) 695, 738, 885, 1016, 1136
TscAI CASTG 4 cut(s) 904, 925, 945, 1113
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 2 cut(s) 1448, 1490
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 3 cut(s) 480, 608, 652
TspRI CASTG 4 cut(s) 904, 925, 945, 1113
VpaK11BI GGWCC 2 cut(s) 119, 209
XapI RAATTY 2 cut(s) 615, 1164
XceI RCATGY 1 cut(s) 907
XcmI CCANNNNNNNNNTGG 3 cut(s) 687, 1177, 1242
XmaJI CCTAGG 1 cut(s) 122
XspI CTAG 4 cut(s) 123, 789, 977, 1487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.