Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000870

Physical Location & Seq

Genomic Coordinates Forward (+)

7965992 .. 7968286

Gene Structure 2295 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1067300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1194 bp

ATGTCTCAGGGAAGCTCCCCCGTCACTCTGTTTTCTCCGTACAAGATGGGCAAGTTCAGTCTCTCTCACAGGGTGGTTCTGGCGCCGCTGACGAGGTGCCGGGCGTTGAATGGACTGCCGCAACCAGCGCTGGCTGAGTACTACGTTCAGAGGTCAACTGACGGCGGCCTTCTCATTAGCGAAGCCGCTATAGTCTCCGACACTGGCGCCGGGATGCCCCGTGTTCCTGGGATTTACAACGACGAACAGGTGGAGGCATGGAAGAAGGTGGTGGATGCAATTCATGCCAAAGGCGCATTCATTTTCTGTCAACTTTGGCACGTTGGTCGTGCATCTCATGAAGTTTATCAACCTGGTGGGGGATTACCGATATCTTCAACCAACAATCCCATTTCAAAGAGACGGAATGTTCTGTTGCCAGATGGCTCTCATGGCACTTATCCTAAGCCTCGAGCCCTCGAAACCCAGGAAATTCCCCAAGTGGTTGAGCATTTTCGCCAGGGTGCGTTGAATGCCATTCAAGCAGGTTTTGATGGAGTTGAGATTCATGGGGCACATGGCTATATCATTGACCAATTCCTGAAAGACGGGATCAATGATCGAACTGATGAGTACGGTGGATCGCTAGAAAATCGGTGCAAATTTCTTATTCAGATTGTTCAAGCTGTGGTTGCAGCAATAGGTGCGGATAGAGTTGGGGTCAGAATCTCGCCGGCTATTGATCACTTGGATGCCATTGACTCCAAACCACTTATCTTAGGCTTGGCAGTGATCGAGAGGCTCAACAGGCTTCAACAGAATTGGGGCTCTAAACTGGCTTATCTCCATGTGACACAGCCTCGCTATTCAGCTCATGCCCAAGCAGAAGCTAGTAAACTCAGCAATGACGAAGAGGAAGCTGTGTTTATGAGGACTTTAAGAAACTCTTATCACGGTACATTCATAGCTAGCGGAGGATTCACTAGAGAGCTTGGAATGCAAGCAATTGCTTCCGGCGACGCTGATTTGGTGTCATACGGACGCCTTTTTATCTCGAACCCTGACTTGGTTTTGAGATTTAAGCTTAATGCCCCCTTGACGAAGTATAACAGGAAGACTTTCTACACACAAGACCCTGTTGTTGGGTACACAGACTACCCTTTTCTGAACAAATCCTATTACATGGATGAGGAGGAACGGCTCTCCCGTCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010035 GO:0010193 GO:0010817 GO:0016053 GO:0016491 GO:0016627 GO:0016628 GO:0016629 GO:0019752 GO:0022414 GO:0031407 GO:0031408 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042579 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046677 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0090567 GO:0099402 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00592 ko01100 ko01110 map00592 map01100 map01110

Pfam Domains

Protein Families

Oxidored_FMN

Protein Analysis

398

Amino Acids

43.89

Weight (kDa)

7.21

Isoelectric Point (pI)

34.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Oxidored_FMN	PF00724	10 - 357	6e-84	NADH:flavin oxidoreductase / NADH oxidase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000870)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G06050 AT2G06050 AT2G06050
fragaria_vesca	FvH4_1g08451 FvH4_1g08452 FvH4_1g08453 FvH4_1g08454 FvH4_1g08510 FvH4_1g08510 FvH4_1g08510 FvH4_1g08510
malus_domestica	MD00G1142600.v1.1 MD02G1089100.v1.1 MD12G1067300.v1.1 MD15G1216200.v1.1
prunus_persica	Prupe.7G200800_v2.0.a1
pyrus_communis	pycom02g07130 pycom15g19180
rosa_chinensis	RchiOBHm_Chr2g0094561 RchiOBHm_Chr2g0094611 RchiOBHm_Chr2g0094631 RchiOBHm_Chr2g0094641 RchiOBHm_Chr2g0150041 RchiOBHm_Chr7g0211621 RchiOBHm_Chr7g0230281
rosa_laevigata	RLG00000002990 RLG00000016504 RLG00000016506 RLG00000016507 RLG00000020462
rosa_multiflora	Rmu_co8133296.1_g000001 Rmu_co8390375.1_g000001 Rmu_sc0003760.1_g000017 Rmu_sc0004232.1_g000012 Rmu_sc0004359.1_g000033 Rmu_sc0004359.1_g000036 Rmu_sc0006422.1_g000001 Rmu_sc0009445.1_g000018 Rmu_sc0017068.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097490 Rroxscaffold_2G00147030 Rroxscaffold_2G00147040 Rroxscaffold_2G00147050 Rroxscaffold_3G00247670
rosa_rugosa	Rorug02G0045400 Rorug02G0045400 Rorug02G0045600 Rorug02G0045700 Rorug02G0418000 Rorug07G0126300
rosa_samantha	Rh2AG093100 Rh2BG093400 Rh2BG093700 Rh2BG489500 Rh2CG095800 Rh2CG096000 Rh2DG092200 Rh2DG092400 Rh7AG259000 Rh7BG253300 Rh7CG276100 Rh7DG266600
rosa_wichuraiana	Rw0G003550 Rw0G003560 Rw2G038940 Rw7G022140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	515
AccB1I	GGYRCC	3 cut(s)	82, 96, 206
AciI	CCGC	6 cut(s)	86, 119, 165, 186, 686, 951
AclWI	GGATC	2 cut(s)	599, 628
AcsI	RAATTY	2 cut(s)	471, 641
AcyI	GRCGYC	3 cut(s)	83, 207, 1021
AdeI	CACNNNGTG	1 cut(s)	73
AfaI	GTAC	5 cut(s)	41, 140, 614, 937, 1127
AfeI	AGCGCT	1 cut(s)	129
AfiI	CCNNNNNNNGG	3 cut(s)	359, 1045, 1121
AgsI	TTSAA	7 cut(s)	109, 378, 396, 511, 521, 662, 794
AjnI	CCWGG	4 cut(s)	226, 352, 465, 498
AjuI	GAANNNNNNNTTGG	4 cut(s)	281, 313, 1028, 1060
AluBI	AGCT	8 cut(s)	15, 665, 851, 869, 899, 947, 970, 1063
AluI	AGCT	8 cut(s)	15, 665, 851, 869, 899, 947, 970, 1063
Alw26I	GTCTC	4 cut(s)	9, 65, 199, 394
AlwI	GGATC	2 cut(s)	599, 628
Ama87I	CYCGRG	1 cut(s)	450
Aor51HI	AGCGCT	1 cut(s)	129
AoxI	GGCC	1 cut(s)	166
ApeKI	GCWGC	1 cut(s)	674
ApoI	RAATTY	2 cut(s)	471, 641
Asp700I	GAANNNNTTC	1 cut(s)	1097
AspLEI	GCGC	4 cut(s)	85, 130, 209, 296
AsuC2I	CCSGG	2 cut(s)	101, 211
AsuNHI	GCTAGC	1 cut(s)	947
AvaI	CYCGRG	1 cut(s)	450
BaeGI	GKGCMC	1 cut(s)	556
BanI	GGYRCC	3 cut(s)	82, 96, 206
BanII	GRGCYC	2 cut(s)	457, 809
BarI	GAAGNNNNNNTAC	2 cut(s)	1085, 1117
BbsI	GAAGAC	1 cut(s)	1100
BbvI	GCAGC	1 cut(s)	686
BccI	CCATC	3 cut(s)	40, 416, 527
BceAI	ACGGC	1 cut(s)	178
BcgI	CGANNNNNNTGC	2 cut(s)	308, 342
BciT130I	CCWGG	4 cut(s)	228, 354, 467, 500
BclI	TGATCA	1 cut(s)	721
BcnI	CCSGG	2 cut(s)	101, 211
BcoDI	GTCTC	4 cut(s)	9, 65, 199, 394
BfaI	CTAG	4 cut(s)	626, 870, 948, 963
BfmI	CTRYAG	1 cut(s)	189
BfoI	RGCGCY	3 cut(s)	86, 131, 210
BfuAI	ACCTGC	1 cut(s)	515
BisI	GCNGC	5 cut(s)	86, 119, 166, 186, 675
BlsI	GCNGC	5 cut(s)	87, 120, 167, 187, 676
BmcAI	AGTACT	1 cut(s)	140
Bme1390I	CCNGG	6 cut(s)	101, 211, 228, 354, 467, 500
BmeT110I	CYCGRG	1 cut(s)	450
BmiI	GGNNCC	3 cut(s)	84, 98, 208
BmrFI	CCNGG	6 cut(s)	101, 211, 228, 354, 467, 500
BmsI	GCATC	4 cut(s)	204, 265, 341, 721
BmtI	GCTAGC	1 cut(s)	951
BpiI	GAAGAC	1 cut(s)	1100
Bpu10I	CCTNAGC	1 cut(s)	444
BpuMI	CCSGG	2 cut(s)	101, 211
BsaHI	GRCGYC	3 cut(s)	83, 207, 1021
BsaJI	CCNNGG	3 cut(s)	227, 465, 499
Bsc4I	CCNNNNNNNGG	3 cut(s)	359, 1045, 1121
Bse118I	RCCGGY	1 cut(s)	712
Bse1I	ACTGG	2 cut(s)	208, 819
Bse3DI	GCAATG	1 cut(s)	889
BseBI	CCWGG	4 cut(s)	228, 354, 467, 500
BseDI	CCNNGG	3 cut(s)	227, 465, 499
BseGI	GGATG	4 cut(s)	219, 280, 736, 1171
BseLI	CCNNNNNNNGG	3 cut(s)	359, 1045, 1121
BseMI	GCAATG	1 cut(s)	889
BseMII	CTCAG	3 cut(s)	20, 126, 892
BseNI	ACTGG	2 cut(s)	208, 819
BseRI	GAGGAG	1 cut(s)	1184
BseSI	GKGCMC	1 cut(s)	556
BseXI	GCAGC	1 cut(s)	686
BshFI	GGCC	1 cut(s)	168
BshNI	GGYRCC	3 cut(s)	82, 96, 206
BsiHKCI	CYCGRG	1 cut(s)	450
BsiSI	CCGG	4 cut(s)	100, 210, 713, 993
BslI	CCNNNNNNNGG	3 cut(s)	359, 1045, 1121
BsmAI	GTCTC	4 cut(s)	9, 65, 199, 394
BsmBI	CGTCTC	1 cut(s)	394
BsmI	GAATGC	3 cut(s)	296, 517, 981
BsnI	GGCC	1 cut(s)	168
BsoBI	CYCGRG	1 cut(s)	450
Bsp1286I	GDGCHC	3 cut(s)	457, 556, 809
Bsp143I	GATC	5 cut(s)	591, 598, 620, 721, 771
BspACI	CCGC	6 cut(s)	86, 119, 165, 186, 686, 951
BspANI	GGCC	1 cut(s)	168
BspCNI	CTCAG	3 cut(s)	19, 127, 891
BspHI	TCATGA	1 cut(s)	337
BspLI	GGNNCC	3 cut(s)	84, 98, 208
BspMI	ACCTGC	1 cut(s)	515
BspOI	GCTAGC	1 cut(s)	951
BspPI	GGATC	2 cut(s)	599, 628
BspT107I	GGYRCC	3 cut(s)	82, 96, 206
BsrDI	GCAATG	1 cut(s)	889
BsrFI	RCCGGY	1 cut(s)	712
BsrI	ACTGG	2 cut(s)	208, 819
BssAI	RCCGGY	1 cut(s)	712
BssECI	CCNNGG	3 cut(s)	227, 465, 499
BssMI	GATC	5 cut(s)	591, 598, 620, 721, 771
BssNI	GRCGYC	3 cut(s)	83, 207, 1021
Bst2UI	CCWGG	4 cut(s)	228, 354, 467, 500
Bst4CI	ACNGT	2 cut(s)	617, 935
Bst6I	CTCTTC	1 cut(s)	885
BstACI	GRCGYC	3 cut(s)	83, 207, 1021
BstAPI	GCANNNNNTGC	2 cut(s)	284, 683
BstC8I	GCNNGC	4 cut(s)	132, 714, 949, 981
BstDEI	CTNAG	5 cut(s)	6, 135, 444, 757, 878
BstF5I	GGATG	4 cut(s)	219, 280, 736, 1171
BstH2I	RGCGCY	3 cut(s)	86, 131, 210
BstHHI	GCGC	4 cut(s)	85, 130, 209, 296
BstKTI	GATC	5 cut(s)	594, 601, 623, 724, 774
BstMAI	GTCTC	4 cut(s)	9, 65, 199, 394
BstMBI	GATC	5 cut(s)	591, 598, 620, 721, 771
BstNI	CCWGG	4 cut(s)	228, 354, 467, 500
BstSCI	CCNGG	6 cut(s)	99, 209, 226, 352, 465, 498
BstSFI	CTRYAG	1 cut(s)	189
BstSLI	GKGCMC	1 cut(s)	556
BstV1I	GCAGC	1 cut(s)	686
BstV2I	GAAGAC	1 cut(s)	1100
BsuRI	GGCC	1 cut(s)	168
BtsCI	GGATG	4 cut(s)	219, 280, 736, 1171
BtsI	GCAGTG	1 cut(s)	774
BtsIMutI	CAGTG	2 cut(s)	201, 774
BveI	ACCTGC	1 cut(s)	515
Cac8I	GCNNGC	4 cut(s)	132, 714, 949, 981
CciI	TCATGA	1 cut(s)	337
CfoI	GCGC	4 cut(s)	85, 130, 209, 296
Cfr10I	RCCGGY	1 cut(s)	712
CseI	GACGC	2 cut(s)	1007, 1029
CsiI	ACCWGGT	1 cut(s)	352
Csp6I	GTAC	5 cut(s)	40, 139, 613, 936, 1126
CviAII	CATG	9 cut(s)	258, 284, 338, 431, 548, 557, 827, 854, 1162
CviQI	GTAC	5 cut(s)	40, 139, 613, 936, 1126
DdeI	CTNAG	5 cut(s)	6, 135, 444, 757, 878
DinI	GGCGCC	2 cut(s)	84, 208
DpnI	GATC	5 cut(s)	593, 600, 622, 723, 773
DpnII	GATC	5 cut(s)	591, 598, 620, 721, 771
DraIII	CACNNNGTG	1 cut(s)	73
Eam1104I	CTCTTC	1 cut(s)	885
EarI	CTCTTC	1 cut(s)	885
Eco24I	GRGCYC	2 cut(s)	457, 809
Eco32I	GATATC	1 cut(s)	372
Eco47III	AGCGCT	1 cut(s)	129
Eco88I	CYCGRG	1 cut(s)	450
EcoRII	CCWGG	4 cut(s)	226, 352, 465, 498
EcoRV	GATATC	1 cut(s)	372
EcoT38I	GRGCYC	2 cut(s)	457, 809
EgeI	GGCGCC	2 cut(s)	84, 208
EheI	GGCGCC	2 cut(s)	84, 208
Esp3I	CGTCTC	1 cut(s)	394
FaeI	CATG	9 cut(s)	261, 287, 341, 434, 551, 560, 830, 857, 1165
FalI	AAGNNNNNCTT	2 cut(s)	910, 942
FatI	CATG	9 cut(s)	257, 283, 337, 430, 547, 556, 826, 853, 1161
FbaI	TGATCA	1 cut(s)	721
Fnu4HI	GCNGC	5 cut(s)	86, 119, 166, 186, 675
FokI	GGATG	4 cut(s)	226, 287, 743, 1178
FriOI	GRGCYC	2 cut(s)	457, 809
Fsp4HI	GCNGC	5 cut(s)	86, 119, 166, 186, 675
FspBI	CTAG	4 cut(s)	626, 870, 948, 963
GlaI	GCGC	4 cut(s)	84, 129, 208, 295
GluI	GCNGC	5 cut(s)	86, 119, 166, 186, 675
HaeII	RGCGCY	3 cut(s)	86, 131, 210
HaeIII	GGCC	1 cut(s)	168
HapII	CCGG	4 cut(s)	100, 210, 713, 993
HgaI	GACGC	2 cut(s)	1007, 1029
HhaI	GCGC	4 cut(s)	85, 130, 209, 296
Hin1I	GRCGYC	3 cut(s)	83, 207, 1021
Hin1II	CATG	9 cut(s)	261, 287, 341, 434, 551, 560, 830, 857, 1165
Hin6I	GCGC	4 cut(s)	83, 128, 207, 294
HinP1I	GCGC	4 cut(s)	83, 128, 207, 294
HincII	GTYRAC	2 cut(s)	156, 311
HindII	GTYRAC	2 cut(s)	156, 311
HindIII	AAGCTT	1 cut(s)	1061
HinfI	GANTC	4 cut(s)	544, 705, 740, 957
HpaII	CCGG	4 cut(s)	100, 210, 713, 993
Hpy166II	GTNNAC	4 cut(s)	156, 311, 875, 1128
Hpy188I	TCNGA	5 cut(s)	150, 199, 654, 704, 1146
Hpy188III	TCNNGA	4 cut(s)	338, 580, 775, 1033
Hpy8I	GTNNAC	4 cut(s)	156, 311, 875, 1128
Hpy99I	CGWCG	2 cut(s)	245, 1001
HpyAV	CCTTC	2 cut(s)	179, 259
HpyCH4III	ACNGT	2 cut(s)	617, 935
HpyCH4IV	ACGT	2 cut(s)	144, 321
HpyCH4V	TGCA	5 cut(s)	278, 332, 639, 674, 979
HpyF3I	CTNAG	5 cut(s)	6, 135, 444, 757, 878
HpySE526I	ACGT	2 cut(s)	144, 321
Hsp92I	GRCGYC	3 cut(s)	83, 207, 1021
Hsp92II	CATG	9 cut(s)	261, 287, 341, 434, 551, 560, 830, 857, 1165
HspAI	GCGC	4 cut(s)	83, 128, 207, 294
KasI	GGCGCC	2 cut(s)	82, 206
KroI	GCCGGC	1 cut(s)	712
KroNI	GCCGGC	1 cut(s)	714
Ksp22I	TGATCA	1 cut(s)	721
Kzo9I	GATC	5 cut(s)	591, 598, 620, 721, 771
LmnI	GCTCC	1 cut(s)	20
Lsp1109I	GCAGC	1 cut(s)	686
LweI	GCATC	4 cut(s)	204, 265, 341, 721
MabI	ACCWGGT	1 cut(s)	352
MaeI	CTAG	4 cut(s)	626, 870, 948, 963
MaeII	ACGT	2 cut(s)	144, 321
MaeIII	GTNAC	2 cut(s)	22, 829
MalI	GATC	5 cut(s)	593, 600, 622, 723, 773
MboI	GATC	5 cut(s)	591, 598, 620, 721, 771
MboII	GAAGA	4 cut(s)	274, 366, 902, 1105
MfeI	CAATTG	1 cut(s)	984
MhlI	GDGCHC	3 cut(s)	457, 556, 809
MluCI	AATT	6 cut(s)	279, 471, 575, 641, 799, 984
Mly113I	GGCGCC	2 cut(s)	83, 207
MlyI	GAGTC	1 cut(s)	734
MmeI	TCCRAC	1 cut(s)	222
MroNI	GCCGGC	1 cut(s)	712
MroXI	GAANNNNTTC	1 cut(s)	1097
MseI	TTAA	3 cut(s)	917, 1059, 1065
MslI	CAYNNNNRTG	1 cut(s)	729
MspA1I	CMGCKG	1 cut(s)	88
MspI	CCGG	4 cut(s)	100, 210, 713, 993
MspR9I	CCNGG	6 cut(s)	101, 211, 228, 354, 467, 500
MunI	CAATTG	1 cut(s)	984
Mva1269I	GAATGC	3 cut(s)	296, 517, 981
MvaI	CCWGG	4 cut(s)	228, 354, 467, 500
NaeI	GCCGGC	1 cut(s)	714
NarI	GGCGCC	2 cut(s)	83, 207
NciI	CCSGG	2 cut(s)	101, 211
NdeII	GATC	5 cut(s)	591, 598, 620, 721, 771
NgoMIV	GCCGGC	1 cut(s)	712
NheI	GCTAGC	1 cut(s)	947
NlaIII	CATG	9 cut(s)	261, 287, 341, 434, 551, 560, 830, 857, 1165
NlaIV	GGNNCC	3 cut(s)	84, 98, 208
NmuCI	GTSAC	2 cut(s)	22, 829
PaeR7I	CTCGAG	1 cut(s)	450
PagI	TCATGA	1 cut(s)	337
PcsI	WCGNNNNNNNCGW	1 cut(s)	1183
PctI	GAATGC	3 cut(s)	296, 517, 981
PdiI	GCCGGC	1 cut(s)	714
PdmI	GAANNNNTTC	1 cut(s)	1097
PfeI	GAWTC	3 cut(s)	544, 705, 957
PkrI	GCNGC	5 cut(s)	87, 120, 167, 187, 676
PleI	GAGTC	1 cut(s)	734
PluTI	GGCGCC	2 cut(s)	86, 210
PpsI	GAGTC	1 cut(s)	734
Psp6I	CCWGG	4 cut(s)	226, 352, 465, 498
PspGI	CCWGG	4 cut(s)	226, 352, 465, 498
PspN4I	GGNNCC	3 cut(s)	84, 98, 208
PspXI	VCTCGAGB	1 cut(s)	450
RsaI	GTAC	5 cut(s)	41, 140, 614, 937, 1127
RsaNI	GTAC	5 cut(s)	40, 139, 613, 936, 1126
RseI	CAYNNNNRTG	1 cut(s)	729
SaqAI	TTAA	3 cut(s)	917, 1059, 1065
SatI	GCNGC	5 cut(s)	86, 119, 166, 186, 675
Sau3AI	GATC	5 cut(s)	591, 598, 620, 721, 771
ScaI	AGTACT	1 cut(s)	140
SchI	GAGTC	1 cut(s)	734
ScrFI	CCNGG	6 cut(s)	101, 211, 228, 354, 467, 500
SduI	GDGCHC	3 cut(s)	457, 556, 809
SexAI	ACCWGGT	1 cut(s)	352
SfaNI	GCATC	4 cut(s)	204, 265, 341, 721
SfcI	CTRYAG	1 cut(s)	189
SfoI	GGCGCC	2 cut(s)	84, 208
Sfr274I	CTCGAG	1 cut(s)	450
SlaI	CTCGAG	1 cut(s)	450
SmiMI	CAYNNNNRTG	1 cut(s)	729
SmlI	CTYRAG	1 cut(s)	450
SmoI	CTYRAG	1 cut(s)	450
Sse9I	AATT	6 cut(s)	279, 471, 575, 641, 799, 984
SsiI	CCGC	6 cut(s)	86, 119, 165, 186, 686, 951
SspDI	GGCGCC	2 cut(s)	82, 206
SspMI	CTAG	4 cut(s)	626, 870, 948, 963
StyD4I	CCNGG	6 cut(s)	99, 209, 226, 352, 465, 498
TaaI	ACNGT	2 cut(s)	617, 935
TaiI	ACGT	2 cut(s)	147, 324
TaqI	TCGA	5 cut(s)	451, 459, 601, 774, 1034
TasI	AATT	6 cut(s)	279, 471, 575, 641, 799, 984
TatI	WGTACW	1 cut(s)	138
TauI	GCSGC	4 cut(s)	88, 121, 168, 188
TfiI	GAWTC	3 cut(s)	544, 705, 957
Tru1I	TTAA	3 cut(s)	917, 1059, 1065
Tru9I	TTAA	3 cut(s)	917, 1059, 1065
TscAI	CASTG	2 cut(s)	208, 774
TseFI	GTSAC	2 cut(s)	22, 829
TseI	GCWGC	1 cut(s)	674
Tsp45I	GTSAC	2 cut(s)	22, 829
TspDTI	ATGAA	5 cut(s)	272, 289, 354, 536, 931
TspGWI	ACGGA	3 cut(s)	27, 418, 1032
TspRI	CASTG	2 cut(s)	208, 774
XapI	RAATTY	2 cut(s)	471, 641
XhoI	CTCGAG	1 cut(s)	450
XmnI	GAANNNNTTC	1 cut(s)	1097
XspI	CTAG	4 cut(s)	626, 870, 948, 963
ZrmI	AGTACT	1 cut(s)	140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.