Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0001522

Physical Location & Seq

Genomic Coordinates Forward (+)

300985 .. 303258

Gene Structure 2274 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g00240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGTTACGCCCATGTGCTCTCACCCTCGCGTCTCGCATCTCTCATCGGACAACCTTCACCTCATCTTTCACCCTGCTCGACGTGCAAACCAATGATAATGATCTGCTCGGTCAGGTGCGGGTTCTCGCCACCCGCGGCCACCTCCAGGAAGCGCTCTCACTCTTCTACTCCCTTCATCCCCCACCACATCACTGCCCCCAAACCTACGCCACTCTCTTCCACGCCTGTGCCCGCCACCGCCGCCTCCAAGACGGCCTCTCTTTGCACCGTTACATGATCGCCCACAACCCCATCCACCCACCCGACCTCTTTGTCACCAACCACCTCATCAATATGTATGCCAAATTTGGTCACTTGGACTACGCCCACCAACTGTTTGATGGAATGCCCAGTAGAAACCTTGTCTCTTGGACCGCTCTCATTTCCGGGTACGCCCTATGCGGGCAACCCCAAAGGTGTTTGAGTTTATTTGCTGGCATGTTGGTTCATCACCGCCCCAACGAATTTGCTTTCACCAGCGTGCTCAGCTCACTCCCGGAGAGTGACGGTGTTTATGGTCAGCAGGTACATGCGCTTGCTTTGAAAATGTCTTTGGATGCTTGTGTTTACGTTGCAAATGCTCTTATTACTCTGTACGGCAAGATTTGTAATCCTGGAGGTGTTTATGATGCTAGTAAAGACGAGGCGTGGAATGTGTTTAAGAGCATGGAGTTCCGGAATCTTATATCTTGGAATTCAATGATTGCAGGGTACCGGTATCGCGGACTTGGGGCTCAAGCTATTCATCTTTTCATACAAATGTACCTTGACGGAATTGGGTTTGATCATGCTACACTTCTCAGCGTCCTCTCATCCTTATGCAGAAGCAACGGCCTTGATGATAATGTGGTTTCTAAGTTTTGTTTCCAATTGCACTGTCTCACGATCAGAACCGGGCTTATATTAAAAATTGAAGTTGCCACTGCGTTAGTTAAAGCTTATTCAGATGTTGGAGGAGATGTTGCGGACTGTTACAGGCTTTTCTTGGAGACAAGTTGTCATCGGGATATTGTTGCATGGACTGGCATCATAACAACTTTTGCAGAGCGAGACCCTGAAGAATCACTCTTCCTCTTTCGTCAATTGCGTCGTGAGAACTTAGTTCCCGACAGGTATACTTTCTCAATTGTTTTGAAAGCTTATGCAAGCCTTGCAACCGAGCGCCACACCTTGGCAGTTCATTCACAAGTGATCAAAGCTGGCTTTGAGTGTGACACAGTACTTGCAAATGCCTTGATCCATGCCTATGCTAGGTGTGGCTCCATTTCCTTGTCTAAGAAGGTGTTTGATGGAATTGAACTTCGTGATGTCATTTCGTGGAATACAATGATTAAGGCTTATGCCTTGTATGGACAAGCTACAGAGGCGTTGCATCTCTTCTCACGTATGAACGTCCAACCTGATCCTGCCACCTTTGTTTCTCTCCTCTGTGCTTGTAGCCATGCTGGACTGGTAGAAGAAGGAACTCGCATCTTTGACTCCATGATTGAGGTATATGGTATTGTACCTCAACTTGATCATTACGCCTGCATGGTTGATATTCTTGGTCGAGCTGGACAGATTGTTGAGGCTGAGGAGCTTCTAAGTCGAATGCCAATGGAGCCTGATTCTGTAGTCTGGAGTGCACTCCTCGGGTCATGTAGAAAGCATGGAAAAACACCGTTGGCCAAGTTAGCAGCAGATCGACTGAAAGAGCTAGCACCAGACAATTCATTAGGTTATGTACAAATGTCAAACATGTTTTGCTCTGACGGTAAATTTGGTGAAGCAGGCCTAATTAGGAAGGAAATGAAAGGGTCTAGAGTGAAAAAGGAACCTGGATTAAGCTGGATTGAGATTGGAAATCGGGTCCATGAGTTTTGCTCTGGAGGCCAACATCATCCAGAAAGGAACGCTATCTTCAGGAATTTAGAAGAACTGATTGTGCGGTTAAGAGAAATGGGTTATGTTCCAGACACAAGCTTGTCAGTGCATGATGTGGAAGAGGAACACAAAGAGGAGCAGTTGTACCATCATAGTGAAAAGCTAGCTTTAGTATTTGCTATTATGAATGAAGGTAGTTCAAATTGTGGTCGGACTGCTATAAAAATAATGAAGAACATAAGAATCTGCGTGGACTGCCATAACTTCATGAAGTTAGCATCAAATCTACTTCAGAAAGAGATTTTTGTGAGAGACTCAAACCGGTTCCACCACTTTAATGATGGAATATGCTCTTGCAATGACTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

758

Amino Acids

85.05

Weight (kDa)

6.68

Isoelectric Point (pI)

40.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	448 - 493	4.1e-07	PPR repeat family
E_motif	PF20431	567 - 625	1.5e-13	E motif
DYW_deaminase	PF14432	661 - 757	1.5e-35	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71420
fragaria_vesca	FvH4_2g00530 FvH4_2g00530 FvH4_2g00560 FvH4_2g00560 FvH4_2g00560 FvH4_2g00560
malus_domestica	MD05G1002500.v1.1
prunus_persica	Prupe.8G005700_v2.0.a1
pyrus_communis	pycom05g00240
rosa_chinensis	RchiOBHm_Chr6g0250571 RchiOBHm_Chr6g0250641 RchiOBHm_Chr6g0250661 RchiOBHm_Chr6g0250741 RchiOBHm_Chr6g0250751 RchiOBHm_Chr6g0250881 RchiOBHm_Chr6g0250901 RchiOBHm_Chr6g0250941 RchiOBHm_Chr6g0251021
rosa_laevigata	RLG00000001886 RLG00000015490 RLG00000015491 RLG00000015495 RLG00000015496 RLG00000015497 RLG00000015516 RLG00000015517 RLG00000015521 RLG00000015534
rosa_multiflora	Rmu_co8213794.1_g000001 Rmu_co8225142.1_g000001 Rmu_co8465043.1_g000001 Rmu_sc0001209.1_g000010 Rmu_sc0001209.1_g000028 Rmu_sc0001209.1_g000030 Rmu_sc0003527.1_g000008 Rmu_sc0026821.1_g000002 Rmu_sc0031313.1_g000001 Rmu_sc0037610.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217150
rosa_rugosa	Rorug05G0498000 Rorug05G0499000 Rorug05G0499100 Rorug05G0499400
rosa_samantha	Rh6AG006800 Rh6AG007100 Rh6BG003500 Rh6BG003800 Rh6DG005100
rosa_wichuraiana	Rw6G000790 Rw6G000840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	311
Acc36I	ACCTGC	1 cut(s)	553
Acc65I	GGTACC	1 cut(s)	750
AccB1I	GGYRCC	1 cut(s)	750
AccB7I	CCANNNNNTGG	1 cut(s)	1210
AccBSI	CCGCTC	1 cut(s)	416
AccI	GTMKAC	1 cut(s)	1154
AccII	CGCG	3 cut(s)	29, 135, 762
AccIII	TCCGGA	1 cut(s)	714
AclWI	GGATC	2 cut(s)	1270, 1436
AcoI	YGGCCR	2 cut(s)	136, 1704
AcsI	RAATTY	5 cut(s)	344, 503, 733, 1796, 1945
AcuI	CTGAAG	3 cut(s)	1116, 1924, 2177
AfaI	GTAC	9 cut(s)	431, 567, 635, 752, 803, 1260, 1545, 1764, 2048
AfeI	AGCGCT	1 cut(s)	153
AfiI	CCNNNNNNNGG	4 cut(s)	145, 441, 1210, 1290
AflIII	ACRYGT	1 cut(s)	1776
AgeI	ACCGGT	2 cut(s)	753, 2223
AgsI	TTSAA	6 cut(s)	583, 738, 953, 1174, 1337, 2103
AhdI	GACNNNNNGTC	1 cut(s)	1035
AjiI	CACGTC	1 cut(s)	82
AjnI	CCWGG	3 cut(s)	144, 652, 1855
AjuI	GAANNNNNNNTTGG	2 cut(s)	575, 607
AleI	CACNNNNGTG	2 cut(s)	225, 518
Alw21I	GWGCWC	3 cut(s)	19, 525, 1666
Alw26I	GTCTC	6 cut(s)	36, 409, 923, 1022, 1083, 2208
Alw44I	GTGCAC	1 cut(s)	1662
AlwI	GGATC	2 cut(s)	1270, 1436
Ama87I	CYCGRG	1 cut(s)	1670
Aor13HI	TCCGGA	1 cut(s)	714
Aor51HI	AGCGCT	1 cut(s)	153
AoxI	GGCC	6 cut(s)	136, 253, 871, 1704, 1810, 1909
ApaLI	GTGCAC	1 cut(s)	1662
ApeKI	GCWGC	1 cut(s)	1715
ApoI	RAATTY	5 cut(s)	344, 503, 733, 1796, 1945
ArsI	GACNNNNNNTTYG	2 cut(s)	1781, 1813
AsiGI	ACCGGT	2 cut(s)	753, 2223
Asp718I	GGTACC	1 cut(s)	750
AspLEI	GCGC	3 cut(s)	154, 574, 1203
AspS9I	GGNCC	2 cut(s)	411, 1888
AsuC2I	CCSGG	3 cut(s)	427, 536, 934
AsuHPI	GGTGA	7 cut(s)	13, 49, 61, 307, 482, 505, 1814
AsuNHI	GCTAGC	2 cut(s)	1735, 2065
AvaI	CYCGRG	1 cut(s)	1670
AvaII	GGWCC	2 cut(s)	411, 1888
BaeGI	GKGCMC	2 cut(s)	232, 1666
BaeI	ACNNNNGTAYC	2 cut(s)	785, 818
BalI	TGGCCA	1 cut(s)	1706
BanI	GGYRCC	1 cut(s)	750
BanII	GRGCYC	1 cut(s)	775
Bbv12I	GWGCWC	3 cut(s)	19, 525, 1666
BbvCI	CCTCAGC	1 cut(s)	1611
BbvI	GCAGC	1 cut(s)	1727
BccI	CCATC	5 cut(s)	299, 374, 1322, 2058, 2237
BceAI	ACGGC	3 cut(s)	268, 652, 886
BciT130I	CCWGG	3 cut(s)	146, 654, 1857
BclI	TGATCA	3 cut(s)	823, 1230, 1555
BcnI	CCSGG	3 cut(s)	427, 536, 934
BcoDI	GTCTC	6 cut(s)	36, 409, 923, 1022, 1083, 2208
BfaI	CTAG	5 cut(s)	672, 1290, 1736, 1839, 2066
BfmI	CTRYAG	2 cut(s)	1398, 1650
BfoI	RGCGCY	2 cut(s)	155, 1204
BfuAI	ACCTGC	1 cut(s)	553
BisI	GCNGC	3 cut(s)	136, 241, 1716
BlpI	GCTNAGC	1 cut(s)	524
BlsI	GCNGC	3 cut(s)	137, 242, 1717
BmcAI	AGTACT	1 cut(s)	1260
Bme1390I	CCNGG	6 cut(s)	146, 427, 536, 654, 934, 1857
Bme18I	GGWCC	2 cut(s)	411, 1888
BmeRI	GACNNNNNGTC	1 cut(s)	1035
BmeT110I	CYCGRG	1 cut(s)	1670
BmgBI	CACGTC	1 cut(s)	82
BmgT120I	GGNCC	2 cut(s)	411, 1888
BmiI	GGNNCC	6 cut(s)	752, 1300, 1641, 1854, 1889, 2228
BmrFI	CCNGG	6 cut(s)	146, 427, 536, 654, 934, 1857
BmrI	ACTGGG	1 cut(s)	384
BmsI	GCATC	7 cut(s)	45, 586, 658, 1074, 1420, 1518, 2189
BmtI	GCTAGC	2 cut(s)	1739, 2069
BmuI	ACTGGG	1 cut(s)	384
BplI	GAGNNNNNCTC	2 cut(s)	1886, 1918
BpmI	CTGGAG	4 cut(s)	128, 675, 1678, 1926
Bpu10I	CCTNAGC	1 cut(s)	1611
Bpu1102I	GCTNAGC	1 cut(s)	524
BpuEI	CTTGAG	1 cut(s)	759
BpuMI	CCSGG	3 cut(s)	427, 536, 934
BsaAI	YACGTR	1 cut(s)	1424
BsaBI	GATNNNNATC	1 cut(s)	99
BsaI	GGTCTC	1 cut(s)	1083
BsaJI	CCNNGG	3 cut(s)	133, 1209, 1669
BsaWI	WCCGGW	3 cut(s)	714, 753, 2223
BsaXI	ACNNNNNCTCC	4 cut(s)	984, 1014, 2030, 2060
Bsc4I	CCNNNNNNNGG	4 cut(s)	145, 441, 1210, 1290
Bse118I	RCCGGY	2 cut(s)	753, 2223
Bse1I	ACTGG	4 cut(s)	390, 1066, 1494, 2273
Bse3DI	GCAATG	1 cut(s)	2266
Bse8I	GATNNNNATC	1 cut(s)	99
BseAI	TCCGGA	1 cut(s)	714
BseBI	CCWGG	3 cut(s)	146, 654, 1857
BseDI	CCNNGG	3 cut(s)	133, 1209, 1669
BseGI	GGATG	5 cut(s)	175, 291, 601, 851, 1918
BseJI	GATNNNNATC	1 cut(s)	99
BseLI	CCNNNNNNNGG	4 cut(s)	145, 441, 1210, 1290
BseMI	GCAATG	1 cut(s)	2266
BseMII	CTCAG	3 cut(s)	538, 853, 1602
BseNI	ACTGG	4 cut(s)	390, 1066, 1494, 2273
BseRI	GAGGAG	5 cut(s)	1008, 1454, 1628, 1658, 2051
BseSI	GKGCMC	2 cut(s)	232, 1666
BseXI	GCAGC	1 cut(s)	1727
Bsh1236I	CGCG	3 cut(s)	29, 135, 762
BshFI	GGCC	6 cut(s)	138, 255, 873, 1706, 1812, 1911
BshNI	GGYRCC	1 cut(s)	750
BshTI	ACCGGT	2 cut(s)	753, 2223
BsiHKAI	GWGCWC	3 cut(s)	19, 525, 1666
BsiHKCI	CYCGRG	1 cut(s)	1670
BsiSI	CCGG	6 cut(s)	426, 536, 715, 754, 933, 2224
BslI	CCNNNNNNNGG	4 cut(s)	145, 441, 1210, 1290
BsmAI	GTCTC	6 cut(s)	36, 409, 923, 1022, 1083, 2208
BsmBI	CGTCTC	1 cut(s)	36
BsmI	GAATGC	2 cut(s)	390, 1635
BsnI	GGCC	6 cut(s)	138, 255, 873, 1706, 1812, 1911
Bso31I	GGTCTC	1 cut(s)	1083
BsoBI	CYCGRG	1 cut(s)	1670
Bsp1286I	GDGCHC	5 cut(s)	19, 232, 525, 775, 1666
Bsp13I	TCCGGA	1 cut(s)	714
Bsp1407I	TGTACA	1 cut(s)	1762
Bsp143I	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
Bsp1720I	GCTNAGC	1 cut(s)	524
BspANI	GGCC	6 cut(s)	138, 255, 873, 1706, 1812, 1911
BspCNI	CTCAG	3 cut(s)	537, 852, 1603
BspEI	TCCGGA	1 cut(s)	714
BspFNI	CGCG	3 cut(s)	29, 135, 762
BspHI	TCATGA	1 cut(s)	2169
BspLI	GGNNCC	6 cut(s)	752, 1300, 1641, 1854, 1889, 2228
BspMI	ACCTGC	1 cut(s)	553
BspOI	GCTAGC	2 cut(s)	1739, 2069
BspPI	GGATC	2 cut(s)	1270, 1436
BspT107I	GGYRCC	1 cut(s)	750
BspTNI	GGTCTC	1 cut(s)	1083
BsrBI	CCGCTC	1 cut(s)	416
BsrDI	GCAATG	1 cut(s)	2266
BsrFI	RCCGGY	2 cut(s)	753, 2223
BsrGI	TGTACA	1 cut(s)	1762
BsrI	ACTGG	4 cut(s)	390, 1066, 1494, 2273
BssAI	RCCGGY	2 cut(s)	753, 2223
BssECI	CCNNGG	3 cut(s)	133, 1209, 1669
BssMI	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
BssNAI	GTATAC	1 cut(s)	1155
BssT1I	CCWWGG	1 cut(s)	1209
Bst1107I	GTATAC	1 cut(s)	1155
Bst2UI	CCWGG	3 cut(s)	146, 654, 1857
Bst4CI	ACNGT	8 cut(s)	269, 375, 548, 917, 1010, 1258, 1701, 1793
Bst6I	CTCTTC	5 cut(s)	167, 221, 1112, 1421, 2016
BstAPI	GCANNNNNTGC	1 cut(s)	1190
BstAUI	TGTACA	1 cut(s)	1762
BstBAI	YACGTR	1 cut(s)	1424
BstDEI	CTNAG	7 cut(s)	524, 839, 894, 1138, 1314, 1611, 1622
BstDSI	CCRYGG	1 cut(s)	133
BstENI	CCTNNNNNAGG	1 cut(s)	1288
BstF5I	GGATG	5 cut(s)	175, 291, 601, 851, 1918
BstFNI	CGCG	3 cut(s)	29, 135, 762
BstH2I	RGCGCY	2 cut(s)	155, 1204
BstHHI	GCGC	3 cut(s)	154, 574, 1203
BstKTI	GATC	9 cut(s)	103, 279, 826, 927, 1233, 1278, 1444, 1558, 1723
BstMAI	GTCTC	6 cut(s)	36, 409, 923, 1022, 1083, 2208
BstMBI	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
BstMWI	GCNNNNNNNGC	9 cut(s)	82, 240, 525, 1190, 1403, 1639, 1712, 1908, 2157
BstNI	CCWGG	3 cut(s)	146, 654, 1857
BstNSI	RCATGY	3 cut(s)	481, 572, 1780
BstSCI	CCNGG	6 cut(s)	144, 425, 534, 652, 932, 1855
BstSFI	CTRYAG	2 cut(s)	1398, 1650
BstSLI	GKGCMC	2 cut(s)	232, 1666
BstUI	CGCG	3 cut(s)	29, 135, 762
BstV1I	GCAGC	1 cut(s)	1727
BstZ17I	GTATAC	1 cut(s)	1155
BsuRI	GGCC	6 cut(s)	138, 255, 873, 1706, 1812, 1911
BtgI	CCRYGG	1 cut(s)	133
BtrI	CACGTC	1 cut(s)	82
BtsCI	GGATG	5 cut(s)	175, 291, 601, 851, 1918
BtsI	GCAGTG	2 cut(s)	190, 960
BtsIMutI	CAGTG	4 cut(s)	190, 913, 960, 2013
BveI	ACCTGC	1 cut(s)	553
CciI	TCATGA	1 cut(s)	2169
CfoI	GCGC	3 cut(s)	154, 574, 1203
Cfr10I	RCCGGY	2 cut(s)	753, 2223
Cfr13I	GGNCC	2 cut(s)	411, 1888
Cfr42I	CCGCGG	1 cut(s)	136
CseI	GACGC	3 cut(s)	18, 832, 1115
Csp6I	GTAC	9 cut(s)	430, 566, 634, 751, 802, 1259, 1544, 1763, 2047
CspAI	ACCGGT	2 cut(s)	753, 2223
CviQI	GTAC	9 cut(s)	430, 566, 634, 751, 802, 1259, 1544, 1763, 2047
DdeI	CTNAG	7 cut(s)	524, 839, 894, 1138, 1314, 1611, 1622
DpnI	GATC	9 cut(s)	102, 278, 825, 926, 1232, 1277, 1443, 1557, 1722
DpnII	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
DrdI	GACNNNNNNGTC	1 cut(s)	311
DriI	GACNNNNNGTC	1 cut(s)	1035
DseDI	GACNNNNNNGTC	1 cut(s)	311
EaeI	YGGCCR	2 cut(s)	136, 1704
Eam1104I	CTCTTC	5 cut(s)	167, 221, 1112, 1421, 2016
Eam1105I	GACNNNNNGTC	1 cut(s)	1035
EarI	CTCTTC	5 cut(s)	167, 221, 1112, 1421, 2016
Eco130I	CCWWGG	1 cut(s)	1209
Eco147I	AGGCCT	1 cut(s)	1812
Eco24I	GRGCYC	1 cut(s)	775
Eco31I	GGTCTC	1 cut(s)	1083
Eco47I	GGWCC	2 cut(s)	411, 1888
Eco47III	AGCGCT	1 cut(s)	153
Eco57I	CTGAAG	3 cut(s)	1116, 1924, 2177
Eco88I	CYCGRG	1 cut(s)	1670
EcoNI	CCTNNNNNAGG	1 cut(s)	1288
EcoRI	GAATTC	1 cut(s)	733
EcoRII	CCWGG	3 cut(s)	144, 652, 1855
EcoT14I	CCWWGG	1 cut(s)	1209
EcoT38I	GRGCYC	1 cut(s)	775
ErhI	CCWWGG	1 cut(s)	1209
Esp3I	CGTCTC	1 cut(s)	36
FauI	CCCGC	4 cut(s)	111, 140, 239, 434
FbaI	TGATCA	3 cut(s)	823, 1230, 1555
FblI	GTMKAC	1 cut(s)	1154
Fnu4HI	GCNGC	3 cut(s)	136, 241, 1716
FokI	GGATG	5 cut(s)	162, 278, 608, 838, 1905
FriOI	GRGCYC	1 cut(s)	775
Fsp4HI	GCNGC	3 cut(s)	136, 241, 1716
FspBI	CTAG	5 cut(s)	672, 1290, 1736, 1839, 2066
GlaI	GCGC	3 cut(s)	153, 573, 1202
GluI	GCNGC	3 cut(s)	136, 241, 1716
GsuI	CTGGAG	4 cut(s)	128, 675, 1678, 1926
HaeII	RGCGCY	2 cut(s)	155, 1204
HaeIII	GGCC	6 cut(s)	138, 255, 873, 1706, 1812, 1911
HapII	CCGG	6 cut(s)	426, 536, 715, 754, 933, 2224
HgaI	GACGC	3 cut(s)	18, 832, 1115
HhaI	GCGC	3 cut(s)	154, 574, 1203
Hin6I	GCGC	3 cut(s)	152, 572, 1201
HinP1I	GCGC	3 cut(s)	152, 572, 1201
HindIII	AAGCTT	3 cut(s)	975, 1176, 1999
HinfI	GANTC	6 cut(s)	718, 1100, 1517, 1646, 2145, 2216
HpaII	CCGG	6 cut(s)	426, 536, 715, 754, 933, 2224
HphI	GGTGA	7 cut(s)	13, 49, 61, 307, 482, 505, 1814
Hpy166II	GTNNAC	4 cut(s)	607, 1155, 1664, 2155
Hpy188I	TCNGA	6 cut(s)	48, 929, 985, 1789, 2115, 2196
Hpy8I	GTNNAC	4 cut(s)	607, 1155, 1664, 2155
Hpy99I	CGWCG	2 cut(s)	83, 1131
HpyAV	CCTTC	6 cut(s)	64, 182, 1312, 1493, 1816, 2087
HpyCH4III	ACNGT	8 cut(s)	269, 375, 548, 917, 1010, 1258, 1701, 1793
HpyCH4IV	ACGT	4 cut(s)	81, 609, 1423, 1431
HpyF10VI	GCNNNNNNNGC	9 cut(s)	82, 240, 525, 1190, 1403, 1639, 1712, 1908, 2157
HpyF3I	CTNAG	7 cut(s)	524, 839, 894, 1138, 1314, 1611, 1622
HpySE526I	ACGT	4 cut(s)	81, 609, 1423, 1431
HspAI	GCGC	3 cut(s)	152, 572, 1201
Kpn2I	TCCGGA	1 cut(s)	714
KpnI	GGTACC	1 cut(s)	754
Ksp22I	TGATCA	3 cut(s)	823, 1230, 1555
KspI	CCGCGG	1 cut(s)	136
Kzo9I	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
LmnI	GCTCC	4 cut(s)	1304, 1615, 1639, 2038
Lsp1109I	GCAGC	1 cut(s)	1727
LweI	GCATC	7 cut(s)	45, 586, 658, 1074, 1420, 1518, 2189
MaeI	CTAG	5 cut(s)	672, 1290, 1736, 1839, 2066
MaeII	ACGT	4 cut(s)	81, 609, 1423, 1431
MaeIII	GTNAC	7 cut(s)	3, 269, 313, 350, 542, 1010, 1250
MalI	GATC	9 cut(s)	102, 278, 825, 926, 1232, 1277, 1443, 1557, 1722
MbiI	CCGCTC	1 cut(s)	416
MboI	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
MfeI	CAATTG	3 cut(s)	908, 1121, 1164
MhlI	GDGCHC	5 cut(s)	19, 232, 525, 775, 1666
MlsI	TGGCCA	1 cut(s)	1706
MluNI	TGGCCA	1 cut(s)	1706
MlyI	GAGTC	2 cut(s)	1511, 2210
MmeI	TCCRAC	3 cut(s)	970, 1459, 2093
Mox20I	TGGCCA	1 cut(s)	1706
MroI	TCCGGA	1 cut(s)	714
MscI	TGGCCA	1 cut(s)	1706
MseI	TTAA	7 cut(s)	699, 944, 972, 1371, 1862, 1970, 2238
MslI	CAYNNNNRTG	8 cut(s)	225, 332, 518, 797, 856, 1284, 2055, 2238
Msp20I	TGGCCA	1 cut(s)	1706
MspA1I	CMGCKG	1 cut(s)	135
MspI	CCGG	6 cut(s)	426, 536, 715, 754, 933, 2224
MspR9I	CCNGG	6 cut(s)	146, 427, 536, 654, 934, 1857
MunI	CAATTG	3 cut(s)	908, 1121, 1164
Mva1269I	GAATGC	2 cut(s)	390, 1635
MvaI	CCWGG	3 cut(s)	146, 654, 1857
MvnI	CGCG	3 cut(s)	29, 135, 762
MwoI	GCNNNNNNNGC	9 cut(s)	82, 240, 525, 1190, 1403, 1639, 1712, 1908, 2157
NciI	CCSGG	3 cut(s)	427, 536, 934
NdeII	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
NheI	GCTAGC	2 cut(s)	1735, 2065
NlaIV	GGNNCC	6 cut(s)	752, 1300, 1641, 1854, 1889, 2228
NmuCI	GTSAC	4 cut(s)	313, 350, 542, 1250
NspI	RCATGY	3 cut(s)	481, 572, 1780
OliI	CACNNNNGTG	2 cut(s)	225, 518
PagI	TCATGA	1 cut(s)	2169
PceI	AGGCCT	1 cut(s)	1812
PciI	ACATGT	1 cut(s)	1776
PcsI	WCGNNNNNNNCGW	1 cut(s)	1123
PctI	GAATGC	2 cut(s)	390, 1635
PfeI	GAWTC	4 cut(s)	718, 1100, 1646, 2145
PflMI	CCANNNNNTGG	1 cut(s)	1210
PfoI	TCCNGGA	3 cut(s)	144, 534, 652
PinAI	ACCGGT	2 cut(s)	753, 2223
PkrI	GCNGC	3 cut(s)	137, 242, 1717
PleI	GAGTC	2 cut(s)	1511, 2210
PpsI	GAGTC	2 cut(s)	1511, 2210
Ppu21I	YACGTR	1 cut(s)	1424
PscI	ACATGT	1 cut(s)	1776
Psp6I	CCWGG	3 cut(s)	144, 652, 1855
PspGI	CCWGG	3 cut(s)	144, 652, 1855
PspN4I	GGNNCC	6 cut(s)	752, 1300, 1641, 1854, 1889, 2228
PspPI	GGNCC	2 cut(s)	411, 1888
RsaI	GTAC	9 cut(s)	431, 567, 635, 752, 803, 1260, 1545, 1764, 2048
RsaNI	GTAC	9 cut(s)	430, 566, 634, 751, 802, 1259, 1544, 1763, 2047
RseI	CAYNNNNRTG	8 cut(s)	225, 332, 518, 797, 856, 1284, 2055, 2238
SacII	CCGCGG	1 cut(s)	136
SaqAI	TTAA	7 cut(s)	699, 944, 972, 1371, 1862, 1970, 2238
SatI	GCNGC	3 cut(s)	136, 241, 1716
Sau3AI	GATC	9 cut(s)	100, 276, 823, 924, 1230, 1275, 1441, 1555, 1720
Sau96I	GGNCC	2 cut(s)	411, 1888
ScaI	AGTACT	1 cut(s)	1260
SchI	GAGTC	2 cut(s)	1511, 2210
ScrFI	CCNGG	6 cut(s)	146, 427, 536, 654, 934, 1857
SduI	GDGCHC	5 cut(s)	19, 232, 525, 775, 1666
SfaNI	GCATC	7 cut(s)	45, 586, 658, 1074, 1420, 1518, 2189
SfcI	CTRYAG	2 cut(s)	1398, 1650
Sfr303I	CCGCGG	1 cut(s)	136
SgrBI	CCGCGG	1 cut(s)	136
SinI	GGWCC	2 cut(s)	411, 1888
SmiMI	CAYNNNNRTG	8 cut(s)	225, 332, 518, 797, 856, 1284, 2055, 2238
SmlI	CTYRAG	1 cut(s)	774
SmoI	CTYRAG	1 cut(s)	774
SseBI	AGGCCT	1 cut(s)	1812
SspMI	CTAG	5 cut(s)	672, 1290, 1736, 1839, 2066
StuI	AGGCCT	1 cut(s)	1812
StyD4I	CCNGG	6 cut(s)	144, 425, 534, 652, 932, 1855
StyI	CCWWGG	1 cut(s)	1209
TaaI	ACNGT	8 cut(s)	269, 375, 548, 917, 1010, 1258, 1701, 1793
TaiI	ACGT	4 cut(s)	84, 612, 1426, 1434
TaqI	TCGA	4 cut(s)	78, 1588, 1627, 1723
TaqII	GACCGA	1 cut(s)	98
TatI	WGTACW	2 cut(s)	1258, 1762
TauI	GCSGC	2 cut(s)	138, 243
TfiI	GAWTC	4 cut(s)	718, 1100, 1646, 2145
Tru1I	TTAA	7 cut(s)	699, 944, 972, 1371, 1862, 1970, 2238
Tru9I	TTAA	7 cut(s)	699, 944, 972, 1371, 1862, 1970, 2238
TscAI	CASTG	4 cut(s)	197, 920, 967, 2013
TseFI	GTSAC	4 cut(s)	313, 350, 542, 1250
TseI	GCWGC	1 cut(s)	1715
Tsp45I	GTSAC	4 cut(s)	313, 350, 542, 1250
TspGWI	ACGGA	1 cut(s)	825
TspRI	CASTG	4 cut(s)	197, 920, 967, 2013
Van91I	CCANNNNNTGG	1 cut(s)	1210
VneI	GTGCAC	1 cut(s)	1662
VpaK11BI	GGWCC	2 cut(s)	411, 1888
XagI	CCTNNNNNAGG	1 cut(s)	1288
XapI	RAATTY	5 cut(s)	344, 503, 733, 1796, 1945
XbaI	TCTAGA	1 cut(s)	1838
XceI	RCATGY	3 cut(s)	481, 572, 1780
XcmI	CCANNNNNNNNNTGG	2 cut(s)	377, 2240
XmiI	GTMKAC	1 cut(s)	1154
XspI	CTAG	5 cut(s)	672, 1290, 1736, 1839, 2066
ZrmI	AGTACT	1 cut(s)	1260

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.