Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0001522

Physical Location & Seq

Genomic Coordinates Reverse (-)

559030 .. 561796

Gene Structure 2767 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG003800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2265 bp

ATGCTACGCCCATGTGGTCGCGCGTGTCATAGAACCTTCACCTCAATCAACCATCCGGTTCTTCCCACACCTCCCCGCCTCAACCTGCAAACAAATAACCTTCTCGGCGAAGTAGGGGCCCTGGCCACGCGCGGCCAACTCAACGAAGCACTCTCACTCTTCTACGCTCTTCAAACTCACTGCAACCAAACCTACGCCACTCTCTTCCACGCATGTGCTCGCCACCACTCCCTCCAACAAGGCCTCTCTCTGCACCATTACATGCTTGCCCACAACCCCATCACTCCTCATTCCGACCTCTTTGTCTCCAATCACCTCATCAACATGTACGCCAAATTTGGCTACTTAGACCATGCCCGCCATCTGTTCGATGAAATGCCCAGAAGAAACCTCGTCACCTGGACCGCTCTCATTTCCGGGTACGCACAGCGGGGTCGAGCTGACAATTGTTTTCGTTTATTTTCTGCCATGTTGGGGCATTACCTCCCGAATGAGTTTGCATTTGCCAGCGTGCTAAGTTCGTGTGCCGATAGCGATGGTGGATATGGTCGGCAGGTACACGCCCTCGCTTTGAAAATGTCTTTTGATGCTTGCCTTTATGTTGCGAATGCTCTCATTACCATGTATAGCAAGGTGTGTGATCATGGAGGTGTGTTTGATGTTAGTAGAGACGAGGCATGCAAGGTGTTTAGAAGCATGGAATCCCGGAATCTTATATCGTGGAATTCCATGATTGCAGGGTTTCAGTGTCGTGGACGTGGGGCTCAAGCTATTGATCTGTTTGTACAAATGCACCTTGATGGACTCGAGTTTGACCGTGCCACGCTTCTCAGCGTCTTATCTTCCTTGAATGGAGTAAATGACATTGATGATGTTGTGGTTACTAAGTTTTGTTATCAATTGCATTGTCTGGTTATCAAAACCGGGTTTATATTAGGAATTGAAGTGGTCACTGCGCTAGTTAAAGCTTATTCAGATCTTGGAGGGGATGCTGCTGACTGTTACAGGCTCTTCTCTGAGACAAGCTGTCATCGGGATATTGTTGCATGGACGGGCATTATGACAATATTTTCCGAGCGGGACCCTGAAGAAGCACTCTCCCTTTTTCGTCAATTACGCCGGGAGAATTTAGCTCCAGACAGGTATACCTTCTCCATTGTTTTAAAAGCCTACGCAAGCCTTGCAACCGAGCGCCGTACCTTGGCAGTTCATTCTCAAGTTATCAAAGGCGGGTTTGGGGGTGACACAGTCCTTGCAAACGCCTTGATCCATGCATATGCTCGGTGCGGCTCCATTTCCTTGTCTAAGCAAGTTTTTGATGGAATTGAGTTTCGTGATGTTGTTTCTTGGAACACAATGCTCAAGGCTTATGCCTTGTATGGACAAGCTGCAGAGGCACTGCAGCTTTTTTCACAAATGGACGTTAAACCTGATTCTGCTACATTTGTTTCTCTCCTCTGTGCTTGTAGTCATGCTGGACTGGTAGAAGAAGGAACCAAAATCTTTGACTCCATGCTTGAGAGATATGGCATTGTACCTCTATGTGATCATTATGCCTGCATGGTTGACATTCTTGGTCGAGCTGGACGAGTTGGTGAGGCTGAGAAGCTTGTAAGTAGAATGCCAATGGAGCCCGATTCTGTTGTTTGGAGTGCACTCCTTGGATCATGTAGGAAGCATGGAAATACACAGTTGGCTAAGTTAGCAGCTGATAGACTGAAGGAGTTGGCTCCAGAGGGTTCATTAGTTTATGTACAAATGTCAAATATATATAGCTCTGATGGTAACTTTGGTGAAGCGGGCCTGATTAGGAAGGAAATGAAAGGGTCTCGAGTGAAAAAGGAACCTGGATTAAGCTGGATTGAGATCAGAAATCAGGTGCATGAGTTCTCCTCTGGAGGCCGACGCCATCCAGAAAGGAATCTTATAAGCAGAAAACTAAAAGAACTGATTGGACGGTTAAGGGAAATTGGTTATGTTCCAGATACAAGCTCATCCTTGCATGATGTGGAAGAGGAGCACAAAGAGGAGCAATTGTACCATCATAGTGAGAAGCTAGCTTTAGTGTTTGCCATTATGAATGAAAGCGGTTTACATTGCGGTAGGACTGGTATAAAAATAATGAAGAATATCAGAGTATGCGTGGATTGCCATAACTTCATGAAGTTAGCATCAAATCTACTTCAAAAGGATATTGTTTTGAGAGACTCAAACCGTTTCCACCACTTTGAAGATGGCATATGTTCTTGCAATGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

754

Amino Acids

84.33

Weight (kDa)

7.04

Isoelectric Point (pI)

44.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	130 - 176	3.8e-06	PPR repeat family
PPR_2	PF13041	346 - 392	7.3e-08	PPR repeat family
PPR_2	PF13041	445 - 490	8.9e-09	PPR repeat family
E_motif	PF20431	560 - 622	6.9e-15	E motif
DYW_deaminase	PF14432	658 - 754	1.4e-35	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71420
fragaria_vesca	FvH4_2g00530 FvH4_2g00530 FvH4_2g00560 FvH4_2g00560 FvH4_2g00560 FvH4_2g00560
malus_domestica	MD05G1002500.v1.1
prunus_persica	Prupe.8G005700_v2.0.a1
pyrus_communis	pycom05g00240
rosa_chinensis	RchiOBHm_Chr6g0250571 RchiOBHm_Chr6g0250641 RchiOBHm_Chr6g0250661 RchiOBHm_Chr6g0250741 RchiOBHm_Chr6g0250751 RchiOBHm_Chr6g0250881 RchiOBHm_Chr6g0250901 RchiOBHm_Chr6g0250941 RchiOBHm_Chr6g0251021
rosa_laevigata	RLG00000001886 RLG00000015490 RLG00000015491 RLG00000015495 RLG00000015496 RLG00000015497 RLG00000015516 RLG00000015517 RLG00000015521 RLG00000015534
rosa_multiflora	Rmu_co8213794.1_g000001 Rmu_co8225142.1_g000001 Rmu_co8465043.1_g000001 Rmu_sc0001209.1_g000010 Rmu_sc0001209.1_g000028 Rmu_sc0001209.1_g000030 Rmu_sc0003527.1_g000008 Rmu_sc0026821.1_g000002 Rmu_sc0031313.1_g000001 Rmu_sc0037610.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217150
rosa_rugosa	Rorug05G0498000 Rorug05G0499000 Rorug05G0499100 Rorug05G0499400
rosa_samantha	Rh6AG006800 Rh6AG007100 Rh6BG003500 Rh6BG003800 Rh6DG005100
rosa_wichuraiana	Rw6G000790 Rw6G000840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1928
AasI	GACNNNNNNGTC	1 cut(s)	302
Acc36I	ACCTGC	2 cut(s)	93, 544
AccBSI	CCGCTC	2 cut(s)	407, 1078
AccI	GTMKAC	1 cut(s)	1145
AccII	CGCG	4 cut(s)	21, 23, 130, 132
AclWI	GGATC	2 cut(s)	1261, 1672
AcoI	YGGCCR	2 cut(s)	123, 133
AcsI	RAATTY	3 cut(s)	335, 724, 1126
AcuI	CTGAAG	2 cut(s)	1107, 1739
AcyI	GRCGYC	1 cut(s)	1906
AfaI	GTAC	8 cut(s)	329, 422, 558, 786, 1198, 1536, 1755, 2039
AfiI	CCNNNNNNNGG	2 cut(s)	474, 1201
AflIII	ACRYGT	1 cut(s)	324
AgsI	TTSAA	6 cut(s)	173, 574, 850, 944, 2186, 2231
AhdI	GACNNNNNGTC	1 cut(s)	1026
AjiI	CACGTC	1 cut(s)	758
AjnI	CCWGG	3 cut(s)	120, 398, 1846
AleI	CACNNNNGTG	1 cut(s)	213
Alw21I	GWGCWC	3 cut(s)	220, 1657, 2022
Alw26I	GTCTC	5 cut(s)	310, 663, 1013, 1833, 2199
Alw44I	GTGCAC	1 cut(s)	1653
AlwI	GGATC	2 cut(s)	1261, 1672
Ama87I	CYCGRG	2 cut(s)	806, 1830
AoxI	GGCC	6 cut(s)	117, 123, 133, 241, 1801, 1900
ApaI	GGGCCC	1 cut(s)	121
ApaLI	GTGCAC	1 cut(s)	1653
ApeKI	GCWGC	4 cut(s)	992, 1388, 1402, 1706
ApoI	RAATTY	3 cut(s)	335, 724, 1126
AspLEI	GCGC	4 cut(s)	23, 132, 958, 1194
AspS9I	GGNCC	5 cut(s)	117, 118, 402, 1081, 1801
AsuC2I	CCSGG	4 cut(s)	418, 706, 925, 1121
AsuHPI	GGTGA	6 cut(s)	31, 305, 388, 1253, 1607, 1805
AsuNHI	GCTAGC	1 cut(s)	2056
AvaI	CYCGRG	2 cut(s)	806, 1830
AvaII	GGWCC	2 cut(s)	402, 1081
BaeGI	GKGCMC	2 cut(s)	121, 1657
BalI	TGGCCA	1 cut(s)	125
BanII	GRGCYC	3 cut(s)	121, 766, 1635
Bbv12I	GWGCWC	3 cut(s)	220, 1657, 2022
BbvI	GCAGC	4 cut(s)	979, 1375, 1414, 1718
BceAI	ACGGC	1 cut(s)	1179
BciT130I	CCWGG	3 cut(s)	122, 400, 1848
BclI	TGATCA	2 cut(s)	640, 1546
BcnI	CCSGG	4 cut(s)	418, 706, 925, 1121
BcoDI	GTCTC	5 cut(s)	310, 663, 1013, 1833, 2199
BfaI	CTAG	2 cut(s)	959, 2057
BfmI	CTRYAG	2 cut(s)	1389, 1400
BfoI	RGCGCY	1 cut(s)	1195
BfuAI	ACCTGC	2 cut(s)	93, 544
BglII	AGATCT	1 cut(s)	976
BisI	GCNGC	6 cut(s)	133, 993, 1288, 1389, 1403, 1707
BlsI	GCNGC	6 cut(s)	134, 994, 1289, 1390, 1404, 1708
Bme1390I	CCNGG	7 cut(s)	122, 400, 418, 706, 925, 1121, 1848
Bme18I	GGWCC	2 cut(s)	402, 1081
BmeRI	GACNNNNNGTC	1 cut(s)	1026
BmeT110I	CYCGRG	2 cut(s)	806, 1830
BmgBI	CACGTC	1 cut(s)	758
BmgT120I	GGNCC	5 cut(s)	117, 118, 402, 1081, 1801
BmiI	GGNNCC	9 cut(s)	118, 119, 1082, 1083, 1291, 1495, 1632, 1731, 1845
BmrFI	CCNGG	7 cut(s)	122, 400, 418, 706, 925, 1121, 1848
BmsI	GCATC	3 cut(s)	577, 979, 2180
BmtI	GCTAGC	1 cut(s)	2060
BplI	GAGNNNNNCTC	2 cut(s)	1877, 1909
BpmI	CTGGAG	3 cut(s)	1119, 1716, 1917
BpuEI	CTTGAG	4 cut(s)	750, 1200, 1346, 1538
BpuMI	CCSGG	4 cut(s)	418, 706, 925, 1121
BsaBI	GATNNNNATC	1 cut(s)	1865
BsaHI	GRCGYC	1 cut(s)	1906
BsaI	GGTCTC	1 cut(s)	1833
BsaJI	CCNNGG	3 cut(s)	120, 1200, 1660
BsaWI	WCCGGW	1 cut(s)	55
BsaXI	ACNNNNNCTCC	2 cut(s)	2021, 2051
Bsc4I	CCNNNNNNNGG	2 cut(s)	474, 1201
Bse1I	ACTGG	3 cut(s)	1485, 2113, 2264
Bse3DI	GCAATG	2 cut(s)	2095, 2257
Bse8I	GATNNNNATC	1 cut(s)	1865
BseBI	CCWGG	3 cut(s)	122, 400, 1848
BseDI	CCNNGG	3 cut(s)	120, 1200, 1660
BseGI	GGATG	4 cut(s)	52, 994, 1909, 1994
BseJI	GATNNNNATC	1 cut(s)	1865
BseLI	CCNNNNNNNGG	2 cut(s)	474, 1201
BseMI	GCAATG	2 cut(s)	2095, 2257
BseMII	CTCAG	3 cut(s)	844, 1008, 1593
BseNI	ACTGG	3 cut(s)	1485, 2113, 2264
BseRI	GAGGAG	5 cut(s)	276, 1445, 1882, 2030, 2042
BseSI	GKGCMC	2 cut(s)	121, 1657
BseXI	GCAGC	4 cut(s)	979, 1375, 1414, 1718
BsgI	GTGCAG	1 cut(s)	236
Bsh1236I	CGCG	4 cut(s)	21, 23, 130, 132
BshFI	GGCC	6 cut(s)	119, 125, 135, 243, 1803, 1902
BsiHKAI	GWGCWC	3 cut(s)	220, 1657, 2022
BsiHKCI	CYCGRG	2 cut(s)	806, 1830
BsiSI	CCGG	5 cut(s)	56, 417, 706, 924, 1120
BslFI	GGGAC	1 cut(s)	1094
BslI	CCNNNNNNNGG	2 cut(s)	474, 1201
BsmAI	GTCTC	5 cut(s)	310, 663, 1013, 1833, 2199
BsmBI	CGTCTC	1 cut(s)	663
BsmFI	GGGAC	1 cut(s)	1094
BsmI	GAATGC	2 cut(s)	613, 1626
BsnI	GGCC	6 cut(s)	119, 125, 135, 243, 1803, 1902
Bso31I	GGTCTC	1 cut(s)	1833
BsoBI	CYCGRG	2 cut(s)	806, 1830
Bsp120I	GGGCCC	1 cut(s)	117
Bsp1286I	GDGCHC	6 cut(s)	121, 220, 766, 1635, 1657, 2022
Bsp1407I	TGTACA	2 cut(s)	784, 1753
Bsp143I	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
BspANI	GGCC	6 cut(s)	119, 125, 135, 243, 1803, 1902
BspCNI	CTCAG	3 cut(s)	843, 1009, 1594
BspFNI	CGCG	4 cut(s)	21, 23, 130, 132
BspHI	TCATGA	1 cut(s)	2160
BspLI	GGNNCC	9 cut(s)	118, 119, 1082, 1083, 1291, 1495, 1632, 1731, 1845
BspMAI	CTGCAG	2 cut(s)	1393, 1404
BspMI	ACCTGC	2 cut(s)	93, 544
BspOI	GCTAGC	1 cut(s)	2060
BspPI	GGATC	2 cut(s)	1261, 1672
BspQI	GCTCTTC	2 cut(s)	174, 1016
BspTNI	GGTCTC	1 cut(s)	1833
BsrBI	CCGCTC	2 cut(s)	407, 1078
BsrDI	GCAATG	2 cut(s)	2095, 2257
BsrGI	TGTACA	2 cut(s)	784, 1753
BsrI	ACTGG	3 cut(s)	1485, 2113, 2264
BssECI	CCNNGG	3 cut(s)	120, 1200, 1660
BssMI	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
BssNAI	GTATAC	1 cut(s)	1146
BssNI	GRCGYC	1 cut(s)	1906
BssT1I	CCWWGG	2 cut(s)	1200, 1660
Bst1107I	GTATAC	1 cut(s)	1146
Bst2UI	CCWGG	3 cut(s)	122, 400, 1848
Bst4CI	ACNGT	6 cut(s)	818, 1001, 1249, 1692, 1959, 2216
Bst6I	CTCTTC	5 cut(s)	164, 174, 209, 1016, 2007
BstACI	GRCGYC	1 cut(s)	1906
BstAPI	GCANNNNNTGC	1 cut(s)	1181
BstAUI	TGTACA	2 cut(s)	784, 1753
BstDEI	CTNAG	8 cut(s)	346, 515, 830, 885, 1017, 1305, 1602, 1698
BstF5I	GGATG	4 cut(s)	52, 994, 1909, 1994
BstFNI	CGCG	4 cut(s)	21, 23, 130, 132
BstH2I	RGCGCY	1 cut(s)	1195
BstHHI	GCGC	4 cut(s)	23, 132, 958, 1194
BstKTI	GATC	7 cut(s)	643, 778, 979, 1269, 1549, 1667, 1869
BstMAI	GTCTC	5 cut(s)	310, 663, 1013, 1833, 2199
BstMBI	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
BstMWI	GCNNNNNNNGC	5 cut(s)	1181, 1394, 1630, 1703, 2148
BstNI	CCWGG	3 cut(s)	122, 400, 1848
BstNSI	RCATGY	4 cut(s)	216, 265, 328, 681
BstSCI	CCNGG	7 cut(s)	120, 398, 416, 704, 923, 1119, 1846
BstSFI	CTRYAG	2 cut(s)	1389, 1400
BstSLI	GKGCMC	2 cut(s)	121, 1657
BstUI	CGCG	4 cut(s)	21, 23, 130, 132
BstV1I	GCAGC	4 cut(s)	979, 1375, 1414, 1718
BstX2I	RGATCY	1 cut(s)	976
BstYI	RGATCY	1 cut(s)	976
BstZ17I	GTATAC	1 cut(s)	1146
BsuRI	GGCC	6 cut(s)	119, 125, 135, 243, 1803, 1902
BtgZI	GCGATG	1 cut(s)	549
BtrI	CACGTC	1 cut(s)	758
BtsCI	GGATG	4 cut(s)	52, 994, 1909, 1994
BtsI	GCAGTG	3 cut(s)	178, 951, 1397
BtsIMutI	CAGTG	4 cut(s)	178, 752, 951, 1397
BveI	ACCTGC	2 cut(s)	93, 544
CciI	TCATGA	1 cut(s)	2160
CfoI	GCGC	4 cut(s)	23, 132, 958, 1194
Cfr13I	GGNCC	5 cut(s)	117, 118, 402, 1081, 1801
CseI	GACGC	2 cut(s)	823, 1914
Csp6I	GTAC	8 cut(s)	328, 421, 557, 785, 1197, 1535, 1754, 2038
CviQI	GTAC	8 cut(s)	328, 421, 557, 785, 1197, 1535, 1754, 2038
DdeI	CTNAG	8 cut(s)	346, 515, 830, 885, 1017, 1305, 1602, 1698
DpnI	GATC	7 cut(s)	642, 777, 978, 1268, 1548, 1666, 1868
DpnII	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
DraI	TTTAAA	1 cut(s)	1164
DrdI	GACNNNNNNGTC	1 cut(s)	302
DriI	GACNNNNNGTC	1 cut(s)	1026
DseDI	GACNNNNNNGTC	1 cut(s)	302
EaeI	YGGCCR	2 cut(s)	123, 133
Eam1104I	CTCTTC	5 cut(s)	164, 174, 209, 1016, 2007
Eam1105I	GACNNNNNGTC	1 cut(s)	1026
EarI	CTCTTC	5 cut(s)	164, 174, 209, 1016, 2007
Eco130I	CCWWGG	2 cut(s)	1200, 1660
Eco147I	AGGCCT	1 cut(s)	243
Eco24I	GRGCYC	3 cut(s)	121, 766, 1635
Eco31I	GGTCTC	1 cut(s)	1833
Eco47I	GGWCC	2 cut(s)	402, 1081
Eco57I	CTGAAG	2 cut(s)	1107, 1739
Eco88I	CYCGRG	2 cut(s)	806, 1830
EcoO109I	RGGNCCY	3 cut(s)	117, 118, 1081
EcoRI	GAATTC	1 cut(s)	724
EcoRII	CCWGG	3 cut(s)	120, 398, 1846
EcoT14I	CCWWGG	2 cut(s)	1200, 1660
EcoT22I	ATGCAT	1 cut(s)	1276
EcoT38I	GRGCYC	3 cut(s)	121, 766, 1635
ErhI	CCWWGG	2 cut(s)	1200, 1660
Esp3I	CGTCTC	1 cut(s)	663
FaqI	GGGAC	1 cut(s)	1094
FauI	CCCGC	6 cut(s)	83, 365, 423, 1071, 1223, 1792
FauNDI	CATATG	2 cut(s)	1276, 2240
FbaI	TGATCA	2 cut(s)	640, 1546
FblI	GTMKAC	1 cut(s)	1145
Fnu4HI	GCNGC	6 cut(s)	133, 993, 1288, 1389, 1403, 1707
FokI	GGATG	4 cut(s)	39, 1001, 1896, 1981
FriOI	GRGCYC	3 cut(s)	121, 766, 1635
Fsp4HI	GCNGC	6 cut(s)	133, 993, 1288, 1389, 1403, 1707
FspBI	CTAG	2 cut(s)	959, 2057
GlaI	GCGC	4 cut(s)	22, 131, 957, 1193
GluI	GCNGC	6 cut(s)	133, 993, 1288, 1389, 1403, 1707
GsuI	CTGGAG	3 cut(s)	1119, 1716, 1917
HaeII	RGCGCY	1 cut(s)	1195
HaeIII	GGCC	6 cut(s)	119, 125, 135, 243, 1803, 1902
HapII	CCGG	5 cut(s)	56, 417, 706, 924, 1120
HgaI	GACGC	2 cut(s)	823, 1914
HhaI	GCGC	4 cut(s)	23, 132, 958, 1194
Hin1I	GRCGYC	1 cut(s)	1906
Hin6I	GCGC	4 cut(s)	21, 130, 956, 1192
HinP1I	GCGC	4 cut(s)	21, 130, 956, 1192
HincII	GTYRAC	1 cut(s)	1567
HindII	GTYRAC	1 cut(s)	1567
HindIII	AAGCTT	2 cut(s)	966, 1607
HinfI	GANTC	8 cut(s)	701, 709, 804, 1433, 1508, 1637, 1921, 2207
HpaII	CCGG	5 cut(s)	56, 417, 706, 924, 1120
HphI	GGTGA	6 cut(s)	31, 305, 388, 1253, 1607, 1805
Hpy166II	GTNNAC	6 cut(s)	559, 755, 1146, 1567, 1655, 2093
Hpy188I	TCNGA	7 cut(s)	295, 976, 1018, 1075, 1780, 1871, 2135
Hpy8I	GTNNAC	6 cut(s)	559, 755, 1146, 1567, 1655, 2093
Hpy99I	CGWCG	1 cut(s)	1908
HpyAV	CCTTC	6 cut(s)	46, 110, 1159, 1484, 1714, 1807
HpyCH4III	ACNGT	6 cut(s)	818, 1001, 1249, 1692, 1959, 2216
HpyCH4IV	ACGT	2 cut(s)	757, 1422
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1181, 1394, 1630, 1703, 2148
HpyF3I	CTNAG	8 cut(s)	346, 515, 830, 885, 1017, 1305, 1602, 1698
HpySE526I	ACGT	2 cut(s)	757, 1422
Hsp92I	GRCGYC	1 cut(s)	1906
HspAI	GCGC	4 cut(s)	21, 130, 956, 1192
KflI	GGGWCCC	1 cut(s)	1081
Ksp22I	TGATCA	2 cut(s)	640, 1546
Kzo9I	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
LguI	GCTCTTC	2 cut(s)	174, 1016
LmnI	GCTCC	6 cut(s)	1138, 1295, 1630, 1735, 2017, 2029
Lsp1109I	GCAGC	4 cut(s)	979, 1375, 1414, 1718
LweI	GCATC	3 cut(s)	577, 979, 2180
MaeI	CTAG	2 cut(s)	959, 2057
MaeII	ACGT	2 cut(s)	757, 1422
MaeIII	GTNAC	6 cut(s)	394, 880, 949, 1001, 1241, 1784
MalI	GATC	7 cut(s)	642, 777, 978, 1268, 1548, 1666, 1868
MbiI	CCGCTC	2 cut(s)	407, 1078
MboI	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
MfeI	CAATTG	3 cut(s)	445, 899, 2033
MflI	RGATCY	1 cut(s)	976
MhlI	GDGCHC	6 cut(s)	121, 220, 766, 1635, 1657, 2022
MlsI	TGGCCA	1 cut(s)	125
MluNI	TGGCCA	1 cut(s)	125
MlyI	GAGTC	3 cut(s)	798, 1502, 2201
MmeI	TCCRAC	2 cut(s)	259, 318
Mox20I	TGGCCA	1 cut(s)	125
Mph1103I	ATGCAT	1 cut(s)	1276
MscI	TGGCCA	1 cut(s)	125
MseI	TTAA	5 cut(s)	963, 1163, 1425, 1853, 1961
MslI	CAYNNNNRTG	7 cut(s)	213, 323, 620, 648, 798, 1275, 2046
Msp20I	TGGCCA	1 cut(s)	125
MspA1I	CMGCKG	2 cut(s)	430, 1709
MspI	CCGG	5 cut(s)	56, 417, 706, 924, 1120
MspR9I	CCNGG	7 cut(s)	122, 400, 418, 706, 925, 1121, 1848
MunI	CAATTG	3 cut(s)	445, 899, 2033
Mva1269I	GAATGC	2 cut(s)	613, 1626
MvaI	CCWGG	3 cut(s)	122, 400, 1848
MvnI	CGCG	4 cut(s)	21, 23, 130, 132
MwoI	GCNNNNNNNGC	5 cut(s)	1181, 1394, 1630, 1703, 2148
NciI	CCSGG	4 cut(s)	418, 706, 925, 1121
NdeI	CATATG	2 cut(s)	1276, 2240
NdeII	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
NheI	GCTAGC	1 cut(s)	2056
NlaIV	GGNNCC	9 cut(s)	118, 119, 1082, 1083, 1291, 1495, 1632, 1731, 1845
NmeAIII	GCCGAG	1 cut(s)	84
NmuCI	GTSAC	3 cut(s)	394, 949, 1241
NsiI	ATGCAT	1 cut(s)	1276
NspI	RCATGY	4 cut(s)	216, 265, 328, 681
OliI	CACNNNNGTG	1 cut(s)	213
PaeI	GCATGC	1 cut(s)	681
PaeR7I	CTCGAG	2 cut(s)	806, 1830
PagI	TCATGA	1 cut(s)	2160
PceI	AGGCCT	1 cut(s)	243
PciI	ACATGT	1 cut(s)	324
PciSI	GCTCTTC	2 cut(s)	174, 1016
PcsI	WCGNNNNNNNCGW	1 cut(s)	1585
PctI	GAATGC	2 cut(s)	613, 1626
PfeI	GAWTC	5 cut(s)	701, 709, 1433, 1637, 1921
PflFI	GACNNNGTC	1 cut(s)	1247
PfoI	TCCNGGA	1 cut(s)	704
PkrI	GCNGC	6 cut(s)	134, 994, 1289, 1390, 1404, 1708
PleI	GAGTC	3 cut(s)	798, 1502, 2201
PpsI	GAGTC	3 cut(s)	798, 1502, 2201
PpuMI	RGGWCCY	1 cut(s)	1081
PscI	ACATGT	1 cut(s)	324
PsiI	TTATAA	1 cut(s)	1928
Psp5II	RGGWCCY	1 cut(s)	1081
Psp6I	CCWGG	3 cut(s)	120, 398, 1846
PspGI	CCWGG	3 cut(s)	120, 398, 1846
PspN4I	GGNNCC	9 cut(s)	118, 119, 1082, 1083, 1291, 1495, 1632, 1731, 1845
PspOMI	GGGCCC	1 cut(s)	117
PspPI	GGNCC	5 cut(s)	117, 118, 402, 1081, 1801
PspPPI	RGGWCCY	1 cut(s)	1081
PspXI	VCTCGAGB	1 cut(s)	806
PstI	CTGCAG	2 cut(s)	1393, 1404
PsuI	RGATCY	1 cut(s)	976
PsyI	GACNNNGTC	1 cut(s)	1247
PvuII	CAGCTG	1 cut(s)	1709
RsaI	GTAC	8 cut(s)	329, 422, 558, 786, 1198, 1536, 1755, 2039
RsaNI	GTAC	8 cut(s)	328, 421, 557, 785, 1197, 1535, 1754, 2038
RseI	CAYNNNNRTG	7 cut(s)	213, 323, 620, 648, 798, 1275, 2046
SapI	GCTCTTC	2 cut(s)	174, 1016
SaqAI	TTAA	5 cut(s)	963, 1163, 1425, 1853, 1961
SatI	GCNGC	6 cut(s)	133, 993, 1288, 1389, 1403, 1707
Sau3AI	GATC	7 cut(s)	640, 775, 976, 1266, 1546, 1664, 1866
Sau96I	GGNCC	5 cut(s)	117, 118, 402, 1081, 1801
SchI	GAGTC	3 cut(s)	798, 1502, 2201
ScrFI	CCNGG	7 cut(s)	122, 400, 418, 706, 925, 1121, 1848
SduI	GDGCHC	6 cut(s)	121, 220, 766, 1635, 1657, 2022
SfaNI	GCATC	3 cut(s)	577, 979, 2180
SfcI	CTRYAG	2 cut(s)	1389, 1400
Sfr274I	CTCGAG	2 cut(s)	806, 1830
SinI	GGWCC	2 cut(s)	402, 1081
SlaI	CTCGAG	2 cut(s)	806, 1830
SmiMI	CAYNNNNRTG	7 cut(s)	213, 323, 620, 648, 798, 1275, 2046
SmlI	CTYRAG	6 cut(s)	765, 806, 1215, 1361, 1517, 1830
SmoI	CTYRAG	6 cut(s)	765, 806, 1215, 1361, 1517, 1830
SphI	GCATGC	1 cut(s)	681
SseBI	AGGCCT	1 cut(s)	243
SspI	AATATT	1 cut(s)	1068
SspMI	CTAG	2 cut(s)	959, 2057
StuI	AGGCCT	1 cut(s)	243
StyD4I	CCNGG	7 cut(s)	120, 398, 416, 704, 923, 1119, 1846
StyI	CCWWGG	2 cut(s)	1200, 1660
TaaI	ACNGT	6 cut(s)	818, 1001, 1249, 1692, 1959, 2216
TaiI	ACGT	2 cut(s)	760, 1425
TaqI	TCGA	5 cut(s)	369, 436, 807, 1579, 1831
TatI	WGTACW	2 cut(s)	784, 1753
TauI	GCSGC	2 cut(s)	135, 1290
TfiI	GAWTC	5 cut(s)	701, 709, 1433, 1637, 1921
Tru1I	TTAA	5 cut(s)	963, 1163, 1425, 1853, 1961
Tru9I	TTAA	5 cut(s)	963, 1163, 1425, 1853, 1961
TscAI	CASTG	4 cut(s)	185, 752, 958, 1404
TseFI	GTSAC	3 cut(s)	394, 949, 1241
TseI	GCWGC	4 cut(s)	992, 1388, 1402, 1706
Tsp45I	GTSAC	3 cut(s)	394, 949, 1241
TspDTI	ATGAA	9 cut(s)	387, 1199, 1731, 1835, 2093, 2097, 2138, 2149, 2177
TspRI	CASTG	4 cut(s)	185, 752, 958, 1404
Tth111I	GACNNNGTC	1 cut(s)	1247
VneI	GTGCAC	1 cut(s)	1653
VpaK11BI	GGWCC	2 cut(s)	402, 1081
XapI	RAATTY	3 cut(s)	335, 724, 1126
XceI	RCATGY	4 cut(s)	216, 265, 328, 681
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2231
XhoI	CTCGAG	2 cut(s)	806, 1830
XmiI	GTMKAC	1 cut(s)	1145
XspI	CTAG	2 cut(s)	959, 2057
Zsp2I	ATGCAT	1 cut(s)	1276

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.