Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001522

Physical Location & Seq

Genomic Coordinates Forward (+)

1300414 .. 1302702

Gene Structure 2289 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G000840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2289 bp

ATGCTACGGCGATGTGCTCGCTCGTGTCTCATATCTCGGAGAACCTTTAGCTCAATCAACCTTCGTGTCCTTCCCGCACCTCCCAGCCTCAACCTACAAACAAATAACCTTCTCGGCGAAGTGCGAGCCCTGGCCACACGCGGCCAACTAAACGAAGCGCTCTCACTCTTCTACACTCTTCAACCGCCACCAGCTCAAACTCACTGCAACCAAACCTACGCCACTCTCTTCCACGCATGCGCTCGCCACTACTCCCTCCAACAAGGCCTCTCTCTGCACCATTACATGCTTGCCCACAACCCCATCACTCCTCATCCCGACCTCTTTGTCTCCAACCACCTTATCAACATGTACGCCAAGTTTGGCTACTTAGACGACGCCCACCAGCTGTTCGATGAAATGCCCAGAAGAAACCTCGTCACCTGGACCGCTCTCATTTCCGGGTACGCACAGCGGAGTCGAGCTCACAATTGTTTCCGTTTATTTGCTTCCATGTTGGCGCATTACCTCCCAAATGAGTTTGCATTTGCCAGCGTGCTAAGTGCGTGTGCTGATCGCGATGGTGGATATGGCCGGCAAGTACACGCCCTCGCTTTGAAAATGTCTTTGGATGCTTGCGTTTACGCTGCGAACGCTCTCATTACCATGTACAGCAAGGTGTGTGATCATGGAGGGGTCTTTGATGTTAGTAGAGACGAGGCATGGAAGGTGTTTAGAAGCATGGAATCCCGGAATCTTATATCCTGGAATTCCATGATTGCAGGGTTTCAGTGTAGTCGACTTGGAGCTCAAGCTATTGATCTTTTTGTCCAAATGCACCTTGATGGGCTCGAGTTTGACCGTGCCACACTTCTCAGCGTTTTTTCTTCCTTGAATGGAGTAAATGGCATTGATGATATAGTGGTCACTAAGTTTTGTTATCAACTGCATTGTCTGGTTATCAAAACCGGGTTTATAGTAGGAATTGAAGTGGTCACTGCACTGGTTAAAGCTTATTCAGATCTTGGAGGGGATGTTGCTGACTGTTACAGGCTCTTCTCTGAGGCAAGCTGTCATCGAGATATTGTTGCGTGGACGGGCATTATGACAATATTTTCCCAGCGGGACCCTGAAGAAGCACTGTCCCTTTTTCGTCAATTACGCCGGGAGAATTTAGCTCCAGACAGGTATACCTTCTCCATTGTTTTAAAAACCTACGCAAGCCTTGCAACCGAGCACCATACCTCGGCAGTTCATTCTCAAGTTATCAGAGGCGGGTTTGGGGGTGACACAGTCCTTGCAAACGCCTTGATCCATGCATATGCTCGGTGCGGCTCCATTTCCTTGTCTAAGCAAGCTTTTGATGGAATTGAGTTTCGTGATGTTGTTTCTTGGAACACAATGCTCAAGGCTTATGCCTTGTATGGACAAGCTGCAGAGGCACTGCAGCTTTTTTCACAAATGGACGTTAAACCTGATTCTGCTACATTTGTTTCTCTCCTCTGTGCTTGTAGTCATGCTGGACTGGTAGAAGAAGGAACCAGAATCTTTGACTCCATGCTTGAGAGATATGGCATTGTACCTCTATGTGATCATTATGCCTGCATGGTTGACATTCTTGGTCGAGCTGGACGAGTTGGTGAGGCTGAGAAGCTTGTAAGTAGAATGCCAATGGAGCCCGATTCTGTTGTTTGGAGTGCACTCCTTGGATCATGTAGGAAGCATGGAAATACACAGTTGGCCAAGTTAGCAGCTGATAGACTGAAGGAGTTGGCTCCAGAGGGTTCATTAGTTTATGTACAAATGTCAAATATATATAGCTCTGATGGTAACTTTGGTGAAGCGGGCCTGATTAGGAAGGAAATGAAAGGGTGTCGTGTGAAAAAGGAACCTGGATTAAGCTGGATTGAGATCAGAAATCAGGTGCATGAGTTCTCCTCTGGAGGCCGACGCCATCCAGAAAGGAATCTTATAAGCAGAAAACTAAAAGAACTGATTGGACAGTTAAGGGAAATTGGTTATGTTCCAGATACAAGCTCATCCTTGCATGATGTGGAAGAGGAGCACAAAGAGGAACAATTGTACCATCATAGTGAGAAGCTAGCTTTAGTGTTTGCCATTATGAATGAAAGCAGTTTACATTGCGGTAGGACTGGTATAAAAATAATGAAGAATATCAGAGTATGCGTGGATTGCCATAACTTCATGAAGTTAGCATCAAATCTACTTCAAAAGGTTATTGTTGTGAGAGATTCAAACCGTTTCCACCACTTTAAAGATGGCATATGTTCTTGCAATGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

762

Amino Acids

85.35

Weight (kDa)

7.77

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	354 - 400	1.9e-07	PPR repeat family
PPR_2	PF13041	453 - 498	9e-09	PPR repeat family
E_motif	PF20431	568 - 630	4.3e-14	E motif
DYW_deaminase	PF14432	666 - 762	3.5e-35	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71420
fragaria_vesca	FvH4_2g00530 FvH4_2g00530 FvH4_2g00560 FvH4_2g00560 FvH4_2g00560 FvH4_2g00560
malus_domestica	MD05G1002500.v1.1
prunus_persica	Prupe.8G005700_v2.0.a1
pyrus_communis	pycom05g00240
rosa_chinensis	RchiOBHm_Chr6g0250571 RchiOBHm_Chr6g0250641 RchiOBHm_Chr6g0250661 RchiOBHm_Chr6g0250741 RchiOBHm_Chr6g0250751 RchiOBHm_Chr6g0250881 RchiOBHm_Chr6g0250901 RchiOBHm_Chr6g0250941 RchiOBHm_Chr6g0251021
rosa_laevigata	RLG00000001886 RLG00000015490 RLG00000015491 RLG00000015495 RLG00000015496 RLG00000015497 RLG00000015516 RLG00000015517 RLG00000015521 RLG00000015534
rosa_multiflora	Rmu_co8213794.1_g000001 Rmu_co8225142.1_g000001 Rmu_co8465043.1_g000001 Rmu_sc0001209.1_g000010 Rmu_sc0001209.1_g000028 Rmu_sc0001209.1_g000030 Rmu_sc0003527.1_g000008 Rmu_sc0026821.1_g000002 Rmu_sc0031313.1_g000001 Rmu_sc0037610.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217150
rosa_rugosa	Rorug05G0498000 Rorug05G0499000 Rorug05G0499100 Rorug05G0499400
rosa_samantha	Rh6AG006800 Rh6AG007100 Rh6BG003500 Rh6BG003800 Rh6DG005100
rosa_wichuraiana	Rw6G000790 Rw6G000840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1952
AasI	GACNNNNNNGTC	1 cut(s)	326
AccBSI	CCGCTC	1 cut(s)	431
AccI	GTMKAC	2 cut(s)	778, 1169
AccII	CGCG	2 cut(s)	141, 558
AclWI	GGATC	2 cut(s)	1285, 1696
AcoI	YGGCCR	4 cut(s)	132, 142, 571, 1719
AcsI	RAATTY	2 cut(s)	748, 1150
AcuI	CTGAAG	2 cut(s)	1131, 1763
AcyI	GRCGYC	2 cut(s)	378, 1930
AfaI	GTAC	7 cut(s)	353, 446, 582, 650, 1560, 1779, 2063
AfeI	AGCGCT	1 cut(s)	159
AfiI	CCNNNNNNNGG	1 cut(s)	1225
AflIII	ACRYGT	1 cut(s)	348
AgsI	TTSAA	6 cut(s)	182, 598, 874, 968, 2210, 2235
AjnI	CCWGG	4 cut(s)	129, 422, 743, 1870
AjuI	GAANNNNNNNTTGG	2 cut(s)	590, 622
Alw21I	GWGCWC	6 cut(s)	19, 466, 790, 1218, 1681, 2046
Alw26I	GTCTC	3 cut(s)	32, 334, 687
Alw44I	GTGCAC	1 cut(s)	1677
AlwI	GGATC	2 cut(s)	1285, 1696
Ama87I	CYCGRG	1 cut(s)	830
Aor51HI	AGCGCT	1 cut(s)	159
AoxI	GGCC	7 cut(s)	132, 142, 265, 571, 1719, 1825, 1924
ApaLI	GTGCAC	1 cut(s)	1677
ApeKI	GCWGC	4 cut(s)	626, 1412, 1426, 1730
ApoI	RAATTY	2 cut(s)	748, 1150
AspLEI	GCGC	3 cut(s)	160, 242, 502
AspS9I	GGNCC	3 cut(s)	426, 1105, 1825
AsuC2I	CCSGG	4 cut(s)	442, 730, 949, 1145
AsuHPI	GGTGA	4 cut(s)	412, 1277, 1631, 1829
AsuNHI	GCTAGC	1 cut(s)	2080
AvaI	CYCGRG	1 cut(s)	830
AvaII	GGWCC	2 cut(s)	426, 1105
BaeGI	GKGCMC	1 cut(s)	1681
BaeI	ACNNNNGTAYC	2 cut(s)	2045, 2078
BalI	TGGCCA	2 cut(s)	134, 1721
BanII	GRGCYC	5 cut(s)	130, 466, 790, 831, 1659
BauI	CACGAG	1 cut(s)	22
Bbv12I	GWGCWC	6 cut(s)	19, 466, 790, 1218, 1681, 2046
BbvI	GCAGC	4 cut(s)	613, 1399, 1438, 1742
BccI	CCATC	8 cut(s)	311, 554, 818, 1337, 1799, 1941, 2073, 2252
BceAI	ACGGC	1 cut(s)	23
BciT130I	CCWGG	4 cut(s)	131, 424, 745, 1872
BclI	TGATCA	2 cut(s)	664, 1570
BcnI	CCSGG	4 cut(s)	442, 730, 949, 1145
BcoDI	GTCTC	3 cut(s)	32, 334, 687
BfaI	CTAG	1 cut(s)	2081
BfmI	CTRYAG	2 cut(s)	1413, 1424
BfoI	RGCGCY	1 cut(s)	161
BglII	AGATCT	1 cut(s)	1000
BisI	GCNGC	6 cut(s)	142, 627, 1312, 1413, 1427, 1731
BlsI	GCNGC	6 cut(s)	143, 628, 1313, 1414, 1428, 1732
Bme1390I	CCNGG	8 cut(s)	131, 424, 442, 730, 745, 949, 1145, 1872
Bme18I	GGWCC	2 cut(s)	426, 1105
BmeT110I	CYCGRG	1 cut(s)	830
BmgT120I	GGNCC	3 cut(s)	426, 1105, 1825
BmiI	GGNNCC	7 cut(s)	1106, 1107, 1315, 1519, 1656, 1755, 1869
BmrFI	CCNGG	8 cut(s)	131, 424, 442, 730, 745, 949, 1145, 1872
BmsI	GCATC	2 cut(s)	601, 2204
BmtI	GCTAGC	1 cut(s)	2084
BplI	GAGNNNNNCTC	4 cut(s)	448, 480, 1901, 1933
BpmI	CTGGAG	3 cut(s)	1143, 1740, 1941
BpuEI	CTTGAG	4 cut(s)	774, 1224, 1370, 1562
BpuMI	CCSGG	4 cut(s)	442, 730, 949, 1145
BsaBI	GATNNNNATC	1 cut(s)	1889
BsaHI	GRCGYC	2 cut(s)	378, 1930
BsaJI	CCNNGG	3 cut(s)	129, 1224, 1684
BsaXI	ACNNNNNCTCC	2 cut(s)	999, 1029
Bsc4I	CCNNNNNNNGG	1 cut(s)	1225
Bse118I	RCCGGY	1 cut(s)	573
Bse1I	ACTGG	4 cut(s)	987, 1509, 2137, 2288
Bse3DI	GCAATG	2 cut(s)	2119, 2281
Bse8I	GATNNNNATC	1 cut(s)	1889
BseBI	CCWGG	4 cut(s)	131, 424, 745, 1872
BseDI	CCNNGG	3 cut(s)	129, 1224, 1684
BseGI	GGATG	5 cut(s)	313, 616, 1018, 1933, 2018
BseJI	GATNNNNATC	1 cut(s)	1889
BseLI	CCNNNNNNNGG	1 cut(s)	1225
BseMI	GCAATG	2 cut(s)	2119, 2281
BseMII	CTCAG	3 cut(s)	868, 1032, 1617
BseNI	ACTGG	4 cut(s)	987, 1509, 2137, 2288
BseRI	GAGGAG	4 cut(s)	300, 1469, 1906, 2054
BseSI	GKGCMC	1 cut(s)	1681
BseXI	GCAGC	4 cut(s)	613, 1399, 1438, 1742
BseYI	CCCAGC	2 cut(s)	83, 1098
BsgI	GTGCAG	2 cut(s)	260, 963
Bsh1236I	CGCG	2 cut(s)	141, 558
BshFI	GGCC	7 cut(s)	134, 144, 267, 573, 1721, 1827, 1926
BsiHKAI	GWGCWC	6 cut(s)	19, 466, 790, 1218, 1681, 2046
BsiHKCI	CYCGRG	1 cut(s)	830
BsiSI	CCGG	5 cut(s)	441, 574, 730, 948, 1144
BslFI	GGGAC	2 cut(s)	1108, 1118
BslI	CCNNNNNNNGG	1 cut(s)	1225
BsmAI	GTCTC	3 cut(s)	32, 334, 687
BsmBI	CGTCTC	1 cut(s)	687
BsmFI	GGGAC	2 cut(s)	1108, 1118
BsmI	GAATGC	1 cut(s)	1650
BsnI	GGCC	7 cut(s)	134, 144, 267, 573, 1721, 1827, 1926
BsoBI	CYCGRG	1 cut(s)	830
Bsp1286I	GDGCHC	9 cut(s)	19, 130, 466, 790, 831, 1218, 1659, 1681, 2046
Bsp1407I	TGTACA	2 cut(s)	648, 1777
Bsp143I	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
Bsp68I	TCGCGA	1 cut(s)	558
BspANI	GGCC	7 cut(s)	134, 144, 267, 573, 1721, 1827, 1926
BspCNI	CTCAG	3 cut(s)	867, 1033, 1618
BspFNI	CGCG	2 cut(s)	141, 558
BspHI	TCATGA	1 cut(s)	2184
BspLI	GGNNCC	7 cut(s)	1106, 1107, 1315, 1519, 1656, 1755, 1869
BspMAI	CTGCAG	2 cut(s)	1417, 1428
BspOI	GCTAGC	1 cut(s)	2084
BspPI	GGATC	2 cut(s)	1285, 1696
BspQI	GCTCTTC	1 cut(s)	1040
BsrBI	CCGCTC	1 cut(s)	431
BsrDI	GCAATG	2 cut(s)	2119, 2281
BsrFI	RCCGGY	1 cut(s)	573
BsrGI	TGTACA	2 cut(s)	648, 1777
BsrI	ACTGG	4 cut(s)	987, 1509, 2137, 2288
BssAI	RCCGGY	1 cut(s)	573
BssECI	CCNNGG	3 cut(s)	129, 1224, 1684
BssMI	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
BssNAI	GTATAC	1 cut(s)	1170
BssNI	GRCGYC	2 cut(s)	378, 1930
BssSI	CACGAG	1 cut(s)	22
BssT1I	CCWWGG	1 cut(s)	1684
Bst1107I	GTATAC	1 cut(s)	1170
Bst2BI	CACGAG	1 cut(s)	22
Bst2UI	CCWGG	4 cut(s)	131, 424, 745, 1872
Bst4CI	ACNGT	7 cut(s)	842, 1025, 1122, 1273, 1716, 1983, 2240
Bst6I	CTCTTC	5 cut(s)	173, 183, 233, 1040, 2031
BstACI	GRCGYC	2 cut(s)	378, 1930
BstAPI	GCANNNNNTGC	1 cut(s)	1205
BstAUI	TGTACA	2 cut(s)	648, 1777
BstDEI	CTNAG	7 cut(s)	370, 539, 854, 909, 1041, 1329, 1626
BstF5I	GGATG	5 cut(s)	313, 616, 1018, 1933, 2018
BstFNI	CGCG	2 cut(s)	141, 558
BstH2I	RGCGCY	1 cut(s)	161
BstHHI	GCGC	3 cut(s)	160, 242, 502
BstKTI	GATC	8 cut(s)	556, 667, 802, 1003, 1293, 1573, 1691, 1893
BstMAI	GTCTC	3 cut(s)	32, 334, 687
BstMBI	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
BstMWI	GCNNNNNNNGC	6 cut(s)	632, 1205, 1418, 1654, 1727, 2172
BstNI	CCWGG	4 cut(s)	131, 424, 745, 1872
BstNSI	RCATGY	3 cut(s)	240, 289, 352
BstSCI	CCNGG	8 cut(s)	129, 422, 440, 728, 743, 947, 1143, 1870
BstSFI	CTRYAG	2 cut(s)	1413, 1424
BstSLI	GKGCMC	1 cut(s)	1681
BstUI	CGCG	2 cut(s)	141, 558
BstV1I	GCAGC	4 cut(s)	613, 1399, 1438, 1742
BstX2I	RGATCY	1 cut(s)	1000
BstYI	RGATCY	1 cut(s)	1000
BstZ17I	GTATAC	1 cut(s)	1170
BsuRI	GGCC	7 cut(s)	134, 144, 267, 573, 1721, 1827, 1926
BtgZI	GCGATG	2 cut(s)	25, 573
BtsCI	GGATG	5 cut(s)	313, 616, 1018, 1933, 2018
BtsI	GCAGTG	3 cut(s)	202, 975, 1421
BtsIMutI	CAGTG	6 cut(s)	202, 776, 975, 980, 1118, 1421
BtuMI	TCGCGA	1 cut(s)	558
CciI	TCATGA	1 cut(s)	2184
CfoI	GCGC	3 cut(s)	160, 242, 502
Cfr10I	RCCGGY	1 cut(s)	573
Cfr13I	GGNCC	3 cut(s)	426, 1105, 1825
CseI	GACGC	2 cut(s)	386, 1938
Csp6I	GTAC	7 cut(s)	352, 445, 581, 649, 1559, 1778, 2062
CviQI	GTAC	7 cut(s)	352, 445, 581, 649, 1559, 1778, 2062
DdeI	CTNAG	7 cut(s)	370, 539, 854, 909, 1041, 1329, 1626
DpnI	GATC	8 cut(s)	555, 666, 801, 1002, 1292, 1572, 1690, 1892
DpnII	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
DraI	TTTAAA	2 cut(s)	1188, 2254
DrdI	GACNNNNNNGTC	1 cut(s)	326
DseDI	GACNNNNNNGTC	1 cut(s)	326
EaeI	YGGCCR	4 cut(s)	132, 142, 571, 1719
Eam1104I	CTCTTC	5 cut(s)	173, 183, 233, 1040, 2031
EarI	CTCTTC	5 cut(s)	173, 183, 233, 1040, 2031
Ecl136II	GAGCTC	2 cut(s)	464, 788
Eco130I	CCWWGG	1 cut(s)	1684
Eco147I	AGGCCT	1 cut(s)	267
Eco24I	GRGCYC	5 cut(s)	130, 466, 790, 831, 1659
Eco47I	GGWCC	2 cut(s)	426, 1105
Eco47III	AGCGCT	1 cut(s)	159
Eco53kI	GAGCTC	2 cut(s)	464, 788
Eco57I	CTGAAG	2 cut(s)	1131, 1763
Eco88I	CYCGRG	1 cut(s)	830
EcoICRI	GAGCTC	2 cut(s)	464, 788
EcoO109I	RGGNCCY	1 cut(s)	1105
EcoRI	GAATTC	1 cut(s)	748
EcoRII	CCWGG	4 cut(s)	129, 422, 743, 1870
EcoT14I	CCWWGG	1 cut(s)	1684
EcoT22I	ATGCAT	1 cut(s)	1300
EcoT38I	GRGCYC	5 cut(s)	130, 466, 790, 831, 1659
ErhI	CCWWGG	1 cut(s)	1684
Esp3I	CGTCTC	1 cut(s)	687
FaqI	GGGAC	2 cut(s)	1108, 1118
FauI	CCCGC	4 cut(s)	82, 1095, 1247, 1816
FauNDI	CATATG	2 cut(s)	1300, 2264
FbaI	TGATCA	2 cut(s)	664, 1570
FblI	GTMKAC	2 cut(s)	778, 1169
Fnu4HI	GCNGC	6 cut(s)	142, 627, 1312, 1413, 1427, 1731
FokI	GGATG	5 cut(s)	300, 623, 1025, 1920, 2005
FriOI	GRGCYC	5 cut(s)	130, 466, 790, 831, 1659
Fsp4HI	GCNGC	6 cut(s)	142, 627, 1312, 1413, 1427, 1731
FspBI	CTAG	1 cut(s)	2081
GlaI	GCGC	3 cut(s)	159, 241, 501
GluI	GCNGC	6 cut(s)	142, 627, 1312, 1413, 1427, 1731
GsaI	CCCAGC	2 cut(s)	87, 1102
GsuI	CTGGAG	3 cut(s)	1143, 1740, 1941
HaeII	RGCGCY	1 cut(s)	161
HaeIII	GGCC	7 cut(s)	134, 144, 267, 573, 1721, 1827, 1926
HapII	CCGG	5 cut(s)	441, 574, 730, 948, 1144
HgaI	GACGC	2 cut(s)	386, 1938
HhaI	GCGC	3 cut(s)	160, 242, 502
Hin1I	GRCGYC	2 cut(s)	378, 1930
Hin6I	GCGC	3 cut(s)	158, 240, 500
HinP1I	GCGC	3 cut(s)	158, 240, 500
HincII	GTYRAC	2 cut(s)	779, 1591
HindII	GTYRAC	2 cut(s)	779, 1591
HindIII	AAGCTT	3 cut(s)	990, 1335, 1631
HinfI	GANTC	9 cut(s)	457, 725, 733, 1457, 1524, 1532, 1661, 1945, 2231
HpaII	CCGG	5 cut(s)	441, 574, 730, 948, 1144
HphI	GGTGA	4 cut(s)	412, 1277, 1631, 1829
Hpy166II	GTNNAC	8 cut(s)	583, 622, 779, 1074, 1170, 1591, 1679, 2117
Hpy188I	TCNGA	7 cut(s)	39, 1000, 1042, 1250, 1804, 1895, 2159
Hpy8I	GTNNAC	8 cut(s)	583, 622, 779, 1074, 1170, 1591, 1679, 2117
Hpy99I	CGWCG	2 cut(s)	380, 1932
HpyAV	CCTTC	8 cut(s)	71, 80, 119, 700, 1183, 1508, 1738, 1831
HpyCH4III	ACNGT	7 cut(s)	842, 1025, 1122, 1273, 1716, 1983, 2240
HpyCH4IV	ACGT	1 cut(s)	1446
HpyF10VI	GCNNNNNNNGC	6 cut(s)	632, 1205, 1418, 1654, 1727, 2172
HpyF3I	CTNAG	7 cut(s)	370, 539, 854, 909, 1041, 1329, 1626
HpySE526I	ACGT	1 cut(s)	1446
Hsp92I	GRCGYC	2 cut(s)	378, 1930
HspAI	GCGC	3 cut(s)	158, 240, 500
KflI	GGGWCCC	1 cut(s)	1105
KroI	GCCGGC	1 cut(s)	573
KroNI	GCCGGC	1 cut(s)	575
Ksp22I	TGATCA	2 cut(s)	664, 1570
Kzo9I	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
LguI	GCTCTTC	1 cut(s)	1040
LmnI	GCTCC	6 cut(s)	785, 1162, 1319, 1654, 1759, 2041
Lsp1109I	GCAGC	4 cut(s)	613, 1399, 1438, 1742
LweI	GCATC	2 cut(s)	601, 2204
MaeI	CTAG	1 cut(s)	2081
MaeII	ACGT	1 cut(s)	1446
MaeIII	GTNAC	6 cut(s)	418, 904, 973, 1025, 1265, 1808
MalI	GATC	8 cut(s)	555, 666, 801, 1002, 1292, 1572, 1690, 1892
MbiI	CCGCTC	1 cut(s)	431
MboI	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
MfeI	CAATTG	2 cut(s)	469, 2057
MflI	RGATCY	1 cut(s)	1000
MhlI	GDGCHC	9 cut(s)	19, 130, 466, 790, 831, 1218, 1659, 1681, 2046
MlsI	TGGCCA	2 cut(s)	134, 1721
MluCI	AATT	8 cut(s)	469, 748, 963, 1136, 1150, 1347, 1992, 2057
MluNI	TGGCCA	2 cut(s)	134, 1721
MlyI	GAGTC	2 cut(s)	466, 1526
MmeI	TCCRAC	2 cut(s)	283, 357
Mox20I	TGGCCA	2 cut(s)	134, 1721
Mph1103I	ATGCAT	1 cut(s)	1300
MroNI	GCCGGC	1 cut(s)	573
MscI	TGGCCA	2 cut(s)	134, 1721
MseI	TTAA	6 cut(s)	987, 1187, 1449, 1877, 1985, 2253
MslI	CAYNNNNRTG	4 cut(s)	644, 822, 1299, 2070
Msp20I	TGGCCA	2 cut(s)	134, 1721
MspA1I	CMGCKG	4 cut(s)	388, 454, 1102, 1733
MspI	CCGG	5 cut(s)	441, 574, 730, 948, 1144
MspR9I	CCNGG	8 cut(s)	131, 424, 442, 730, 745, 949, 1145, 1872
MunI	CAATTG	2 cut(s)	469, 2057
Mva1269I	GAATGC	1 cut(s)	1650
MvaI	CCWGG	4 cut(s)	131, 424, 745, 1872
MvnI	CGCG	2 cut(s)	141, 558
MwoI	GCNNNNNNNGC	6 cut(s)	632, 1205, 1418, 1654, 1727, 2172
NaeI	GCCGGC	1 cut(s)	575
NciI	CCSGG	4 cut(s)	442, 730, 949, 1145
NdeI	CATATG	2 cut(s)	1300, 2264
NdeII	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
NgoMIV	GCCGGC	1 cut(s)	573
NheI	GCTAGC	1 cut(s)	2080
NlaIV	GGNNCC	7 cut(s)	1106, 1107, 1315, 1519, 1656, 1755, 1869
NmeAIII	GCCGAG	2 cut(s)	93, 1205
NmuCI	GTSAC	4 cut(s)	418, 904, 973, 1265
NruI	TCGCGA	1 cut(s)	558
NsiI	ATGCAT	1 cut(s)	1300
NspI	RCATGY	3 cut(s)	240, 289, 352
PaeI	GCATGC	1 cut(s)	240
PaeR7I	CTCGAG	1 cut(s)	830
PagI	TCATGA	1 cut(s)	2184
PceI	AGGCCT	1 cut(s)	267
PciI	ACATGT	1 cut(s)	348
PciSI	GCTCTTC	1 cut(s)	1040
PcsI	WCGNNNNNNNCGW	1 cut(s)	1609
PctI	GAATGC	1 cut(s)	1650
PdiI	GCCGGC	1 cut(s)	575
PfeI	GAWTC	7 cut(s)	725, 733, 1457, 1524, 1661, 1945, 2231
PflFI	GACNNNGTC	1 cut(s)	1271
PfoI	TCCNGGA	2 cut(s)	728, 743
PkrI	GCNGC	6 cut(s)	143, 628, 1313, 1414, 1428, 1732
PleI	GAGTC	2 cut(s)	465, 1526
PpsI	GAGTC	2 cut(s)	465, 1526
PpuMI	RGGWCCY	1 cut(s)	1105
PscI	ACATGT	1 cut(s)	348
PsiI	TTATAA	1 cut(s)	1952
Psp124BI	GAGCTC	2 cut(s)	466, 790
Psp5II	RGGWCCY	1 cut(s)	1105
Psp6I	CCWGG	4 cut(s)	129, 422, 743, 1870
PspFI	CCCAGC	2 cut(s)	83, 1098
PspGI	CCWGG	4 cut(s)	129, 422, 743, 1870
PspN4I	GGNNCC	7 cut(s)	1106, 1107, 1315, 1519, 1656, 1755, 1869
PspPI	GGNCC	3 cut(s)	426, 1105, 1825
PspPPI	RGGWCCY	1 cut(s)	1105
PspXI	VCTCGAGB	1 cut(s)	830
PstI	CTGCAG	2 cut(s)	1417, 1428
PsuI	RGATCY	1 cut(s)	1000
PsyI	GACNNNGTC	1 cut(s)	1271
PvuII	CAGCTG	2 cut(s)	388, 1733
RruI	TCGCGA	1 cut(s)	558
RsaI	GTAC	7 cut(s)	353, 446, 582, 650, 1560, 1779, 2063
RsaNI	GTAC	7 cut(s)	352, 445, 581, 649, 1559, 1778, 2062
RseI	CAYNNNNRTG	4 cut(s)	644, 822, 1299, 2070
SacI	GAGCTC	2 cut(s)	466, 790
SalI	GTCGAC	1 cut(s)	777
SapI	GCTCTTC	1 cut(s)	1040
SaqAI	TTAA	6 cut(s)	987, 1187, 1449, 1877, 1985, 2253
SatI	GCNGC	6 cut(s)	142, 627, 1312, 1413, 1427, 1731
Sau3AI	GATC	8 cut(s)	553, 664, 799, 1000, 1290, 1570, 1688, 1890
Sau96I	GGNCC	3 cut(s)	426, 1105, 1825
SchI	GAGTC	2 cut(s)	466, 1526
ScrFI	CCNGG	8 cut(s)	131, 424, 442, 730, 745, 949, 1145, 1872
SduI	GDGCHC	9 cut(s)	19, 130, 466, 790, 831, 1218, 1659, 1681, 2046
SfaNI	GCATC	2 cut(s)	601, 2204
SfcI	CTRYAG	2 cut(s)	1413, 1424
Sfr274I	CTCGAG	1 cut(s)	830
SinI	GGWCC	2 cut(s)	426, 1105
SlaI	CTCGAG	1 cut(s)	830
SmiMI	CAYNNNNRTG	4 cut(s)	644, 822, 1299, 2070
SmlI	CTYRAG	5 cut(s)	789, 830, 1239, 1385, 1541
SmoI	CTYRAG	5 cut(s)	789, 830, 1239, 1385, 1541
SphI	GCATGC	1 cut(s)	240
Sse9I	AATT	8 cut(s)	469, 748, 963, 1136, 1150, 1347, 1992, 2057
SseBI	AGGCCT	1 cut(s)	267
SspI	AATATT	1 cut(s)	1092
SspMI	CTAG	1 cut(s)	2081
SstI	GAGCTC	2 cut(s)	466, 790
StuI	AGGCCT	1 cut(s)	267
StyD4I	CCNGG	8 cut(s)	129, 422, 440, 728, 743, 947, 1143, 1870
StyI	CCWWGG	1 cut(s)	1684
TaaI	ACNGT	7 cut(s)	842, 1025, 1122, 1273, 1716, 1983, 2240
TaiI	ACGT	1 cut(s)	1449
TaqI	TCGA	6 cut(s)	393, 460, 778, 831, 1057, 1603
TasI	AATT	8 cut(s)	469, 748, 963, 1136, 1150, 1347, 1992, 2057
TatI	WGTACW	3 cut(s)	580, 648, 1777
TauI	GCSGC	2 cut(s)	144, 1314
TfiI	GAWTC	7 cut(s)	725, 733, 1457, 1524, 1661, 1945, 2231
Tru1I	TTAA	6 cut(s)	987, 1187, 1449, 1877, 1985, 2253
Tru9I	TTAA	6 cut(s)	987, 1187, 1449, 1877, 1985, 2253
TscAI	CASTG	6 cut(s)	209, 776, 982, 987, 1125, 1428
TseFI	GTSAC	4 cut(s)	418, 904, 973, 1265
TseI	GCWGC	4 cut(s)	626, 1412, 1426, 1730
Tsp45I	GTSAC	4 cut(s)	418, 904, 973, 1265
TspDTI	ATGAA	9 cut(s)	411, 1223, 1755, 1859, 2117, 2121, 2162, 2173, 2201
TspGWI	ACGGA	1 cut(s)	467
TspRI	CASTG	6 cut(s)	209, 776, 982, 987, 1125, 1428
Tth111I	GACNNNGTC	1 cut(s)	1271
VneI	GTGCAC	1 cut(s)	1677
VpaK11BI	GGWCC	2 cut(s)	426, 1105
XapI	RAATTY	2 cut(s)	748, 1150
XceI	RCATGY	3 cut(s)	240, 289, 352
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2255
XhoI	CTCGAG	1 cut(s)	830
XmiI	GTMKAC	2 cut(s)	778, 1169
XspI	CTAG	1 cut(s)	2081
Zsp2I	ATGCAT	1 cut(s)	1300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.