pycom07g24140

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000488
Physical Location & Seq
Reverse (-)
24911465 .. 24914962
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24140.1

Sequence Viewer

Length: 1305 bp
ATGGATGTAGACGGAGGGGGAAGCAAACGGGTCTTTTACAGACTGGGTGGGCCACAATCCGACCCATCAAAGAACCAGAAGGTATGCTACCATTGGAGAGCGGGCAAGTGCAATCGCCACCCTTGCCCTTTCCTCCACAGAGAGCTACCGGCGCCGCCGTCGCAGCAAGGTCTCAACGGGATGGCCTCGACTAAGCGCCATCACGCCTTCGCCGCTGACACCGACGGTCCATCGTCACGGAATCGGGGTCCCAATAACTTCAATGGCGGGACTTCTTCGACGTGGGGTCGTACAGGAGGAGCAAATAGGGTGTTTGTTAGGAAGATGGAGAAAGTCTGTAATTTTTGGGTTCAGGGGAATTGCAGCTATGGGGATAAGTGTAAGTTCTTGCATTCTTGGAGTATGGGGGATAGCTTCAGCTTGTTGACGCAGCTTGAGGGGCATGAGAAGGTTGTTAGTGGGATTGCACTGCCTTCTGGGTCTGATAAACTTTATACTGGGAGTAAGGATCAAACTGTGAGGGTTTGGGATTGCGCGTCTGGTCAGTGCCTGGGAGTTATTAATCTTGGCGGTGAAGTAGGGTGTATGATCAGTGAAGGTCCTTGGATTTTTGTTGGCATACCAGAAGCTGTAAAGGCATGGAACACCCAGACTAACTCTGAACTGAGTCTTAGTGGGCCTGTCGGGCAAGTTTATGCCATGGTTGTGGGAAATGATTTGCTCTTTGCTGGTACACAGGATGGTTCTATATTGGCATGGAAGTTTAATGCTGTTACTAATTGCTTCGAACCAGCTGCATCACTAATAGGTCACACTCTTGCAGTTGTTTCATTAGTAGTTGGAGCAAATAGGCTCTACTCTGGTTCAATGGATCCTTCTATAAGGGTCTGGAGCCTGGAAACTTTGCAGTGTATACAAACGCTAACAGAGCATACTGAAGTTGTCATGTCTGTTCTTTGCTGGGATCAGTTTCTTTTATCATGTTCCTTGGATCGACAATTAAAGGTCTGGGTTGCTACTCAAAGTGGAAACTTGGAAGCAACATATACTCACACGGAAGAACATGGTTTGATAACACTCTGTGGGATGCATGATTCAGAAGCCAAGCCAGTTTTACTGTGCGCTTGCAACGACAACACTGTCCGCATATATGATTTACCATCGTTTTCGGAGAGGGGTAAAATATTTTCAAAACAGGAGATAAGATCGATTCAGGTAGGCCCTGGTGGCTTGTTTTTCACGGGTGATGGAACCGGTCAAGTGAAAGTATGGAAATGGACTGAACCAGCTGCGGTCACTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
WD40 zf-CCCH

Protein Analysis

435

Amino Acids

47.18

Weight (kDa)

7.14

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_4 PF18044 111 - 131 1.1e-07 CCCH-type zinc finger
zf-CCCH PF00642 112 - 132 8.1e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf_CCCH_4 PF18345 113 - 131 1.1e-07 Zinc finger domain
Beta-prop_WDR5 PF25175 131 - 186 1.5e-07 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 133 - 318 2.2e-21 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 141 - 184 2.7e-06 WDR3 first beta-propeller domain
WD40 PF00400 141 - 177 5.1e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 154 - 342 1.1e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 200 - 350 3.4e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 203 - 386 2.2e-19 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 206 - 428 4e-12 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 211 - 388 1.1e-15 WDR5 beta-propeller domain
Beta-prop_IP5PC_F PF23754 233 - 315 9.8e-06 IP5P C-F beta-propeller
WDR55 PF24796 238 - 386 1.4e-06 WDR55
Beta-prop_TEP1_2nd PF25047 240 - 353 2.3e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 262 - 385 1.7e-09 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 280 - 388 4.6e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 283 - 424 6.3e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 283 - 430 4.2e-14 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 283 - 428 2.9e-14 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25440 AT4G25440 AT4G25440 AT5G51980 AT5G51980
fragaria_vesca FvH4_2g11580 FvH4_3g29280 FvH4_4g05980 FvH4_7g28880 FvH4_7g28880
malus_domestica MD01G1195600.v1.1 MD02G1195200.v1.1 MD07G1262400.v1.1
prunus_persica Prupe.2G102200_v2.0.a1 Prupe.2G102200_v2.0.a1 Prupe.2G120800_v2.0.a1 Prupe.2G120800_v2.0.a1 Prupe.2G153300_v2.0.a1 Prupe.2G153300_v2.0.a1 Prupe.2G153400_v2.0.a1 Prupe.2G153400_v2.0.a1 Prupe.2G288900_v2.0.a1
pyrus_communis pycom01g20620 pycom02g15900 pycom07g24140
rosa_chinensis RchiOBHm_Chr1g0376561 RchiOBHm_Chr3g0483051 RchiOBHm_Chr3g0483061 RchiOBHm_Chr3g0483601 RchiOBHm_Chr3g0483611 RchiOBHm_Chr4g0402321 RchiOBHm_Chr6g0270771
rosa_laevigata RLG00000009464 RLG00000009486 RLG00000013770 RLG00000023264 RLG00000023325 RLG00000026601 RLG00000035049
rosa_multiflora Rmu_co8110388.1_g000001 Rmu_co8419409.1_g000001 Rmu_sc0000555.1_g000012 Rmu_sc0000642.1_g000020 Rmu_sc0001308.1_g000010 Rmu_sc0002963.1_g000021 Rmu_sc0004788.1_g000012 Rmu_sc0005594.1_g000015 Rmu_sc0022770.1_g000001
rosa_roxburghii Rroxscaffold_4G00282000 Rroxscaffold_5G00341940 Rroxscaffold_5G00341950 Rroxscaffold_6G00398950 Rroxscaffold_7G00197610
rosa_rugosa Rorug01G0396500 Rorug03G0207400 Rorug03G0346000 Rorug04G0166800 Rorug06G0056400 Rorug06G0056500
rosa_samantha Rh1AG412700 Rh1BG372400 Rh1BG372600 Rh1CG386200 Rh1CG386500 Rh1DG403300 Rh1DG403500 Rh3AG255500 Rh3AG255600 Rh3BG292600 Rh3CG290200 Rh3DG278800 Rh3DG286100 Rh4AG075500 Rh4AG111600 Rh4BG071900 Rh4BG072100 Rh4BG231300 Rh4CG080200 Rh4CG080300 Rh4DG069300 Rh4DG069500 Rh6AG175700 Rh6BG178900 Rh6CG175200 Rh6DG167500
rosa_wichuraiana Rw1G036190 Rw3G023110 Rw4G006070 Rw6G014960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1139
AccB1I GGYRCC 1 cut(s) 151
AccBSI CCGCTC 1 cut(s) 101
AccI GTMKAC 2 cut(s) 9, 913
AccII CGCG 1 cut(s) 536
AciI CCGC 7 cut(s) 101, 155, 213, 267, 570, 1144, 1292
AclWI GGATC 5 cut(s) 516, 866, 879, 972, 999
AcuI CTGAAG 2 cut(s) 400, 957
AcyI GRCGYC 1 cut(s) 152
AdeI CACNNNGTG 1 cut(s) 1082
AfaI GTAC 2 cut(s) 292, 733
AgeI ACCGGT 1 cut(s) 1253
AgsI TTSAA 3 cut(s) 262, 867, 1191
AhdI GACNNNNNGTC 1 cut(s) 285
AjiI CACGTC 1 cut(s) 282
AjnI CCWGG 3 cut(s) 549, 894, 1222
AluBI AGCT 8 cut(s) 145, 366, 414, 420, 433, 629, 794, 1289
AluI AGCT 8 cut(s) 145, 366, 414, 420, 433, 629, 794, 1289
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 5 cut(s) 516, 866, 879, 972, 999
AlwNI CAGNNNCTG 2 cut(s) 550, 629
AoxI GGCC 4 cut(s) 50, 183, 677, 1219
ApeKI GCWGC 5 cut(s) 163, 363, 430, 794, 1289
AseI ATTAAT 1 cut(s) 561
AsiGI ACCGGT 1 cut(s) 1253
AspLEI GCGC 4 cut(s) 154, 198, 536, 1124
AspS9I GGNCC 6 cut(s) 50, 227, 248, 599, 677, 1220
AsuHPI GGTGA 2 cut(s) 584, 1256
AsuII TTCGAA 1 cut(s) 786
AvaII GGWCC 3 cut(s) 227, 248, 599
BamHI GGATCC 1 cut(s) 871
BanI GGYRCC 1 cut(s) 151
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbvI GCAGC 5 cut(s) 175, 375, 442, 781, 1276
BccI CCATC 8 cut(s) 73, 175, 207, 238, 319, 734, 1168, 1241
BceAI ACGGC 1 cut(s) 142
BcgI CGANNNNNNTGC 2 cut(s) 776, 810
BciT130I CCWGG 3 cut(s) 551, 896, 1224
BclI TGATCA 1 cut(s) 588
BcoDI GTCTC 1 cut(s) 176
BfoI RGCGCY 2 cut(s) 155, 199
BglI GCCNNNNNGGC 2 cut(s) 685, 1227
BisI GCNGC 7 cut(s) 155, 164, 213, 364, 431, 795, 1290
BlsI GCNGC 7 cut(s) 156, 165, 214, 365, 432, 796, 1291
Bme1390I CCNGG 3 cut(s) 551, 896, 1224
Bme18I GGWCC 3 cut(s) 227, 248, 599
BmeRI GACNNNNNGTC 1 cut(s) 285
BmgBI CACGTC 1 cut(s) 282
BmgT120I GGNCC 6 cut(s) 50, 227, 248, 599, 677, 1220
BmiI GGNNCC 6 cut(s) 153, 249, 250, 873, 893, 1252
BmrFI CCNGG 3 cut(s) 551, 896, 1224
BmrI ACTGGG 2 cut(s) 53, 507
BmsI GCATC 2 cut(s) 806, 1077
BmuI ACTGGG 2 cut(s) 53, 507
BpmI CTGGAG 1 cut(s) 910
Bpu14I TTCGAA 1 cut(s) 786
BpuEI CTTGAG 1 cut(s) 455
Bsa29I ATCGAT 1 cut(s) 1208
BsaHI GRCGYC 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 5 cut(s) 550, 602, 699, 987, 1222
BsaWI WCCGGW 1 cut(s) 1253
Bse118I RCCGGY 2 cut(s) 148, 1253
Bse1I ACTGG 3 cut(s) 48, 502, 1109
BseBI CCWGG 3 cut(s) 551, 896, 1224
BseCI ATCGAT 1 cut(s) 1208
BseDI CCNNGG 5 cut(s) 550, 602, 699, 987, 1222
BseGI GGATG 4 cut(s) 10, 186, 745, 1092
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 3 cut(s) 48, 502, 1109
BseRI GAGGAG 1 cut(s) 312
BseXI GCAGC 5 cut(s) 175, 375, 442, 781, 1276
BseYI CCCAGC 1 cut(s) 960
Bsh1236I CGCG 1 cut(s) 536
BshFI GGCC 4 cut(s) 52, 185, 679, 1221
BshNI GGYRCC 1 cut(s) 151
BshTI ACCGGT 1 cut(s) 1253
BshVI ATCGAT 1 cut(s) 1208
BsiSI CCGG 2 cut(s) 149, 1254
BslFI GGGAC 2 cut(s) 234, 283
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 2 cut(s) 234, 283
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 4 cut(s) 52, 185, 679, 1221
Bso31I GGTCTC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 786
Bsp143I GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 7 cut(s) 101, 155, 213, 267, 570, 1144, 1292
BspANI GGCC 4 cut(s) 52, 185, 679, 1221
BspCNI CTCAG 1 cut(s) 657
BspDI ATCGAT 1 cut(s) 1208
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 6 cut(s) 153, 249, 250, 873, 893, 1252
BspPI GGATC 5 cut(s) 516, 866, 879, 972, 999
BspT104I TTCGAA 1 cut(s) 786
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 176
BsrBI CCGCTC 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 148, 1253
BsrI ACTGG 3 cut(s) 48, 502, 1109
BssAI RCCGGY 2 cut(s) 148, 1253
BssECI CCNNGG 5 cut(s) 550, 602, 699, 987, 1222
BssMI GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
BssNAI GTATAC 1 cut(s) 914
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 3 cut(s) 602, 699, 987
Bst1107I GTATAC 1 cut(s) 914
Bst2UI CCWGG 3 cut(s) 551, 896, 1224
Bst4CI ACNGT 4 cut(s) 227, 517, 1119, 1141
BstACI GRCGYC 1 cut(s) 152
BstBI TTCGAA 1 cut(s) 786
BstC8I GCNNGC 2 cut(s) 103, 1126
BstDEI CTNAG 3 cut(s) 192, 665, 671
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 4 cut(s) 10, 186, 745, 1092
BstFNI CGCG 1 cut(s) 536
BstH2I RGCGCY 2 cut(s) 155, 199
BstHHI GCGC 4 cut(s) 154, 198, 536, 1124
BstKTI GATC 6 cut(s) 511, 591, 874, 967, 994, 1208
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
BstNI CCWGG 3 cut(s) 551, 896, 1224
BstSCI CCNGG 3 cut(s) 549, 894, 1222
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 5 cut(s) 175, 375, 442, 781, 1276
BstX2I RGATCY 1 cut(s) 871
BstXI CCANNNNNNTGG 1 cut(s) 706
BstYI RGATCY 1 cut(s) 871
BstZ17I GTATAC 1 cut(s) 914
Bsu15I ATCGAT 1 cut(s) 1208
BsuRI GGCC 4 cut(s) 52, 185, 679, 1221
BsuTUI ATCGAT 1 cut(s) 1208
BtgI CCRYGG 1 cut(s) 699
BtrI CACGTC 1 cut(s) 282
BtsCI GGATG 4 cut(s) 10, 186, 745, 1092
BtsI GCAGTG 3 cut(s) 467, 914, 1296
BtsIMutI CAGTG 6 cut(s) 467, 551, 598, 914, 1137, 1296
Cac8I GCNNGC 2 cut(s) 103, 1126
CaiI CAGNNNCTG 2 cut(s) 550, 629
CfoI GCGC 4 cut(s) 154, 198, 536, 1124
Cfr10I RCCGGY 2 cut(s) 148, 1253
Cfr13I GGNCC 6 cut(s) 50, 227, 248, 599, 677, 1220
ClaI ATCGAT 1 cut(s) 1208
CseI GACGC 2 cut(s) 436, 525
Csp6I GTAC 2 cut(s) 291, 732
CspAI ACCGGT 1 cut(s) 1253
CviAII CATG 8 cut(s) 443, 639, 700, 756, 946, 981, 1064, 1091
CviQI GTAC 2 cut(s) 291, 732
DdeI CTNAG 3 cut(s) 192, 665, 671
DinI GGCGCC 1 cut(s) 153
DpnI GATC 6 cut(s) 510, 590, 873, 966, 993, 1207
DpnII GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
DraIII CACNNNGTG 1 cut(s) 1082
DrdI GACNNNNNNGTC 1 cut(s) 1139
DriI GACNNNNNGTC 1 cut(s) 285
DseDI GACNNNNNNGTC 1 cut(s) 1139
Eam1105I GACNNNNNGTC 1 cut(s) 285
Eco130I CCWWGG 3 cut(s) 602, 699, 987
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 3 cut(s) 227, 248, 599
Eco57I CTGAAG 2 cut(s) 400, 957
EcoO109I RGGNCCY 3 cut(s) 248, 599, 1220
EcoRII CCWGG 3 cut(s) 549, 894, 1222
EcoT14I CCWWGG 3 cut(s) 602, 699, 987
EcoT22I ATGCAT 1 cut(s) 1092
EgeI GGCGCC 1 cut(s) 153
EheI GGCGCC 1 cut(s) 153
ErhI CCWWGG 3 cut(s) 602, 699, 987
FaeI CATG 8 cut(s) 446, 642, 703, 759, 949, 984, 1067, 1094
FaqI GGGAC 2 cut(s) 234, 283
FatI CATG 8 cut(s) 442, 638, 699, 755, 945, 980, 1063, 1090
FauI CCCGC 2 cut(s) 94, 260
FbaI TGATCA 1 cut(s) 588
FblI GTMKAC 2 cut(s) 9, 913
Fnu4HI GCNGC 7 cut(s) 155, 164, 213, 364, 431, 795, 1290
FokI GGATG 4 cut(s) 17, 193, 752, 1099
Fsp4HI GCNGC 7 cut(s) 155, 164, 213, 364, 431, 795, 1290
GlaI GCGC 4 cut(s) 153, 197, 535, 1123
GluI GCNGC 7 cut(s) 155, 164, 213, 364, 431, 795, 1290
GsaI CCCAGC 1 cut(s) 964
GsuI CTGGAG 1 cut(s) 910
HaeII RGCGCY 2 cut(s) 155, 199
HaeIII GGCC 4 cut(s) 52, 185, 679, 1221
HapII CCGG 2 cut(s) 149, 1254
HgaI GACGC 2 cut(s) 436, 525
HhaI GCGC 4 cut(s) 154, 198, 536, 1124
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 8 cut(s) 446, 642, 703, 759, 949, 984, 1067, 1094
Hin6I GCGC 4 cut(s) 152, 196, 534, 1122
HinP1I GCGC 4 cut(s) 152, 196, 534, 1122
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 4 cut(s) 241, 667, 1094, 1210
HpaII CCGG 2 cut(s) 149, 1254
HphI GGTGA 2 cut(s) 584, 1256
Hpy166II GTNNAC 4 cut(s) 10, 426, 734, 914
Hpy188I TCNGA 5 cut(s) 61, 484, 661, 1099, 1171
Hpy188III TCNNGA 1 cut(s) 889
Hpy8I GTNNAC 4 cut(s) 10, 426, 734, 914
Hpy99I CGWCG 3 cut(s) 163, 227, 283
HpyAV CCTTC 6 cut(s) 73, 217, 442, 483, 590, 885
HpyCH4III ACNGT 4 cut(s) 227, 517, 1119, 1141
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 9 cut(s) 111, 363, 391, 467, 797, 821, 907, 1090, 1128
HpyF3I CTNAG 3 cut(s) 192, 665, 671
HpySE526I ACGT 1 cut(s) 281
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 8 cut(s) 446, 642, 703, 759, 949, 984, 1067, 1094
HspAI GCGC 4 cut(s) 152, 196, 534, 1122
KasI GGCGCC 1 cut(s) 151
KflI GGGWCCC 1 cut(s) 248
Ksp22I TGATCA 1 cut(s) 588
Kzo9I GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
LmnI GCTCC 3 cut(s) 299, 842, 891
Lsp1109I GCAGC 5 cut(s) 175, 375, 442, 781, 1276
LweI GCATC 2 cut(s) 806, 1077
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 4 cut(s) 234, 772, 809, 1294
MalI GATC 6 cut(s) 510, 590, 873, 966, 993, 1207
MbiI CCGCTC 1 cut(s) 101
MboI GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
MboII GAAGA 3 cut(s) 267, 334, 1070
MflI RGATCY 1 cut(s) 871
MluCI AATT 4 cut(s) 340, 358, 778, 998
Mly113I GGCGCC 1 cut(s) 152
MlyI GAGTC 1 cut(s) 676
MmeI TCCRAC 2 cut(s) 84, 820
MnlI CCTC 7 cut(s) 8, 143, 196, 290, 430, 513, 1167
Mph1103I ATGCAT 1 cut(s) 1092
MseI TTAA 3 cut(s) 561, 765, 1001
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 3 cut(s) 215, 794, 1289
MspI CCGG 2 cut(s) 149, 1254
MspR9I CCNGG 3 cut(s) 551, 896, 1224
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 3 cut(s) 551, 896, 1224
MvnI CGCG 1 cut(s) 536
NarI GGCGCC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 699
NdeII GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
NlaIII CATG 8 cut(s) 446, 642, 703, 759, 949, 984, 1067, 1094
NlaIV GGNNCC 6 cut(s) 153, 249, 250, 873, 893, 1252
NmuCI GTSAC 3 cut(s) 234, 809, 1294
NsiI ATGCAT 1 cut(s) 1092
NspV TTCGAA 1 cut(s) 786
PctI GAATGC 1 cut(s) 391
PfeI GAWTC 3 cut(s) 241, 1094, 1210
PinAI ACCGGT 1 cut(s) 1253
PkrI GCNGC 7 cut(s) 156, 165, 214, 365, 432, 796, 1291
PleI GAGTC 1 cut(s) 675
PluTI GGCGCC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 675
PpuMI RGGWCCY 2 cut(s) 248, 599
PshBI ATTAAT 1 cut(s) 561
Psp5II RGGWCCY 2 cut(s) 248, 599
Psp6I CCWGG 3 cut(s) 549, 894, 1222
PspFI CCCAGC 1 cut(s) 960
PspGI CCWGG 3 cut(s) 549, 894, 1222
PspN4I GGNNCC 6 cut(s) 153, 249, 250, 873, 893, 1252
PspPI GGNCC 6 cut(s) 50, 227, 248, 599, 677, 1220
PspPPI RGGWCCY 2 cut(s) 248, 599
PstNI CAGNNNCTG 2 cut(s) 550, 629
PsuI RGATCY 1 cut(s) 871
PvuII CAGCTG 2 cut(s) 794, 1289
RsaI GTAC 2 cut(s) 292, 733
RsaNI GTAC 2 cut(s) 291, 732
RseI CAYNNNNRTG 1 cut(s) 704
SaqAI TTAA 3 cut(s) 561, 765, 1001
SatI GCNGC 7 cut(s) 155, 164, 213, 364, 431, 795, 1290
Sau3AI GATC 6 cut(s) 508, 588, 871, 964, 991, 1205
Sau96I GGNCC 6 cut(s) 50, 227, 248, 599, 677, 1220
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 3 cut(s) 551, 896, 1224
SfaNI GCATC 2 cut(s) 806, 1077
SfoI GGCGCC 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 786
SinI GGWCC 3 cut(s) 227, 248, 599
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 4 cut(s) 340, 358, 778, 998
SsiI CCGC 7 cut(s) 101, 155, 213, 267, 570, 1144, 1292
SspDI GGCGCC 1 cut(s) 151
SspI AATATT 1 cut(s) 1185
StyD4I CCNGG 3 cut(s) 549, 894, 1222
StyI CCWWGG 3 cut(s) 602, 699, 987
TaaI ACNGT 4 cut(s) 227, 517, 1119, 1141
TaiI ACGT 1 cut(s) 284
TaqI TCGA 5 cut(s) 188, 278, 786, 994, 1208
TasI AATT 4 cut(s) 340, 358, 778, 998
TauI GCSGC 2 cut(s) 157, 215
TfiI GAWTC 3 cut(s) 241, 1094, 1210
Tru1I TTAA 3 cut(s) 561, 765, 1001
Tru9I TTAA 3 cut(s) 561, 765, 1001
TscAI CASTG 6 cut(s) 474, 551, 598, 914, 1144, 1303
TseFI GTSAC 3 cut(s) 234, 809, 1294
TseI GCWGC 5 cut(s) 163, 363, 430, 794, 1289
Tsp45I GTSAC 3 cut(s) 234, 809, 1294
TspDTI ATGAA 1 cut(s) 819
TspGWI ACGGA 3 cut(s) 27, 253, 1070
TspRI CASTG 6 cut(s) 474, 551, 598, 914, 1144, 1303
VpaK11BI GGWCC 3 cut(s) 227, 248, 599
VspI ATTAAT 1 cut(s) 561
XmiI GTMKAC 2 cut(s) 9, 913
Zsp2I ATGCAT 1 cut(s) 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.