pycom12g06030

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001545
Physical Location & Seq
Forward (+)
6093909 .. 6099056
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06030.1

Sequence Viewer

Length: 807 bp
ATGGCTGATCCCATGACCACCAAAACCTCGTCAACTTTGCCCCCTCCACTGAAATACGAGGTTTTCGTGAGTTTTAGAGGTTTGGACACTCGGAAAGGTTTCACTGACCATTTGTACAATGCTCTAGTTCGAGACGGGATCCACACTTTCAGGGATGAAGAACAACTCGAGAGCGGAAAACCCATCTCAAAGGAACTCGCCAAAGCAATCGAAGAATCGCAAATTTTCGTTGTTGTTCTTTCCAGAAACTACGCAACCTCCACATGGTGCCTGGATGAACTTGCAAGTATGGTTGAACTTGCGGCCAACGATGAGTCTAGACTGATTCTGCCGGTATTCTATGACGTAACACCGTCGGAAGTCCGAAAACAGACCGGAGTTTGTTTTGAAGAGGCGTTTGCTAAACATGAAAAAGATTTCGAAGGAGAAACAGAGAAGGTGACAAGGTGGAAGAGTTCACTGACTACAATAGCCGAACTTTCAGGATTTGATCTAAGAAATTATAGATACGAGACACAACCGATTGGACAGATAGTTGAACGTATTTTCGGTGAATTGAATAACACGAAGCACACGTTCTCAGATGATCTAAAGGACTTCGTTGGTACGGTTCAAGTAGATAAAATCGAATCCATGAGTTCGTCTATAGGGTCGGAAGAAGTTTGTATGATTGGGATTTGTGGGATGCCAGGGGTTGGTAAGAGTACCATTGCAAAAGCTCTTTCTCAAAGAATTCGCAATCAATTTGAAGCTTTCAGTTTCATTTCTAAGAACAACTTTGTGTTCACTTGTTGGGTAGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

269

Amino Acids

30.29

Weight (kDa)

5.14

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 192 2.8e-47 TIR domain
TIR_2 PF13676 21 - 119 5.5e-19 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 695
AccBSI CCGCTC 1 cut(s) 174
AciI CCGC 2 cut(s) 174, 302
AclWI GGATC 3 cut(s) 2, 133, 146
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 2 cut(s) 222, 732
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 3 cut(s) 116, 607, 706
AfiI CCNNNNNNNGG 2 cut(s) 264, 695
AflIII ACRYGT 1 cut(s) 573
AgsI TTSAA 6 cut(s) 296, 389, 539, 559, 614, 749
AjnI CCWGG 2 cut(s) 270, 688
AluBI AGCT 2 cut(s) 719, 752
AluI AGCT 2 cut(s) 719, 752
Alw26I GTCTC 2 cut(s) 126, 506
AlwI GGATC 3 cut(s) 2, 133, 146
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 1 cut(s) 303
ApoI RAATTY 2 cut(s) 222, 732
Asp700I GAANNNNTTC 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 451, 563
AsuII TTCGAA 1 cut(s) 420
AvaI CYCGRG 1 cut(s) 167
BamHI GGATCC 1 cut(s) 138
BanI GGYRCC 1 cut(s) 267
BccI CCATC 1 cut(s) 191
BciT130I CCWGG 2 cut(s) 272, 690
BcoDI GTCTC 2 cut(s) 126, 506
BfaI CTAG 2 cut(s) 125, 318
BfmI CTRYAG 1 cut(s) 645
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 2 cut(s) 272, 690
BmeT110I CYCGRG 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 140, 269
BmrFI CCNGG 2 cut(s) 272, 690
BmsI GCATC 1 cut(s) 675
Bpu14I TTCGAA 1 cut(s) 420
BsaJI CCNNGG 1 cut(s) 689
BsaWI WCCGGW 1 cut(s) 374
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 695
Bse118I RCCGGY 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 2 cut(s) 272, 690
BseDI CCNNGG 1 cut(s) 689
BseGI GGATG 3 cut(s) 160, 280, 690
BseLI CCNNNNNNNGG 2 cut(s) 264, 695
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 1 cut(s) 594
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 2 cut(s) 332, 375
BslI CCNNNNNNNGG 2 cut(s) 264, 695
BsmAI GTCTC 2 cut(s) 126, 506
BsmBI CGTCTC 1 cut(s) 126
BsnI GGCC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 420
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 4 cut(s) 7, 138, 490, 586
BspACI CCGC 2 cut(s) 174, 302
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 1 cut(s) 593
BspLI GGNNCC 2 cut(s) 140, 269
BspPI GGATC 3 cut(s) 2, 133, 146
BspT104I TTCGAA 1 cut(s) 420
BspT107I GGYRCC 1 cut(s) 267
BsrBI CCGCTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 689
BssMI GATC 4 cut(s) 7, 138, 490, 586
Bst2UI CCWGG 2 cut(s) 272, 690
Bst4CI ACNGT 2 cut(s) 354, 610
Bst6I CTCTTC 2 cut(s) 384, 446
BstAUI TGTACA 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 420
BstDEI CTNAG 3 cut(s) 494, 580, 768
BstF5I GGATG 3 cut(s) 160, 280, 690
BstKTI GATC 4 cut(s) 10, 141, 493, 589
BstMAI GTCTC 2 cut(s) 126, 506
BstMBI GATC 4 cut(s) 7, 138, 490, 586
BstNI CCWGG 2 cut(s) 272, 690
BstSCI CCNGG 2 cut(s) 270, 688
BstSFI CTRYAG 1 cut(s) 645
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BsuRI GGCC 1 cut(s) 305
BtsCI GGATG 3 cut(s) 160, 280, 690
BtsIMutI CAGTG 3 cut(s) 47, 102, 458
Cfr10I RCCGGY 1 cut(s) 331
Csp6I GTAC 3 cut(s) 115, 606, 705
CviAII CATG 4 cut(s) 13, 264, 407, 634
CviJI RGCY 5 cut(s) 5, 305, 473, 719, 752
CviKI_1 RGCY 5 cut(s) 5, 305, 473, 719, 752
CviQI GTAC 3 cut(s) 115, 606, 705
DdeI CTNAG 3 cut(s) 494, 580, 768
DpnI GATC 4 cut(s) 9, 140, 492, 588
DpnII GATC 4 cut(s) 7, 138, 490, 586
DraIII CACNNNGTG 1 cut(s) 267
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 384, 446
EarI CTCTTC 2 cut(s) 384, 446
Eco88I CYCGRG 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 2 cut(s) 270, 688
Esp3I CGTCTC 1 cut(s) 126
FaeI CATG 4 cut(s) 16, 267, 410, 637
FaiI YATR 9 cut(s) 14, 265, 290, 342, 408, 504, 635, 647, 668
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FatI CATG 4 cut(s) 12, 263, 406, 633
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 3 cut(s) 167, 287, 697
Fsp4HI GCNGC 1 cut(s) 303
FspBI CTAG 2 cut(s) 125, 318
GluI GCNGC 1 cut(s) 303
HaeIII GGCC 1 cut(s) 305
HapII CCGG 2 cut(s) 332, 375
Hin1II CATG 4 cut(s) 16, 267, 410, 637
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 750
HinfI GANTC 4 cut(s) 215, 314, 325, 629
HpaII CCGG 2 cut(s) 332, 375
HphI GGTGA 2 cut(s) 451, 563
Hpy166II GTNNAC 3 cut(s) 33, 458, 786
Hpy188I TCNGA 5 cut(s) 93, 358, 365, 583, 655
Hpy188III TCNNGA 6 cut(s) 67, 131, 169, 243, 318, 483
Hpy8I GTNNAC 3 cut(s) 33, 458, 786
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 2 cut(s) 416, 430
HpyCH4III ACNGT 2 cut(s) 354, 610
HpyCH4IV ACGT 3 cut(s) 345, 541, 575
HpyCH4V TGCA 2 cut(s) 284, 713
HpyF3I CTNAG 3 cut(s) 494, 580, 768
HpySE526I ACGT 3 cut(s) 345, 541, 575
Hsp92II CATG 4 cut(s) 16, 267, 410, 637
Kzo9I GATC 4 cut(s) 7, 138, 490, 586
LpnPI CCDG 9 cut(s) 136, 256, 257, 284, 345, 388, 468, 675, 702
LweI GCATC 1 cut(s) 675
MaeI CTAG 2 cut(s) 125, 318
MaeII ACGT 3 cut(s) 345, 541, 575
MaeIII GTNAC 2 cut(s) 346, 439
MalI GATC 4 cut(s) 9, 140, 492, 588
MbiI CCGCTC 1 cut(s) 174
MboI GATC 4 cut(s) 7, 138, 490, 586
MboII GAAGA 5 cut(s) 170, 224, 401, 463, 668
MflI RGATCY 1 cut(s) 138
MluCI AATT 5 cut(s) 222, 499, 554, 732, 743
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 336, 633
MnlI CCTC 6 cut(s) 37, 52, 54, 71, 268, 385
MroXI GAANNNNTTC 1 cut(s) 98
MspI CCGG 2 cut(s) 332, 375
MspR9I CCNGG 2 cut(s) 272, 690
MvaI CCWGG 2 cut(s) 272, 690
NdeII GATC 4 cut(s) 7, 138, 490, 586
NlaIII CATG 4 cut(s) 16, 267, 410, 637
NlaIV GGNNCC 2 cut(s) 140, 269
NmuCI GTSAC 1 cut(s) 439
NspV TTCGAA 1 cut(s) 420
PaeR7I CTCGAG 1 cut(s) 167
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 572
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 215, 325, 629
PflMI CCANNNNNTGG 1 cut(s) 695
PkrI GCNGC 1 cut(s) 304
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Psp6I CCWGG 2 cut(s) 270, 688
PspGI CCWGG 2 cut(s) 270, 688
PspN4I GGNNCC 2 cut(s) 140, 269
PsuI RGATCY 1 cut(s) 138
RsaI GTAC 3 cut(s) 116, 607, 706
RsaNI GTAC 3 cut(s) 115, 606, 705
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 4 cut(s) 7, 138, 490, 586
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 2 cut(s) 272, 690
SfaNI GCATC 1 cut(s) 675
SfcI CTRYAG 1 cut(s) 645
Sfr274I CTCGAG 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 420
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 5 cut(s) 222, 499, 554, 732, 743
SsiI CCGC 2 cut(s) 174, 302
SspMI CTAG 2 cut(s) 125, 318
StyD4I CCNGG 2 cut(s) 270, 688
TaaI ACNGT 2 cut(s) 354, 610
TaiI ACGT 3 cut(s) 348, 544, 578
TaqI TCGA 5 cut(s) 130, 168, 210, 420, 627
TasI AATT 5 cut(s) 222, 499, 554, 732, 743
TatI WGTACW 1 cut(s) 114
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 3 cut(s) 215, 325, 629
TscAI CASTG 3 cut(s) 54, 109, 465
TseFI GTSAC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 4 cut(s) 171, 291, 423, 751
TspRI CASTG 3 cut(s) 54, 109, 465
Van91I CCANNNNNTGG 1 cut(s) 695
XapI RAATTY 2 cut(s) 222, 732
XbaI TCTAGA 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 125, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.