Rh1DG018600

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001545
Physical Location & Seq
Forward (+)
3032604 .. 3035108
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG018600.1

Sequence Viewer

Length: 1728 bp
ATGGGTGATTTTACAGTTGCCACGAAATTAGGTACTAGTGTGGGAGTTACAGCTATGATTATGAATTTGCTCGAGGCAGCCAAATCCAGTGAGGGCAGGTCATTGGACCAAATTCGTTTTCCATTGGGGCCTTTGAATTCCAACCCGAATAGTCTGACTGTTACACGAGGTTCTATCATCTTTCCTGCTGAGGGCTCGCCACATGAAAGAGAAAGAAGGCCCATTCATCAGGATGAAACAAATGCAATTGGTGATTTCAAGTTTCAGCTACCAGACTTTGGCCCTTCCTTTGACCGTTTCAATAACATGCGCTTGGATCAGAATCAGCTCCTGGGTTTTGACCGTTACTCGAAATACAATTCTTGTTTACCTCCTAATGAAATTGTAGAGTGGTTTGGGCATCCAAGCAGTGGGTCCTCGGTAACAATCCATCTACCACCATATCTGTATGATGACACCAATTGGACAGGACTAGCTTTATGCGCATACTTTTCAGTCCTCAACCACACAACTACCGACCTTGACAATTTGGATCTAGAAATTTCTCACAACCTTACATGTTTTCTTGAAACTGATAGAGGTTGTCTGGAGTCTCTTCATGGCTATTGCACCACCAACCAAGAATTCGAGTGGTTATATCATATGGGAGGATTCATTTGGTTGTCCTATATACCACGATGGTGGTTTTTAGATCAGTTGAATAAACGCAGCCACCTGGAGGCTTCAATTGGAAGCGACCGTGGAAGCTTGTGTGCGCATAGGTGTGGTCTCCGTCTTATGTATCTGCAAGATGAGGAAGGTTTTAAGCAGACCATAAGGCACTACATGGCCCCCTTGTCTGATATTGATCAAGGGAAAAATAAGCAATTCCAGGACTGCAAGGTGGGATCATCTCCTAGAACTGGCAGCTACATTGACTTTGATCGTTACTCGGTACATAATTTTTGTTTCCCTGCAACTGTAGCTCTAGAGTGGTTTGGGCATCAAAGCAATGGCTCCTCAGTAACAGTCCCACTACCACCGAACTTGCATAGTGATGCTAATTGGATAGGATTGACTTTATGTGCGTCCTTCTCAGTCGTGGAGCATCCAACTGCCGACCTTGAAAAATTACATTCAGGAAATCCTCACCACCTGATATGTCATTTGGAGTCAGAAAGAGGTAGTATAGAACCTCTCCATAACTATTGCACCACCAACGAAGAATTCCAGTGGTTGCATTTTGGAGGATTCATTTGGGTATCCTATATACCACGAGAGTGGTTTTTGGATCAGTTGAATGAATGCAGTGTCCTCGAGACTTCAATTGCAAGTGATCATGAAAGCTTTAGGGTGCATAACTGTGAGGTCCGTTGTGTATATCAGCATGATATGGAAGAGTTTAAGCAGAGCATATTGCACTGTATGACCTCGCTGTCTGGCAATAAAGGAGATGGCAAGCAATGCCCTGCAGGTGATGCAGGATCATCAAGCATTCCTAGCAGCTTCGTTGCGGAACCTCATCTTGAAAGATTAGAATGGCTGAATTACAAGAAAGGGCGTGATGAGGAAGAGTTTAAACAGACCCTAGTGCACTACATGGCGTTGTCGTCAGATAATAAAGGGAAAAATAAGCAATACCATCACGGTGAGACGGGATCATCGAGCAAAACAGACAACAGCATTGAGGAACTCCCTCACATTGAAAGAGTGCTCAATGATGTCAAGGGTAAACGAGTACTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

575

Amino Acids

65.33

Weight (kDa)

5.51

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 124 - 265 1e-10 C-JID domain
C-JID PF20160 324 - 459 2.1e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1442
AasI GACNNNNNNGTC 1 cut(s) 1414
Acc16I TGCGCA 2 cut(s) 484, 756
Acc36I ACCTGC 2 cut(s) 87, 1442
AccB7I CCANNNNNTGG 2 cut(s) 278, 410
AciI CCGC 1 cut(s) 1493
AclWI GGATC 6 cut(s) 324, 540, 895, 1278, 1471, 1645
AcsI RAATTY 6 cut(s) 64, 111, 136, 540, 623, 1205
AfaI GTAC 3 cut(s) 34, 936, 1719
AfiI CCNNNNNNNGG 5 cut(s) 191, 278, 410, 718, 902
AflIII ACRYGT 1 cut(s) 557
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 3 cut(s) 330, 714, 870
AjuI GAANNNNNNNTTGG 6 cut(s) 102, 134, 608, 640, 1190, 1222
AleI CACNNNNGTG 2 cut(s) 679, 1258
AluBI AGCT 9 cut(s) 53, 268, 328, 476, 747, 909, 965, 1326, 1485
AluI AGCT 9 cut(s) 53, 268, 328, 476, 747, 909, 965, 1326, 1485
Alw21I GWGCWC 2 cut(s) 1575, 1695
Alw26I GTCTC 4 cut(s) 597, 773, 1292, 1625
Alw44I GTGCAC 1 cut(s) 1571
AlwI GGATC 6 cut(s) 324, 540, 895, 1278, 1471, 1645
AlwNI CAGNNNCTG 1 cut(s) 331
Ama87I CYCGRG 2 cut(s) 71, 1295
AoxI GGCC 4 cut(s) 128, 218, 280, 828
ApaLI GTGCAC 1 cut(s) 1571
ApeKI GCWGC 4 cut(s) 77, 708, 906, 1482
ApoI RAATTY 6 cut(s) 64, 111, 136, 540, 623, 1205
AspLEI GCGC 3 cut(s) 312, 485, 757
AspS9I GGNCC 7 cut(s) 106, 128, 219, 281, 414, 829, 1348
AsuHPI GGTGA 5 cut(s) 17, 263, 1121, 1466, 1640
AvaI CYCGRG 2 cut(s) 71, 1295
AvaII GGWCC 3 cut(s) 106, 414, 1348
BaeGI GKGCMC 1 cut(s) 1575
BaeI ACNNNNGTAYC 2 cut(s) 24, 57
BanII GRGCYC 1 cut(s) 197
BauI CACGAG 2 cut(s) 165, 1254
Bbv12I GWGCWC 2 cut(s) 1575, 1695
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 89, 720, 918, 1494
BccI CCATC 4 cut(s) 438, 672, 1427, 1629
BcgI CGANNNNNNTGC 2 cut(s) 1276, 1310
BciT130I CCWGG 3 cut(s) 332, 716, 872
BciVI GTATCC 1 cut(s) 1252
BclI TGATCA 2 cut(s) 847, 1315
BcoDI GTCTC 4 cut(s) 597, 773, 1292, 1625
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 7 cut(s) 36, 473, 536, 897, 968, 1479, 1568
BfmI CTRYAG 2 cut(s) 960, 1449
BfuAI ACCTGC 2 cut(s) 87, 1442
BfuI GTATCC 1 cut(s) 1252
BisI GCNGC 4 cut(s) 78, 709, 907, 1483
BlsI GCNGC 4 cut(s) 79, 710, 908, 1484
BmcAI AGTACT 1 cut(s) 1719
Bme1390I CCNGG 3 cut(s) 332, 716, 872
Bme18I GGWCC 3 cut(s) 106, 414, 1348
BmeT110I CYCGRG 2 cut(s) 71, 1295
BmgT120I GGNCC 7 cut(s) 106, 128, 219, 281, 414, 829, 1348
BmiI GGNNCC 5 cut(s) 129, 415, 831, 997, 1497
BmrFI CCNGG 3 cut(s) 332, 716, 872
BmsI GCATC 5 cut(s) 409, 991, 1027, 1096, 1447
BpmI CTGGAG 2 cut(s) 608, 737
Bpu10I CCTNAGC 1 cut(s) 189
BsaBI GATNNNNATC 2 cut(s) 321, 846
BsaI GGTCTC 1 cut(s) 773
BsaJI CCNNGG 3 cut(s) 331, 417, 739
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 278, 410, 718, 902
Bse1I ACTGG 4 cut(s) 87, 907, 1210, 1725
Bse3DI GCAATG 2 cut(s) 997, 1448
Bse8I GATNNNNATC 2 cut(s) 321, 846
BseBI CCWGG 3 cut(s) 332, 716, 872
BseDI CCNNGG 3 cut(s) 331, 417, 739
BseGI GGATG 3 cut(s) 238, 400, 1087
BseJI GATNNNNATC 2 cut(s) 321, 846
BseLI CCNNNNNNNGG 5 cut(s) 191, 278, 410, 718, 902
BseMI GCAATG 2 cut(s) 997, 1448
BseMII CTCAG 3 cut(s) 180, 1014, 1089
BseNI ACTGG 4 cut(s) 87, 907, 1210, 1725
BseRI GAGGAG 1 cut(s) 988
BseSI GKGCMC 1 cut(s) 1575
BseXI GCAGC 4 cut(s) 89, 720, 918, 1494
Bsh1285I CGRYCG 1 cut(s) 739
BshFI GGCC 4 cut(s) 130, 220, 282, 830
BsiEI CGRYCG 1 cut(s) 739
BsiHKAI GWGCWC 2 cut(s) 1575, 1695
BsiHKCI CYCGRG 2 cut(s) 71, 1295
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 5 cut(s) 191, 278, 410, 718, 902
BsmAI GTCTC 4 cut(s) 597, 773, 1292, 1625
BsmBI CGTCTC 1 cut(s) 1625
BsmFI GGGAC 1 cut(s) 995
BsmI GAATGC 2 cut(s) 1289, 1473
BsnI GGCC 4 cut(s) 130, 220, 282, 830
Bso31I GGTCTC 1 cut(s) 773
BsoBI CYCGRG 2 cut(s) 71, 1295
Bsp1286I GDGCHC 3 cut(s) 197, 1575, 1695
BspACI CCGC 1 cut(s) 1493
BspANI GGCC 4 cut(s) 130, 220, 282, 830
BspCNI CTCAG 3 cut(s) 181, 1013, 1088
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 5 cut(s) 129, 415, 831, 997, 1497
BspMAI CTGCAG 1 cut(s) 1453
BspMI ACCTGC 2 cut(s) 87, 1442
BspPI GGATC 6 cut(s) 324, 540, 895, 1278, 1471, 1645
BspTNI GGTCTC 1 cut(s) 773
BsrDI GCAATG 2 cut(s) 997, 1448
BsrI ACTGG 4 cut(s) 87, 907, 1210, 1725
BssECI CCNNGG 3 cut(s) 331, 417, 739
BssSI CACGAG 2 cut(s) 165, 1254
Bst2BI CACGAG 2 cut(s) 165, 1254
Bst2UI CCWGG 3 cut(s) 332, 716, 872
Bst6I CTCTTC 3 cut(s) 600, 1371, 1545
BstAPI GCANNNNNTGC 2 cut(s) 1443, 1457
BstC8I GCNNGC 2 cut(s) 197, 1439
BstDEI CTNAG 3 cut(s) 189, 1000, 1075
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 3 cut(s) 238, 400, 1087
BstHHI GCGC 3 cut(s) 312, 485, 757
BstMAI GTCTC 4 cut(s) 597, 773, 1292, 1625
BstMCI CGRYCG 1 cut(s) 739
BstMWI GCNNNNNNNGC 5 cut(s) 482, 962, 1443, 1457, 1479
BstNI CCWGG 3 cut(s) 332, 716, 872
BstNSI RCATGY 2 cut(s) 310, 561
BstSCI CCNGG 3 cut(s) 330, 714, 870
BstSFI CTRYAG 2 cut(s) 960, 1449
BstSLI GKGCMC 1 cut(s) 1575
BstV1I GCAGC 4 cut(s) 89, 720, 918, 1494
BstX2I RGATCY 1 cut(s) 532
BstXI CCANNNNNNTGG 2 cut(s) 681, 1260
BstYI RGATCY 1 cut(s) 532
BsuI GTATCC 1 cut(s) 1252
BsuRI GGCC 4 cut(s) 130, 220, 282, 830
BtgI CCRYGG 1 cut(s) 739
BtsCI GGATG 3 cut(s) 238, 400, 1087
BtsI GCAGTG 2 cut(s) 415, 1294
BtsIMutI CAGTG 5 cut(s) 94, 415, 1217, 1294, 1399
BveI ACCTGC 2 cut(s) 87, 1442
Cac8I GCNNGC 2 cut(s) 197, 1439
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 1318
CfoI GCGC 3 cut(s) 312, 485, 757
Cfr13I GGNCC 7 cut(s) 106, 128, 219, 281, 414, 829, 1348
CseI GACGC 1 cut(s) 1056
Csp6I GTAC 3 cut(s) 33, 935, 1718
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviAII CATG 8 cut(s) 203, 307, 558, 599, 826, 1319, 1367, 1579
CviQI GTAC 3 cut(s) 33, 935, 1718
DdeI CTNAG 3 cut(s) 189, 1000, 1075
DraI TTTAAA 1 cut(s) 1558
DrdI GACNNNNNNGTC 1 cut(s) 1414
DseDI GACNNNNNNGTC 1 cut(s) 1414
Eam1104I CTCTTC 3 cut(s) 600, 1371, 1545
EarI CTCTTC 3 cut(s) 600, 1371, 1545
Eco24I GRGCYC 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 773
Eco47I GGWCC 3 cut(s) 106, 414, 1348
Eco88I CYCGRG 2 cut(s) 71, 1295
EcoO109I RGGNCCY 2 cut(s) 128, 414
EcoRI GAATTC 3 cut(s) 136, 623, 1205
EcoRII CCWGG 3 cut(s) 330, 714, 870
EcoT38I GRGCYC 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 1625
FaeI CATG 8 cut(s) 206, 310, 561, 602, 829, 1322, 1370, 1582
FaqI GGGAC 1 cut(s) 995
FatI CATG 8 cut(s) 202, 306, 557, 598, 825, 1318, 1366, 1578
FauNDI CATATG 1 cut(s) 642
FbaI TGATCA 2 cut(s) 847, 1315
Fnu4HI GCNGC 4 cut(s) 78, 709, 907, 1483
FokI GGATG 3 cut(s) 245, 387, 1074
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 4 cut(s) 78, 709, 907, 1483
FspAI RTGCGCAY 2 cut(s) 484, 756
FspBI CTAG 7 cut(s) 36, 473, 536, 897, 968, 1479, 1568
FspI TGCGCA 2 cut(s) 484, 756
GlaI GCGC 3 cut(s) 311, 484, 756
GluI GCNGC 4 cut(s) 78, 709, 907, 1483
GsuI CTGGAG 2 cut(s) 608, 737
HaeIII GGCC 4 cut(s) 130, 220, 282, 830
HgaI GACGC 1 cut(s) 1056
HhaI GCGC 3 cut(s) 312, 485, 757
Hin1II CATG 8 cut(s) 206, 310, 561, 602, 829, 1322, 1370, 1582
Hin6I GCGC 3 cut(s) 310, 483, 755
HinP1I GCGC 3 cut(s) 310, 483, 755
HindIII AAGCTT 2 cut(s) 745, 1324
HinfI GANTC 5 cut(s) 322, 590, 651, 1151, 1230
HphI GGTGA 5 cut(s) 17, 263, 1121, 1466, 1640
Hpy166II GTNNAC 3 cut(s) 368, 1573, 1712
Hpy188I TCNGA 5 cut(s) 156, 321, 841, 1156, 1594
Hpy188III TCNNGA 9 cut(s) 230, 536, 566, 587, 968, 1119, 1297, 1319, 1505
Hpy8I GTNNAC 3 cut(s) 368, 1573, 1712
HpyAV CCTTC 4 cut(s) 210, 294, 791, 1081
HpyF10VI GCNNNNNNNGC 5 cut(s) 482, 962, 1443, 1457, 1479
HpyF3I CTNAG 3 cut(s) 189, 1000, 1075
Hsp92II CATG 8 cut(s) 206, 310, 561, 602, 829, 1322, 1370, 1582
HspAI GCGC 3 cut(s) 310, 483, 755
Ksp22I TGATCA 2 cut(s) 847, 1315
LmnI GCTCC 3 cut(s) 333, 1001, 1084
Lsp1109I GCAGC 4 cut(s) 89, 720, 918, 1494
LweI GCATC 5 cut(s) 409, 991, 1027, 1096, 1447
MaeI CTAG 7 cut(s) 36, 473, 536, 897, 968, 1479, 1568
MaeIII GTNAC 6 cut(s) 46, 160, 344, 421, 926, 1003
MboII GAAGA 4 cut(s) 587, 1214, 1388, 1562
MfeI CAATTG 4 cut(s) 246, 460, 726, 1305
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 3 cut(s) 197, 1575, 1695
MlyI GAGTC 2 cut(s) 599, 1160
MmeI TCCRAC 2 cut(s) 165, 1115
MseI TTAA 3 cut(s) 804, 1383, 1557
MslI CAYNNNNRTG 7 cut(s) 231, 679, 762, 1035, 1258, 1341, 1626
MspR9I CCNGG 3 cut(s) 332, 716, 872
MssI GTTTAAAC 1 cut(s) 1558
MunI CAATTG 4 cut(s) 246, 460, 726, 1305
Mva1269I GAATGC 2 cut(s) 1289, 1473
MvaI CCWGG 3 cut(s) 332, 716, 872
MwoI GCNNNNNNNGC 5 cut(s) 482, 962, 1443, 1457, 1479
NdeI CATATG 1 cut(s) 642
NlaIII CATG 8 cut(s) 206, 310, 561, 602, 829, 1322, 1370, 1582
NlaIV GGNNCC 5 cut(s) 129, 415, 831, 997, 1497
NsbI TGCGCA 2 cut(s) 484, 756
NspI RCATGY 2 cut(s) 310, 561
OliI CACNNNNGTG 2 cut(s) 679, 1258
PaeR7I CTCGAG 2 cut(s) 71, 1295
PagI TCATGA 1 cut(s) 1318
PaqCI CACCTGC 1 cut(s) 1442
PciI ACATGT 1 cut(s) 557
PcsI WCGNNNNNNNCGW 1 cut(s) 1640
PctI GAATGC 2 cut(s) 1289, 1473
PfeI GAWTC 3 cut(s) 322, 651, 1230
PflMI CCANNNNNTGG 2 cut(s) 278, 410
PfoI TCCNGGA 1 cut(s) 870
PkrI GCNGC 4 cut(s) 79, 710, 908, 1484
PleI GAGTC 2 cut(s) 598, 1159
PmeI GTTTAAAC 1 cut(s) 1558
PpsI GAGTC 2 cut(s) 598, 1159
PpuMI RGGWCCY 1 cut(s) 414
PscI ACATGT 1 cut(s) 557
Psp5II RGGWCCY 1 cut(s) 414
Psp6I CCWGG 3 cut(s) 330, 714, 870
PspGI CCWGG 3 cut(s) 330, 714, 870
PspN4I GGNNCC 5 cut(s) 129, 415, 831, 997, 1497
PspPI GGNCC 7 cut(s) 106, 128, 219, 281, 414, 829, 1348
PspPPI RGGWCCY 1 cut(s) 414
PspXI VCTCGAGB 1 cut(s) 71
PstI CTGCAG 1 cut(s) 1453
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 532
RsaI GTAC 3 cut(s) 34, 936, 1719
RsaNI GTAC 3 cut(s) 33, 935, 1718
RseI CAYNNNNRTG 7 cut(s) 231, 679, 762, 1035, 1258, 1341, 1626
SaqAI TTAA 3 cut(s) 804, 1383, 1557
SatI GCNGC 4 cut(s) 78, 709, 907, 1483
Sau96I GGNCC 7 cut(s) 106, 128, 219, 281, 414, 829, 1348
SbfI CCTGCAGG 1 cut(s) 1453
ScaI AGTACT 1 cut(s) 1719
SchI GAGTC 2 cut(s) 599, 1160
ScrFI CCNGG 3 cut(s) 332, 716, 872
SdaI CCTGCAGG 1 cut(s) 1453
SduI GDGCHC 3 cut(s) 197, 1575, 1695
SfaNI GCATC 5 cut(s) 409, 991, 1027, 1096, 1447
SfcI CTRYAG 2 cut(s) 960, 1449
Sfr274I CTCGAG 2 cut(s) 71, 1295
SinI GGWCC 3 cut(s) 106, 414, 1348
SlaI CTCGAG 2 cut(s) 71, 1295
SmiMI CAYNNNNRTG 7 cut(s) 231, 679, 762, 1035, 1258, 1341, 1626
SmlI CTYRAG 2 cut(s) 71, 1295
SmoI CTYRAG 2 cut(s) 71, 1295
SpeI ACTAGT 1 cut(s) 35
Sse8387I CCTGCAGG 1 cut(s) 1453
SsiI CCGC 1 cut(s) 1493
SspMI CTAG 7 cut(s) 36, 473, 536, 897, 968, 1479, 1568
StyD4I CCNGG 3 cut(s) 330, 714, 870
TaqI TCGA 5 cut(s) 72, 350, 627, 1296, 1643
TatI WGTACW 1 cut(s) 1717
TfiI GAWTC 3 cut(s) 322, 651, 1230
Tru1I TTAA 3 cut(s) 804, 1383, 1557
Tru9I TTAA 3 cut(s) 804, 1383, 1557
TscAI CASTG 5 cut(s) 94, 415, 1217, 1294, 1406
TseI GCWGC 4 cut(s) 77, 708, 906, 1482
TspGWI ACGGA 2 cut(s) 761, 1340
TspRI CASTG 5 cut(s) 94, 415, 1217, 1294, 1406
Van91I CCANNNNNTGG 2 cut(s) 278, 410
VneI GTGCAC 1 cut(s) 1571
VpaK11BI GGWCC 3 cut(s) 106, 414, 1348
XapI RAATTY 6 cut(s) 64, 111, 136, 540, 623, 1205
XbaI TCTAGA 2 cut(s) 535, 967
XceI RCATGY 2 cut(s) 310, 561
XhoI CTCGAG 2 cut(s) 71, 1295
XspI CTAG 7 cut(s) 36, 473, 536, 897, 968, 1479, 1568
ZrmI AGTACT 1 cut(s) 1719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.