Rroxscaffold_4G00330060

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001545
Physical Location & Seq
Forward (+)
63425235 .. 63438366
13132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330060.1

Sequence Viewer

Length: 3444 bp
ATGATGTGGCCGTGTTCTTTGGCGTGGGAGATGATGTGGCAGCAGCTGTGGGTCGGTGGGCCTTCGATGAATGCCACAGACGACGACATATTGAGGTGGAGCGGAGGCGGTTATGGTGGTTCGGAGGCGGAGATTGTGCCGGAAGTTGCTGGGTCCCAACTCCCAAGAGGTGAAGAGCACGAAACAGAAGTGATTCAAAGGATAGTTGAACGCATTTTTGGTGCATTGAATTACACAAACCATATATTCTCTAATGATTTGAAGGACTTTGTTGGTATTGATCGTGTGAAAGAAATTGAATCGAACTTGTGTATGGAGTCGGAAGAAATTTGTGTAGTAGGGATTTGTGGCATGCCAGGGATTGGTAAGACAACCTTTGCACAAGCTCTTTTTGTAAGAATCCGCAATCAATTTGAAGCTTTTAGTTTCATTTCTAAGGTTGGAGAAATATGTAGGAGTGAAAGTTTATTTCATATCCAAGAGCAACTCTGTGATGACTTGTTGAATAGAAAAGTAAACATAAAGAATGTCAATGAAGTGATAAGCAGGAGATTGCATGGCAAACGAGTGCTTATCGTTCTTGACAACGTGGATGAATTAGAACAAATAGAGGCTGTAGCTGGCAATGGTGATAATTTGTATGATCGATTCGGTCCTGGTAGTAGAATCATCATAACTACAAGAGATGAAAGGTTGTTGATAAACTATGAGCCAAAAATATACAAGGTTGAGAAACTTACTGAAGATGAAGCTCTTCTGCTCTTCTGTCGGAAAGCATTTAAGAAAGACCATCCTTTGGATGGATTTGTTGAGTTGGCTTACAAATTTGTAGACTATATTGATGGTCTTCCTTTGGCTCTTGTAGTTTTGGGTTCCTCATTATTCAAAAGAAGTGTAAAAGAATGGTCTAGTAAATTTAACAGATTGAAAGATTACAATTATTCTGGTGAGAGGAAAATCATTGATATTCTTAAAGTCAGTTTTGATGGATTAGAGAATCAAGCAGAGAAGGAAATATTTTTAGACACTGCATGTTTCTTCAAAGGAGAGGATGCATGTCGTGTAGAAAAGATATTTGAGAGTTGTGGCTACTATCCAGACATCAGTATCAATATCCTCCTTGAGAAACATTTATTAAGTATTATAGGAGGAAAACTGTGGATGCATGATTTACTTCAAGAAATGGGTAGAGAAATTGTTCGAGGAGAGTCCCAAAAGCCAGGAAAGCGCAGCAGATTGTGGCTTCATACAGATGCCCTTCCTTTACTTGGGAATAATAAGGGGACAGATGCTGTCAAAGGTATTTTCTTAAGCTCGCCTCAACAAGACAATGTGCACTTGAAGGCAGACCCCTTCCCAAACATGGAAAGTCTAAGAATGTTGAAAATTTACAATGTGATATTTTCTGGATGCCTTGAATTTCTTTCAAATGAGTTAAGCTTCTTGGAATGGCACAGATATCCTTTAAAATCTCTGCCTTCATGTTTTGAACCTGATAAACTTGTTGAACTGAATTTGTATCTGAGTCAAATAGAGCAATTGTGGGAAGAAATTGAGAGGCCTTTGGAAAAGTTGGTGATAATCAACCTTAGTGACTGTGAATACTTGATCAAGACCCCTGACTTTGATAAAGTGCCAAAGCTTGAGAGGCTAATCCTCAAAGGTTGTACCAGATTGTCTGAGGTCGACCTCTCTGTTGGAGTGCTGCAACGGCTCATTTTGCTGAATCTGGAAGGTTGTGAATGCCTTACTACTCTTCTTCCCAACAGTATCAACTTGAGATCTCTCACAAAGTTCATTCTTTCGGGATGTTCCAAACTGAAAAATCTTCCAGAGTTTGGGGAAGAAATGAAACAATTAAGGGAACTTCACTTAAATGGGACAGCTATAGAAGAGCTACCAACATCAATGAAACATTTGACTGGCCTTACCCTGCTCAATCTCAAAGAATGCAAGAACCTTCTTTGTCTGCCAGAGGTCATTTGTAACGCTTTGACATCACTTCAAATATTGAACCTCTCAGGGTGTTCAAATCTTGCCTTGTTGCCGGAAAGCTTGGTGTGTTTAGAATACTTAGAGGAGCTTCATGCAGGTGGGAGTGCTATACCACAATTTCCTTCCTCCATTCTACATCTGAAAAACCTGCAAGTTTTATCTTTTGCTGGTTGTAAGGGACTGCCATCCTCTGTGGGTTTCGAGTTGCCTGCTTCATTTTCATCTGGTTTATGCTCTTTGAAGAAATTAAACCTTCGCAACTGCAATCTGTGTGAAGGATCAATCCCAGATGATCTTGGAAGCGTATCCTCTTTGCAGTATCTAGATTTAAGTGGAAACAATTTCATGACCATACCAAAAAGTATCTCTCAGCTTTCTCAGCTTGAAGACCTTGTATTGGATAATTGTAGCAACCTTCAATCATTGCCAAGACTTCCATCAAGTATAAGAGTTGTAACGGCACAGAATTGTCCTCTCCTACAGGGAACACATTCGAATAAATTAACGGTGTGGACTTCCACTGCTGCAGGATTTAGTGTTATAAATTGCCAAAGTGGTGAAGCCAAGCCTCAGCGGATTGACTTACCTGATCCACATCTTCGGAGGCCATTCTGTCAAACATTCTTCGAGGGTGCAATTTATCGCGGTAGTAATTTTGCATATAGTTATATATCAAAGGATACTCCTGCATGGTTGAGCCGTCGGAGTACTGGATCATCTATAACAATCCCACTACCTCCGGATTTGGATGATAACTGTAAATGGATGGGATTTGCTCTGTGTTTTACTTGTGTAACGCAGCATCATGATTCTCTGGATACAACATATGTCTTCCTTGATGACCAAACGCAAATAAACTTGAATCGCAATTACCGTATTCATTTCTATACAACGGAAGATCCTCTTGAACGTCCCCTAGTGCATAATTACCCTGATTGCGATTGGGTTGGATCATTTATGCACTGGAACTATACACCAAGAAGTGATTTTGTAGAAATTTCGAATAAACGCTTCATCCGAGCTACAGTTGTACCAGGCAGCCCAGAGGTGGAGGTGACAGGGTGTGCCGCGAGTCTAATATACTTGGAAGAAGTGGCAGAATTTGTCCAGAAAATGAATATTCACCATGACAATTGCAATAAAGGTTATAGGTATCTAGTGGATGCAATGGGAAGTATTCCTTCGACGAGCAGGATTCAAACTAAACCAGAAGTACAAAAACAAGAAACTACTAGTACTTCTGCCAGGCTTGTAGGACAATTGAGAAGAAACGTGGTGTCGTTGGTTGAAGAACTATTTGAGTGTCAATTTCATACATCTAATGAAGCCGTGGGTCCGAACCAATTGATACATCGCCTAGTGCTACGTTCCCCCTTCGATGACAATTTGATGGGGTCAAATCGACTCCTTCTTATTCATGTACCACGAGCACGTTTTCCGAACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1147

Amino Acids

129.68

Weight (kDa)

5.52

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 96 - 263 2.2e-20 NB-ARC domain
NACHT PF05729 113 - 260 1.5e-10 NACHT domain
WHD_ROQ1 PF23282 333 - 404 1.9e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 594 - 679 5.6e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 742 - 804 8.4e-07 Leucine-rich repeat region
LRR_8 PF13855 744 - 801 5.9e-06 Leucine rich repeat
C-JID PF20160 891 - 1029 1.2e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2537
AarI CACCTGC 1 cut(s) 2081
Acc36I ACCTGC 2 cut(s) 2081, 2151
AccB7I CCANNNNNTGG 3 cut(s) 362, 796, 1838
AccBSI CCGCTC 1 cut(s) 102
AccI GTMKAC 2 cut(s) 831, 1686
AccII CGCG 2 cut(s) 2640, 3065
AccIII TCCGGA 1 cut(s) 2734
AciI CCGC 7 cut(s) 102, 108, 128, 403, 2569, 2640, 3063
AclWI GGATC 5 cut(s) 2281, 2579, 2716, 2888, 2953
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 8 cut(s) 327, 824, 914, 1386, 1418, 1513, 2991, 3095
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 6 cut(s) 1669, 2704, 3027, 3210, 3232, 3417
AfiI CCNNNNNNNGG 8 cut(s) 362, 796, 800, 1268, 1363, 1838, 2392, 3043
AflII CTTAAG 1 cut(s) 1309
AhlI ACTAGT 1 cut(s) 3227
AjnI CCWGG 5 cut(s) 355, 655, 1219, 3028, 3239
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
Alw21I GWGCWC 3 cut(s) 180, 1338, 3427
Alw44I GTGCAC 1 cut(s) 1334
AlwI GGATC 5 cut(s) 2281, 2579, 2716, 2888, 2953
AlwNI CAGNNNCTG 2 cut(s) 46, 1292
Aor13HI TCCGGA 1 cut(s) 2734
AoxI GGCC 5 cut(s) 8, 59, 1559, 1924, 2600
ApaLI GTGCAC 1 cut(s) 1334
ApeKI GCWGC 7 cut(s) 40, 43, 1230, 1705, 2519, 2794, 3033
ApoI RAATTY 8 cut(s) 327, 824, 914, 1386, 1418, 1513, 2991, 3095
Asp700I GAANNNNTTC 4 cut(s) 192, 753, 1197, 2485
AspLEI GCGC 1 cut(s) 1230
AspS9I GGNCC 4 cut(s) 59, 153, 653, 3329
AsuHPI GGTGA 7 cut(s) 182, 641, 959, 1588, 2564, 3061, 3110
AsuII TTCGAA 2 cut(s) 2489, 2996
AvaII GGWCC 3 cut(s) 153, 653, 3329
BaeGI GKGCMC 1 cut(s) 1338
BaeI ACNNNNGTAYC 4 cut(s) 1090, 1123, 3009, 3042
BauI CACGAG 1 cut(s) 3420
BbsI GAAGAC 3 cut(s) 839, 2388, 2818
Bbv12I GWGCWC 3 cut(s) 180, 1338, 3427
BbvCI CCTCAGC 1 cut(s) 2565
BbvI GCAGC 7 cut(s) 52, 55, 1242, 1692, 2506, 2806, 3045
BccI CCATC 8 cut(s) 794, 798, 836, 980, 2188, 2440, 2755, 3379
BceAI ACGGC 4 cut(s) 1727, 2469, 2679, 3308
BcgI CGANNNNNNTGC 2 cut(s) 2186, 2220
BciT130I CCWGG 5 cut(s) 357, 657, 1221, 3030, 3241
BciVI GTATCC 3 cut(s) 2311, 2668, 2806
BclI TGATCA 1 cut(s) 1608
BcuI ACTAGT 1 cut(s) 3227
BfaI CTAG 6 cut(s) 909, 2318, 2912, 3152, 3228, 3353
BfmI CTRYAG 5 cut(s) 615, 1887, 2474, 2520, 3018
BfrI CTTAAG 1 cut(s) 1309
BfuAI ACCTGC 2 cut(s) 2081, 2151
BfuI GTATCC 3 cut(s) 2311, 2668, 2806
BglII AGATCT 1 cut(s) 1781
BisI GCNGC 8 cut(s) 41, 44, 1231, 1706, 2520, 2795, 3034, 3063
BlsI GCNGC 8 cut(s) 42, 45, 1232, 1707, 2521, 2796, 3035, 3064
BmcAI AGTACT 2 cut(s) 2704, 3232
Bme1390I CCNGG 5 cut(s) 357, 657, 1221, 3030, 3241
Bme18I GGWCC 3 cut(s) 153, 653, 3329
BmgT120I GGNCC 4 cut(s) 59, 153, 653, 3329
BmiI GGNNCC 4 cut(s) 154, 155, 874, 3330
BmrFI CCNGG 5 cut(s) 357, 657, 1221, 3030, 3241
BmsI GCATC 7 cut(s) 1042, 1152, 1243, 1279, 1400, 2806, 3148
BpiI GAAGAC 3 cut(s) 839, 2388, 2818
Bpu10I CCTNAGC 1 cut(s) 2565
Bpu14I TTCGAA 2 cut(s) 2489, 2996
BpuEI CTTGAG 3 cut(s) 1142, 1664, 1798
Bsa29I ATCGAT 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 2589
BsaJI CCNNGG 2 cut(s) 356, 3324
BsaWI WCCGGW 1 cut(s) 2734
BsaXI ACNNNNNCTCC 4 cut(s) 20, 50, 448, 478
Bsc4I CCNNNNNNNGG 8 cut(s) 362, 796, 800, 1268, 1363, 1838, 2392, 3043
Bse1I ACTGG 3 cut(s) 1927, 2710, 2963
Bse3DI GCAATG 3 cut(s) 631, 2417, 3168
Bse8I GATNNNNATC 1 cut(s) 2589
BseAI TCCGGA 1 cut(s) 2734
BseBI CCWGG 5 cut(s) 357, 657, 1221, 3030, 3241
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 2 cut(s) 356, 3324
BseJI GATNNNNATC 1 cut(s) 2589
BseLI CCNNNNNNNGG 8 cut(s) 362, 796, 800, 1268, 1363, 1838, 2392, 3043
BseMI GCAATG 3 cut(s) 631, 2417, 3168
BseMII CTCAG 6 cut(s) 1514, 1671, 2034, 2378, 2387, 2579
BseNI ACTGG 3 cut(s) 1927, 2710, 2963
BseRI GAGGAG 2 cut(s) 1218, 2093
BseSI GKGCMC 1 cut(s) 1338
BseXI GCAGC 7 cut(s) 52, 55, 1242, 1692, 2506, 2806, 3045
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 2 cut(s) 2640, 3065
BshFI GGCC 5 cut(s) 10, 61, 1561, 1926, 2602
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 3 cut(s) 180, 1338, 3427
BsiSI CCGG 3 cut(s) 140, 2048, 2735
BslFI GGGAC 6 cut(s) 139, 1195, 1297, 1894, 2187, 2892
BslI CCNNNNNNNGG 8 cut(s) 362, 796, 800, 1268, 1363, 1838, 2392, 3043
BsmFI GGGAC 6 cut(s) 139, 1195, 1297, 1894, 2187, 2892
BsmI GAATGC 3 cut(s) 76, 1748, 1955
BsnI GGCC 5 cut(s) 10, 61, 1561, 1926, 2602
Bsp119I TTCGAA 2 cut(s) 2489, 2996
Bsp1286I GDGCHC 3 cut(s) 180, 1338, 3427
Bsp13I TCCGGA 1 cut(s) 2734
BspACI CCGC 7 cut(s) 102, 108, 128, 403, 2569, 2640, 3063
BspANI GGCC 5 cut(s) 10, 61, 1561, 1926, 2602
BspCNI CTCAG 6 cut(s) 1515, 1672, 2033, 2377, 2386, 2578
BspDI ATCGAT 1 cut(s) 646
BspEI TCCGGA 1 cut(s) 2734
BspFNI CGCG 2 cut(s) 2640, 3065
BspHI TCATGA 2 cut(s) 2340, 2800
BspLI GGNNCC 4 cut(s) 154, 155, 874, 3330
BspMAI CTGCAG 1 cut(s) 2524
BspMI ACCTGC 2 cut(s) 2081, 2151
BspPI GGATC 5 cut(s) 2281, 2579, 2716, 2888, 2953
BspQI GCTCTTC 4 cut(s) 168, 759, 767, 1887
BspT104I TTCGAA 2 cut(s) 2489, 2996
BspTI CTTAAG 1 cut(s) 1309
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 3 cut(s) 631, 2417, 3168
BsrI ACTGG 3 cut(s) 1927, 2710, 2963
BssECI CCNNGG 2 cut(s) 356, 3324
BssSI CACGAG 1 cut(s) 3420
Bst2BI CACGAG 1 cut(s) 3420
Bst2UI CCWGG 5 cut(s) 357, 657, 1221, 3030, 3241
Bst4CI ACNGT 7 cut(s) 1158, 1598, 1769, 2503, 2753, 2870, 3022
Bst6I CTCTTC 5 cut(s) 168, 759, 767, 1761, 1887
BstAFI CTTAAG 1 cut(s) 1309
BstBI TTCGAA 2 cut(s) 2489, 2996
BstC8I GCNNGC 4 cut(s) 353, 622, 1316, 2205
BstDSI CCRYGG 1 cut(s) 3324
BstFNI CGCG 2 cut(s) 2640, 3065
BstHHI GCGC 1 cut(s) 1230
BstMWI GCNNNNNNNGC 5 cut(s) 1225, 1648, 1711, 1720, 2374
BstNI CCWGG 5 cut(s) 357, 657, 1221, 3030, 3241
BstNSI RCATGY 3 cut(s) 355, 1035, 1059
BstSCI CCNGG 5 cut(s) 355, 655, 1219, 3028, 3239
BstSFI CTRYAG 5 cut(s) 615, 1887, 2474, 2520, 3018
BstSLI GKGCMC 1 cut(s) 1338
BstUI CGCG 2 cut(s) 2640, 3065
BstV1I GCAGC 7 cut(s) 52, 55, 1242, 1692, 2506, 2806, 3045
BstV2I GAAGAC 3 cut(s) 839, 2388, 2818
BstX2I RGATCY 2 cut(s) 1781, 2893
BstYI RGATCY 2 cut(s) 1781, 2893
Bsu15I ATCGAT 1 cut(s) 646
BsuI GTATCC 3 cut(s) 2311, 2668, 2806
BsuRI GGCC 5 cut(s) 10, 61, 1561, 1926, 2602
BsuTUI ATCGAT 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 3324
BtgZI GCGATG 1 cut(s) 3332
BtsI GCAGTG 2 cut(s) 1026, 2514
BtsIMutI CAGTG 3 cut(s) 1026, 2514, 2956
BveI ACCTGC 2 cut(s) 2081, 2151
Cac8I GCNNGC 4 cut(s) 353, 622, 1316, 2205
CaiI CAGNNNCTG 2 cut(s) 46, 1292
CciI TCATGA 2 cut(s) 2340, 2800
CfoI GCGC 1 cut(s) 1230
Cfr13I GGNCC 4 cut(s) 59, 153, 653, 3329
ClaI ATCGAT 1 cut(s) 646
Csp6I GTAC 6 cut(s) 1668, 2703, 3026, 3209, 3231, 3416
CviQI GTAC 6 cut(s) 1668, 2703, 3026, 3209, 3231, 3416
DraI TTTAAA 1 cut(s) 1467
EaeI YGGCCR 1 cut(s) 8
Eam1104I CTCTTC 5 cut(s) 168, 759, 767, 1761, 1887
EarI CTCTTC 5 cut(s) 168, 759, 767, 1761, 1887
EciI GGCGGA 1 cut(s) 143
Eco147I AGGCCT 1 cut(s) 1561
Eco32I GATATC 1 cut(s) 1460
Eco47I GGWCC 3 cut(s) 153, 653, 3329
Eco57I CTGAAG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 5 cut(s) 355, 655, 1219, 3028, 3239
EcoRV GATATC 1 cut(s) 1460
EcoT22I ATGCAT 2 cut(s) 1057, 1167
FalI AAGNNNNNCTT 6 cut(s) 359, 391, 2883, 2915, 3160, 3192
FaqI GGGAC 6 cut(s) 139, 1195, 1297, 1894, 2187, 2892
FauNDI CATATG 1 cut(s) 2821
FbaI TGATCA 1 cut(s) 1608
FblI GTMKAC 2 cut(s) 831, 1686
Fnu4HI GCNGC 8 cut(s) 41, 44, 1231, 1706, 2520, 2795, 3034, 3063
Fsp4HI GCNGC 8 cut(s) 41, 44, 1231, 1706, 2520, 2795, 3034, 3063
FspBI CTAG 6 cut(s) 909, 2318, 2912, 3152, 3228, 3353
GlaI GCGC 1 cut(s) 1229
GluI GCNGC 8 cut(s) 41, 44, 1231, 1706, 2520, 2795, 3034, 3063
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 5 cut(s) 10, 61, 1561, 1926, 2602
HapII CCGG 3 cut(s) 140, 2048, 2735
HhaI GCGC 1 cut(s) 1230
Hin6I GCGC 1 cut(s) 1228
HinP1I GCGC 1 cut(s) 1228
HincII GTYRAC 1 cut(s) 1687
HindII GTYRAC 1 cut(s) 1687
HindIII AAGCTT 4 cut(s) 417, 1438, 1640, 2053
HpaII CCGG 3 cut(s) 140, 2048, 2735
HphI GGTGA 7 cut(s) 182, 641, 959, 1588, 2564, 3061, 3110
Hpy166II GTNNAC 5 cut(s) 517, 832, 1336, 1687, 2508
Hpy8I GTNNAC 5 cut(s) 517, 832, 1336, 1687, 2508
Hpy99I CGWCG 3 cut(s) 86, 2700, 3184
HpyCH4III ACNGT 7 cut(s) 1158, 1598, 1769, 2503, 2753, 2870, 3022
HpyCH4IV ACGT 5 cut(s) 588, 2905, 3267, 3361, 3427
HpyF10VI GCNNNNNNNGC 5 cut(s) 1225, 1648, 1711, 1720, 2374
HpySE526I ACGT 5 cut(s) 588, 2905, 3267, 3361, 3427
HspAI GCGC 1 cut(s) 1228
KflI GGGWCCC 1 cut(s) 153
Kpn2I TCCGGA 1 cut(s) 2734
Ksp22I TGATCA 1 cut(s) 1608
LguI GCTCTTC 4 cut(s) 168, 759, 767, 1887
LmnI GCTCC 2 cut(s) 99, 2080
Lsp1109I GCAGC 7 cut(s) 52, 55, 1242, 1692, 2506, 2806, 3045
LweI GCATC 7 cut(s) 1042, 1152, 1243, 1279, 1400, 2806, 3148
MaeI CTAG 6 cut(s) 909, 2318, 2912, 3152, 3228, 3353
MaeII ACGT 5 cut(s) 588, 2905, 3267, 3361, 3427
MaeIII GTNAC 5 cut(s) 1592, 1985, 2449, 2788, 3049
MbiI CCGCTC 1 cut(s) 102
MfeI CAATTG 4 cut(s) 1538, 3127, 3254, 3338
MflI RGATCY 2 cut(s) 1781, 2893
MhlI GDGCHC 3 cut(s) 180, 1338, 3427
MlyI GAGTC 5 cut(s) 326, 1217, 1534, 3076, 3393
MmeI TCCRAC 6 cut(s) 300, 421, 749, 1678, 2678, 2923
Mph1103I ATGCAT 2 cut(s) 1057, 1167
MroI TCCGGA 1 cut(s) 2734
MroXI GAANNNNTTC 4 cut(s) 192, 753, 1197, 2485
MslI CAYNNNNRTG 3 cut(s) 1251, 1875, 2091
MspA1I CMGCKG 2 cut(s) 46, 2569
MspCI CTTAAG 1 cut(s) 1309
MspI CCGG 3 cut(s) 140, 2048, 2735
MspR9I CCNGG 5 cut(s) 357, 657, 1221, 3030, 3241
MunI CAATTG 4 cut(s) 1538, 3127, 3254, 3338
Mva1269I GAATGC 3 cut(s) 76, 1748, 1955
MvaI CCWGG 5 cut(s) 357, 657, 1221, 3030, 3241
MvnI CGCG 2 cut(s) 2640, 3065
MwoI GCNNNNNNNGC 5 cut(s) 1225, 1648, 1711, 1720, 2374
NdeI CATATG 1 cut(s) 2821
NlaIV GGNNCC 4 cut(s) 154, 155, 874, 3330
NmuCI GTSAC 2 cut(s) 1592, 3049
NsiI ATGCAT 2 cut(s) 1057, 1167
NspI RCATGY 3 cut(s) 355, 1035, 1059
NspV TTCGAA 2 cut(s) 2489, 2996
PaeI GCATGC 1 cut(s) 355
PagI TCATGA 2 cut(s) 2340, 2800
PaqCI CACCTGC 1 cut(s) 2081
PceI AGGCCT 1 cut(s) 1561
PciSI GCTCTTC 4 cut(s) 168, 759, 767, 1887
PcsI WCGNNNNNNNCGW 2 cut(s) 2866, 3010
PctI GAATGC 3 cut(s) 76, 1748, 1955
PdmI GAANNNNTTC 4 cut(s) 192, 753, 1197, 2485
PflMI CCANNNNNTGG 3 cut(s) 362, 796, 1838
PkrI GCNGC 8 cut(s) 42, 45, 1232, 1707, 2521, 2796, 3035, 3064
PleI GAGTC 5 cut(s) 325, 1216, 1533, 3075, 3393
PpsI GAGTC 5 cut(s) 325, 1216, 1533, 3075, 3393
PpuMI RGGWCCY 1 cut(s) 153
PsiI TTATAA 1 cut(s) 2537
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 5 cut(s) 355, 655, 1219, 3028, 3239
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 5 cut(s) 355, 655, 1219, 3028, 3239
PspN4I GGNNCC 4 cut(s) 154, 155, 874, 3330
PspPI GGNCC 4 cut(s) 59, 153, 653, 3329
PspPPI RGGWCCY 1 cut(s) 153
PstI CTGCAG 1 cut(s) 2524
PstNI CAGNNNCTG 2 cut(s) 46, 1292
PsuI RGATCY 2 cut(s) 1781, 2893
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 6 cut(s) 1669, 2704, 3027, 3210, 3232, 3417
RsaNI GTAC 6 cut(s) 1668, 2703, 3026, 3209, 3231, 3416
RseI CAYNNNNRTG 3 cut(s) 1251, 1875, 2091
SalI GTCGAC 1 cut(s) 1685
SapI GCTCTTC 4 cut(s) 168, 759, 767, 1887
SatI GCNGC 8 cut(s) 41, 44, 1231, 1706, 2520, 2795, 3034, 3063
Sau96I GGNCC 4 cut(s) 59, 153, 653, 3329
ScaI AGTACT 2 cut(s) 2704, 3232
SchI GAGTC 5 cut(s) 326, 1217, 1534, 3076, 3393
ScrFI CCNGG 5 cut(s) 357, 657, 1221, 3030, 3241
SduI GDGCHC 3 cut(s) 180, 1338, 3427
SfaNI GCATC 7 cut(s) 1042, 1152, 1243, 1279, 1400, 2806, 3148
SfcI CTRYAG 5 cut(s) 615, 1887, 2474, 2520, 3018
SfuI TTCGAA 2 cut(s) 2489, 2996
SinI GGWCC 3 cut(s) 153, 653, 3329
SmiMI CAYNNNNRTG 3 cut(s) 1251, 1875, 2091
SmlI CTYRAG 4 cut(s) 1121, 1309, 1643, 1777
SmoI CTYRAG 4 cut(s) 1121, 1309, 1643, 1777
SpeI ACTAGT 1 cut(s) 3227
SphI GCATGC 1 cut(s) 355
SseBI AGGCCT 1 cut(s) 1561
SsiI CCGC 7 cut(s) 102, 108, 128, 403, 2569, 2640, 3063
SspI AATATT 3 cut(s) 1017, 2010, 3115
SspMI CTAG 6 cut(s) 909, 2318, 2912, 3152, 3228, 3353
StuI AGGCCT 1 cut(s) 1561
StyD4I CCNGG 5 cut(s) 355, 655, 1219, 3028, 3239
TaaI ACNGT 7 cut(s) 1158, 1598, 1769, 2503, 2753, 2870, 3022
TaiI ACGT 5 cut(s) 591, 2908, 3270, 3364, 3430
TaqII GACCGA 1 cut(s) 641
TatI WGTACW 3 cut(s) 2702, 3208, 3230
TauI GCSGC 1 cut(s) 3065
TscAI CASTG 3 cut(s) 1033, 2521, 2963
TseFI GTSAC 2 cut(s) 1592, 3049
TseI GCWGC 7 cut(s) 40, 43, 1230, 1705, 2519, 2794, 3033
Tsp45I GTSAC 2 cut(s) 1592, 3049
TspGWI ACGGA 1 cut(s) 2903
TspRI CASTG 3 cut(s) 1033, 2521, 2963
Van91I CCANNNNNTGG 3 cut(s) 362, 796, 1838
Vha464I CTTAAG 1 cut(s) 1309
VneI GTGCAC 1 cut(s) 1334
VpaK11BI GGWCC 3 cut(s) 153, 653, 3329
XapI RAATTY 8 cut(s) 327, 824, 914, 1386, 1418, 1513, 2991, 3095
XbaI TCTAGA 1 cut(s) 2317
XceI RCATGY 3 cut(s) 355, 1035, 1059
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmiI GTMKAC 2 cut(s) 831, 1686
XmnI GAANNNNTTC 4 cut(s) 192, 753, 1197, 2485
XspI CTAG 6 cut(s) 909, 2318, 2912, 3152, 3228, 3353
ZrmI AGTACT 2 cut(s) 2704, 3232
Zsp2I ATGCAT 2 cut(s) 1057, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.