Rh4DG296900

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001545
Physical Location & Seq
Forward (+)
51872714 .. 51874499
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG296900.1

Sequence Viewer

Length: 1200 bp
ATGCCGCTACCACCTAATCTAGATGGAGACGCTAATTGGATAGGATTGGCTTTCTACGTGTCCTTTTCAGTCCTGGAGAATGCAGTTGCCGACCTTGCCAACAATTTGGCCCCCGGAATTTCTCACCACCTTACATGTTTTTTTGAAACAAGCAAAGGTAGTCTACAATCTCTCCATGACTATTGCACCACCAACAAAGAATTCCAGTGGTTGCATCTAGGAGGATTCATTTGGCTCTCCTATATACTGCGAAAATGGTTTGCAGATCAGCTGAATGAATGTGGTCGCCTGGAGGCTTCCATTGTAAGTGGTCATGGAGCATTCAGCGTGGAGAAGTGTGGATTCCGGCTTGTATATCAACAAGATGAGGAAGAGCTTAAGCAGACCATAATGCTTTGCATGACCTCTTTGCCAGATAATGATATAAAAAAGCTAGACCATGATGGAGAGGCAGCTGGAACTAGTGGAGTACAATCGGTCGTTTATGATCAGCTCGGTGGAGCCAGTACTACAATTGGTAACAATGACAAGGAGTTTGACCGTGGCTCGGTATACAACTTTTGTTTCCCTCCAAATGATATCGTAGAGTGGTTTGGGCATCACAACGATGGCCCCTCAGTGACAATGCTGTTACCATCAAATCTATATGGAGACGCTACTCCTAATTGGATAGGAGTGGCTTTCTGTGCATACTTTGAAGTCCTGGATCGCCTAGCTACCAACCTTGACAATTCGGATCCTGGAGTTCCTCACCACCTTACTTGTTATTTTGAAACAGATGGAGGTAGTCTGGAATCAATCCATGACTATTCAACCTTCAAGAATGAGTTCCAGTGGTTGCATCTAGGAGGATTCATTTGGCTCTCCTACATACCACGCGCATGGTTTTTAGACCAGTTGAATGAATGCGGTAAGCTGGAAGCTTCAATCACAAGCGATCACCAAACCTTTAATGTGCGCAAGTGTGGTATCCATCGTGTATATCAGCATGATGTGGAGGAGTTTAAGAAGACCATAATGCACTGCATAACCTCTTTGTCTGAGCATGACAACAGAAAGAATAAGCAATACCCTGAAATCGGCGGCGAGGCTGGATCATCGGGTAGAACTGGATGCAACAATATTGTCATTGAGGACCCTCAAAATGGAAGTTTGGAAGGACCCCTAACTGATAGGAAGGGTAAAGGAGTGCTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

399

Amino Acids

44.61

Weight (kDa)

4.96

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 190 - 333 1.1e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 959
AccI GTMKAC 2 cut(s) 163, 552
AccII CGCG 1 cut(s) 879
AciI CCGC 3 cut(s) 5, 909, 1083
AclWI GGATC 4 cut(s) 714, 731, 744, 1102
AcsI RAATTY 2 cut(s) 117, 200
AfaI GTAC 2 cut(s) 471, 508
AfiI CCNNNNNNNGG 3 cut(s) 547, 1079, 1145
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 2 cut(s) 57, 134
AgsI TTSAA 7 cut(s) 146, 698, 773, 813, 820, 901, 927
AhlI ACTAGT 1 cut(s) 461
AjnI CCWGG 4 cut(s) 72, 288, 702, 739
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 8 cut(s) 271, 376, 433, 455, 493, 716, 916, 923
AluI AGCT 8 cut(s) 271, 376, 433, 455, 493, 716, 916, 923
Alw26I GTCTC 2 cut(s) 21, 645
AlwI GGATC 4 cut(s) 714, 731, 744, 1102
AoxI GGCC 2 cut(s) 108, 610
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 2 cut(s) 117, 200
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp700I GAANNNNTTC 1 cut(s) 827
AspLEI GCGC 2 cut(s) 881, 960
AspS9I GGNCC 4 cut(s) 109, 611, 1135, 1160
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 116, 743, 932
AvaII GGWCC 2 cut(s) 1135, 1160
BamHI GGATCC 1 cut(s) 736
BbsI GAAGAC 1 cut(s) 1016
BbvI GCAGC 1 cut(s) 464
BccI CCATC 6 cut(s) 17, 437, 602, 643, 773, 981
BciT130I CCWGG 4 cut(s) 74, 290, 704, 741
BciVI GTATCC 1 cut(s) 980
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 21, 645
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 6 cut(s) 20, 218, 434, 462, 713, 845
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 980
BisI GCNGC 3 cut(s) 5, 453, 1084
BlsI GCNGC 3 cut(s) 6, 454, 1085
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 5 cut(s) 74, 114, 290, 704, 741
Bme18I GGWCC 2 cut(s) 1135, 1160
BmgT120I GGNCC 4 cut(s) 109, 611, 1135, 1160
BmiI GGNNCC 6 cut(s) 111, 502, 613, 738, 1137, 1162
BmrFI CCNGG 5 cut(s) 74, 114, 290, 704, 741
BmsI GCATC 4 cut(s) 223, 607, 850, 1103
BpiI GAAGAC 1 cut(s) 1016
BpmI CTGGAG 3 cut(s) 95, 311, 762
BpuMI CCSGG 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 2 cut(s) 112, 541
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 1079, 1145
Bse1I ACTGG 5 cut(s) 205, 504, 832, 895, 1114
BseBI CCWGG 4 cut(s) 74, 290, 704, 741
BseDI CCNNGG 2 cut(s) 112, 541
BseGI GGATG 1 cut(s) 1118
BseLI CCNNNNNNNGG 3 cut(s) 547, 1079, 1145
BseMII CTCAG 2 cut(s) 630, 1032
BseNI ACTGG 5 cut(s) 205, 504, 832, 895, 1114
BseRI GAGGAG 1 cut(s) 1013
BseXI GCAGC 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 879
Bsh1285I CGRYCG 1 cut(s) 480
BshFI GGCC 2 cut(s) 110, 612
BsiEI CGRYCG 1 cut(s) 480
BsiSI CCGG 2 cut(s) 114, 346
BslI CCNNNNNNNGG 3 cut(s) 547, 1079, 1145
BsmAI GTCTC 2 cut(s) 21, 645
BsmBI CGTCTC 2 cut(s) 21, 645
BsmI GAATGC 3 cut(s) 85, 320, 911
BsnI GGCC 2 cut(s) 110, 612
Bsp143I GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
BspACI CCGC 3 cut(s) 5, 909, 1083
BspANI GGCC 2 cut(s) 110, 612
BspCNI CTCAG 2 cut(s) 629, 1033
BspFNI CGCG 1 cut(s) 879
BspLI GGNNCC 6 cut(s) 111, 502, 613, 738, 1137, 1162
BspPI GGATC 4 cut(s) 714, 731, 744, 1102
BspQI GCTCTTC 1 cut(s) 366
BspTI CTTAAG 1 cut(s) 377
BsrI ACTGG 5 cut(s) 205, 504, 832, 895, 1114
BssECI CCNNGG 2 cut(s) 112, 541
BssMI GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
BssNAI GTATAC 1 cut(s) 553
Bst1107I GTATAC 1 cut(s) 553
Bst2UI CCWGG 4 cut(s) 74, 290, 704, 741
Bst4CI ACNGT 1 cut(s) 542
Bst6I CTCTTC 1 cut(s) 366
BstAFI CTTAAG 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 616, 1041
BstDSI CCRYGG 1 cut(s) 541
BstF5I GGATG 1 cut(s) 1118
BstFNI CGCG 1 cut(s) 879
BstHHI GCGC 2 cut(s) 881, 960
BstKTI GATC 6 cut(s) 268, 490, 709, 739, 940, 1097
BstMAI GTCTC 2 cut(s) 21, 645
BstMBI GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
BstMCI CGRYCG 1 cut(s) 480
BstMWI GCNNNNNNNGC 2 cut(s) 95, 686
BstNI CCWGG 4 cut(s) 74, 290, 704, 741
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 5 cut(s) 72, 112, 288, 702, 739
BstUI CGCG 1 cut(s) 879
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 1016
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 2 cut(s) 106, 882
BstYI RGATCY 1 cut(s) 736
BstZ17I GTATAC 1 cut(s) 553
BsuI GTATCC 1 cut(s) 980
BsuRI GGCC 2 cut(s) 110, 612
BtgI CCRYGG 1 cut(s) 541
BtsCI GGATG 1 cut(s) 1118
BtsI GCAGTG 1 cut(s) 1021
BtsIMutI CAGTG 4 cut(s) 212, 624, 839, 1021
CfoI GCGC 2 cut(s) 881, 960
Cfr13I GGNCC 4 cut(s) 109, 611, 1135, 1160
CseI GACGC 2 cut(s) 38, 662
Csp6I GTAC 2 cut(s) 470, 507
CspCI CAANNNNNGTGG 2 cut(s) 743, 778
CviAII CATG 9 cut(s) 135, 176, 314, 400, 440, 803, 882, 989, 1046
CviQI GTAC 2 cut(s) 470, 507
DdeI CTNAG 2 cut(s) 616, 1041
DpnI GATC 6 cut(s) 267, 489, 708, 738, 939, 1096
DpnII GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 1135, 1160
EcoO109I RGGNCCY 2 cut(s) 1135, 1160
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 4 cut(s) 72, 288, 702, 739
EcoRV GATATC 1 cut(s) 580
Esp3I CGTCTC 2 cut(s) 21, 645
FaeI CATG 9 cut(s) 138, 179, 317, 403, 443, 806, 885, 992, 1049
FatI CATG 9 cut(s) 134, 175, 313, 399, 439, 802, 881, 988, 1045
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 2 cut(s) 163, 552
Fnu4HI GCNGC 3 cut(s) 5, 453, 1084
FokI GGATG 1 cut(s) 1125
Fsp4HI GCNGC 3 cut(s) 5, 453, 1084
FspBI CTAG 6 cut(s) 20, 218, 434, 462, 713, 845
FspI TGCGCA 1 cut(s) 959
GlaI GCGC 2 cut(s) 880, 959
GluI GCNGC 3 cut(s) 5, 453, 1084
GsuI CTGGAG 3 cut(s) 95, 311, 762
HaeIII GGCC 2 cut(s) 110, 612
HapII CCGG 2 cut(s) 114, 346
HgaI GACGC 2 cut(s) 38, 662
HhaI GCGC 2 cut(s) 881, 960
Hin1II CATG 9 cut(s) 138, 179, 317, 403, 443, 806, 885, 992, 1049
Hin6I GCGC 2 cut(s) 879, 958
HinP1I GCGC 2 cut(s) 879, 958
HindIII AAGCTT 1 cut(s) 921
HinfI GANTC 4 cut(s) 225, 342, 794, 852
HpaII CCGG 2 cut(s) 114, 346
HphI GGTGA 3 cut(s) 116, 743, 932
Hpy166II GTNNAC 2 cut(s) 164, 553
Hpy188I TCNGA 2 cut(s) 736, 1042
Hpy188III TCNNGA 3 cut(s) 20, 791, 820
Hpy8I GTNNAC 2 cut(s) 164, 553
HpyAV CCTTC 3 cut(s) 826, 1151, 1171
HpyCH4III ACNGT 1 cut(s) 542
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 686
HpyF3I CTNAG 2 cut(s) 616, 1041
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 9 cut(s) 138, 179, 317, 403, 443, 806, 885, 992, 1049
HspAI GCGC 2 cut(s) 879, 958
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 317, 500
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 4 cut(s) 223, 607, 850, 1103
MaeI CTAG 6 cut(s) 20, 218, 434, 462, 713, 845
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 3 cut(s) 518, 619, 630
MalI GATC 6 cut(s) 267, 489, 708, 738, 939, 1096
MboI GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
MboII GAAGA 2 cut(s) 383, 1021
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 1 cut(s) 736
MluCI AATT 7 cut(s) 34, 103, 117, 200, 513, 664, 730
MroXI GAANNNNTTC 1 cut(s) 827
MseI TTAA 3 cut(s) 378, 951, 1005
MslI CAYNNNNRTG 2 cut(s) 606, 880
MspA1I CMGCKG 2 cut(s) 271, 455
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 2 cut(s) 114, 346
MspR9I CCNGG 5 cut(s) 74, 114, 290, 704, 741
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 3 cut(s) 85, 320, 911
MvaI CCWGG 4 cut(s) 74, 290, 704, 741
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 2 cut(s) 95, 686
NciI CCSGG 1 cut(s) 114
NdeII GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
NlaIII CATG 9 cut(s) 138, 179, 317, 403, 443, 806, 885, 992, 1049
NlaIV GGNNCC 6 cut(s) 111, 502, 613, 738, 1137, 1162
NmuCI GTSAC 1 cut(s) 619
NsbI TGCGCA 1 cut(s) 959
NspI RCATGY 1 cut(s) 138
PciI ACATGT 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 366
PctI GAATGC 3 cut(s) 85, 320, 911
PdmI GAANNNNTTC 1 cut(s) 827
PfeI GAWTC 4 cut(s) 225, 342, 794, 852
PfoI TCCNGGA 3 cut(s) 72, 702, 739
PkrI GCNGC 3 cut(s) 6, 454, 1085
Ppu21I YACGTR 1 cut(s) 58
PpuMI RGGWCCY 2 cut(s) 1135, 1160
PscI ACATGT 1 cut(s) 134
Psp5II RGGWCCY 2 cut(s) 1135, 1160
Psp6I CCWGG 4 cut(s) 72, 288, 702, 739
PspGI CCWGG 4 cut(s) 72, 288, 702, 739
PspN4I GGNNCC 6 cut(s) 111, 502, 613, 738, 1137, 1162
PspPI GGNCC 4 cut(s) 109, 611, 1135, 1160
PspPPI RGGWCCY 2 cut(s) 1135, 1160
PsuI RGATCY 1 cut(s) 736
PvuII CAGCTG 2 cut(s) 271, 455
RsaI GTAC 2 cut(s) 471, 508
RsaNI GTAC 2 cut(s) 470, 507
RseI CAYNNNNRTG 2 cut(s) 606, 880
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 3 cut(s) 378, 951, 1005
SatI GCNGC 3 cut(s) 5, 453, 1084
Sau3AI GATC 6 cut(s) 265, 487, 706, 736, 937, 1094
Sau96I GGNCC 4 cut(s) 109, 611, 1135, 1160
ScaI AGTACT 1 cut(s) 508
ScrFI CCNGG 5 cut(s) 74, 114, 290, 704, 741
SfaNI GCATC 4 cut(s) 223, 607, 850, 1103
SinI GGWCC 2 cut(s) 1135, 1160
SmiMI CAYNNNNRTG 2 cut(s) 606, 880
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 7 cut(s) 34, 103, 117, 200, 513, 664, 730
SsiI CCGC 3 cut(s) 5, 909, 1083
SspI AATATT 1 cut(s) 1123
SspMI CTAG 6 cut(s) 20, 218, 434, 462, 713, 845
StyD4I CCNGG 5 cut(s) 72, 112, 288, 702, 739
TaaI ACNGT 1 cut(s) 542
TaiI ACGT 1 cut(s) 60
TaqII GACCGA 1 cut(s) 466
TasI AATT 7 cut(s) 34, 103, 117, 200, 513, 664, 730
TatI WGTACW 2 cut(s) 469, 506
TauI GCSGC 2 cut(s) 7, 1086
TfiI GAWTC 4 cut(s) 225, 342, 794, 852
Tru1I TTAA 3 cut(s) 378, 951, 1005
Tru9I TTAA 3 cut(s) 378, 951, 1005
TscAI CASTG 4 cut(s) 212, 624, 839, 1028
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 4 cut(s) 217, 291, 844, 918
TspRI CASTG 4 cut(s) 212, 624, 839, 1028
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 1135, 1160
XapI RAATTY 2 cut(s) 117, 200
XbaI TCTAGA 1 cut(s) 19
XceI RCATGY 1 cut(s) 138
XmiI GTMKAC 2 cut(s) 163, 552
XmnI GAANNNNTTC 1 cut(s) 827
XspI CTAG 6 cut(s) 20, 218, 434, 462, 713, 845
ZrmI AGTACT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.