Rh4AG293800

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001545
Physical Location & Seq
Forward (+)
60742239 .. 60751431
9193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG293800.1

Sequence Viewer

Length: 1176 bp
ATGGAGCTATTTTCTCAGGGTGGTTGTTTTCAATTTTTTTTGGGGCTGAAACTGATATTGCTTCATGATCCTCCCCTAAAGTATCCACTCGCGGATCGCGATGGTCGTGAGACATATCAGCATTTGCTTTATATACCAGCAGATCATTTCGTGAAAAGGTTGAATAAATGCTTCATCGAGGCTAAAATCAACATCAAAAGAACAGGGGTGGAGGTGGTAGCATGTAGGGCGAGGCTGTTGTACTCGGCAGAAGTGCCAGAATTTGTCGGGACTCTCGTTCGCCTTTGGAATCATGAACAAGTGGGGCATCTGGCAAATCCATCATCGAGCAAGTGCACTGATCAGTCAAAAGTTTATCATTTTGCTTCAGAAACAAGTTTCTTGATTGAAATTGAACGCCAGATTTTCAGAAATTTAAGTTCAACCAGGATGGAAGGTCATGAAGTGAGAACAAGGGTAGAGGCATTGCTGCTTGAAGAAATATTTGAGCTGGAATCCGCACGTAGCTATGATTATGATTGGCGTACATTTGACCATGGAGAAAAGTTTGCTTTAACAAGCAAAAGACAGGATGCTTCGAGATTGGCAAAATTCTTCAACAGGTTCAAAGGGCTTCCTGTTGAACTATCGGTCACTGTATTTCTCGGTCGAAACATAATCTCTTGCTCAAAACACTATTCTGGTATTTTCTATCAAAAGGAAATTCCGGAGTGGTTCACAGAACAACAATCATCTGAGACTCTGGTGTCCCCACTACCTACAAATCTTAGAAAGGATGAGAGTTTCCTGGGATTTGTTGTGTGCGCGGTTTTTTCAGTTGAGCCGTCTCGAGCTGACGTCCTCAAATATTTGTATTCAGGACACCATCCCAGACTTGATTGCAATTTTCATTTGAGCCCTGAAATACCGTGTAGCTTAGAGGTTGGCAGCTTGCCTGGGACCAGTCCCAAGAACAAATTCATGTGGTTATATCTTAGACGGTTTGTCTGGCTGCAGTACATACCGGGTCATTATTTTAAGGATCACTTGAATGAACTCCCTGAGTTTGGGGTTTCATTTACGAGTGATTCCCCAGCCTTGGCTGTAAAGCAATACGGATTCCGTATCCTATACGACCGGCCAAGAGGTGCAAGAATACTGGAAAGCAACAGTGCAGTGCTTCACTTCCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

391

Amino Acids

45.32

Weight (kDa)

8.05

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 232 - 373 1.4e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 745
AatII GACGTC 1 cut(s) 840
AccII CGCG 3 cut(s) 92, 99, 806
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 3 cut(s) 92, 498, 806
AclWI GGATC 3 cut(s) 62, 102, 1029
AcoI YGGCCR 1 cut(s) 1118
AcsI RAATTY 5 cut(s) 260, 412, 590, 702, 956
AcuI CTGAAG 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 837
AfaI GTAC 3 cut(s) 242, 526, 998
AfiI CCNNNNNNNGG 2 cut(s) 1046, 1078
AhdI GACNNNNNGTC 1 cut(s) 983
AjnI CCWGG 3 cut(s) 425, 786, 934
AjuI GAANNNNNNNTTGG 2 cut(s) 941, 973
AluBI AGCT 6 cut(s) 7, 490, 507, 833, 915, 930
AluI AGCT 6 cut(s) 7, 490, 507, 833, 915, 930
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 3 cut(s) 104, 731, 831
Alw44I GTGCAC 1 cut(s) 334
AlwI GGATC 3 cut(s) 62, 102, 1029
Ama87I CYCGRG 1 cut(s) 828
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 1 cut(s) 1118
ApaLI GTGCAC 1 cut(s) 334
ApeKI GCWGC 3 cut(s) 469, 927, 991
ApoI RAATTY 5 cut(s) 260, 412, 590, 702, 956
Asp700I GAANNNNTTC 1 cut(s) 956
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 1 cut(s) 939
AsuC2I CCSGG 1 cut(s) 1005
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 1 cut(s) 939
BaeGI GKGCMC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 3 cut(s) 456, 939, 978
BccI CCATC 4 cut(s) 95, 328, 424, 873
BceAI ACGGC 1 cut(s) 808
BciT130I CCWGG 3 cut(s) 427, 788, 936
BciVI GTATCC 2 cut(s) 93, 1115
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 1005
BcoDI GTCTC 3 cut(s) 104, 731, 831
BfmI CTRYAG 1 cut(s) 992
BfuI GTATCC 2 cut(s) 93, 1115
BisI GCNGC 3 cut(s) 470, 928, 992
BlsI GCNGC 3 cut(s) 471, 929, 993
Bme1390I CCNGG 4 cut(s) 427, 788, 936, 1005
Bme18I GGWCC 1 cut(s) 939
BmeRI GACNNNNNGTC 1 cut(s) 983
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 1 cut(s) 939
BmiI GGNNCC 1 cut(s) 940
BmrFI CCNGG 4 cut(s) 427, 788, 936, 1005
BmsI GCATC 2 cut(s) 316, 562
BpuMI CCSGG 1 cut(s) 1005
BsaAI YACGTR 1 cut(s) 503
BsaHI GRCGYC 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 535, 787, 935, 1077
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 2 cut(s) 1046, 1078
Bse118I RCCGGY 1 cut(s) 1116
Bse1I ACTGG 2 cut(s) 942, 1143
Bse3DI GCAATG 1 cut(s) 464
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 3 cut(s) 427, 788, 936
BseDI CCNNGG 4 cut(s) 535, 787, 935, 1077
BseGI GGATG 4 cut(s) 435, 577, 781, 865
BseLI CCNNNNNNNGG 2 cut(s) 1046, 1078
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 3 cut(s) 29, 726, 1032
BseNI ACTGG 2 cut(s) 942, 1143
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 3 cut(s) 456, 939, 978
BseYI CCCAGC 1 cut(s) 1072
BsgI GTGCAG 1 cut(s) 1173
Bsh1236I CGCG 3 cut(s) 92, 99, 806
Bsh1285I CGRYCG 2 cut(s) 649, 1117
BshFI GGCC 1 cut(s) 1120
BsiEI CGRYCG 2 cut(s) 649, 1117
BsiHKAI GWGCWC 1 cut(s) 338
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 3 cut(s) 707, 1004, 1117
BslFI GGGAC 4 cut(s) 283, 733, 930, 952
BslI CCNNNNNNNGG 2 cut(s) 1046, 1078
BsmAI GTCTC 3 cut(s) 104, 731, 831
BsmBI CGTCTC 1 cut(s) 831
BsmFI GGGAC 4 cut(s) 283, 733, 930, 952
BsnI GGCC 1 cut(s) 1120
BsoBI CYCGRG 1 cut(s) 828
Bsp1286I GDGCHC 2 cut(s) 338, 899
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 5 cut(s) 67, 94, 142, 340, 1021
Bsp19I CCATGG 1 cut(s) 535
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 3 cut(s) 92, 498, 806
BspANI GGCC 1 cut(s) 1120
BspCNI CTCAG 3 cut(s) 28, 727, 1033
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 3 cut(s) 92, 99, 806
BspHI TCATGA 3 cut(s) 64, 292, 439
BspLI GGNNCC 1 cut(s) 940
BspMAI CTGCAG 1 cut(s) 996
BspPI GGATC 3 cut(s) 62, 102, 1029
BsrDI GCAATG 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 1116
BsrI ACTGG 2 cut(s) 942, 1143
BssAI RCCGGY 1 cut(s) 1116
BssECI CCNNGG 4 cut(s) 535, 787, 935, 1077
BssMI GATC 5 cut(s) 67, 94, 142, 340, 1021
BssNI GRCGYC 1 cut(s) 837
BssT1I CCWWGG 2 cut(s) 535, 1077
Bst2UI CCWGG 3 cut(s) 427, 788, 936
Bst4CI ACNGT 4 cut(s) 637, 909, 981, 1151
BstACI GRCGYC 1 cut(s) 837
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 932
BstDEI CTNAG 6 cut(s) 15, 735, 767, 916, 974, 1041
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 4 cut(s) 435, 577, 781, 865
BstFNI CGCG 3 cut(s) 92, 99, 806
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 5 cut(s) 70, 97, 145, 343, 1024
BstMAI GTCTC 3 cut(s) 104, 731, 831
BstMBI GATC 5 cut(s) 67, 94, 142, 340, 1021
BstMCI CGRYCG 2 cut(s) 649, 1117
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 3 cut(s) 427, 788, 936
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 4 cut(s) 425, 786, 934, 1003
BstSFI CTRYAG 1 cut(s) 992
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 3 cut(s) 92, 99, 806
BstV1I GCAGC 3 cut(s) 456, 939, 978
BsuI GTATCC 2 cut(s) 93, 1115
BsuRI GGCC 1 cut(s) 1120
BtgI CCRYGG 1 cut(s) 535
BtgZI GCGATG 1 cut(s) 114
BtsCI GGATG 4 cut(s) 435, 577, 781, 865
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 4 cut(s) 336, 633, 1156, 1161
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 932
CciI TCATGA 3 cut(s) 64, 292, 439
CfoI GCGC 1 cut(s) 806
Cfr10I RCCGGY 1 cut(s) 1116
Cfr13I GGNCC 1 cut(s) 939
Csp6I GTAC 3 cut(s) 241, 525, 997
CviAII CATG 6 cut(s) 65, 222, 293, 440, 536, 961
CviQI GTAC 3 cut(s) 241, 525, 997
DdeI CTNAG 6 cut(s) 15, 735, 767, 916, 974, 1041
DpnI GATC 5 cut(s) 69, 96, 144, 342, 1023
DpnII GATC 5 cut(s) 67, 94, 142, 340, 1021
DrdI GACNNNNNNGTC 1 cut(s) 745
DriI GACNNNNNGTC 1 cut(s) 983
DseDI GACNNNNNNGTC 1 cut(s) 745
EaeI YGGCCR 1 cut(s) 1118
Eam1105I GACNNNNNGTC 1 cut(s) 983
Eco130I CCWWGG 2 cut(s) 535, 1077
Eco24I GRGCYC 1 cut(s) 899
Eco47I GGWCC 1 cut(s) 939
Eco57I CTGAAG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 828
EcoRII CCWGG 3 cut(s) 425, 786, 934
EcoT14I CCWWGG 2 cut(s) 535, 1077
EcoT38I GRGCYC 1 cut(s) 899
ErhI CCWWGG 2 cut(s) 535, 1077
Esp3I CGTCTC 1 cut(s) 831
FaeI CATG 6 cut(s) 68, 225, 296, 443, 539, 964
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FaqI GGGAC 4 cut(s) 283, 733, 930, 952
FatI CATG 6 cut(s) 64, 221, 292, 439, 535, 960
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 3 cut(s) 470, 928, 992
FokI GGATG 4 cut(s) 442, 584, 788, 852
FriOI GRGCYC 1 cut(s) 899
Fsp4HI GCNGC 3 cut(s) 470, 928, 992
GlaI GCGC 1 cut(s) 805
GluI GCNGC 3 cut(s) 470, 928, 992
GsaI CCCAGC 1 cut(s) 1076
HaeIII GGCC 1 cut(s) 1120
HapII CCGG 3 cut(s) 707, 1004, 1117
HhaI GCGC 1 cut(s) 806
Hin1I GRCGYC 1 cut(s) 837
Hin1II CATG 6 cut(s) 68, 225, 296, 443, 539, 964
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HinfI GANTC 6 cut(s) 271, 289, 494, 739, 1067, 1098
HpaII CCGG 3 cut(s) 707, 1004, 1117
Hpy166II GTNNAC 2 cut(s) 336, 717
Hpy188I TCNGA 3 cut(s) 370, 410, 736
Hpy8I GTNNAC 2 cut(s) 336, 717
HpyAV CCTTC 1 cut(s) 428
HpyCH4III ACNGT 4 cut(s) 637, 909, 981, 1151
HpyCH4IV ACGT 2 cut(s) 502, 837
HpyCH4V TGCA 5 cut(s) 336, 882, 994, 1130, 1154
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 6 cut(s) 15, 735, 767, 916, 974, 1041
HpySE526I ACGT 2 cut(s) 502, 837
Hsp92I GRCGYC 1 cut(s) 837
Hsp92II CATG 6 cut(s) 68, 225, 296, 443, 539, 964
HspAI GCGC 1 cut(s) 804
Kpn2I TCCGGA 1 cut(s) 706
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 5 cut(s) 67, 94, 142, 340, 1021
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 3 cut(s) 456, 939, 978
LweI GCATC 2 cut(s) 316, 562
MaeII ACGT 2 cut(s) 502, 837
MaeIII GTNAC 1 cut(s) 631
MalI GATC 5 cut(s) 69, 96, 144, 342, 1023
MboI GATC 5 cut(s) 67, 94, 142, 340, 1021
MboII GAAGA 2 cut(s) 488, 586
MhlI GDGCHC 2 cut(s) 338, 899
MluCI AATT 8 cut(s) 32, 260, 390, 412, 590, 702, 883, 956
MlyI GAGTC 2 cut(s) 265, 733
MnlI CCTC 8 cut(s) 81, 172, 205, 225, 454, 851, 913, 1118
MroI TCCGGA 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 3 cut(s) 416, 554, 1017
MslI CAYNNNNRTG 1 cut(s) 1029
MspI CCGG 3 cut(s) 707, 1004, 1117
MspR9I CCNGG 4 cut(s) 427, 788, 936, 1005
MvaI CCWGG 3 cut(s) 427, 788, 936
MvnI CGCG 3 cut(s) 92, 99, 806
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 1 cut(s) 1005
NcoI CCATGG 1 cut(s) 535
NdeII GATC 5 cut(s) 67, 94, 142, 340, 1021
NlaIII CATG 6 cut(s) 68, 225, 296, 443, 539, 964
NlaIV GGNNCC 1 cut(s) 940
NmeAIII GCCGAG 1 cut(s) 224
NmuCI GTSAC 1 cut(s) 631
NruI TCGCGA 1 cut(s) 99
NspI RCATGY 1 cut(s) 225
PaeR7I CTCGAG 1 cut(s) 828
PagI TCATGA 3 cut(s) 64, 292, 439
PcsI WCGNNNNNNNCGW 3 cut(s) 96, 103, 273
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 4 cut(s) 289, 494, 1067, 1098
PkrI GCNGC 3 cut(s) 471, 929, 993
PleI GAGTC 2 cut(s) 265, 733
PpsI GAGTC 2 cut(s) 265, 733
Ppu21I YACGTR 1 cut(s) 503
Psp6I CCWGG 3 cut(s) 425, 786, 934
PspFI CCCAGC 1 cut(s) 1072
PspGI CCWGG 3 cut(s) 425, 786, 934
PspN4I GGNNCC 1 cut(s) 940
PspPI GGNCC 1 cut(s) 939
PstI CTGCAG 1 cut(s) 996
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 3 cut(s) 242, 526, 998
RsaNI GTAC 3 cut(s) 241, 525, 997
RseI CAYNNNNRTG 1 cut(s) 1029
SaqAI TTAA 3 cut(s) 416, 554, 1017
SatI GCNGC 3 cut(s) 470, 928, 992
Sau3AI GATC 5 cut(s) 67, 94, 142, 340, 1021
Sau96I GGNCC 1 cut(s) 939
SchI GAGTC 2 cut(s) 265, 733
ScrFI CCNGG 4 cut(s) 427, 788, 936, 1005
SduI GDGCHC 2 cut(s) 338, 899
SfaNI GCATC 2 cut(s) 316, 562
SfcI CTRYAG 1 cut(s) 992
Sfr274I CTCGAG 1 cut(s) 828
SinI GGWCC 1 cut(s) 939
SlaI CTCGAG 1 cut(s) 828
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 1 cut(s) 828
SmoI CTYRAG 1 cut(s) 828
Sse9I AATT 8 cut(s) 32, 260, 390, 412, 590, 702, 883, 956
SsiI CCGC 3 cut(s) 92, 498, 806
SspI AATATT 2 cut(s) 483, 848
StyD4I CCNGG 4 cut(s) 425, 786, 934, 1003
StyI CCWWGG 2 cut(s) 535, 1077
TaaI ACNGT 4 cut(s) 637, 909, 981, 1151
TaiI ACGT 2 cut(s) 505, 840
TaqI TCGA 5 cut(s) 177, 326, 578, 649, 829
TaqII GACCGA 2 cut(s) 619, 635
TasI AATT 8 cut(s) 32, 260, 390, 412, 590, 702, 883, 956
TatI WGTACW 2 cut(s) 240, 996
TfiI GAWTC 4 cut(s) 289, 494, 1067, 1098
Tru1I TTAA 3 cut(s) 416, 554, 1017
Tru9I TTAA 3 cut(s) 416, 554, 1017
TscAI CASTG 4 cut(s) 343, 640, 1156, 1161
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 3 cut(s) 469, 927, 991
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 8 cut(s) 53, 163, 309, 456, 878, 949, 1044, 1047
TspGWI ACGGA 2 cut(s) 1091, 1110
TspRI CASTG 4 cut(s) 343, 640, 1156, 1161
VneI GTGCAC 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 939
XapI RAATTY 5 cut(s) 260, 412, 590, 702, 956
XceI RCATGY 1 cut(s) 225
XhoI CTCGAG 1 cut(s) 828
XmnI GAANNNNTTC 1 cut(s) 956
ZraI GACGTC 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.