Rh4AG293900

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001545
Physical Location & Seq
Forward (+)
60751559 .. 60753029
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG293900.1

Sequence Viewer

Length: 1383 bp
ATGCTTAAACAAATAGTCACAAGCTTTTTGAAGGGTTTTCAACAACAGGACTTTGCTGATCCCTCCGCGAACATACTATTGTATAAGAATCAGCTCCAGGACTTTGACCGTTGCTCAATCTATAATTCTTGTTTCCCCCCAAACGAGATTGTAGAATGGTTCGAGCTTCAAAGCAATGGACCCTCAATAACAATCCTGCTGCCACCAGATCTATATAGAGATGCTAATTGGATAGGATTGGCATTCTGTGCATCCTTTGCAGCTCTGAAGAATTCAAGTGCCAACTTTGCCAATAATGTGGATCCAGGACTTCCTCACCACCTTACATGTCACTTGGAAAAGGATGATGGAGGTAGTCTGGAATCTCTCCATGACTATTGCACCACCAATGAAGAATTTCAATGGATGCATCTAGGAGGATTTATGTGGCTCTCCTATATACCGCGAACATGGTTTTCGGATCAGCTGAATGAATGTGGTTGCCTAGAGGCTTCAATTGTAAGTGGTCATGGAGCATTTAGTGTGGATAAGTGTGGGTTCCGGTTTGTATATCAGCAAGATGAAGAAGAGCTTAAGCAGACCTTAATGCTTTGCAAGACCGCTTTGTTGGATCTTTATGAGGAGAAGCAAGACCATGAGGCAGATGGAACTAGCAGAACATTGGCCGATAATCATGATGGAGAAACCAGTAATGCAAATGGTATCAGGGATCGGGACTTTGACTGTGCCTTGACGTACAATTCTTGTTTCCCTCCAAGTGATATTGTAGAGTGGTTCGGGCATCATAGCAATGGCCCTTCAGTAAAAATCCCGCTGCCATCAAATCTATACAAGGAGAATACTAATTGGGTAGGACTGGCTCTGTGCGCATACTTTTCAGTTCTTGACCCTTCGAATGCCAGCCTTGATCATTTGGATTCTGAAAAACTTGATCACCACCTTACATGTTATTTGGAAACTGATAGCGGTAGTACGGACTATTTCCATTGCTACATCACCAAAAACAAAGAATTCAAATGGTTGCATCTAGGAGGATTCTTATGGGTATCCTACATACCACGAAGGTTATTGAGTTTGGATCATCAGTCAAATGAGTGCATTAACCTGGAGGCTTCTATTGGAAGTGATCATCAAGGCTTGGGGGTGCTCAAGTGTGGGCTTCGTCTTCTATATCAACATGATGTGGAAGAGTTCAAGAAAACCATTATGCACTGCAAGACCTCCTTGTCTGAACATTACAATGGAGAAAATAAGCCTGACGTTTGGGGTGAGACCGAATCATCTAGTACTAGCAGCAAGTGTGTCATTGGGGGACCTCACAGTGGTAAATTAGAAAGACCCCAAATTTATAAGGGTAAAGGAGTGCTGGTGTCTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

460

Amino Acids

51.96

Weight (kDa)

5.08

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 45 - 189 3.6e-10 C-JID domain
C-JID PF20160 251 - 396 8.4e-11 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1350
AasI GACNNNNNNGTC 1 cut(s) 1225
Acc16I TGCGCA 1 cut(s) 868
AccII CGCG 2 cut(s) 68, 445
AciI CCGC 5 cut(s) 66, 443, 600, 812, 966
AclWI GGATC 7 cut(s) 53, 296, 309, 468, 618, 717, 1086
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 4 cut(s) 271, 395, 1010, 1344
AcuI CTGAAG 2 cut(s) 287, 783
AfaI GTAC 3 cut(s) 737, 973, 1288
AfiI CCNNNNNNNGG 1 cut(s) 1322
AflII CTTAAG 1 cut(s) 572
AflIII ACRYGT 2 cut(s) 326, 944
AgsI TTSAA 8 cut(s) 31, 41, 170, 276, 401, 495, 1015, 1195
AjnI CCWGG 3 cut(s) 96, 304, 1104
AluBI AGCT 6 cut(s) 24, 94, 166, 263, 466, 571
AluI AGCT 6 cut(s) 24, 94, 166, 263, 466, 571
Alw21I GWGCWC 1 cut(s) 1149
Alw26I GTCTC 1 cut(s) 1265
AlwI GGATC 7 cut(s) 53, 296, 309, 468, 618, 717, 1086
AoxI GGCC 2 cut(s) 663, 793
ApeKI GCWGC 4 cut(s) 199, 260, 814, 1293
ApoI RAATTY 4 cut(s) 271, 395, 1010, 1344
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 3 cut(s) 179, 794, 1313
AsuHPI GGTGA 4 cut(s) 308, 926, 988, 1280
AsuII TTCGAA 1 cut(s) 893
AvaII GGWCC 2 cut(s) 179, 1313
BamHI GGATCC 1 cut(s) 301
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 1 cut(s) 1149
BbvI GCAGC 4 cut(s) 186, 272, 801, 1305
BccI CCATC 4 cut(s) 341, 638, 671, 826
BciT130I CCWGG 3 cut(s) 98, 306, 1106
BciVI GTATCC 1 cut(s) 1057
BclI TGATCA 3 cut(s) 907, 931, 1126
BcoDI GTCTC 1 cut(s) 1265
BfaI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
BfrI CTTAAG 1 cut(s) 572
BfuI GTATCC 1 cut(s) 1057
BglII AGATCT 1 cut(s) 208
BisI GCNGC 4 cut(s) 200, 261, 815, 1294
BlsI GCNGC 4 cut(s) 201, 262, 816, 1295
BmcAI AGTACT 1 cut(s) 1288
Bme1390I CCNGG 3 cut(s) 98, 306, 1106
Bme18I GGWCC 2 cut(s) 179, 1313
BmgT120I GGNCC 3 cut(s) 179, 794, 1313
BmiI GGNNCC 4 cut(s) 181, 303, 539, 1314
BmrFI CCNGG 3 cut(s) 98, 306, 1106
BmsI GCATC 6 cut(s) 211, 260, 396, 418, 790, 1033
BpiI GAAGAC 1 cut(s) 1157
BpmI CTGGAG 2 cut(s) 80, 1127
Bpu14I TTCGAA 1 cut(s) 893
BpuEI CTTGAG 1 cut(s) 1133
BsaI GGTCTC 1 cut(s) 1265
BsaWI WCCGGW 1 cut(s) 540
BsaXI ACNNNNNCTCC 2 cut(s) 1353, 1383
Bsc4I CCNNNNNNNGG 1 cut(s) 1322
Bse1I ACTGG 2 cut(s) 687, 861
Bse3DI GCAATG 3 cut(s) 181, 796, 985
BseBI CCWGG 3 cut(s) 98, 306, 1106
BseGI GGATG 3 cut(s) 251, 349, 411
BseLI CCNNNNNNNGG 1 cut(s) 1322
BseMI GCAATG 3 cut(s) 181, 796, 985
BseNI ACTGG 2 cut(s) 687, 861
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 4 cut(s) 186, 272, 801, 1305
Bsh1236I CGCG 2 cut(s) 68, 445
BshFI GGCC 2 cut(s) 665, 795
BsiHKAI GWGCWC 1 cut(s) 1149
BsiSI CCGG 1 cut(s) 541
BslFI GGGAC 2 cut(s) 728, 1326
BslI CCNNNNNNNGG 1 cut(s) 1322
BsmAI GTCTC 1 cut(s) 1265
BsmFI GGGAC 2 cut(s) 728, 1326
BsmI GAATGC 2 cut(s) 242, 901
BsnI GGCC 2 cut(s) 665, 795
Bso31I GGTCTC 1 cut(s) 1265
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 1149
BspACI CCGC 5 cut(s) 66, 443, 600, 812, 966
BspANI GGCC 2 cut(s) 665, 795
BspFNI CGCG 2 cut(s) 68, 445
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 4 cut(s) 181, 303, 539, 1314
BspPI GGATC 7 cut(s) 53, 296, 309, 468, 618, 717, 1086
BspQI GCTCTTC 1 cut(s) 561
BspT104I TTCGAA 1 cut(s) 893
BspTI CTTAAG 1 cut(s) 572
BspTNI GGTCTC 1 cut(s) 1265
BsrDI GCAATG 3 cut(s) 181, 796, 985
BsrI ACTGG 2 cut(s) 687, 861
Bst2UI CCWGG 3 cut(s) 98, 306, 1106
Bst4CI ACNGT 3 cut(s) 110, 725, 1322
Bst6I CTCTTC 2 cut(s) 561, 1182
BstAFI CTTAAG 1 cut(s) 572
BstAPI GCANNNNNTGC 2 cut(s) 248, 257
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 1 cut(s) 901
BstF5I GGATG 3 cut(s) 251, 349, 411
BstFNI CGCG 2 cut(s) 68, 445
BstHHI GCGC 1 cut(s) 869
BstMAI GTCTC 1 cut(s) 1265
BstMWI GCNNNNNNNGC 4 cut(s) 248, 257, 287, 866
BstNI CCWGG 3 cut(s) 98, 306, 1106
BstNSI RCATGY 2 cut(s) 330, 948
BstSCI CCNGG 3 cut(s) 96, 304, 1104
BstUI CGCG 2 cut(s) 68, 445
BstV1I GCAGC 4 cut(s) 186, 272, 801, 1305
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 3 cut(s) 208, 301, 610
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 3 cut(s) 208, 301, 610
BsuI GTATCC 1 cut(s) 1057
BsuRI GGCC 2 cut(s) 665, 795
BtsCI GGATG 3 cut(s) 251, 349, 411
BtsI GCAGTG 1 cut(s) 1210
BtsIMutI CAGTG 2 cut(s) 1210, 1327
Cac8I GCNNGC 1 cut(s) 901
CciI TCATGA 1 cut(s) 673
CfoI GCGC 1 cut(s) 869
Cfr13I GGNCC 3 cut(s) 179, 794, 1313
Csp6I GTAC 3 cut(s) 736, 972, 1287
CviAII CATG 8 cut(s) 327, 371, 450, 509, 635, 674, 945, 1178
CviQI GTAC 3 cut(s) 736, 972, 1287
DrdI GACNNNNNNGTC 1 cut(s) 1225
DseDI GACNNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 561, 1182
EarI CTCTTC 2 cut(s) 561, 1182
Eco31I GGTCTC 1 cut(s) 1265
Eco47I GGWCC 2 cut(s) 179, 1313
Eco57I CTGAAG 2 cut(s) 287, 783
EcoO109I RGGNCCY 1 cut(s) 1313
EcoRI GAATTC 2 cut(s) 271, 1010
EcoRII CCWGG 3 cut(s) 96, 304, 1104
EcoT22I ATGCAT 1 cut(s) 411
FaeI CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
FalI AAGNNNNNCTT 6 cut(s) 555, 587, 566, 598, 1208, 1240
FaqI GGGAC 2 cut(s) 728, 1326
FatI CATG 8 cut(s) 326, 370, 449, 508, 634, 673, 944, 1177
FauI CCCGC 1 cut(s) 819
FbaI TGATCA 3 cut(s) 907, 931, 1126
Fnu4HI GCNGC 4 cut(s) 200, 261, 815, 1294
FokI GGATG 3 cut(s) 238, 356, 418
Fsp4HI GCNGC 4 cut(s) 200, 261, 815, 1294
FspAI RTGCGCAY 1 cut(s) 868
FspBI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
FspI TGCGCA 1 cut(s) 868
GlaI GCGC 1 cut(s) 868
GluI GCNGC 4 cut(s) 200, 261, 815, 1294
GsuI CTGGAG 2 cut(s) 80, 1127
HaeIII GGCC 2 cut(s) 665, 795
HapII CCGG 1 cut(s) 541
HhaI GCGC 1 cut(s) 869
Hin1II CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 5 cut(s) 88, 362, 917, 1035, 1277
HpaII CCGG 1 cut(s) 541
HphI GGTGA 4 cut(s) 308, 926, 988, 1280
Hpy188I TCNGA 4 cut(s) 267, 460, 922, 1231
Hpy188III TCNNGA 5 cut(s) 359, 674, 713, 884, 1195
HpyAV CCTTC 4 cut(s) 25, 807, 900, 1056
HpyCH4III ACNGT 3 cut(s) 110, 725, 1322
HpyCH4IV ACGT 2 cut(s) 734, 1260
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 257, 287, 866
HpySE526I ACGT 2 cut(s) 734, 1260
Hsp92II CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
HspAI GCGC 1 cut(s) 867
Ksp22I TGATCA 3 cut(s) 907, 931, 1126
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 99, 512
Lsp1109I GCAGC 4 cut(s) 186, 272, 801, 1305
LweI GCATC 6 cut(s) 211, 260, 396, 418, 790, 1033
MaeI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
MaeII ACGT 2 cut(s) 734, 1260
MaeIII GTNAC 2 cut(s) 16, 329
MboII GAAGA 6 cut(s) 280, 404, 575, 578, 1157, 1199
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 3 cut(s) 208, 301, 610
MhlI GDGCHC 1 cut(s) 1149
MmeI TCCRAC 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 4 cut(s) 6, 573, 584, 1101
MslI CAYNNNNRTG 2 cut(s) 789, 1239
MspA1I CMGCKG 2 cut(s) 466, 814
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 1 cut(s) 541
MspR9I CCNGG 3 cut(s) 98, 306, 1106
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 2 cut(s) 242, 901
MvaI CCWGG 3 cut(s) 98, 306, 1106
MvnI CGCG 2 cut(s) 68, 445
MwoI GCNNNNNNNGC 4 cut(s) 248, 257, 287, 866
NlaIII CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
NlaIV GGNNCC 4 cut(s) 181, 303, 539, 1314
NmuCI GTSAC 2 cut(s) 16, 329
NsbI TGCGCA 1 cut(s) 868
NsiI ATGCAT 1 cut(s) 411
NspI RCATGY 2 cut(s) 330, 948
NspV TTCGAA 1 cut(s) 893
PagI TCATGA 1 cut(s) 673
PciI ACATGT 2 cut(s) 326, 944
PciSI GCTCTTC 1 cut(s) 561
PctI GAATGC 2 cut(s) 242, 901
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 5 cut(s) 88, 362, 917, 1035, 1277
PfoI TCCNGGA 2 cut(s) 96, 304
PkrI GCNGC 4 cut(s) 201, 262, 816, 1295
PpuMI RGGWCCY 1 cut(s) 1313
PscI ACATGT 2 cut(s) 326, 944
PsiI TTATAA 1 cut(s) 1350
Psp5II RGGWCCY 1 cut(s) 1313
Psp6I CCWGG 3 cut(s) 96, 304, 1104
PspGI CCWGG 3 cut(s) 96, 304, 1104
PspN4I GGNNCC 4 cut(s) 181, 303, 539, 1314
PspPI GGNCC 3 cut(s) 179, 794, 1313
PspPPI RGGWCCY 1 cut(s) 1313
PsuI RGATCY 3 cut(s) 208, 301, 610
PvuII CAGCTG 1 cut(s) 466
RsaI GTAC 3 cut(s) 737, 973, 1288
RsaNI GTAC 3 cut(s) 736, 972, 1287
RseI CAYNNNNRTG 2 cut(s) 789, 1239
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 4 cut(s) 6, 573, 584, 1101
SatI GCNGC 4 cut(s) 200, 261, 815, 1294
Sau96I GGNCC 3 cut(s) 179, 794, 1313
ScaI AGTACT 1 cut(s) 1288
ScrFI CCNGG 3 cut(s) 98, 306, 1106
SduI GDGCHC 1 cut(s) 1149
SfaNI GCATC 6 cut(s) 211, 260, 396, 418, 790, 1033
SfuI TTCGAA 1 cut(s) 893
SinI GGWCC 2 cut(s) 179, 1313
SmiMI CAYNNNNRTG 2 cut(s) 789, 1239
SmlI CTYRAG 2 cut(s) 572, 1148
SmoI CTYRAG 2 cut(s) 572, 1148
SsiI CCGC 5 cut(s) 66, 443, 600, 812, 966
SspMI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
StyD4I CCNGG 3 cut(s) 96, 304, 1104
TaaI ACNGT 3 cut(s) 110, 725, 1322
TaiI ACGT 2 cut(s) 737, 1263
TaqI TCGA 2 cut(s) 162, 893
TaqII GACCGA 1 cut(s) 1289
TatI WGTACW 1 cut(s) 1286
TfiI GAWTC 5 cut(s) 88, 362, 917, 1035, 1277
Tru1I TTAA 4 cut(s) 6, 573, 584, 1101
Tru9I TTAA 4 cut(s) 6, 573, 584, 1101
TscAI CASTG 2 cut(s) 1217, 1327
TseFI GTSAC 2 cut(s) 16, 329
TseI GCWGC 4 cut(s) 199, 260, 814, 1293
Tsp45I GTSAC 2 cut(s) 16, 329
TspDTI ATGAA 3 cut(s) 405, 486, 576
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 2 cut(s) 1217, 1327
Vha464I CTTAAG 1 cut(s) 572
VpaK11BI GGWCC 2 cut(s) 179, 1313
XapI RAATTY 4 cut(s) 271, 395, 1010, 1344
XceI RCATGY 2 cut(s) 330, 948
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
ZrmI AGTACT 1 cut(s) 1288
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.