pycom13g10720

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001545
Physical Location & Seq
Reverse (-)
7220899 .. 7221423
525 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10720.1

Sequence Viewer

Length: 525 bp
ATGCCGCTGCCACTACGCCGCTGCCACTACATCTGTTTAATGGCGGTTCTCATTGGCTGGGATTGCCCTACTTGTGTGCGTACTTGGAGGAAGAGAATTTGGCTATTCCAGCTGGAATTTCGCACCACCGTCCGCCACCACCTTATATGTCATTTCTGGAACGTCCTGCCCATGCGTAATAAGGAACTGGAGTGGTTGTATGAGCTGGGAGGATTCATTTGGCTGTCCAACACACTGCGAGGGTGGTTTCAACTGGAATCCGATCAGTTGTTTAACGAGCGTAGCGTTCTGGAGGATTCAACTGGAAGTGATCGCCCAAGCTTGAGGGTACAAAAGTGTGGACTTCATCTCGTGTATCTGCGCGACGAGGAAGAGTTTAAACAGACCATAATGCTTTGGATGACATCTTTGCGTGAGGCAGGATCAACTACTGGCACAGCTATAACTGAGAGAATCGAAAGACCGAATGACACCAAAGGTATACGGCTACTAGTTCGATTAATCTGTCTGTCCTCCTATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

175

Amino Acids

20.75

Weight (kDa)

8.75

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 481
AccII CGCG 1 cut(s) 363
AciI CCGC 4 cut(s) 5, 19, 44, 133
AclWI GGATC 1 cut(s) 430
AcsI RAATTY 2 cut(s) 96, 116
AfaI GTAC 2 cut(s) 82, 330
AgsI TTSAA 2 cut(s) 251, 300
AhlI ACTAGT 1 cut(s) 490
AluBI AGCT 4 cut(s) 112, 205, 321, 440
AluI AGCT 4 cut(s) 112, 205, 321, 440
AlwI GGATC 1 cut(s) 430
ApeKI GCWGC 2 cut(s) 7, 21
ApoI RAATTY 2 cut(s) 96, 116
AseI ATTAAT 1 cut(s) 500
AspLEI GCGC 1 cut(s) 363
BaeI ACNNNNGTAYC 2 cut(s) 320, 353
BauI CACGAG 1 cut(s) 350
BbvI GCAGC 1 cut(s) 8
BceAI ACGGC 1 cut(s) 500
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 1 cut(s) 491
BisI GCNGC 4 cut(s) 5, 8, 19, 22
BlsI GCNGC 4 cut(s) 6, 9, 20, 23
BpmI CTGGAG 2 cut(s) 209, 311
BpuEI CTTGAG 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bse1I ACTGG 4 cut(s) 192, 258, 307, 436
BseGI GGATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 4 cut(s) 192, 258, 307, 436
BseXI GCAGC 1 cut(s) 8
BseYI CCCAGC 2 cut(s) 57, 205
Bsh1236I CGCG 1 cut(s) 363
Bsp143I GATC 3 cut(s) 262, 310, 422
BspACI CCGC 4 cut(s) 5, 19, 44, 133
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 1 cut(s) 363
BspPI GGATC 1 cut(s) 430
BsrI ACTGG 4 cut(s) 192, 258, 307, 436
BssMI GATC 3 cut(s) 262, 310, 422
BssNAI GTATAC 1 cut(s) 482
BssSI CACGAG 1 cut(s) 350
Bst1107I GTATAC 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 2 cut(s) 86, 366
BstDEI CTNAG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 405
BstFNI CGCG 1 cut(s) 363
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 3 cut(s) 265, 313, 425
BstMBI GATC 3 cut(s) 262, 310, 422
BstMWI GCNNNNNNNGC 2 cut(s) 63, 109
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 8
BstZ17I GTATAC 1 cut(s) 482
BtsCI GGATG 1 cut(s) 405
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 1 cut(s) 233
CfoI GCGC 1 cut(s) 363
Csp6I GTAC 2 cut(s) 81, 329
CviAII CATG 1 cut(s) 172
CviJI RGCY 8 cut(s) 57, 103, 112, 205, 223, 321, 440, 487
CviKI_1 RGCY 8 cut(s) 57, 103, 112, 205, 223, 321, 440, 487
CviQI GTAC 2 cut(s) 81, 329
DdeI CTNAG 1 cut(s) 447
DpnI GATC 3 cut(s) 264, 312, 424
DpnII GATC 3 cut(s) 262, 310, 422
DraI TTTAAA 1 cut(s) 379
Eam1104I CTCTTC 2 cut(s) 86, 366
EarI CTCTTC 2 cut(s) 86, 366
EciI GGCGGA 1 cut(s) 122
FaeI CATG 1 cut(s) 175
FaiI YATR 8 cut(s) 146, 148, 173, 201, 389, 443, 482, 519
FatI CATG 1 cut(s) 171
FblI GTMKAC 1 cut(s) 481
Fnu4HI GCNGC 4 cut(s) 5, 8, 19, 22
FokI GGATG 1 cut(s) 412
Fsp4HI GCNGC 4 cut(s) 5, 8, 19, 22
FspBI CTAG 1 cut(s) 491
GlaI GCGC 1 cut(s) 362
GluI GCNGC 4 cut(s) 5, 8, 19, 22
GsaI CCCAGC 2 cut(s) 61, 209
GsuI CTGGAG 2 cut(s) 209, 311
HhaI GCGC 1 cut(s) 363
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 4 cut(s) 213, 257, 296, 453
Hpy166II GTNNAC 2 cut(s) 341, 482
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 157, 290
Hpy8I GTNNAC 2 cut(s) 341, 482
Hpy99I CGWCG 1 cut(s) 368
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 109
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 1 cut(s) 162
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 262, 310, 422
Lsp1109I GCAGC 1 cut(s) 8
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 162
MalI GATC 3 cut(s) 264, 312, 424
MboI GATC 3 cut(s) 262, 310, 422
MboII GAAGA 2 cut(s) 103, 383
MluCI AATT 2 cut(s) 96, 116
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 8 cut(s) 81, 203, 233, 286, 318, 361, 409, 523
MseI TTAA 4 cut(s) 38, 273, 378, 500
MspA1I CMGCKG 3 cut(s) 7, 21, 112
MssI GTTTAAAC 1 cut(s) 379
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 63, 109
NdeII GATC 3 cut(s) 262, 310, 422
NlaIII CATG 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PfeI GAWTC 4 cut(s) 213, 257, 296, 453
PkrI GCNGC 4 cut(s) 6, 9, 20, 23
PmeI GTTTAAAC 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 500
PspFI CCCAGC 2 cut(s) 57, 205
PvuII CAGCTG 1 cut(s) 112
RsaI GTAC 2 cut(s) 82, 330
RsaNI GTAC 2 cut(s) 81, 329
SaqAI TTAA 4 cut(s) 38, 273, 378, 500
SatI GCNGC 4 cut(s) 5, 8, 19, 22
Sau3AI GATC 3 cut(s) 262, 310, 422
SetI ASST 7 cut(s) 114, 144, 165, 207, 323, 442, 481
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 2 cut(s) 96, 116
SsiI CCGC 4 cut(s) 5, 19, 44, 133
SspMI CTAG 1 cut(s) 491
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 456, 496
TaqII GACCGA 1 cut(s) 478
TasI AATT 2 cut(s) 96, 116
TauI GCSGC 2 cut(s) 7, 21
TfiI GAWTC 4 cut(s) 213, 257, 296, 453
Tru1I TTAA 4 cut(s) 38, 273, 378, 500
Tru9I TTAA 4 cut(s) 38, 273, 378, 500
TscAI CASTG 1 cut(s) 240
TseI GCWGC 2 cut(s) 7, 21
TspDTI ATGAA 2 cut(s) 205, 335
TspRI CASTG 1 cut(s) 240
VspI ATTAAT 1 cut(s) 500
XapI RAATTY 2 cut(s) 96, 116
XmiI GTMKAC 1 cut(s) 481
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.