Rroxscaffold_5G00371490

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001545
Physical Location & Seq
Forward (+)
53070780 .. 53072156
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371490.1

Sequence Viewer

Length: 798 bp
ATGGGTCCGCAGCATAACCTAACATTAGAGTTCCCTGGTGAAGACAACTTTATGGGGTCAGATCGAGTTCTTGTTATTCATATACCTCGAAAACATTTTCCAGAAGACCTCGGTGATCGCATTCAAGCTTCTTTTGAATCCCTTACTCCAGGTGTGGAGGTTCAAATGTCTGGGATGCGTCTAGAAATTCCGGAGTGGTTCACAGAACAACAATCATCTGAGACTCTGGTGTCCCCACTACCTATAAATCTTAGAAAGGATGAGAGTTTCCTGGGATTTGTTGTGTGCGCGGTTTTTTCAGTTGAGCCGTCTCGAGCTGACGTCCTCAAATATTTGTATTCAGGACACCATCCCAGACTTTCTTGCAATTTTCATTTGAGCCCTGAAATACCGTGTAGCTTAGAGGTTGGCAGCTTGTTTGGGACCAGTCCCAAGAACAAATTCATGTGGTTATATCTTAGACGGTTTGTCTGGCTGCAGTACATACCGGGTCATTATTTTAAGGATCACTTGAATGAACTCCCTGAGTTAGGGGTTTCATTTACGAGTGATTCCCCAGCCTTGGCTGTAAAGCAATGCGGATTCCGCATACTATATGACCAAGAGGTGCAAGAATACTGGAAAGCAACAGTGCAGTGCTTCACTTCCTTTTTGATGACAATGAGGATCATATTCGCCAATTCATTGAAGATGAGAGACATTCTTTTAGCATTACAAGATCTTTTGGGGGTTCTCGACAACAGGACTTTGATCGCTCTTTTGAAAACCATCTCCGAAGCAACATGCTTAAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

265

Amino Acids

30.32

Weight (kDa)

5.76

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 62 - 204 1.5e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
AatII GACGTC 1 cut(s) 324
AccII CGCG 1 cut(s) 290
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 4 cut(s) 8, 290, 579, 586
AclWI GGATC 2 cut(s) 513, 674
AcsI RAATTY 2 cut(s) 186, 440
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 530, 562
AgsI TTSAA 6 cut(s) 125, 137, 164, 514, 688, 763
AhdI GACNNNNNGTC 1 cut(s) 467
AjnI CCWGG 3 cut(s) 34, 148, 270
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 4 cut(s) 128, 317, 399, 414
AluI AGCT 4 cut(s) 128, 317, 399, 414
Alw26I GTCTC 3 cut(s) 215, 315, 690
AlwI GGATC 2 cut(s) 513, 674
Ama87I CYCGRG 1 cut(s) 312
Aor13HI TCCGGA 1 cut(s) 190
ApeKI GCWGC 3 cut(s) 10, 411, 475
ApoI RAATTY 2 cut(s) 186, 440
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 5, 423
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 2 cut(s) 50, 125
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 2 cut(s) 5, 423
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 2 cut(s) 48, 111
BbvI GCAGC 3 cut(s) 22, 423, 462
BccI CCATC 2 cut(s) 357, 776
BceAI ACGGC 1 cut(s) 292
BciT130I CCWGG 3 cut(s) 36, 150, 272
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 3 cut(s) 215, 315, 690
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 476
BglII AGATCT 1 cut(s) 718
BisI GCNGC 3 cut(s) 11, 412, 476
BlsI GCNGC 3 cut(s) 12, 413, 477
Bme1390I CCNGG 4 cut(s) 36, 150, 272, 489
Bme18I GGWCC 2 cut(s) 5, 423
BmeRI GACNNNNNGTC 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 312
BmgT120I GGNCC 2 cut(s) 5, 423
BmiI GGNNCC 2 cut(s) 6, 424
BmrFI CCNGG 4 cut(s) 36, 150, 272, 489
BmsI GCATC 1 cut(s) 165
BpiI GAAGAC 2 cut(s) 48, 111
BpmI CTGGAG 1 cut(s) 132
BpuMI CCSGG 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 34, 109, 271, 561
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 2 cut(s) 530, 562
Bse1I ACTGG 2 cut(s) 426, 623
Bse3DI GCAATG 1 cut(s) 581
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 3 cut(s) 36, 150, 272
BseDI CCNNGG 4 cut(s) 34, 109, 271, 561
BseGI GGATG 3 cut(s) 180, 265, 349
BseLI CCNNNNNNNGG 2 cut(s) 530, 562
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 2 cut(s) 210, 516
BseNI ACTGG 2 cut(s) 426, 623
BseXI GCAGC 3 cut(s) 22, 423, 462
BseYI CCCAGC 1 cut(s) 556
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 2 cut(s) 191, 488
BslFI GGGAC 3 cut(s) 217, 414, 436
BslI CCNNNNNNNGG 2 cut(s) 530, 562
BsmAI GTCTC 3 cut(s) 215, 315, 690
BsmBI CGTCTC 1 cut(s) 315
BsmFI GGGAC 3 cut(s) 217, 414, 436
BsmI GAATGC 1 cut(s) 120
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 383
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 6 cut(s) 61, 115, 505, 666, 718, 750
BspACI CCGC 4 cut(s) 8, 290, 579, 586
BspCNI CTCAG 2 cut(s) 211, 517
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 2 cut(s) 6, 424
BspMAI CTGCAG 1 cut(s) 480
BspPI GGATC 2 cut(s) 513, 674
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 2 cut(s) 426, 623
BssECI CCNNGG 4 cut(s) 34, 109, 271, 561
BssMI GATC 6 cut(s) 61, 115, 505, 666, 718, 750
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 561
Bst2UI CCWGG 3 cut(s) 36, 150, 272
Bst4CI ACNGT 3 cut(s) 393, 465, 631
BstACI GRCGYC 1 cut(s) 321
BstDEI CTNAG 5 cut(s) 219, 251, 400, 458, 525
BstENI CCTNNNNNAGG 1 cut(s) 528
BstF5I GGATG 3 cut(s) 180, 265, 349
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 6 cut(s) 64, 118, 508, 669, 721, 753
BstMAI GTCTC 3 cut(s) 215, 315, 690
BstMBI GATC 6 cut(s) 61, 115, 505, 666, 718, 750
BstMWI GCNNNNNNNGC 1 cut(s) 585
BstNI CCWGG 3 cut(s) 36, 150, 272
BstNSI RCATGY 1 cut(s) 786
BstSCI CCNGG 4 cut(s) 34, 148, 270, 487
BstSFI CTRYAG 1 cut(s) 476
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 3 cut(s) 22, 423, 462
BstV2I GAAGAC 2 cut(s) 48, 111
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BtsCI GGATG 3 cut(s) 180, 265, 349
BtsI GCAGTG 1 cut(s) 641
BtsIMutI CAGTG 2 cut(s) 636, 641
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 5, 423
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 481
CviAII CATG 2 cut(s) 445, 783
CviJI RGCY 9 cut(s) 128, 307, 317, 381, 399, 414, 475, 560, 566
CviKI_1 RGCY 9 cut(s) 128, 307, 317, 381, 399, 414, 475, 560, 566
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 5 cut(s) 219, 251, 400, 458, 525
DpnI GATC 6 cut(s) 63, 117, 507, 668, 720, 752
DpnII GATC 6 cut(s) 61, 115, 505, 666, 718, 750
DrdI GACNNNNNNGTC 1 cut(s) 229
DriI GACNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 229
Eam1105I GACNNNNNGTC 1 cut(s) 467
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 2 cut(s) 5, 423
Eco88I CYCGRG 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 528
EcoRII CCWGG 3 cut(s) 34, 148, 270
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 561
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 2 cut(s) 448, 786
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 3 cut(s) 217, 414, 436
FatI CATG 2 cut(s) 444, 782
Fnu4HI GCNGC 3 cut(s) 11, 412, 476
FokI GGATG 3 cut(s) 187, 272, 336
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 3 cut(s) 11, 412, 476
FspBI CTAG 1 cut(s) 182
GlaI GCGC 1 cut(s) 289
GluI GCNGC 3 cut(s) 11, 412, 476
GsaI CCCAGC 1 cut(s) 560
GsuI CTGGAG 1 cut(s) 132
HapII CCGG 2 cut(s) 191, 488
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 2 cut(s) 448, 786
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 4 cut(s) 137, 223, 551, 582
HpaII CCGG 2 cut(s) 191, 488
HphI GGTGA 2 cut(s) 50, 125
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 3 cut(s) 61, 220, 775
Hpy188III TCNNGA 6 cut(s) 101, 182, 191, 312, 342, 734
Hpy8I GTNNAC 1 cut(s) 201
HpyCH4III ACNGT 3 cut(s) 393, 465, 631
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 366, 478, 610, 634
HpyF10VI GCNNNNNNNGC 1 cut(s) 585
HpyF3I CTNAG 5 cut(s) 219, 251, 400, 458, 525
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 2 cut(s) 448, 786
HspAI GCGC 1 cut(s) 288
Kpn2I TCCGGA 1 cut(s) 190
Kzo9I GATC 6 cut(s) 61, 115, 505, 666, 718, 750
Lsp1109I GCAGC 3 cut(s) 22, 423, 462
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 321
MalI GATC 6 cut(s) 63, 117, 507, 668, 720, 752
MboI GATC 6 cut(s) 61, 115, 505, 666, 718, 750
MboII GAAGA 3 cut(s) 53, 116, 700
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 4 cut(s) 186, 367, 440, 679
MlyI GAGTC 1 cut(s) 217
MnlI CCTC 7 cut(s) 96, 119, 151, 335, 397, 598, 657
MroI TCCGGA 1 cut(s) 190
MroXI GAANNNNTTC 1 cut(s) 440
MseI TTAA 2 cut(s) 501, 788
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 2 cut(s) 191, 488
MspR9I CCNGG 4 cut(s) 36, 150, 272, 489
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 3 cut(s) 36, 150, 272
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 1 cut(s) 585
NciI CCSGG 1 cut(s) 489
NdeII GATC 6 cut(s) 61, 115, 505, 666, 718, 750
NlaIII CATG 2 cut(s) 448, 786
NlaIV GGNNCC 2 cut(s) 6, 424
NspI RCATGY 1 cut(s) 786
PaeR7I CTCGAG 1 cut(s) 312
PctI GAATGC 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 3 cut(s) 137, 551, 582
PkrI GCNGC 3 cut(s) 12, 413, 477
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
Psp6I CCWGG 3 cut(s) 34, 148, 270
PspFI CCCAGC 1 cut(s) 556
PspGI CCWGG 3 cut(s) 34, 148, 270
PspN4I GGNNCC 2 cut(s) 6, 424
PspPI GGNCC 2 cut(s) 5, 423
PstI CTGCAG 1 cut(s) 480
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 501, 788
SatI GCNGC 3 cut(s) 11, 412, 476
Sau3AI GATC 6 cut(s) 61, 115, 505, 666, 718, 750
Sau96I GGNCC 2 cut(s) 5, 423
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 4 cut(s) 36, 150, 272, 489
SduI GDGCHC 1 cut(s) 383
SfaNI GCATC 1 cut(s) 165
SfcI CTRYAG 1 cut(s) 476
Sfr274I CTCGAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 5, 423
SlaI CTCGAG 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
Sse9I AATT 4 cut(s) 186, 367, 440, 679
SsiI CCGC 4 cut(s) 8, 290, 579, 586
SspI AATATT 1 cut(s) 332
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 4 cut(s) 34, 148, 270, 487
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 3 cut(s) 393, 465, 631
TaiI ACGT 1 cut(s) 324
TaqI TCGA 4 cut(s) 64, 88, 313, 735
TasI AATT 4 cut(s) 186, 367, 440, 679
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 3 cut(s) 137, 551, 582
Tru1I TTAA 2 cut(s) 501, 788
Tru9I TTAA 2 cut(s) 501, 788
TscAI CASTG 2 cut(s) 636, 641
TseI GCWGC 3 cut(s) 10, 411, 475
TspDTI ATGAA 6 cut(s) 68, 362, 433, 528, 531, 672
TspRI CASTG 2 cut(s) 636, 641
VpaK11BI GGWCC 2 cut(s) 5, 423
XagI CCTNNNNNAGG 1 cut(s) 528
XapI RAATTY 2 cut(s) 186, 440
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 1 cut(s) 786
XhoI CTCGAG 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 1 cut(s) 182
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.