RchiOBHm_Chr1g0317991

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001545
Physical Location & Seq
Reverse (-)
5543429 .. 5544376
948 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54828

Sequence Viewer

Length: 948 bp
ATGCCAGGGATTGGTAAGACAACCGTTGCACAAGCTCTTTTTGTAAGAATCCGCAATCAATTTGAAGCTTTTAGTTTCATTTCTAAGGTTGGAGAAATATCTAGGAGTGAAAGTTTATTTCATATCCAAGAACAACTTTGTGATGACTTGTTGAATAGAAAAGTAAACATAAAGAATGTCAATGAAGTCATAAGCAGGAGATTGCGTGGCAAACGAGTGCTTATCGTTCTTGACAACGTGGATGAATTAGAACAAATAGAGTCTGTAGCTGGCAGTGGTGATAATTTGTATGATCGATTCGGTCCTGGTAGTAGAATCATCATAACTACAAGAGATGAAAGGTTGTTGATAAACTATGAGCCCAAAATATACAAGGTTGAGAAACTTACTGAAGATGAAGCTCTTCTGCTCTTCTGTCGGAAAGCATTTAAGAAAGACCATCCTTTGGATGGATTTGCTGAGCTGGCTTACAAATTTGTAGACTATATTGATGGTCTTCCTTTGGCTCTTGTAGTTTTGGGTTCCTCATTATTCAAAAGAAGTGTAAAAGAATGGTCTAGTAAGCTTAATAGATTGAAAGATTACAATTATTCTGGTGAGAGGAAAATCATTGATATTCTCAAAGTCAGTTTTGATGGATTAGAGAATCAAGCAGAGAAGGAAATATTTTTAGACACTGCATGTTTCTTCAAAGGCGAGGATGCATGTCGTGTAGAAAAGATATTTGAGAGTTGTGGCTACTATCCGGACATCAGTATCAATATCCTCCTTGAGAGATATTTAGTGAGTATTATAGGAGGAAAACTGTGGATGCATGATTTACTTCAAGAAATGGGCAGAGAAATTGTTCGAGGAGAGTCCCAAAAGCCAGGAAAGCGCAGCAGATTGTGGCTTCATACAGATGCCCTTCCTTTACTTAGGAATAATAAGGTGAGAAATATATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

315

Amino Acids

36.29

Weight (kDa)

8.45

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 146 5.3e-17 NB-ARC domain
NACHT PF05729 2 - 143 1.1e-10 NACHT domain
WHD_ROQ1 PF23282 216 - 287 1.1e-21 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 11, 445
AccI GTMKAC 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 745
AciI CCGC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 473
AcuI CTGAAG 1 cut(s) 411
AfiI CCNNNNNNNGG 3 cut(s) 11, 445, 449
AgsI TTSAA 6 cut(s) 65, 154, 535, 577, 691, 827
AjnI CCWGG 3 cut(s) 4, 304, 868
AluBI AGCT 6 cut(s) 35, 68, 269, 401, 463, 565
AluI AGCT 6 cut(s) 35, 68, 269, 401, 463, 565
Aor13HI TCCGGA 1 cut(s) 745
ApeKI GCWGC 1 cut(s) 879
ApoI RAATTY 1 cut(s) 473
Asp700I GAANNNNTTC 2 cut(s) 402, 846
AspLEI GCGC 1 cut(s) 879
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 3 cut(s) 290, 608, 943
AvaII GGWCC 1 cut(s) 302
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BanII GRGCYC 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 891
BccI CCATC 4 cut(s) 443, 447, 485, 629
BciT130I CCWGG 3 cut(s) 6, 306, 870
BfaI CTAG 2 cut(s) 102, 558
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 1 cut(s) 880
BlpI GCTNAGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 881
Bme1390I CCNGG 3 cut(s) 6, 306, 870
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 523
BmrFI CCNGG 3 cut(s) 6, 306, 870
BmsI GCATC 3 cut(s) 691, 801, 892
BpiI GAAGAC 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 791
Bsa29I ATCGAT 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 745
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 190, 220
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 445, 449
BseAI TCCGGA 1 cut(s) 745
BseBI CCWGG 3 cut(s) 6, 306, 870
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 5 cut(s) 247, 439, 454, 706, 816
BseLI CCNNNNNNNGG 3 cut(s) 11, 445, 449
BseMII CTCAG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 867
BseXI GCAGC 1 cut(s) 891
BshVI ATCGAT 1 cut(s) 295
BsiSI CCGG 1 cut(s) 746
BslFI GGGAC 1 cut(s) 844
BslI CCNNNNNNNGG 3 cut(s) 11, 445, 449
BsmFI GGGAC 1 cut(s) 844
Bsp1286I GDGCHC 1 cut(s) 363
Bsp13I TCCGGA 1 cut(s) 745
Bsp143I GATC 1 cut(s) 292
Bsp1720I GCTNAGC 1 cut(s) 459
BspACI CCGC 1 cut(s) 52
BspCNI CTCAG 1 cut(s) 451
BspDI ATCGAT 1 cut(s) 295
BspEI TCCGGA 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 523
BspQI GCTCTTC 2 cut(s) 408, 416
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 1 cut(s) 292
Bst2UI CCWGG 3 cut(s) 6, 306, 870
Bst4CI ACNGT 2 cut(s) 25, 807
Bst6I CTCTTC 2 cut(s) 408, 416
BstC8I GCNNGC 2 cut(s) 271, 465
BstDEI CTNAG 3 cut(s) 84, 459, 917
BstF5I GGATG 5 cut(s) 247, 439, 454, 706, 816
BstHHI GCGC 1 cut(s) 879
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 2 cut(s) 464, 874
BstNI CCWGG 3 cut(s) 6, 306, 870
BstNSI RCATGY 2 cut(s) 684, 708
BstSCI CCNGG 3 cut(s) 4, 304, 868
BstSFI CTRYAG 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 891
BstV2I GAAGAC 1 cut(s) 488
Bsu15I ATCGAT 1 cut(s) 295
BsuTUI ATCGAT 1 cut(s) 295
BtsCI GGATG 5 cut(s) 247, 439, 454, 706, 816
BtsI GCAGTG 2 cut(s) 280, 675
BtsIMutI CAGTG 2 cut(s) 280, 675
Cac8I GCNNGC 2 cut(s) 271, 465
CfoI GCGC 1 cut(s) 879
Cfr13I GGNCC 1 cut(s) 302
ClaI ATCGAT 1 cut(s) 295
CviAII CATG 3 cut(s) 681, 705, 815
DdeI CTNAG 3 cut(s) 84, 459, 917
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 408, 416
EarI CTCTTC 2 cut(s) 408, 416
Eco24I GRGCYC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 411
EcoRII CCWGG 3 cut(s) 4, 304, 868
EcoT22I ATGCAT 2 cut(s) 706, 816
EcoT38I GRGCYC 1 cut(s) 363
FaeI CATG 3 cut(s) 684, 708, 818
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 1 cut(s) 844
FatI CATG 3 cut(s) 680, 704, 814
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 1 cut(s) 880
FokI GGATG 5 cut(s) 254, 426, 461, 713, 823
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 1 cut(s) 880
FspBI CTAG 2 cut(s) 102, 558
GlaI GCGC 1 cut(s) 878
GluI GCNGC 1 cut(s) 880
HapII CCGG 1 cut(s) 746
HhaI GCGC 1 cut(s) 879
Hin1II CATG 3 cut(s) 684, 708, 818
Hin6I GCGC 1 cut(s) 877
HinP1I GCGC 1 cut(s) 877
HindIII AAGCTT 2 cut(s) 66, 563
HinfI GANTC 6 cut(s) 48, 260, 297, 315, 646, 857
HpaII CCGG 1 cut(s) 746
HphI GGTGA 3 cut(s) 290, 608, 943
Hpy166II GTNNAC 2 cut(s) 166, 481
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 3 cut(s) 230, 746, 827
Hpy8I GTNNAC 2 cut(s) 166, 481
HpyAV CCTTC 2 cut(s) 652, 917
HpyCH4III ACNGT 2 cut(s) 25, 807
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 4 cut(s) 29, 680, 704, 814
HpyF10VI GCNNNNNNNGC 2 cut(s) 464, 874
HpyF3I CTNAG 3 cut(s) 84, 459, 917
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 3 cut(s) 684, 708, 818
HspAI GCGC 1 cut(s) 877
Kpn2I TCCGGA 1 cut(s) 745
Kzo9I GATC 1 cut(s) 292
LguI GCTCTTC 2 cut(s) 408, 416
Lsp1109I GCAGC 1 cut(s) 891
LweI GCATC 3 cut(s) 691, 801, 892
MaeI CTAG 2 cut(s) 102, 558
MaeII ACGT 1 cut(s) 237
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 5 cut(s) 395, 403, 404, 488, 679
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 6 cut(s) 59, 245, 283, 473, 586, 843
MlyI GAGTC 2 cut(s) 269, 866
MmeI TCCRAC 2 cut(s) 70, 398
MnlI CCTC 6 cut(s) 535, 594, 691, 776, 791, 845
Mph1103I ATGCAT 2 cut(s) 706, 816
MroI TCCGGA 1 cut(s) 745
MroXI GAANNNNTTC 2 cut(s) 402, 846
MseI TTAA 2 cut(s) 429, 567
MslI CAYNNNNRTG 1 cut(s) 900
MspI CCGG 1 cut(s) 746
MspR9I CCNGG 3 cut(s) 6, 306, 870
MvaI CCWGG 3 cut(s) 6, 306, 870
MwoI GCNNNNNNNGC 2 cut(s) 464, 874
NdeII GATC 1 cut(s) 292
NlaIII CATG 3 cut(s) 684, 708, 818
NlaIV GGNNCC 1 cut(s) 523
NsiI ATGCAT 2 cut(s) 706, 816
NspI RCATGY 2 cut(s) 684, 708
PciSI GCTCTTC 2 cut(s) 408, 416
PdmI GAANNNNTTC 2 cut(s) 402, 846
PfeI GAWTC 4 cut(s) 48, 297, 315, 646
PflMI CCANNNNNTGG 2 cut(s) 11, 445
PkrI GCNGC 1 cut(s) 881
PleI GAGTC 2 cut(s) 268, 865
PpsI GAGTC 2 cut(s) 268, 865
Psp6I CCWGG 3 cut(s) 4, 304, 868
PspGI CCWGG 3 cut(s) 4, 304, 868
PspN4I GGNNCC 1 cut(s) 523
PspPI GGNCC 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 900
SapI GCTCTTC 2 cut(s) 408, 416
SaqAI TTAA 2 cut(s) 429, 567
SatI GCNGC 1 cut(s) 880
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 2 cut(s) 269, 866
ScrFI CCNGG 3 cut(s) 6, 306, 870
SduI GDGCHC 1 cut(s) 363
SfaNI GCATC 3 cut(s) 691, 801, 892
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 900
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 6 cut(s) 59, 245, 283, 473, 586, 843
SsiI CCGC 1 cut(s) 52
SspI AATATT 1 cut(s) 666
SspMI CTAG 2 cut(s) 102, 558
StyD4I CCNGG 3 cut(s) 4, 304, 868
TaaI ACNGT 2 cut(s) 25, 807
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 295, 850
TaqII GACCGA 1 cut(s) 290
TasI AATT 6 cut(s) 59, 245, 283, 473, 586, 843
TfiI GAWTC 4 cut(s) 48, 297, 315, 646
Tru1I TTAA 2 cut(s) 429, 567
Tru9I TTAA 2 cut(s) 429, 567
TscAI CASTG 2 cut(s) 280, 682
TseI GCWGC 1 cut(s) 879
TspDTI ATGAA 7 cut(s) 67, 110, 198, 258, 351, 411, 884
TspRI CASTG 2 cut(s) 280, 682
Van91I CCANNNNNTGG 2 cut(s) 11, 445
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 473
XceI RCATGY 2 cut(s) 684, 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 480
XmnI GAANNNNTTC 2 cut(s) 402, 846
XspI CTAG 2 cut(s) 102, 558
Zsp2I ATGCAT 2 cut(s) 706, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.