Rroxscaffold_5G00371500

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001545
Physical Location & Seq
Forward (+)
53072278 .. 53077672
5395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371500.1

Sequence Viewer

Length: 1326 bp
ATGTTTGATGTGTCTCCTTTTTTTCAGGACTTTGACCGTTGCTCAATCTATAATTCTTGTTTCCCTCCAAACGAGATTGTAGAATGGTTCGAGCTTCAAAGCAATGGACCCTCAATAACAATCCTGCTGCCACCAGATCTATATAGAGATGCTAATTGGATAGGATTGGCATTCTGTGCATCCTTTGCAGCTCTGAAGAATTCAAGTGCCAACTTTGCCAATAATGTGGATCCAGGACTTCCTCACCACCTTACATGTCACTTGGAAAAGGATGATGGAGGTAGTCTGGGATCTCTCCATGACTATTGCACCACCAATGAAGAATTTCAATGGATGCATCTAGGAGGATTTATGTGGCTCTCCTATATACCGCGAACATGGTTTTCGGATCAGCTGAGTGAATGTGGTTGCCTGGAGGCTTCAATTGTAAGTGATCATGGAGCATTTAGTGTGGATAAGTGTGGGTTCCGGCTTGTATATCAGCACGATGAAGAAGAGCTTAAGCAGACCTTAATGCTTTGCAAGACTGCTTTGTTGGATCTTTATGAGGAGAAGCAAGACCATAAGGCAGATGGAACTAGTAGAACATTGGCCGAGAATCATGCTGGAGAAACCAGTAGTGCAAATGGTATCAGGGATCGGGACTTTGACTGTGCCTTGACATACAATTCTTGTTTCCCTCCAAGTGATATTGTAGAGTGGTTCGGGCATCGTAGCAATGGCCCTTCAAAAAATCCCGCTACCATCTGTCATCGGTCAAATGAGTGCATTAACCCGGAGGCTTCTATTGGAAGTGATCATCAAGGCTTGGGGGTGCTCAAGTGTGGGCTTCGTCTTCTATATCAACATGATGTGGAAGAGTTCAAGAAAACCATTATGCACTGCAAGACCTCCTTGTCCGAACATTACAATGGAGAAAATAAGCCCGACGTTGGGGGTGAGACCGAATCATCTAGTACTAGCAGCAAGTGTGTCATTGGGGGACCTCACGGTGGTATATTAGAAAGACCCCAAATTGATAAGGGTAAAGGAGTGCTGGTGTCCGATGTGATTCCGTGTTGTTTCGGGGCGGTTGGAGTGGCTGCGGGACATGGATGGCCGGCAACGAACCGTGGGGGATATGCGGCAATTGGGGGCCGGGGGATGGTTGCCGAGTTCTCGAACCGGTCCGTTCCGGATCGGGTTTCGGGTATCAATCGGGTTGAAGAAGGCCTCGCAAAGTGTCGTGGTGATGCGGCGGTGCGGGGTTGCTGCGCGGCGGCGTTGTGCTTGCATGGGGGCGGTACGGTAACGGCGGCTGCAGATCGGCTTCGCTTTGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

441

Amino Acids

47.99

Weight (kDa)

5.35

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 21 - 165 4.8e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 895
AccII CGCG 2 cut(s) 373, 1256
AccIII TCCGGA 1 cut(s) 1174
AclWI GGATC 7 cut(s) 224, 237, 298, 396, 546, 645, 1185
AcoI YGGCCR 2 cut(s) 591, 1097
AcsI RAATTY 2 cut(s) 199, 323
AcuI CTGAAG 1 cut(s) 215
AfaI GTAC 2 cut(s) 958, 1285
AfiI CCNNNNNNNGG 4 cut(s) 932, 933, 992, 1144
AflII CTTAAG 1 cut(s) 500
AflIII ACRYGT 1 cut(s) 254
AgeI ACCGGT 1 cut(s) 1164
AgsI TTSAA 7 cut(s) 98, 204, 329, 423, 729, 865, 1205
AhlI ACTAGT 1 cut(s) 578
AjnI CCWGG 2 cut(s) 232, 411
AluBI AGCT 4 cut(s) 94, 191, 394, 499
AluI AGCT 4 cut(s) 94, 191, 394, 499
Alw21I GWGCWC 1 cut(s) 819
Alw26I GTCTC 2 cut(s) 18, 935
AlwI GGATC 7 cut(s) 224, 237, 298, 396, 546, 645, 1185
Aor13HI TCCGGA 1 cut(s) 1174
AoxI GGCC 5 cut(s) 591, 721, 1097, 1135, 1210
ApeKI GCWGC 6 cut(s) 127, 188, 963, 1082, 1251, 1298
ApoI RAATTY 2 cut(s) 199, 323
AsiGI ACCGGT 1 cut(s) 1164
Asp700I GAANNNNTTC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 1256
AspS9I GGNCC 5 cut(s) 107, 722, 983, 1135, 1167
AsuC2I CCSGG 2 cut(s) 776, 1139
AsuHPI GGTGA 3 cut(s) 236, 950, 1241
AvaII GGWCC 3 cut(s) 107, 983, 1167
BamHI GGATCC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 827
Bbv12I GWGCWC 1 cut(s) 819
BbvI GCAGC 6 cut(s) 114, 200, 975, 1069, 1238, 1285
BccI CCATC 5 cut(s) 269, 566, 752, 1089, 1138
BceAI ACGGC 1 cut(s) 1308
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 2 cut(s) 234, 413
BclI TGATCA 2 cut(s) 433, 796
BcnI CCSGG 2 cut(s) 776, 1139
BcoDI GTCTC 2 cut(s) 18, 935
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 4 cut(s) 341, 579, 954, 960
BfmI CTRYAG 1 cut(s) 1299
BfrI CTTAAG 1 cut(s) 500
BglII AGATCT 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 958
Bme1390I CCNGG 4 cut(s) 234, 413, 776, 1139
Bme18I GGWCC 3 cut(s) 107, 983, 1167
BmgT120I GGNCC 5 cut(s) 107, 722, 983, 1135, 1167
BmiI GGNNCC 5 cut(s) 109, 231, 467, 984, 1136
BmrFI CCNGG 4 cut(s) 234, 413, 776, 1139
BmsI GCATC 6 cut(s) 139, 188, 324, 346, 718, 1222
BpiI GAAGAC 1 cut(s) 827
BpmI CTGGAG 2 cut(s) 434, 627
BpuEI CTTGAG 1 cut(s) 803
BpuMI CCSGG 2 cut(s) 776, 1139
BsaI GGTCTC 1 cut(s) 935
BsaJI CCNNGG 2 cut(s) 1111, 1138
BsaWI WCCGGW 2 cut(s) 1164, 1174
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bsc4I CCNNNNNNNGG 4 cut(s) 932, 933, 992, 1144
Bse118I RCCGGY 2 cut(s) 1099, 1164
Bse1I ACTGG 1 cut(s) 615
Bse3DI GCAATG 2 cut(s) 109, 724
BseAI TCCGGA 1 cut(s) 1174
BseBI CCWGG 2 cut(s) 234, 413
BseDI CCNNGG 2 cut(s) 1111, 1138
BseGI GGATG 5 cut(s) 179, 277, 339, 1100, 1149
BseLI CCNNNNNNNGG 4 cut(s) 932, 933, 992, 1144
BseMI GCAATG 2 cut(s) 109, 724
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 1 cut(s) 615
BseRI GAGGAG 1 cut(s) 563
BseXI GCAGC 6 cut(s) 114, 200, 975, 1069, 1238, 1285
Bsh1236I CGCG 2 cut(s) 373, 1256
BshFI GGCC 5 cut(s) 593, 723, 1099, 1137, 1212
BshTI ACCGGT 1 cut(s) 1164
BsiHKAI GWGCWC 1 cut(s) 819
BsiSI CCGG 6 cut(s) 469, 776, 1100, 1138, 1165, 1175
BslFI GGGAC 3 cut(s) 656, 996, 1101
BslI CCNNNNNNNGG 4 cut(s) 932, 933, 992, 1144
BsmAI GTCTC 2 cut(s) 18, 935
BsmFI GGGAC 3 cut(s) 656, 996, 1101
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 5 cut(s) 593, 723, 1099, 1137, 1212
Bso31I GGTCTC 1 cut(s) 935
Bsp1286I GDGCHC 1 cut(s) 819
Bsp13I TCCGGA 1 cut(s) 1174
BspANI GGCC 5 cut(s) 593, 723, 1099, 1137, 1212
BspCNI CTCAG 1 cut(s) 387
BspEI TCCGGA 1 cut(s) 1174
BspFNI CGCG 2 cut(s) 373, 1256
BspLI GGNNCC 5 cut(s) 109, 231, 467, 984, 1136
BspMAI CTGCAG 1 cut(s) 1303
BspPI GGATC 7 cut(s) 224, 237, 298, 396, 546, 645, 1185
BspQI GCTCTTC 1 cut(s) 489
BspTI CTTAAG 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 935
BsrDI GCAATG 2 cut(s) 109, 724
BsrFI RCCGGY 2 cut(s) 1099, 1164
BsrI ACTGG 1 cut(s) 615
BssAI RCCGGY 2 cut(s) 1099, 1164
BssECI CCNNGG 2 cut(s) 1111, 1138
Bst2UI CCWGG 2 cut(s) 234, 413
Bst4CI ACNGT 5 cut(s) 38, 653, 992, 1112, 1288
Bst6I CTCTTC 2 cut(s) 489, 852
BstAFI CTTAAG 1 cut(s) 500
BstAPI GCANNNNNTGC 2 cut(s) 176, 185
BstC8I GCNNGC 2 cut(s) 1101, 1271
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 1 cut(s) 1111
BstF5I GGATG 5 cut(s) 179, 277, 339, 1100, 1149
BstFNI CGCG 2 cut(s) 373, 1256
BstHHI GCGC 1 cut(s) 1256
BstMAI GTCTC 2 cut(s) 18, 935
BstMWI GCNNNNNNNGC 3 cut(s) 176, 185, 215
BstNI CCWGG 2 cut(s) 234, 413
BstNSI RCATGY 1 cut(s) 258
BstSCI CCNGG 4 cut(s) 232, 411, 774, 1137
BstSFI CTRYAG 1 cut(s) 1299
BstUI CGCG 2 cut(s) 373, 1256
BstV1I GCAGC 6 cut(s) 114, 200, 975, 1069, 1238, 1285
BstV2I GAAGAC 1 cut(s) 827
BstX2I RGATCY 4 cut(s) 136, 229, 290, 538
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 4 cut(s) 136, 229, 290, 538
BsuRI GGCC 5 cut(s) 593, 723, 1099, 1137, 1212
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 5 cut(s) 179, 277, 339, 1100, 1149
BtsI GCAGTG 1 cut(s) 880
BtsIMutI CAGTG 1 cut(s) 880
Cac8I GCNNGC 2 cut(s) 1101, 1271
CfoI GCGC 1 cut(s) 1256
Cfr10I RCCGGY 2 cut(s) 1099, 1164
Cfr13I GGNCC 5 cut(s) 107, 722, 983, 1135, 1167
CpoI CGGWCCG 1 cut(s) 1167
Csp6I GTAC 2 cut(s) 957, 1284
CspAI ACCGGT 1 cut(s) 1164
CspI CGGWCCG 1 cut(s) 1167
CviAII CATG 8 cut(s) 255, 299, 378, 437, 602, 848, 1091, 1274
CviQI GTAC 2 cut(s) 957, 1284
DdeI CTNAG 1 cut(s) 395
DrdI GACNNNNNNGTC 1 cut(s) 895
DseDI GACNNNNNNGTC 1 cut(s) 895
EaeI YGGCCR 2 cut(s) 591, 1097
Eam1104I CTCTTC 2 cut(s) 489, 852
EarI CTCTTC 2 cut(s) 489, 852
Eco147I AGGCCT 1 cut(s) 1212
Eco31I GGTCTC 1 cut(s) 935
Eco47I GGWCC 3 cut(s) 107, 983, 1167
Eco57I CTGAAG 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 983
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 2 cut(s) 232, 411
EcoT22I ATGCAT 1 cut(s) 339
FaeI CATG 8 cut(s) 258, 302, 381, 440, 605, 851, 1094, 1277
FalI AAGNNNNNCTT 6 cut(s) 483, 515, 494, 526, 878, 910
FaqI GGGAC 3 cut(s) 656, 996, 1101
FatI CATG 8 cut(s) 254, 298, 377, 436, 601, 847, 1090, 1273
FauI CCCGC 3 cut(s) 745, 1078, 1236
FbaI TGATCA 2 cut(s) 433, 796
FokI GGATG 5 cut(s) 166, 284, 346, 1107, 1156
FspBI CTAG 4 cut(s) 341, 579, 954, 960
GlaI GCGC 1 cut(s) 1255
GsuI CTGGAG 2 cut(s) 434, 627
HaeIII GGCC 5 cut(s) 593, 723, 1099, 1137, 1212
HapII CCGG 6 cut(s) 469, 776, 1100, 1138, 1165, 1175
HhaI GCGC 1 cut(s) 1256
Hin1II CATG 8 cut(s) 258, 302, 381, 440, 605, 851, 1094, 1277
Hin6I GCGC 1 cut(s) 1254
HinP1I GCGC 1 cut(s) 1254
HinfI GANTC 3 cut(s) 598, 947, 1051
HpaII CCGG 6 cut(s) 469, 776, 1100, 1138, 1165, 1175
HphI GGTGA 3 cut(s) 236, 950, 1241
Hpy188I TCNGA 4 cut(s) 195, 388, 901, 1045
Hpy188III TCNNGA 5 cut(s) 26, 641, 865, 1159, 1175
Hpy99I CGWCG 1 cut(s) 932
HpyAV CCTTC 2 cut(s) 735, 1202
HpyCH4III ACNGT 5 cut(s) 38, 653, 992, 1112, 1288
HpyCH4IV ACGT 1 cut(s) 930
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 185, 215
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 1 cut(s) 930
Hsp92II CATG 8 cut(s) 258, 302, 381, 440, 605, 851, 1094, 1277
HspAI GCGC 1 cut(s) 1254
Kpn2I TCCGGA 1 cut(s) 1174
KroI GCCGGC 1 cut(s) 1099
KroNI GCCGGC 1 cut(s) 1101
Ksp22I TGATCA 2 cut(s) 433, 796
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 6 cut(s) 114, 200, 975, 1069, 1238, 1285
LweI GCATC 6 cut(s) 139, 188, 324, 346, 718, 1222
MaeI CTAG 4 cut(s) 341, 579, 954, 960
MaeII ACGT 1 cut(s) 930
MaeIII GTNAC 2 cut(s) 257, 1288
MboII GAAGA 7 cut(s) 208, 332, 503, 506, 827, 869, 1217
MfeI CAATTG 2 cut(s) 423, 1128
MflI RGATCY 4 cut(s) 136, 229, 290, 538
MhlI GDGCHC 1 cut(s) 819
MluCI AATT 8 cut(s) 52, 154, 199, 323, 423, 667, 1014, 1128
MmeI TCCRAC 2 cut(s) 516, 1054
Mph1103I ATGCAT 1 cut(s) 339
MroI TCCGGA 1 cut(s) 1174
MroNI GCCGGC 1 cut(s) 1099
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 501, 512, 771
MslI CAYNNNNRTG 1 cut(s) 909
MspA1I CMGCKG 1 cut(s) 394
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 6 cut(s) 469, 776, 1100, 1138, 1165, 1175
MspR9I CCNGG 4 cut(s) 234, 413, 776, 1139
MunI CAATTG 2 cut(s) 423, 1128
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 2 cut(s) 234, 413
MvnI CGCG 2 cut(s) 373, 1256
MwoI GCNNNNNNNGC 3 cut(s) 176, 185, 215
NaeI GCCGGC 1 cut(s) 1101
NciI CCSGG 2 cut(s) 776, 1139
NgoMIV GCCGGC 1 cut(s) 1099
NlaIII CATG 8 cut(s) 258, 302, 381, 440, 605, 851, 1094, 1277
NlaIV GGNNCC 5 cut(s) 109, 231, 467, 984, 1136
NmeAIII GCCGAG 2 cut(s) 619, 1177
NmuCI GTSAC 1 cut(s) 257
NsiI ATGCAT 1 cut(s) 339
NspI RCATGY 1 cut(s) 258
PceI AGGCCT 1 cut(s) 1212
PciI ACATGT 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 489
PctI GAATGC 1 cut(s) 170
PdiI GCCGGC 1 cut(s) 1101
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 3 cut(s) 598, 947, 1051
PfoI TCCNGGA 1 cut(s) 232
PinAI ACCGGT 1 cut(s) 1164
PpuMI RGGWCCY 1 cut(s) 983
PscI ACATGT 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 983
Psp6I CCWGG 2 cut(s) 232, 411
PspGI CCWGG 2 cut(s) 232, 411
PspN4I GGNNCC 5 cut(s) 109, 231, 467, 984, 1136
PspPI GGNCC 5 cut(s) 107, 722, 983, 1135, 1167
PspPPI RGGWCCY 1 cut(s) 983
PstI CTGCAG 1 cut(s) 1303
PsuI RGATCY 4 cut(s) 136, 229, 290, 538
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 2 cut(s) 958, 1285
RsaNI GTAC 2 cut(s) 957, 1284
RseI CAYNNNNRTG 1 cut(s) 909
Rsr2I CGGWCCG 1 cut(s) 1167
RsrII CGGWCCG 1 cut(s) 1167
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 3 cut(s) 501, 512, 771
Sau96I GGNCC 5 cut(s) 107, 722, 983, 1135, 1167
ScaI AGTACT 1 cut(s) 958
ScrFI CCNGG 4 cut(s) 234, 413, 776, 1139
SduI GDGCHC 1 cut(s) 819
SfaNI GCATC 6 cut(s) 139, 188, 324, 346, 718, 1222
SfcI CTRYAG 1 cut(s) 1299
SinI GGWCC 3 cut(s) 107, 983, 1167
SmiMI CAYNNNNRTG 1 cut(s) 909
SmlI CTYRAG 2 cut(s) 500, 818
SmoI CTYRAG 2 cut(s) 500, 818
SpeI ACTAGT 1 cut(s) 578
Sse9I AATT 8 cut(s) 52, 154, 199, 323, 423, 667, 1014, 1128
SseBI AGGCCT 1 cut(s) 1212
SspMI CTAG 4 cut(s) 341, 579, 954, 960
StuI AGGCCT 1 cut(s) 1212
StyD4I CCNGG 4 cut(s) 232, 411, 774, 1137
TaaI ACNGT 5 cut(s) 38, 653, 992, 1112, 1288
TaiI ACGT 1 cut(s) 933
TaqI TCGA 2 cut(s) 90, 1160
TaqII GACCGA 2 cut(s) 744, 959
TasI AATT 8 cut(s) 52, 154, 199, 323, 423, 667, 1014, 1128
TatI WGTACW 1 cut(s) 956
TauI GCSGC 5 cut(s) 1127, 1238, 1259, 1262, 1298
TfiI GAWTC 3 cut(s) 598, 947, 1051
Tru1I TTAA 3 cut(s) 501, 512, 771
Tru9I TTAA 3 cut(s) 501, 512, 771
TscAI CASTG 1 cut(s) 887
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 6 cut(s) 127, 188, 963, 1082, 1251, 1298
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 2 cut(s) 333, 504
TspGWI ACGGA 2 cut(s) 1044, 1159
TspRI CASTG 1 cut(s) 887
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 3 cut(s) 107, 983, 1167
XapI RAATTY 2 cut(s) 199, 323
XceI RCATGY 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 4 cut(s) 341, 579, 954, 960
ZrmI AGTACT 1 cut(s) 958
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.