Rw4G025430

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001545
Physical Location & Seq
Forward (+)
51518200 .. 51527579
9380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025430.1

Sequence Viewer

Length: 3546 bp
ATGGAGCTATTTTCTCAGGGTGGTTGTTTTCAATTTTTTTTTGGGGCTGAAACTGATATTGCTTCATGGTTCTGTCGTCAAAGTACTAATTCGTCAATAAAAATTCCATTACCTCAAGACTTGGATGGGAAAAGTCAATGGATTGGTTTTGCTCTATGGTTTCTTATTGAATCGGAGGTGCATGACAGTGTGGATTCAGAAGAAGATCCTCAATCCGAATATTTCAACAAGAGAAATTACAGTTGTGACATCTTCACAAATGATGAAGATCCTCCCCTAAAGTATCCACTCGCGGATTGCGATGGTCGTGAGACATATCAGCATTTGCTTTATATACCAGCAGATCATTTCTTGAAAAGGTTGAACAAATGCTTCATCGAGGCTAAAATCAACATCAAAAGAACAGGGGTGGAAGTGGTAGCATGTAGAGCGAGGCTATTGTACTCGGCAGATGTGCCAGAATTTGTCGGGACTCTGGTTCACCTTTGGAATCATGAACAAGTGGGGCATCTGGCAAATCCATCATCGAGCAAGTGCACTGATCAGTCAAAAGTTTATCCTTTTGCTTCAGAAACAAGTTTCTTGATTGAAATTGAACGCCAGATTTTCAGAAATTTAAGTTCAACCAGGATGGAAGGCCATGAAGTGAGAACAAGGGTAGAGGCATTGCTGCTTGAAGAAATATTTGAGCTGGAATCCGCACGTAGCTATGATTATGGTTTCATTTTCTCTCTCAGAGCAATTTTACCGTGGTTTACTCATCAAAGCATCGGCAGTGTAGTCCTTTCACTAAACGACCAAAGCATTGGGGGAGTAGTAGGCATGTGTCTTCCACCACTTTTACATAGCGATAACGAATGGGTAGGGTTTTCCCTATTTGTGGCCTTCACTCTGCCTCCTCCAGGTGTTTTGGCTGTAAAGTATTCTTCCGTTAACTGTCGTTTGCTTACCTCCAGAGGAATAATGGGTCCGCAGCATAACCTAACATTAGAGTTCCCCGGTGAAGACAACTTTATGGGGTCAGAGCGAGTTCTTGTTATTCATATACCTCGAAAACATTTTCCAGAAGACCTCGGTGATCGCATTCAAGCTTCTTTTGAATCCCTCACTCCAGGTGTGGAGGTTCAAATGTCTGGGATGCGTCTAGTATACCGGCAAGATTTGAATGGCTTAATGCAAACTATTACTCACTGCACTCTACGCAGTCCATTTGCTTACTTCGGACAAGATGACAAAACATTTATGTTGGAAGAAAATAAGATTGGTGTACATTTGACCATGGAGAAAAGTTTGCTTCAACAAGCAAAAGACAGGATGCTTCAAGATTGGCAAAATTCTTCAACAGGTTCAAAGGTAATCACTGATCAACAAATTGAGTTGATGATACTTTCTGAAACTCCAGTCAATCCACCTAAGAAAAAACACTCAGTAATCAAGTGGAAAAGAAACCTTGAGCTTTTGCTTCAATTTTTCTTTAGGCTTCCTGTTGAACTATCGGTCACTGTATTTCTCAGTCAAAACATAATCTCTTGCTCAAAACGCTATTCTGGTATTTTCTATCAAAAGGAAATTCCGGAGTGGTTCACAGAACAACAATCATCTGAGACTCTGGTGTCCCCAGTACCTACAAATCTTGGAAAGGATGAGAGTTTCCTGGGATTTGTTGTGTGCGCGGTTTTTTCAGTTGAGCCTTCTCGAGCTGACGTCCTCAAATATTTGTATTCAGGACACCATCCCAGACTTGCTTGCAATTTTCATTTGAGCCCTGAAATACCGTGTAGCTTAAAGGTTGGCAACTTGCCCGGGACCAGTCCCAAGAACAAATTCATGTGGTTATATCTTAGACGGTTTGTCTGGCTGCAGTACATACCGGGTCATTATTTTAAGGATCACTTGAATGAACTCCCTGAGTTAGGGGTTTCATTTACGAGTGATTCCCCAGCCTTGGCTGTAAAGCAATGCGGATTCCGCATCCTATATGACCAAGAGGTGCAAGAATACTGGAAAGCAATAGTGCAGTGCTTCACTTCCTTTTTTGACGACAATGAGGATCATATTCGCCAATTCATTGAAGATGAGAGACATTCTTTTAGCATTACAAGATCTTTTGGGGGTTCTCGACAACAGGACTTTGATCGCTCTTTTGAGAACCATCTCCGAAGCAACATGCTTAAACAAATAGTCACAAACTTTTTGAAGGGTTTTCAACAACAGGACTTTGATCCCTCCGCGAACATACTATTGTATAAGAATCAGCTCCAGGACTTTGACCGATGCTCAATCTATAATTCTTGTTTCCCTCCAAACGAGATTGCAGAATGGTTCGAGCTTCAAAGAAATGGACCCTCGATAACAATCCTGCTGCCACCAGATCTATCTAGAGATGCTAATTGGATAGGATTGGCATTCTGTGCATCCTTCGCAGCTCTGAAGAATTCAAGTGCCAACTTTGCCAATAATGTGGATCCAGGACTTCCTCACCACCTTACATGTCACTTGGAAAAGGATGATGGAGGTAGTCTGGAATCTCTCCATGACTATTGCACCACCAATGAAGAATTTCAATGGATGCATCTAGGAGGATTTATGTGGCTCTCCTATATACCGCGAACATGGTTTTCAGATCAGCTGAATGAATGTGGTTGCCTGGAGGCTTCAATTGTAAGTGGTCATGGAGCATTTAATGTGGATAAGTGTGGGTTCCGGCTTGTATATCAGCAAGATGAAGAAGAGCTTAAGCAGACCTTAATGCTTTGCAAGACCACTTTGTTGGATCTTTATGAGGAGAAGCAAGACCATGAGGCAGATGGAACTAGTAGAACATTGGCCGATAATCATGATGGAGAAACCAGTAGTGCAAATGGTATCAGGGATCAGGACTTTGACTGTGCCTTGACGTACAATTCTTGTTTCCCTCCAAGTGATATTGTTGATTGGTTTGGGCATCATAGCAATGGCCCTTCAGTAAAAATCCCGCTACCATCAAATCTATACAAGGAGAATACTAATTGGGTAGGACTGGCTCTGTGCTCATACTTTTCAGTTCTTGACCCTTCGAATGCCAGCCTTGATCATTTGGATTCTGAAAAACTTGATCACCACCTTACATGTTATTTGGAAACTGATAGCGGTAGTACGGACATTTTCCATTGCTACATCACCAAAAACAAAGAATTCAAATGGTTGCATCTAGGAGGATTCTTATGGGTATCCTACATACCACAAAGGTTATTGAGTTTGGGTCATCAGTCAAATGAGTGCATTAACCTGGAGGCTTCTATTGGAAGTGATCATCAAGGCTTGGGGGTGCTCAAGTGTGGGCTTCGTCTTCTATATCAACATGATGTGGAAGAGTTCAAGAAAACCATTATGCACTGCAAGACCTCCTTGTCTGAACATTACAATGGAGAAAATAAGCCTGACGTTGGGGGTGAGACCGAATCATCTAGTACTAGCAGCAAGTGTGTCATTGGGGGACCGCACAGTGGTATATTAGAAAGACCCCAAATTGATAAGGGTAAAGGAGTGCTGGTGTCTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1181

Amino Acids

134.49

Weight (kDa)

5.33

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 521 - 665 4.2e-08 C-JID domain
C-JID PF20160 766 - 910 7e-09 C-JID domain
C-JID PF20160 972 - 1117 1.4e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1612, 3388
AatII GACGTC 1 cut(s) 1707
AccI GTMKAC 1 cut(s) 1149
AccII CGCG 4 cut(s) 293, 1673, 2231, 2608
AccIII TCCGGA 1 cut(s) 1573
AclWI GGATC 9 cut(s) 200, 263, 1896, 2058, 2216, 2459, 2472, 2781, 2880
AcoI YGGCCR 1 cut(s) 2826
AcsI RAATTY 9 cut(s) 102, 461, 613, 1333, 1569, 1823, 2434, 2558, 3173
AcuI CTGAAG 3 cut(s) 552, 2450, 2946
AcyI GRCGYC 1 cut(s) 1704
AfaI GTAC 8 cut(s) 85, 443, 1269, 1623, 1865, 2900, 3136, 3451
AfiI CCNNNNNNNGG 6 cut(s) 880, 902, 1913, 1945, 3425, 3485
AflII CTTAAG 1 cut(s) 2735
AflIII ACRYGT 2 cut(s) 2489, 3107
AhdI GACNNNNNGTC 1 cut(s) 1850
AhlI ACTAGT 1 cut(s) 2813
AjnI CCWGG 8 cut(s) 626, 901, 1111, 1653, 2259, 2467, 2646, 3267
AjuI GAANNNNNNNTTGG 4 cut(s) 1245, 1277, 1808, 1840
Alw21I GWGCWC 3 cut(s) 539, 3032, 3312
Alw26I GTCTC 4 cut(s) 305, 1598, 2074, 3428
Alw44I GTGCAC 1 cut(s) 535
AlwI GGATC 9 cut(s) 200, 263, 1896, 2058, 2216, 2459, 2472, 2781, 2880
Ama87I CYCGRG 2 cut(s) 1695, 1802
Aor13HI TCCGGA 1 cut(s) 1573
AoxI GGCC 4 cut(s) 637, 882, 2826, 2956
ApaLI GTGCAC 1 cut(s) 535
ApeKI GCWGC 6 cut(s) 670, 973, 1858, 2362, 2423, 3456
ApoI RAATTY 9 cut(s) 102, 461, 613, 1333, 1569, 1823, 2434, 2558, 3173
Asp700I GAANNNNTTC 2 cut(s) 1823, 2559
AspLEI GCGC 1 cut(s) 1673
AspS9I GGNCC 5 cut(s) 968, 1806, 2342, 2957, 3476
AsuC2I CCSGG 4 cut(s) 999, 1803, 1804, 1872
AsuHPI GGTGA 7 cut(s) 473, 1013, 1088, 2471, 3089, 3151, 3443
AsuII TTCGAA 1 cut(s) 3056
AvaI CYCGRG 2 cut(s) 1695, 1802
AvaII GGWCC 4 cut(s) 968, 1806, 2342, 3476
BaeGI GKGCMC 1 cut(s) 539
BamHI GGATCC 1 cut(s) 2464
BanII GRGCYC 1 cut(s) 1766
BbsI GAAGAC 4 cut(s) 821, 1011, 1074, 3320
Bbv12I GWGCWC 3 cut(s) 539, 3032, 3312
BbvI GCAGC 6 cut(s) 657, 985, 1845, 2349, 2435, 3468
BciT130I CCWGG 8 cut(s) 628, 903, 1113, 1655, 2261, 2469, 2648, 3269
BciVI GTATCC 2 cut(s) 294, 3220
BclI TGATCA 5 cut(s) 541, 1363, 3070, 3094, 3289
BcnI CCSGG 4 cut(s) 999, 1803, 1804, 1872
BcoDI GTCTC 4 cut(s) 305, 1598, 2074, 3428
BcuI ACTAGT 1 cut(s) 2813
BfaI CTAG 7 cut(s) 1145, 2379, 2576, 2814, 3191, 3447, 3453
BfmI CTRYAG 1 cut(s) 1859
BfrI CTTAAG 1 cut(s) 2735
BfuI GTATCC 2 cut(s) 294, 3220
BglII AGATCT 2 cut(s) 2102, 2371
BisI GCNGC 6 cut(s) 671, 974, 1859, 2363, 2424, 3457
BlsI GCNGC 6 cut(s) 672, 975, 1860, 2364, 2425, 3458
BmcAI AGTACT 2 cut(s) 85, 3451
Bme18I GGWCC 4 cut(s) 968, 1806, 2342, 3476
BmeRI GACNNNNNGTC 1 cut(s) 1850
BmeT110I CYCGRG 2 cut(s) 1695, 1802
BmgT120I GGNCC 5 cut(s) 968, 1806, 2342, 2957, 3476
BmiI GGNNCC 6 cut(s) 969, 1807, 2344, 2466, 2702, 3477
BmrI ACTGGG 1 cut(s) 1613
BmuI ACTGGG 1 cut(s) 1613
BpiI GAAGAC 4 cut(s) 821, 1011, 1074, 3320
BpmI CTGGAG 7 cut(s) 885, 937, 1095, 1383, 2243, 2669, 3290
Bpu14I TTCGAA 1 cut(s) 3056
BpuEI CTTGAG 3 cut(s) 99, 1472, 3296
BpuMI CCSGG 4 cut(s) 999, 1803, 1804, 1872
BsaAI YACGTR 1 cut(s) 704
BsaBI GATNNNNATC 2 cut(s) 267, 2354
BsaHI GRCGYC 1 cut(s) 1704
BsaI GGTCTC 1 cut(s) 3428
BsaJI CCNNGG 7 cut(s) 749, 997, 1072, 1278, 1654, 1802, 1944
BsaWI WCCGGW 1 cut(s) 1573
BsaXI ACNNNNNCTCC 4 cut(s) 880, 910, 3516, 3546
Bsc4I CCNNNNNNNGG 6 cut(s) 880, 902, 1913, 1945, 3425, 3485
Bse118I RCCGGY 1 cut(s) 1152
Bse1I ACTGG 6 cut(s) 1400, 1619, 1809, 2006, 2850, 3024
Bse3DI GCAATG 4 cut(s) 665, 1964, 2959, 3148
Bse8I GATNNNNATC 2 cut(s) 267, 2354
BseAI TCCGGA 1 cut(s) 1573
BseBI CCWGG 8 cut(s) 628, 903, 1113, 1655, 2261, 2469, 2648, 3269
BseDI CCNNGG 7 cut(s) 749, 997, 1072, 1278, 1654, 1802, 1944
BseJI GATNNNNATC 2 cut(s) 267, 2354
BseLI CCNNNNNNNGG 6 cut(s) 880, 902, 1913, 1945, 3425, 3485
BseMI GCAATG 4 cut(s) 665, 1964, 2959, 3148
BseMII CTCAG 6 cut(s) 29, 748, 1440, 1525, 1593, 1899
BseNI ACTGG 6 cut(s) 1400, 1619, 1809, 2006, 2850, 3024
BseRI GAGGAG 2 cut(s) 888, 2798
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 6 cut(s) 657, 985, 1845, 2349, 2435, 3468
BseYI CCCAGC 1 cut(s) 1939
BsgI GTGCAG 2 cut(s) 1177, 2036
Bsh1236I CGCG 4 cut(s) 293, 1673, 2231, 2608
BshFI GGCC 4 cut(s) 639, 884, 2828, 2958
BsiHKAI GWGCWC 3 cut(s) 539, 3032, 3312
BsiHKCI CYCGRG 2 cut(s) 1695, 1802
BsiSI CCGG 6 cut(s) 999, 1153, 1574, 1803, 1871, 2704
BslFI GGGAC 5 cut(s) 484, 1600, 1797, 1819, 3489
BslI CCNNNNNNNGG 6 cut(s) 880, 902, 1913, 1945, 3425, 3485
BsmAI GTCTC 4 cut(s) 305, 1598, 2074, 3428
BsmFI GGGAC 5 cut(s) 484, 1600, 1797, 1819, 3489
BsmI GAATGC 3 cut(s) 1083, 2405, 3064
BsnI GGCC 4 cut(s) 639, 884, 2828, 2958
Bso31I GGTCTC 1 cut(s) 3428
BsoBI CYCGRG 2 cut(s) 1695, 1802
Bsp119I TTCGAA 1 cut(s) 3056
Bsp1286I GDGCHC 4 cut(s) 539, 1766, 3032, 3312
Bsp13I TCCGGA 1 cut(s) 1573
Bsp1407I TGTACA 1 cut(s) 1267
Bsp19I CCATGG 1 cut(s) 1278
BspANI GGCC 4 cut(s) 639, 884, 2828, 2958
BspCNI CTCAG 6 cut(s) 28, 747, 1439, 1524, 1594, 1900
BspEI TCCGGA 1 cut(s) 1573
BspFNI CGCG 4 cut(s) 293, 1673, 2231, 2608
BspHI TCATGA 2 cut(s) 493, 2836
BspLI GGNNCC 6 cut(s) 969, 1807, 2344, 2466, 2702, 3477
BspMAI CTGCAG 1 cut(s) 1863
BspPI GGATC 9 cut(s) 200, 263, 1896, 2058, 2216, 2459, 2472, 2781, 2880
BspQI GCTCTTC 1 cut(s) 2724
BspT104I TTCGAA 1 cut(s) 3056
BspTI CTTAAG 1 cut(s) 2735
BspTNI GGTCTC 1 cut(s) 3428
BsrDI GCAATG 4 cut(s) 665, 1964, 2959, 3148
BsrFI RCCGGY 1 cut(s) 1152
BsrGI TGTACA 1 cut(s) 1267
BsrI ACTGG 6 cut(s) 1400, 1619, 1809, 2006, 2850, 3024
BssAI RCCGGY 1 cut(s) 1152
BssECI CCNNGG 7 cut(s) 749, 997, 1072, 1278, 1654, 1802, 1944
BssNAI GTATAC 1 cut(s) 1150
BssNI GRCGYC 1 cut(s) 1704
BssT1I CCWWGG 2 cut(s) 1278, 1944
Bst1107I GTATAC 1 cut(s) 1150
Bst2UI CCWGG 8 cut(s) 628, 903, 1113, 1655, 2261, 2469, 2648, 3269
Bst4CI ACNGT 9 cut(s) 188, 242, 750, 938, 1504, 1776, 1848, 2888, 3485
Bst6I CTCTTC 2 cut(s) 2724, 3345
BstACI GRCGYC 1 cut(s) 1704
BstAFI CTTAAG 1 cut(s) 2735
BstAPI GCANNNNNTGC 1 cut(s) 2411
BstAUI TGTACA 1 cut(s) 1267
BstBAI YACGTR 1 cut(s) 704
BstBI TTCGAA 1 cut(s) 3056
BstC8I GCNNGC 2 cut(s) 1747, 3064
BstDEI CTNAG 8 cut(s) 15, 734, 1413, 1426, 1511, 1602, 1841, 1908
BstDSI CCRYGG 2 cut(s) 749, 1278
BstENI CCTNNNNNAGG 2 cut(s) 900, 1911
BstFNI CGCG 4 cut(s) 293, 1673, 2231, 2608
BstHHI GCGC 1 cut(s) 1673
BstMAI GTCTC 4 cut(s) 305, 1598, 2074, 3428
BstMWI GCNNNNNNNGC 7 cut(s) 428, 1200, 1539, 1968, 2411, 2420, 2450
BstNI CCWGG 8 cut(s) 628, 903, 1113, 1655, 2261, 2469, 2648, 3269
BstNSI RCATGY 5 cut(s) 426, 826, 2170, 2493, 3111
BstSFI CTRYAG 1 cut(s) 1859
BstSLI GKGCMC 1 cut(s) 539
BstUI CGCG 4 cut(s) 293, 1673, 2231, 2608
BstV1I GCAGC 6 cut(s) 657, 985, 1845, 2349, 2435, 3468
BstV2I GAAGAC 4 cut(s) 821, 1011, 1074, 3320
BstX2I RGATCY 6 cut(s) 205, 268, 2102, 2371, 2464, 2773
BstXI CCANNNNNNTGG 3 cut(s) 806, 2461, 2770
BstYI RGATCY 6 cut(s) 205, 268, 2102, 2371, 2464, 2773
BstZ17I GTATAC 1 cut(s) 1150
BsuI GTATCC 2 cut(s) 294, 3220
BsuRI GGCC 4 cut(s) 639, 884, 2828, 2958
BtgI CCRYGG 2 cut(s) 749, 1278
BtgZI GCGATG 1 cut(s) 315
BtsI GCAGTG 4 cut(s) 781, 1189, 2024, 3373
BtsIMutI CAGTG 9 cut(s) 193, 537, 781, 1189, 1359, 1500, 2024, 3373, 3490
Cac8I GCNNGC 2 cut(s) 1747, 3064
CciI TCATGA 2 cut(s) 493, 2836
CfoI GCGC 1 cut(s) 1673
Cfr10I RCCGGY 1 cut(s) 1152
Cfr13I GGNCC 5 cut(s) 968, 1806, 2342, 2957, 3476
Cfr9I CCCGGG 1 cut(s) 1802
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 8 cut(s) 84, 442, 1268, 1622, 1864, 2899, 3135, 3450
CviQI GTAC 8 cut(s) 84, 442, 1268, 1622, 1864, 2899, 3135, 3450
DdeI CTNAG 8 cut(s) 15, 734, 1413, 1426, 1511, 1602, 1841, 1908
DrdI GACNNNNNNGTC 2 cut(s) 1612, 3388
DriI GACNNNNNGTC 1 cut(s) 1850
DseDI GACNNNNNNGTC 2 cut(s) 1612, 3388
EaeI YGGCCR 1 cut(s) 2826
Eam1104I CTCTTC 2 cut(s) 2724, 3345
Eam1105I GACNNNNNGTC 1 cut(s) 1850
EarI CTCTTC 2 cut(s) 2724, 3345
Eco130I CCWWGG 2 cut(s) 1278, 1944
Eco24I GRGCYC 1 cut(s) 1766
Eco31I GGTCTC 1 cut(s) 3428
Eco47I GGWCC 4 cut(s) 968, 1806, 2342, 3476
Eco57I CTGAAG 3 cut(s) 552, 2450, 2946
Eco88I CYCGRG 2 cut(s) 1695, 1802
EcoNI CCTNNNNNAGG 2 cut(s) 900, 1911
EcoRI GAATTC 2 cut(s) 2434, 3173
EcoRII CCWGG 8 cut(s) 626, 901, 1111, 1653, 2259, 2467, 2646, 3267
EcoT14I CCWWGG 2 cut(s) 1278, 1944
EcoT22I ATGCAT 1 cut(s) 2574
EcoT38I GRGCYC 1 cut(s) 1766
ErhI CCWWGG 2 cut(s) 1278, 1944
FalI AAGNNNNNCTT 8 cut(s) 1877, 1909, 2718, 2750, 2729, 2761, 3371, 3403
FaqI GGGAC 5 cut(s) 484, 1600, 1797, 1819, 3489
FauI CCCGC 1 cut(s) 2982
FbaI TGATCA 5 cut(s) 541, 1363, 3070, 3094, 3289
FblI GTMKAC 1 cut(s) 1149
Fnu4HI GCNGC 6 cut(s) 671, 974, 1859, 2363, 2424, 3457
FriOI GRGCYC 1 cut(s) 1766
Fsp4HI GCNGC 6 cut(s) 671, 974, 1859, 2363, 2424, 3457
FspBI CTAG 7 cut(s) 1145, 2379, 2576, 2814, 3191, 3447, 3453
GlaI GCGC 1 cut(s) 1672
GluI GCNGC 6 cut(s) 671, 974, 1859, 2363, 2424, 3457
GsaI CCCAGC 1 cut(s) 1943
GsuI CTGGAG 7 cut(s) 885, 937, 1095, 1383, 2243, 2669, 3290
HaeIII GGCC 4 cut(s) 639, 884, 2828, 2958
HapII CCGG 6 cut(s) 999, 1153, 1574, 1803, 1871, 2704
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 1 cut(s) 1673
Hin1I GRCGYC 1 cut(s) 1704
Hin6I GCGC 1 cut(s) 1671
HinP1I GCGC 1 cut(s) 1671
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HindIII AAGCTT 1 cut(s) 1089
HpaI GTTAAC 1 cut(s) 934
HpaII CCGG 6 cut(s) 999, 1153, 1574, 1803, 1871, 2704
HphI GGTGA 7 cut(s) 473, 1013, 1088, 2471, 3089, 3151, 3443
Hpy166II GTNNAC 7 cut(s) 481, 537, 756, 934, 1150, 1268, 1584
Hpy8I GTNNAC 7 cut(s) 481, 537, 756, 934, 1150, 1268, 1584
HpyAV CCTTC 7 cut(s) 629, 895, 1701, 2191, 2428, 2970, 3063
HpyCH4III ACNGT 9 cut(s) 188, 242, 750, 938, 1504, 1776, 1848, 2888, 3485
HpyCH4IV ACGT 4 cut(s) 703, 1704, 2897, 3423
HpyF10VI GCNNNNNNNGC 7 cut(s) 428, 1200, 1539, 1968, 2411, 2420, 2450
HpyF3I CTNAG 8 cut(s) 15, 734, 1413, 1426, 1511, 1602, 1841, 1908
HpySE526I ACGT 4 cut(s) 703, 1704, 2897, 3423
Hsp92I GRCGYC 1 cut(s) 1704
HspAI GCGC 1 cut(s) 1671
Kpn2I TCCGGA 1 cut(s) 1573
Ksp22I TGATCA 5 cut(s) 541, 1363, 3070, 3094, 3289
KspAI GTTAAC 1 cut(s) 934
LguI GCTCTTC 1 cut(s) 2724
LmnI GCTCC 3 cut(s) 4, 2262, 2675
Lsp1109I GCAGC 6 cut(s) 657, 985, 1845, 2349, 2435, 3468
MaeI CTAG 7 cut(s) 1145, 2379, 2576, 2814, 3191, 3447, 3453
MaeII ACGT 4 cut(s) 703, 1704, 2897, 3423
MaeIII GTNAC 4 cut(s) 245, 1498, 2182, 2492
MfeI CAATTG 1 cut(s) 2658
MflI RGATCY 6 cut(s) 205, 268, 2102, 2371, 2464, 2773
MhlI GDGCHC 4 cut(s) 539, 1766, 3032, 3312
MlyI GAGTC 2 cut(s) 466, 1600
MmeI TCCRAC 2 cut(s) 1227, 2751
Mph1103I ATGCAT 1 cut(s) 2574
MroI TCCGGA 1 cut(s) 1573
MroXI GAANNNNTTC 2 cut(s) 1823, 2559
MslI CAYNNNNRTG 4 cut(s) 186, 1896, 2952, 3402
MspA1I CMGCKG 1 cut(s) 2629
MspCI CTTAAG 1 cut(s) 2735
MspI CCGG 6 cut(s) 999, 1153, 1574, 1803, 1871, 2704
MunI CAATTG 1 cut(s) 2658
Mva1269I GAATGC 3 cut(s) 1083, 2405, 3064
MvaI CCWGG 8 cut(s) 628, 903, 1113, 1655, 2261, 2469, 2648, 3269
MvnI CGCG 4 cut(s) 293, 1673, 2231, 2608
MwoI GCNNNNNNNGC 7 cut(s) 428, 1200, 1539, 1968, 2411, 2420, 2450
NciI CCSGG 4 cut(s) 999, 1803, 1804, 1872
NcoI CCATGG 1 cut(s) 1278
NlaIV GGNNCC 6 cut(s) 969, 1807, 2344, 2466, 2702, 3477
NmeAIII GCCGAG 1 cut(s) 425
NmuCI GTSAC 4 cut(s) 245, 1498, 2182, 2492
NsiI ATGCAT 1 cut(s) 2574
NspI RCATGY 5 cut(s) 426, 826, 2170, 2493, 3111
NspV TTCGAA 1 cut(s) 3056
PaeR7I CTCGAG 1 cut(s) 1695
PagI TCATGA 2 cut(s) 493, 2836
PciI ACATGT 2 cut(s) 2489, 3107
PciSI GCTCTTC 1 cut(s) 2724
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 3 cut(s) 1083, 2405, 3064
PdmI GAANNNNTTC 2 cut(s) 1823, 2559
PfoI TCCNGGA 2 cut(s) 2259, 2467
PkrI GCNGC 6 cut(s) 672, 975, 1860, 2364, 2425, 3458
PleI GAGTC 2 cut(s) 466, 1600
PpsI GAGTC 2 cut(s) 466, 1600
Ppu21I YACGTR 1 cut(s) 704
PscI ACATGT 2 cut(s) 2489, 3107
Psp6I CCWGG 8 cut(s) 626, 901, 1111, 1653, 2259, 2467, 2646, 3267
PspFI CCCAGC 1 cut(s) 1939
PspGI CCWGG 8 cut(s) 626, 901, 1111, 1653, 2259, 2467, 2646, 3267
PspN4I GGNNCC 6 cut(s) 969, 1807, 2344, 2466, 2702, 3477
PspPI GGNCC 5 cut(s) 968, 1806, 2342, 2957, 3476
PstI CTGCAG 1 cut(s) 1863
PsuI RGATCY 6 cut(s) 205, 268, 2102, 2371, 2464, 2773
PvuII CAGCTG 1 cut(s) 2629
RsaI GTAC 8 cut(s) 85, 443, 1269, 1623, 1865, 2900, 3136, 3451
RsaNI GTAC 8 cut(s) 84, 442, 1268, 1622, 1864, 2899, 3135, 3450
RseI CAYNNNNRTG 4 cut(s) 186, 1896, 2952, 3402
SapI GCTCTTC 1 cut(s) 2724
SatI GCNGC 6 cut(s) 671, 974, 1859, 2363, 2424, 3457
Sau96I GGNCC 5 cut(s) 968, 1806, 2342, 2957, 3476
ScaI AGTACT 2 cut(s) 85, 3451
SchI GAGTC 2 cut(s) 466, 1600
SduI GDGCHC 4 cut(s) 539, 1766, 3032, 3312
SfcI CTRYAG 1 cut(s) 1859
Sfr274I CTCGAG 1 cut(s) 1695
SfuI TTCGAA 1 cut(s) 3056
SinI GGWCC 4 cut(s) 968, 1806, 2342, 3476
SlaI CTCGAG 1 cut(s) 1695
SmaI CCCGGG 1 cut(s) 1804
SmiMI CAYNNNNRTG 4 cut(s) 186, 1896, 2952, 3402
SmlI CTYRAG 5 cut(s) 114, 1451, 1695, 2735, 3311
SmoI CTYRAG 5 cut(s) 114, 1451, 1695, 2735, 3311
SpeI ACTAGT 1 cut(s) 2813
SspI AATATT 3 cut(s) 221, 684, 1715
SspMI CTAG 7 cut(s) 1145, 2379, 2576, 2814, 3191, 3447, 3453
StyI CCWWGG 2 cut(s) 1278, 1944
TaaI ACNGT 9 cut(s) 188, 242, 750, 938, 1504, 1776, 1848, 2888, 3485
TaiI ACGT 4 cut(s) 706, 1707, 2900, 3426
TaqI TCGA 8 cut(s) 378, 527, 1051, 1696, 2119, 2325, 2348, 3056
TaqII GACCGA 3 cut(s) 1486, 2286, 3452
TatI WGTACW 5 cut(s) 83, 441, 1267, 1863, 3449
TscAI CASTG 9 cut(s) 193, 544, 781, 1196, 1366, 1507, 2024, 3380, 3490
TseFI GTSAC 4 cut(s) 245, 1498, 2182, 2492
TseI GCWGC 6 cut(s) 670, 973, 1858, 2362, 2423, 3456
Tsp45I GTSAC 4 cut(s) 245, 1498, 2182, 2492
TspGWI ACGGA 2 cut(s) 919, 3152
TspMI CCCGGG 1 cut(s) 1802
TspRI CASTG 9 cut(s) 193, 544, 781, 1196, 1366, 1507, 2024, 3380, 3490
Vha464I CTTAAG 1 cut(s) 2735
VneI GTGCAC 1 cut(s) 535
VpaK11BI GGWCC 4 cut(s) 968, 1806, 2342, 3476
XagI CCTNNNNNAGG 2 cut(s) 900, 1911
XapI RAATTY 9 cut(s) 102, 461, 613, 1333, 1569, 1823, 2434, 2558, 3173
XbaI TCTAGA 1 cut(s) 2378
XceI RCATGY 5 cut(s) 426, 826, 2170, 2493, 3111
XcmI CCANNNNNNNNNTGG 2 cut(s) 961, 2804
XhoI CTCGAG 1 cut(s) 1695
XmaI CCCGGG 1 cut(s) 1802
XmiI GTMKAC 1 cut(s) 1149
XmnI GAANNNNTTC 2 cut(s) 1823, 2559
XspI CTAG 7 cut(s) 1145, 2379, 2576, 2814, 3191, 3447, 3453
ZraI GACGTC 1 cut(s) 1705
ZrmI AGTACT 2 cut(s) 85, 3451
Zsp2I ATGCAT 1 cut(s) 2574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.