Rmu_co8025080.1_g000001

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8025080.1
OG0001545
Physical Location & Seq
Forward (+)
1 .. 542
542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8025080.1_g000001.1.cds

Sequence Viewer

Length: 477 bp
aatggttcgagcttcaaagaaatggaccctcgataacaatcctgctgccaccagatctatctagagatgctaattggataggattggcattctgtgcatcctttgcagctctgaagaattcaattgccaactttgccaataatgtggatccaggacttcctcaccaccttacatgtcacttggaaaaggatgatggaggtagtctggaatctctccatgactattgcaccaccaatgaagaatttcaatggatgcatctaggaggatttatgtggctctcctatataccgcgaacatggttttcagatcagctgaatgaatgtggttgcctggaggcttcaattgtaagtggtcatggagcatttaatgtggataagtgtgggttccggcttgtatatcagcaagatgaagaagagcttaagcagaccttaatgctttgcaagaccgctttgttggatctttatgaggagaagcaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

158

Amino Acids

17.95

Weight (kDa)

4.69

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 291
AciI CCGC 2 cut(s) 289, 446
AclWI GGATC 3 cut(s) 142, 155, 464
AcsI RAATTY 2 cut(s) 117, 241
AcuI CTGAAG 1 cut(s) 133
AflII CTTAAG 1 cut(s) 418
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 4 cut(s) 16, 122, 247, 341
AjnI CCWGG 2 cut(s) 150, 329
AluBI AGCT 4 cut(s) 12, 109, 312, 417
AluI AGCT 4 cut(s) 12, 109, 312, 417
AlwI GGATC 3 cut(s) 142, 155, 464
ApeKI GCWGC 2 cut(s) 45, 106
ApoI RAATTY 2 cut(s) 117, 241
Asp700I GAANNNNTTC 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 25
BamHI GGATCC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 32, 118
BccI CCATC 1 cut(s) 187
BciT130I CCWGG 2 cut(s) 152, 331
BfaI CTAG 2 cut(s) 62, 259
BfrI CTTAAG 1 cut(s) 418
BglII AGATCT 1 cut(s) 54
BisI GCNGC 2 cut(s) 46, 107
BlsI GCNGC 2 cut(s) 47, 108
Bme1390I CCNGG 2 cut(s) 152, 331
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 3 cut(s) 27, 149, 385
BmrFI CCNGG 2 cut(s) 152, 331
BmsI GCATC 4 cut(s) 57, 106, 242, 264
BpmI CTGGAG 1 cut(s) 352
BsaBI GATNNNNATC 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 37
BseBI CCWGG 2 cut(s) 152, 331
BseGI GGATG 3 cut(s) 97, 195, 257
BseJI GATNNNNATC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 32, 118
Bsh1236I CGCG 1 cut(s) 291
BsiSI CCGG 1 cut(s) 387
BsmI GAATGC 1 cut(s) 88
Bsp143I GATC 4 cut(s) 54, 147, 306, 456
BspACI CCGC 2 cut(s) 289, 446
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 3 cut(s) 27, 149, 385
BspPI GGATC 3 cut(s) 142, 155, 464
BspQI GCTCTTC 1 cut(s) 407
BspTI CTTAAG 1 cut(s) 418
BssMI GATC 4 cut(s) 54, 147, 306, 456
Bst2UI CCWGG 2 cut(s) 152, 331
Bst6I CTCTTC 1 cut(s) 407
BstAFI CTTAAG 1 cut(s) 418
BstAPI GCANNNNNTGC 2 cut(s) 94, 103
BstF5I GGATG 3 cut(s) 97, 195, 257
BstFNI CGCG 1 cut(s) 291
BstKTI GATC 4 cut(s) 57, 150, 309, 459
BstMBI GATC 4 cut(s) 54, 147, 306, 456
BstMWI GCNNNNNNNGC 3 cut(s) 94, 103, 133
BstNI CCWGG 2 cut(s) 152, 331
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 150, 329
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 32, 118
BstX2I RGATCY 3 cut(s) 54, 147, 456
BstXI CCANNNNNNTGG 1 cut(s) 144
BstYI RGATCY 3 cut(s) 54, 147, 456
BtsCI GGATG 3 cut(s) 97, 195, 257
Cfr13I GGNCC 1 cut(s) 25
CviAII CATG 4 cut(s) 173, 217, 296, 355
CviJI RGCY 7 cut(s) 12, 109, 276, 312, 337, 390, 417
CviKI_1 RGCY 7 cut(s) 12, 109, 276, 312, 337, 390, 417
DpnI GATC 4 cut(s) 56, 149, 308, 458
DpnII GATC 4 cut(s) 54, 147, 306, 456
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 150, 329
EcoT22I ATGCAT 1 cut(s) 257
FaeI CATG 4 cut(s) 176, 220, 299, 358
FaiI YATR 9 cut(s) 174, 218, 271, 284, 286, 297, 356, 396, 464
FalI AAGNNNNNCTT 4 cut(s) 401, 433, 412, 444
FatI CATG 4 cut(s) 172, 216, 295, 354
Fnu4HI GCNGC 2 cut(s) 46, 107
FokI GGATG 3 cut(s) 84, 202, 264
Fsp4HI GCNGC 2 cut(s) 46, 107
FspBI CTAG 2 cut(s) 62, 259
GluI GCNGC 2 cut(s) 46, 107
GsuI CTGGAG 1 cut(s) 352
HapII CCGG 1 cut(s) 387
Hin1II CATG 4 cut(s) 176, 220, 299, 358
HinfI GANTC 1 cut(s) 208
HpaII CCGG 1 cut(s) 387
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 113, 306
Hpy188III TCNNGA 2 cut(s) 62, 205
HpyCH4V TGCA 5 cut(s) 97, 106, 227, 255, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 103, 133
Hsp92II CATG 4 cut(s) 176, 220, 299, 358
Kzo9I GATC 4 cut(s) 54, 147, 306, 456
LguI GCTCTTC 1 cut(s) 407
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 8 cut(s) 55, 65, 137, 164, 190, 316, 343, 400
Lsp1109I GCAGC 2 cut(s) 32, 118
LweI GCATC 4 cut(s) 57, 106, 242, 264
MaeI CTAG 2 cut(s) 62, 259
MaeIII GTNAC 1 cut(s) 175
MalI GATC 4 cut(s) 56, 149, 308, 458
MboI GATC 4 cut(s) 54, 147, 306, 456
MboII GAAGA 4 cut(s) 126, 250, 421, 424
MfeI CAATTG 2 cut(s) 122, 341
MflI RGATCY 3 cut(s) 54, 147, 456
MluCI AATT 5 cut(s) 72, 117, 122, 241, 341
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 6 cut(s) 39, 170, 190, 256, 327, 459
Mph1103I ATGCAT 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 242
MseI TTAA 3 cut(s) 365, 419, 430
MspA1I CMGCKG 1 cut(s) 312
MspCI CTTAAG 1 cut(s) 418
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 2 cut(s) 152, 331
MunI CAATTG 2 cut(s) 122, 341
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 2 cut(s) 152, 331
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 94, 103, 133
NdeII GATC 4 cut(s) 54, 147, 306, 456
NlaIII CATG 4 cut(s) 176, 220, 299, 358
NlaIV GGNNCC 3 cut(s) 27, 149, 385
NmuCI GTSAC 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 1 cut(s) 176
PciI ACATGT 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 407
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 2 cut(s) 47, 108
PscI ACATGT 1 cut(s) 172
Psp6I CCWGG 2 cut(s) 150, 329
PspGI CCWGG 2 cut(s) 150, 329
PspN4I GGNNCC 3 cut(s) 27, 149, 385
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 3 cut(s) 54, 147, 456
PvuII CAGCTG 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 407
SaqAI TTAA 3 cut(s) 365, 419, 430
SatI GCNGC 2 cut(s) 46, 107
Sau3AI GATC 4 cut(s) 54, 147, 306, 456
Sau96I GGNCC 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 152, 331
SetI ASST 7 cut(s) 14, 111, 170, 201, 314, 419, 430
SfaNI GCATC 4 cut(s) 57, 106, 242, 264
SinI GGWCC 1 cut(s) 25
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
Sse9I AATT 5 cut(s) 72, 117, 122, 241, 341
SsiI CCGC 2 cut(s) 289, 446
SspMI CTAG 2 cut(s) 62, 259
StyD4I CCNGG 2 cut(s) 150, 329
TaqI TCGA 2 cut(s) 8, 31
TasI AATT 5 cut(s) 72, 117, 122, 241, 341
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 3 cut(s) 365, 419, 430
Tru9I TTAA 3 cut(s) 365, 419, 430
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 45, 106
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 251, 332, 422
Vha464I CTTAAG 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 2 cut(s) 117, 241
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 2 cut(s) 62, 259
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.