Rh4BG299900

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001545
Physical Location & Seq
Forward (+)
47123178 .. 47124648
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG299900.1

Sequence Viewer

Length: 1383 bp
ATGCTTAAACAAATAGTCACAAGCTTTTTGAAGGGTTTTCAACAACAGGACTTTGCTGATCCCTCCGCGAACATACTATTGTATAAGAATCAGCTCCAGGACTTTGACCGTTGCTCAATCTATAATTCTTGTTTCCCCCCAAACGAGATTGTAGAATGGTTCGAGCTTCAAAGCAATGGACCCTCAATAACAATCCTGCTGCCACCAGATCTATATAGAGATGCTAATTGGATAGGATTGGCATTCTGTGCATCCTTTGCAGCTCTGAAGAATTCAAGTGCCAACTTTGCCAATAATGTGGATCCAGGACTTCCTCACCACCTTACATGTCACTTGGAAAAGGATGATGGAGGTAGTCTGGAATCTCTCCATGACTATTGCACCACCAATGAAGAATTTCAATGGATGCATCTAGGAGGATTTATGTGGCTCTCCTATATACCGCGAACATGGTTTTCGGATCAGCTGAATGAATGTGGTTGCCTAGAGGCTTCAATTGTAAGTGGTCATGGAGCATTTAGTGTGGATAAGTGTGGGTTCCGGTTTGTATATCAGCAAGATGAAGAAGAGCTTAAGCAGACCTTAATGCTTTGCAAGACCGCTTTGTTGGATCTTTATGAGGAGAAGCAAGACCATGAGGCAGATGGAACTAGCAGAACATTGGCCGATAATCATGATGGAGAAACCAGTAATGCAAATGGTATCAGGGATCGGGACTTTGACTGTGCCTTGACGTACAATTCTTGTTTCCCTCCAAGTGATATTGTAGAGTGGTTCGGGCATCATAGCAATGGCCCTTCAGTAAAAATCCCGCTGCCATCAAATCTATACAAGGAGAATACTAATTGGGTAGGACTGGCTCTGTGCGCATACTTTTCAGTTCTTGACCCTTCGAATGCCAGCCTTGATCATTTGGATTCTGAAAAACTTGATCACCACCTTACATGTTATTTGGAAACTGATAGCGGTAGTACGGACTATTTCCATTGCTACATCACCAAAAACAAAGAATTCAAATGGTTGCATCTAGGAGGATTCTTATGGGTATCCTACATACCACGAAGGTTATTGAGTTTGGATCATCAGTCAAATGAGTGCATTAACCTGGAGGCTTCTATTGGAAGTGATCATCAAGGCTTGGGGGTGCTCAAGTGTGGGCTTCGTCTTCTATATCAACATGATGTGGAAGAGTTCAAGAAAACCATTATGCACTGCAAGACCTCCTTGTCTGAACATTACAATGGAGAAAATAAGCCTGACGTTTGGGGTGAGACCGAATCATCTAGTACTAGCAGCAAGTGTGTCATTGGGGGACCTCACAGTGGTAAATTAGAAAGACCCCAAATTTATAAGGGTAAAGGAGTGCTGGTGTCTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

460

Amino Acids

51.96

Weight (kDa)

5.08

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 45 - 189 3.6e-10 C-JID domain
C-JID PF20160 251 - 396 8.4e-11 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1350
AasI GACNNNNNNGTC 1 cut(s) 1225
Acc16I TGCGCA 1 cut(s) 868
AccII CGCG 2 cut(s) 68, 445
AciI CCGC 5 cut(s) 66, 443, 600, 812, 966
AclWI GGATC 7 cut(s) 53, 296, 309, 468, 618, 717, 1086
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 4 cut(s) 271, 395, 1010, 1344
AcuI CTGAAG 2 cut(s) 287, 783
AfaI GTAC 3 cut(s) 737, 973, 1288
AfiI CCNNNNNNNGG 1 cut(s) 1322
AflII CTTAAG 1 cut(s) 572
AflIII ACRYGT 2 cut(s) 326, 944
AgsI TTSAA 8 cut(s) 31, 41, 170, 276, 401, 495, 1015, 1195
AjnI CCWGG 3 cut(s) 96, 304, 1104
AluBI AGCT 6 cut(s) 24, 94, 166, 263, 466, 571
AluI AGCT 6 cut(s) 24, 94, 166, 263, 466, 571
Alw21I GWGCWC 1 cut(s) 1149
Alw26I GTCTC 1 cut(s) 1265
AlwI GGATC 7 cut(s) 53, 296, 309, 468, 618, 717, 1086
AoxI GGCC 2 cut(s) 663, 793
ApeKI GCWGC 4 cut(s) 199, 260, 814, 1293
ApoI RAATTY 4 cut(s) 271, 395, 1010, 1344
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 3 cut(s) 179, 794, 1313
AsuHPI GGTGA 4 cut(s) 308, 926, 988, 1280
AsuII TTCGAA 1 cut(s) 893
AvaII GGWCC 2 cut(s) 179, 1313
BamHI GGATCC 1 cut(s) 301
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 1 cut(s) 1149
BbvI GCAGC 4 cut(s) 186, 272, 801, 1305
BccI CCATC 4 cut(s) 341, 638, 671, 826
BciT130I CCWGG 3 cut(s) 98, 306, 1106
BciVI GTATCC 1 cut(s) 1057
BclI TGATCA 3 cut(s) 907, 931, 1126
BcoDI GTCTC 1 cut(s) 1265
BfaI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
BfrI CTTAAG 1 cut(s) 572
BfuI GTATCC 1 cut(s) 1057
BglII AGATCT 1 cut(s) 208
BisI GCNGC 4 cut(s) 200, 261, 815, 1294
BlsI GCNGC 4 cut(s) 201, 262, 816, 1295
BmcAI AGTACT 1 cut(s) 1288
Bme1390I CCNGG 3 cut(s) 98, 306, 1106
Bme18I GGWCC 2 cut(s) 179, 1313
BmgT120I GGNCC 3 cut(s) 179, 794, 1313
BmiI GGNNCC 4 cut(s) 181, 303, 539, 1314
BmrFI CCNGG 3 cut(s) 98, 306, 1106
BmsI GCATC 6 cut(s) 211, 260, 396, 418, 790, 1033
BpiI GAAGAC 1 cut(s) 1157
BpmI CTGGAG 2 cut(s) 80, 1127
Bpu14I TTCGAA 1 cut(s) 893
BpuEI CTTGAG 1 cut(s) 1133
BsaI GGTCTC 1 cut(s) 1265
BsaWI WCCGGW 1 cut(s) 540
BsaXI ACNNNNNCTCC 2 cut(s) 1353, 1383
Bsc4I CCNNNNNNNGG 1 cut(s) 1322
Bse1I ACTGG 2 cut(s) 687, 861
Bse3DI GCAATG 3 cut(s) 181, 796, 985
BseBI CCWGG 3 cut(s) 98, 306, 1106
BseGI GGATG 3 cut(s) 251, 349, 411
BseLI CCNNNNNNNGG 1 cut(s) 1322
BseMI GCAATG 3 cut(s) 181, 796, 985
BseNI ACTGG 2 cut(s) 687, 861
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 4 cut(s) 186, 272, 801, 1305
Bsh1236I CGCG 2 cut(s) 68, 445
BshFI GGCC 2 cut(s) 665, 795
BsiHKAI GWGCWC 1 cut(s) 1149
BsiSI CCGG 1 cut(s) 541
BslFI GGGAC 2 cut(s) 728, 1326
BslI CCNNNNNNNGG 1 cut(s) 1322
BsmAI GTCTC 1 cut(s) 1265
BsmFI GGGAC 2 cut(s) 728, 1326
BsmI GAATGC 2 cut(s) 242, 901
BsnI GGCC 2 cut(s) 665, 795
Bso31I GGTCTC 1 cut(s) 1265
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 1149
BspACI CCGC 5 cut(s) 66, 443, 600, 812, 966
BspANI GGCC 2 cut(s) 665, 795
BspFNI CGCG 2 cut(s) 68, 445
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 4 cut(s) 181, 303, 539, 1314
BspPI GGATC 7 cut(s) 53, 296, 309, 468, 618, 717, 1086
BspQI GCTCTTC 1 cut(s) 561
BspT104I TTCGAA 1 cut(s) 893
BspTI CTTAAG 1 cut(s) 572
BspTNI GGTCTC 1 cut(s) 1265
BsrDI GCAATG 3 cut(s) 181, 796, 985
BsrI ACTGG 2 cut(s) 687, 861
Bst2UI CCWGG 3 cut(s) 98, 306, 1106
Bst4CI ACNGT 3 cut(s) 110, 725, 1322
Bst6I CTCTTC 2 cut(s) 561, 1182
BstAFI CTTAAG 1 cut(s) 572
BstAPI GCANNNNNTGC 2 cut(s) 248, 257
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 1 cut(s) 901
BstF5I GGATG 3 cut(s) 251, 349, 411
BstFNI CGCG 2 cut(s) 68, 445
BstHHI GCGC 1 cut(s) 869
BstMAI GTCTC 1 cut(s) 1265
BstMWI GCNNNNNNNGC 4 cut(s) 248, 257, 287, 866
BstNI CCWGG 3 cut(s) 98, 306, 1106
BstNSI RCATGY 2 cut(s) 330, 948
BstSCI CCNGG 3 cut(s) 96, 304, 1104
BstUI CGCG 2 cut(s) 68, 445
BstV1I GCAGC 4 cut(s) 186, 272, 801, 1305
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 3 cut(s) 208, 301, 610
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 3 cut(s) 208, 301, 610
BsuI GTATCC 1 cut(s) 1057
BsuRI GGCC 2 cut(s) 665, 795
BtsCI GGATG 3 cut(s) 251, 349, 411
BtsI GCAGTG 1 cut(s) 1210
BtsIMutI CAGTG 2 cut(s) 1210, 1327
Cac8I GCNNGC 1 cut(s) 901
CciI TCATGA 1 cut(s) 673
CfoI GCGC 1 cut(s) 869
Cfr13I GGNCC 3 cut(s) 179, 794, 1313
Csp6I GTAC 3 cut(s) 736, 972, 1287
CviAII CATG 8 cut(s) 327, 371, 450, 509, 635, 674, 945, 1178
CviQI GTAC 3 cut(s) 736, 972, 1287
DrdI GACNNNNNNGTC 1 cut(s) 1225
DseDI GACNNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 561, 1182
EarI CTCTTC 2 cut(s) 561, 1182
Eco31I GGTCTC 1 cut(s) 1265
Eco47I GGWCC 2 cut(s) 179, 1313
Eco57I CTGAAG 2 cut(s) 287, 783
EcoO109I RGGNCCY 1 cut(s) 1313
EcoRI GAATTC 2 cut(s) 271, 1010
EcoRII CCWGG 3 cut(s) 96, 304, 1104
EcoT22I ATGCAT 1 cut(s) 411
FaeI CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
FalI AAGNNNNNCTT 6 cut(s) 555, 587, 566, 598, 1208, 1240
FaqI GGGAC 2 cut(s) 728, 1326
FatI CATG 8 cut(s) 326, 370, 449, 508, 634, 673, 944, 1177
FauI CCCGC 1 cut(s) 819
FbaI TGATCA 3 cut(s) 907, 931, 1126
Fnu4HI GCNGC 4 cut(s) 200, 261, 815, 1294
FokI GGATG 3 cut(s) 238, 356, 418
Fsp4HI GCNGC 4 cut(s) 200, 261, 815, 1294
FspAI RTGCGCAY 1 cut(s) 868
FspBI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
FspI TGCGCA 1 cut(s) 868
GlaI GCGC 1 cut(s) 868
GluI GCNGC 4 cut(s) 200, 261, 815, 1294
GsuI CTGGAG 2 cut(s) 80, 1127
HaeIII GGCC 2 cut(s) 665, 795
HapII CCGG 1 cut(s) 541
HhaI GCGC 1 cut(s) 869
Hin1II CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 5 cut(s) 88, 362, 917, 1035, 1277
HpaII CCGG 1 cut(s) 541
HphI GGTGA 4 cut(s) 308, 926, 988, 1280
Hpy188I TCNGA 4 cut(s) 267, 460, 922, 1231
Hpy188III TCNNGA 5 cut(s) 359, 674, 713, 884, 1195
HpyAV CCTTC 4 cut(s) 25, 807, 900, 1056
HpyCH4III ACNGT 3 cut(s) 110, 725, 1322
HpyCH4IV ACGT 2 cut(s) 734, 1260
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 257, 287, 866
HpySE526I ACGT 2 cut(s) 734, 1260
Hsp92II CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
HspAI GCGC 1 cut(s) 867
Ksp22I TGATCA 3 cut(s) 907, 931, 1126
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 99, 512
Lsp1109I GCAGC 4 cut(s) 186, 272, 801, 1305
LweI GCATC 6 cut(s) 211, 260, 396, 418, 790, 1033
MaeI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
MaeII ACGT 2 cut(s) 734, 1260
MaeIII GTNAC 2 cut(s) 16, 329
MboII GAAGA 6 cut(s) 280, 404, 575, 578, 1157, 1199
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 3 cut(s) 208, 301, 610
MhlI GDGCHC 1 cut(s) 1149
MmeI TCCRAC 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 4 cut(s) 6, 573, 584, 1101
MslI CAYNNNNRTG 2 cut(s) 789, 1239
MspA1I CMGCKG 2 cut(s) 466, 814
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 1 cut(s) 541
MspR9I CCNGG 3 cut(s) 98, 306, 1106
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 2 cut(s) 242, 901
MvaI CCWGG 3 cut(s) 98, 306, 1106
MvnI CGCG 2 cut(s) 68, 445
MwoI GCNNNNNNNGC 4 cut(s) 248, 257, 287, 866
NlaIII CATG 8 cut(s) 330, 374, 453, 512, 638, 677, 948, 1181
NlaIV GGNNCC 4 cut(s) 181, 303, 539, 1314
NmuCI GTSAC 2 cut(s) 16, 329
NsbI TGCGCA 1 cut(s) 868
NsiI ATGCAT 1 cut(s) 411
NspI RCATGY 2 cut(s) 330, 948
NspV TTCGAA 1 cut(s) 893
PagI TCATGA 1 cut(s) 673
PciI ACATGT 2 cut(s) 326, 944
PciSI GCTCTTC 1 cut(s) 561
PctI GAATGC 2 cut(s) 242, 901
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 5 cut(s) 88, 362, 917, 1035, 1277
PfoI TCCNGGA 2 cut(s) 96, 304
PkrI GCNGC 4 cut(s) 201, 262, 816, 1295
PpuMI RGGWCCY 1 cut(s) 1313
PscI ACATGT 2 cut(s) 326, 944
PsiI TTATAA 1 cut(s) 1350
Psp5II RGGWCCY 1 cut(s) 1313
Psp6I CCWGG 3 cut(s) 96, 304, 1104
PspGI CCWGG 3 cut(s) 96, 304, 1104
PspN4I GGNNCC 4 cut(s) 181, 303, 539, 1314
PspPI GGNCC 3 cut(s) 179, 794, 1313
PspPPI RGGWCCY 1 cut(s) 1313
PsuI RGATCY 3 cut(s) 208, 301, 610
PvuII CAGCTG 1 cut(s) 466
RsaI GTAC 3 cut(s) 737, 973, 1288
RsaNI GTAC 3 cut(s) 736, 972, 1287
RseI CAYNNNNRTG 2 cut(s) 789, 1239
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 4 cut(s) 6, 573, 584, 1101
SatI GCNGC 4 cut(s) 200, 261, 815, 1294
Sau96I GGNCC 3 cut(s) 179, 794, 1313
ScaI AGTACT 1 cut(s) 1288
ScrFI CCNGG 3 cut(s) 98, 306, 1106
SduI GDGCHC 1 cut(s) 1149
SfaNI GCATC 6 cut(s) 211, 260, 396, 418, 790, 1033
SfuI TTCGAA 1 cut(s) 893
SinI GGWCC 2 cut(s) 179, 1313
SmiMI CAYNNNNRTG 2 cut(s) 789, 1239
SmlI CTYRAG 2 cut(s) 572, 1148
SmoI CTYRAG 2 cut(s) 572, 1148
SsiI CCGC 5 cut(s) 66, 443, 600, 812, 966
SspMI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
StyD4I CCNGG 3 cut(s) 96, 304, 1104
TaaI ACNGT 3 cut(s) 110, 725, 1322
TaiI ACGT 2 cut(s) 737, 1263
TaqI TCGA 2 cut(s) 162, 893
TaqII GACCGA 1 cut(s) 1289
TatI WGTACW 1 cut(s) 1286
TfiI GAWTC 5 cut(s) 88, 362, 917, 1035, 1277
Tru1I TTAA 4 cut(s) 6, 573, 584, 1101
Tru9I TTAA 4 cut(s) 6, 573, 584, 1101
TscAI CASTG 2 cut(s) 1217, 1327
TseFI GTSAC 2 cut(s) 16, 329
TseI GCWGC 4 cut(s) 199, 260, 814, 1293
Tsp45I GTSAC 2 cut(s) 16, 329
TspDTI ATGAA 3 cut(s) 405, 486, 576
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 2 cut(s) 1217, 1327
Vha464I CTTAAG 1 cut(s) 572
VpaK11BI GGWCC 2 cut(s) 179, 1313
XapI RAATTY 4 cut(s) 271, 395, 1010, 1344
XceI RCATGY 2 cut(s) 330, 948
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 6 cut(s) 413, 485, 651, 1028, 1284, 1290
ZrmI AGTACT 1 cut(s) 1288
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.