Rmu_sc0010349.1_g000002

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010349.1
OG0001545
Physical Location & Seq
Reverse (-)
4468 .. 19640
15173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010349.1_g000002.1.cds

Sequence Viewer

Length: 8772 bp
atgcagaatgtcaatctgataaggagtagattgcgttacaaaagggtgcttatcattcttgatgatgtgaatgagttagaaacaaatagaggctgtagccggaaaggatggaaagctttcaagaaataccatcctatatatggttttgctgagttgtcttacagttttgtagattatgctgatggtgttcatcctttggctcttgtagttttgggttcattcctattgcacagaagcatagaagaatgttctgcaacagctcatttctttgaatctggaagttgtaaatgccttactactcttccccaccgtatcagcttgaatcttgaagttttcattctttcgggatgttgtaaacttgagaggcttccagagattggggaaaacatgaaacaattatcagaacttcatttagacgagacagctataaaagagctaccaacatcaattaagcatttgattggccttacgattctcaatctaaaagactgtaaaaatgtttccagtctgccggatgccatttgtactctgtcatcacttcaaattctcaacctttcaggctgctcaaatcttcaagagtttcctgaagacttggggagcttagaatgtttaaatgagcttgatgcaagcagaagtggtataagacaaacactttcctccattctacttctgaaaaaccttaaactactatcttttgctggatgtcaaggtgatcatctgcatgctccagcgaattcatggtgctcctttttgatcagttgttgcttgtggccgtcagattcagcaccctctaattgcaattccaattccattggcttgcaaattccgacttcattctcaggactatgctctttggagagattagatctaaggaattgcaatctatgggaaggagcaatccctgatgatattggctacttacccttgttgaactatctgaatttaagtggaaactattttgagagcctacctgatagtatttctaaactttctcagcttcaagagcttagattgaaagactgtagtaacctaaaagcattgccacaacttacatcaggactacgaagtgtatctgcacaagggtgtcctctccttggaacaaaagatggaatgcggtggacttcagcttcccatggatttacttatatacatttcgagctaattccttctcatgaaccaactccattcttcattgaaattgaacctcccggatatgattcaagttcaaccaagattgtcggtgaactgagaagaaacatagagttagttcttcaccaactctttgagggaggcaatgcaggttactatgattatggtttcactgtatctcctaaactcaaagcaattttgccgtggtttagtgatcaaagccttgggagcgaagtagtactactacctcattctcctccggatttgtacaaggacaagacatggctaggattatctgtctatgctgccttcactgtgtctccaggttcaacattttcctgtgcttgttacttgattaccgtcactggagaatattcggtacgtttgttagcaacatgcattgttgaagatgaatcttctggatcacagagaatctgtatcatccatatccgcgtccacgtttcccagaacaatggattcagtgtggtcaagtttttgctagctttgatgtccttattccaggtggaaaaaaggatgctgatatgtgtggggtccgtccgaacagagaagatgcaacctagcggcactagtactggaattacaattatggaggcaagttcggttgaaattgctaaatctcgcaactactccaagccattattggagaaatgtgagctgccacaaatagagcgattaactgttctgttttcaatggctgccccaagctcctcacgcttatctattccttcgaccaatcaatgcaaatttgatgtcttcctgagttttagaggcagtgacactcgcaaaaattttactgatcatttatacacagctttacatcagaaaggaatcctcgccttcaaggatgatgaagaactccaaagggggagagccatttcaccagaactctttaaagcaattgaagaatcaagaattgcagttgtcatattctcaagaaactatgcttcttccacctggtgtttggatgaacttgccaagattgttgattgcgctagtacctctggacttgttgtgttgccaatcttctatgacctgccaccaaccgaggtacgacagcagacaggaaattttgaggcagcgtttgctagacatgaactttccaaagacaacagggagaaggtgaaaacgtggaggcaagctttgactcaagtagcaaacctctctggatgggatttaaaagacaggcacgagacaaaggtcattgaagaaatcgtggaacatgtttctagtcatctgaatttcacattcccaacctgtcactccaaaaatattgttgggatgcatcgtgtgaaggaaattgaatcagacctaggtgtaggcaagttggatgaactacgtgttattggcatttgtggtatgcctggaattggtaagacaaccattgcacaagttgtttttgaaaatattcacaagaaatttgaagctagcagtttcattgctaatgttggagaggactatgaaaagaaaggtttgttccatcttcagaaacaactttttgatgacttgttgaagaccaaagtaaccacggcgatcgtcaataatgtgaggcagagattgcgtaacaagagggtgcttatcattcttgatgacgtgaaggagttggaacatatagtggacctagttggaaagggctctagtgaagagttcggtgcaggtagtagaatcatcataaccacaagagatgaaaagttgttggagaattatgggtccaaaatacacaaggttgaaaagcttactgaaagtgagtctcttctcctcttctgtcagaaagcctttaagaaagaccatcctgttgatgattttcttgagttgtcttacagttttacagattacgctaatggccttcctctggctcttgtagttttgggttcatacctataccgaagaaatgcgaaagactggtctgttgaaaagaaaagatggtctgatgaactgaaaaaattaagagatagcaacttttctggtccagaaaaaatattcaatattttaaaactaggtttttttggattagacatggcagcaaaggagatatttttggacattgcttgtttcttcaaaggagagaatgaaactgatgtaagaaggatatttgaaagttgtggcttgtatccagagtgttgtataccaagccttcttgagaaatccctgataactattgtaggaggaaaattgtggatgcacgagttactacaacaaactggccagcaaatagtccgtgaagaatccaaatggttaggggaacgcagcaggttgtgggatcatacagatattgttcctgtccttcagaacaataaggggacagaacttgttaaaggtattttccttagctcacctcaacaagccagagtgcacttgaaggcagaccctttctcagagatgcgcaacttaagactgctgaagattaagaatgtgatattgtctggatgccttgagttcactctttcggatctgttaagcttcttcgaatggcatggatatcctttaaaatttctgccctcaagttttagaccggataaacttgttgaattgcacttgcctcaaagccaaatagagcgactatgggaggaaaatatcaagcagcctctggaaatgttggtaattgtagaccttagtgactgtgaattcttgaccaagacccttgacttcagtcaagtcccgaagctggagaggctaatccttaaaggttgtaaaagattgtctgatattcacccatcaattggagatctcaaagggctcatttccttgaatctacagggttgtgaatctcttaccactcttccccccagtatcaagttgggatctctcatgactttcatactttcaggatgttccaaactgaaaagatttccggaaattggagaaaacatggaaaacttactggaacttcatttagatgggacagctatagtagagctaccaacatcaattaagcatttgactagcctctctctgctcaatttaaaagactgcaagctccttcagagtcttccggacgtaatttgtacgtctacatcactccaaactctcatccttcaaggctgctcaaatttatgccagttgccagaaaacttggggagcttagagtgtttgaaggtgcttgacataagcagaagtgctataaggcaggtaccttcctccattcaatttctgaaaaatcttacaggactatcttgcgccggatgtcgagatttgcctgctgaacgcagttcatggtgtttgccgaccttcatttcttgcttcttgccaccagatttagcatccgaaaattcctgtggtttgcagttgcctgcttcattgtccggtttatgctccttaagaaaattaattttaagcgattgcaatctgcttgaaggagcaatccctgatgacattggctgcatatcctcgttgaagtatctggatttaagtggaaacaactttgagacattaccccaaagcatctctcaactttatcagcttgaagaccttcggttaaatgattgtagaaacctaaaatcattgccaacatcacttcctataagtatacgaggtatattttcagataattgtcctctcctggatgaaacatttcaagaaatagtatggacttcaactgctttgggatttagctttataaattgcaaatacaacaaattttattcccttccgacagcggatcagcaccttaaccagcaaaatatccgaagatttttaatggagctattttctcagggtggttgttttcaatttttttttggggctgaaactgatattgcttcatggttctgtcgtcaaagtactaattcgtcaataaaaattccattacctcaagacttggatgggaaaagtcaatggattggttttgctctgtggtttcttattgaatcggaggtgcatgacagtgtggattcagaagaagatcctcaatccgaatatttcaacaagagaaattacagttgtgacatcttcacaaatgatgaagatcctcccctaaagtatccactcgcggattgcgatggtcgtgagacatatcagcatttgctttatataccagcagatcatttcgtgaaaaggttgaacaaatgcttcatcgaggctaaaatcaacatcaaaagaacaggggtggaagtggtagcatgtagagcgaggctattgtactcggcagatgtgccagaatttgtcgggactctggttcacctttggaatcatgaacaagtggggcatctggcaaatccatcatcgagcaagtgcactgatcagtcaaaagtttatccttttgcttcagaaacaagtttcttgattgaaattgaacgccagattttcagaaatttaagttcaaccaggatggaaggccatgaagtgagaacaagggtagaggcattgctgcttgaagaaatatttgagctggaatccgcacgtagctatgattatggtttcattttctctctcagagcaattttaccgtggtttactcatcaaagcatcggcagtgtagtcctttcactaaacgaccaaagcattgggggagtagtaggcatgtgtcttccaccacttttacatagcgataacgaatgggtagggttttccctatttgtggccttcactctgcctcctccaggtgttttggctgtaaagtattcttccgttaactgtcgtttgcttacctccagaggaataatgggtccgcagcataacctaacattagagttccccggtgaagacaactttatggggtcagagcgagttcttgttattcatatacctcgaaaacattttccagaagacctcggtgatcgcattcaagcttcttttgaatccctcactccaggtgtggaggttcaaatgtctgggatgcgtctagtataccggcaagatttgaatggcttaatgcaaactattactcactgcactctacgcagtccatttgcttacttcggacaagatgacaaaacatttatgttggaagaaaataagattggtgtacatttgaccatggagaaaagtttgcttcaacaagcaaaagacaggatgcttcaagattggcaaaattcttcaacaggttcaaaggtaatcactgatcaacaaattgagttgatgatactttctgaaactccagtcaatccacctaagaaaaaacattcagtaatcaagtggaaaagaaaccttgagcttttgcttcaatttttctttaggcttcctgttgaactatcggtcactgtatttctcagtcaaaacataatctcttgctcaaaacgctattctggtattttctatcaaaaggaaattccggagtggttcacagaacaacaatcatctgagactctggtgtccccagtacctacaaatcttggaaagggacaccatcccagacttgcttgcaattttcatttgagccctgaaataccgtgtagcttagaggttggcagcttacctgggaccagtcccaagaacaaattcatgtggttatatcttagacggtttgtctggctgcagtacataccgggtcattattttaaggatcacttgaatgaactccctgagttaggggtttcatttacgagtgattccccagccttggctgtaaagcaatgcggattccgcatcctatatgaccaagaggtgcaagaatactggaaagcaatagtgcagtgcgtcacttccttttttgacgacaatgaggatcatattcgccaattcattgaagatgagagacattcttttagcattacaagatcttttgggggttctcgacaacaggactttgatcgctcttttgagaaccatctccgaagcaacatgcttaaacaaatagtcacaagctttttgaagggttttcaacaacaggactttgatccctccgcgaacatactattgtataagaatcagctccagcaagatgaagaagagcttaagcagaccttaatgctttgcaagaccgctttgttggatctttatgaggagaagcaaggccatgaggcagatggaactagtagaacattggccgataatcatgatggagaaaccagtagtgcaaatggtatcaggaatcaggactttgactgtgccttgacgtacaattcttgcttccctccaagtgatattgtagagtggttcgacgacaatgaggatcatattcgccaattcattgaagatgagagacattcttttagcattacaagatcttttgggggttctcaacaacaggactttgatcgctcttttgagaaccatctccgaagcaacatgcttaaacaaatagtcacaaactttttgaagggttttcaacaacaggactttgatccctccgcgaacatactattgtataagaatcagctccaggactttgaccgatgctcaatctataattcttgtttccctccaaacgagattgcagaatggttcgagcttcaaagaaatggaccctcgataacaatcctgctgccaccagatctatctagagatgctaattggataggattggcattctgtgcatcctttgcagctctgaagaattcaattgccaactttgccaataatgtggatccaggacttcctcaccaccttacatgtcacttggaaaaggatgatggaggtagtctggaatctctccatgactattgcaccaccaatgaagaatttcaatggatgcatctaggaggatttatgtggctctcctatataccgcgaacatggttttcagatcagctgaatgaatgtggttgcctggaggcttcaattgtaagtggtcatggagcatttaatgtggataagtgtgggttccggcttgtatatcagcaagatgaagaagagcttaagcagaccttaatgctttgcaagaccgctttgttggatctttatgaggagaagcaaggccatgaggcagatggaactagtagaacattggccgataatcatgatggagaaaccagtagtgcaaatggtatcaggaatcaggactttgactgtgccttgacgtacaattcttgcttccctccaagtgatattgtagagtggttcgggcatcatagcaatggcccttcagtaaaaatcccgctaccatcaaatctatacaaggagaatactaattgggtaggactggctctgtgcgcatacttttcagttcttgacccttcgaatgccagccttgataatttggattctgaaaaacttgatcaccaccttacatgttatttggaaactgatagcggtagtacggactttttccattgctacatcaccaaaaacaaagaattcaaatggttgcatctaggaggattcttatgggtatcctacataccacaaaggttattgagtttgggtcatcagtcaaatgagtgcattaacctggaggcttctattggaagtgatcatcaaggcttgggggtgctcaagtgtgggcttcgtcttctctatgaacatgatgcggaggagttcaagaaaaccattatgcactgcaagacctccttgtctaaacattacaatggagaaaataagcctgacgttgtgggtgagaccgaatcatctagtactagcagcaagtgtgtcattgggggaccgcacagtggtatattagaaagaccccaaattgataagggtaaaggagtgctggtgtctggttggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

2923

Amino Acids

330.15

Weight (kDa)

5.62

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4784
AasI GACNNNNNNGTC 2 cut(s) 6475, 8614
Acc16I TGCGCA 2 cut(s) 3551, 8257
Acc36I ACCTGC 5 cut(s) 1289, 2211, 2813, 3408, 4285
Acc65I GGTACC 1 cut(s) 4297
AccB1I GGYRCC 1 cut(s) 4297
AccB7I CCANNNNNTGG 2 cut(s) 377, 3887
AccI GTMKAC 5 cut(s) 3292, 3774, 4178, 4693, 6012
AccII CGCG 5 cut(s) 1602, 5156, 7010, 7457, 7834
AccIII TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
AcoI YGGCCR 4 cut(s) 770, 3370, 7149, 8052
AdeI CACNNNGTG 1 cut(s) 2127
AflII CTTAAG 4 cut(s) 3556, 4483, 7058, 7961
AflIII ACRYGT 4 cut(s) 2389, 2506, 7715, 8333
AhdI GACNNNNNGTC 1 cut(s) 6629
AhlI ACTAGT 3 cut(s) 1736, 7136, 8039
AjiI CACGTC 1 cut(s) 2761
AjuI GAANNNNNNNTTGG 4 cut(s) 6108, 6140, 6587, 6619
AleI CACNNNNGTG 1 cut(s) 1081
Alw21I GWGCWC 4 cut(s) 746, 3522, 5402, 8538
Alw44I GTGCAC 2 cut(s) 3518, 5398
AlwNI CAGNNNCTG 1 cut(s) 3754
Aor13HI TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
ApaLI GTGCAC 2 cut(s) 3518, 5398
ArsI GACNNNNNNTTYG 4 cut(s) 1905, 1937, 3056, 3088
AseI ATTAAT 1 cut(s) 4493
Asp700I GAANNNNTTC 6 cut(s) 116, 2574, 4635, 4737, 6602, 7785
Asp718I GGTACC 1 cut(s) 4297
AspA2I CCTAGG 1 cut(s) 2479
AspLEI GCGC 4 cut(s) 2162, 3552, 4346, 8258
AspS9I GGNCC 9 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8183, 8702
AsuC2I CCSGG 3 cut(s) 1209, 5862, 6651
AsuII TTCGAA 2 cut(s) 3633, 8282
AsuNHI GCTAGC 2 cut(s) 1648, 2594
AvaII GGWCC 8 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8702
AvrII CCTAGG 1 cut(s) 2479
BaeGI GKGCMC 2 cut(s) 3522, 5402
BaeI ACNNNNGTAYC 2 cut(s) 1520, 1553
BalI TGGCCA 1 cut(s) 3372
BamHI GGATCC 1 cut(s) 7690
BanI GGYRCC 1 cut(s) 4297
BanII GRGCYC 3 cut(s) 2804, 3906, 6545
BarI GAAGNNNNNNTAC 2 cut(s) 1374, 1406
BauI CACGAG 2 cut(s) 2357, 3350
BbsI GAAGAC 9 cut(s) 594, 1915, 2687, 4148, 4638, 5684, 5874, 5937, 8546
Bbv12I GWGCWC 4 cut(s) 746, 3522, 5402, 8538
BceAI ACGGC 3 cut(s) 757, 1336, 2712
BciVI GTATCC 3 cut(s) 3288, 5157, 8446
BclI TGATCA 8 cut(s) 712, 753, 1363, 1966, 5404, 6226, 8320, 8515
BcnI CCSGG 3 cut(s) 1209, 5862, 6651
BcuI ACTAGT 3 cut(s) 1736, 7136, 8039
BfmI CTRYAG 5 cut(s) 94, 1021, 3920, 4074, 6638
BfrI CTTAAG 4 cut(s) 3556, 4483, 7058, 7961
BfuAI ACCTGC 5 cut(s) 1289, 2211, 2813, 3408, 4285
BfuI GTATCC 3 cut(s) 3288, 5157, 8446
BglII AGATCT 5 cut(s) 865, 3892, 6881, 7328, 7597
BlnI CCTAGG 1 cut(s) 2479
BmcAI AGTACT 4 cut(s) 1389, 1741, 4948, 8677
Bme18I GGWCC 8 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8702
BmeRI GACNNNNNGTC 1 cut(s) 6629
BmgBI CACGTC 1 cut(s) 2761
BmgT120I GGNCC 9 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8183, 8702
BmiI GGNNCC 9 cut(s) 1702, 2878, 4299, 5832, 6586, 7570, 7692, 7928, 8703
BmrI ACTGGG 2 cut(s) 3948, 6476
BmtI GCTAGC 2 cut(s) 1652, 2598
BmuI ACTGGG 2 cut(s) 3948, 6476
BoxI GACNNNNGTC 2 cut(s) 2366, 3816
BpiI GAAGAC 9 cut(s) 594, 1915, 2687, 4148, 4638, 5684, 5874, 5937, 8546
Bpu10I CCTNAGC 1 cut(s) 3494
Bpu14I TTCGAA 2 cut(s) 3633, 8282
BpuMI CCSGG 3 cut(s) 1209, 5862, 6651
BsaAI YACGTR 2 cut(s) 2507, 5567
BsaBI GATNNNNATC 3 cut(s) 4509, 5130, 7580
BsaI GGTCTC 1 cut(s) 8654
BsaWI WCCGGW 6 cut(s) 1408, 3679, 4018, 4159, 4469, 6436
BsaXI ACNNNNNCTCC 8 cut(s) 1312, 1342, 1453, 1483, 5743, 5773, 8742, 8772
Bse118I RCCGGY 1 cut(s) 6015
Bse8I GATNNNNATC 3 cut(s) 4509, 5130, 7580
BseAI TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
BseJI GATNNNNATC 3 cut(s) 4509, 5130, 7580
BseRI GAGGAG 7 cut(s) 1395, 1867, 2915, 5751, 7121, 8024, 8591
BseSI GKGCMC 2 cut(s) 3522, 5402
BseYI CCCAGC 1 cut(s) 6718
BsgI GTGCAG 4 cut(s) 1059, 2841, 6040, 6815
Bsh1236I CGCG 5 cut(s) 1602, 5156, 7010, 7457, 7834
Bsh1285I CGRYCG 1 cut(s) 2703
BshNI GGYRCC 1 cut(s) 4297
BsiEI CGRYCG 1 cut(s) 2703
BsiHKAI GWGCWC 4 cut(s) 746, 3522, 5402, 8538
BslFI GGGAC 9 cut(s) 3481, 3809, 4081, 5347, 6463, 6519, 6576, 6598, 8715
BsmFI GGGAC 9 cut(s) 3481, 3809, 4081, 5347, 6463, 6519, 6576, 6598, 8715
BsmI GAATGC 4 cut(s) 1116, 5946, 7631, 8290
Bso31I GGTCTC 1 cut(s) 8654
Bsp119I TTCGAA 2 cut(s) 3633, 8282
Bsp1286I GDGCHC 7 cut(s) 746, 2804, 3522, 3906, 5402, 6545, 8538
Bsp13I TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
Bsp1407I TGTACA 2 cut(s) 1416, 6130
Bsp19I CCATGG 2 cut(s) 1132, 6141
BspEI TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
BspFNI CGCG 5 cut(s) 1602, 5156, 7010, 7457, 7834
BspHI TCATGA 5 cut(s) 1171, 3975, 5356, 7159, 8062
BspLI GGNNCC 9 cut(s) 1702, 2878, 4299, 5832, 6586, 7570, 7692, 7928, 8703
BspMAI CTGCAG 1 cut(s) 6642
BspMI ACCTGC 5 cut(s) 1289, 2211, 2813, 3408, 4285
BspOI GCTAGC 2 cut(s) 1652, 2598
BspQI GCTCTTC 2 cut(s) 7047, 7950
BspT104I TTCGAA 2 cut(s) 3633, 8282
BspT107I GGYRCC 1 cut(s) 4297
BspTI CTTAAG 4 cut(s) 3556, 4483, 7058, 7961
BspTNI GGTCTC 1 cut(s) 8654
BsrFI RCCGGY 1 cut(s) 6015
BsrGI TGTACA 2 cut(s) 1416, 6130
BssAI RCCGGY 1 cut(s) 6015
BssNAI GTATAC 3 cut(s) 3293, 4694, 6013
BssSI CACGAG 2 cut(s) 2357, 3350
BssT1I CCWWGG 6 cut(s) 1093, 1132, 1372, 2479, 6141, 6723
Bst1107I GTATAC 3 cut(s) 3293, 4694, 6013
Bst2BI CACGAG 2 cut(s) 2357, 3350
Bst6I CTCTTC 7 cut(s) 306, 2805, 2925, 2933, 3951, 7047, 7950
BstAFI CTTAAG 4 cut(s) 3556, 4483, 7058, 7961
BstAPI GCANNNNNTGC 4 cut(s) 2252, 2725, 7637, 7646
BstAUI TGTACA 2 cut(s) 1416, 6130
BstBAI YACGTR 2 cut(s) 2507, 5567
BstBI TTCGAA 2 cut(s) 3633, 8282
BstDSI CCRYGG 5 cut(s) 1132, 1352, 2694, 5612, 6141
BstENI CCTNNNNNAGG 2 cut(s) 5763, 6690
BstFNI CGCG 5 cut(s) 1602, 5156, 7010, 7457, 7834
BstHHI GCGC 4 cut(s) 2162, 3552, 4346, 8258
BstMCI CGRYCG 1 cut(s) 2703
BstNSI RCATGY 9 cut(s) 725, 1548, 2393, 5289, 5689, 6949, 7396, 7719, 8337
BstPAI GACNNNNGTC 2 cut(s) 2366, 3816
BstSFI CTRYAG 5 cut(s) 94, 1021, 3920, 4074, 6638
BstSLI GKGCMC 2 cut(s) 3522, 5402
BstUI CGCG 5 cut(s) 1602, 5156, 7010, 7457, 7834
BstV2I GAAGAC 9 cut(s) 594, 1915, 2687, 4148, 4638, 5684, 5874, 5937, 8546
BstXI CCANNNNNNTGG 3 cut(s) 1622, 5669, 7687
BstZ17I GTATAC 3 cut(s) 3293, 4694, 6013
BsuI GTATCC 3 cut(s) 3288, 5157, 8446
BtgI CCRYGG 5 cut(s) 1132, 1352, 2694, 5612, 6141
BtgZI GCGATG 1 cut(s) 5178
BtrI CACGTC 1 cut(s) 2761
BtsI GCAGTG 5 cut(s) 1948, 5644, 6052, 6803, 8599
BveI ACCTGC 5 cut(s) 1289, 2211, 2813, 3408, 4285
CaiI CAGNNNCTG 1 cut(s) 3754
CciI TCATGA 5 cut(s) 1171, 3975, 5356, 7159, 8062
CfoI GCGC 4 cut(s) 2162, 3552, 4346, 8258
Cfr10I RCCGGY 1 cut(s) 6015
Cfr13I GGNCC 9 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8183, 8702
CseI GACGC 3 cut(s) 1591, 5993, 6790
CsiI ACCWGGT 1 cut(s) 2123
DraI TTTAAA 6 cut(s) 612, 2062, 2346, 3159, 3654, 4131
DraIII CACNNNGTG 1 cut(s) 2127
DrdI GACNNNNNNGTC 2 cut(s) 6475, 8614
DriI GACNNNNNGTC 1 cut(s) 6629
DseDI GACNNNNNNGTC 2 cut(s) 6475, 8614
EaeI YGGCCR 4 cut(s) 770, 3370, 7149, 8052
Eam1104I CTCTTC 7 cut(s) 306, 2805, 2925, 2933, 3951, 7047, 7950
Eam1105I GACNNNNNGTC 1 cut(s) 6629
EarI CTCTTC 7 cut(s) 306, 2805, 2925, 2933, 3951, 7047, 7950
Eco130I CCWWGG 6 cut(s) 1093, 1132, 1372, 2479, 6141, 6723
Eco24I GRGCYC 3 cut(s) 2804, 3906, 6545
Eco31I GGTCTC 1 cut(s) 8654
Eco32I GATATC 1 cut(s) 3647
Eco47I GGWCC 8 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8702
EcoNI CCTNNNNNAGG 2 cut(s) 5763, 6690
EcoRI GAATTC 4 cut(s) 733, 3791, 7660, 8399
EcoRV GATATC 1 cut(s) 3647
EcoT14I CCWWGG 6 cut(s) 1093, 1132, 1372, 2479, 6141, 6723
EcoT22I ATGCAT 3 cut(s) 1550, 2454, 7800
EcoT38I GRGCYC 3 cut(s) 2804, 3906, 6545
ErhI CCWWGG 6 cut(s) 1093, 1132, 1372, 2479, 6141, 6723
FaqI GGGAC 9 cut(s) 3481, 3809, 4081, 5347, 6463, 6519, 6576, 6598, 8715
FauI CCCGC 1 cut(s) 8208
FbaI TGATCA 8 cut(s) 712, 753, 1363, 1966, 5404, 6226, 8320, 8515
FblI GTMKAC 5 cut(s) 3292, 3774, 4178, 4693, 6012
FriOI GRGCYC 3 cut(s) 2804, 3906, 6545
FspAI RTGCGCAY 1 cut(s) 8257
FspI TGCGCA 2 cut(s) 3551, 8257
GlaI GCGC 4 cut(s) 2161, 3551, 4345, 8257
GsaI CCCAGC 1 cut(s) 6722
HgaI GACGC 3 cut(s) 1591, 5993, 6790
HhaI GCGC 4 cut(s) 2162, 3552, 4346, 8258
Hin6I GCGC 4 cut(s) 2160, 3550, 4344, 8256
HinP1I GCGC 4 cut(s) 2160, 3550, 4344, 8256
HincII GTYRAC 1 cut(s) 5797
HindII GTYRAC 1 cut(s) 5797
HindIII AAGCTT 6 cut(s) 114, 2307, 2900, 3625, 5952, 6967
HpaI GTTAAC 1 cut(s) 5797
Hpy99I CGWCG 1 cut(s) 7268
HspAI GCGC 4 cut(s) 2160, 3550, 4344, 8256
Kpn2I TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
KpnI GGTACC 1 cut(s) 4301
Ksp22I TGATCA 8 cut(s) 712, 753, 1363, 1966, 5404, 6226, 8320, 8515
KspAI GTTAAC 1 cut(s) 5797
LguI GCTCTTC 2 cut(s) 7047, 7950
MabI ACCWGGT 1 cut(s) 2123
MfeI CAATTG 4 cut(s) 2067, 3885, 7665, 7884
MhlI GDGCHC 7 cut(s) 746, 2804, 3522, 3906, 5402, 6545, 8538
MlsI TGGCCA 1 cut(s) 3372
MluNI TGGCCA 1 cut(s) 3372
MlyI GAGTC 5 cut(s) 2308, 2924, 4162, 5329, 6463
Mox20I TGGCCA 1 cut(s) 3372
Mph1103I ATGCAT 3 cut(s) 1550, 2454, 7800
MroI TCCGGA 4 cut(s) 1408, 4018, 4159, 6436
MroXI GAANNNNTTC 6 cut(s) 116, 2574, 4635, 4737, 6602, 7785
MscI TGGCCA 1 cut(s) 3372
MslI CAYNNNNRTG 7 cut(s) 720, 1081, 4062, 5049, 6675, 8178, 8628
Msp20I TGGCCA 1 cut(s) 3372
MspA1I CMGCKG 2 cut(s) 4823, 7855
MspCI CTTAAG 4 cut(s) 3556, 4483, 7058, 7961
MunI CAATTG 4 cut(s) 2067, 3885, 7665, 7884
Mva1269I GAATGC 4 cut(s) 1116, 5946, 7631, 8290
MvnI CGCG 5 cut(s) 1602, 5156, 7010, 7457, 7834
NciI CCSGG 3 cut(s) 1209, 5862, 6651
NcoI CCATGG 2 cut(s) 1132, 6141
NheI GCTAGC 2 cut(s) 1648, 2594
NlaIV GGNNCC 9 cut(s) 1702, 2878, 4299, 5832, 6586, 7570, 7692, 7928, 8703
NmeAIII GCCGAG 1 cut(s) 5288
NsbI TGCGCA 2 cut(s) 3551, 8257
NsiI ATGCAT 3 cut(s) 1550, 2454, 7800
NspI RCATGY 9 cut(s) 725, 1548, 2393, 5289, 5689, 6949, 7396, 7719, 8337
NspV TTCGAA 2 cut(s) 3633, 8282
OliI CACNNNNGTG 1 cut(s) 1081
PaeI GCATGC 1 cut(s) 725
PagI TCATGA 5 cut(s) 1171, 3975, 5356, 7159, 8062
PciI ACATGT 3 cut(s) 2389, 7715, 8333
PciSI GCTCTTC 2 cut(s) 7047, 7950
PcsI WCGNNNNNNNCGW 1 cut(s) 5160
PctI GAATGC 4 cut(s) 1116, 5946, 7631, 8290
PdmI GAANNNNTTC 6 cut(s) 116, 2574, 4635, 4737, 6602, 7785
PflMI CCANNNNNTGG 2 cut(s) 377, 3887
PfoI TCCNGGA 4 cut(s) 1207, 4725, 7485, 7693
Ple19I CGATCG 1 cut(s) 2703
PleI GAGTC 5 cut(s) 2308, 2923, 4161, 5329, 6463
PpsI GAGTC 5 cut(s) 2308, 2923, 4161, 5329, 6463
Ppu21I YACGTR 2 cut(s) 2507, 5567
PscI ACATGT 3 cut(s) 2389, 7715, 8333
PshAI GACNNNNGTC 2 cut(s) 2366, 3816
PshBI ATTAAT 1 cut(s) 4493
PsiI TTATAA 1 cut(s) 4784
PspFI CCCAGC 1 cut(s) 6718
PspN4I GGNNCC 9 cut(s) 1702, 2878, 4299, 5832, 6586, 7570, 7692, 7928, 8703
PspPI GGNCC 9 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8183, 8702
PstI CTGCAG 1 cut(s) 6642
PstNI CAGNNNCTG 1 cut(s) 3754
PvuI CGATCG 1 cut(s) 2703
PvuII CAGCTG 1 cut(s) 7855
RseI CAYNNNNRTG 7 cut(s) 720, 1081, 4062, 5049, 6675, 8178, 8628
SapI GCTCTTC 2 cut(s) 7047, 7950
Sau96I GGNCC 9 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8183, 8702
ScaI AGTACT 4 cut(s) 1389, 1741, 4948, 8677
SchI GAGTC 5 cut(s) 2308, 2924, 4162, 5329, 6463
SduI GDGCHC 7 cut(s) 746, 2804, 3522, 3906, 5402, 6545, 8538
SexAI ACCWGGT 1 cut(s) 2123
SfcI CTRYAG 5 cut(s) 94, 1021, 3920, 4074, 6638
SfuI TTCGAA 2 cut(s) 3633, 8282
SinI GGWCC 8 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8702
SmiMI CAYNNNNRTG 7 cut(s) 720, 1081, 4062, 5049, 6675, 8178, 8628
SpeI ACTAGT 3 cut(s) 1736, 7136, 8039
SphI GCATGC 1 cut(s) 725
SspI AATATT 7 cut(s) 1523, 2440, 2575, 3147, 3154, 5084, 5547
StyI CCWWGG 6 cut(s) 1093, 1132, 1372, 2479, 6141, 6723
TaqII GACCGA 3 cut(s) 6349, 7512, 8678
TatI WGTACW 9 cut(s) 524, 1387, 1416, 1739, 4946, 5304, 6130, 6642, 8675
TauI GCSGC 1 cut(s) 1734
TspGWI ACGGA 4 cut(s) 1693, 3374, 5782, 8378
Van91I CCANNNNNTGG 2 cut(s) 377, 3887
Vha464I CTTAAG 4 cut(s) 3556, 4483, 7058, 7961
VneI GTGCAC 2 cut(s) 3518, 5398
VpaK11BI GGWCC 8 cut(s) 1701, 2785, 2877, 3134, 5831, 6585, 7568, 8702
VspI ATTAAT 1 cut(s) 4493
XagI CCTNNNNNAGG 2 cut(s) 5763, 6690
XbaI TCTAGA 1 cut(s) 7604
XceI RCATGY 9 cut(s) 725, 1548, 2393, 5289, 5689, 6949, 7396, 7719, 8337
XcmI CCANNNNNNNNNTGG 8 cut(s) 137, 735, 1807, 2128, 3961, 5824, 7127, 8030
XmaJI CCTAGG 1 cut(s) 2479
XmiI GTMKAC 5 cut(s) 3292, 3774, 4178, 4693, 6012
XmnI GAANNNNTTC 6 cut(s) 116, 2574, 4635, 4737, 6602, 7785
ZrmI AGTACT 4 cut(s) 1389, 1741, 4948, 8677
Zsp2I ATGCAT 3 cut(s) 1550, 2454, 7800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.