Belongs to the glycosyltransferase 2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 968 |
| AccII | CGCG | 1 cut(s) | 1154 |
| AciI | CCGC | 3 cut(s) | 383, 1152, 1193 |
| AclWI | GGATC | 3 cut(s) | 116, 493, 583 |
| AcoI | YGGCCR | 4 cut(s) | 164, 1149, 1241, 1290 |
| AcsI | RAATTY | 5 cut(s) | 159, 304, 336, 624, 1033 |
| AcyI | GRCGYC | 1 cut(s) | 606 |
| AfaI | GTAC | 1 cut(s) | 1104 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 702, 808, 1158, 1178 |
| AgsI | TTSAA | 4 cut(s) | 61, 115, 185, 1013 |
| AjnI | CCWGG | 4 cut(s) | 166, 544, 801, 1198 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 770, 802 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 1340 |
| AluBI | AGCT | 9 cut(s) | 176, 316, 329, 673, 761, 985, 1058, 1139, 1310 |
| AluI | AGCT | 9 cut(s) | 176, 316, 329, 673, 761, 985, 1058, 1139, 1310 |
| Alw21I | GWGCWC | 1 cut(s) | 318 |
| Alw26I | GTCTC | 2 cut(s) | 274, 1095 |
| AlwI | GGATC | 3 cut(s) | 116, 493, 583 |
| AoxI | GGCC | 7 cut(s) | 164, 693, 1149, 1195, 1241, 1290, 1317 |
| ApaI | GGGCCC | 1 cut(s) | 1199 |
| ApeKI | GCWGC | 3 cut(s) | 392, 616, 827 |
| ApoI | RAATTY | 5 cut(s) | 159, 304, 336, 624, 1033 |
| AspS9I | GGNCC | 5 cut(s) | 188, 799, 920, 1195, 1196 |
| AsuHPI | GGTGA | 2 cut(s) | 1015, 1195 |
| AvaII | GGWCC | 3 cut(s) | 188, 799, 920 |
| BaeGI | GKGCMC | 2 cut(s) | 560, 1199 |
| BalI | TGGCCA | 3 cut(s) | 166, 1243, 1292 |
| BanII | GRGCYC | 2 cut(s) | 318, 1199 |
| Bbv12I | GWGCWC | 1 cut(s) | 318 |
| BbvI | GCAGC | 3 cut(s) | 404, 603, 814 |
| BceAI | ACGGC | 1 cut(s) | 1164 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 124, 158 |
| BciT130I | CCWGG | 4 cut(s) | 168, 546, 803, 1200 |
| BclI | TGATCA | 1 cut(s) | 358 |
| BcoDI | GTCTC | 2 cut(s) | 274, 1095 |
| BfaI | CTAG | 3 cut(s) | 620, 1044, 1071 |
| BfmI | CTRYAG | 1 cut(s) | 1296 |
| BisI | GCNGC | 4 cut(s) | 393, 617, 828, 1152 |
| BlsI | GCNGC | 4 cut(s) | 394, 618, 829, 1153 |
| Bme1390I | CCNGG | 4 cut(s) | 168, 546, 803, 1200 |
| Bme18I | GGWCC | 3 cut(s) | 188, 799, 920 |
| BmgT120I | GGNCC | 5 cut(s) | 188, 799, 920, 1195, 1196 |
| BmiI | GGNNCC | 4 cut(s) | 807, 905, 921, 1197 |
| BmrFI | CCNGG | 4 cut(s) | 168, 546, 803, 1200 |
| BmrI | ACTGGG | 1 cut(s) | 498 |
| BmsI | GCATC | 4 cut(s) | 379, 696, 959, 1057 |
| BmuI | ACTGGG | 1 cut(s) | 498 |
| BpmI | CTGGAG | 1 cut(s) | 294 |
| BpuEI | CTTGAG | 1 cut(s) | 695 |
| BsaBI | GATNNNNATC | 1 cut(s) | 497 |
| BsaHI | GRCGYC | 1 cut(s) | 606 |
| BsaJI | CCNNGG | 5 cut(s) | 167, 923, 1198, 1199, 1287 |
| BsaWI | WCCGGW | 1 cut(s) | 364 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1196, 1226 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 702, 808, 1158, 1178 |
| Bse118I | RCCGGY | 2 cut(s) | 808, 1171 |
| Bse1I | ACTGG | 1 cut(s) | 493 |
| Bse3DI | GCAATG | 1 cut(s) | 692 |
| Bse8I | GATNNNNATC | 1 cut(s) | 497 |
| BseBI | CCWGG | 4 cut(s) | 168, 546, 803, 1200 |
| BseDI | CCNNGG | 5 cut(s) | 167, 923, 1198, 1199, 1287 |
| BseGI | GGATG | 5 cut(s) | 215, 318, 498, 711, 1355 |
| BseJI | GATNNNNATC | 1 cut(s) | 497 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 702, 808, 1158, 1178 |
| BseMI | GCAATG | 1 cut(s) | 692 |
| BseMII | CTCAG | 2 cut(s) | 952, 990 |
| BseNI | ACTGG | 1 cut(s) | 493 |
| BseSI | GKGCMC | 2 cut(s) | 560, 1199 |
| BseX3I | CGGCCG | 1 cut(s) | 1149 |
| BseXI | GCAGC | 3 cut(s) | 404, 603, 814 |
| BseYI | CCCAGC | 1 cut(s) | 176 |
| BsgI | GTGCAG | 3 cut(s) | 546, 707, 1112 |
| Bsh1236I | CGCG | 1 cut(s) | 1154 |
| Bsh1285I | CGRYCG | 2 cut(s) | 387, 1152 |
| BshFI | GGCC | 7 cut(s) | 166, 695, 1151, 1197, 1243, 1292, 1319 |
| BsiEI | CGRYCG | 2 cut(s) | 387, 1152 |
| BsiHKAI | GWGCWC | 1 cut(s) | 318 |
| BsiSI | CCGG | 3 cut(s) | 365, 809, 1172 |
| BslFI | GGGAC | 4 cut(s) | 217, 630, 732, 933 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 702, 808, 1158, 1178 |
| BsmAI | GTCTC | 2 cut(s) | 274, 1095 |
| BsmFI | GGGAC | 4 cut(s) | 217, 630, 732, 933 |
| BsmI | GAATGC | 1 cut(s) | 299 |
| BsnI | GGCC | 7 cut(s) | 166, 695, 1151, 1197, 1243, 1292, 1319 |
| Bsp120I | GGGCCC | 1 cut(s) | 1195 |
| Bsp1286I | GDGCHC | 3 cut(s) | 318, 560, 1199 |
| Bsp143I | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| Bsp19I | CCATGG | 2 cut(s) | 923, 1287 |
| BspACI | CCGC | 3 cut(s) | 383, 1152, 1193 |
| BspANI | GGCC | 7 cut(s) | 166, 695, 1151, 1197, 1243, 1292, 1319 |
| BspCNI | CTCAG | 2 cut(s) | 951, 991 |
| BspFNI | CGCG | 1 cut(s) | 1154 |
| BspLI | GGNNCC | 4 cut(s) | 807, 905, 921, 1197 |
| BspPI | GGATC | 3 cut(s) | 116, 493, 583 |
| BspQI | GCTCTTC | 2 cut(s) | 756, 1331 |
| BsrDI | GCAATG | 1 cut(s) | 692 |
| BsrFI | RCCGGY | 2 cut(s) | 808, 1171 |
| BsrI | ACTGG | 1 cut(s) | 493 |
| BssAI | RCCGGY | 2 cut(s) | 808, 1171 |
| BssECI | CCNNGG | 5 cut(s) | 167, 923, 1198, 1199, 1287 |
| BssMI | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| BssNI | GRCGYC | 1 cut(s) | 606 |
| BssT1I | CCWWGG | 2 cut(s) | 923, 1287 |
| Bst2UI | CCWGG | 4 cut(s) | 168, 546, 803, 1200 |
| Bst4CI | ACNGT | 3 cut(s) | 239, 916, 973 |
| Bst6I | CTCTTC | 4 cut(s) | 593, 756, 1045, 1331 |
| BstACI | GRCGYC | 1 cut(s) | 606 |
| BstC8I | GCNNGC | 3 cut(s) | 533, 759, 1195 |
| BstDEI | CTNAG | 3 cut(s) | 938, 999, 1344 |
| BstDSI | CCRYGG | 2 cut(s) | 923, 1287 |
| BstF5I | GGATG | 5 cut(s) | 215, 318, 498, 711, 1355 |
| BstFNI | CGCG | 1 cut(s) | 1154 |
| BstKTI | GATC | 8 cut(s) | 20, 124, 326, 361, 501, 591, 739, 887 |
| BstMAI | GTCTC | 2 cut(s) | 274, 1095 |
| BstMBI | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| BstMCI | CGRYCG | 2 cut(s) | 387, 1152 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 389, 1316, 1330 |
| BstNI | CCWGG | 4 cut(s) | 168, 546, 803, 1200 |
| BstNSI | RCATGY | 1 cut(s) | 535 |
| BstSCI | CCNGG | 4 cut(s) | 166, 544, 801, 1198 |
| BstSFI | CTRYAG | 1 cut(s) | 1296 |
| BstSLI | GKGCMC | 2 cut(s) | 560, 1199 |
| BstUI | CGCG | 1 cut(s) | 1154 |
| BstV1I | GCAGC | 3 cut(s) | 404, 603, 814 |
| BstX2I | RGATCY | 1 cut(s) | 498 |
| BstYI | RGATCY | 1 cut(s) | 498 |
| BstZI | CGGCCG | 1 cut(s) | 1149 |
| BsuRI | GGCC | 7 cut(s) | 166, 695, 1151, 1197, 1243, 1292, 1319 |
| BtgI | CCRYGG | 2 cut(s) | 923, 1287 |
| BtgZI | GCGATG | 1 cut(s) | 53 |
| BtsCI | GGATG | 5 cut(s) | 215, 318, 498, 711, 1355 |
| BtsI | GCAGTG | 1 cut(s) | 558 |
| BtsIMutI | CAGTG | 2 cut(s) | 558, 921 |
| Cac8I | GCNNGC | 3 cut(s) | 533, 759, 1195 |
| Cfr10I | RCCGGY | 2 cut(s) | 808, 1171 |
| Cfr13I | GGNCC | 5 cut(s) | 188, 799, 920, 1195, 1196 |
| CseI | GACGC | 1 cut(s) | 595 |
| CsiI | ACCWGGT | 1 cut(s) | 544 |
| Csp6I | GTAC | 1 cut(s) | 1103 |
| CviQI | GTAC | 1 cut(s) | 1103 |
| DdeI | CTNAG | 3 cut(s) | 938, 999, 1344 |
| DpnI | GATC | 8 cut(s) | 19, 123, 325, 360, 500, 590, 738, 886 |
| DpnII | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| EaeI | YGGCCR | 4 cut(s) | 164, 1149, 1241, 1290 |
| EagI | CGGCCG | 1 cut(s) | 1149 |
| Eam1104I | CTCTTC | 4 cut(s) | 593, 756, 1045, 1331 |
| EarI | CTCTTC | 4 cut(s) | 593, 756, 1045, 1331 |
| Ecl136II | GAGCTC | 1 cut(s) | 316 |
| EclXI | CGGCCG | 1 cut(s) | 1149 |
| Eco130I | CCWWGG | 2 cut(s) | 923, 1287 |
| Eco24I | GRGCYC | 2 cut(s) | 318, 1199 |
| Eco32I | GATATC | 1 cut(s) | 481 |
| Eco47I | GGWCC | 3 cut(s) | 188, 799, 920 |
| Eco52I | CGGCCG | 1 cut(s) | 1149 |
| Eco53kI | GAGCTC | 1 cut(s) | 316 |
| EcoICRI | GAGCTC | 1 cut(s) | 316 |
| EcoO109I | RGGNCCY | 1 cut(s) | 188 |
| EcoRII | CCWGG | 4 cut(s) | 166, 544, 801, 1198 |
| EcoRV | GATATC | 1 cut(s) | 481 |
| EcoT14I | CCWWGG | 2 cut(s) | 923, 1287 |
| EcoT22I | ATGCAT | 1 cut(s) | 533 |
| EcoT38I | GRGCYC | 2 cut(s) | 318, 1199 |
| ErhI | CCWWGG | 2 cut(s) | 923, 1287 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 509, 541 |
| FaqI | GGGAC | 4 cut(s) | 217, 630, 732, 933 |
| FauI | CCCGC | 1 cut(s) | 1186 |
| FbaI | TGATCA | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 4 cut(s) | 393, 617, 828, 1152 |
| FokI | GGATG | 5 cut(s) | 222, 305, 505, 718, 1342 |
| FriOI | GRGCYC | 2 cut(s) | 318, 1199 |
| Fsp4HI | GCNGC | 4 cut(s) | 393, 617, 828, 1152 |
| FspBI | CTAG | 3 cut(s) | 620, 1044, 1071 |
| GluI | GCNGC | 4 cut(s) | 393, 617, 828, 1152 |
| GsaI | CCCAGC | 1 cut(s) | 180 |
| GsuI | CTGGAG | 1 cut(s) | 294 |
| HaeIII | GGCC | 7 cut(s) | 166, 695, 1151, 1197, 1243, 1292, 1319 |
| HapII | CCGG | 3 cut(s) | 365, 809, 1172 |
| HgaI | GACGC | 1 cut(s) | 595 |
| Hin1I | GRCGYC | 1 cut(s) | 606 |
| HindIII | AAGCTT | 2 cut(s) | 671, 983 |
| HinfI | GANTC | 4 cut(s) | 181, 875, 1088, 1282 |
| HpaII | CCGG | 3 cut(s) | 365, 809, 1172 |
| HphI | GGTGA | 2 cut(s) | 1015, 1195 |
| Hpy166II | GTNNAC | 1 cut(s) | 1077 |
| Hpy188I | TCNGA | 3 cut(s) | 323, 415, 1000 |
| Hpy188III | TCNNGA | 4 cut(s) | 311, 592, 734, 1359 |
| Hpy8I | GTNNAC | 1 cut(s) | 1077 |
| HpyAV | CCTTC | 1 cut(s) | 254 |
| HpyCH4III | ACNGT | 3 cut(s) | 239, 916, 973 |
| HpyCH4IV | ACGT | 1 cut(s) | 223 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 389, 1316, 1330 |
| HpyF3I | CTNAG | 3 cut(s) | 938, 999, 1344 |
| HpySE526I | ACGT | 1 cut(s) | 223 |
| Hsp92I | GRCGYC | 1 cut(s) | 606 |
| Ksp22I | TGATCA | 1 cut(s) | 358 |
| Kzo9I | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| LguI | GCTCTTC | 2 cut(s) | 756, 1331 |
| LmnI | GCTCC | 2 cut(s) | 927, 1027 |
| Lsp1109I | GCAGC | 3 cut(s) | 404, 603, 814 |
| LweI | GCATC | 4 cut(s) | 379, 696, 959, 1057 |
| MabI | ACCWGGT | 1 cut(s) | 544 |
| MaeI | CTAG | 3 cut(s) | 620, 1044, 1071 |
| MaeII | ACGT | 1 cut(s) | 223 |
| MaeIII | GTNAC | 6 cut(s) | 469, 607, 1003, 1015, 1046, 1144 |
| MalI | GATC | 8 cut(s) | 19, 123, 325, 360, 500, 590, 738, 886 |
| MboI | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| MboII | GAAGA | 8 cut(s) | 16, 23, 488, 508, 610, 743, 1032, 1318 |
| MfeI | CAATTG | 1 cut(s) | 908 |
| MflI | RGATCY | 1 cut(s) | 498 |
| MhlI | GDGCHC | 3 cut(s) | 318, 560, 1199 |
| MlsI | TGGCCA | 3 cut(s) | 166, 1243, 1292 |
| MluCI | AATT | 9 cut(s) | 159, 304, 336, 624, 640, 711, 888, 908, 1033 |
| MluNI | TGGCCA | 3 cut(s) | 166, 1243, 1292 |
| MlyI | GAGTC | 1 cut(s) | 1097 |
| MmeI | TCCRAC | 1 cut(s) | 891 |
| Mox20I | TGGCCA | 3 cut(s) | 166, 1243, 1292 |
| Mph1103I | ATGCAT | 1 cut(s) | 533 |
| MscI | TGGCCA | 3 cut(s) | 166, 1243, 1292 |
| MseI | TTAA | 5 cut(s) | 843, 851, 944, 981, 1251 |
| Msp20I | TGGCCA | 3 cut(s) | 166, 1243, 1292 |
| MspA1I | CMGCKG | 1 cut(s) | 1310 |
| MspI | CCGG | 3 cut(s) | 365, 809, 1172 |
| MspR9I | CCNGG | 4 cut(s) | 168, 546, 803, 1200 |
| MunI | CAATTG | 1 cut(s) | 908 |
| Mva1269I | GAATGC | 1 cut(s) | 299 |
| MvaI | CCWGG | 4 cut(s) | 168, 546, 803, 1200 |
| MvnI | CGCG | 1 cut(s) | 1154 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 389, 1316, 1330 |
| NcoI | CCATGG | 2 cut(s) | 923, 1287 |
| NdeII | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| NlaIV | GGNNCC | 4 cut(s) | 807, 905, 921, 1197 |
| NmuCI | GTSAC | 5 cut(s) | 607, 1003, 1015, 1046, 1144 |
| NsiI | ATGCAT | 1 cut(s) | 533 |
| NspI | RCATGY | 1 cut(s) | 535 |
| PaeI | GCATGC | 1 cut(s) | 535 |
| PasI | CCCWGGG | 1 cut(s) | 1199 |
| PciSI | GCTCTTC | 2 cut(s) | 756, 1331 |
| PctI | GAATGC | 1 cut(s) | 299 |
| PfeI | GAWTC | 3 cut(s) | 181, 875, 1282 |
| PfoI | TCCNGGA | 1 cut(s) | 801 |
| PkrI | GCNGC | 4 cut(s) | 394, 618, 829, 1153 |
| PleI | GAGTC | 1 cut(s) | 1096 |
| PpsI | GAGTC | 1 cut(s) | 1096 |
| PpuMI | RGGWCCY | 1 cut(s) | 188 |
| PsiI | TTATAA | 1 cut(s) | 968 |
| Psp124BI | GAGCTC | 1 cut(s) | 318 |
| Psp5II | RGGWCCY | 1 cut(s) | 188 |
| Psp6I | CCWGG | 4 cut(s) | 166, 544, 801, 1198 |
| PspFI | CCCAGC | 1 cut(s) | 176 |
| PspGI | CCWGG | 4 cut(s) | 166, 544, 801, 1198 |
| PspN4I | GGNNCC | 4 cut(s) | 807, 905, 921, 1197 |
| PspOMI | GGGCCC | 1 cut(s) | 1195 |
| PspPI | GGNCC | 5 cut(s) | 188, 799, 920, 1195, 1196 |
| PspPPI | RGGWCCY | 1 cut(s) | 188 |
| PsuI | RGATCY | 1 cut(s) | 498 |
| PvuII | CAGCTG | 1 cut(s) | 1310 |
| RsaI | GTAC | 1 cut(s) | 1104 |
| RsaNI | GTAC | 1 cut(s) | 1103 |
| SacI | GAGCTC | 1 cut(s) | 318 |
| SapI | GCTCTTC | 2 cut(s) | 756, 1331 |
| SaqAI | TTAA | 5 cut(s) | 843, 851, 944, 981, 1251 |
| SatI | GCNGC | 4 cut(s) | 393, 617, 828, 1152 |
| Sau3AI | GATC | 8 cut(s) | 17, 121, 323, 358, 498, 588, 736, 884 |
| Sau96I | GGNCC | 5 cut(s) | 188, 799, 920, 1195, 1196 |
| SchI | GAGTC | 1 cut(s) | 1097 |
| ScrFI | CCNGG | 4 cut(s) | 168, 546, 803, 1200 |
| SduI | GDGCHC | 3 cut(s) | 318, 560, 1199 |
| SexAI | ACCWGGT | 1 cut(s) | 544 |
| SfaNI | GCATC | 4 cut(s) | 379, 696, 959, 1057 |
| SfcI | CTRYAG | 1 cut(s) | 1296 |
| SinI | GGWCC | 3 cut(s) | 188, 799, 920 |
| SmlI | CTYRAG | 1 cut(s) | 674 |
| SmoI | CTYRAG | 1 cut(s) | 674 |
| SphI | GCATGC | 1 cut(s) | 535 |
| Sse9I | AATT | 9 cut(s) | 159, 304, 336, 624, 640, 711, 888, 908, 1033 |
| SsiI | CCGC | 3 cut(s) | 383, 1152, 1193 |
| SspI | AATATT | 3 cut(s) | 355, 822, 1098 |
| SspMI | CTAG | 3 cut(s) | 620, 1044, 1071 |
| SstI | GAGCTC | 1 cut(s) | 318 |
| StyD4I | CCNGG | 4 cut(s) | 166, 544, 801, 1198 |
| StyI | CCWWGG | 2 cut(s) | 923, 1287 |
| TaaI | ACNGT | 3 cut(s) | 239, 916, 973 |
| TaiI | ACGT | 1 cut(s) | 226 |
| TaqI | TCGA | 4 cut(s) | 144, 387, 420, 883 |
| TasI | AATT | 9 cut(s) | 159, 304, 336, 624, 640, 711, 888, 908, 1033 |
| TauI | GCSGC | 1 cut(s) | 1154 |
| TfiI | GAWTC | 3 cut(s) | 181, 875, 1282 |
| Tru1I | TTAA | 5 cut(s) | 843, 851, 944, 981, 1251 |
| Tru9I | TTAA | 5 cut(s) | 843, 851, 944, 981, 1251 |
| TscAI | CASTG | 2 cut(s) | 565, 921 |
| TseFI | GTSAC | 5 cut(s) | 607, 1003, 1015, 1046, 1144 |
| TseI | GCWGC | 3 cut(s) | 392, 616, 827 |
| Tsp45I | GTSAC | 5 cut(s) | 607, 1003, 1015, 1046, 1144 |
| TspDTI | ATGAA | 6 cut(s) | 23, 81, 231, 273, 509, 1318 |
| TspRI | CASTG | 2 cut(s) | 565, 921 |
| VpaK11BI | GGWCC | 3 cut(s) | 188, 799, 920 |
| XapI | RAATTY | 5 cut(s) | 159, 304, 336, 624, 1033 |
| XceI | RCATGY | 1 cut(s) | 535 |
| XspI | CTAG | 3 cut(s) | 620, 1044, 1071 |
| Zsp2I | ATGCAT | 1 cut(s) | 533 |