Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 157 - 459 | 1.6e-57 | Phosphofructokinase |
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1621 |
| AccB1I | GGYRCC | 1 cut(s) | 656 |
| AccI | GTMKAC | 2 cut(s) | 152, 1029 |
| AccIII | TCCGGA | 1 cut(s) | 1193 |
| AclWI | GGATC | 3 cut(s) | 604, 1254, 1289 |
| AcoI | YGGCCR | 3 cut(s) | 169, 219, 682 |
| AcsI | RAATTY | 2 cut(s) | 561, 828 |
| AcuI | CTGAAG | 2 cut(s) | 1127, 1602 |
| AfaI | GTAC | 2 cut(s) | 719, 744 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 666, 1421 |
| AflIII | ACRYGT | 2 cut(s) | 479, 543 |
| AgsI | TTSAA | 3 cut(s) | 12, 932, 1097 |
| AjiI | CACGTC | 1 cut(s) | 431 |
| AjnI | CCWGG | 2 cut(s) | 496, 1072 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 158, 190 |
| AluBI | AGCT | 6 cut(s) | 75, 773, 942, 1159, 1204, 1530 |
| AluI | AGCT | 6 cut(s) | 75, 773, 942, 1159, 1204, 1530 |
| Alw26I | GTCTC | 3 cut(s) | 47, 63, 1017 |
| AlwI | GGATC | 3 cut(s) | 604, 1254, 1289 |
| Ama87I | CYCGRG | 1 cut(s) | 60 |
| Aor13HI | TCCGGA | 1 cut(s) | 1193 |
| AoxI | GGCC | 7 cut(s) | 169, 219, 226, 682, 805, 976, 1411 |
| ApeKI | GCWGC | 3 cut(s) | 80, 105, 920 |
| ApoI | RAATTY | 2 cut(s) | 561, 828 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 379, 411, 857, 889, 1204, 1236 |
| AseI | ATTAAT | 2 cut(s) | 729, 915 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 252 |
| AspS9I | GGNCC | 6 cut(s) | 227, 395, 425, 976, 1070, 1412 |
| AsuC2I | CCSGG | 1 cut(s) | 857 |
| AsuHPI | GGTGA | 1 cut(s) | 1465 |
| AsuNHI | GCTAGC | 1 cut(s) | 1016 |
| AvaI | CYCGRG | 1 cut(s) | 60 |
| AvaII | GGWCC | 3 cut(s) | 395, 425, 1070 |
| BaeGI | GKGCMC | 1 cut(s) | 1501 |
| BalI | TGGCCA | 1 cut(s) | 221 |
| BanI | GGYRCC | 1 cut(s) | 656 |
| BanII | GRGCYC | 1 cut(s) | 213 |
| BauI | CACGAG | 1 cut(s) | 675 |
| BbvI | GCAGC | 3 cut(s) | 67, 92, 932 |
| BccI | CCATC | 5 cut(s) | 386, 668, 674, 749, 1378 |
| BceAI | ACGGC | 4 cut(s) | 36, 133, 156, 184 |
| BciT130I | CCWGG | 2 cut(s) | 498, 1074 |
| BciVI | GTATCC | 3 cut(s) | 303, 575, 1385 |
| BclI | TGATCA | 1 cut(s) | 1479 |
| BcnI | CCSGG | 1 cut(s) | 857 |
| BcoDI | GTCTC | 3 cut(s) | 47, 63, 1017 |
| BfaI | CTAG | 2 cut(s) | 1017, 1626 |
| BfmI | CTRYAG | 1 cut(s) | 982 |
| BfuI | GTATCC | 3 cut(s) | 303, 575, 1385 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 234 |
| BglII | AGATCT | 1 cut(s) | 869 |
| BisI | GCNGC | 6 cut(s) | 81, 106, 235, 685, 921, 979 |
| BlpI | GCTNAGC | 1 cut(s) | 903 |
| BlsI | GCNGC | 6 cut(s) | 82, 107, 236, 686, 922, 980 |
| Bme1390I | CCNGG | 3 cut(s) | 498, 857, 1074 |
| Bme18I | GGWCC | 3 cut(s) | 395, 425, 1070 |
| BmeT110I | CYCGRG | 1 cut(s) | 60 |
| BmgBI | CACGTC | 1 cut(s) | 431 |
| BmgT120I | GGNCC | 6 cut(s) | 227, 395, 425, 976, 1070, 1412 |
| BmiI | GGNNCC | 2 cut(s) | 229, 658 |
| BmrFI | CCNGG | 3 cut(s) | 498, 857, 1074 |
| BmrI | ACTGGG | 1 cut(s) | 90 |
| BmsI | GCATC | 3 cut(s) | 720, 1177, 1340 |
| BmtI | GCTAGC | 1 cut(s) | 1020 |
| BmuI | ACTGGG | 1 cut(s) | 90 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1035 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 903 |
| BpuMI | CCSGG | 1 cut(s) | 857 |
| BsaJI | CCNNGG | 2 cut(s) | 398, 664 |
| BsaWI | WCCGGW | 1 cut(s) | 1193 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 302, 332 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 666, 1421 |
| Bse118I | RCCGGY | 1 cut(s) | 224 |
| Bse1I | ACTGG | 5 cut(s) | 90, 96, 177, 630, 715 |
| Bse3DI | GCAATG | 2 cut(s) | 1005, 1326 |
| BseAI | TCCGGA | 1 cut(s) | 1193 |
| BseBI | CCWGG | 2 cut(s) | 498, 1074 |
| BseDI | CCNNGG | 2 cut(s) | 398, 664 |
| BseGI | GGATG | 8 cut(s) | 273, 361, 397, 639, 660, 711, 852, 1192 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 666, 1421 |
| BseMI | GCAATG | 2 cut(s) | 1005, 1326 |
| BseMII | CTCAG | 1 cut(s) | 917 |
| BseNI | ACTGG | 5 cut(s) | 90, 96, 177, 630, 715 |
| BseRI | GAGGAG | 2 cut(s) | 765, 911 |
| BseSI | GKGCMC | 1 cut(s) | 1501 |
| BseX3I | CGGCCG | 1 cut(s) | 169 |
| BseXI | GCAGC | 3 cut(s) | 67, 92, 932 |
| BsgI | GTGCAG | 1 cut(s) | 1167 |
| Bsh1285I | CGRYCG | 1 cut(s) | 172 |
| BshFI | GGCC | 7 cut(s) | 171, 221, 228, 684, 807, 978, 1413 |
| BshNI | GGYRCC | 1 cut(s) | 656 |
| BsiEI | CGRYCG | 1 cut(s) | 172 |
| BsiHKCI | CYCGRG | 1 cut(s) | 60 |
| BsiSI | CCGG | 3 cut(s) | 225, 857, 1194 |
| BslFI | GGGAC | 3 cut(s) | 78, 418, 682 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 666, 1421 |
| BsmAI | GTCTC | 3 cut(s) | 47, 63, 1017 |
| BsmBI | CGTCTC | 1 cut(s) | 1017 |
| BsmFI | GGGAC | 3 cut(s) | 78, 418, 682 |
| BsnI | GGCC | 7 cut(s) | 171, 221, 228, 684, 807, 978, 1413 |
| BsoBI | CYCGRG | 1 cut(s) | 60 |
| Bsp1286I | GDGCHC | 2 cut(s) | 213, 1501 |
| Bsp13I | TCCGGA | 1 cut(s) | 1193 |
| Bsp143I | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 903 |
| BspANI | GGCC | 7 cut(s) | 171, 221, 228, 684, 807, 978, 1413 |
| BspCNI | CTCAG | 1 cut(s) | 916 |
| BspEI | TCCGGA | 1 cut(s) | 1193 |
| BspLI | GGNNCC | 2 cut(s) | 229, 658 |
| BspOI | GCTAGC | 1 cut(s) | 1020 |
| BspPI | GGATC | 3 cut(s) | 604, 1254, 1289 |
| BspT107I | GGYRCC | 1 cut(s) | 656 |
| BsrDI | GCAATG | 2 cut(s) | 1005, 1326 |
| BsrFI | RCCGGY | 1 cut(s) | 224 |
| BsrI | ACTGG | 5 cut(s) | 90, 96, 177, 630, 715 |
| BssAI | RCCGGY | 1 cut(s) | 224 |
| BssECI | CCNNGG | 2 cut(s) | 398, 664 |
| BssMI | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| BssSI | CACGAG | 1 cut(s) | 675 |
| BssT1I | CCWWGG | 2 cut(s) | 398, 664 |
| Bst2BI | CACGAG | 1 cut(s) | 675 |
| Bst2UI | CCWGG | 2 cut(s) | 498, 1074 |
| Bst4CI | ACNGT | 5 cut(s) | 364, 511, 1034, 1367, 1503 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1004 |
| BstC8I | GCNNGC | 8 cut(s) | 37, 73, 209, 465, 533, 909, 1018, 1431 |
| BstDEI | CTNAG | 2 cut(s) | 262, 903 |
| BstEII | GGTNACC | 1 cut(s) | 1453 |
| BstF5I | GGATG | 8 cut(s) | 273, 361, 397, 639, 660, 711, 852, 1192 |
| BstKTI | GATC | 8 cut(s) | 130, 283, 612, 872, 1249, 1297, 1311, 1482 |
| BstMAI | GTCTC | 3 cut(s) | 47, 63, 1017 |
| BstMBI | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| BstMCI | CGRYCG | 1 cut(s) | 172 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 77, 234, 908, 917, 1004, 1013, 1145, 1367, 1619 |
| BstNI | CCWGG | 2 cut(s) | 498, 1074 |
| BstNSI | RCATGY | 5 cut(s) | 467, 471, 483, 547, 1433 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1035 |
| BstPI | GGTNACC | 1 cut(s) | 1453 |
| BstSCI | CCNGG | 3 cut(s) | 496, 855, 1072 |
| BstSFI | CTRYAG | 1 cut(s) | 982 |
| BstSLI | GKGCMC | 1 cut(s) | 1501 |
| BstV1I | GCAGC | 3 cut(s) | 67, 92, 932 |
| BstX2I | RGATCY | 2 cut(s) | 869, 1294 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 392, 548, 703 |
| BstYI | RGATCY | 2 cut(s) | 869, 1294 |
| BstZI | CGGCCG | 1 cut(s) | 169 |
| BsuI | GTATCC | 3 cut(s) | 303, 575, 1385 |
| BsuRI | GGCC | 7 cut(s) | 171, 221, 228, 684, 807, 978, 1413 |
| BtgZI | GCGATG | 1 cut(s) | 1397 |
| BtrI | CACGTC | 1 cut(s) | 431 |
| BtsCI | GGATG | 8 cut(s) | 273, 361, 397, 639, 660, 711, 852, 1192 |
| BtsI | GCAGTG | 1 cut(s) | 885 |
| BtsIMutI | CAGTG | 5 cut(s) | 184, 623, 885, 1372, 1482 |
| Cac8I | GCNNGC | 8 cut(s) | 37, 73, 209, 465, 533, 909, 1018, 1431 |
| Cfr10I | RCCGGY | 1 cut(s) | 224 |
| Cfr13I | GGNCC | 6 cut(s) | 227, 395, 425, 976, 1070, 1412 |
| CseI | GACGC | 3 cut(s) | 415, 426, 1611 |
| CsiI | ACCWGGT | 1 cut(s) | 1072 |
| Csp6I | GTAC | 2 cut(s) | 718, 743 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 634, 669 |
| CviQI | GTAC | 2 cut(s) | 718, 743 |
| DdeI | CTNAG | 2 cut(s) | 262, 903 |
| DpnI | GATC | 8 cut(s) | 129, 282, 611, 871, 1248, 1296, 1310, 1481 |
| DpnII | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1621 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1621 |
| EaeI | YGGCCR | 3 cut(s) | 169, 219, 682 |
| EagI | CGGCCG | 1 cut(s) | 169 |
| EclXI | CGGCCG | 1 cut(s) | 169 |
| Eco130I | CCWWGG | 2 cut(s) | 398, 664 |
| Eco24I | GRGCYC | 1 cut(s) | 213 |
| Eco47I | GGWCC | 3 cut(s) | 395, 425, 1070 |
| Eco52I | CGGCCG | 1 cut(s) | 169 |
| Eco57I | CTGAAG | 2 cut(s) | 1127, 1602 |
| Eco88I | CYCGRG | 1 cut(s) | 60 |
| Eco91I | GGTNACC | 1 cut(s) | 1453 |
| EcoO65I | GGTNACC | 1 cut(s) | 1453 |
| EcoRI | GAATTC | 2 cut(s) | 561, 828 |
| EcoRII | CCWGG | 2 cut(s) | 496, 1072 |
| EcoT14I | CCWWGG | 2 cut(s) | 398, 664 |
| EcoT22I | ATGCAT | 1 cut(s) | 469 |
| EcoT38I | GRGCYC | 1 cut(s) | 213 |
| ErhI | CCWWGG | 2 cut(s) | 398, 664 |
| Esp3I | CGTCTC | 1 cut(s) | 1017 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1146, 1178, 1417, 1449 |
| FaqI | GGGAC | 3 cut(s) | 78, 418, 682 |
| FauI | CCCGC | 3 cut(s) | 153, 524, 1176 |
| FbaI | TGATCA | 1 cut(s) | 1479 |
| FblI | GTMKAC | 2 cut(s) | 152, 1029 |
| Fnu4HI | GCNGC | 6 cut(s) | 81, 106, 235, 685, 921, 979 |
| FokI | GGATG | 8 cut(s) | 260, 368, 404, 626, 647, 698, 839, 1199 |
| FriOI | GRGCYC | 1 cut(s) | 213 |
| Fsp4HI | GCNGC | 6 cut(s) | 81, 106, 235, 685, 921, 979 |
| FspBI | CTAG | 2 cut(s) | 1017, 1626 |
| GluI | GCNGC | 6 cut(s) | 81, 106, 235, 685, 921, 979 |
| HaeIII | GGCC | 7 cut(s) | 171, 221, 228, 684, 807, 978, 1413 |
| HapII | CCGG | 3 cut(s) | 225, 857, 1194 |
| HgaI | GACGC | 3 cut(s) | 415, 426, 1611 |
| HincII | GTYRAC | 1 cut(s) | 1420 |
| HindII | GTYRAC | 1 cut(s) | 1420 |
| HindIII | AAGCTT | 3 cut(s) | 940, 1202, 1528 |
| HinfI | GANTC | 7 cut(s) | 121, 449, 990, 1042, 1049, 1318, 1401 |
| HpaI | GTTAAC | 1 cut(s) | 1420 |
| HpaII | CCGG | 3 cut(s) | 225, 857, 1194 |
| HphI | GGTGA | 1 cut(s) | 1465 |
| Hpy166II | GTNNAC | 5 cut(s) | 153, 706, 1030, 1079, 1420 |
| Hpy188I | TCNGA | 5 cut(s) | 199, 435, 569, 1041, 1336 |
| Hpy8I | GTNNAC | 5 cut(s) | 153, 706, 1030, 1079, 1420 |
| Hpy99I | CGWCG | 1 cut(s) | 420 |
| HpyAV | CCTTC | 6 cut(s) | 430, 1061, 1102, 1136, 1419, 1627 |
| HpyCH4III | ACNGT | 5 cut(s) | 364, 511, 1034, 1367, 1503 |
| HpyCH4IV | ACGT | 4 cut(s) | 240, 290, 430, 1026 |
| HpyCH4V | TGCA | 7 cut(s) | 463, 467, 920, 1148, 1210, 1331, 1350 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 77, 234, 908, 917, 1004, 1013, 1145, 1367, 1619 |
| HpyF3I | CTNAG | 2 cut(s) | 262, 903 |
| HpySE526I | ACGT | 4 cut(s) | 240, 290, 430, 1026 |
| Kpn2I | TCCGGA | 1 cut(s) | 1193 |
| Ksp22I | TGATCA | 1 cut(s) | 1479 |
| KspAI | GTTAAC | 1 cut(s) | 1420 |
| Kzo9I | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| LmnI | GCTCC | 2 cut(s) | 80, 770 |
| Lsp1109I | GCAGC | 3 cut(s) | 67, 92, 932 |
| LweI | GCATC | 3 cut(s) | 720, 1177, 1340 |
| MabI | ACCWGGT | 1 cut(s) | 1072 |
| MaeI | CTAG | 2 cut(s) | 1017, 1626 |
| MaeII | ACGT | 4 cut(s) | 240, 290, 430, 1026 |
| MaeIII | GTNAC | 3 cut(s) | 475, 1118, 1453 |
| MalI | GATC | 8 cut(s) | 129, 282, 611, 871, 1248, 1296, 1310, 1481 |
| MboI | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| MboII | GAAGA | 3 cut(s) | 260, 1154, 1573 |
| MflI | RGATCY | 2 cut(s) | 869, 1294 |
| MhlI | GDGCHC | 2 cut(s) | 213, 1501 |
| MlsI | TGGCCA | 1 cut(s) | 221 |
| MluCI | AATT | 4 cut(s) | 192, 561, 726, 828 |
| MluNI | TGGCCA | 1 cut(s) | 221 |
| MlyI | GAGTC | 2 cut(s) | 115, 458 |
| MmeI | TCCRAC | 1 cut(s) | 222 |
| Mox20I | TGGCCA | 1 cut(s) | 221 |
| Mph1103I | ATGCAT | 1 cut(s) | 469 |
| MroI | TCCGGA | 1 cut(s) | 1193 |
| MroXI | GAANNNNTTC | 1 cut(s) | 252 |
| MscI | TGGCCA | 1 cut(s) | 221 |
| MseI | TTAA | 6 cut(s) | 333, 617, 729, 915, 971, 1419 |
| MslI | CAYNNNNRTG | 3 cut(s) | 546, 998, 1304 |
| Msp20I | TGGCCA | 1 cut(s) | 221 |
| MspA1I | CMGCKG | 3 cut(s) | 80, 105, 1183 |
| MspI | CCGG | 3 cut(s) | 225, 857, 1194 |
| MspR9I | CCNGG | 3 cut(s) | 498, 857, 1074 |
| MvaI | CCWGG | 2 cut(s) | 498, 1074 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 77, 234, 908, 917, 1004, 1013, 1145, 1367, 1619 |
| NciI | CCSGG | 1 cut(s) | 857 |
| NdeII | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| NheI | GCTAGC | 1 cut(s) | 1016 |
| NlaIV | GGNNCC | 2 cut(s) | 229, 658 |
| NmeAIII | GCCGAG | 1 cut(s) | 1046 |
| NmuCI | GTSAC | 3 cut(s) | 475, 1118, 1453 |
| NsiI | ATGCAT | 1 cut(s) | 469 |
| NspI | RCATGY | 5 cut(s) | 467, 471, 483, 547, 1433 |
| PaeI | GCATGC | 2 cut(s) | 467, 1433 |
| PaeR7I | CTCGAG | 1 cut(s) | 60 |
| PciI | ACATGT | 2 cut(s) | 479, 543 |
| PdmI | GAANNNNTTC | 1 cut(s) | 252 |
| PfeI | GAWTC | 5 cut(s) | 990, 1042, 1049, 1318, 1401 |
| PflFI | GACNNNGTC | 1 cut(s) | 429 |
| PfoI | TCCNGGA | 1 cut(s) | 496 |
| PkrI | GCNGC | 6 cut(s) | 82, 107, 236, 686, 922, 980 |
| PleI | GAGTC | 2 cut(s) | 115, 457 |
| PpsI | GAGTC | 2 cut(s) | 115, 457 |
| PscI | ACATGT | 2 cut(s) | 479, 543 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1035 |
| PshBI | ATTAAT | 2 cut(s) | 729, 915 |
| Psp6I | CCWGG | 2 cut(s) | 496, 1072 |
| PspEI | GGTNACC | 1 cut(s) | 1453 |
| PspGI | CCWGG | 2 cut(s) | 496, 1072 |
| PspN4I | GGNNCC | 2 cut(s) | 229, 658 |
| PspPI | GGNCC | 6 cut(s) | 227, 395, 425, 976, 1070, 1412 |
| PsuI | RGATCY | 2 cut(s) | 869, 1294 |
| PsyI | GACNNNGTC | 1 cut(s) | 429 |
| RsaI | GTAC | 2 cut(s) | 719, 744 |
| RsaNI | GTAC | 2 cut(s) | 718, 743 |
| RseI | CAYNNNNRTG | 3 cut(s) | 546, 998, 1304 |
| SaqAI | TTAA | 6 cut(s) | 333, 617, 729, 915, 971, 1419 |
| SatI | GCNGC | 6 cut(s) | 81, 106, 235, 685, 921, 979 |
| Sau3AI | GATC | 8 cut(s) | 127, 280, 609, 869, 1246, 1294, 1308, 1479 |
| Sau96I | GGNCC | 6 cut(s) | 227, 395, 425, 976, 1070, 1412 |
| SchI | GAGTC | 2 cut(s) | 115, 458 |
| ScrFI | CCNGG | 3 cut(s) | 498, 857, 1074 |
| SduI | GDGCHC | 2 cut(s) | 213, 1501 |
| SexAI | ACCWGGT | 1 cut(s) | 1072 |
| SfaNI | GCATC | 3 cut(s) | 720, 1177, 1340 |
| SfcI | CTRYAG | 1 cut(s) | 982 |
| Sfr274I | CTCGAG | 1 cut(s) | 60 |
| SinI | GGWCC | 3 cut(s) | 395, 425, 1070 |
| SlaI | CTCGAG | 1 cut(s) | 60 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 546, 998, 1304 |
| SmlI | CTYRAG | 1 cut(s) | 60 |
| SmoI | CTYRAG | 1 cut(s) | 60 |
| SphI | GCATGC | 2 cut(s) | 467, 1433 |
| Sse9I | AATT | 4 cut(s) | 192, 561, 726, 828 |
| SspI | AATATT | 1 cut(s) | 1240 |
| SspMI | CTAG | 2 cut(s) | 1017, 1626 |
| StyD4I | CCNGG | 3 cut(s) | 496, 855, 1072 |
| StyI | CCWWGG | 2 cut(s) | 398, 664 |
| TaaI | ACNGT | 5 cut(s) | 364, 511, 1034, 1367, 1503 |
| TaiI | ACGT | 4 cut(s) | 243, 293, 433, 1029 |
| TaqI | TCGA | 7 cut(s) | 61, 111, 246, 415, 882, 1256, 1551 |
| TaqII | GACCGA | 1 cut(s) | 1472 |
| TasI | AATT | 4 cut(s) | 192, 561, 726, 828 |
| TatI | WGTACW | 2 cut(s) | 717, 742 |
| TauI | GCSGC | 3 cut(s) | 237, 687, 981 |
| TfiI | GAWTC | 5 cut(s) | 990, 1042, 1049, 1318, 1401 |
| Tru1I | TTAA | 6 cut(s) | 333, 617, 729, 915, 971, 1419 |
| Tru9I | TTAA | 6 cut(s) | 333, 617, 729, 915, 971, 1419 |
| TscAI | CASTG | 5 cut(s) | 184, 630, 892, 1372, 1489 |
| TseFI | GTSAC | 3 cut(s) | 475, 1118, 1453 |
| TseI | GCWGC | 3 cut(s) | 80, 105, 920 |
| Tsp45I | GTSAC | 3 cut(s) | 475, 1118, 1453 |
| TspDTI | ATGAA | 5 cut(s) | 982, 1101, 1190, 1364, 1436 |
| TspGWI | ACGGA | 2 cut(s) | 1431, 1437 |
| TspRI | CASTG | 5 cut(s) | 184, 630, 892, 1372, 1489 |
| Tth111I | GACNNNGTC | 1 cut(s) | 429 |
| VpaK11BI | GGWCC | 3 cut(s) | 395, 425, 1070 |
| VspI | ATTAAT | 2 cut(s) | 729, 915 |
| XapI | RAATTY | 2 cut(s) | 561, 828 |
| XbaI | TCTAGA | 1 cut(s) | 1625 |
| XceI | RCATGY | 5 cut(s) | 467, 471, 483, 547, 1433 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 184, 650, 1589 |
| XhoI | CTCGAG | 1 cut(s) | 60 |
| XmiI | GTMKAC | 2 cut(s) | 152, 1029 |
| XmnI | GAANNNNTTC | 1 cut(s) | 252 |
| XspI | CTAG | 2 cut(s) | 1017, 1626 |
| Zsp2I | ATGCAT | 1 cut(s) | 469 |