pycom17g21600

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001282
Physical Location & Seq
Forward (+)
19978774 .. 19979788
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
ATGGAGAGCAAGATGGTGATTGTTTTCTGTGTAGTTGTGGCTGTGTTCCTGCAAAATGTTGCAGCACAGACGGGACATGTGGTGGGAGGAAGCATAGGTTGGACCATCCCTCAATCTGGTGCACAAGAGTATGTCACTTGGGCTGCAAGTAACAAGTTCGTTGTTAGTGATACTCTCACTTTCAACTTCGCCACCAACGCACACGATGTACAAGAAGTACCCAAAGCATCATTCGACTCATGCAGTTCGGAAAACCCAATTGGCACGGCTATCACAACTGGCCCAGCAAACATCACTCTCACCTCTTCAGGCGACCACTACTACATCTGCACTTATAGCCGTCATTGCCAGTCGGGCCAGAAACTAGCCATCACCGTCTCAGCTGCCTCCCCAGGTGCCGCACCTTCTGTACCACCACCACCCTCTACCACTAACACCCCTCCAACTACCCCTTCCCCTACATCAAATGACCCTGCCGCTTGCGCACCAGTCCCAGCTTCATCACCATCCATGAATGGGGGAGTACCCACCGTGAACAACACCCCGGGGTCGCCGCCTCCGCTGGGCTCTTCTTCACCTGCTGTTTTGGCTAGTTTTTCTTTGTCGGCTGCTGCTCTTATCGTGGGTTTGCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

213

Amino Acids

21.43

Weight (kDa)

5.32

Isoelectric Point (pI)

60.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 120 1.4e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 586
Acc16I TGCGCA 1 cut(s) 484
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 395
AciI CCGC 4 cut(s) 399, 477, 554, 560
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 4 cut(s) 210, 219, 411, 525
AfiI CCNNNNNNNGG 3 cut(s) 116, 516, 563
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 1 cut(s) 391
AjuI GAANNNNNNNTTGG 8 cut(s) 82, 114, 215, 243, 247, 275, 436, 468
AluBI AGCT 2 cut(s) 383, 497
AluI AGCT 2 cut(s) 383, 497
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 1 cut(s) 382
Alw44I GTGCAC 1 cut(s) 120
Ama87I CYCGRG 1 cut(s) 544
AoxI GGCC 2 cut(s) 280, 355
ApaLI GTGCAC 1 cut(s) 120
ApeKI GCWGC 5 cut(s) 62, 143, 383, 608, 611
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 102, 281, 355
AsuC2I CCSGG 2 cut(s) 545, 546
AsuHPI GGTGA 5 cut(s) 28, 292, 364, 495, 567
AvaI CYCGRG 1 cut(s) 544
AvaII GGWCC 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 5 cut(s) 74, 130, 370, 595, 598
BccI CCATC 4 cut(s) 7, 113, 377, 514
BceAI ACGGC 2 cut(s) 282, 324
BciT130I CCWGG 1 cut(s) 393
BcnI CCSGG 2 cut(s) 545, 546
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 2 cut(s) 365, 591
BfuAI ACCTGC 1 cut(s) 586
BglI GCCNNNNNGGC 1 cut(s) 354
BisI GCNGC 8 cut(s) 63, 144, 384, 399, 477, 554, 609, 612
BlsI GCNGC 8 cut(s) 64, 145, 385, 400, 478, 555, 610, 613
Bme1390I CCNGG 3 cut(s) 393, 545, 546
Bme18I GGWCC 1 cut(s) 102
BmeT110I CYCGRG 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 102, 281, 355
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 3 cut(s) 393, 545, 546
BmsI GCATC 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 545, 546
BsaJI CCNNGG 4 cut(s) 391, 543, 544, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 516, 563
Bse1I ACTGG 3 cut(s) 283, 349, 488
Bse3DI GCAATG 1 cut(s) 343
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 4 cut(s) 391, 543, 544, 545
BseGI GGATG 2 cut(s) 105, 506
BseLI CCNNNNNNNGG 3 cut(s) 116, 516, 563
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 3 cut(s) 283, 349, 488
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 5 cut(s) 74, 130, 370, 595, 598
BseYI CCCAGC 3 cut(s) 283, 493, 562
BsgI GTGCAG 1 cut(s) 313
BshFI GGCC 2 cut(s) 282, 357
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 2 cut(s) 87, 476
BslI CCNNNNNNNGG 3 cut(s) 116, 516, 563
BsmAI GTCTC 1 cut(s) 382
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 2 cut(s) 87, 476
BsnI GGCC 2 cut(s) 282, 357
BsoBI CYCGRG 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 124, 569
Bsp1407I TGTACA 1 cut(s) 208
BspACI CCGC 4 cut(s) 399, 477, 554, 560
BspANI GGCC 2 cut(s) 282, 357
BspCNI CTCAG 1 cut(s) 392
BspLI GGNNCC 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 586
BspQI GCTCTTC 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 343
BsrGI TGTACA 1 cut(s) 208
BsrI ACTGG 3 cut(s) 283, 349, 488
BssECI CCNNGG 4 cut(s) 391, 543, 544, 545
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 376, 532
Bst6I CTCTTC 2 cut(s) 310, 574
BstAUI TGTACA 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 481
BstDEI CTNAG 1 cut(s) 379
BstF5I GGATG 2 cut(s) 105, 506
BstHHI GCGC 1 cut(s) 485
BstMAI GTCTC 1 cut(s) 382
BstMWI GCNNNNNNNGC 7 cut(s) 197, 336, 345, 354, 482, 559, 587
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 3 cut(s) 391, 543, 544
BstSLI GKGCMC 1 cut(s) 124
BstV1I GCAGC 5 cut(s) 74, 130, 370, 595, 598
BsuRI GGCC 2 cut(s) 282, 357
BtsCI GGATG 2 cut(s) 105, 506
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 1 cut(s) 481
CfoI GCGC 1 cut(s) 485
Cfr13I GGNCC 3 cut(s) 102, 281, 355
Cfr9I CCCGGG 1 cut(s) 544
Csp6I GTAC 4 cut(s) 209, 218, 410, 524
CviAII CATG 3 cut(s) 77, 240, 511
CviQI GTAC 4 cut(s) 209, 218, 410, 524
DdeI CTNAG 1 cut(s) 379
Eam1104I CTCTTC 2 cut(s) 310, 574
EarI CTCTTC 2 cut(s) 310, 574
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 569
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 3 cut(s) 80, 243, 514
FaiI YATR 6 cut(s) 78, 95, 132, 241, 336, 512
FaqI GGGAC 2 cut(s) 87, 476
FatI CATG 3 cut(s) 76, 239, 510
Fnu4HI GCNGC 8 cut(s) 63, 144, 384, 399, 477, 554, 609, 612
FokI GGATG 2 cut(s) 92, 493
FriOI GRGCYC 1 cut(s) 569
Fsp4HI GCNGC 8 cut(s) 63, 144, 384, 399, 477, 554, 609, 612
FspBI CTAG 2 cut(s) 365, 591
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 1 cut(s) 484
GluI GCNGC 8 cut(s) 63, 144, 384, 399, 477, 554, 609, 612
GsaI CCCAGC 3 cut(s) 287, 497, 566
HaeIII GGCC 2 cut(s) 282, 357
HapII CCGG 1 cut(s) 545
HhaI GCGC 1 cut(s) 485
Hin1II CATG 3 cut(s) 80, 243, 514
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HinfI GANTC 1 cut(s) 236
HpaII CCGG 1 cut(s) 545
HphI GGTGA 5 cut(s) 28, 292, 364, 495, 567
Hpy166II GTNNAC 2 cut(s) 122, 535
Hpy188I TCNGA 1 cut(s) 250
Hpy8I GTNNAC 2 cut(s) 122, 535
HpyAV CCTTC 2 cut(s) 414, 462
HpyCH4III ACNGT 2 cut(s) 376, 532
HpyCH4V TGCA 6 cut(s) 52, 62, 122, 146, 243, 330
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 336, 345, 354, 482, 559, 587
HpyF3I CTNAG 1 cut(s) 379
Hsp92II CATG 3 cut(s) 80, 243, 514
HspAI GCGC 1 cut(s) 483
LguI GCTCTTC 1 cut(s) 574
Lsp1109I GCAGC 5 cut(s) 74, 130, 370, 595, 598
LweI GCATC 1 cut(s) 236
MaeI CTAG 2 cut(s) 365, 591
MaeIII GTNAC 2 cut(s) 133, 149
MboII GAAGA 3 cut(s) 297, 561, 564
MfeI CAATTG 1 cut(s) 258
MhlI GDGCHC 2 cut(s) 124, 569
MluCI AATT 1 cut(s) 258
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 80, 467
MnlI CCTC 7 cut(s) 80, 120, 313, 397, 433, 450, 567
MspA1I CMGCKG 2 cut(s) 383, 562
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 3 cut(s) 393, 545, 546
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 7 cut(s) 197, 336, 345, 354, 482, 559, 587
NciI CCSGG 2 cut(s) 545, 546
NlaIII CATG 3 cut(s) 80, 243, 514
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 1 cut(s) 133
NsbI TGCGCA 1 cut(s) 484
NspI RCATGY 1 cut(s) 80
PaqCI CACCTGC 1 cut(s) 586
PciI ACATGT 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 574
PkrI GCNGC 8 cut(s) 64, 145, 385, 400, 478, 555, 610, 613
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PscI ACATGT 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 391
PspFI CCCAGC 3 cut(s) 283, 493, 562
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 3 cut(s) 102, 281, 355
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 4 cut(s) 210, 219, 411, 525
RsaNI GTAC 4 cut(s) 209, 218, 410, 524
SapI GCTCTTC 1 cut(s) 574
SatI GCNGC 8 cut(s) 63, 144, 384, 399, 477, 554, 609, 612
Sau96I GGNCC 3 cut(s) 102, 281, 355
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 3 cut(s) 393, 545, 546
SduI GDGCHC 2 cut(s) 124, 569
SetI ASST 7 cut(s) 100, 305, 385, 397, 406, 499, 580
SfaNI GCATC 1 cut(s) 236
SinI GGWCC 1 cut(s) 102
SmaI CCCGGG 1 cut(s) 546
Sse9I AATT 1 cut(s) 258
SsiI CCGC 4 cut(s) 399, 477, 554, 560
SspMI CTAG 2 cut(s) 365, 591
StyD4I CCNGG 3 cut(s) 391, 543, 544
TaaI ACNGT 2 cut(s) 376, 532
TaqI TCGA 1 cut(s) 234
TasI AATT 1 cut(s) 258
TatI WGTACW 1 cut(s) 208
TauI GCSGC 3 cut(s) 401, 479, 556
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 5 cut(s) 62, 143, 383, 608, 611
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 489, 527
TspMI CCCGGG 1 cut(s) 544
VneI GTGCAC 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 102
XceI RCATGY 1 cut(s) 80
XmaI CCCGGG 1 cut(s) 544
XspI CTAG 2 cut(s) 365, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.