RchiOBHm_Chr2g0167821

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000757
Physical Location & Seq
Reverse (-)
82155885 .. 82156901
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGCAGAACCATCTGGTGCCCAATCCCCACCCTATTATTCTTCCAGTCCCTGGAATAATCAATCGGGTTTGGCACAAGAATTTGGAGTCTGAGTTTAAGAAAATCCGGAACTTACTCTTTTGGCCCGGTGAGCCCCATACGCATGACCGCTGCTTTCTTGCTTCTATAGACACCGAATTCCCCGGTCAACTTTCTGAATCCGACAAAGGCCGTCTCCGAGAGCCCCAGTACAATTATGAGATGATGAAGAACAATGTGAACGCCACCAACATAATCCAATTGGGTTTGACTCTCTCGGACAGATTCGGAAACTTCTACGTATGGGAGTTCAATTTCAGTGACTTTGATATCGAAAGTGATAATCAAAACAAGGTCTCAATTGAGTTGCTCAAGACTCATGGGACCGATTTCCAGAGAAACAAGAAGGATGGTATTCCTTCCTCTAAGTTCGCAGAGTTGTGTCGGGGCTCTGGCCTGGTAGGAAACAGTTCAGAGGTACATTTGACATGGGTGGGTTTCCAGAGTTCTTATGATTTTGGCTTCTTCACCAAGATTCTCAGTGGGCAAAAGTTGCCAGACAACATGCATGGATTCATGAGAAATGTGAAGAACTACTTTGGGCCTAGCGTGTTTGACATGAAACACATGATCACGAGGTTCTGTTCTGGAATTTATGGGGGTTTGGAGAAGGTGGCAGAGACGCTTGGGGTTGCTCGGGCAGCCGGGACGAGTCACATGGCTGGCTCTGACAGTCTTTTGACATTGCAAACCTTTCTCAAGCTTATAAATGTGTGTTTGAAGCCAGCAGATTCTAACAGCAGCAGCATTGAGACATTGCAGAAGTTTACAATGAATATGAATTTTGTGAATTCTTTTGTGAGCTCCCGCGCTCCTTGTGATACCGGTTTGGCAATGCAGGTCTTTAAGATGCTTATAAGACTCGAGGAAGAAAGAGAGAAACAGAACTACTTTAAATTGTATAGTTGTCAAGGGGCTTTGTATAGTTTAGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

338

Amino Acids

38.36

Weight (kDa)

7.66

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 51 - 146 1.7e-09 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 785, 935
Acc36I ACCTGC 1 cut(s) 907
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 50
AccII CGCG 1 cut(s) 888
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 2 cut(s) 148, 886
AcsI RAATTY 5 cut(s) 79, 176, 669, 859, 868
AfaI GTAC 2 cut(s) 230, 498
AfiI CCNNNNNNNGG 1 cut(s) 50
AgeI ACCGGT 1 cut(s) 902
AgsI TTSAA 2 cut(s) 331, 799
AjnI CCWGG 2 cut(s) 49, 474
AluBI AGCT 2 cut(s) 781, 882
AluI AGCT 2 cut(s) 781, 882
Alw21I GWGCWC 1 cut(s) 884
Alw26I GTCTC 4 cut(s) 218, 379, 692, 824
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 2 cut(s) 714, 941
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 4 cut(s) 122, 208, 472, 620
ApeKI GCWGC 4 cut(s) 150, 719, 819, 822
ApoI RAATTY 5 cut(s) 79, 176, 669, 859, 868
AsiGI ACCGGT 1 cut(s) 902
Asp700I GAANNNNTTC 1 cut(s) 487
AspLEI GCGC 1 cut(s) 890
AspS9I GGNCC 3 cut(s) 123, 402, 620
AsuC2I CCSGG 3 cut(s) 126, 183, 724
AsuHPI GGTGA 2 cut(s) 140, 538
AvaI CYCGRG 2 cut(s) 714, 941
AvaII GGWCC 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 4 cut(s) 135, 225, 470, 884
BauI CACGAG 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 884
BbvI GCAGC 4 cut(s) 137, 731, 831, 834
BccI CCATC 2 cut(s) 18, 422
BceAI ACGGC 1 cut(s) 195
BciT130I CCWGG 2 cut(s) 51, 476
BclI TGATCA 1 cut(s) 648
BcnI CCSGG 3 cut(s) 126, 183, 724
BcoDI GTCTC 4 cut(s) 218, 379, 692, 824
BfaI CTAG 1 cut(s) 624
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 907
BisI GCNGC 4 cut(s) 151, 720, 820, 823
BlsI GCNGC 4 cut(s) 152, 721, 821, 824
Bme1390I CCNGG 5 cut(s) 51, 126, 183, 476, 724
Bme18I GGWCC 1 cut(s) 402
BmeT110I CYCGRG 2 cut(s) 714, 941
BmgT120I GGNCC 3 cut(s) 123, 402, 620
BmiI GGNNCC 2 cut(s) 18, 403
BmrFI CCNGG 5 cut(s) 51, 126, 183, 476, 724
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 1 cut(s) 918
BmuI ACTGGG 1 cut(s) 220
BpuEI CTTGAG 2 cut(s) 374, 761
BpuMI CCSGG 3 cut(s) 126, 183, 724
BsaAI YACGTR 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 49, 181
BsaWI WCCGGW 2 cut(s) 105, 902
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 902
Bse1I ACTGG 2 cut(s) 44, 226
Bse3DI GCAATG 3 cut(s) 761, 833, 918
BseAI TCCGGA 1 cut(s) 105
BseBI CCWGG 2 cut(s) 51, 476
BseDI CCNNGG 2 cut(s) 49, 181
BseGI GGATG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 3 cut(s) 761, 833, 918
BseMII CTCAG 2 cut(s) 81, 571
BseNI ACTGG 2 cut(s) 44, 226
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 4 cut(s) 137, 731, 831, 834
Bsh1236I CGCG 1 cut(s) 888
BshFI GGCC 4 cut(s) 124, 210, 474, 622
BshNI GGYRCC 1 cut(s) 16
BshTI ACCGGT 1 cut(s) 902
BsiHKAI GWGCWC 1 cut(s) 884
BsiHKCI CYCGRG 2 cut(s) 714, 941
BsiSI CCGG 5 cut(s) 106, 126, 183, 723, 903
BslFI GGGAC 3 cut(s) 32, 415, 739
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 4 cut(s) 218, 379, 692, 824
BsmBI CGTCTC 2 cut(s) 218, 692
BsmFI GGGAC 3 cut(s) 32, 415, 739
BsnI GGCC 4 cut(s) 124, 210, 474, 622
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 2 cut(s) 714, 941
Bsp1286I GDGCHC 5 cut(s) 21, 135, 225, 470, 884
Bsp13I TCCGGA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 648
BspACI CCGC 2 cut(s) 148, 886
BspANI GGCC 4 cut(s) 124, 210, 474, 622
BspCNI CTCAG 2 cut(s) 82, 570
BspEI TCCGGA 1 cut(s) 105
BspFNI CGCG 1 cut(s) 888
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 18, 403
BspMI ACCTGC 1 cut(s) 907
BspT107I GGYRCC 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 379
BsrDI GCAATG 3 cut(s) 761, 833, 918
BsrFI RCCGGY 1 cut(s) 902
BsrI ACTGG 2 cut(s) 44, 226
BssAI RCCGGY 1 cut(s) 902
BssECI CCNNGG 2 cut(s) 49, 181
BssMI GATC 1 cut(s) 648
BssSI CACGAG 1 cut(s) 652
Bst2BI CACGAG 1 cut(s) 652
Bst2UI CCWGG 2 cut(s) 51, 476
Bst4CI ACNGT 2 cut(s) 488, 752
BstAPI GCANNNNNTGC 1 cut(s) 571
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 742, 804
BstDEI CTNAG 3 cut(s) 90, 444, 557
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 1 cut(s) 888
BstHHI GCGC 1 cut(s) 890
BstKTI GATC 1 cut(s) 651
BstMAI GTCTC 4 cut(s) 218, 379, 692, 824
BstMBI GATC 1 cut(s) 648
BstMWI GCNNNNNNNGC 4 cut(s) 130, 139, 571, 719
BstNI CCWGG 2 cut(s) 51, 476
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 5 cut(s) 49, 124, 181, 474, 722
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 21
BstSNI TACGTA 1 cut(s) 319
BstUI CGCG 1 cut(s) 888
BstV1I GCAGC 4 cut(s) 137, 731, 831, 834
BsuRI GGCC 4 cut(s) 124, 210, 474, 622
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 343, 565
BveI ACCTGC 1 cut(s) 907
Cac8I GCNNGC 2 cut(s) 742, 804
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 594
CfoI GCGC 1 cut(s) 890
Cfr10I RCCGGY 1 cut(s) 902
Cfr13I GGNCC 3 cut(s) 123, 402, 620
CseI GACGC 1 cut(s) 709
Csp6I GTAC 2 cut(s) 229, 497
CspAI ACCGGT 1 cut(s) 902
CviAII CATG 9 cut(s) 143, 398, 507, 583, 587, 595, 637, 646, 736
CviQI GTAC 2 cut(s) 229, 497
DdeI CTNAG 3 cut(s) 90, 444, 557
DpnI GATC 1 cut(s) 650
DpnII GATC 1 cut(s) 648
DraI TTTAAA 1 cut(s) 973
Ecl136II GAGCTC 1 cut(s) 882
Eco105I TACGTA 1 cut(s) 319
Eco24I GRGCYC 4 cut(s) 135, 225, 470, 884
Eco31I GGTCTC 1 cut(s) 379
Eco32I GATATC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 882
Eco88I CYCGRG 2 cut(s) 714, 941
EcoICRI GAGCTC 1 cut(s) 882
EcoRI GAATTC 2 cut(s) 176, 868
EcoRII CCWGG 2 cut(s) 49, 474
EcoRV GATATC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 588
EcoT38I GRGCYC 4 cut(s) 135, 225, 470, 884
Esp3I CGTCTC 2 cut(s) 218, 692
FaeI CATG 9 cut(s) 146, 401, 510, 586, 590, 598, 640, 649, 739
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FaqI GGGAC 3 cut(s) 32, 415, 739
FatI CATG 9 cut(s) 142, 397, 506, 582, 586, 594, 636, 645, 735
FauI CCCGC 1 cut(s) 893
FbaI TGATCA 1 cut(s) 648
Fnu4HI GCNGC 4 cut(s) 151, 720, 820, 823
FokI GGATG 1 cut(s) 440
FriOI GRGCYC 4 cut(s) 135, 225, 470, 884
Fsp4HI GCNGC 4 cut(s) 151, 720, 820, 823
FspBI CTAG 1 cut(s) 624
GlaI GCGC 1 cut(s) 889
GluI GCNGC 4 cut(s) 151, 720, 820, 823
HaeIII GGCC 4 cut(s) 124, 210, 474, 622
HapII CCGG 5 cut(s) 106, 126, 183, 723, 903
HgaI GACGC 1 cut(s) 709
HhaI GCGC 1 cut(s) 890
Hin1II CATG 9 cut(s) 146, 401, 510, 586, 590, 598, 640, 649, 739
Hin6I GCGC 1 cut(s) 888
HinP1I GCGC 1 cut(s) 888
HincII GTYRAC 1 cut(s) 188
HindII GTYRAC 1 cut(s) 188
HindIII AAGCTT 1 cut(s) 779
HpaII CCGG 5 cut(s) 106, 126, 183, 723, 903
HphI GGTGA 2 cut(s) 140, 538
Hpy166II GTNNAC 3 cut(s) 188, 259, 846
Hpy188I TCNGA 8 cut(s) 91, 196, 202, 218, 298, 308, 493, 748
Hpy188III TCNNGA 7 cut(s) 106, 391, 412, 520, 595, 652, 666
Hpy8I GTNNAC 3 cut(s) 188, 259, 846
HpyAV CCTTC 3 cut(s) 418, 447, 682
HpyCH4III ACNGT 2 cut(s) 488, 752
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 5 cut(s) 4, 586, 766, 838, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 139, 571, 719
HpyF3I CTNAG 3 cut(s) 90, 444, 557
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 9 cut(s) 146, 401, 510, 586, 590, 598, 640, 649, 739
HspAI GCGC 1 cut(s) 888
Kpn2I TCCGGA 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 1 cut(s) 648
LmnI GCTCC 2 cut(s) 887, 895
Lsp1109I GCAGC 4 cut(s) 137, 731, 831, 834
LweI GCATC 1 cut(s) 918
MaeI CTAG 1 cut(s) 624
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 338, 731
MalI GATC 1 cut(s) 650
MboI GATC 1 cut(s) 648
MboII GAAGA 5 cut(s) 32, 259, 535, 619, 959
MfeI CAATTG 2 cut(s) 278, 378
MhlI GDGCHC 5 cut(s) 21, 135, 225, 470, 884
MlyI GAGTC 5 cut(s) 95, 283, 388, 739, 933
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 4 cut(s) 451, 487, 648, 937
Mph1103I ATGCAT 1 cut(s) 588
MroI TCCGGA 1 cut(s) 105
MroXI GAANNNNTTC 1 cut(s) 487
MseI TTAA 4 cut(s) 96, 924, 972, 1015
MslI CAYNNNNRTG 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 5 cut(s) 106, 126, 183, 723, 903
MspR9I CCNGG 5 cut(s) 51, 126, 183, 476, 724
MunI CAATTG 2 cut(s) 278, 378
MvaI CCWGG 2 cut(s) 51, 476
MvnI CGCG 1 cut(s) 888
MwoI GCNNNNNNNGC 4 cut(s) 130, 139, 571, 719
NciI CCSGG 3 cut(s) 126, 183, 724
NdeII GATC 1 cut(s) 648
NlaIII CATG 9 cut(s) 146, 401, 510, 586, 590, 598, 640, 649, 739
NlaIV GGNNCC 2 cut(s) 18, 403
NmuCI GTSAC 2 cut(s) 338, 731
NsiI ATGCAT 1 cut(s) 588
NspI RCATGY 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 941
PagI TCATGA 1 cut(s) 594
PdmI GAANNNNTTC 1 cut(s) 487
PfeI GAWTC 5 cut(s) 197, 303, 553, 591, 809
PflMI CCANNNNNTGG 1 cut(s) 50
PinAI ACCGGT 1 cut(s) 902
PkrI GCNGC 4 cut(s) 152, 721, 821, 824
PleI GAGTC 5 cut(s) 94, 283, 388, 738, 933
PpsI GAGTC 5 cut(s) 94, 283, 388, 738, 933
Ppu21I YACGTR 1 cut(s) 319
PsiI TTATAA 2 cut(s) 785, 935
Psp124BI GAGCTC 1 cut(s) 884
Psp6I CCWGG 2 cut(s) 49, 474
PspGI CCWGG 2 cut(s) 49, 474
PspN4I GGNNCC 2 cut(s) 18, 403
PspPI GGNCC 3 cut(s) 123, 402, 620
PspXI VCTCGAGB 1 cut(s) 941
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 2 cut(s) 230, 498
RsaNI GTAC 2 cut(s) 229, 497
RseI CAYNNNNRTG 1 cut(s) 141
SacI GAGCTC 1 cut(s) 884
SaqAI TTAA 4 cut(s) 96, 924, 972, 1015
SatI GCNGC 4 cut(s) 151, 720, 820, 823
Sau3AI GATC 1 cut(s) 648
Sau96I GGNCC 3 cut(s) 123, 402, 620
SchI GAGTC 5 cut(s) 95, 283, 388, 739, 933
ScrFI CCNGG 5 cut(s) 51, 126, 183, 476, 724
SduI GDGCHC 5 cut(s) 21, 135, 225, 470, 884
SetI ASST 9 cut(s) 321, 375, 498, 659, 693, 773, 783, 884, 921
SfaNI GCATC 1 cut(s) 918
SfcI CTRYAG 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 941
SinI GGWCC 1 cut(s) 402
SlaI CTCGAG 1 cut(s) 941
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 3 cut(s) 389, 776, 941
SmoI CTYRAG 3 cut(s) 389, 776, 941
SnaBI TACGTA 1 cut(s) 319
SsiI CCGC 2 cut(s) 148, 886
SspMI CTAG 1 cut(s) 624
SstI GAGCTC 1 cut(s) 884
StyD4I CCNGG 5 cut(s) 49, 124, 181, 474, 722
TaaI ACNGT 2 cut(s) 488, 752
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 351, 942
TaqII GACCGA 1 cut(s) 419
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 5 cut(s) 197, 303, 553, 591, 809
Tru1I TTAA 4 cut(s) 96, 924, 972, 1015
Tru9I TTAA 4 cut(s) 96, 924, 972, 1015
TscAI CASTG 2 cut(s) 343, 565
TseFI GTSAC 2 cut(s) 338, 731
TseI GCWGC 4 cut(s) 150, 719, 819, 822
Tsp45I GTSAC 2 cut(s) 338, 731
TspDTI ATGAA 5 cut(s) 260, 583, 653, 866, 872
TspRI CASTG 2 cut(s) 343, 565
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 402
XapI RAATTY 5 cut(s) 79, 176, 669, 859, 868
XceI RCATGY 1 cut(s) 586
XhoI CTCGAG 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 487
XspI CTAG 1 cut(s) 624
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.