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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 104, 189 |
| AgsI | TTSAA | 1 cut(s) | 44 |
| AoxI | GGCC | 1 cut(s) | 177 |
| ApoI | RAATTY | 2 cut(s) | 104, 189 |
| BseGI | GGATG | 1 cut(s) | 85 |
| BshFI | GGCC | 1 cut(s) | 179 |
| BsnI | GGCC | 1 cut(s) | 179 |
| Bsp143I | GATC | 1 cut(s) | 7 |
| BspANI | GGCC | 1 cut(s) | 179 |
| BssMI | GATC | 1 cut(s) | 7 |
| BstF5I | GGATG | 1 cut(s) | 85 |
| BstKTI | GATC | 1 cut(s) | 10 |
| BstMBI | GATC | 1 cut(s) | 7 |
| BsuRI | GGCC | 1 cut(s) | 179 |
| BtsCI | GGATG | 1 cut(s) | 85 |
| CviAII | CATG | 2 cut(s) | 120, 185 |
| CviJI | RGCY | 1 cut(s) | 179 |
| CviKI_1 | RGCY | 1 cut(s) | 179 |
| DpnI | GATC | 1 cut(s) | 9 |
| DpnII | GATC | 1 cut(s) | 7 |
| FaeI | CATG | 2 cut(s) | 123, 188 |
| FaiI | YATR | 3 cut(s) | 121, 140, 186 |
| FatI | CATG | 2 cut(s) | 119, 184 |
| FokI | GGATG | 1 cut(s) | 92 |
| HaeIII | GGCC | 1 cut(s) | 179 |
| Hin1II | CATG | 2 cut(s) | 123, 188 |
| Hpy166II | GTNNAC | 1 cut(s) | 94 |
| Hpy8I | GTNNAC | 1 cut(s) | 94 |
| HpyAV | CCTTC | 1 cut(s) | 38 |
| HpyCH4V | TGCA | 1 cut(s) | 147 |
| Hsp92II | CATG | 2 cut(s) | 123, 188 |
| Kzo9I | GATC | 1 cut(s) | 7 |
| LpnPI | CCDG | 2 cut(s) | 77, 95 |
| MaeIII | GTNAC | 1 cut(s) | 155 |
| MalI | GATC | 1 cut(s) | 9 |
| MboI | GATC | 1 cut(s) | 7 |
| MluCI | AATT | 2 cut(s) | 104, 189 |
| MnlI | CCTC | 1 cut(s) | 190 |
| NdeII | GATC | 1 cut(s) | 7 |
| NlaIII | CATG | 2 cut(s) | 123, 188 |
| Sau3AI | GATC | 1 cut(s) | 7 |
| SetI | ASST | 2 cut(s) | 49, 114 |
| SgeI | CNNG | 7 cut(s) | 29, 46, 76, 122, 132, 176, 187 |
| Sse9I | AATT | 2 cut(s) | 104, 189 |
| SspI | AATATT | 1 cut(s) | 53 |
| TaqI | TCGA | 1 cut(s) | 16 |
| TasI | AATT | 2 cut(s) | 104, 189 |
| XapI | RAATTY | 2 cut(s) | 104, 189 |