RchiOBHm_Chr5g0041221

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000569
Physical Location & Seq
Forward (+)
35934450 .. 35942835
8386 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31966

Sequence Viewer

Length: 1590 bp
ATGTCTCTATCATCCATCCATTCATCCATGTTGAGAATGGTTCTACAACTGCAATTGCTCCTCTTTCTTTGCTTCATAATTCTTCTCGGTATCCCTCTAAATCTTTCTTTAGCTTTTGATTTAGAACTAGGCAATGATGAGTTTAAAAGAGATGACTTCCCTCCCCCACCGGACTTTGTTTTTGGTGCTTCCTCCTCTGCTTACCAGGTGGAAGGTGCTGCGGACCAAGACGGGAGAACCCCAAGCATCTTGGACACCTATACTCATGCTGGAAAAATGCATGGAGCCACTGGAGATATAGCGTGTGATGAGTATCATAAATACAAGGAAGATGTGCAACTGATGGTGGATACTGGTTTAGAAGCGTATAGATTTTCCATCTCATGGTCAAGACTTATACCCAATGGAAGAGGACCCGTGAATCCAAAGGGTGTACAATATTACAACAATCTCATTGACCAACTAATCAGCAAAGGAATCCAGCCTCATGTTACTTTACACCACAGTGATCTCCCACAGGCACTCGACGATGAGTATGGAGGATGGGTTAGCCCAAAGATTGTAAAAGACTTCACTGCATATGCAAATGTTTGCTTCGAACAGTTTGGCGATCGAGTTGGGCATTGGACTACTATGAATGAGCCAAATGTATTTGTACTCGGGGGTTATGATATTGGATTCCTTCCACCACAACGATGTTCAGCTCCATTTGGTCTTAATTGCTCGAGGGGTAACTCCTCAACTGAGCCATACATGGCAATTCATTATTTCTTGTTATCTCATGCATCAGCAGCAGCACTCTACAAGCATACCTACCAGCACAAGCAGCGTGGGTTTATAGGCATCAATGTATTTGCCTATTGGTTTGTTCCTCAAACCGAAACCCATCAAGACCAAGTTGCTACTCAAAGAGCCCTCGACTTCTATTTTGGTTGGGTTATGCATCCTTTGGTGTATGGAGAGTACCCTCAAGTAATGAAAAAGAATGCAGGCTCTAGAATTCCAACGTTCACCAGTGCTGAGTCTTCAAAGGTCAAGGGTTCCTTCGACTTCATTGGACTTAATTACTATAACACATTGTATGCCAGTGACAACTCTGCCGCATTAAACATTCAAAACAGAGACTATGTAGCGGATTCGGCAACCCAGATTTCTCCTTTGGATTACAATACATCAACGTTGGAGTTTCCGATAACACCTTGGGGTATGGAAGGACTGTTGGAATATATCAAACAACGCTACGGCAATCCTCCCTTATATATCCATGAAAATGGCCAACAAACTCGACGTAATTCTTCGTTGGAAGATTGGTCCAGAATCGAATATTTGCATGGACACGTTCACAGTTTGCTTAATGTTATAAGGAATGGATCAAATGCTAGAGGCTATTTTACCTGGTCTCTCTTGGATTCAATTGAGCTTTTGGGTGGCTATGAAATAAGCTACGGCCTTTATTACATCGATTTGGACGATCCGGATTTAAAAAGACAGCCTAAACTCTCTGCACATTGGTACTCAAAGTTTCTAAAGAACAAAAACATCACTCCACGTTCAGATTCAGATGCTGATCAGCATTACTTCTTTCAGTAG

Protein Analysis

529

Amino Acids

60.09

Weight (kDa)

5.7

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 56 - 513 1.2e-138 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1361
AccB7I CCANNNNNTGG 1 cut(s) 384
AccIII TCCGGA 1 cut(s) 1474
AciI CCGC 3 cut(s) 221, 1101, 1133
AclI AACGTT 2 cut(s) 1007, 1178
AclWI GGATC 2 cut(s) 1378, 1466
AcoI YGGCCR 1 cut(s) 1273
AcsI RAATTY 1 cut(s) 999
AfaI GTAC 4 cut(s) 435, 657, 965, 1514
AfiI CCNNNNNNNGG 1 cut(s) 384
AflIII ACRYGT 1 cut(s) 1336
AgsI TTSAA 3 cut(s) 1029, 1115, 1413
AjnI CCWGG 2 cut(s) 204, 1394
AleI CACNNNNGTG 1 cut(s) 504
AluBI AGCT 4 cut(s) 113, 704, 1420, 1443
AluI AGCT 4 cut(s) 113, 704, 1420, 1443
Alw26I GTCTC 3 cut(s) 9, 1116, 1404
AlwI GGATC 2 cut(s) 1378, 1466
AlwNI CAGNNNCTG 1 cut(s) 1565
Ama87I CYCGRG 2 cut(s) 659, 724
Aor13HI TCCGGA 1 cut(s) 1474
AoxI GGCC 2 cut(s) 1273, 1447
ApeKI GCWGC 4 cut(s) 218, 791, 794, 826
ApoI RAATTY 1 cut(s) 999
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 911, 943
AspS9I GGNCC 3 cut(s) 223, 413, 1311
AsuHPI GGTGA 1 cut(s) 1003
AsuII TTCGAA 1 cut(s) 597
AvaI CYCGRG 2 cut(s) 659, 724
AvaII GGWCC 3 cut(s) 223, 413, 1311
BalI TGGCCA 1 cut(s) 1275
BanII GRGCYC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 1017
BbvI GCAGC 4 cut(s) 205, 803, 806, 838
BccI CCATC 5 cut(s) 23, 337, 386, 537, 894
BceAI ACGGC 2 cut(s) 1258, 1462
BciT130I CCWGG 2 cut(s) 206, 1396
BciVI GTATCC 2 cut(s) 101, 343
BclI TGATCA 1 cut(s) 1567
BcoDI GTCTC 3 cut(s) 9, 1116, 1404
BfaI CTAG 3 cut(s) 128, 996, 1380
BfuI GTATCC 2 cut(s) 101, 343
BisI GCNGC 5 cut(s) 219, 792, 795, 827, 1101
BlsI GCNGC 5 cut(s) 220, 793, 796, 828, 1102
Bme1390I CCNGG 2 cut(s) 206, 1396
Bme18I GGWCC 3 cut(s) 223, 413, 1311
BmeT110I CYCGRG 2 cut(s) 659, 724
BmgT120I GGNCC 3 cut(s) 223, 413, 1311
BmiI GGNNCC 3 cut(s) 286, 415, 1042
BmrFI CCNGG 2 cut(s) 206, 1396
BmsI GCATC 5 cut(s) 255, 794, 852, 952, 1552
BpiI GAAGAC 1 cut(s) 1017
BpmI CTGGAG 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 954
Bsa29I ATCGAT 1 cut(s) 1461
BsaBI GATNNNNATC 2 cut(s) 312, 1566
BsaI GGTCTC 1 cut(s) 1404
BsaJI CCNNGG 1 cut(s) 1199
BsaWI WCCGGW 2 cut(s) 169, 1474
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 4 cut(s) 295, 358, 1014, 1086
Bse3DI GCAATG 1 cut(s) 139
Bse8I GATNNNNATC 2 cut(s) 312, 1566
BseAI TCCGGA 1 cut(s) 1474
BseBI CCWGG 2 cut(s) 206, 1396
BseCI ATCGAT 1 cut(s) 1461
BseDI CCNNGG 1 cut(s) 1199
BseGI GGATG 5 cut(s) 11, 15, 23, 548, 943
BseJI GATNNNNATC 2 cut(s) 312, 1566
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 735, 1011
BseNI ACTGG 4 cut(s) 295, 358, 1014, 1086
BseRI GAGGAG 3 cut(s) 50, 184, 727
BseXI GCAGC 4 cut(s) 205, 803, 806, 838
BsgI GTGCAG 1 cut(s) 1488
Bsh1285I CGRYCG 1 cut(s) 613
BshFI GGCC 2 cut(s) 1275, 1449
BshVI ATCGAT 1 cut(s) 1461
BsiEI CGRYCG 1 cut(s) 613
BsiHKCI CYCGRG 2 cut(s) 659, 724
BsiSI CCGG 2 cut(s) 170, 1475
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 3 cut(s) 9, 1116, 1404
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 2 cut(s) 1275, 1449
Bso31I GGTCTC 1 cut(s) 1404
BsoBI CYCGRG 2 cut(s) 659, 724
Bsp119I TTCGAA 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 916
Bsp13I TCCGGA 1 cut(s) 1474
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 5 cut(s) 508, 610, 1370, 1471, 1567
BspACI CCGC 3 cut(s) 221, 1101, 1133
BspANI GGCC 2 cut(s) 1275, 1449
BspCNI CTCAG 2 cut(s) 736, 1012
BspDI ATCGAT 1 cut(s) 1461
BspEI TCCGGA 1 cut(s) 1474
BspLI GGNNCC 3 cut(s) 286, 415, 1042
BspPI GGATC 2 cut(s) 1378, 1466
BspT104I TTCGAA 1 cut(s) 597
BspTNI GGTCTC 1 cut(s) 1404
BsrDI GCAATG 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 4 cut(s) 295, 358, 1014, 1086
BssECI CCNNGG 1 cut(s) 1199
BssMI GATC 5 cut(s) 508, 610, 1370, 1471, 1567
BssT1I CCWWGG 1 cut(s) 1199
Bst2UI CCWGG 2 cut(s) 206, 1396
Bst4CI ACNGT 4 cut(s) 506, 603, 1218, 1346
Bst6I CTCTTC 1 cut(s) 403
BstAUI TGTACA 1 cut(s) 433
BstBI TTCGAA 1 cut(s) 597
BstC8I GCNNGC 1 cut(s) 991
BstDEI CTNAG 2 cut(s) 744, 1020
BstF5I GGATG 5 cut(s) 11, 15, 23, 548, 943
BstKTI GATC 5 cut(s) 511, 613, 1373, 1474, 1570
BstMAI GTCTC 3 cut(s) 9, 1116, 1404
BstMBI GATC 5 cut(s) 508, 610, 1370, 1471, 1567
BstMCI CGRYCG 1 cut(s) 613
BstMWI GCNNNNNNNGC 3 cut(s) 791, 826, 1139
BstNI CCWGG 2 cut(s) 206, 1396
BstSCI CCNGG 2 cut(s) 204, 1394
BstV1I GCAGC 4 cut(s) 205, 803, 806, 838
BstV2I GAAGAC 1 cut(s) 1017
BstXI CCANNNNNNTGG 1 cut(s) 1271
Bsu15I ATCGAT 1 cut(s) 1461
BsuI GTATCC 2 cut(s) 101, 343
BsuRI GGCC 2 cut(s) 1275, 1449
BsuTUI ATCGAT 1 cut(s) 1461
BtsCI GGATG 5 cut(s) 11, 15, 23, 548, 943
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 5 cut(s) 288, 511, 573, 1021, 1093
Cac8I GCNNGC 1 cut(s) 991
CaiI CAGNNNCTG 1 cut(s) 1565
Cfr13I GGNCC 3 cut(s) 223, 413, 1311
ClaI ATCGAT 1 cut(s) 1461
CsiI ACCWGGT 2 cut(s) 204, 1394
Csp6I GTAC 4 cut(s) 434, 656, 964, 1513
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviAII CATG 9 cut(s) 28, 266, 281, 384, 488, 754, 782, 1265, 1331
CviQI GTAC 4 cut(s) 434, 656, 964, 1513
DdeI CTNAG 2 cut(s) 744, 1020
DpnI GATC 5 cut(s) 510, 612, 1372, 1473, 1569
DpnII GATC 5 cut(s) 508, 610, 1370, 1471, 1567
DraI TTTAAA 2 cut(s) 145, 1482
EaeI YGGCCR 1 cut(s) 1273
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 1199
Eco24I GRGCYC 1 cut(s) 916
Eco31I GGTCTC 1 cut(s) 1404
Eco47I GGWCC 3 cut(s) 223, 413, 1311
Eco88I CYCGRG 2 cut(s) 659, 724
EcoO109I RGGNCCY 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 204, 1394
EcoT14I CCWWGG 1 cut(s) 1199
EcoT22I ATGCAT 3 cut(s) 282, 787, 945
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 1 cut(s) 1199
FaeI CATG 9 cut(s) 31, 269, 284, 387, 491, 757, 785, 1268, 1334
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FatI CATG 9 cut(s) 27, 265, 280, 383, 487, 753, 781, 1264, 1330
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 1 cut(s) 1567
Fnu4HI GCNGC 5 cut(s) 219, 792, 795, 827, 1101
FokI GGATG 4 cut(s) 2, 10, 555, 930
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 5 cut(s) 219, 792, 795, 827, 1101
FspBI CTAG 3 cut(s) 128, 996, 1380
GluI GCNGC 5 cut(s) 219, 792, 795, 827, 1101
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 2 cut(s) 1275, 1449
HapII CCGG 2 cut(s) 170, 1475
Hin1II CATG 9 cut(s) 31, 269, 284, 387, 491, 757, 785, 1268, 1334
HinfI GANTC 8 cut(s) 421, 477, 678, 1022, 1136, 1317, 1409, 1556
HpaII CCGG 2 cut(s) 170, 1475
HphI GGTGA 1 cut(s) 1003
Hpy166II GTNNAC 3 cut(s) 434, 1011, 1342
Hpy188I TCNGA 3 cut(s) 1191, 1555, 1561
Hpy188III TCNNGA 5 cut(s) 390, 890, 996, 1314, 1475
Hpy8I GTNNAC 3 cut(s) 434, 1011, 1342
Hpy99I CGWCG 2 cut(s) 530, 1290
HpyAV CCTTC 4 cut(s) 206, 692, 1054, 1205
HpyCH4III ACNGT 4 cut(s) 506, 603, 1218, 1346
HpyCH4IV ACGT 5 cut(s) 1007, 1178, 1288, 1338, 1549
HpyF10VI GCNNNNNNNGC 3 cut(s) 791, 826, 1139
HpyF3I CTNAG 2 cut(s) 744, 1020
HpySE526I ACGT 5 cut(s) 1007, 1178, 1288, 1338, 1549
Hsp92II CATG 9 cut(s) 31, 269, 284, 387, 491, 757, 785, 1268, 1334
Kpn2I TCCGGA 1 cut(s) 1474
Ksp22I TGATCA 1 cut(s) 1567
Kzo9I GATC 5 cut(s) 508, 610, 1370, 1471, 1567
LmnI GCTCC 3 cut(s) 63, 284, 709
Lsp1109I GCAGC 4 cut(s) 205, 803, 806, 838
LweI GCATC 5 cut(s) 255, 794, 852, 952, 1552
MabI ACCWGGT 2 cut(s) 204, 1394
MaeI CTAG 3 cut(s) 128, 996, 1380
MaeII ACGT 5 cut(s) 1007, 1178, 1288, 1338, 1549
MaeIII GTNAC 3 cut(s) 490, 731, 1088
MalI GATC 5 cut(s) 510, 612, 1372, 1473, 1569
MboI GATC 5 cut(s) 508, 610, 1370, 1471, 1567
MboII GAAGA 6 cut(s) 74, 341, 420, 1017, 1287, 1316
MfeI CAATTG 2 cut(s) 53, 1413
MhlI GDGCHC 1 cut(s) 916
MlsI TGGCCA 1 cut(s) 1275
MluCI AATT 8 cut(s) 53, 78, 718, 759, 999, 1063, 1291, 1413
MluNI TGGCCA 1 cut(s) 1275
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 4 cut(s) 1028, 1161, 1200, 1281
Mox20I TGGCCA 1 cut(s) 1275
Mph1103I ATGCAT 3 cut(s) 282, 787, 945
MroI TCCGGA 1 cut(s) 1474
MscI TGGCCA 1 cut(s) 1275
MseI TTAA 6 cut(s) 144, 717, 1062, 1106, 1353, 1481
MslI CAYNNNNRTG 3 cut(s) 504, 694, 1269
Msp20I TGGCCA 1 cut(s) 1275
MspI CCGG 2 cut(s) 170, 1475
MspR9I CCNGG 2 cut(s) 206, 1396
MunI CAATTG 2 cut(s) 53, 1413
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 2 cut(s) 206, 1396
MwoI GCNNNNNNNGC 3 cut(s) 791, 826, 1139
NdeI CATATG 1 cut(s) 580
NdeII GATC 5 cut(s) 508, 610, 1370, 1471, 1567
NlaIII CATG 9 cut(s) 31, 269, 284, 387, 491, 757, 785, 1268, 1334
NlaIV GGNNCC 3 cut(s) 286, 415, 1042
NmuCI GTSAC 1 cut(s) 1088
NsiI ATGCAT 3 cut(s) 282, 787, 945
NspV TTCGAA 1 cut(s) 597
OliI CACNNNNGTG 1 cut(s) 504
PaeR7I CTCGAG 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 1467
PctI GAATGC 1 cut(s) 991
PfeI GAWTC 7 cut(s) 421, 477, 678, 1136, 1317, 1409, 1556
PflMI CCANNNNNTGG 1 cut(s) 384
PkrI GCNGC 5 cut(s) 220, 793, 796, 828, 1102
Ple19I CGATCG 1 cut(s) 613
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
PpuMI RGGWCCY 1 cut(s) 413
PsiI TTATAA 1 cut(s) 1361
Psp1406I AACGTT 2 cut(s) 1007, 1178
Psp5II RGGWCCY 1 cut(s) 413
Psp6I CCWGG 2 cut(s) 204, 1394
PspGI CCWGG 2 cut(s) 204, 1394
PspN4I GGNNCC 3 cut(s) 286, 415, 1042
PspPI GGNCC 3 cut(s) 223, 413, 1311
PspPPI RGGWCCY 1 cut(s) 413
PspXI VCTCGAGB 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 1565
PvuI CGATCG 1 cut(s) 613
RsaI GTAC 4 cut(s) 435, 657, 965, 1514
RsaNI GTAC 4 cut(s) 434, 656, 964, 1513
RseI CAYNNNNRTG 3 cut(s) 504, 694, 1269
SaqAI TTAA 6 cut(s) 144, 717, 1062, 1106, 1353, 1481
SatI GCNGC 5 cut(s) 219, 792, 795, 827, 1101
Sau3AI GATC 5 cut(s) 508, 610, 1370, 1471, 1567
Sau96I GGNCC 3 cut(s) 223, 413, 1311
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 2 cut(s) 206, 1396
SduI GDGCHC 1 cut(s) 916
SexAI ACCWGGT 2 cut(s) 204, 1394
SfaNI GCATC 5 cut(s) 255, 794, 852, 952, 1552
Sfr274I CTCGAG 1 cut(s) 724
SfuI TTCGAA 1 cut(s) 597
SinI GGWCC 3 cut(s) 223, 413, 1311
SlaI CTCGAG 1 cut(s) 724
SmiMI CAYNNNNRTG 3 cut(s) 504, 694, 1269
SmlI CTYRAG 2 cut(s) 724, 969
SmoI CTYRAG 2 cut(s) 724, 969
Sse9I AATT 8 cut(s) 53, 78, 718, 759, 999, 1063, 1291, 1413
SsiI CCGC 3 cut(s) 221, 1101, 1133
SspI AATATT 2 cut(s) 440, 1325
SspMI CTAG 3 cut(s) 128, 996, 1380
StyD4I CCNGG 2 cut(s) 204, 1394
StyI CCWWGG 1 cut(s) 1199
TaaI ACNGT 4 cut(s) 506, 603, 1218, 1346
TaiI ACGT 5 cut(s) 1010, 1181, 1291, 1341, 1552
TaqI TCGA 9 cut(s) 525, 597, 613, 725, 918, 1047, 1285, 1320, 1461
TasI AATT 8 cut(s) 53, 78, 718, 759, 999, 1063, 1291, 1413
TatI WGTACW 2 cut(s) 433, 655
TauI GCSGC 1 cut(s) 1103
TfiI GAWTC 7 cut(s) 421, 477, 678, 1136, 1317, 1409, 1556
Tru1I TTAA 6 cut(s) 144, 717, 1062, 1106, 1353, 1481
Tru9I TTAA 6 cut(s) 144, 717, 1062, 1106, 1353, 1481
TscAI CASTG 5 cut(s) 295, 511, 580, 1021, 1093
TseFI GTSAC 1 cut(s) 1088
TseI GCWGC 4 cut(s) 218, 791, 794, 826
Tsp45I GTSAC 1 cut(s) 1088
TspDTI ATGAA 8 cut(s) 12, 64, 650, 752, 992, 1042, 1281, 1449
TspRI CASTG 5 cut(s) 295, 511, 580, 1021, 1093
Van91I CCANNNNNTGG 1 cut(s) 384
VpaK11BI GGWCC 3 cut(s) 223, 413, 1311
XapI RAATTY 1 cut(s) 999
XbaI TCTAGA 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 724
XspI CTAG 3 cut(s) 128, 996, 1380
Zsp2I ATGCAT 3 cut(s) 282, 787, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.