Rh5DG290100

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000569
Physical Location & Seq
Forward (+)
36216136 .. 36221880
5745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG290100.1

Sequence Viewer

Length: 1377 bp
ATGTTGAGAATGGTTCTGCAACTGGAACTGCTCCTCTTTCTTTGCTTCATAATTCTTATCAATACCCCCCTAAATCTTTCATTAGCTTTTGATTTAGAACTGGGTGCTGATGAGTTTAAAAGAGATGACTTCCCTCCCCCACCGGGCTTTGTTTTTGGTGCTTCCTCCTCTGCTTATCAGGTGGAAGGTGCTGCGGACCAAGACGGGAGAACCCCAAGCATCTTGGACACCTATGCGCATGCTGGGAAAATGCACGGAGCCACCGGAGATGTAGCGTGTGATGAGTATCATAAATACAAGGAAGATGTGCAACTAATGGTGGATACTGGTTTAGAGGCGTATAGATTTTCCATCTCATGGTCAAGACTTATACCCAATGGAAGAGGACCTGTGAATCCAAAGGGTCTACAATATTACAACAATCTAATTGACCAACTAATCAGCAAAGGAATCCAGCCTCATGTTACTTTACACCACAGTGATCTCCCACAGGCACTCGAGGATGAGTATGGAGGATGGGTTAGCCCACAGATTGTAAAAGACTTCACTGCATATGCAAATGTTTGCTTCGAGCAGTTTAGCGATCGCGTTAGGCATTGGACTACTATGAATGAGCCAAATGTATTTGTACTCGGGGGTTATGATATTGGATTCCTTCCACCGTCACGATGTTCAGCTCCATTTGGTTTTAATTGCTCCAAGGGTAACTCCTCAACTGAACCCTACATGGCCATTCATAATTTCTTGCTATCTCATGCATCAGCTGCTGCACTCTACAAGCAAACCTACCAGCACAAGCAGCGTGGGTTTATAGGCATCAATGTATTTGCCTATTGGTTTATTCCTCGAACCGAAACCCATCAAGACAAAGTTGCTGCTCAAAGAGCTATGGACTTCTACTTTGGTTGGATTATGAATCCTTTGGTGTTCGGAGAGTACCCTCAAGTAATGAAAGAGAATGCAGGCTCTAGACTTCCGACGTTCACCAGTGCTGAGTCTTCAAAGGTCAAAGGCTCGTTGGACTTCATCGGACTCAATTACTATAACACATTGTATGCCAAGGACAACTCTGCCGCCCTAAACATTCCACAAAGAGACTATATGGCTGATTCGGCAATCCAGATTTCTCCTTTGGATCACAATACATCAACGTTTGAGTATCCGATGACACCTTGGGGTATGGAAGAACTGTTGGAATATATCAAACAAAACTACGGCAATCCTCCCTTATATATCCATGAAAATGGTCAACGAACTCGACGCAATTCATCGTTGGAGGATTGGTCCAGAATCGAATATTTGCATGGACACATTCACAGTTTGCTTACTGTTTTAAGGTATGCAGCTATCTACAAGGCTACAACCATCTCTAACTAG

Protein Analysis

458

Amino Acids

51.9

Weight (kDa)

5.72

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 47 - 443 4.9e-117 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 357
AccI GTMKAC 1 cut(s) 406
AccII CGCG 1 cut(s) 588
AciI CCGC 2 cut(s) 194, 1074
AclI AACGTT 1 cut(s) 1151
AclWI GGATC 1 cut(s) 1143
AcoI YGGCCR 1 cut(s) 729
AfaI GTAC 2 cut(s) 630, 938
AfiI CCNNNNNNNGG 2 cut(s) 143, 357
AgsI TTSAA 1 cut(s) 1002
AleI CACNNNNGTG 1 cut(s) 477
AluBI AGCT 5 cut(s) 86, 677, 764, 887, 1346
AluI AGCT 5 cut(s) 86, 677, 764, 887, 1346
Alw26I GTCTC 1 cut(s) 1089
AlwI GGATC 1 cut(s) 1143
AlwNI CAGNNNCTG 1 cut(s) 767
Ama87I CYCGRG 2 cut(s) 497, 632
AoxI GGCC 1 cut(s) 729
ApeKI GCWGC 6 cut(s) 191, 764, 767, 799, 875, 1343
ArsI GACNNNNNNTTYG 2 cut(s) 884, 916
AsiSI GCGATCGC 1 cut(s) 586
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 3 cut(s) 196, 386, 1284
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 976
AvaI CYCGRG 2 cut(s) 497, 632
AvaII GGWCC 3 cut(s) 196, 386, 1284
BalI TGGCCA 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 990
BbvI GCAGC 6 cut(s) 178, 751, 754, 811, 862, 1355
BccI CCATC 4 cut(s) 359, 510, 867, 1373
BceAI ACGGC 1 cut(s) 1231
BciVI GTATCC 2 cut(s) 316, 1170
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 1089
BfaI CTAG 2 cut(s) 969, 1375
BfuI GTATCC 2 cut(s) 316, 1170
BisI GCNGC 7 cut(s) 192, 765, 768, 800, 876, 1074, 1344
BlsI GCNGC 7 cut(s) 193, 766, 769, 801, 877, 1075, 1345
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 3 cut(s) 196, 386, 1284
BmeT110I CYCGRG 2 cut(s) 497, 632
BmgT120I GGNCC 3 cut(s) 196, 386, 1284
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 3 cut(s) 228, 767, 825
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 990
BpuEI CTTGAG 1 cut(s) 927
BpuMI CCSGG 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 699, 1059, 1172
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 4 cut(s) 504, 534, 1268, 1298
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 357
Bse1I ACTGG 4 cut(s) 27, 105, 331, 987
Bse8I GATNNNNATC 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 699, 1059, 1172
BseGI GGATG 2 cut(s) 508, 521
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 143, 357
BseMII CTCAG 1 cut(s) 984
BseNI ACTGG 4 cut(s) 27, 105, 331, 987
BseRI GAGGAG 3 cut(s) 23, 157, 700
BseXI GCAGC 6 cut(s) 178, 751, 754, 811, 862, 1355
BseYI CCCAGC 1 cut(s) 242
BsgI GTGCAG 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 588
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 1 cut(s) 731
BsiEI CGRYCG 1 cut(s) 586
BsiHKCI CYCGRG 2 cut(s) 497, 632
BsiSI CCGG 2 cut(s) 143, 264
BslI CCNNNNNNNGG 2 cut(s) 143, 357
BsmAI GTCTC 1 cut(s) 1089
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 1 cut(s) 731
BsoBI CYCGRG 2 cut(s) 497, 632
Bsp143I GATC 3 cut(s) 481, 583, 1135
BspACI CCGC 2 cut(s) 194, 1074
BspANI GGCC 1 cut(s) 731
BspCNI CTCAG 1 cut(s) 985
BspFNI CGCG 1 cut(s) 588
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 1 cut(s) 1143
BsrI ACTGG 4 cut(s) 27, 105, 331, 987
BssECI CCNNGG 3 cut(s) 699, 1059, 1172
BssMI GATC 3 cut(s) 481, 583, 1135
BssT1I CCWWGG 3 cut(s) 699, 1059, 1172
Bst4CI ACNGT 5 cut(s) 479, 663, 1191, 1319, 1330
Bst6I CTCTTC 1 cut(s) 376
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 2 cut(s) 240, 964
BstDEI CTNAG 1 cut(s) 993
BstF5I GGATG 2 cut(s) 508, 521
BstFNI CGCG 1 cut(s) 588
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 3 cut(s) 484, 586, 1138
BstMAI GTCTC 1 cut(s) 1089
BstMBI GATC 3 cut(s) 481, 583, 1135
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 4 cut(s) 764, 799, 884, 1112
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 6 cut(s) 178, 751, 754, 811, 862, 1355
BstV2I GAAGAC 1 cut(s) 990
BstXI CCANNNNNNTGG 1 cut(s) 1244
BsuI GTATCC 2 cut(s) 316, 1170
BsuRI GGCC 1 cut(s) 731
BtsCI GGATG 2 cut(s) 508, 521
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 3 cut(s) 484, 546, 994
Cac8I GCNNGC 2 cut(s) 240, 964
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 3 cut(s) 196, 386, 1284
CseI GACGC 1 cut(s) 1269
Csp6I GTAC 2 cut(s) 629, 937
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 7 cut(s) 239, 357, 461, 727, 755, 1238, 1304
CviQI GTAC 2 cut(s) 629, 937
DdeI CTNAG 1 cut(s) 993
DpnI GATC 3 cut(s) 483, 585, 1137
DpnII GATC 3 cut(s) 481, 583, 1135
DraI TTTAAA 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 729
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco130I CCWWGG 3 cut(s) 699, 1059, 1172
Eco47I GGWCC 3 cut(s) 196, 386, 1284
Eco88I CYCGRG 2 cut(s) 497, 632
EcoO109I RGGNCCY 1 cut(s) 386
EcoT14I CCWWGG 3 cut(s) 699, 1059, 1172
EcoT22I ATGCAT 1 cut(s) 760
ErhI CCWWGG 3 cut(s) 699, 1059, 1172
FaeI CATG 7 cut(s) 242, 360, 464, 730, 758, 1241, 1307
FatI CATG 7 cut(s) 238, 356, 460, 726, 754, 1237, 1303
FauNDI CATATG 1 cut(s) 553
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 7 cut(s) 192, 765, 768, 800, 876, 1074, 1344
FokI GGATG 2 cut(s) 515, 528
Fsp4HI GCNGC 7 cut(s) 192, 765, 768, 800, 876, 1074, 1344
FspAI RTGCGCAY 1 cut(s) 237
FspBI CTAG 2 cut(s) 969, 1375
FspI TGCGCA 1 cut(s) 237
GlaI GCGC 1 cut(s) 237
GluI GCNGC 7 cut(s) 192, 765, 768, 800, 876, 1074, 1344
GsaI CCCAGC 1 cut(s) 246
HaeIII GGCC 1 cut(s) 731
HapII CCGG 2 cut(s) 143, 264
HgaI GACGC 1 cut(s) 1269
HhaI GCGC 1 cut(s) 238
Hin1II CATG 7 cut(s) 242, 360, 464, 730, 758, 1241, 1307
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HincII GTYRAC 1 cut(s) 1250
HindII GTYRAC 1 cut(s) 1250
HinfI GANTC 8 cut(s) 394, 450, 651, 916, 995, 1032, 1109, 1290
HpaII CCGG 2 cut(s) 143, 264
HphI GGTGA 1 cut(s) 976
Hpy166II GTNNAC 3 cut(s) 407, 984, 1250
Hpy188I TCNGA 4 cut(s) 932, 978, 1031, 1164
Hpy188III TCNNGA 6 cut(s) 363, 666, 863, 969, 1120, 1287
Hpy8I GTNNAC 3 cut(s) 407, 984, 1250
Hpy99I CGWCG 2 cut(s) 982, 1263
HpyAV CCTTC 2 cut(s) 179, 665
HpyCH4III ACNGT 5 cut(s) 479, 663, 1191, 1319, 1330
HpyCH4IV ACGT 2 cut(s) 980, 1151
HpyF10VI GCNNNNNNNGC 4 cut(s) 764, 799, 884, 1112
HpyF3I CTNAG 1 cut(s) 993
HpySE526I ACGT 2 cut(s) 980, 1151
Hsp92II CATG 7 cut(s) 242, 360, 464, 730, 758, 1241, 1307
HspAI GCGC 1 cut(s) 236
Kzo9I GATC 3 cut(s) 481, 583, 1135
LmnI GCTCC 4 cut(s) 36, 257, 682, 701
Lsp1109I GCAGC 6 cut(s) 178, 751, 754, 811, 862, 1355
LweI GCATC 3 cut(s) 228, 767, 825
MaeI CTAG 2 cut(s) 969, 1375
MaeII ACGT 2 cut(s) 980, 1151
MaeIII GTNAC 3 cut(s) 463, 663, 704
MalI GATC 3 cut(s) 483, 585, 1137
MboI GATC 3 cut(s) 481, 583, 1135
MboII GAAGA 4 cut(s) 314, 393, 990, 1196
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 6 cut(s) 51, 426, 691, 739, 1036, 1264
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 2 cut(s) 1004, 1026
MmeI TCCRAC 5 cut(s) 887, 999, 1001, 1173, 1254
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 1 cut(s) 760
MscI TGGCCA 1 cut(s) 731
MseI TTAA 3 cut(s) 117, 690, 1334
MslI CAYNNNNRTG 2 cut(s) 477, 1242
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 2 cut(s) 143, 264
MspR9I CCNGG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 964
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 4 cut(s) 764, 799, 884, 1112
NciI CCSGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 553
NdeII GATC 3 cut(s) 481, 583, 1135
NlaIII CATG 7 cut(s) 242, 360, 464, 730, 758, 1241, 1307
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 663
NsbI TGCGCA 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 1 cut(s) 242
OliI CACNNNNGTG 1 cut(s) 477
PaeI GCATGC 1 cut(s) 242
PaeR7I CTCGAG 1 cut(s) 497
PctI GAATGC 1 cut(s) 964
PfeI GAWTC 6 cut(s) 394, 450, 651, 916, 1109, 1290
PflMI CCANNNNNTGG 1 cut(s) 357
PkrI GCNGC 7 cut(s) 193, 766, 769, 801, 877, 1075, 1345
Ple19I CGATCG 1 cut(s) 586
PleI GAGTC 2 cut(s) 1003, 1026
PpsI GAGTC 2 cut(s) 1003, 1026
PpuMI RGGWCCY 1 cut(s) 386
Psp1406I AACGTT 1 cut(s) 1151
Psp5II RGGWCCY 1 cut(s) 386
PspFI CCCAGC 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 3 cut(s) 196, 386, 1284
PspPPI RGGWCCY 1 cut(s) 386
PspXI VCTCGAGB 1 cut(s) 497
PstNI CAGNNNCTG 1 cut(s) 767
PvuI CGATCG 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 764
RgaI GCGATCGC 1 cut(s) 586
RsaI GTAC 2 cut(s) 630, 938
RsaNI GTAC 2 cut(s) 629, 937
RseI CAYNNNNRTG 2 cut(s) 477, 1242
SaqAI TTAA 3 cut(s) 117, 690, 1334
SatI GCNGC 7 cut(s) 192, 765, 768, 800, 876, 1074, 1344
Sau3AI GATC 3 cut(s) 481, 583, 1135
Sau96I GGNCC 3 cut(s) 196, 386, 1284
SchI GAGTC 2 cut(s) 1004, 1026
ScrFI CCNGG 1 cut(s) 144
SfaAI GCGATCGC 1 cut(s) 586
SfaNI GCATC 3 cut(s) 228, 767, 825
Sfr274I CTCGAG 1 cut(s) 497
SgfI GCGATCGC 1 cut(s) 586
SinI GGWCC 3 cut(s) 196, 386, 1284
SlaI CTCGAG 1 cut(s) 497
SmiMI CAYNNNNRTG 2 cut(s) 477, 1242
SmlI CTYRAG 2 cut(s) 497, 942
SmoI CTYRAG 2 cut(s) 497, 942
SphI GCATGC 1 cut(s) 242
Sse9I AATT 6 cut(s) 51, 426, 691, 739, 1036, 1264
SsiI CCGC 2 cut(s) 194, 1074
SspI AATATT 2 cut(s) 413, 1298
SspMI CTAG 2 cut(s) 969, 1375
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 3 cut(s) 699, 1059, 1172
TaaI ACNGT 5 cut(s) 479, 663, 1191, 1319, 1330
TaiI ACGT 2 cut(s) 983, 1154
TaqI TCGA 5 cut(s) 498, 570, 847, 1258, 1293
TasI AATT 6 cut(s) 51, 426, 691, 739, 1036, 1264
TatI WGTACW 1 cut(s) 628
TauI GCSGC 1 cut(s) 1076
TfiI GAWTC 6 cut(s) 394, 450, 651, 916, 1109, 1290
Tru1I TTAA 3 cut(s) 117, 690, 1334
Tru9I TTAA 3 cut(s) 117, 690, 1334
TscAI CASTG 3 cut(s) 484, 553, 994
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 6 cut(s) 191, 764, 767, 799, 875, 1343
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 9 cut(s) 37, 69, 623, 725, 929, 965, 1015, 1254, 1257
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 3 cut(s) 484, 553, 994
Van91I CCANNNNNTGG 1 cut(s) 357
VpaK11BI GGWCC 3 cut(s) 196, 386, 1284
XbaI TCTAGA 1 cut(s) 968
XceI RCATGY 1 cut(s) 242
XhoI CTCGAG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 406
XspI CTAG 2 cut(s) 969, 1375
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.