RchiOBHm_Chr5g0041231

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000569
Physical Location & Seq
Forward (+)
35948379 .. 35954215
5837 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31967

Sequence Viewer

Length: 1467 bp
ATGAGTTTAAAAGAGATGACTTCCCTCCCCCACCGGGCTTTGTTTTTGGTGCTTCCTCCTCTGCTTATCAGGTACGACCATCCGGTGGAAGGTGCTGCGGACCAAGACGGGAGAACCCCAAGCATCTTGGACACCTATGCGCATGCTGGGAAAATGCACGGAGCCACCGGAGATGTAGCGTGTGATGAGTATCATAAATACAAGGAAGATGTGCAACTAATGGTGGATACTGGTTTAGAGGCGTATAGATTTTCCATCTCATGGTCAAGACTTATACCCAATGGAAGAGGACCTGTGAATCCAAAGGGTCTACAATATTACAACAATCTAATTGACCAACTAATCAGCAAAGGAATCCAGCCTCATGTTACTTTACACCACAGTGATCTCCCACAGGCACTCGAGGATGAGTATGGAGGATGGGTTAGCCCACAGATTGTAAAAGACTTCACTGCATATGCAAATGTTTGCTTCGAACAGTTTGGCGATCGTGTTAGGCATTGGACTACTATGAATGAGCCAAATGTATTTGTACTCGGGGGTTATGATATTGGATTCCTTCCACCGTCACGATGTTCAGCTCCATTTGGTTTTAATTGCTCCAAGGGTAACTCCTCAACTGAACCCTACATGGCCATTCATAATTTCTTGCTATCTCATGCATCAGCTGCTGCACTCTACAAGCAAACCTACCAGCACAAGCAGCGTGGGTTTATAGGCATCAATGTATTTGCCTATTGGTTTATTCCTCGAACCGAAACCCATCAAGACAAAGTTGCTGCTCAAAGAGCTTTGGACTTCTACTTTGGTTGGATTATGAATCCTTTGGTGTTCGGAGAGTACCCTCAAGTAATGAAAGAGAATGCAGGCTCTAGGCTTCCGACGTTCACCAGTGCTGAGTCTTCAAAGGTCAAAGGCTCGTTGGACTTCATCGGACTGAATTACTATAACACATTGTATGCCAAGGACAACTCTGCCGCCCTAAACATTCCAAAAAGAGACTATATGGCTGATTCGGCAATCCAGATTTCTCCTTTGGATCTCAATACATCAACGTTTGAGTATCCGATAACACCTTGGGGTATGGAAGAACTGTTGGAATATATCAAACAAAACTACGGCAATCCTCCCTTATATATCCATGAAAATGGTCAACGAACTCGACGCAATTCATCGTTGGAGGATTGGTCCAGAATCGAATATTTGCATGGACACATTCACAGTTTGCTTAATGTTTTAAGGAATGGGTCAAATGCCAGAGGCTATTTTACCTGGTCTCTCTTGGATTCACTTGAGCTTTTGGATGGCTATGAATCAAGCTACGGCCTTTACTACATCGATTTGGACGATCCTGATTTAAAAAGACAGCCTAAACTTTCTGCGCATTGGTACTCAAAGTTTCTGAAGAACAAAAACTTCACTCCACGTTCAGTTTCAGATGCTGATCAACATTACTTCTTTCAGTAG

Protein Analysis

488

Amino Acids

55.78

Weight (kDa)

6.09

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 29 - 472 2.2e-132 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 141, 1383
AccB7I CCANNNNNTGG 2 cut(s) 85, 261
AccI GTMKAC 1 cut(s) 310
AciI CCGC 2 cut(s) 98, 978
AclI AACGTT 1 cut(s) 1055
AclWI GGATC 2 cut(s) 1047, 1343
AcoI YGGCCR 1 cut(s) 633
AcuI CTGAAG 1 cut(s) 1424
AfaI GTAC 4 cut(s) 74, 534, 842, 1391
AfiI CCNNNNNNNGG 4 cut(s) 34, 85, 89, 261
AgsI TTSAA 1 cut(s) 906
AjnI CCWGG 1 cut(s) 1271
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 5 cut(s) 581, 668, 791, 1297, 1320
AluI AGCT 5 cut(s) 581, 668, 791, 1297, 1320
Alw26I GTCTC 2 cut(s) 993, 1281
AlwI GGATC 2 cut(s) 1047, 1343
AlwNI CAGNNNCTG 2 cut(s) 671, 1442
Ama87I CYCGRG 2 cut(s) 401, 536
AoxI GGCC 2 cut(s) 633, 1324
ApeKI GCWGC 5 cut(s) 95, 668, 671, 703, 779
ArsI GACNNNNNNTTYG 2 cut(s) 788, 820
AspLEI GCGC 2 cut(s) 142, 1384
AspS9I GGNCC 3 cut(s) 100, 290, 1188
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 880
AsuII TTCGAA 1 cut(s) 474
AvaI CYCGRG 2 cut(s) 401, 536
AvaII GGWCC 3 cut(s) 100, 290, 1188
BalI TGGCCA 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 894
BbvI GCAGC 5 cut(s) 82, 655, 658, 715, 766
BccI CCATC 5 cut(s) 87, 263, 414, 771, 1298
BceAI ACGGC 2 cut(s) 1135, 1339
BciT130I CCWGG 1 cut(s) 1273
BciVI GTATCC 2 cut(s) 220, 1074
BclI TGATCA 1 cut(s) 1444
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 993, 1281
BfaI CTAG 1 cut(s) 873
BfuI GTATCC 2 cut(s) 220, 1074
BisI GCNGC 6 cut(s) 96, 669, 672, 704, 780, 978
BlsI GCNGC 6 cut(s) 97, 670, 673, 705, 781, 979
Bme1390I CCNGG 2 cut(s) 35, 1273
Bme18I GGWCC 3 cut(s) 100, 290, 1188
BmeT110I CYCGRG 2 cut(s) 401, 536
BmgT120I GGNCC 3 cut(s) 100, 290, 1188
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 35, 1273
BmsI GCATC 4 cut(s) 132, 671, 729, 1429
BpiI GAAGAC 1 cut(s) 894
Bpu14I TTCGAA 1 cut(s) 474
BpuEI CTTGAG 2 cut(s) 831, 1313
BpuMI CCSGG 1 cut(s) 35
Bsa29I ATCGAT 1 cut(s) 1338
BsaBI GATNNNNATC 2 cut(s) 189, 1443
BsaI GGTCTC 1 cut(s) 1281
BsaJI CCNNGG 3 cut(s) 603, 963, 1076
BsaWI WCCGGW 2 cut(s) 82, 167
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 1172, 1202
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 85, 89, 261
Bse1I ACTGG 2 cut(s) 235, 891
Bse8I GATNNNNATC 2 cut(s) 189, 1443
BseBI CCWGG 1 cut(s) 1273
BseCI ATCGAT 1 cut(s) 1338
BseDI CCNNGG 3 cut(s) 603, 963, 1076
BseGI GGATG 4 cut(s) 79, 412, 425, 1309
BseJI GATNNNNATC 2 cut(s) 189, 1443
BseLI CCNNNNNNNGG 4 cut(s) 34, 85, 89, 261
BseMII CTCAG 1 cut(s) 888
BseNI ACTGG 2 cut(s) 235, 891
BseRI GAGGAG 2 cut(s) 48, 604
BseXI GCAGC 5 cut(s) 82, 655, 658, 715, 766
BseYI CCCAGC 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 657
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 2 cut(s) 635, 1326
BshVI ATCGAT 1 cut(s) 1338
BsiEI CGRYCG 1 cut(s) 490
BsiHKCI CYCGRG 2 cut(s) 401, 536
BsiSI CCGG 3 cut(s) 34, 83, 168
BslI CCNNNNNNNGG 4 cut(s) 34, 85, 89, 261
BsmAI GTCTC 2 cut(s) 993, 1281
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 2 cut(s) 635, 1326
Bso31I GGTCTC 1 cut(s) 1281
BsoBI CYCGRG 2 cut(s) 401, 536
Bsp119I TTCGAA 1 cut(s) 474
Bsp143I GATC 5 cut(s) 385, 487, 1039, 1348, 1444
BspACI CCGC 2 cut(s) 98, 978
BspANI GGCC 2 cut(s) 635, 1326
BspCNI CTCAG 1 cut(s) 889
BspDI ATCGAT 1 cut(s) 1338
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 2 cut(s) 1047, 1343
BspT104I TTCGAA 1 cut(s) 474
BspTNI GGTCTC 1 cut(s) 1281
BsrI ACTGG 2 cut(s) 235, 891
BssECI CCNNGG 3 cut(s) 603, 963, 1076
BssMI GATC 5 cut(s) 385, 487, 1039, 1348, 1444
BssT1I CCWWGG 3 cut(s) 603, 963, 1076
Bst2UI CCWGG 1 cut(s) 1273
Bst4CI ACNGT 5 cut(s) 383, 480, 567, 1095, 1223
Bst6I CTCTTC 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 144, 868
BstDEI CTNAG 1 cut(s) 897
BstF5I GGATG 4 cut(s) 79, 412, 425, 1309
BstHHI GCGC 2 cut(s) 142, 1384
BstKTI GATC 5 cut(s) 388, 490, 1042, 1351, 1447
BstMAI GTCTC 2 cut(s) 993, 1281
BstMBI GATC 5 cut(s) 385, 487, 1039, 1348, 1444
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 4 cut(s) 668, 703, 788, 1016
BstNI CCWGG 1 cut(s) 1273
BstNSI RCATGY 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 33, 1271
BstV1I GCAGC 5 cut(s) 82, 655, 658, 715, 766
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 1 cut(s) 1039
BstXI CCANNNNNNTGG 1 cut(s) 1148
BstYI RGATCY 1 cut(s) 1039
Bsu15I ATCGAT 1 cut(s) 1338
BsuI GTATCC 2 cut(s) 220, 1074
BsuRI GGCC 2 cut(s) 635, 1326
BsuTUI ATCGAT 1 cut(s) 1338
BtsCI GGATG 4 cut(s) 79, 412, 425, 1309
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 3 cut(s) 388, 450, 898
Cac8I GCNNGC 2 cut(s) 144, 868
CaiI CAGNNNCTG 2 cut(s) 671, 1442
CfoI GCGC 2 cut(s) 142, 1384
Cfr13I GGNCC 3 cut(s) 100, 290, 1188
ClaI ATCGAT 1 cut(s) 1338
CseI GACGC 1 cut(s) 1173
CsiI ACCWGGT 1 cut(s) 1271
Csp6I GTAC 4 cut(s) 73, 533, 841, 1390
CspCI CAANNNNNGTGG 2 cut(s) 688, 723
CviAII CATG 7 cut(s) 143, 261, 365, 631, 659, 1142, 1208
CviQI GTAC 4 cut(s) 73, 533, 841, 1390
DdeI CTNAG 1 cut(s) 897
DpnI GATC 5 cut(s) 387, 489, 1041, 1350, 1446
DpnII GATC 5 cut(s) 385, 487, 1039, 1348, 1444
DraI TTTAAA 2 cut(s) 9, 1359
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco130I CCWWGG 3 cut(s) 603, 963, 1076
Eco31I GGTCTC 1 cut(s) 1281
Eco47I GGWCC 3 cut(s) 100, 290, 1188
Eco57I CTGAAG 1 cut(s) 1424
Eco88I CYCGRG 2 cut(s) 401, 536
EcoO109I RGGNCCY 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 1271
EcoT14I CCWWGG 3 cut(s) 603, 963, 1076
EcoT22I ATGCAT 1 cut(s) 664
ErhI CCWWGG 3 cut(s) 603, 963, 1076
FaeI CATG 7 cut(s) 146, 264, 368, 634, 662, 1145, 1211
FatI CATG 7 cut(s) 142, 260, 364, 630, 658, 1141, 1207
FauNDI CATATG 1 cut(s) 457
FbaI TGATCA 1 cut(s) 1444
FblI GTMKAC 1 cut(s) 310
Fnu4HI GCNGC 6 cut(s) 96, 669, 672, 704, 780, 978
FokI GGATG 4 cut(s) 66, 419, 432, 1316
Fsp4HI GCNGC 6 cut(s) 96, 669, 672, 704, 780, 978
FspAI RTGCGCAY 1 cut(s) 141
FspBI CTAG 1 cut(s) 873
FspI TGCGCA 2 cut(s) 141, 1383
GlaI GCGC 2 cut(s) 141, 1383
GluI GCNGC 6 cut(s) 96, 669, 672, 704, 780, 978
GsaI CCCAGC 1 cut(s) 150
HaeIII GGCC 2 cut(s) 635, 1326
HapII CCGG 3 cut(s) 34, 83, 168
HgaI GACGC 1 cut(s) 1173
HhaI GCGC 2 cut(s) 142, 1384
Hin1II CATG 7 cut(s) 146, 264, 368, 634, 662, 1145, 1211
Hin6I GCGC 2 cut(s) 140, 1382
HinP1I GCGC 2 cut(s) 140, 1382
HincII GTYRAC 1 cut(s) 1154
HindII GTYRAC 1 cut(s) 1154
HinfI GANTC 9 cut(s) 298, 354, 555, 820, 899, 1013, 1194, 1286, 1313
HpaII CCGG 3 cut(s) 34, 83, 168
HphI GGTGA 1 cut(s) 880
Hpy166II GTNNAC 3 cut(s) 311, 888, 1154
Hpy188I TCNGA 6 cut(s) 836, 882, 935, 1068, 1404, 1438
Hpy188III TCNNGA 6 cut(s) 267, 570, 767, 1024, 1191, 1352
Hpy8I GTNNAC 3 cut(s) 311, 888, 1154
Hpy99I CGWCG 2 cut(s) 886, 1167
HpyAV CCTTC 2 cut(s) 83, 569
HpyCH4III ACNGT 5 cut(s) 383, 480, 567, 1095, 1223
HpyCH4IV ACGT 3 cut(s) 884, 1055, 1426
HpyCH4V TGCA 8 cut(s) 157, 214, 455, 461, 662, 674, 866, 1207
HpyF10VI GCNNNNNNNGC 4 cut(s) 668, 703, 788, 1016
HpyF3I CTNAG 1 cut(s) 897
HpySE526I ACGT 3 cut(s) 884, 1055, 1426
Hsp92II CATG 7 cut(s) 146, 264, 368, 634, 662, 1145, 1211
HspAI GCGC 2 cut(s) 140, 1382
Ksp22I TGATCA 1 cut(s) 1444
Kzo9I GATC 5 cut(s) 385, 487, 1039, 1348, 1444
LmnI GCTCC 3 cut(s) 161, 586, 605
Lsp1109I GCAGC 5 cut(s) 82, 655, 658, 715, 766
LweI GCATC 4 cut(s) 132, 671, 729, 1429
MabI ACCWGGT 1 cut(s) 1271
MaeI CTAG 1 cut(s) 873
MaeII ACGT 3 cut(s) 884, 1055, 1426
MaeIII GTNAC 3 cut(s) 367, 567, 608
MalI GATC 5 cut(s) 387, 489, 1041, 1350, 1446
MboI GATC 5 cut(s) 385, 487, 1039, 1348, 1444
MboII GAAGA 5 cut(s) 218, 297, 894, 1100, 1417
MflI RGATCY 1 cut(s) 1039
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 5 cut(s) 330, 595, 643, 940, 1168
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 1 cut(s) 908
MmeI TCCRAC 5 cut(s) 791, 903, 905, 1077, 1158
Mox20I TGGCCA 1 cut(s) 635
Mph1103I ATGCAT 1 cut(s) 664
MscI TGGCCA 1 cut(s) 635
MseI TTAA 5 cut(s) 8, 594, 1230, 1238, 1358
MslI CAYNNNNRTG 2 cut(s) 381, 1146
Msp20I TGGCCA 1 cut(s) 635
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 3 cut(s) 34, 83, 168
MspR9I CCNGG 2 cut(s) 35, 1273
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 1 cut(s) 1273
MwoI GCNNNNNNNGC 4 cut(s) 668, 703, 788, 1016
NciI CCSGG 1 cut(s) 35
NdeI CATATG 1 cut(s) 457
NdeII GATC 5 cut(s) 385, 487, 1039, 1348, 1444
NlaIII CATG 7 cut(s) 146, 264, 368, 634, 662, 1145, 1211
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 567
NsbI TGCGCA 2 cut(s) 141, 1383
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 146
NspV TTCGAA 1 cut(s) 474
OliI CACNNNNGTG 1 cut(s) 381
PaeI GCATGC 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 1344
PctI GAATGC 1 cut(s) 868
PfeI GAWTC 8 cut(s) 298, 354, 555, 820, 1013, 1194, 1286, 1313
PflMI CCANNNNNTGG 2 cut(s) 85, 261
PkrI GCNGC 6 cut(s) 97, 670, 673, 705, 781, 979
Ple19I CGATCG 1 cut(s) 490
PleI GAGTC 1 cut(s) 907
PpsI GAGTC 1 cut(s) 907
PpuMI RGGWCCY 1 cut(s) 290
Psp1406I AACGTT 1 cut(s) 1055
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 1271
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 1271
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 3 cut(s) 100, 290, 1188
PspPPI RGGWCCY 1 cut(s) 290
PspXI VCTCGAGB 1 cut(s) 401
PstNI CAGNNNCTG 2 cut(s) 671, 1442
PsuI RGATCY 1 cut(s) 1039
PvuI CGATCG 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 4 cut(s) 74, 534, 842, 1391
RsaNI GTAC 4 cut(s) 73, 533, 841, 1390
RseI CAYNNNNRTG 2 cut(s) 381, 1146
SaqAI TTAA 5 cut(s) 8, 594, 1230, 1238, 1358
SatI GCNGC 6 cut(s) 96, 669, 672, 704, 780, 978
Sau3AI GATC 5 cut(s) 385, 487, 1039, 1348, 1444
Sau96I GGNCC 3 cut(s) 100, 290, 1188
SchI GAGTC 1 cut(s) 908
ScrFI CCNGG 2 cut(s) 35, 1273
SexAI ACCWGGT 1 cut(s) 1271
SfaNI GCATC 4 cut(s) 132, 671, 729, 1429
Sfr274I CTCGAG 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 474
SinI GGWCC 3 cut(s) 100, 290, 1188
SlaI CTCGAG 1 cut(s) 401
SmiMI CAYNNNNRTG 2 cut(s) 381, 1146
SmlI CTYRAG 3 cut(s) 401, 846, 1292
SmoI CTYRAG 3 cut(s) 401, 846, 1292
SphI GCATGC 1 cut(s) 146
Sse9I AATT 5 cut(s) 330, 595, 643, 940, 1168
SsiI CCGC 2 cut(s) 98, 978
SspI AATATT 2 cut(s) 317, 1202
SspMI CTAG 1 cut(s) 873
StyD4I CCNGG 2 cut(s) 33, 1271
StyI CCWWGG 3 cut(s) 603, 963, 1076
TaaI ACNGT 5 cut(s) 383, 480, 567, 1095, 1223
TaiI ACGT 3 cut(s) 887, 1058, 1429
TaqI TCGA 6 cut(s) 402, 474, 751, 1162, 1197, 1338
TasI AATT 5 cut(s) 330, 595, 643, 940, 1168
TatI WGTACW 1 cut(s) 532
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 8 cut(s) 298, 354, 555, 820, 1013, 1194, 1286, 1313
Tru1I TTAA 5 cut(s) 8, 594, 1230, 1238, 1358
Tru9I TTAA 5 cut(s) 8, 594, 1230, 1238, 1358
TscAI CASTG 3 cut(s) 388, 457, 898
TseFI GTSAC 1 cut(s) 567
TseI GCWGC 5 cut(s) 95, 668, 671, 703, 779
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 8 cut(s) 527, 629, 833, 869, 919, 1158, 1161, 1326
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 3 cut(s) 388, 457, 898
Van91I CCANNNNNTGG 2 cut(s) 85, 261
VpaK11BI GGWCC 3 cut(s) 100, 290, 1188
XceI RCATGY 1 cut(s) 146
XhoI CTCGAG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 1 cut(s) 873
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.