Rh5AG277000

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000569
Physical Location & Seq
Forward (+)
37189601 .. 37218271
28671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG277000.1

Sequence Viewer

Length: 1428 bp
ATGGTTCTGCAACTGGAACTGCTCCTCTTTCTTTGCATCATAATTCTTATCAGTACCCCCCTAAATCTTTCCTTAGCTTCTGATTTAGAACTAGGCGCTGATGATGATGAGTTTAAAAGGGACGACTTTCCTCCCCCACCGGACTTTGTTTTTGGTGCTTCCTCCTCTGCTTATCAGGTGGAAGGTGCTGCAGACCAAGACGGGAGAACCCCAAGCATCTTGGACACGTATGCGCATGCTGGTAAAATGCATGGAGCCACCGGAGATGTAGCATGTGATGAGTATCATAAATACAAGGAAGATGTGCAACTAATGGTGGATGCTGGTTTAGAGGCGTATAGATTTTCCATCTCATGGTCAAGACTCATACCCAATGGCAGAGGACCTGTGAATCCAAAGGGTCTACAATATTACAACAATCTCATTGACCAACTCATTAGCAAAGCATGCATGCGGGAATCCAGCCTCATGTTACGTACTTTACACCACAGTGATCTCCCACAGGCACTCGACGATGAGTATGGAGTATGGGTTAGCCCACAGATTGTAAAAGACTTCACTCAGTTCACTGCATATGCAAATGTTTGCTTCGAACAGTTTGGCGATCGAGTTGGGCATTGGACTACTATGAATGAGCCAAATGTATTTGTACTCGGTGGTTATGATATTGGATTCCTTCCACCACAACGGTGCTCAGGTCCATTTGGTTTTAACTGCTCGAGGGGTAACTCCACAACTGAGCCATACTTGGCAATTCATAATATCTTGTTATCTCAAGCAACAGCTGCTGCACTCTACAAGCATACCTACCAGCACAAGCAGCGTGGGTTTATAGGCATCATTCTATTTTCCCATTGGTTTATTCCTCGAACCAAAACCCATCAAGACCAAGTTGCTGCTCAAGGAGCTCTCGACTTCTATTTTGGTTGGGTTATGCATCCTTTGGTGTTTGGAGTGTACCCTCAAGTAATGAAAAAGAATGCAGGCTCTAGAATTCCAACGTTCACCCGTGCTGAGTCTGCACTGGTCAAGGGTTCCTTTGACTTCATCGGGCTCAATTACTATAACACACTGTATGCCAATAATGACGACTCTGCCGCCTTAAACATTCAGAAAAGGGACTATACAGCTGATTCGGCCATCCAAATTTCTTGTATGATAGCAGAGTTAGAAATAATATCGAAAGATGGAGAACTTGGAGGAGAAGCACCATTTTCTCAATTAGATGAGGCCATTTCAGAACTTGAGAAGGTTTCCTCTGTGACGCTTTCACAACTGCAGAATCATCCCCAAGCTGTTGATCTCAATCTCCTCTCGATTATCCAAGCTTTTCCCTTCTCCTTTATCTGGGAATTGGGAGATCAGGTACTCTTTTGTTGTGCAGAACTTTTCAGATGCGAAACTTCAAATAAAACTTTAGCTGCTTAA

Protein Analysis

475

Amino Acids

52.94

Weight (kDa)

4.9

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 46 - 373 9.9e-92 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 354
AccI GTMKAC 1 cut(s) 403
AciI CCGC 2 cut(s) 454, 1098
AclI AACGTT 1 cut(s) 1001
AcoI YGGCCR 1 cut(s) 1137
AcsI RAATTY 2 cut(s) 993, 1146
AfaI GTAC 5 cut(s) 55, 478, 651, 959, 1368
AfiI CCNNNNNNNGG 2 cut(s) 354, 1347
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 1 cut(s) 1407
AleI CACNNNNGTG 2 cut(s) 489, 688
AluBI AGCT 7 cut(s) 77, 785, 908, 1130, 1295, 1328, 1421
AluI AGCT 7 cut(s) 77, 785, 908, 1130, 1295, 1328, 1421
Alw21I GWGCWC 2 cut(s) 695, 910
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 718
AoxI GGCC 2 cut(s) 1137, 1230
ApeKI GCWGC 6 cut(s) 188, 785, 788, 820, 896, 1421
ApoI RAATTY 2 cut(s) 993, 1146
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 905, 937
AspLEI GCGC 2 cut(s) 98, 235
AspS9I GGNCC 2 cut(s) 383, 698
AsuHPI GGTGA 1 cut(s) 997
AsuII TTCGAA 1 cut(s) 591
AvaI CYCGRG 1 cut(s) 718
AvaII GGWCC 2 cut(s) 383, 698
BanII GRGCYC 2 cut(s) 910, 1056
Bbv12I GWGCWC 2 cut(s) 695, 910
BbvI GCAGC 6 cut(s) 175, 772, 775, 832, 883, 1408
BccI CCATC 4 cut(s) 356, 888, 1148, 1181
BfaI CTAG 2 cut(s) 92, 990
BfmI CTRYAG 2 cut(s) 189, 1277
BfoI RGCGCY 1 cut(s) 99
BisI GCNGC 7 cut(s) 189, 786, 789, 821, 897, 1098, 1422
BlsI GCNGC 7 cut(s) 190, 787, 790, 822, 898, 1099, 1423
Bme18I GGWCC 2 cut(s) 383, 698
BmeT110I CYCGRG 1 cut(s) 718
BmgT120I GGNCC 2 cut(s) 383, 698
BmiI GGNNCC 2 cut(s) 256, 1036
BmsI GCATC 6 cut(s) 45, 225, 310, 846, 946, 1385
Bpu10I CCTNAGC 2 cut(s) 73, 694
Bpu14I TTCGAA 1 cut(s) 591
BpuEI CTTGAG 4 cut(s) 759, 885, 948, 1265
BsaAI YACGTR 2 cut(s) 228, 476
BsaBI GATNNNNATC 2 cut(s) 282, 1305
BsaWI WCCGGW 2 cut(s) 139, 260
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 1350, 1380
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 1347
Bse1I ACTGG 2 cut(s) 18, 1029
Bse8I GATNNNNATC 2 cut(s) 282, 1305
BseGI GGATG 4 cut(s) 325, 937, 1140, 1285
BseJI GATNNNNATC 2 cut(s) 282, 1305
BseLI CCNNNNNNNGG 2 cut(s) 354, 1347
BseMII CTCAG 4 cut(s) 575, 708, 729, 1005
BseNI ACTGG 2 cut(s) 18, 1029
BseRI GAGGAG 4 cut(s) 14, 154, 1215, 1301
BseXI GCAGC 6 cut(s) 175, 772, 775, 832, 883, 1408
BsgI GTGCAG 3 cut(s) 774, 1005, 1401
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 2 cut(s) 1139, 1232
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 695, 910
BsiHKCI CYCGRG 1 cut(s) 718
BsiSI CCGG 2 cut(s) 140, 261
BslFI GGGAC 2 cut(s) 134, 1133
BslI CCNNNNNNNGG 2 cut(s) 354, 1347
BsmFI GGGAC 2 cut(s) 134, 1133
BsmI GAATGC 1 cut(s) 985
BsnI GGCC 2 cut(s) 1139, 1232
BsoBI CYCGRG 1 cut(s) 718
Bsp119I TTCGAA 1 cut(s) 591
Bsp1286I GDGCHC 3 cut(s) 695, 910, 1056
Bsp143I GATC 4 cut(s) 493, 604, 1300, 1360
BspACI CCGC 2 cut(s) 454, 1098
BspANI GGCC 2 cut(s) 1139, 1232
BspCNI CTCAG 4 cut(s) 574, 707, 730, 1006
BspLI GGNNCC 2 cut(s) 256, 1036
BspMAI CTGCAG 2 cut(s) 193, 1281
BspT104I TTCGAA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 18, 1029
BssMI GATC 4 cut(s) 493, 604, 1300, 1360
Bst4CI ACNGT 4 cut(s) 491, 597, 690, 1074
BstAPI GCANNNNNTGC 2 cut(s) 447, 785
BstBAI YACGTR 2 cut(s) 228, 476
BstBI TTCGAA 1 cut(s) 591
BstC8I GCNNGC 4 cut(s) 237, 448, 452, 985
BstDEI CTNAG 5 cut(s) 73, 561, 694, 738, 1014
BstF5I GGATG 4 cut(s) 325, 937, 1140, 1285
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 235
BstKTI GATC 4 cut(s) 496, 607, 1303, 1363
BstMBI GATC 4 cut(s) 493, 604, 1300, 1360
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 6 cut(s) 447, 785, 820, 905, 1019, 1136
BstNSI RCATGY 4 cut(s) 239, 276, 450, 454
BstSFI CTRYAG 2 cut(s) 189, 1277
BstSNI TACGTA 1 cut(s) 476
BstV1I GCAGC 6 cut(s) 175, 772, 775, 832, 883, 1408
BsuRI GGCC 2 cut(s) 1139, 1232
BtsCI GGATG 4 cut(s) 325, 937, 1140, 1285
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 4 cut(s) 496, 567, 1022, 1070
Cac8I GCNNGC 4 cut(s) 237, 448, 452, 985
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 2 cut(s) 98, 235
Cfr13I GGNCC 2 cut(s) 383, 698
CseI GACGC 1 cut(s) 1273
Csp6I GTAC 5 cut(s) 54, 477, 650, 958, 1367
CspCI CAANNNNNGTGG 2 cut(s) 805, 840
CviAII CATG 7 cut(s) 236, 251, 273, 354, 447, 451, 469
CviQI GTAC 5 cut(s) 54, 477, 650, 958, 1367
DdeI CTNAG 5 cut(s) 73, 561, 694, 738, 1014
DpnI GATC 4 cut(s) 495, 606, 1302, 1362
DpnII GATC 4 cut(s) 493, 604, 1300, 1360
DraI TTTAAA 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 1137
Ecl136II GAGCTC 1 cut(s) 908
Eco105I TACGTA 1 cut(s) 476
Eco24I GRGCYC 2 cut(s) 910, 1056
Eco47I GGWCC 2 cut(s) 383, 698
Eco53kI GAGCTC 1 cut(s) 908
Eco88I CYCGRG 1 cut(s) 718
EcoICRI GAGCTC 1 cut(s) 908
EcoO109I RGGNCCY 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 993
EcoT22I ATGCAT 3 cut(s) 252, 452, 939
EcoT38I GRGCYC 2 cut(s) 910, 1056
FaeI CATG 7 cut(s) 239, 254, 276, 357, 450, 454, 472
FalI AAGNNNNNCTT 2 cut(s) 1022, 1054
FaqI GGGAC 2 cut(s) 134, 1133
FatI CATG 7 cut(s) 235, 250, 272, 353, 446, 450, 468
FauI CCCGC 1 cut(s) 447
FauNDI CATATG 1 cut(s) 574
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 7 cut(s) 189, 786, 789, 821, 897, 1098, 1422
FokI GGATG 4 cut(s) 332, 924, 1127, 1272
FriOI GRGCYC 2 cut(s) 910, 1056
Fsp4HI GCNGC 7 cut(s) 189, 786, 789, 821, 897, 1098, 1422
FspAI RTGCGCAY 1 cut(s) 234
FspBI CTAG 2 cut(s) 92, 990
FspI TGCGCA 1 cut(s) 234
GlaI GCGC 2 cut(s) 97, 234
GluI GCNGC 7 cut(s) 189, 786, 789, 821, 897, 1098, 1422
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 2 cut(s) 1139, 1232
HapII CCGG 2 cut(s) 140, 261
HgaI GACGC 1 cut(s) 1273
HhaI GCGC 2 cut(s) 98, 235
Hin1II CATG 7 cut(s) 239, 254, 276, 357, 450, 454, 472
Hin6I GCGC 2 cut(s) 96, 233
HinP1I GCGC 2 cut(s) 96, 233
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 8 cut(s) 363, 391, 458, 672, 1016, 1091, 1133, 1282
HpaII CCGG 2 cut(s) 140, 261
HphI GGTGA 1 cut(s) 997
Hpy166II GTNNAC 4 cut(s) 404, 567, 958, 1005
Hpy188I TCNGA 4 cut(s) 82, 1113, 1240, 1394
Hpy188III TCNNGA 5 cut(s) 360, 884, 911, 990, 1315
Hpy8I GTNNAC 4 cut(s) 404, 567, 958, 1005
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 4 cut(s) 176, 686, 1243, 1345
HpyCH4III ACNGT 4 cut(s) 491, 597, 690, 1074
HpyCH4IV ACGT 3 cut(s) 227, 475, 1001
HpyF10VI GCNNNNNNNGC 6 cut(s) 447, 785, 820, 905, 1019, 1136
HpyF3I CTNAG 5 cut(s) 73, 561, 694, 738, 1014
HpySE526I ACGT 3 cut(s) 227, 475, 1001
Hsp92II CATG 7 cut(s) 239, 254, 276, 357, 450, 454, 472
HspAI GCGC 2 cut(s) 96, 233
Kzo9I GATC 4 cut(s) 493, 604, 1300, 1360
LmnI GCTCC 3 cut(s) 27, 254, 905
Lsp1109I GCAGC 6 cut(s) 175, 772, 775, 832, 883, 1408
LweI GCATC 6 cut(s) 45, 225, 310, 846, 946, 1385
MaeI CTAG 2 cut(s) 92, 990
MaeII ACGT 3 cut(s) 227, 475, 1001
MaeIII GTNAC 3 cut(s) 471, 725, 1261
MalI GATC 4 cut(s) 495, 606, 1302, 1362
MboI GATC 4 cut(s) 493, 604, 1300, 1360
MboII GAAGA 1 cut(s) 311
MhlI GDGCHC 3 cut(s) 695, 910, 1056
MluCI AATT 7 cut(s) 42, 753, 993, 1057, 1146, 1220, 1352
MlyI GAGTC 3 cut(s) 357, 1025, 1085
MmeI TCCRAC 1 cut(s) 1022
Mph1103I ATGCAT 3 cut(s) 252, 452, 939
MseI TTAA 4 cut(s) 114, 711, 1103, 1426
MslI CAYNNNNRTG 2 cut(s) 489, 688
MspA1I CMGCKG 2 cut(s) 785, 1130
MspI CCGG 2 cut(s) 140, 261
Mva1269I GAATGC 1 cut(s) 985
MwoI GCNNNNNNNGC 6 cut(s) 447, 785, 820, 905, 1019, 1136
NdeI CATATG 1 cut(s) 574
NdeII GATC 4 cut(s) 493, 604, 1300, 1360
NlaIII CATG 7 cut(s) 239, 254, 276, 357, 450, 454, 472
NlaIV GGNNCC 2 cut(s) 256, 1036
NmuCI GTSAC 1 cut(s) 1261
NsbI TGCGCA 1 cut(s) 234
NsiI ATGCAT 3 cut(s) 252, 452, 939
NspI RCATGY 4 cut(s) 239, 276, 450, 454
NspV TTCGAA 1 cut(s) 591
OliI CACNNNNGTG 2 cut(s) 489, 688
PaeI GCATGC 3 cut(s) 239, 450, 454
PaeR7I CTCGAG 1 cut(s) 718
PctI GAATGC 1 cut(s) 985
PfeI GAWTC 5 cut(s) 391, 458, 672, 1133, 1282
PflMI CCANNNNNTGG 1 cut(s) 354
PkrI GCNGC 7 cut(s) 190, 787, 790, 822, 898, 1099, 1423
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 3 cut(s) 357, 1024, 1085
PpsI GAGTC 3 cut(s) 357, 1024, 1085
Ppu21I YACGTR 2 cut(s) 228, 476
PpuMI RGGWCCY 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 910
Psp1406I AACGTT 1 cut(s) 1001
Psp5II RGGWCCY 1 cut(s) 383
PspN4I GGNNCC 2 cut(s) 256, 1036
PspPI GGNCC 2 cut(s) 383, 698
PspPPI RGGWCCY 1 cut(s) 383
PspXI VCTCGAGB 1 cut(s) 718
PstI CTGCAG 2 cut(s) 193, 1281
PstNI CAGNNNCTG 1 cut(s) 788
PvuI CGATCG 1 cut(s) 607
PvuII CAGCTG 2 cut(s) 785, 1130
RsaI GTAC 5 cut(s) 55, 478, 651, 959, 1368
RsaNI GTAC 5 cut(s) 54, 477, 650, 958, 1367
RseI CAYNNNNRTG 2 cut(s) 489, 688
SacI GAGCTC 1 cut(s) 910
SaqAI TTAA 4 cut(s) 114, 711, 1103, 1426
SatI GCNGC 7 cut(s) 189, 786, 789, 821, 897, 1098, 1422
Sau3AI GATC 4 cut(s) 493, 604, 1300, 1360
Sau96I GGNCC 2 cut(s) 383, 698
SchI GAGTC 3 cut(s) 357, 1025, 1085
SduI GDGCHC 3 cut(s) 695, 910, 1056
SfaNI GCATC 6 cut(s) 45, 225, 310, 846, 946, 1385
SfcI CTRYAG 2 cut(s) 189, 1277
Sfr274I CTCGAG 1 cut(s) 718
SfuI TTCGAA 1 cut(s) 591
SinI GGWCC 2 cut(s) 383, 698
SlaI CTCGAG 1 cut(s) 718
SmiMI CAYNNNNRTG 2 cut(s) 489, 688
SmlI CTYRAG 5 cut(s) 718, 774, 900, 963, 1244
SmoI CTYRAG 5 cut(s) 718, 774, 900, 963, 1244
SnaBI TACGTA 1 cut(s) 476
SphI GCATGC 3 cut(s) 239, 450, 454
Sse9I AATT 7 cut(s) 42, 753, 993, 1057, 1146, 1220, 1352
SsiI CCGC 2 cut(s) 454, 1098
SspI AATATT 1 cut(s) 410
SspMI CTAG 2 cut(s) 92, 990
SstI GAGCTC 1 cut(s) 910
TaaI ACNGT 4 cut(s) 491, 597, 690, 1074
TaiI ACGT 3 cut(s) 230, 478, 1004
TaqI TCGA 8 cut(s) 510, 591, 607, 719, 868, 912, 1181, 1316
TasI AATT 7 cut(s) 42, 753, 993, 1057, 1146, 1220, 1352
TatI WGTACW 1 cut(s) 649
TauI GCSGC 1 cut(s) 1100
TfiI GAWTC 5 cut(s) 391, 458, 672, 1133, 1282
Tru1I TTAA 4 cut(s) 114, 711, 1103, 1426
Tru9I TTAA 4 cut(s) 114, 711, 1103, 1426
TscAI CASTG 4 cut(s) 496, 574, 1029, 1077
TseFI GTSAC 1 cut(s) 1261
TseI GCWGC 6 cut(s) 188, 785, 788, 820, 896, 1421
Tsp45I GTSAC 1 cut(s) 1261
TspDTI ATGAA 4 cut(s) 644, 746, 986, 1036
TspRI CASTG 4 cut(s) 496, 574, 1029, 1077
Van91I CCANNNNNTGG 1 cut(s) 354
VpaK11BI GGWCC 2 cut(s) 383, 698
XapI RAATTY 2 cut(s) 993, 1146
XbaI TCTAGA 1 cut(s) 989
XceI RCATGY 4 cut(s) 239, 276, 450, 454
XhoI CTCGAG 1 cut(s) 718
XmiI GTMKAC 1 cut(s) 403
XspI CTAG 2 cut(s) 92, 990
Zsp2I ATGCAT 3 cut(s) 252, 452, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.