Rh7AG441700

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000569
Physical Location & Seq
Forward (+)
59858716 .. 59977170
118455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG441700.1

Sequence Viewer

Length: 1371 bp
ATGACTTCCTCCTCTGCTTATCAGGTGGAAGGCGCCGCCGACCAAGATGGGAGGACCCCAAGCATATTTGACACCTTTGCCTATGCTGGGAATTATGATGGAGCAAATGGAGATGTTGCATGTGATGCATATCACAAATACAAGGAAGATGTGCAGCTCATGGTGGATACTGGTTTGGAGGCATATAGATTTTCCATCTCATGGTCAAGGCTTATACCAAATGGAAGAGGAGCTGTCAATCCAAAGGGTCTTCAATATTACAATAATCTTATTGATGAACTAATCACCCATGGAATCCAGCCACATGTTACTTTACACCACTGTGATCTCCCACAGCAACTTGAAGATGATTATGGAGGATGGGTTAGTCGAAAGATTATAAAGGACTTCACTGCATATGCAGATGTGTGCTTTGAAAAGTTTGGTGATAGAGTTTCACATTGGACTACTATGAATGAGGCTAATGTTTTTGTACTTGGAGGTTATGACATTGGATTTCTTCCACCACAACGCTGTTCAGCTCCATTTGGTGTAAATTGCTCAAGGGGTAACTCCTCAACTGAGCCATACATGGCTGCTCATCATATGTTGCTAGCTCATGCATCGGCTGCCAGATTGTACAAGAAAATTTACCAGGAGAAACAGCAAGGATTTATAGGGATCAATGTCCTTGCCATTTGGTTTGTTCCTCACACGAAAACCACTGAAGATGAACTTGCCACTCAAAGAGCTCTTGACTTCTACATTGGTTGGATTATCAATCCCTTGGTGTTTGGAGATTATCCTGATTCGATGAAAAAGAATGCGGGCTCAAGACTTCCTGTCTTCACGAGTCTTGAATCCAGAAGTGTTAAGGGTTCATTTGACTTCCTGGGACTTAATTATTATTACACAGATTACATCACGGACCACTCCAGCAGCCTGAAGATGGAGAACAGAGACTTCAATGCAGATGTGGCAGCTATTCCGATATCTATGCGAAGTAATTCGTCAGCCTTTGAGTACTCGACAGCTAGCTGGGGCCTGAAAGAACTGTTGGAATATTTCAAGCAAAATTTTGGCAATCCGCCTATATACATCCATGAAAATGGTCAACGGACGCGTCGCACGTCATCATTAAAGGACTGGCTGAGGGTAAAATATTTGCGTAGACACATTGGGAGTCTGCTCACTGCTCTAAGGAATGGATCAAACACCAGAGGGTATTTTACATGGTCGTTCCTGGATTCGCTTGAGCTGTTGGGTGGCTATAAATCGAGCTACGGCCTATACTACATTGATTTGGACGACCCTAATCTGAAAAGACATCCTAAACTCTCTGCTCATTGGTATTCCCAGTTTTTAAAGAGGCAAAATATCACTGCAGTCTGA

Protein Analysis

456

Amino Acids

51.85

Weight (kDa)

6.73

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 2 - 451 4.2e-136 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 201
AccI GTMKAC 1 cut(s) 1150
AccII CGCG 1 cut(s) 1102
AciI CCGC 3 cut(s) 36, 806, 1067
AclWI GGATC 2 cut(s) 668, 1195
AcsI RAATTY 2 cut(s) 627, 1054
AcuI CTGAAG 2 cut(s) 726, 944
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 3 cut(s) 474, 620, 1004
AfiI CCNNNNNNNGG 3 cut(s) 87, 201, 928
AflIII ACRYGT 2 cut(s) 304, 1100
AgsI TTSAA 6 cut(s) 254, 344, 416, 839, 946, 1048
AhdI GACNNNNNGTC 1 cut(s) 821
AjiI CACGTC 1 cut(s) 1110
AjnI CCWGG 3 cut(s) 633, 870, 1221
AjuI GAANNNNNNNTTGG 2 cut(s) 1019, 1051
AleI CACNNNNGTG 1 cut(s) 321
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 1 cut(s) 933
AlwI GGATC 2 cut(s) 668, 1195
AoxI GGCC 2 cut(s) 1021, 1264
ApeKI GCWGC 5 cut(s) 154, 575, 608, 918, 959
ApoI RAATTY 2 cut(s) 627, 1054
Asp700I GAANNNNTTC 1 cut(s) 985
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 3 cut(s) 54, 907, 1021
AsuHPI GGTGA 2 cut(s) 277, 437
AsuNHI GCTAGC 2 cut(s) 592, 1013
AvaII GGWCC 2 cut(s) 54, 907
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 2 cut(s) 733, 812
BauI CACGAG 1 cut(s) 829
BbsI GAAGAC 2 cut(s) 242, 817
Bbv12I GWGCWC 1 cut(s) 733
BbvCI CCTCAGC 1 cut(s) 1130
BbvI GCAGC 5 cut(s) 166, 562, 595, 930, 971
BccI CCATC 5 cut(s) 41, 92, 203, 354, 922
BceAI ACGGC 1 cut(s) 1279
BcgI CGANNNNNNTGC 2 cut(s) 958, 992
BciT130I CCWGG 3 cut(s) 635, 872, 1223
BciVI GTATCC 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 933
BfaI CTAG 2 cut(s) 593, 1014
BfmI CTRYAG 1 cut(s) 1362
BfoI RGCGCY 1 cut(s) 36
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 6 cut(s) 36, 155, 576, 609, 919, 960
BlsI GCNGC 6 cut(s) 37, 156, 577, 610, 920, 961
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 3 cut(s) 635, 872, 1223
Bme18I GGWCC 2 cut(s) 54, 907
BmeRI GACNNNNNGTC 1 cut(s) 821
BmgBI CACGTC 1 cut(s) 1110
BmgT120I GGNCC 3 cut(s) 54, 907, 1021
BmiI GGNNCC 3 cut(s) 34, 56, 1022
BmrFI CCNGG 3 cut(s) 635, 872, 1223
BmrI ACTGGG 1 cut(s) 1330
BmsI GCATC 2 cut(s) 115, 611
BmtI GCTAGC 2 cut(s) 596, 1017
BmuI ACTGGG 1 cut(s) 1330
BpiI GAAGAC 2 cut(s) 242, 817
BpmI CTGGAG 1 cut(s) 898
Bpu10I CCTNAGC 1 cut(s) 1130
BpuEI CTTGAG 3 cut(s) 526, 796, 1253
BsaBI GATNNNNATC 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 289, 765, 871
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 201, 928
Bse1I ACTGG 3 cut(s) 175, 1130, 1336
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 3 cut(s) 635, 872, 1223
BseDI CCNNGG 3 cut(s) 289, 765, 871
BseGI GGATG 3 cut(s) 365, 1077, 1306
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 3 cut(s) 87, 201, 928
BseMII CTCAG 2 cut(s) 552, 1121
BseNI ACTGG 3 cut(s) 175, 1130, 1336
BseRI GAGGAG 2 cut(s) 243, 544
BseXI GCAGC 5 cut(s) 166, 562, 595, 930, 971
BseYI CCCAGC 2 cut(s) 86, 1017
BsgI GTGCAG 1 cut(s) 173
Bsh1236I CGCG 1 cut(s) 1102
BshFI GGCC 2 cut(s) 1023, 1266
BshNI GGYRCC 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 733
BslFI GGGAC 1 cut(s) 888
BslI CCNNNNNNNGG 3 cut(s) 87, 201, 928
BsmAI GTCTC 1 cut(s) 933
BsmFI GGGAC 1 cut(s) 888
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 2 cut(s) 1023, 1266
Bsp1286I GDGCHC 2 cut(s) 733, 812
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 3 cut(s) 325, 660, 1187
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 3 cut(s) 36, 806, 1067
BspANI GGCC 2 cut(s) 1023, 1266
BspCNI CTCAG 2 cut(s) 553, 1122
BspFNI CGCG 1 cut(s) 1102
BspLI GGNNCC 3 cut(s) 34, 56, 1022
BspMAI CTGCAG 1 cut(s) 1366
BspOI GCTAGC 2 cut(s) 596, 1017
BspPI GGATC 2 cut(s) 668, 1195
BspT107I GGYRCC 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 3 cut(s) 175, 1130, 1336
BssECI CCNNGG 3 cut(s) 289, 765, 871
BssMI GATC 3 cut(s) 325, 660, 1187
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 829
BssT1I CCWWGG 2 cut(s) 289, 765
Bst2BI CACGAG 1 cut(s) 829
Bst2UI CCWGG 3 cut(s) 635, 872, 1223
Bst4CI ACNGT 2 cut(s) 323, 1035
Bst6I CTCTTC 1 cut(s) 220
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 2 cut(s) 125, 608
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 594, 808, 1015
BstDEI CTNAG 3 cut(s) 561, 1130, 1178
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 3 cut(s) 365, 1077, 1306
BstFNI CGCG 1 cut(s) 1102
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 328, 663, 1190
BstMAI GTCTC 1 cut(s) 933
BstMBI GATC 3 cut(s) 325, 660, 1187
BstMWI GCNNNNNNNGC 3 cut(s) 125, 608, 956
BstNI CCWGG 3 cut(s) 635, 872, 1223
BstNSI RCATGY 2 cut(s) 123, 308
BstSCI CCNGG 3 cut(s) 633, 870, 1221
BstSFI CTRYAG 1 cut(s) 1362
BstUI CGCG 1 cut(s) 1102
BstV1I GCAGC 5 cut(s) 166, 562, 595, 930, 971
BstV2I GAAGAC 2 cut(s) 242, 817
BstXI CCANNNNNNTGG 1 cut(s) 1088
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 2 cut(s) 1023, 1266
BtgI CCRYGG 1 cut(s) 289
BtrI CACGTC 1 cut(s) 1110
BtsCI GGATG 3 cut(s) 365, 1077, 1306
BtsI GCAGTG 3 cut(s) 390, 1170, 1359
BtsIMutI CAGTG 5 cut(s) 319, 390, 702, 1170, 1359
Cac8I GCNNGC 3 cut(s) 594, 808, 1015
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 3 cut(s) 54, 907, 1021
CseI GACGC 2 cut(s) 1091, 1108
Csp6I GTAC 3 cut(s) 473, 619, 1003
CviAII CATG 9 cut(s) 120, 160, 201, 290, 305, 571, 599, 1082, 1212
CviQI GTAC 3 cut(s) 473, 619, 1003
DdeI CTNAG 3 cut(s) 561, 1130, 1178
DinI GGCGCC 1 cut(s) 34
DpnI GATC 3 cut(s) 327, 662, 1189
DpnII GATC 3 cut(s) 325, 660, 1187
DraI TTTAAA 1 cut(s) 1344
DriI GACNNNNNGTC 1 cut(s) 821
Eam1104I CTCTTC 1 cut(s) 220
Eam1105I GACNNNNNGTC 1 cut(s) 821
EarI CTCTTC 1 cut(s) 220
EciI GGCGGA 1 cut(s) 1056
Ecl136II GAGCTC 1 cut(s) 731
Eco130I CCWWGG 2 cut(s) 289, 765
Eco24I GRGCYC 2 cut(s) 733, 812
Eco32I GATATC 1 cut(s) 972
Eco47I GGWCC 2 cut(s) 54, 907
Eco53kI GAGCTC 1 cut(s) 731
Eco57I CTGAAG 2 cut(s) 726, 944
EcoICRI GAGCTC 1 cut(s) 731
EcoO109I RGGNCCY 2 cut(s) 54, 1021
EcoRII CCWGG 3 cut(s) 633, 870, 1221
EcoRV GATATC 1 cut(s) 972
EcoT14I CCWWGG 2 cut(s) 289, 765
EcoT22I ATGCAT 2 cut(s) 130, 604
EcoT38I GRGCYC 2 cut(s) 733, 812
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 289, 765
FaeI CATG 9 cut(s) 123, 163, 204, 293, 308, 574, 602, 1085, 1215
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 1 cut(s) 888
FatI CATG 9 cut(s) 119, 159, 200, 289, 304, 570, 598, 1081, 1211
FauI CCCGC 1 cut(s) 799
FauNDI CATATG 2 cut(s) 397, 585
FblI GTMKAC 1 cut(s) 1150
Fnu4HI GCNGC 6 cut(s) 36, 155, 576, 609, 919, 960
FokI GGATG 3 cut(s) 372, 1064, 1293
FriOI GRGCYC 2 cut(s) 733, 812
Fsp4HI GCNGC 6 cut(s) 36, 155, 576, 609, 919, 960
FspBI CTAG 2 cut(s) 593, 1014
GlaI GCGC 1 cut(s) 34
GluI GCNGC 6 cut(s) 36, 155, 576, 609, 919, 960
GsaI CCCAGC 2 cut(s) 90, 1021
GsuI CTGGAG 1 cut(s) 898
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 2 cut(s) 1023, 1266
HgaI GACGC 2 cut(s) 1091, 1108
HhaI GCGC 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 9 cut(s) 123, 163, 204, 293, 308, 574, 602, 1085, 1215
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HincII GTYRAC 1 cut(s) 1094
HindII GTYRAC 1 cut(s) 1094
HinfI GANTC 6 cut(s) 294, 788, 832, 839, 1162, 1226
HphI GGTGA 2 cut(s) 277, 437
Hpy166II GTNNAC 2 cut(s) 1094, 1151
Hpy188I TCNGA 3 cut(s) 969, 1299, 1370
Hpy188III TCNNGA 6 cut(s) 734, 785, 813, 829, 836, 843
Hpy8I GTNNAC 2 cut(s) 1094, 1151
Hpy99I CGWCG 1 cut(s) 1107
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 2 cut(s) 323, 1035
HpyCH4IV ACGT 1 cut(s) 1109
HpyCH4V TGCA 8 cut(s) 119, 128, 154, 395, 401, 602, 950, 1364
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 608, 956
HpyF3I CTNAG 3 cut(s) 561, 1130, 1178
HpySE526I ACGT 1 cut(s) 1109
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 9 cut(s) 123, 163, 204, 293, 308, 574, 602, 1085, 1215
HspAI GCGC 1 cut(s) 33
KasI GGCGCC 1 cut(s) 32
Kzo9I GATC 3 cut(s) 325, 660, 1187
LmnI GCTCC 3 cut(s) 101, 230, 526
Lsp1109I GCAGC 5 cut(s) 166, 562, 595, 930, 971
LweI GCATC 2 cut(s) 115, 611
MaeI CTAG 2 cut(s) 593, 1014
MaeII ACGT 1 cut(s) 1109
MaeIII GTNAC 2 cut(s) 307, 548
MalI GATC 3 cut(s) 327, 662, 1189
MboI GATC 3 cut(s) 325, 660, 1187
MboII GAAGA 8 cut(s) 158, 237, 242, 356, 491, 719, 817, 937
MhlI GDGCHC 2 cut(s) 733, 812
MluCI AATT 6 cut(s) 91, 535, 627, 880, 985, 1054
MluI ACGCGT 1 cut(s) 1100
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 2 cut(s) 841, 1171
MmeI TCCRAC 2 cut(s) 731, 1017
Mph1103I ATGCAT 2 cut(s) 130, 604
MroXI GAANNNNTTC 1 cut(s) 985
MseI TTAA 4 cut(s) 852, 879, 1118, 1343
MslI CAYNNNNRTG 2 cut(s) 321, 1086
MspR9I CCNGG 3 cut(s) 635, 872, 1223
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 3 cut(s) 635, 872, 1223
MvnI CGCG 1 cut(s) 1102
MwoI GCNNNNNNNGC 3 cut(s) 125, 608, 956
NarI GGCGCC 1 cut(s) 33
NcoI CCATGG 1 cut(s) 289
NdeI CATATG 2 cut(s) 397, 585
NdeII GATC 3 cut(s) 325, 660, 1187
NheI GCTAGC 2 cut(s) 592, 1013
NlaIII CATG 9 cut(s) 123, 163, 204, 293, 308, 574, 602, 1085, 1215
NlaIV GGNNCC 3 cut(s) 34, 56, 1022
NsiI ATGCAT 2 cut(s) 130, 604
NspI RCATGY 2 cut(s) 123, 308
OliI CACNNNNGTG 1 cut(s) 321
PciI ACATGT 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 1106
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 985
PfeI GAWTC 4 cut(s) 294, 788, 839, 1226
PflMI CCANNNNNTGG 1 cut(s) 201
PfoI TCCNGGA 1 cut(s) 1221
PkrI GCNGC 6 cut(s) 37, 156, 577, 610, 920, 961
PleI GAGTC 2 cut(s) 840, 1170
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 2 cut(s) 840, 1170
PpuMI RGGWCCY 1 cut(s) 54
PscI ACATGT 1 cut(s) 304
PsiI TTATAA 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 733
Psp5II RGGWCCY 1 cut(s) 54
Psp6I CCWGG 3 cut(s) 633, 870, 1221
PspFI CCCAGC 2 cut(s) 86, 1017
PspGI CCWGG 3 cut(s) 633, 870, 1221
PspN4I GGNNCC 3 cut(s) 34, 56, 1022
PspPI GGNCC 3 cut(s) 54, 907, 1021
PspPPI RGGWCCY 1 cut(s) 54
PstI CTGCAG 1 cut(s) 1366
RsaI GTAC 3 cut(s) 474, 620, 1004
RsaNI GTAC 3 cut(s) 473, 619, 1003
RseI CAYNNNNRTG 2 cut(s) 321, 1086
SacI GAGCTC 1 cut(s) 733
SaqAI TTAA 4 cut(s) 852, 879, 1118, 1343
SatI GCNGC 6 cut(s) 36, 155, 576, 609, 919, 960
Sau3AI GATC 3 cut(s) 325, 660, 1187
Sau96I GGNCC 3 cut(s) 54, 907, 1021
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 2 cut(s) 841, 1171
ScrFI CCNGG 3 cut(s) 635, 872, 1223
SduI GDGCHC 2 cut(s) 733, 812
SfaNI GCATC 2 cut(s) 115, 611
SfcI CTRYAG 1 cut(s) 1362
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 2 cut(s) 54, 907
SmiMI CAYNNNNRTG 2 cut(s) 321, 1086
SmlI CTYRAG 3 cut(s) 541, 811, 1232
SmoI CTYRAG 3 cut(s) 541, 811, 1232
Sse9I AATT 6 cut(s) 91, 535, 627, 880, 985, 1054
SsiI CCGC 3 cut(s) 36, 806, 1067
SspDI GGCGCC 1 cut(s) 32
SspI AATATT 3 cut(s) 257, 1043, 1142
SspMI CTAG 2 cut(s) 593, 1014
SstI GAGCTC 1 cut(s) 733
StyD4I CCNGG 3 cut(s) 633, 870, 1221
StyI CCWWGG 2 cut(s) 289, 765
TaaI ACNGT 2 cut(s) 323, 1035
TaiI ACGT 1 cut(s) 1112
TaqI TCGA 4 cut(s) 370, 791, 1007, 1256
TasI AATT 6 cut(s) 91, 535, 627, 880, 985, 1054
TatI WGTACW 3 cut(s) 472, 618, 1002
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 4 cut(s) 294, 788, 839, 1226
Tru1I TTAA 4 cut(s) 852, 879, 1118, 1343
Tru9I TTAA 4 cut(s) 852, 879, 1118, 1343
TscAI CASTG 5 cut(s) 326, 397, 709, 1177, 1366
TseI GCWGC 5 cut(s) 154, 575, 608, 918, 959
TspDTI ATGAA 6 cut(s) 291, 467, 726, 809, 849, 1098
TspGWI ACGGA 2 cut(s) 920, 1111
TspRI CASTG 5 cut(s) 326, 397, 709, 1177, 1366
Van91I CCANNNNNTGG 1 cut(s) 201
VpaK11BI GGWCC 2 cut(s) 54, 907
XapI RAATTY 2 cut(s) 627, 1054
XceI RCATGY 2 cut(s) 123, 308
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 1 cut(s) 985
XspI CTAG 2 cut(s) 593, 1014
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 2 cut(s) 130, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.