Belongs to the glycosyl hydrolase 1 family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Glyco_hydro_1 | PF00232 | 47 - 504 | 1.3e-140 | Glycosyl hydrolase family 1 |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 237, 1479 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 357 |
| AccI | GTMKAC | 1 cut(s) | 406 |
| AccII | CGCG | 1 cut(s) | 588 |
| AciI | CCGC | 1 cut(s) | 1074 |
| AclI | AACGTT | 1 cut(s) | 1151 |
| AclWI | GGATC | 3 cut(s) | 1143, 1351, 1439 |
| AcuI | CTGAAG | 1 cut(s) | 1520 |
| AfaI | GTAC | 4 cut(s) | 64, 630, 938, 1487 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 357 |
| AgsI | TTSAA | 1 cut(s) | 1002 |
| AjnI | CCWGG | 1 cut(s) | 1367 |
| AleI | CACNNNNGTG | 1 cut(s) | 477 |
| AluBI | AGCT | 6 cut(s) | 86, 677, 764, 887, 1393, 1416 |
| AluI | AGCT | 6 cut(s) | 86, 677, 764, 887, 1393, 1416 |
| Alw26I | GTCTC | 2 cut(s) | 1089, 1377 |
| AlwI | GGATC | 3 cut(s) | 1143, 1351, 1439 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 767, 1538 |
| Ama87I | CYCGRG | 1 cut(s) | 632 |
| AoxI | GGCC | 1 cut(s) | 1420 |
| ApeKI | GCWGC | 4 cut(s) | 764, 767, 799, 875 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 137, 169, 884, 916 |
| AsiSI | GCGATCGC | 1 cut(s) | 586 |
| AspLEI | GCGC | 3 cut(s) | 107, 238, 1480 |
| AspS9I | GGNCC | 3 cut(s) | 196, 386, 1284 |
| AsuHPI | GGTGA | 1 cut(s) | 976 |
| AsuII | TTCGAA | 1 cut(s) | 570 |
| AvaI | CYCGRG | 1 cut(s) | 632 |
| AvaII | GGWCC | 3 cut(s) | 196, 386, 1284 |
| BanII | GRGCYC | 1 cut(s) | 723 |
| BauI | CACGAG | 1 cut(s) | 794 |
| BbsI | GAAGAC | 1 cut(s) | 990 |
| BbvI | GCAGC | 4 cut(s) | 751, 754, 811, 862 |
| BccI | CCATC | 4 cut(s) | 359, 510, 867, 1394 |
| BceAI | ACGGC | 2 cut(s) | 1231, 1435 |
| BciT130I | CCWGG | 1 cut(s) | 1369 |
| BciVI | GTATCC | 2 cut(s) | 316, 1170 |
| BclI | TGATCA | 1 cut(s) | 1540 |
| BcoDI | GTCTC | 2 cut(s) | 1089, 1377 |
| BfaI | CTAG | 2 cut(s) | 101, 969 |
| BfoI | RGCGCY | 1 cut(s) | 108 |
| BfuI | GTATCC | 2 cut(s) | 316, 1170 |
| BisI | GCNGC | 5 cut(s) | 765, 768, 800, 876, 1074 |
| BlsI | GCNGC | 5 cut(s) | 766, 769, 801, 877, 1075 |
| Bme1390I | CCNGG | 1 cut(s) | 1369 |
| Bme18I | GGWCC | 3 cut(s) | 196, 386, 1284 |
| BmeT110I | CYCGRG | 1 cut(s) | 632 |
| BmgT120I | GGNCC | 3 cut(s) | 196, 386, 1284 |
| BmiI | GGNNCC | 1 cut(s) | 259 |
| BmrFI | CCNGG | 1 cut(s) | 1369 |
| BmsI | GCATC | 4 cut(s) | 228, 767, 825, 1525 |
| BpiI | GAAGAC | 1 cut(s) | 990 |
| Bpu14I | TTCGAA | 1 cut(s) | 570 |
| BpuEI | CTTGAG | 2 cut(s) | 927, 1409 |
| Bsa29I | ATCGAT | 1 cut(s) | 1434 |
| BsaBI | GATNNNNATC | 2 cut(s) | 285, 1539 |
| BsaI | GGTCTC | 1 cut(s) | 1377 |
| BsaJI | CCNNGG | 3 cut(s) | 699, 1059, 1172 |
| BsaWI | WCCGGW | 2 cut(s) | 142, 263 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 504, 534, 1268, 1298 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 357 |
| Bse1I | ACTGG | 3 cut(s) | 27, 331, 987 |
| Bse8I | GATNNNNATC | 2 cut(s) | 285, 1539 |
| BseBI | CCWGG | 1 cut(s) | 1369 |
| BseCI | ATCGAT | 1 cut(s) | 1434 |
| BseDI | CCNNGG | 3 cut(s) | 699, 1059, 1172 |
| BseGI | GGATG | 2 cut(s) | 521, 1405 |
| BseJI | GATNNNNATC | 2 cut(s) | 285, 1539 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 357 |
| BseMII | CTCAG | 2 cut(s) | 708, 984 |
| BseNI | ACTGG | 3 cut(s) | 27, 331, 987 |
| BseRI | GAGGAG | 3 cut(s) | 23, 157, 700 |
| BseXI | GCAGC | 4 cut(s) | 751, 754, 811, 862 |
| BseYI | CCCAGC | 1 cut(s) | 242 |
| BsgI | GTGCAG | 1 cut(s) | 753 |
| Bsh1236I | CGCG | 1 cut(s) | 588 |
| Bsh1285I | CGRYCG | 1 cut(s) | 586 |
| BshFI | GGCC | 1 cut(s) | 1422 |
| BshVI | ATCGAT | 1 cut(s) | 1434 |
| BsiEI | CGRYCG | 1 cut(s) | 586 |
| BsiHKCI | CYCGRG | 1 cut(s) | 632 |
| BsiSI | CCGG | 2 cut(s) | 143, 264 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 357 |
| BsmAI | GTCTC | 2 cut(s) | 1089, 1377 |
| BsmI | GAATGC | 1 cut(s) | 964 |
| BsnI | GGCC | 1 cut(s) | 1422 |
| Bso31I | GGTCTC | 1 cut(s) | 1377 |
| BsoBI | CYCGRG | 1 cut(s) | 632 |
| Bsp119I | TTCGAA | 1 cut(s) | 570 |
| Bsp1286I | GDGCHC | 1 cut(s) | 723 |
| Bsp143I | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| BspACI | CCGC | 1 cut(s) | 1074 |
| BspANI | GGCC | 1 cut(s) | 1422 |
| BspCNI | CTCAG | 2 cut(s) | 709, 985 |
| BspDI | ATCGAT | 1 cut(s) | 1434 |
| BspFNI | CGCG | 1 cut(s) | 588 |
| BspLI | GGNNCC | 1 cut(s) | 259 |
| BspPI | GGATC | 3 cut(s) | 1143, 1351, 1439 |
| BspT104I | TTCGAA | 1 cut(s) | 570 |
| BspTNI | GGTCTC | 1 cut(s) | 1377 |
| BsrI | ACTGG | 3 cut(s) | 27, 331, 987 |
| BssECI | CCNNGG | 3 cut(s) | 699, 1059, 1172 |
| BssMI | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| BssSI | CACGAG | 1 cut(s) | 794 |
| BssT1I | CCWWGG | 3 cut(s) | 699, 1059, 1172 |
| Bst2BI | CACGAG | 1 cut(s) | 794 |
| Bst2UI | CCWGG | 1 cut(s) | 1369 |
| Bst4CI | ACNGT | 5 cut(s) | 479, 576, 663, 1191, 1319 |
| Bst6I | CTCTTC | 1 cut(s) | 376 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 764 |
| BstBI | TTCGAA | 1 cut(s) | 570 |
| BstC8I | GCNNGC | 2 cut(s) | 240, 964 |
| BstDEI | CTNAG | 2 cut(s) | 717, 993 |
| BstF5I | GGATG | 2 cut(s) | 521, 1405 |
| BstFNI | CGCG | 1 cut(s) | 588 |
| BstH2I | RGCGCY | 1 cut(s) | 108 |
| BstHHI | GCGC | 3 cut(s) | 107, 238, 1480 |
| BstKTI | GATC | 6 cut(s) | 484, 586, 1138, 1346, 1447, 1543 |
| BstMAI | GTCTC | 2 cut(s) | 1089, 1377 |
| BstMBI | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| BstMCI | CGRYCG | 1 cut(s) | 586 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 764, 799, 884, 1112 |
| BstNI | CCWGG | 1 cut(s) | 1369 |
| BstNSI | RCATGY | 1 cut(s) | 242 |
| BstSCI | CCNGG | 1 cut(s) | 1367 |
| BstUI | CGCG | 1 cut(s) | 588 |
| BstV1I | GCAGC | 4 cut(s) | 751, 754, 811, 862 |
| BstV2I | GAAGAC | 1 cut(s) | 990 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1244 |
| Bsu15I | ATCGAT | 1 cut(s) | 1434 |
| BsuI | GTATCC | 2 cut(s) | 316, 1170 |
| BsuRI | GGCC | 1 cut(s) | 1422 |
| BsuTUI | ATCGAT | 1 cut(s) | 1434 |
| BtsCI | GGATG | 2 cut(s) | 521, 1405 |
| BtsI | GCAGTG | 1 cut(s) | 546 |
| BtsIMutI | CAGTG | 3 cut(s) | 484, 546, 994 |
| Cac8I | GCNNGC | 2 cut(s) | 240, 964 |
| CaiI | CAGNNNCTG | 2 cut(s) | 767, 1538 |
| CfoI | GCGC | 3 cut(s) | 107, 238, 1480 |
| Cfr13I | GGNCC | 3 cut(s) | 196, 386, 1284 |
| ClaI | ATCGAT | 1 cut(s) | 1434 |
| CseI | GACGC | 1 cut(s) | 1269 |
| CsiI | ACCWGGT | 1 cut(s) | 1367 |
| Csp6I | GTAC | 4 cut(s) | 63, 629, 937, 1486 |
| CviAII | CATG | 8 cut(s) | 239, 254, 357, 461, 727, 755, 1238, 1304 |
| CviQI | GTAC | 4 cut(s) | 63, 629, 937, 1486 |
| DdeI | CTNAG | 2 cut(s) | 717, 993 |
| DpnI | GATC | 6 cut(s) | 483, 585, 1137, 1345, 1446, 1542 |
| DpnII | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| DraI | TTTAAA | 2 cut(s) | 118, 1455 |
| Eam1104I | CTCTTC | 1 cut(s) | 376 |
| EarI | CTCTTC | 1 cut(s) | 376 |
| Eco130I | CCWWGG | 3 cut(s) | 699, 1059, 1172 |
| Eco24I | GRGCYC | 1 cut(s) | 723 |
| Eco31I | GGTCTC | 1 cut(s) | 1377 |
| Eco47I | GGWCC | 3 cut(s) | 196, 386, 1284 |
| Eco57I | CTGAAG | 1 cut(s) | 1520 |
| Eco88I | CYCGRG | 1 cut(s) | 632 |
| EcoO109I | RGGNCCY | 1 cut(s) | 386 |
| EcoRII | CCWGG | 1 cut(s) | 1367 |
| EcoT14I | CCWWGG | 3 cut(s) | 699, 1059, 1172 |
| EcoT22I | ATGCAT | 2 cut(s) | 255, 760 |
| EcoT38I | GRGCYC | 1 cut(s) | 723 |
| ErhI | CCWWGG | 3 cut(s) | 699, 1059, 1172 |
| FaeI | CATG | 8 cut(s) | 242, 257, 360, 464, 730, 758, 1241, 1307 |
| FatI | CATG | 8 cut(s) | 238, 253, 356, 460, 726, 754, 1237, 1303 |
| FauNDI | CATATG | 1 cut(s) | 553 |
| FbaI | TGATCA | 1 cut(s) | 1540 |
| FblI | GTMKAC | 1 cut(s) | 406 |
| Fnu4HI | GCNGC | 5 cut(s) | 765, 768, 800, 876, 1074 |
| FokI | GGATG | 2 cut(s) | 528, 1412 |
| FriOI | GRGCYC | 1 cut(s) | 723 |
| Fsp4HI | GCNGC | 5 cut(s) | 765, 768, 800, 876, 1074 |
| FspAI | RTGCGCAY | 1 cut(s) | 237 |
| FspBI | CTAG | 2 cut(s) | 101, 969 |
| FspI | TGCGCA | 2 cut(s) | 237, 1479 |
| GlaI | GCGC | 3 cut(s) | 106, 237, 1479 |
| GluI | GCNGC | 5 cut(s) | 765, 768, 800, 876, 1074 |
| GsaI | CCCAGC | 1 cut(s) | 246 |
| HaeII | RGCGCY | 1 cut(s) | 108 |
| HaeIII | GGCC | 1 cut(s) | 1422 |
| HapII | CCGG | 2 cut(s) | 143, 264 |
| HgaI | GACGC | 1 cut(s) | 1269 |
| HhaI | GCGC | 3 cut(s) | 107, 238, 1480 |
| Hin1II | CATG | 8 cut(s) | 242, 257, 360, 464, 730, 758, 1241, 1307 |
| Hin6I | GCGC | 3 cut(s) | 105, 236, 1478 |
| HinP1I | GCGC | 3 cut(s) | 105, 236, 1478 |
| HincII | GTYRAC | 1 cut(s) | 1250 |
| HindII | GTYRAC | 1 cut(s) | 1250 |
| HpaII | CCGG | 2 cut(s) | 143, 264 |
| HphI | GGTGA | 1 cut(s) | 976 |
| Hpy166II | GTNNAC | 4 cut(s) | 196, 407, 984, 1250 |
| Hpy188I | TCNGA | 6 cut(s) | 932, 978, 1031, 1164, 1500, 1534 |
| Hpy188III | TCNNGA | 7 cut(s) | 363, 666, 863, 969, 1120, 1287, 1448 |
| Hpy8I | GTNNAC | 4 cut(s) | 196, 407, 984, 1250 |
| Hpy99I | CGWCG | 3 cut(s) | 503, 982, 1263 |
| HpyAV | CCTTC | 3 cut(s) | 144, 179, 665 |
| HpyCH4III | ACNGT | 5 cut(s) | 479, 576, 663, 1191, 1319 |
| HpyCH4IV | ACGT | 3 cut(s) | 980, 1151, 1522 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 764, 799, 884, 1112 |
| HpyF3I | CTNAG | 2 cut(s) | 717, 993 |
| HpySE526I | ACGT | 3 cut(s) | 980, 1151, 1522 |
| Hsp92II | CATG | 8 cut(s) | 242, 257, 360, 464, 730, 758, 1241, 1307 |
| HspAI | GCGC | 3 cut(s) | 105, 236, 1478 |
| Ksp22I | TGATCA | 1 cut(s) | 1540 |
| Kzo9I | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| LmnI | GCTCC | 4 cut(s) | 36, 257, 682, 701 |
| Lsp1109I | GCAGC | 4 cut(s) | 751, 754, 811, 862 |
| LweI | GCATC | 4 cut(s) | 228, 767, 825, 1525 |
| MabI | ACCWGGT | 1 cut(s) | 1367 |
| MaeI | CTAG | 2 cut(s) | 101, 969 |
| MaeII | ACGT | 3 cut(s) | 980, 1151, 1522 |
| MaeIII | GTNAC | 3 cut(s) | 463, 663, 704 |
| MalI | GATC | 6 cut(s) | 483, 585, 1137, 1345, 1446, 1542 |
| MboI | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| MboII | GAAGA | 5 cut(s) | 314, 393, 990, 1196, 1513 |
| MhlI | GDGCHC | 1 cut(s) | 723 |
| MluCI | AATT | 7 cut(s) | 51, 426, 691, 732, 739, 1036, 1264 |
| MlyI | GAGTC | 2 cut(s) | 1004, 1026 |
| MmeI | TCCRAC | 5 cut(s) | 887, 999, 1001, 1173, 1254 |
| Mph1103I | ATGCAT | 2 cut(s) | 255, 760 |
| MseI | TTAA | 5 cut(s) | 117, 690, 1326, 1334, 1454 |
| MslI | CAYNNNNRTG | 2 cut(s) | 477, 1242 |
| MspA1I | CMGCKG | 1 cut(s) | 764 |
| MspI | CCGG | 2 cut(s) | 143, 264 |
| MspR9I | CCNGG | 1 cut(s) | 1369 |
| Mva1269I | GAATGC | 1 cut(s) | 964 |
| MvaI | CCWGG | 1 cut(s) | 1369 |
| MvnI | CGCG | 1 cut(s) | 588 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 764, 799, 884, 1112 |
| NdeI | CATATG | 1 cut(s) | 553 |
| NdeII | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| NlaIII | CATG | 8 cut(s) | 242, 257, 360, 464, 730, 758, 1241, 1307 |
| NlaIV | GGNNCC | 1 cut(s) | 259 |
| NmuCI | GTSAC | 1 cut(s) | 663 |
| NsbI | TGCGCA | 2 cut(s) | 237, 1479 |
| NsiI | ATGCAT | 2 cut(s) | 255, 760 |
| NspI | RCATGY | 1 cut(s) | 242 |
| NspV | TTCGAA | 1 cut(s) | 570 |
| OliI | CACNNNNGTG | 1 cut(s) | 477 |
| PaeI | GCATGC | 1 cut(s) | 242 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1440 |
| PctI | GAATGC | 1 cut(s) | 964 |
| PfeI | GAWTC | 8 cut(s) | 394, 450, 651, 916, 1109, 1290, 1382, 1409 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 357 |
| PkrI | GCNGC | 5 cut(s) | 766, 769, 801, 877, 1075 |
| Ple19I | CGATCG | 1 cut(s) | 586 |
| PleI | GAGTC | 2 cut(s) | 1003, 1026 |
| PpsI | GAGTC | 2 cut(s) | 1003, 1026 |
| PpuMI | RGGWCCY | 1 cut(s) | 386 |
| Psp1406I | AACGTT | 1 cut(s) | 1151 |
| Psp5II | RGGWCCY | 1 cut(s) | 386 |
| Psp6I | CCWGG | 1 cut(s) | 1367 |
| PspFI | CCCAGC | 1 cut(s) | 242 |
| PspGI | CCWGG | 1 cut(s) | 1367 |
| PspN4I | GGNNCC | 1 cut(s) | 259 |
| PspPI | GGNCC | 3 cut(s) | 196, 386, 1284 |
| PspPPI | RGGWCCY | 1 cut(s) | 386 |
| PstNI | CAGNNNCTG | 2 cut(s) | 767, 1538 |
| PvuI | CGATCG | 1 cut(s) | 586 |
| PvuII | CAGCTG | 1 cut(s) | 764 |
| RgaI | GCGATCGC | 1 cut(s) | 586 |
| RsaI | GTAC | 4 cut(s) | 64, 630, 938, 1487 |
| RsaNI | GTAC | 4 cut(s) | 63, 629, 937, 1486 |
| RseI | CAYNNNNRTG | 2 cut(s) | 477, 1242 |
| SaqAI | TTAA | 5 cut(s) | 117, 690, 1326, 1334, 1454 |
| SatI | GCNGC | 5 cut(s) | 765, 768, 800, 876, 1074 |
| Sau3AI | GATC | 6 cut(s) | 481, 583, 1135, 1343, 1444, 1540 |
| Sau96I | GGNCC | 3 cut(s) | 196, 386, 1284 |
| SchI | GAGTC | 2 cut(s) | 1004, 1026 |
| ScrFI | CCNGG | 1 cut(s) | 1369 |
| SduI | GDGCHC | 1 cut(s) | 723 |
| SexAI | ACCWGGT | 1 cut(s) | 1367 |
| SfaAI | GCGATCGC | 1 cut(s) | 586 |
| SfaNI | GCATC | 4 cut(s) | 228, 767, 825, 1525 |
| SfuI | TTCGAA | 1 cut(s) | 570 |
| SgfI | GCGATCGC | 1 cut(s) | 586 |
| SinI | GGWCC | 3 cut(s) | 196, 386, 1284 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 477, 1242 |
| SmlI | CTYRAG | 2 cut(s) | 942, 1388 |
| SmoI | CTYRAG | 2 cut(s) | 942, 1388 |
| SphI | GCATGC | 1 cut(s) | 242 |
| Sse9I | AATT | 7 cut(s) | 51, 426, 691, 732, 739, 1036, 1264 |
| SsiI | CCGC | 1 cut(s) | 1074 |
| SspI | AATATT | 2 cut(s) | 413, 1298 |
| SspMI | CTAG | 2 cut(s) | 101, 969 |
| StyD4I | CCNGG | 1 cut(s) | 1367 |
| StyI | CCWWGG | 3 cut(s) | 699, 1059, 1172 |
| TaaI | ACNGT | 5 cut(s) | 479, 576, 663, 1191, 1319 |
| TaiI | ACGT | 3 cut(s) | 983, 1154, 1525 |
| TaqI | TCGA | 6 cut(s) | 498, 570, 847, 1258, 1293, 1434 |
| TasI | AATT | 7 cut(s) | 51, 426, 691, 732, 739, 1036, 1264 |
| TatI | WGTACW | 1 cut(s) | 628 |
| TauI | GCSGC | 1 cut(s) | 1076 |
| TfiI | GAWTC | 8 cut(s) | 394, 450, 651, 916, 1109, 1290, 1382, 1409 |
| Tru1I | TTAA | 5 cut(s) | 117, 690, 1326, 1334, 1454 |
| Tru9I | TTAA | 5 cut(s) | 117, 690, 1326, 1334, 1454 |
| TscAI | CASTG | 3 cut(s) | 484, 553, 994 |
| TseFI | GTSAC | 1 cut(s) | 663 |
| TseI | GCWGC | 4 cut(s) | 764, 767, 799, 875 |
| Tsp45I | GTSAC | 1 cut(s) | 663 |
| TspRI | CASTG | 3 cut(s) | 484, 553, 994 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 357 |
| VpaK11BI | GGWCC | 3 cut(s) | 196, 386, 1284 |
| XbaI | TCTAGA | 1 cut(s) | 968 |
| XceI | RCATGY | 1 cut(s) | 242 |
| XmiI | GTMKAC | 1 cut(s) | 406 |
| XspI | CTAG | 2 cut(s) | 101, 969 |
| Zsp2I | ATGCAT | 2 cut(s) | 255, 760 |