Rmu_sc0000556.1_g000042

Protein SGT1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000556.1
OG0001767
Physical Location & Seq
Reverse (-)
205992 .. 209364
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1701 bp
atgagtctggttgacgaaagaaccatattgaggagcatcgtaacaaaacaggatggtctgttccgccgcaataaggtgattggcgctcgtttaatctcgtatgagataaagaacttcttcgtgccagaatcatactacactggaactatccgatttgacaacgagacacaaaaagaagcctgttttttcaaatttgcacagaatgaaggagaagtagctaagcatatttttaaagcagcttccaaattgaatcaagatggaatcctacagccctggtttctcaccaataacaaattagagaagacgtggattttatgttacgagcacttcaactctcttctcagtgagctaccagataacaaaatttttgagctgcctgaaaattggcgtctctctgttaggaactctatgaagaccatcaagtacatacatgaacgctatcttcttcatctgggtttaaaaaaaagaagtagctacgccctaattaatgacaagaagaatggtttcaaaatcaaaatcctctttgatcgtaattcatcgactgagaattcggtgtacgacataaaaaaggcaaagttggatgattggaaagattataaggacgtgctggagtttctgttgaagtcattgccacaaacggatgacctaaagttgtttctaaattattttggtctggaccaaaaagggaaatatgatgtattcgttgatcgactcattgcacatccttttctcttgtgtgctgaagaaaacatgaattttattgcaaacgtacagcatgccattattacccaaaggatcagttttttaagtggtgaaaaattcaaagagtatgagaattgggatgagaaacttgatgattggctgaaaggtatatactcacagcaaatagtagatggattcagttataacgtcatgagtgtaacaggtctcatttggtttttgagagatgtttatgtccacaattgtgataaatttgaagagattgattgtcacttgatgcaatcgtttcctgggaagttcctctggaatgaatttgagatgtgcacaaggccagaggttatccctgcaaatgcagagttaagtgtcagcattgatgttcctggagaagagacatatcattttcagcctcgcctgtttggaaaggtattgcatctatttgtggtgctgaggaatcttcttaatttgttaagatatcatgtatatctagagaattctttggagtaccctttccaatttgatgttttgtctaccaaagttgacattcgcctggccaaagctgaacctatacattcaagagatgtccagttcagcaaggataaccctgttccactaaaggttaatgccccagttattggcgtccaaaggccatcttacccatcctccaaaccaaaatgggtagattgggacaagcttgaagcccaagaaaaagatgagaagcttgatggtgatgcagctttgaacaaatttttccaagacatattcaaggatgctgatgaggacacaagaagggccatgagaaaatcttttgtggagttgaacggaacggtgctttcgacaatctggaaaaaagtgggcaccaagaaggtcgaggaaagtgctcctgatggtatggagatgaggaaatgggagtaccaagagctcaagttggacagtgcttgtaacctatttggagtgcctttcgttttggtggacgctgtttag

Protein Analysis

566

Amino Acids

66.35

Weight (kDa)

6.24

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SGS PF05002 467 - 542 5.4e-29 SGS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 597, 906
AccB1I GGYRCC 1 cut(s) 1574
AccB7I CCANNNNNTGG 1 cut(s) 1352
AccI GTMKAC 1 cut(s) 1247
AciI CCGC 2 cut(s) 64, 67
AclWI GGATC 1 cut(s) 803
AcoI YGGCCR 1 cut(s) 1269
AcsI RAATTY 9 cut(s) 191, 363, 547, 754, 818, 971, 1031, 1210, 1463
AcuI CTGAAG 1 cut(s) 762
AcyI GRCGYC 2 cut(s) 388, 1356
AfaI GTAC 5 cut(s) 425, 557, 771, 1223, 1631
AfiI CCNNNNNNNGG 3 cut(s) 30, 73, 1352
AjiI CACGTC 2 cut(s) 306, 604
AjnI CCWGG 4 cut(s) 272, 1009, 1099, 1266
AleI CACNNNNGTG 1 cut(s) 963
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
Alw21I GWGCWC 4 cut(s) 327, 1046, 1600, 1641
Alw26I GTCTC 4 cut(s) 158, 395, 932, 1103
Alw44I GTGCAC 1 cut(s) 1042
AlwI GGATC 1 cut(s) 803
AoxI GGCC 4 cut(s) 1049, 1269, 1364, 1509
ApaLI GTGCAC 1 cut(s) 1042
ApeKI GCWGC 3 cut(s) 236, 373, 1451
ApoI RAATTY 9 cut(s) 191, 363, 547, 754, 818, 971, 1031, 1210, 1463
ArsI GACNNNNNNTTYG 2 cut(s) 532, 564
AseI ATTAAT 1 cut(s) 486
Asp700I GAANNNNTTC 2 cut(s) 116, 503
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 676, 1509
AsuHPI GGTGA 4 cut(s) 88, 274, 824, 1457
AvaII GGWCC 1 cut(s) 676
BaeGI GKGCMC 2 cut(s) 1046, 1577
BalI TGGCCA 1 cut(s) 1271
BanI GGYRCC 1 cut(s) 1574
BanII GRGCYC 1 cut(s) 1641
BbsI GAAGAC 2 cut(s) 308, 419
Bbv12I GWGCWC 4 cut(s) 327, 1046, 1600, 1641
BbvCI CCTCAGC 1 cut(s) 1166
BbvI GCAGC 3 cut(s) 248, 360, 1463
BccI CCATC 8 cut(s) 47, 251, 425, 887, 1375, 1384, 1436, 1598
BcgI CGANNNNNNTGC 2 cut(s) 1577, 1611
BciT130I CCWGG 4 cut(s) 274, 1011, 1101, 1268
BcoDI GTCTC 4 cut(s) 158, 395, 932, 1103
BfaI CTAG 1 cut(s) 1205
BfmI CTRYAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 4 cut(s) 67, 237, 374, 1452
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 4 cut(s) 68, 238, 375, 1453
Bme1390I CCNGG 4 cut(s) 274, 1011, 1101, 1268
Bme18I GGWCC 1 cut(s) 676
BmgBI CACGTC 2 cut(s) 306, 604
BmgT120I GGNCC 2 cut(s) 676, 1509
BmiI GGNNCC 1 cut(s) 1576
BmrFI CCNGG 4 cut(s) 274, 1011, 1101, 1268
BmrI ACTGGG 1 cut(s) 1340
BmsI GCATC 5 cut(s) 45, 987, 1159, 1438, 1477
BmuI ACTGGG 1 cut(s) 1340
BpiI GAAGAC 2 cut(s) 308, 419
BpmI CTGGAG 2 cut(s) 629, 1122
Bpu10I CCTNAGC 1 cut(s) 1166
Bpu1102I GCTNAGC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 1625
BsaHI GRCGYC 2 cut(s) 388, 1356
BsaI GGTCTC 1 cut(s) 932
BsaJI CCNNGG 2 cut(s) 272, 1010
BsaXI ACNNNNNCTCC 4 cut(s) 602, 632, 1364, 1394
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 73, 1352
Bse1I ACTGG 3 cut(s) 145, 1303, 1346
Bse3DI GCAATG 2 cut(s) 626, 714
BseBI CCWGG 4 cut(s) 274, 1011, 1101, 1268
BseDI CCNNGG 2 cut(s) 272, 1010
BseGI GGATG 7 cut(s) 58, 586, 646, 721, 847, 1376, 1492
BseLI CCNNNNNNNGG 3 cut(s) 30, 73, 1352
BseMI GCAATG 2 cut(s) 626, 714
BseMII CTCAG 3 cut(s) 355, 534, 1157
BseNI ACTGG 3 cut(s) 145, 1303, 1346
BseRI GAGGAG 1 cut(s) 46
BseSI GKGCMC 2 cut(s) 1046, 1577
BseXI GCAGC 3 cut(s) 248, 360, 1463
BshFI GGCC 4 cut(s) 1051, 1271, 1366, 1511
BshNI GGYRCC 1 cut(s) 1574
BsiHKAI GWGCWC 4 cut(s) 327, 1046, 1600, 1641
BslFI GGGAC 1 cut(s) 1418
BslI CCNNNNNNNGG 3 cut(s) 30, 73, 1352
BsmAI GTCTC 4 cut(s) 158, 395, 932, 1103
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 1418
BsnI GGCC 4 cut(s) 1051, 1271, 1366, 1511
Bso31I GGTCTC 1 cut(s) 932
Bsp1286I GDGCHC 5 cut(s) 327, 1046, 1577, 1600, 1641
Bsp143I GATC 3 cut(s) 526, 706, 795
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 2 cut(s) 64, 67
BspANI GGCC 4 cut(s) 1051, 1271, 1366, 1511
BspCNI CTCAG 3 cut(s) 354, 535, 1158
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 1 cut(s) 1576
BspPI GGATC 1 cut(s) 803
BspT107I GGYRCC 1 cut(s) 1574
BspTNI GGTCTC 1 cut(s) 932
BsrDI GCAATG 2 cut(s) 626, 714
BsrI ACTGG 3 cut(s) 145, 1303, 1346
BssECI CCNNGG 2 cut(s) 272, 1010
BssMI GATC 3 cut(s) 526, 706, 795
BssNI GRCGYC 2 cut(s) 388, 1356
Bst2UI CCWGG 4 cut(s) 274, 1011, 1101, 1268
Bst4CI ACNGT 2 cut(s) 1546, 1652
Bst6I CTCTTC 3 cut(s) 342, 972, 1101
BstACI GRCGYC 2 cut(s) 388, 1356
BstC8I GCNNGC 1 cut(s) 777
BstDEI CTNAG 4 cut(s) 219, 341, 543, 1166
BstF5I GGATG 7 cut(s) 58, 586, 646, 721, 847, 1376, 1492
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 3 cut(s) 529, 709, 798
BstMAI GTCTC 4 cut(s) 158, 395, 932, 1103
BstMBI GATC 3 cut(s) 526, 706, 795
BstNI CCWGG 4 cut(s) 274, 1011, 1101, 1268
BstNSI RCATGY 1 cut(s) 779
BstSCI CCNGG 4 cut(s) 272, 1009, 1099, 1266
BstSFI CTRYAG 1 cut(s) 266
BstSLI GKGCMC 2 cut(s) 1046, 1577
BstV1I GCAGC 3 cut(s) 248, 360, 1463
BstV2I GAAGAC 2 cut(s) 308, 419
BsuRI GGCC 4 cut(s) 1051, 1271, 1366, 1511
BtrI CACGTC 2 cut(s) 306, 604
BtsCI GGATG 7 cut(s) 58, 586, 646, 721, 847, 1376, 1492
BtsIMutI CAGTG 3 cut(s) 138, 349, 1657
Cac8I GCNNGC 1 cut(s) 777
CciI TCATGA 1 cut(s) 912
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 676, 1509
CseI GACGC 2 cut(s) 377, 1345
Csp6I GTAC 5 cut(s) 424, 556, 770, 1222, 1630
CviAII CATG 6 cut(s) 431, 751, 776, 913, 1196, 1513
CviQI GTAC 5 cut(s) 424, 556, 770, 1222, 1630
DdeI CTNAG 4 cut(s) 219, 341, 543, 1166
DpnI GATC 3 cut(s) 528, 708, 797
DpnII GATC 3 cut(s) 526, 706, 795
DraI TTTAAA 2 cut(s) 232, 459
EaeI YGGCCR 1 cut(s) 1269
Eam1104I CTCTTC 3 cut(s) 342, 972, 1101
EarI CTCTTC 3 cut(s) 342, 972, 1101
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 1639
Eco24I GRGCYC 1 cut(s) 1641
Eco31I GGTCTC 1 cut(s) 932
Eco32I GATATC 1 cut(s) 1193
Eco47I GGWCC 1 cut(s) 676
Eco53kI GAGCTC 1 cut(s) 1639
Eco57I CTGAAG 1 cut(s) 762
EcoICRI GAGCTC 1 cut(s) 1639
EcoRI GAATTC 2 cut(s) 547, 1210
EcoRII CCWGG 4 cut(s) 272, 1009, 1099, 1266
EcoRV GATATC 1 cut(s) 1193
EcoT38I GRGCYC 1 cut(s) 1641
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 6 cut(s) 434, 754, 779, 916, 1199, 1516
FalI AAGNNNNNCTT 4 cut(s) 101, 133, 1354, 1386
FaqI GGGAC 1 cut(s) 1418
FatI CATG 6 cut(s) 430, 750, 775, 912, 1195, 1512
FblI GTMKAC 1 cut(s) 1247
Fnu4HI GCNGC 4 cut(s) 67, 237, 374, 1452
FokI GGATG 7 cut(s) 65, 593, 653, 708, 854, 1363, 1499
FriOI GRGCYC 1 cut(s) 1641
Fsp4HI GCNGC 4 cut(s) 67, 237, 374, 1452
FspBI CTAG 1 cut(s) 1205
GlaI GCGC 1 cut(s) 85
GluI GCNGC 4 cut(s) 67, 237, 374, 1452
GsuI CTGGAG 2 cut(s) 629, 1122
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 4 cut(s) 1051, 1271, 1366, 1511
HgaI GACGC 2 cut(s) 377, 1345
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 2 cut(s) 388, 1356
Hin1II CATG 6 cut(s) 434, 754, 779, 916, 1199, 1516
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 2 cut(s) 13, 1258
HindII GTYRAC 2 cut(s) 13, 1258
HindIII AAGCTT 2 cut(s) 1409, 1436
HinfI GANTC 7 cut(s) 4, 128, 250, 261, 711, 897, 1171
HphI GGTGA 4 cut(s) 88, 274, 824, 1457
Hpy166II GTNNAC 7 cut(s) 13, 556, 958, 1044, 1248, 1258, 1690
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 8 cut(s) 254, 674, 913, 1024, 1205, 1293, 1561, 1601
Hpy8I GTNNAC 7 cut(s) 13, 556, 958, 1044, 1248, 1258, 1690
HpyAV CCTTC 3 cut(s) 200, 1500, 1576
HpyCH4III ACNGT 2 cut(s) 1546, 1652
HpyCH4IV ACGT 4 cut(s) 305, 603, 768, 909
HpyCH4V TGCA 9 cut(s) 197, 719, 764, 1000, 1044, 1067, 1073, 1150, 1451
HpyF3I CTNAG 4 cut(s) 219, 341, 543, 1166
HpySE526I ACGT 4 cut(s) 305, 603, 768, 909
Hsp92I GRCGYC 2 cut(s) 388, 1356
Hsp92II CATG 6 cut(s) 434, 754, 779, 916, 1199, 1516
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 3 cut(s) 526, 706, 795
LmnI GCTCC 2 cut(s) 33, 1603
Lsp1109I GCAGC 3 cut(s) 248, 360, 1463
LweI GCATC 5 cut(s) 45, 987, 1159, 1438, 1477
MaeI CTAG 1 cut(s) 1205
MaeII ACGT 4 cut(s) 305, 603, 768, 909
MaeIII GTNAC 5 cut(s) 40, 317, 919, 989, 1658
MalI GATC 3 cut(s) 528, 708, 797
MboI GATC 3 cut(s) 526, 706, 795
MfeI CAATTG 1 cut(s) 961
MhlI GDGCHC 5 cut(s) 327, 1046, 1577, 1600, 1641
MlsI TGGCCA 1 cut(s) 1271
MluNI TGGCCA 1 cut(s) 1271
MlyI GAGTC 2 cut(s) 13, 705
MmeI TCCRAC 2 cut(s) 558, 1626
Mox20I TGGCCA 1 cut(s) 1271
MroXI GAANNNNTTC 2 cut(s) 116, 503
MscI TGGCCA 1 cut(s) 1271
MseI TTAA 9 cut(s) 92, 231, 458, 486, 806, 1079, 1179, 1187, 1338
MslI CAYNNNNRTG 1 cut(s) 963
Msp20I TGGCCA 1 cut(s) 1271
MspR9I CCNGG 4 cut(s) 274, 1011, 1101, 1268
MunI CAATTG 1 cut(s) 961
MvaI CCWGG 4 cut(s) 274, 1011, 1101, 1268
NdeII GATC 3 cut(s) 526, 706, 795
NlaIII CATG 6 cut(s) 434, 754, 779, 916, 1199, 1516
NlaIV GGNNCC 1 cut(s) 1576
NmuCI GTSAC 1 cut(s) 989
NspI RCATGY 1 cut(s) 779
OliI CACNNNNGTG 1 cut(s) 963
PaeI GCATGC 1 cut(s) 779
PagI TCATGA 1 cut(s) 912
PcsI WCGNNNNNNNCGW 1 cut(s) 1550
PdmI GAANNNNTTC 2 cut(s) 116, 503
PfeI GAWTC 5 cut(s) 128, 250, 261, 897, 1171
PflMI CCANNNNNTGG 1 cut(s) 1352
PfoI TCCNGGA 1 cut(s) 1099
PkrI GCNGC 4 cut(s) 68, 238, 375, 1453
PleI GAGTC 2 cut(s) 12, 705
PpsI GAGTC 2 cut(s) 12, 705
PshBI ATTAAT 1 cut(s) 486
PsiI TTATAA 2 cut(s) 597, 906
Psp124BI GAGCTC 1 cut(s) 1641
Psp6I CCWGG 4 cut(s) 272, 1009, 1099, 1266
PspGI CCWGG 4 cut(s) 272, 1009, 1099, 1266
PspN4I GGNNCC 1 cut(s) 1576
PspPI GGNCC 2 cut(s) 676, 1509
RsaI GTAC 5 cut(s) 425, 557, 771, 1223, 1631
RsaNI GTAC 5 cut(s) 424, 556, 770, 1222, 1630
RseI CAYNNNNRTG 1 cut(s) 963
SacI GAGCTC 1 cut(s) 1641
SaqAI TTAA 9 cut(s) 92, 231, 458, 486, 806, 1079, 1179, 1187, 1338
SatI GCNGC 4 cut(s) 67, 237, 374, 1452
Sau3AI GATC 3 cut(s) 526, 706, 795
Sau96I GGNCC 2 cut(s) 676, 1509
SchI GAGTC 2 cut(s) 13, 705
ScrFI CCNGG 4 cut(s) 274, 1011, 1101, 1268
SduI GDGCHC 5 cut(s) 327, 1046, 1577, 1600, 1641
SfaNI GCATC 5 cut(s) 45, 987, 1159, 1438, 1477
SfcI CTRYAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 963
SmlI CTYRAG 1 cut(s) 1640
SmoI CTYRAG 1 cut(s) 1640
SphI GCATGC 1 cut(s) 779
SsiI CCGC 2 cut(s) 64, 67
SspMI CTAG 1 cut(s) 1205
SstI GAGCTC 1 cut(s) 1641
StyD4I CCNGG 4 cut(s) 272, 1009, 1099, 1266
TaaI ACNGT 2 cut(s) 1546, 1652
TaiI ACGT 4 cut(s) 308, 606, 771, 912
TaqI TCGA 4 cut(s) 539, 709, 1553, 1587
TatI WGTACW 1 cut(s) 423
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 5 cut(s) 128, 250, 261, 897, 1171
Tru1I TTAA 9 cut(s) 92, 231, 458, 486, 806, 1079, 1179, 1187, 1338
Tru9I TTAA 9 cut(s) 92, 231, 458, 486, 806, 1079, 1179, 1187, 1338
TscAI CASTG 3 cut(s) 145, 349, 1657
TseFI GTSAC 1 cut(s) 989
TseI GCWGC 3 cut(s) 236, 373, 1451
Tsp45I GTSAC 1 cut(s) 989
TspDTI ATGAA 7 cut(s) 219, 425, 437, 447, 525, 767, 1044
TspGWI ACGGA 2 cut(s) 653, 1554
TspRI CASTG 3 cut(s) 145, 349, 1657
Van91I CCANNNNNTGG 1 cut(s) 1352
VneI GTGCAC 1 cut(s) 1042
VpaK11BI GGWCC 1 cut(s) 676
VspI ATTAAT 1 cut(s) 486
XapI RAATTY 9 cut(s) 191, 363, 547, 754, 818, 971, 1031, 1210, 1463
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 1247
XmnI GAANNNNTTC 2 cut(s) 116, 503
XspI CTAG 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.