Rorug07G0092500

receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001767
Physical Location & Seq
Reverse (-)
7246933 .. 7251578
4646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0092500.1

Sequence Viewer

Length: 1638 bp
ATGAGTGCTCTTCTAAAAAACCCCAGAAGAGAAAGCGTGGAGATTGACGACGACGACATGATGCCGCCGGCGGAAGAGAACGGCGACCCAGATAGCAGACAAGACCAAGAAGACGCCCTCATCGCTCTGATTGAGCACCGCACTCAGGAAGTGGAACACAATCGCCGGAGACGGGAGTATTACCAATCCAAGCTTGTTGAAGCAGAGAAGAGGTTGAATGAATCTCAAACTAAATTGGCTCGACTTCGATCCCAAAACAAGGCAGTGTCGACTAAGGCGTCTCCGAGTAATGGAATTAAAAGTGTAAAGGAGGAGCGCAGATCAATGAGTCCTATCCCTTTAAGAAGCCAGCCTCAATCTAAAACAGAACTTTTAATTCCTGCTGTCACTCCAAAAGTTTCCCAACCTCCGAAAGTGGCAGGGTCTAGTGGGAAGGCACAAGTTAGTTTTAGTTCTCAAGCCAGCCCCGCAGTTTCTAGTCAGTCTATTAGTGCTAGCAAAGAAAAAGGAGATAAGTCTTACAGAAGTTCGTCAAAGCAGGATGTTGAAGTTCAAGATAAAGGGACAAAACGGAAGTTCGAGGAGAAAGAACATAAAGAATTAATTCCGATGGTTCGTACTAGCTCCAAACCCTCTATAATCCAGTGCCATTCAAACAATCACATATCCAGTCATCACAAGAGAAAGTTGAGATGTCTTTATTTGTGTCCAGTGAATGATCAGCTTTTTGCAACCAGCGCTTTGGATGGACTGGTCAACTTGTGGGAAGTTCGAGGCAGGGGGTCAGGTGCTTCTCTACTTAGTACTACAGATTGCCAATCCCCAAAGCAAAGGAGATGGCCAGAAGATATTGCCTGGCATCCCCATGGAAACAGCCTATTTTCTGTGTATACTGCTGATGGTGGGGAATCACAAGTATCAGTTCTAAATCTAAATCGAACACAAGGGAAACCTCGTGTTACTTTCTTGGAAGCCAAGCCTCATGTGAAAGGGATTATTAACAGCATAGTTTTCCCACCTTGGGAGAATACCTGCTTTGTCACTGGTGGTAGTGATCATGCTGTTGTACTCTGGAAAGAGACGGATGCAGAAAATGTCTGGAAACCAAAGCAATTGCATAGGAGTATACATTCATCTGCTGTTATGGGCGTTGCTGGGATGTGGCAAAAGCAAATTATACTATCTGTGGGAGCAGACAAGAGGATTGTTGGCTTCGATGCTAATATGGAAAGAACAGAGTTTAAGCATCAGATAGATAGTAAATGCATGAGTGTTTTGCCAAATCCTTGCGACTTTAATCTATTCATGGTTCAAGCAGGTACTCATGAGAAGCAGCTCCGACTTTATGATATCAGACTGAGGCAAACAGAGATACATGCTTTTGGGTGGAAGCAAGAGAGCAGTGATTCTCAATCGGCTCTGATAAATCAAGCTTGGTCTCCTGATGGTTTATACATCTCATCCGGTACTGCTGATCCTATGATTCACATATTTGATATCAGGTATAACGCCCAGAAGCCTTCCCAATCCATTAAGGCTCATCAGAAACGGGTTTTCAAAGCTGTATGGCTCCGCACTAGCCCCCTTCTCATTTCTATATCCTCTGATTTGATGGTTGGTTTACACAAAATCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

545

Amino Acids

61.22

Weight (kDa)

9.35

Isoelectric Point (pI)

63.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 221 - 408 2.8e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 224 - 452 3.5e-06 WDR5 beta-propeller domain
WD40_Prp19 PF24814 325 - 502 5e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 382 - 538 8.1e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 388 - 541 3.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 437 - 541 5.9e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 478 - 542 7.2e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 478 - 542 4e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 277
Acc36I ACCTGC 2 cut(s) 1040, 1307
AccI GTMKAC 3 cut(s) 269, 890, 1126
AciI CCGC 5 cut(s) 65, 71, 139, 468, 1573
AclWI GGATC 2 cut(s) 243, 1469
AcoI YGGCCR 1 cut(s) 839
AcyI GRCGYC 2 cut(s) 114, 278
AfaI GTAC 5 cut(s) 619, 805, 1068, 1321, 1468
AfeI AGCGCT 1 cut(s) 739
AfiI CCNNNNNNNGG 5 cut(s) 145, 172, 259, 290, 1021
AgsI TTSAA 7 cut(s) 200, 217, 548, 554, 654, 1313, 1558
AjnI CCWGG 1 cut(s) 854
AluBI AGCT 6 cut(s) 193, 624, 724, 1336, 1433, 1562
AluI AGCT 6 cut(s) 193, 624, 724, 1336, 1433, 1562
Alw21I GWGCWC 2 cut(s) 10, 138
Alw26I GTCTC 4 cut(s) 163, 285, 1073, 1443
AlwI GGATC 2 cut(s) 243, 1469
Aor51HI AGCGCT 1 cut(s) 739
AoxI GGCC 1 cut(s) 839
ApeKI GCWGC 1 cut(s) 1333
AseI ATTAAT 1 cut(s) 602
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 2 cut(s) 318, 740
AsuNHI GCTAGC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 841
BauI CACGAG 1 cut(s) 954
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 10, 138
BbvI GCAGC 1 cut(s) 1345
BccI CCATC 6 cut(s) 604, 740, 831, 893, 1439, 1606
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 856
BclI TGATCA 2 cut(s) 718, 1054
BcoDI GTCTC 4 cut(s) 163, 285, 1073, 1443
BfaI CTAG 5 cut(s) 426, 477, 495, 621, 1578
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 1 cut(s) 741
BfuAI ACCTGC 2 cut(s) 1040, 1307
BisI GCNGC 2 cut(s) 65, 1334
BlsI GCNGC 2 cut(s) 66, 1335
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 1 cut(s) 856
BmiI GGNNCC 1 cut(s) 1571
BmrFI CCNGG 1 cut(s) 856
BmsI GCATC 5 cut(s) 51, 868, 1075, 1207, 1255
BmtI GCTAGC 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 441
BsaHI GRCGYC 2 cut(s) 114, 278
BsaI GGTCTC 1 cut(s) 1443
BsaJI CCNNGG 2 cut(s) 865, 1019
BsaWI WCCGGW 1 cut(s) 1463
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 172, 259, 290, 1021
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 5 cut(s) 643, 669, 710, 756, 1048
BseBI CCWGG 1 cut(s) 856
BseDI CCNNGG 2 cut(s) 865, 1019
BseGI GGATG 6 cut(s) 547, 751, 859, 1090, 1164, 1460
BseLI CCNNNNNNNGG 5 cut(s) 145, 172, 259, 290, 1021
BseMII CTCAG 2 cut(s) 158, 1349
BseNI ACTGG 5 cut(s) 643, 669, 710, 756, 1048
BseRI GAGGAG 2 cut(s) 326, 596
BseXI GCAGC 1 cut(s) 1345
BseYI CCCAGC 1 cut(s) 1154
BshFI GGCC 1 cut(s) 841
BsiHKAI GWGCWC 2 cut(s) 10, 138
BsiSI CCGG 3 cut(s) 68, 166, 1464
BslFI GGGAC 1 cut(s) 577
BslI CCNNNNNNNGG 5 cut(s) 145, 172, 259, 290, 1021
BsmAI GTCTC 4 cut(s) 163, 285, 1073, 1443
BsmBI CGTCTC 3 cut(s) 163, 285, 1073
BsmFI GGGAC 1 cut(s) 577
BsnI GGCC 1 cut(s) 841
Bso31I GGTCTC 1 cut(s) 1443
Bsp1286I GDGCHC 2 cut(s) 10, 138
Bsp143I GATC 5 cut(s) 248, 320, 718, 1054, 1474
Bsp19I CCATGG 1 cut(s) 865
BspACI CCGC 5 cut(s) 65, 71, 139, 468, 1573
BspANI GGCC 1 cut(s) 841
BspCNI CTCAG 2 cut(s) 157, 1350
BspHI TCATGA 1 cut(s) 1324
BspLI GGNNCC 1 cut(s) 1571
BspMI ACCTGC 2 cut(s) 1040, 1307
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 243, 1469
BspQI GCTCTTC 1 cut(s) 15
BspTNI GGTCTC 1 cut(s) 1443
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 5 cut(s) 643, 669, 710, 756, 1048
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 865, 1019
BssMI GATC 5 cut(s) 248, 320, 718, 1054, 1474
BssNAI GTATAC 2 cut(s) 891, 1127
BssNI GRCGYC 2 cut(s) 114, 278
BssSI CACGAG 1 cut(s) 954
BssT1I CCWWGG 2 cut(s) 865, 1019
Bst1107I GTATAC 2 cut(s) 891, 1127
Bst2BI CACGAG 1 cut(s) 954
Bst2UI CCWGG 1 cut(s) 856
Bst6I CTCTTC 4 cut(s) 15, 22, 69, 203
BstACI GRCGYC 2 cut(s) 114, 278
BstC8I GCNNGC 4 cut(s) 69, 350, 463, 496
BstDEI CTNAG 4 cut(s) 144, 273, 800, 1358
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 6 cut(s) 547, 751, 859, 1090, 1164, 1460
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 2 cut(s) 318, 740
BstKTI GATC 5 cut(s) 251, 323, 721, 1057, 1477
BstMAI GTCTC 4 cut(s) 163, 285, 1073, 1443
BstMBI GATC 5 cut(s) 248, 320, 718, 1054, 1474
BstMWI GCNNNNNNNGC 3 cut(s) 122, 467, 737
BstNI CCWGG 1 cut(s) 856
BstNSI RCATGY 1 cut(s) 1379
BstSCI CCNGG 1 cut(s) 854
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 1 cut(s) 1345
BstV2I GAAGAC 1 cut(s) 117
BstXI CCANNNNNNTGG 1 cut(s) 742
BstZ17I GTATAC 2 cut(s) 891, 1127
BsuRI GGCC 1 cut(s) 841
BtgI CCRYGG 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 106
BtsCI GGATG 6 cut(s) 547, 751, 859, 1090, 1164, 1460
BtsI GCAGTG 2 cut(s) 270, 1408
BtsIMutI CAGTG 5 cut(s) 270, 650, 717, 1041, 1408
BveI ACCTGC 2 cut(s) 1040, 1307
Cac8I GCNNGC 4 cut(s) 69, 350, 463, 496
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 2 cut(s) 318, 740
Cfr10I RCCGGY 1 cut(s) 67
CseI GACGC 2 cut(s) 122, 267
Csp6I GTAC 5 cut(s) 618, 804, 1067, 1320, 1467
CviAII CATG 8 cut(s) 58, 866, 983, 1058, 1267, 1306, 1325, 1376
CviQI GTAC 5 cut(s) 618, 804, 1067, 1320, 1467
DdeI CTNAG 4 cut(s) 144, 273, 800, 1358
DpnI GATC 5 cut(s) 250, 322, 720, 1056, 1476
DpnII GATC 5 cut(s) 248, 320, 718, 1054, 1474
DrdI GACNNNNNNGTC 1 cut(s) 277
DseDI GACNNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 839
Eam1104I CTCTTC 4 cut(s) 15, 22, 69, 203
EarI CTCTTC 4 cut(s) 15, 22, 69, 203
EciI GGCGGA 1 cut(s) 86
Eco130I CCWWGG 2 cut(s) 865, 1019
Eco31I GGTCTC 1 cut(s) 1443
Eco32I GATATC 2 cut(s) 1351, 1498
Eco47III AGCGCT 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 854
EcoRV GATATC 2 cut(s) 1351, 1498
EcoT14I CCWWGG 2 cut(s) 865, 1019
EcoT22I ATGCAT 1 cut(s) 1268
ErhI CCWWGG 2 cut(s) 865, 1019
Esp3I CGTCTC 3 cut(s) 163, 285, 1073
FaeI CATG 8 cut(s) 61, 869, 986, 1061, 1270, 1309, 1328, 1379
FaqI GGGAC 1 cut(s) 577
FatI CATG 8 cut(s) 57, 865, 982, 1057, 1266, 1305, 1324, 1375
FauI CCCGC 1 cut(s) 475
FbaI TGATCA 2 cut(s) 718, 1054
FblI GTMKAC 3 cut(s) 269, 890, 1126
Fnu4HI GCNGC 2 cut(s) 65, 1334
FokI GGATG 6 cut(s) 554, 758, 846, 1097, 1171, 1447
Fsp4HI GCNGC 2 cut(s) 65, 1334
FspBI CTAG 5 cut(s) 426, 477, 495, 621, 1578
GlaI GCGC 2 cut(s) 317, 739
GluI GCNGC 2 cut(s) 65, 1334
GsaI CCCAGC 1 cut(s) 1158
HaeII RGCGCY 1 cut(s) 741
HaeIII GGCC 1 cut(s) 841
HapII CCGG 3 cut(s) 68, 166, 1464
HgaI GACGC 2 cut(s) 122, 267
HhaI GCGC 2 cut(s) 318, 740
Hin1I GRCGYC 2 cut(s) 114, 278
Hin1II CATG 8 cut(s) 61, 869, 986, 1061, 1270, 1309, 1328, 1379
Hin6I GCGC 2 cut(s) 316, 738
HinP1I GCGC 2 cut(s) 316, 738
HincII GTYRAC 2 cut(s) 270, 757
HindII GTYRAC 2 cut(s) 270, 757
HindIII AAGCTT 2 cut(s) 191, 1431
HinfI GANTC 5 cut(s) 221, 328, 908, 1406, 1483
HpaII CCGG 3 cut(s) 68, 166, 1464
Hpy166II GTNNAC 5 cut(s) 270, 757, 891, 1127, 1622
Hpy188III TCNNGA 6 cut(s) 146, 554, 1072, 1099, 1325, 1442
Hpy8I GTNNAC 5 cut(s) 270, 757, 891, 1127, 1622
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 3 cut(s) 427, 1530, 1595
HpyCH4V TGCA 4 cut(s) 731, 1088, 1117, 1266
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 467, 737
HpyF3I CTNAG 4 cut(s) 144, 273, 800, 1358
Hsp92I GRCGYC 2 cut(s) 114, 278
Hsp92II CATG 8 cut(s) 61, 869, 986, 1061, 1270, 1309, 1328, 1379
HspAI GCGC 2 cut(s) 316, 738
KroI GCCGGC 1 cut(s) 67
KroNI GCCGGC 1 cut(s) 69
Ksp22I TGATCA 2 cut(s) 718, 1054
Kzo9I GATC 5 cut(s) 248, 320, 718, 1054, 1474
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 5 cut(s) 313, 629, 1190, 1341, 1575
Lsp1109I GCAGC 1 cut(s) 1345
LweI GCATC 5 cut(s) 51, 868, 1075, 1207, 1255
MaeI CTAG 5 cut(s) 426, 477, 495, 621, 1578
MaeIII GTNAC 3 cut(s) 385, 958, 1039
MalI GATC 5 cut(s) 250, 322, 720, 1056, 1476
MboI GATC 5 cut(s) 248, 320, 718, 1054, 1474
MboII GAAGA 5 cut(s) 39, 86, 122, 220, 857
MfeI CAATTG 1 cut(s) 1112
MhlI GDGCHC 2 cut(s) 10, 138
MlsI TGGCCA 1 cut(s) 841
MluCI AATT 7 cut(s) 233, 294, 375, 599, 603, 1112, 1173
MluNI TGGCCA 1 cut(s) 841
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 1 cut(s) 1363
Mox20I TGGCCA 1 cut(s) 841
Mph1103I ATGCAT 1 cut(s) 1268
MreI CGCCGGCG 1 cut(s) 67
MroNI GCCGGC 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 603
MscI TGGCCA 1 cut(s) 841
MseI TTAA 8 cut(s) 297, 341, 374, 602, 999, 1242, 1296, 1533
MslI CAYNNNNRTG 1 cut(s) 864
Msp20I TGGCCA 1 cut(s) 841
MspI CCGG 3 cut(s) 68, 166, 1464
MspR9I CCNGG 1 cut(s) 856
MunI CAATTG 1 cut(s) 1112
MvaI CCWGG 1 cut(s) 856
MwoI GCNNNNNNNGC 3 cut(s) 122, 467, 737
NaeI GCCGGC 1 cut(s) 69
NcoI CCATGG 1 cut(s) 865
NdeII GATC 5 cut(s) 248, 320, 718, 1054, 1474
NgoMIV GCCGGC 1 cut(s) 67
NheI GCTAGC 1 cut(s) 494
NlaIII CATG 8 cut(s) 61, 869, 986, 1061, 1270, 1309, 1328, 1379
NlaIV GGNNCC 1 cut(s) 1571
NmuCI GTSAC 2 cut(s) 385, 1039
NsiI ATGCAT 1 cut(s) 1268
NspI RCATGY 1 cut(s) 1379
PagI TCATGA 1 cut(s) 1324
PciSI GCTCTTC 1 cut(s) 15
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PdiI GCCGGC 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 4 cut(s) 221, 908, 1406, 1483
PkrI GCNGC 2 cut(s) 66, 1335
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
PshBI ATTAAT 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 854
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 1 cut(s) 854
PspN4I GGNNCC 1 cut(s) 1571
RsaI GTAC 5 cut(s) 619, 805, 1068, 1321, 1468
RsaNI GTAC 5 cut(s) 618, 804, 1067, 1320, 1467
RseI CAYNNNNRTG 1 cut(s) 864
SalI GTCGAC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 8 cut(s) 297, 341, 374, 602, 999, 1242, 1296, 1533
SatI GCNGC 2 cut(s) 65, 1334
Sau3AI GATC 5 cut(s) 248, 320, 718, 1054, 1474
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 856
SduI GDGCHC 2 cut(s) 10, 138
SfaNI GCATC 5 cut(s) 51, 868, 1075, 1207, 1255
SfcI CTRYAG 1 cut(s) 807
SgrAI CRCCGGYG 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
Sse9I AATT 7 cut(s) 233, 294, 375, 599, 603, 1112, 1173
SsiI CCGC 5 cut(s) 65, 71, 139, 468, 1573
SspMI CTAG 5 cut(s) 426, 477, 495, 621, 1578
StyD4I CCNGG 1 cut(s) 854
StyI CCWWGG 2 cut(s) 865, 1019
TaqI TCGA 7 cut(s) 241, 247, 269, 579, 772, 937, 1215
TasI AATT 7 cut(s) 233, 294, 375, 599, 603, 1112, 1173
TatI WGTACW 2 cut(s) 803, 1066
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 4 cut(s) 221, 908, 1406, 1483
Tru1I TTAA 8 cut(s) 297, 341, 374, 602, 999, 1242, 1296, 1533
Tru9I TTAA 8 cut(s) 297, 341, 374, 602, 999, 1242, 1296, 1533
TscAI CASTG 5 cut(s) 270, 650, 717, 1048, 1408
TseFI GTSAC 2 cut(s) 385, 1039
TseI GCWGC 1 cut(s) 1333
Tsp45I GTSAC 2 cut(s) 385, 1039
TspDTI ATGAA 3 cut(s) 234, 1122, 1294
TspGWI ACGGA 2 cut(s) 586, 1097
TspRI CASTG 5 cut(s) 270, 650, 717, 1048, 1408
VspI ATTAAT 1 cut(s) 602
XceI RCATGY 1 cut(s) 1379
XmiI GTMKAC 3 cut(s) 269, 890, 1126
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 5 cut(s) 426, 477, 495, 621, 1578
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 1 cut(s) 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.