Rorug06G0012400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002407
Physical Location & Seq
Reverse (-)
1634624 .. 1636262
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0012400.1

Sequence Viewer

Length: 591 bp
ATGCTTGCGCCGCTTCTTGAGAATGGTGATGAGGCGGGTCGGGTTGGAGCGAACCGACCCGAGAGGGTTCCGACGGAGCCGCAGAGGATTTTTCATAAGCTGAACGCAGTCGCGGTGATGGTTTTGCTGCTCATCATCTTCTTTCTCCAGTACTTTCTCATCAACGGCTACATAACGCTGTTGTTGGTGTCTGTTCTTCTTGCCGGAGCGCTGGAAGTTCCGGGAAACGTACTCAGGTTGGTCGAGTCCGACTCATGGGAGAGGCGCTTCAAGGTCAAGTTCTGCTTCCTTTTCTGTGGATCTCTGAGTATGTGCCTGATCGTCTGCTTCGGGATATACTTCAACCGTTCTTGCATACCTCGATCCGTGCAGAGTCATACAGAAAATCAGATCAAAGTTAGGATCGCTGAAAAGGATAGTCAGATGTTGCCAGCACAGAGTCACAGAGCCTGTATATATGCTTGTAAACTGTTGACATGCATGTGTCCCTGTGATCCAGGTACTTCAATGAAAGTACCATTGTTATTTCACTTAATCATTTGTTTAGGCAACTGGTATCTGGAAATTAGAGTATTGTGTGTCACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.13

Weight (kDa)

8.62

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 113
AciI CCGC 4 cut(s) 11, 35, 80, 113
AclWI GGATC 4 cut(s) 307, 357, 410, 488
AfaI GTAC 4 cut(s) 152, 231, 502, 516
AfeI AGCGCT 1 cut(s) 210
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 3 cut(s) 271, 343, 507
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 1 cut(s) 100
AluI AGCT 1 cut(s) 100
AlwI GGATC 4 cut(s) 307, 357, 410, 488
AlwNI CAGNNNCTG 1 cut(s) 450
Ama87I CYCGRG 1 cut(s) 59
Aor51HI AGCGCT 1 cut(s) 210
ApeKI GCWGC 1 cut(s) 127
AspLEI GCGC 3 cut(s) 10, 211, 267
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 38, 127
AvaI CYCGRG 1 cut(s) 59
BbvI GCAGC 1 cut(s) 114
BccI CCATC 1 cut(s) 112
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 498
BcnI CCSGG 1 cut(s) 222
BfoI RGCGCY 2 cut(s) 212, 268
BisI GCNGC 3 cut(s) 11, 80, 128
BlsI GCNGC 3 cut(s) 12, 81, 129
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 2 cut(s) 222, 498
BmeT110I CYCGRG 1 cut(s) 59
BmiI GGNNCC 2 cut(s) 69, 78
BmrFI CCNGG 2 cut(s) 222, 498
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse1I ACTGG 2 cut(s) 148, 557
BseBI CCWGG 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 247, 296
BseNI ACTGG 2 cut(s) 148, 557
BseXI GCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 2 cut(s) 204, 221
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 471
BsoBI CYCGRG 1 cut(s) 59
Bsp143I GATC 6 cut(s) 299, 318, 362, 390, 402, 493
BspACI CCGC 4 cut(s) 11, 35, 80, 113
BspCNI CTCAG 2 cut(s) 246, 297
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 2 cut(s) 69, 78
BspPI GGATC 4 cut(s) 307, 357, 410, 488
BsrI ACTGG 2 cut(s) 148, 557
BssMI GATC 6 cut(s) 299, 318, 362, 390, 402, 493
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 2 cut(s) 347, 471
BstC8I GCNNGC 2 cut(s) 6, 432
BstDEI CTNAG 2 cut(s) 233, 305
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 2 cut(s) 212, 268
BstHHI GCGC 3 cut(s) 10, 211, 267
BstKTI GATC 6 cut(s) 302, 321, 365, 393, 405, 496
BstMBI GATC 6 cut(s) 299, 318, 362, 390, 402, 493
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 2 cut(s) 480, 484
BstSCI CCNGG 2 cut(s) 220, 496
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 299
BstYI RGATCY 1 cut(s) 299
Cac8I GCNNGC 2 cut(s) 6, 432
CaiI CAGNNNCTG 1 cut(s) 450
CfoI GCGC 3 cut(s) 10, 211, 267
Csp6I GTAC 4 cut(s) 151, 230, 501, 515
CviAII CATG 3 cut(s) 255, 477, 481
CviJI RGCY 4 cut(s) 79, 100, 168, 449
CviKI_1 RGCY 4 cut(s) 79, 100, 168, 449
CviQI GTAC 4 cut(s) 151, 230, 501, 515
DdeI CTNAG 2 cut(s) 233, 305
DpnI GATC 6 cut(s) 301, 320, 364, 392, 404, 495
DpnII GATC 6 cut(s) 299, 318, 362, 390, 402, 493
Eco47III AGCGCT 1 cut(s) 210
Eco88I CYCGRG 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 496
EcoT22I ATGCAT 1 cut(s) 482
FaeI CATG 3 cut(s) 258, 480, 484
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 471
FatI CATG 3 cut(s) 254, 476, 480
FauI CCCGC 1 cut(s) 28
Fnu4HI GCNGC 3 cut(s) 11, 80, 128
Fsp4HI GCNGC 3 cut(s) 11, 80, 128
GlaI GCGC 3 cut(s) 9, 210, 266
GluI GCNGC 3 cut(s) 11, 80, 128
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 2 cut(s) 212, 268
HapII CCGG 2 cut(s) 204, 221
HhaI GCGC 3 cut(s) 10, 211, 267
Hin1II CATG 3 cut(s) 258, 480, 484
Hin6I GCGC 3 cut(s) 8, 209, 265
HinP1I GCGC 3 cut(s) 8, 209, 265
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 4 cut(s) 245, 251, 373, 439
HpaII CCGG 2 cut(s) 204, 221
HphI GGTGA 2 cut(s) 38, 127
Hpy166II GTNNAC 2 cut(s) 467, 474
Hpy188I TCNGA 5 cut(s) 72, 250, 306, 390, 423
Hpy188III TCNNGA 3 cut(s) 17, 331, 560
Hpy8I GTNNAC 2 cut(s) 467, 474
Hpy99I CGWCG 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 347, 471
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 3 cut(s) 354, 370, 480
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 233, 305
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 3 cut(s) 258, 480, 484
HspAI GCGC 3 cut(s) 8, 209, 265
Kzo9I GATC 6 cut(s) 299, 318, 362, 390, 402, 493
LmnI GCTCC 3 cut(s) 47, 76, 206
Lsp1109I GCAGC 1 cut(s) 114
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 440, 580
MalI GATC 6 cut(s) 301, 320, 364, 392, 404, 495
MboI GATC 6 cut(s) 299, 318, 362, 390, 402, 493
MboII GAAGA 2 cut(s) 130, 188
MflI RGATCY 1 cut(s) 299
MluCI AATT 1 cut(s) 564
MlyI GAGTC 4 cut(s) 245, 254, 382, 448
MmeI TCCRAC 3 cut(s) 25, 95, 273
MnlI CCTC 5 cut(s) 25, 57, 78, 255, 369
Mph1103I ATGCAT 1 cut(s) 482
MseI TTAA 1 cut(s) 533
MslI CAYNNNNRTG 1 cut(s) 481
MspI CCGG 2 cut(s) 204, 221
MspR9I CCNGG 2 cut(s) 222, 498
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 222
NdeII GATC 6 cut(s) 299, 318, 362, 390, 402, 493
NlaIII CATG 3 cut(s) 258, 480, 484
NlaIV GGNNCC 2 cut(s) 69, 78
NmuCI GTSAC 2 cut(s) 440, 580
NsiI ATGCAT 1 cut(s) 482
NspI RCATGY 2 cut(s) 480, 484
PfoI TCCNGGA 1 cut(s) 220
PkrI GCNGC 3 cut(s) 12, 81, 129
PleI GAGTC 4 cut(s) 245, 253, 381, 447
PpsI GAGTC 4 cut(s) 245, 253, 381, 447
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 2 cut(s) 69, 78
PstNI CAGNNNCTG 1 cut(s) 450
PsuI RGATCY 1 cut(s) 299
RsaI GTAC 4 cut(s) 152, 231, 502, 516
RsaNI GTAC 4 cut(s) 151, 230, 501, 515
RseI CAYNNNNRTG 1 cut(s) 481
SaqAI TTAA 1 cut(s) 533
SatI GCNGC 3 cut(s) 11, 80, 128
Sau3AI GATC 6 cut(s) 299, 318, 362, 390, 402, 493
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 4 cut(s) 245, 254, 382, 448
ScrFI CCNGG 2 cut(s) 222, 498
SetI ASST 6 cut(s) 102, 231, 239, 276, 361, 502
SmiMI CAYNNNNRTG 1 cut(s) 481
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 1 cut(s) 564
SsiI CCGC 4 cut(s) 11, 35, 80, 113
StyD4I CCNGG 2 cut(s) 220, 496
TaaI ACNGT 2 cut(s) 347, 471
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 243, 361
TasI AATT 1 cut(s) 564
TatI WGTACW 1 cut(s) 150
TauI GCSGC 2 cut(s) 13, 82
Tru1I TTAA 1 cut(s) 533
Tru9I TTAA 1 cut(s) 533
TseFI GTSAC 2 cut(s) 440, 580
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 2 cut(s) 440, 580
TspDTI ATGAA 2 cut(s) 83, 524
TspGWI ACGGA 2 cut(s) 89, 355
XceI RCATGY 2 cut(s) 480, 484
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.