Rh2DG497800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002407
Physical Location & Seq
Reverse (-)
71798411 .. 71798782
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG497800.1

Sequence Viewer

Length: 372 bp
ATGCTCGACCCGAATTTGACCTTGGTGGAGCCCCAAATACACCAGCATCACCGGCCCGGACTTTTGCTCGACCTCACACAGTCCCTTAGGGTTTTCCACGGTCACAATTGTATCAAATGGCTTCTAATTGTTGAAATTGAAGACGACGATCTTGTGGAGAAGAACAACTACGATTTGGTATTGGTGGAGCCTGACCAACTTGACCTCAGACCGGAACGAGCGCTCACTGACGCAGCTGGACTGGCAATCTTCATAGGTCATGACCTTATTGGGAGGGCAGAACGAGGAAGCCGCCATTTGTTTTTGATCGGAGCAATGACGGGGGCTCTGGGGCCCTGGTGGTGGAGGAGAGGATTCTTGGATCTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

14.03

Weight (kDa)

5.34

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 292
AclWI GGATC 1 cut(s) 369
AcsI RAATTY 1 cut(s) 13
AfeI AGCGCT 1 cut(s) 222
AfiI CCNNNNNNNGG 2 cut(s) 211, 342
AgsI TTSAA 2 cut(s) 134, 140
AjnI CCWGG 1 cut(s) 335
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
AlwI GGATC 1 cut(s) 369
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 2 cut(s) 53, 332
ApaI GGGCCC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 13
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 54, 332, 333
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 41
AxyI CCTNAGG 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 336
BaeI ACNNNNGTAYC 2 cut(s) 94, 127
BanII GRGCYC 3 cut(s) 33, 328, 336
BarI GAAGNNNNNNTAC 2 cut(s) 152, 184
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 337
BcnI CCSGG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 224
BisI GCNGC 2 cut(s) 234, 292
BlsI GCNGC 2 cut(s) 235, 293
Bme1390I CCNGG 2 cut(s) 57, 337
BmgT120I GGNCC 3 cut(s) 54, 332, 333
BmiI GGNNCC 4 cut(s) 30, 189, 333, 334
BmrFI CCNGG 2 cut(s) 57, 337
BmsI GCATC 1 cut(s) 55
BpiI GAAGAC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 57
BsaJI CCNNGG 3 cut(s) 21, 97, 335
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 342
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 246
Bse21I CCTNAGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 21, 97, 335
BseLI CCNNNNNNNGG 2 cut(s) 211, 342
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 245
BshFI GGCC 2 cut(s) 55, 334
BsiSI CCGG 3 cut(s) 52, 57, 212
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 2 cut(s) 211, 342
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 2 cut(s) 55, 334
Bsp120I GGGCCC 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 33, 328, 336
Bsp143I GATC 3 cut(s) 148, 306, 361
BspACI CCGC 1 cut(s) 292
BspANI GGCC 2 cut(s) 55, 334
BspCNI CTCAG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 4 cut(s) 30, 189, 333, 334
BspPI GGATC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 21, 97, 335
BssMI GATC 3 cut(s) 148, 306, 361
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 81, 101
BstDEI CTNAG 2 cut(s) 86, 206
BstDSI CCRYGG 1 cut(s) 97
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 3 cut(s) 151, 309, 364
BstMBI GATC 3 cut(s) 148, 306, 361
BstMWI GCNNNNNNNGC 2 cut(s) 52, 242
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 2 cut(s) 55, 335
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
Bsu36I CCTNAGG 1 cut(s) 86
BsuRI GGCC 2 cut(s) 55, 334
BtgI CCRYGG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 225
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 223
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 3 cut(s) 54, 332, 333
CseI GACGC 1 cut(s) 239
CviAII CATG 1 cut(s) 260
CviJI RGCY 8 cut(s) 31, 55, 121, 190, 236, 291, 326, 334
CviKI_1 RGCY 8 cut(s) 31, 55, 121, 190, 236, 291, 326, 334
DdeI CTNAG 2 cut(s) 86, 206
DpnI GATC 3 cut(s) 150, 308, 363
DpnII GATC 3 cut(s) 148, 306, 361
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 3 cut(s) 33, 328, 336
Eco47III AGCGCT 1 cut(s) 222
Eco81I CCTNAGG 1 cut(s) 86
EcoO109I RGGNCCY 2 cut(s) 332, 333
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 3 cut(s) 33, 328, 336
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 263
FaiI YATR 2 cut(s) 254, 261
FaqI GGGAC 1 cut(s) 67
FatI CATG 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 234, 292
FriOI GRGCYC 3 cut(s) 33, 328, 336
Fsp4HI GCNGC 2 cut(s) 234, 292
GlaI GCGC 1 cut(s) 222
GluI GCNGC 2 cut(s) 234, 292
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 2 cut(s) 55, 334
HapII CCGG 3 cut(s) 52, 57, 212
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 223
Hin1II CATG 1 cut(s) 263
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 1 cut(s) 354
HpaII CCGG 3 cut(s) 52, 57, 212
HphI GGTGA 1 cut(s) 41
Hpy188I TCNGA 2 cut(s) 209, 311
Hpy188III TCNNGA 1 cut(s) 260
Hpy99I CGWCG 1 cut(s) 149
HpyCH4III ACNGT 2 cut(s) 81, 101
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 242
HpyF3I CTNAG 2 cut(s) 86, 206
Hsp92II CATG 1 cut(s) 263
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 3 cut(s) 148, 306, 361
LmnI GCTCC 3 cut(s) 28, 187, 311
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 55
MaeIII GTNAC 1 cut(s) 101
MalI GATC 3 cut(s) 150, 308, 363
MboI GATC 3 cut(s) 148, 306, 361
MboII GAAGA 3 cut(s) 152, 172, 241
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 3 cut(s) 33, 328, 336
MluCI AATT 4 cut(s) 13, 106, 126, 135
MnlI CCTC 6 cut(s) 83, 215, 267, 278, 339, 344
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 52, 57, 212
MspR9I CCNGG 2 cut(s) 57, 337
MunI CAATTG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 52, 242
NciI CCSGG 1 cut(s) 57
NdeII GATC 3 cut(s) 148, 306, 361
NlaIII CATG 1 cut(s) 263
NlaIV GGNNCC 4 cut(s) 30, 189, 333, 334
NmuCI GTSAC 1 cut(s) 101
PagI TCATGA 1 cut(s) 259
PfeI GAWTC 1 cut(s) 354
PkrI GCNGC 2 cut(s) 235, 293
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 4 cut(s) 30, 189, 333, 334
PspOMI GGGCCC 1 cut(s) 332
PspPI GGNCC 3 cut(s) 54, 332, 333
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 236
SatI GCNGC 2 cut(s) 234, 292
Sau3AI GATC 3 cut(s) 148, 306, 361
Sau96I GGNCC 3 cut(s) 54, 332, 333
ScrFI CCNGG 2 cut(s) 57, 337
SduI GDGCHC 3 cut(s) 33, 328, 336
SetI ASST 6 cut(s) 23, 75, 207, 238, 259, 267
SfaNI GCATC 1 cut(s) 55
Sse9I AATT 4 cut(s) 13, 106, 126, 135
SsiI CCGC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 55, 335
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 81, 101
TaqI TCGA 2 cut(s) 6, 69
TasI AATT 4 cut(s) 13, 106, 126, 135
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 1 cut(s) 354
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 241
TspRI CASTG 1 cut(s) 232
XapI RAATTY 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.