Rh2AG477000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002407
Physical Location & Seq
Reverse (-)
69631491 .. 69631931
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG477000.1

Sequence Viewer

Length: 441 bp
ATGATCTCCCAAAACCAAACCTCATCTTATCTCCAATCCAAGAATCCTCACCCAAATGGTCGACTGGTAATGCTCGACCCGAATTTGACCCTGGTGGAGCCCCAGATACACCAGCACCCCCGGCCCGGACTTTTGCTCGACCTCACACAGTCCCTGAGGGTTTTCCACGGTCACAATCGTATCAAACGGCTTCTAATCGTTGAAATTGAAGACGACGATCTTGTGGAGAAGAACAACTACGATTTGGTCTCGGTGGAGACTGACCGACTTGACCTCAGACCGGAACGAGCGCTGACTGACGCAGTGGGACTGGCGATCTTCATAGATCATGACCTTGTTGGGCGGGCAGAACGAGGAAGCCGCCATTTGTTTTTTATCAGAGCAATGACAGGGGCTTTGGGGCCATGGTGGTGGAGGAGAGGATTCTTGGATCTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.81

Weight (kDa)

6.36

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 61
AciI CCGC 2 cut(s) 343, 361
AclWI GGATC 1 cut(s) 438
AcsI RAATTY 1 cut(s) 82
AfeI AGCGCT 1 cut(s) 291
AfiI CCNNNNNNNGG 2 cut(s) 125, 280
AgsI TTSAA 2 cut(s) 203, 209
AjnI CCWGG 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 251, 253
AlwI GGATC 1 cut(s) 438
AlwNI CAGNNNCTG 1 cut(s) 154
Aor51HI AGCGCT 1 cut(s) 291
AoxI GGCC 2 cut(s) 122, 401
ApoI RAATTY 1 cut(s) 82
ArsI GACNNNNNNTTYG 2 cut(s) 379, 411
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 2 cut(s) 123, 401
AsuC2I CCSGG 2 cut(s) 121, 126
AsuHPI GGTGA 1 cut(s) 41
AxyI CCTNAGG 1 cut(s) 155
BaeI ACNNNNGTAYC 2 cut(s) 163, 196
BanII GRGCYC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbsI GAAGAC 1 cut(s) 216
BceAI ACGGC 1 cut(s) 203
BciT130I CCWGG 1 cut(s) 92
BcnI CCSGG 2 cut(s) 121, 126
BcoDI GTCTC 2 cut(s) 251, 253
BfoI RGCGCY 1 cut(s) 293
BisI GCNGC 1 cut(s) 361
BlsI GCNGC 1 cut(s) 362
Bme1390I CCNGG 3 cut(s) 92, 121, 126
BmgT120I GGNCC 2 cut(s) 123, 401
BmiI GGNNCC 2 cut(s) 99, 402
BmrFI CCNGG 3 cut(s) 92, 121, 126
BpiI GAAGAC 1 cut(s) 216
BpuMI CCSGG 2 cut(s) 121, 126
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 4 cut(s) 90, 119, 166, 404
BsaWI WCCGGW 1 cut(s) 280
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 280
Bse1I ACTGG 2 cut(s) 69, 315
Bse21I CCTNAGG 1 cut(s) 155
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 4 cut(s) 90, 119, 166, 404
BseLI CCNNNNNNNGG 2 cut(s) 125, 280
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 2 cut(s) 146, 289
BseNI ACTGG 2 cut(s) 69, 315
BseRI GAGGAG 1 cut(s) 430
BshFI GGCC 2 cut(s) 124, 403
BsiSI CCGG 3 cut(s) 121, 126, 281
BslFI GGGAC 2 cut(s) 136, 321
BslI CCNNNNNNNGG 2 cut(s) 125, 280
BsmAI GTCTC 2 cut(s) 251, 253
BsmFI GGGAC 2 cut(s) 136, 321
BsnI GGCC 2 cut(s) 124, 403
Bso31I GGTCTC 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 5 cut(s) 3, 217, 315, 325, 430
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 2 cut(s) 343, 361
BspANI GGCC 2 cut(s) 124, 403
BspCNI CTCAG 2 cut(s) 147, 288
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 99, 402
BspPI GGATC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 390
BsrI ACTGG 2 cut(s) 69, 315
BssECI CCNNGG 4 cut(s) 90, 119, 166, 404
BssMI GATC 5 cut(s) 3, 217, 315, 325, 430
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 150, 170
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 2 cut(s) 155, 275
BstDSI CCRYGG 2 cut(s) 166, 404
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 5 cut(s) 6, 220, 318, 328, 433
BstMAI GTCTC 2 cut(s) 251, 253
BstMBI GATC 5 cut(s) 3, 217, 315, 325, 430
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 3 cut(s) 90, 119, 124
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 430
BstXI CCANNNNNNTGG 1 cut(s) 411
BstYI RGATCY 1 cut(s) 430
Bsu36I CCTNAGG 1 cut(s) 155
BsuRI GGCC 2 cut(s) 124, 403
BtgI CCRYGG 2 cut(s) 166, 404
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 123, 401
CseI GACGC 1 cut(s) 308
CviAII CATG 2 cut(s) 329, 405
CviJI RGCY 6 cut(s) 100, 124, 190, 360, 395, 403
CviKI_1 RGCY 6 cut(s) 100, 124, 190, 360, 395, 403
DdeI CTNAG 2 cut(s) 155, 275
DpnI GATC 5 cut(s) 5, 219, 317, 327, 432
DpnII GATC 5 cut(s) 3, 217, 315, 325, 430
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 253
Eco47III AGCGCT 1 cut(s) 291
Eco81I CCTNAGG 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 332, 408
FaiI YATR 3 cut(s) 323, 330, 406
FaqI GGGAC 2 cut(s) 136, 321
FatI CATG 2 cut(s) 328, 404
FauI CCCGC 1 cut(s) 336
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 361
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 361
GlaI GCGC 1 cut(s) 291
GluI GCNGC 1 cut(s) 361
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 2 cut(s) 124, 403
HapII CCGG 3 cut(s) 121, 126, 281
HgaI GACGC 1 cut(s) 308
HhaI GCGC 1 cut(s) 292
Hin1II CATG 2 cut(s) 332, 408
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 62
HindII GTYRAC 1 cut(s) 62
HinfI GANTC 2 cut(s) 43, 423
HpaII CCGG 3 cut(s) 121, 126, 281
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 2 cut(s) 278, 380
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 1 cut(s) 62
Hpy99I CGWCG 1 cut(s) 218
HpyCH4III ACNGT 2 cut(s) 150, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 2 cut(s) 155, 275
Hsp92II CATG 2 cut(s) 332, 408
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 5 cut(s) 3, 217, 315, 325, 430
LmnI GCTCC 1 cut(s) 97
MaeIII GTNAC 1 cut(s) 170
MalI GATC 5 cut(s) 5, 219, 317, 327, 432
MboI GATC 5 cut(s) 3, 217, 315, 325, 430
MboII GAAGA 3 cut(s) 221, 241, 310
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 2 cut(s) 82, 204
MnlI CCTC 8 cut(s) 31, 57, 150, 152, 284, 347, 408, 413
MslI CAYNNNNRTG 2 cut(s) 54, 409
MspI CCGG 3 cut(s) 121, 126, 281
MspR9I CCNGG 3 cut(s) 92, 121, 126
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 121
NciI CCSGG 2 cut(s) 121, 126
NcoI CCATGG 1 cut(s) 404
NdeII GATC 5 cut(s) 3, 217, 315, 325, 430
NlaIII CATG 2 cut(s) 332, 408
NlaIV GGNNCC 2 cut(s) 99, 402
NmuCI GTSAC 1 cut(s) 170
PagI TCATGA 1 cut(s) 328
PfeI GAWTC 2 cut(s) 43, 423
PkrI GCNGC 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 99, 402
PspPI GGNCC 2 cut(s) 123, 401
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 430
RseI CAYNNNNRTG 2 cut(s) 54, 409
SalI GTCGAC 1 cut(s) 60
SatI GCNGC 1 cut(s) 361
Sau3AI GATC 5 cut(s) 3, 217, 315, 325, 430
Sau96I GGNCC 2 cut(s) 123, 401
ScrFI CCNGG 3 cut(s) 92, 121, 126
SduI GDGCHC 1 cut(s) 102
SetI ASST 4 cut(s) 23, 144, 276, 336
SmiMI CAYNNNNRTG 2 cut(s) 54, 409
Sse9I AATT 2 cut(s) 82, 204
SsiI CCGC 2 cut(s) 343, 361
StyD4I CCNGG 3 cut(s) 90, 119, 124
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 150, 170
TaqI TCGA 3 cut(s) 61, 75, 138
TaqII GACCGA 1 cut(s) 279
TasI AATT 2 cut(s) 82, 204
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 2 cut(s) 43, 423
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 310
TspRI CASTG 1 cut(s) 309
XapI RAATTY 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.