Rh3BG166900

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002407
Physical Location & Seq
Forward (+)
14370476 .. 14383736
13261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG166900.1

Sequence Viewer

Length: 837 bp
ATGGAGTTGATGAGGTTGAGTCCAGGAGCTTCTAACTCCAAGAGAGACTGCGAGTTTAGAGCTGGTAAGTTTCATGGAATAGGGAACAACTACGATTCGGTCGCGGCGGAGCCTGACCGACTTGACCTCGGACCGGAACTGAGGCTATGGGAGGCCAAACAAGGAAGCCGCCATTTGTTTTTGATCAGAGCAATGACGGGGGCTCTAGGGCTATGGTGGTGGAGGAGAGGATTCTTGGGTCTGGGTGGATTAAGATTGGGTCAGATTGATCGACCCCGATCAGACACATATACTGGATTCTTTATTGGCGGCGATGACAAGCACGAAGTGTGCTCGTTAGATGCAGATGACTTGGGTGGCTGGCTTAGCCATGGCAGATTGGGTCATTCTGATGGTGAGCAAAGAGACCGATTGATCTGCAAGATCACTTCCAATGCACTAATCCTTTCCCAAGATCCATTTGGGAACTATGTCGTGCAATTCGTCTTTGAGCTTCAGCTTCCGTGGGCGACAGTAGACATTCTTGAGCAGTTGGAGGGTAATTATGGGGATTTGTCTGTGGAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGCCGTGCTCGCATTATTCAGGAACTGATAGGGAATATGCGATTGGACCAAATCATGCAAGACCCTTATGGCAATTATGTTATCCAAGCAGCACTCAGTCAATCAAAGGGAACACTTCATTCTAAACTGTCCCTATGGGAAGAAAATCCTCTCTACCAATATTTTGATGAAATAATCTCTTTTATTGGTTTAATTGTTCAAGAGAATTCGATTGCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF zf-CCCH

Protein Analysis

278

Amino Acids

31.47

Weight (kDa)

5.23

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 182 - 215 4.2e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 104
AciI CCGC 4 cut(s) 104, 107, 169, 309
AclWI GGATC 1 cut(s) 449
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 1 cut(s) 479
AdeI CACNNNGTG 1 cut(s) 328
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 2 cut(s) 587, 815
AjnI CCWGG 1 cut(s) 22
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 4 cut(s) 29, 62, 493, 499
AluI AGCT 4 cut(s) 29, 62, 493, 499
Alw21I GWGCWC 2 cut(s) 335, 625
Alw26I GTCTC 2 cut(s) 39, 399
AlwI GGATC 1 cut(s) 449
AlwNI CAGNNNCTG 1 cut(s) 640
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 704
ApoI RAATTY 1 cut(s) 820
AspLEI GCGC 1 cut(s) 618
AspS9I GGNCC 2 cut(s) 131, 661
AsuHPI GGTGA 2 cut(s) 407, 620
AvaII GGWCC 2 cut(s) 131, 661
BanII GRGCYC 1 cut(s) 205
Bbv12I GWGCWC 2 cut(s) 335, 625
BbvI GCAGC 1 cut(s) 716
BccI CCATC 1 cut(s) 386
BceAI ACGGC 1 cut(s) 603
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 1 cut(s) 24
BclI TGATCA 1 cut(s) 183
BcoDI GTCTC 2 cut(s) 39, 399
BfaI CTAG 1 cut(s) 206
BfoI RGCGCY 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 596
BisI GCNGC 4 cut(s) 105, 169, 310, 705
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 4 cut(s) 106, 170, 311, 706
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 2 cut(s) 131, 661
BmgT120I GGNCC 2 cut(s) 131, 661
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 1 cut(s) 331
Bpu1102I GCTNAGC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 127, 370, 503
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 127, 370, 503
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 2 cut(s) 131, 724
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 716
Bsh1236I CGCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 2 cut(s) 335, 625
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 730
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 2 cut(s) 39, 399
BsmFI GGGAC 1 cut(s) 730
BsnI GGCC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 399
Bsp1286I GDGCHC 3 cut(s) 205, 335, 625
Bsp143I GATC 6 cut(s) 183, 268, 278, 414, 423, 454
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 370
BspACI CCGC 4 cut(s) 104, 107, 169, 309
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 2 cut(s) 132, 723
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 1 cut(s) 449
BspQI GCTCTTC 1 cut(s) 606
BspTNI GGTCTC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 3 cut(s) 127, 370, 503
BssMI GATC 6 cut(s) 183, 268, 278, 414, 423, 454
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 514, 744
Bst6I CTCTTC 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 362, 625
BstDEI CTNAG 3 cut(s) 140, 365, 710
BstDSI CCRYGG 2 cut(s) 370, 503
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 1 cut(s) 618
BstKTI GATC 6 cut(s) 186, 271, 281, 417, 426, 457
BstMAI GTCTC 2 cut(s) 39, 399
BstMBI GATC 6 cut(s) 183, 268, 278, 414, 423, 454
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 366, 624
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 1 cut(s) 716
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 2 cut(s) 370, 503
BtgZI GCGATG 1 cut(s) 327
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 2 cut(s) 362, 625
CaiI CAGNNNCTG 1 cut(s) 640
CfoI GCGC 1 cut(s) 618
Cfr13I GGNCC 2 cut(s) 131, 661
CpoI CGGWCCG 1 cut(s) 131
CspI CGGWCCG 1 cut(s) 131
CviAII CATG 3 cut(s) 74, 371, 670
DdeI CTNAG 3 cut(s) 140, 365, 710
DpnI GATC 6 cut(s) 185, 270, 280, 416, 425, 456
DpnII GATC 6 cut(s) 183, 268, 278, 414, 423, 454
DraIII CACNNNGTG 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 131, 661
Eco57I CTGAAG 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 3 cut(s) 77, 374, 673
FaqI GGGAC 1 cut(s) 730
FatI CATG 3 cut(s) 73, 370, 669
FbaI TGATCA 1 cut(s) 183
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 4 cut(s) 105, 169, 310, 705
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 4 cut(s) 105, 169, 310, 705
FspBI CTAG 1 cut(s) 206
GlaI GCGC 1 cut(s) 617
GluI GCNGC 4 cut(s) 105, 169, 310, 705
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 1 cut(s) 155
HapII CCGG 1 cut(s) 134
HhaI GCGC 1 cut(s) 618
Hin1II CATG 3 cut(s) 77, 374, 673
Hin6I GCGC 1 cut(s) 616
HinP1I GCGC 1 cut(s) 616
HinfI GANTC 4 cut(s) 19, 95, 231, 297
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 407, 620
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 5 cut(s) 131, 188, 264, 283, 391
Hpy188III TCNNGA 3 cut(s) 524, 635, 815
Hpy8I GTNNAC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 514, 744
HpyCH4V TGCA 6 cut(s) 344, 420, 437, 478, 673, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 366, 624
HpyF3I CTNAG 3 cut(s) 140, 365, 710
Hsp92II CATG 3 cut(s) 77, 374, 673
HspAI GCGC 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 183
Kzo9I GATC 6 cut(s) 183, 268, 278, 414, 423, 454
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 2 cut(s) 26, 109
Lsp1109I GCAGC 1 cut(s) 716
LweI GCATC 1 cut(s) 331
MaeI CTAG 1 cut(s) 206
MalI GATC 6 cut(s) 185, 270, 280, 416, 425, 456
MboI GATC 6 cut(s) 183, 268, 278, 414, 423, 454
MboII GAAGA 2 cut(s) 623, 767
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 3 cut(s) 205, 335, 625
MluCI AATT 5 cut(s) 479, 541, 688, 807, 820
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 8 cut(s) 6, 135, 137, 145, 216, 221, 529, 774
MseI TTAA 3 cut(s) 251, 806, 835
MslI CAYNNNNRTG 1 cut(s) 390
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 24
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 366, 624
NcoI CCATGG 1 cut(s) 370
NdeII GATC 6 cut(s) 183, 268, 278, 414, 423, 454
NlaIII CATG 3 cut(s) 77, 374, 673
NlaIV GGNNCC 1 cut(s) 111
PaqCI CACCTGC 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 606
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PfeI GAWTC 3 cut(s) 95, 231, 297
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 4 cut(s) 106, 170, 311, 706
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 131, 661
PstNI CAGNNNCTG 1 cut(s) 640
PsuI RGATCY 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 390
Rsr2I CGGWCCG 1 cut(s) 131
RsrII CGGWCCG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 3 cut(s) 251, 806, 835
SatI GCNGC 4 cut(s) 105, 169, 310, 705
Sau3AI GATC 6 cut(s) 183, 268, 278, 414, 423, 454
Sau96I GGNCC 2 cut(s) 131, 661
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 3 cut(s) 205, 335, 625
SetI ASST 7 cut(s) 17, 31, 64, 129, 495, 501, 610
SfaNI GCATC 1 cut(s) 331
SinI GGWCC 2 cut(s) 131, 661
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 5 cut(s) 479, 541, 688, 807, 820
SsiI CCGC 4 cut(s) 104, 107, 169, 309
SspI AATATT 1 cut(s) 776
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 2 cut(s) 514, 744
TaqI TCGA 2 cut(s) 271, 824
TaqII GACCGA 3 cut(s) 88, 132, 423
TasI AATT 5 cut(s) 479, 541, 688, 807, 820
TauI GCSGC 3 cut(s) 107, 171, 312
TfiI GAWTC 3 cut(s) 95, 231, 297
Tru1I TTAA 3 cut(s) 251, 806, 835
Tru9I TTAA 3 cut(s) 251, 806, 835
TseI GCWGC 1 cut(s) 704
TspDTI ATGAA 3 cut(s) 62, 722, 798
TspGWI ACGGA 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 131, 661
XapI RAATTY 1 cut(s) 820
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.