Rh4BG080300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002407
Physical Location & Seq
Forward (+)
14002714 .. 14003178
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG080300.1

Sequence Viewer

Length: 465 bp
ATGATCCAGTACGGTCACTTAGCTGACGGTGTGGACTTCGACAGGTGGAGGTGGAGAAAGCGTTTGATTTGGGTTGAAATGGAGTTGATGAGGTTGAGTCCAGGAGCTTCTAACTCCAAGAGAGACTGCGAGTTCAGAGCTGGTAAGTTTCATGGAATCGGGAACAACTACGATTCGGTCGCGACGGAGCCTAACCGACTTGACCTCGGACCGGAACTGGGGCTATGGGAGGCCAAACGAGGAAGCCGCCATTTGTTTTTTATCGGAGCAATGACGGGGGCTCTGGGGCTATGGTGGTGGAGGAGAGGATTCTTGGATCTGGGTGGATTAAGATCGGGTCAGATTGATCGACCCCGATCGGACACACATACTGGATTCTCTATTGGCGGCGACGACAAGCACGAAGTGTGCTCGTTAGATGTAGATGACTTGGGTGGCTGGCTTAGCCATGGCAGATTGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.47

Weight (kDa)

7.02

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 182
AciI CCGC 2 cut(s) 247, 387
AclWI GGATC 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 211, 218
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 3 cut(s) 23, 107, 140
AluI AGCT 3 cut(s) 23, 107, 140
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 1 cut(s) 324
AoxI GGCC 1 cut(s) 231
AspS9I GGNCC 1 cut(s) 209
AvaII GGWCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 413
BciT130I CCWGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 117
BisI GCNGC 2 cut(s) 247, 388
BlpI GCTNAGC 1 cut(s) 443
BlsI GCNGC 2 cut(s) 248, 389
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 102
BmrI ACTGGG 1 cut(s) 227
BmuI ACTGGG 1 cut(s) 227
Bpu1102I GCTNAGC 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 205, 448
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 218
Bse1I ACTGG 3 cut(s) 7, 222, 376
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 331
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 205, 448
BseJI GATNNNNATC 1 cut(s) 331
BseLI CCNNNNNNNGG 2 cut(s) 211, 218
BseMI GCAATG 1 cut(s) 276
BseNI ACTGG 3 cut(s) 7, 222, 376
BseRI GAGGAG 1 cut(s) 316
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 2 cut(s) 180, 359
BshFI GGCC 1 cut(s) 233
BsiEI CGRYCG 2 cut(s) 180, 359
BsiHKAI GWGCWC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 211, 218
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 283, 413
Bsp143I GATC 5 cut(s) 3, 316, 332, 346, 356
Bsp1720I GCTNAGC 1 cut(s) 443
Bsp19I CCATGG 1 cut(s) 448
Bsp68I TCGCGA 1 cut(s) 182
BspACI CCGC 2 cut(s) 247, 387
BspANI GGCC 1 cut(s) 233
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 3 cut(s) 7, 222, 376
BssECI CCNNGG 2 cut(s) 205, 448
BssMI GATC 5 cut(s) 3, 316, 332, 346, 356
BssT1I CCWWGG 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 14, 29
BstC8I GCNNGC 1 cut(s) 440
BstDEI CTNAG 2 cut(s) 19, 443
BstDSI CCRYGG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 5 cut(s) 6, 319, 335, 349, 359
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 5 cut(s) 3, 316, 332, 346, 356
BstMCI CGRYCG 2 cut(s) 180, 359
BstMWI GCNNNNNNNGC 1 cut(s) 444
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 100
BstUI CGCG 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BsuRI GGCC 1 cut(s) 233
BtgI CCRYGG 1 cut(s) 448
BtuMI TCGCGA 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 209
CpoI CGGWCCG 1 cut(s) 209
Csp6I GTAC 1 cut(s) 10
CspI CGGWCCG 1 cut(s) 209
CviAII CATG 2 cut(s) 152, 449
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 19, 443
DpnI GATC 5 cut(s) 5, 318, 334, 348, 358
DpnII GATC 5 cut(s) 3, 316, 332, 346, 356
DraIII CACNNNGTG 1 cut(s) 406
Eco130I CCWWGG 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 2 cut(s) 155, 452
FaiI YATR 5 cut(s) 153, 226, 292, 369, 450
FatI CATG 2 cut(s) 151, 448
Fnu4HI GCNGC 2 cut(s) 247, 388
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 2 cut(s) 247, 388
GluI GCNGC 2 cut(s) 247, 388
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 212
Hin1II CATG 2 cut(s) 155, 452
HinfI GANTC 5 cut(s) 97, 156, 173, 309, 375
HpaII CCGG 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 5 cut(s) 137, 209, 266, 342, 361
Hpy188III TCNNGA 2 cut(s) 160, 181
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 2 cut(s) 187, 395
HpyCH4III ACNGT 2 cut(s) 14, 29
HpyF10VI GCNNNNNNNGC 1 cut(s) 444
HpyF3I CTNAG 2 cut(s) 19, 443
Hsp92II CATG 2 cut(s) 155, 452
Kzo9I GATC 5 cut(s) 3, 316, 332, 346, 356
LmnI GCTCC 3 cut(s) 104, 187, 266
MaeIII GTNAC 1 cut(s) 14
MalI GATC 5 cut(s) 5, 318, 334, 348, 358
MboI GATC 5 cut(s) 3, 316, 332, 346, 356
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 283, 413
MlyI GAGTC 1 cut(s) 106
MnlI CCTC 7 cut(s) 42, 84, 215, 223, 233, 294, 299
MseI TTAA 1 cut(s) 329
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 102
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 444
NcoI CCATGG 1 cut(s) 448
NdeII GATC 5 cut(s) 3, 316, 332, 346, 356
NlaIII CATG 2 cut(s) 155, 452
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 14
NruI TCGCGA 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PfeI GAWTC 4 cut(s) 156, 173, 309, 375
PfoI TCCNGGA 1 cut(s) 100
PkrI GCNGC 2 cut(s) 248, 389
Ple19I CGATCG 1 cut(s) 359
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 1 cut(s) 316
PvuI CGATCG 1 cut(s) 359
RruI TCGCGA 1 cut(s) 182
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
Rsr2I CGGWCCG 1 cut(s) 209
RsrII CGGWCCG 1 cut(s) 209
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 2 cut(s) 247, 388
Sau3AI GATC 5 cut(s) 3, 316, 332, 346, 356
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 2 cut(s) 283, 413
SetI ASST 7 cut(s) 25, 47, 53, 95, 109, 142, 207
SinI GGWCC 1 cut(s) 209
SsiI CCGC 2 cut(s) 247, 387
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 2 cut(s) 14, 29
TaqI TCGA 2 cut(s) 39, 349
TaqII GACCGA 1 cut(s) 166
TauI GCSGC 2 cut(s) 249, 390
TfiI GAWTC 4 cut(s) 156, 173, 309, 375
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TseFI GTSAC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 140
TspGWI ACGGA 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.