Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1480 |
| AccBSI | CCGCTC | 1 cut(s) | 1301 |
| AccII | CGCG | 3 cut(s) | 130, 144, 1268 |
| AccIII | TCCGGA | 1 cut(s) | 853 |
| AciI | CCGC | 3 cut(s) | 560, 1266, 1301 |
| AclWI | GGATC | 3 cut(s) | 151, 955, 961 |
| AcyI | GRCGYC | 1 cut(s) | 111 |
| AdeI | CACNNNGTG | 2 cut(s) | 760, 1090 |
| AfaI | GTAC | 4 cut(s) | 893, 1013, 1047, 1444 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 475, 668, 669, 1480 |
| AflIII | ACRYGT | 1 cut(s) | 128 |
| AgeI | ACCGGT | 1 cut(s) | 1445 |
| AgsI | TTSAA | 4 cut(s) | 302, 438, 1327, 1370 |
| AjnI | CCWGG | 2 cut(s) | 894, 1159 |
| AluBI | AGCT | 9 cut(s) | 29, 37, 272, 767, 773, 820, 1031, 1134, 1408 |
| AluI | AGCT | 9 cut(s) | 29, 37, 272, 767, 773, 820, 1031, 1134, 1408 |
| Alw21I | GWGCWC | 3 cut(s) | 246, 345, 756 |
| Alw26I | GTCTC | 6 cut(s) | 121, 137, 193, 397, 605, 1024 |
| Alw44I | GTGCAC | 1 cut(s) | 242 |
| AlwI | GGATC | 3 cut(s) | 151, 955, 961 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 680 |
| Ama87I | CYCGRG | 1 cut(s) | 1433 |
| Aor13HI | TCCGGA | 1 cut(s) | 853 |
| ApaLI | GTGCAC | 1 cut(s) | 242 |
| ApeKI | GCWGC | 6 cut(s) | 206, 209, 218, 713, 1031, 1451 |
| AsiGI | ACCGGT | 1 cut(s) | 1445 |
| AspLEI | GCGC | 1 cut(s) | 144 |
| AspS9I | GGNCC | 1 cut(s) | 371 |
| AsuHPI | GGTGA | 4 cut(s) | 490, 620, 895, 1132 |
| AsuII | TTCGAA | 1 cut(s) | 12 |
| AvaI | CYCGRG | 1 cut(s) | 1433 |
| AvaII | GGWCC | 1 cut(s) | 371 |
| BaeGI | GKGCMC | 1 cut(s) | 246 |
| BauI | CACGAG | 1 cut(s) | 1403 |
| Bbv12I | GWGCWC | 3 cut(s) | 246, 345, 756 |
| BbvI | GCAGC | 6 cut(s) | 193, 196, 205, 700, 1018, 1463 |
| BccI | CCATC | 6 cut(s) | 11, 172, 697, 920, 1055, 1120 |
| BceAI | ACGGC | 4 cut(s) | 98, 968, 992, 1337 |
| BciT130I | CCWGG | 2 cut(s) | 896, 1161 |
| BciVI | GTATCC | 2 cut(s) | 44, 861 |
| BclI | TGATCA | 2 cut(s) | 828, 909 |
| BcoDI | GTCTC | 6 cut(s) | 121, 137, 193, 397, 605, 1024 |
| BfaI | CTAG | 3 cut(s) | 749, 1028, 1100 |
| BfmI | CTRYAG | 1 cut(s) | 717 |
| BfuI | GTATCC | 2 cut(s) | 44, 861 |
| BglII | AGATCT | 1 cut(s) | 1372 |
| BisI | GCNGC | 6 cut(s) | 207, 210, 219, 714, 1032, 1452 |
| BlpI | GCTNAGC | 1 cut(s) | 1035 |
| BlsI | GCNGC | 6 cut(s) | 208, 211, 220, 715, 1033, 1453 |
| Bme1390I | CCNGG | 2 cut(s) | 896, 1161 |
| Bme18I | GGWCC | 1 cut(s) | 371 |
| BmeT110I | CYCGRG | 1 cut(s) | 1433 |
| BmgT120I | GGNCC | 1 cut(s) | 371 |
| BmiI | GGNNCC | 2 cut(s) | 1158, 1165 |
| BmrFI | CCNGG | 2 cut(s) | 896, 1161 |
| BmsI | GCATC | 3 cut(s) | 310, 1077, 1121 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1483 |
| BpmI | CTGGAG | 3 cut(s) | 617, 789, 1182 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1035 |
| Bpu14I | TTCGAA | 1 cut(s) | 12 |
| BpuEI | CTTGAG | 3 cut(s) | 252, 842, 1259 |
| Bsa29I | ATCGAT | 1 cut(s) | 261 |
| BsaAI | YACGTR | 1 cut(s) | 849 |
| BsaBI | GATNNNNATC | 2 cut(s) | 540, 952 |
| BsaHI | GRCGYC | 1 cut(s) | 111 |
| BsaJI | CCNNGG | 3 cut(s) | 530, 1152, 1474 |
| BsaWI | WCCGGW | 2 cut(s) | 853, 1445 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 762, 792, 1141, 1171 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 475, 668, 669, 1480 |
| Bse118I | RCCGGY | 1 cut(s) | 1445 |
| Bse1I | ACTGG | 1 cut(s) | 1485 |
| Bse3DI | GCAATG | 2 cut(s) | 219, 1233 |
| Bse8I | GATNNNNATC | 2 cut(s) | 540, 952 |
| BseAI | TCCGGA | 1 cut(s) | 853 |
| BseBI | CCWGG | 2 cut(s) | 896, 1161 |
| BseCI | ATCGAT | 1 cut(s) | 261 |
| BseDI | CCNNGG | 3 cut(s) | 530, 1152, 1474 |
| BseGI | GGATG | 2 cut(s) | 708, 868 |
| BseJI | GATNNNNATC | 2 cut(s) | 540, 952 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 475, 668, 669, 1480 |
| BseMI | GCAATG | 2 cut(s) | 219, 1233 |
| BseMII | CTCAG | 5 cut(s) | 148, 891, 1049, 1092, 1332 |
| BseNI | ACTGG | 1 cut(s) | 1485 |
| BseSI | GKGCMC | 1 cut(s) | 246 |
| BseXI | GCAGC | 6 cut(s) | 193, 196, 205, 700, 1018, 1463 |
| BsgI | GTGCAG | 2 cut(s) | 708, 1470 |
| Bsh1236I | CGCG | 3 cut(s) | 130, 144, 1268 |
| BshTI | ACCGGT | 1 cut(s) | 1445 |
| BshVI | ATCGAT | 1 cut(s) | 261 |
| BsiHKAI | GWGCWC | 3 cut(s) | 246, 345, 756 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1433 |
| BsiSI | CCGG | 2 cut(s) | 854, 1446 |
| BslFI | GGGAC | 2 cut(s) | 131, 736 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 475, 668, 669, 1480 |
| BsmAI | GTCTC | 6 cut(s) | 121, 137, 193, 397, 605, 1024 |
| BsmBI | CGTCTC | 2 cut(s) | 121, 137 |
| BsmFI | GGGAC | 2 cut(s) | 131, 736 |
| BsoBI | CYCGRG | 1 cut(s) | 1433 |
| Bsp119I | TTCGAA | 1 cut(s) | 12 |
| Bsp1286I | GDGCHC | 3 cut(s) | 246, 345, 756 |
| Bsp13I | TCCGGA | 1 cut(s) | 853 |
| Bsp1407I | TGTACA | 1 cut(s) | 1011 |
| Bsp143I | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1035 |
| Bsp19I | CCATGG | 2 cut(s) | 530, 1474 |
| BspACI | CCGC | 3 cut(s) | 560, 1266, 1301 |
| BspCNI | CTCAG | 5 cut(s) | 147, 892, 1048, 1091, 1331 |
| BspDI | ATCGAT | 1 cut(s) | 261 |
| BspEI | TCCGGA | 1 cut(s) | 853 |
| BspFNI | CGCG | 3 cut(s) | 130, 144, 1268 |
| BspLI | GGNNCC | 2 cut(s) | 1158, 1165 |
| BspPI | GGATC | 3 cut(s) | 151, 955, 961 |
| BspT104I | TTCGAA | 1 cut(s) | 12 |
| BsrBI | CCGCTC | 1 cut(s) | 1301 |
| BsrDI | GCAATG | 2 cut(s) | 219, 1233 |
| BsrFI | RCCGGY | 1 cut(s) | 1445 |
| BsrGI | TGTACA | 1 cut(s) | 1011 |
| BsrI | ACTGG | 1 cut(s) | 1485 |
| BssAI | RCCGGY | 1 cut(s) | 1445 |
| BssECI | CCNNGG | 3 cut(s) | 530, 1152, 1474 |
| BssMI | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| BssNI | GRCGYC | 1 cut(s) | 111 |
| BssSI | CACGAG | 1 cut(s) | 1403 |
| BssT1I | CCWWGG | 2 cut(s) | 530, 1474 |
| Bst2BI | CACGAG | 1 cut(s) | 1403 |
| Bst2UI | CCWGG | 2 cut(s) | 896, 1161 |
| Bst4CI | ACNGT | 6 cut(s) | 187, 616, 682, 1043, 1120, 1184 |
| Bst6I | CTCTTC | 1 cut(s) | 494 |
| BstACI | GRCGYC | 1 cut(s) | 111 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 218 |
| BstAUI | TGTACA | 1 cut(s) | 1011 |
| BstBAI | YACGTR | 1 cut(s) | 849 |
| BstBI | TTCGAA | 1 cut(s) | 12 |
| BstC8I | GCNNGC | 2 cut(s) | 140, 1350 |
| BstDEI | CTNAG | 7 cut(s) | 134, 757, 900, 1035, 1078, 1318, 1427 |
| BstDSI | CCRYGG | 2 cut(s) | 530, 1474 |
| BstF5I | GGATG | 2 cut(s) | 708, 868 |
| BstFNI | CGCG | 3 cut(s) | 130, 144, 1268 |
| BstHHI | GCGC | 1 cut(s) | 144 |
| BstKTI | GATC | 7 cut(s) | 159, 544, 831, 912, 950, 956, 1375 |
| BstMAI | GTCTC | 6 cut(s) | 121, 137, 193, 397, 605, 1024 |
| BstMBI | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 127, 215, 218, 320, 1163 |
| BstNI | CCWGG | 2 cut(s) | 896, 1161 |
| BstNSI | RCATGY | 1 cut(s) | 1352 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1483 |
| BstSCI | CCNGG | 2 cut(s) | 894, 1159 |
| BstSFI | CTRYAG | 1 cut(s) | 717 |
| BstSLI | GKGCMC | 1 cut(s) | 246 |
| BstSNI | TACGTA | 1 cut(s) | 849 |
| BstUI | CGCG | 3 cut(s) | 130, 144, 1268 |
| BstV1I | GCAGC | 6 cut(s) | 193, 196, 205, 700, 1018, 1463 |
| BstX2I | RGATCY | 3 cut(s) | 947, 953, 1372 |
| BstYI | RGATCY | 3 cut(s) | 947, 953, 1372 |
| Bsu15I | ATCGAT | 1 cut(s) | 261 |
| BsuI | GTATCC | 2 cut(s) | 44, 861 |
| BsuTUI | ATCGAT | 1 cut(s) | 261 |
| BtgI | CCRYGG | 2 cut(s) | 530, 1474 |
| BtsCI | GGATG | 2 cut(s) | 708, 868 |
| BtsIMutI | CAGTG | 3 cut(s) | 105, 183, 1326 |
| Cac8I | GCNNGC | 2 cut(s) | 140, 1350 |
| CaiI | CAGNNNCTG | 1 cut(s) | 680 |
| CfoI | GCGC | 1 cut(s) | 144 |
| Cfr10I | RCCGGY | 1 cut(s) | 1445 |
| Cfr13I | GGNCC | 1 cut(s) | 371 |
| ClaI | ATCGAT | 1 cut(s) | 261 |
| CseI | GACGC | 5 cut(s) | 119, 119, 133, 154, 303 |
| Csp6I | GTAC | 4 cut(s) | 892, 1012, 1046, 1443 |
| CspAI | ACCGGT | 1 cut(s) | 1445 |
| CviAII | CATG | 6 cut(s) | 162, 202, 496, 531, 1349, 1475 |
| CviQI | GTAC | 4 cut(s) | 892, 1012, 1046, 1443 |
| DdeI | CTNAG | 7 cut(s) | 134, 757, 900, 1035, 1078, 1318, 1427 |
| DpnI | GATC | 7 cut(s) | 158, 543, 830, 911, 949, 955, 1374 |
| DpnII | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| DraI | TTTAAA | 1 cut(s) | 331 |
| DraIII | CACNNNGTG | 2 cut(s) | 760, 1090 |
| Eam1104I | CTCTTC | 1 cut(s) | 494 |
| EarI | CTCTTC | 1 cut(s) | 494 |
| Eco105I | TACGTA | 1 cut(s) | 849 |
| Eco130I | CCWWGG | 2 cut(s) | 530, 1474 |
| Eco32I | GATATC | 1 cut(s) | 659 |
| Eco47I | GGWCC | 1 cut(s) | 371 |
| Eco88I | CYCGRG | 1 cut(s) | 1433 |
| EcoRII | CCWGG | 2 cut(s) | 894, 1159 |
| EcoRV | GATATC | 1 cut(s) | 659 |
| EcoT14I | CCWWGG | 2 cut(s) | 530, 1474 |
| EcoT22I | ATGCAT | 1 cut(s) | 1350 |
| ErhI | CCWWGG | 2 cut(s) | 530, 1474 |
| Esp3I | CGTCTC | 2 cut(s) | 121, 137 |
| FaeI | CATG | 6 cut(s) | 165, 205, 499, 534, 1352, 1478 |
| FaqI | GGGAC | 2 cut(s) | 131, 736 |
| FatI | CATG | 6 cut(s) | 161, 201, 495, 530, 1348, 1474 |
| FbaI | TGATCA | 2 cut(s) | 828, 909 |
| Fnu4HI | GCNGC | 6 cut(s) | 207, 210, 219, 714, 1032, 1452 |
| FokI | GGATG | 2 cut(s) | 715, 875 |
| Fsp4HI | GCNGC | 6 cut(s) | 207, 210, 219, 714, 1032, 1452 |
| FspBI | CTAG | 3 cut(s) | 749, 1028, 1100 |
| GlaI | GCGC | 1 cut(s) | 143 |
| GluI | GCNGC | 6 cut(s) | 207, 210, 219, 714, 1032, 1452 |
| GsuI | CTGGAG | 3 cut(s) | 617, 789, 1182 |
| HapII | CCGG | 2 cut(s) | 854, 1446 |
| HgaI | GACGC | 5 cut(s) | 119, 119, 133, 154, 303 |
| HhaI | GCGC | 1 cut(s) | 144 |
| Hin1I | GRCGYC | 1 cut(s) | 111 |
| Hin1II | CATG | 6 cut(s) | 165, 205, 499, 534, 1352, 1478 |
| Hin6I | GCGC | 1 cut(s) | 142 |
| HinP1I | GCGC | 1 cut(s) | 142 |
| HindIII | AAGCTT | 2 cut(s) | 270, 1132 |
| HinfI | GANTC | 7 cut(s) | 9, 194, 231, 237, 731, 1431, 1470 |
| HpaII | CCGG | 2 cut(s) | 854, 1446 |
| HphI | GGTGA | 4 cut(s) | 490, 620, 895, 1132 |
| Hpy166II | GTNNAC | 4 cut(s) | 244, 619, 1012, 1046 |
| Hpy188I | TCNGA | 6 cut(s) | 193, 236, 309, 319, 445, 736 |
| Hpy8I | GTNNAC | 4 cut(s) | 244, 619, 1012, 1046 |
| Hpy99I | CGWCG | 2 cut(s) | 71, 170 |
| HpyAV | CCTTC | 6 cut(s) | 384, 391, 595, 599, 1087, 1457 |
| HpyCH4III | ACNGT | 6 cut(s) | 187, 616, 682, 1043, 1120, 1184 |
| HpyCH4IV | ACGT | 2 cut(s) | 621, 848 |
| HpyCH4V | TGCA | 9 cut(s) | 212, 244, 689, 963, 1283, 1307, 1348, 1393, 1451 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 127, 215, 218, 320, 1163 |
| HpyF3I | CTNAG | 7 cut(s) | 134, 757, 900, 1035, 1078, 1318, 1427 |
| HpySE526I | ACGT | 2 cut(s) | 621, 848 |
| Hsp92I | GRCGYC | 1 cut(s) | 111 |
| Hsp92II | CATG | 6 cut(s) | 165, 205, 499, 534, 1352, 1478 |
| HspAI | GCGC | 1 cut(s) | 142 |
| Kpn2I | TCCGGA | 1 cut(s) | 853 |
| Ksp22I | TGATCA | 2 cut(s) | 828, 909 |
| Kzo9I | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| LmnI | GCTCC | 5 cut(s) | 770, 1109, 1162, 1163, 1298 |
| Lsp1109I | GCAGC | 6 cut(s) | 193, 196, 205, 700, 1018, 1463 |
| LweI | GCATC | 3 cut(s) | 310, 1077, 1121 |
| MaeI | CTAG | 3 cut(s) | 749, 1028, 1100 |
| MaeII | ACGT | 2 cut(s) | 621, 848 |
| MaeIII | GTNAC | 4 cut(s) | 284, 625, 883, 1082 |
| MalI | GATC | 7 cut(s) | 158, 543, 830, 911, 949, 955, 1374 |
| MbiI | CCGCTC | 1 cut(s) | 1301 |
| MboI | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| MboII | GAAGA | 8 cut(s) | 26, 369, 493, 511, 581, 853, 1207, 1377 |
| MflI | RGATCY | 3 cut(s) | 947, 953, 1372 |
| MhlI | GDGCHC | 3 cut(s) | 246, 345, 756 |
| MluCI | AATT | 1 cut(s) | 419 |
| MluI | ACGCGT | 1 cut(s) | 128 |
| MlyI | GAGTC | 1 cut(s) | 1425 |
| MmeI | TCCRAC | 4 cut(s) | 768, 840, 967, 1276 |
| Mph1103I | ATGCAT | 1 cut(s) | 1350 |
| MroI | TCCGGA | 1 cut(s) | 853 |
| MseI | TTAA | 3 cut(s) | 330, 651, 932 |
| MspA1I | CMGCKG | 1 cut(s) | 29 |
| MspI | CCGG | 2 cut(s) | 854, 1446 |
| MspR9I | CCNGG | 2 cut(s) | 896, 1161 |
| MvaI | CCWGG | 2 cut(s) | 896, 1161 |
| MvnI | CGCG | 3 cut(s) | 130, 144, 1268 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 127, 215, 218, 320, 1163 |
| NcoI | CCATGG | 2 cut(s) | 530, 1474 |
| NdeII | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| NlaIII | CATG | 6 cut(s) | 165, 205, 499, 534, 1352, 1478 |
| NlaIV | GGNNCC | 2 cut(s) | 1158, 1165 |
| NmuCI | GTSAC | 3 cut(s) | 284, 883, 1082 |
| NsiI | ATGCAT | 1 cut(s) | 1350 |
| NspI | RCATGY | 1 cut(s) | 1352 |
| NspV | TTCGAA | 1 cut(s) | 12 |
| PaeI | GCATGC | 1 cut(s) | 1352 |
| PaeR7I | CTCGAG | 1 cut(s) | 1433 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 449 |
| PfeI | GAWTC | 6 cut(s) | 9, 194, 231, 237, 731, 1470 |
| PflFI | GACNNNGTC | 2 cut(s) | 113, 185 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1480 |
| PfoI | TCCNGGA | 1 cut(s) | 1159 |
| PinAI | ACCGGT | 1 cut(s) | 1445 |
| PkrI | GCNGC | 6 cut(s) | 208, 211, 220, 715, 1033, 1453 |
| PleI | GAGTC | 1 cut(s) | 1425 |
| PpsI | GAGTC | 1 cut(s) | 1425 |
| Ppu21I | YACGTR | 1 cut(s) | 849 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1483 |
| Psp6I | CCWGG | 2 cut(s) | 894, 1159 |
| PspGI | CCWGG | 2 cut(s) | 894, 1159 |
| PspN4I | GGNNCC | 2 cut(s) | 1158, 1165 |
| PspPI | GGNCC | 1 cut(s) | 371 |
| PspXI | VCTCGAGB | 1 cut(s) | 1433 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 503, 535, 797, 829 |
| PstNI | CAGNNNCTG | 1 cut(s) | 680 |
| PsuI | RGATCY | 3 cut(s) | 947, 953, 1372 |
| PsyI | GACNNNGTC | 2 cut(s) | 113, 185 |
| PvuII | CAGCTG | 1 cut(s) | 29 |
| RsaI | GTAC | 4 cut(s) | 893, 1013, 1047, 1444 |
| RsaNI | GTAC | 4 cut(s) | 892, 1012, 1046, 1443 |
| SaqAI | TTAA | 3 cut(s) | 330, 651, 932 |
| SatI | GCNGC | 6 cut(s) | 207, 210, 219, 714, 1032, 1452 |
| Sau3AI | GATC | 7 cut(s) | 156, 541, 828, 909, 947, 953, 1372 |
| Sau96I | GGNCC | 1 cut(s) | 371 |
| SchI | GAGTC | 1 cut(s) | 1425 |
| ScrFI | CCNGG | 2 cut(s) | 896, 1161 |
| SduI | GDGCHC | 3 cut(s) | 246, 345, 756 |
| SfaNI | GCATC | 3 cut(s) | 310, 1077, 1121 |
| SfcI | CTRYAG | 1 cut(s) | 717 |
| Sfr274I | CTCGAG | 1 cut(s) | 1433 |
| SfuI | TTCGAA | 1 cut(s) | 12 |
| SinI | GGWCC | 1 cut(s) | 371 |
| SlaI | CTCGAG | 1 cut(s) | 1433 |
| SmlI | CTYRAG | 4 cut(s) | 267, 821, 1274, 1433 |
| SmoI | CTYRAG | 4 cut(s) | 267, 821, 1274, 1433 |
| SnaBI | TACGTA | 1 cut(s) | 849 |
| SphI | GCATGC | 1 cut(s) | 1352 |
| Sse9I | AATT | 1 cut(s) | 419 |
| SsiI | CCGC | 3 cut(s) | 560, 1266, 1301 |
| SspMI | CTAG | 3 cut(s) | 749, 1028, 1100 |
| StyD4I | CCNGG | 2 cut(s) | 894, 1159 |
| StyI | CCWWGG | 2 cut(s) | 530, 1474 |
| TaaI | ACNGT | 6 cut(s) | 187, 616, 682, 1043, 1120, 1184 |
| TaiI | ACGT | 2 cut(s) | 624, 851 |
| TaqI | TCGA | 9 cut(s) | 12, 96, 155, 261, 452, 809, 1022, 1177, 1434 |
| TasI | AATT | 1 cut(s) | 419 |
| TatI | WGTACW | 1 cut(s) | 1011 |
| TfiI | GAWTC | 6 cut(s) | 9, 194, 231, 237, 731, 1470 |
| Tru1I | TTAA | 3 cut(s) | 330, 651, 932 |
| Tru9I | TTAA | 3 cut(s) | 330, 651, 932 |
| TscAI | CASTG | 3 cut(s) | 112, 190, 1326 |
| TseFI | GTSAC | 3 cut(s) | 284, 883, 1082 |
| TseI | GCWGC | 6 cut(s) | 206, 209, 218, 713, 1031, 1451 |
| Tsp45I | GTSAC | 3 cut(s) | 284, 883, 1082 |
| TspDTI | ATGAA | 5 cut(s) | 82, 473, 512, 1185, 1206 |
| TspGWI | ACGGA | 2 cut(s) | 265, 272 |
| TspRI | CASTG | 3 cut(s) | 112, 190, 1326 |
| Tth111I | GACNNNGTC | 2 cut(s) | 113, 185 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1480 |
| VneI | GTGCAC | 1 cut(s) | 242 |
| VpaK11BI | GGWCC | 1 cut(s) | 371 |
| XceI | RCATGY | 1 cut(s) | 1352 |
| XhoI | CTCGAG | 1 cut(s) | 1433 |
| XspI | CTAG | 3 cut(s) | 749, 1028, 1100 |
| Zsp2I | ATGCAT | 1 cut(s) | 1350 |