Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000729

Physical Location & Seq

Genomic Coordinates Forward (+)

36848329 .. 36851936

Gene Structure 3608 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1237500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGTCCGCCTCAATCTTCGAAGACGGCTTCTCCTCCGAGCGCCTCTTCAACCAGGGCTACTCCTACACCTACGATGACGTCATCTTCCTCCCTCACTATATCGACTTCCCCACCGACTCCGTCCATCCAGCCTCCAACCTCAGCCGCCGCGTCCCTCTCTCGATCCCCTGCGTCTCGTCCCCGATGGACACCGTCACGGAGTCCCACATGGCCATCTCCATGGCCGCACTCGGCGGCATTGGCATCATCCACTCCAACTCGACGTCGTCCGAGCAAGCGCACATGGTCCGGTCCGTCAAGTCCCGCCGGGTCCCGCTTCTTTCCAATCCCGTATTCAAATCCCCAAGCGATCGAATCCAATCCGACGACGTTTTCGACGCTGCCAATCCCTACATCCTGGTTACCGAGAGTGGTGGCCCCAGTTCGAAGCTTTTGGGGTACGTGACTGGTAAGGATTGGGTGAAGTTGGGGGACAAGGAGGTGAAGATATACGATTACATGGTGAACTGTACGGACTTCACGGTGCCGTGGAGTTACGATTTGGGGGCGATTGGGGAGTACATGGAGCAGAAAAAGCGAGATGTGGTGGCGACTGTGAAGGACAATGAGGTTGTGGATGTGGTGGCGAAGGAGGAGGTGGAGAGGATTAAGGGGTACCCCAAATTGGGGGTTGGGACGGTGGGGCCCGACGGGACATGGCGTGTCGGGGCGGCGATCGGGACTAAGGAGTCGGATAAGGAGAGATTGGAGGAGTTGGTGAAGGCAGGGATTGATGTAGTGGTGTTGGATAGCTCTCAGGGGAACTCCATTTATCAGATTGAGATGATAAAGTATGTGAAGAAAATGTACTCGAATTTGGATGTAGTTGGCGGGAATGTGGTGACGGTGAGTCAGGCGCAGAATTTGATTCAGGCTGGTGTTGATGGGTTGAGGGTTGGAATGGGATCTGGCTCAATCTGTACCACTCAAGAGGTCTGCGCCGTCGGACGCGGACAGGCAACTGCTGTTTACAAGGTTGCATCTATCGCTTCACAAAGTGGTGTGCCTGTGATTGCTGATGGTGGAATTTCCAATTCTGGACACATTGTGAAGGCCTTGGTCCTAGGGGCTTCTACCGTCATGATGGGAAGCTTCTTAGCTGGGAGCACTGAGGCTCCCGGGGCTTTTGTGTATCAGAATGGTCGCCGAGTCAAAAAATATCGAGGCATGGGATCTCTTGAAGCAATGACAAAAGGGAGTGATCAGAGGTACTTGGGGGATACAGCTAAACTGAAAATTGCACAGGGGGTGGTTGGGGCAGTTGCAGACAAAGGTTCTGTTTTGAAGTTCATACCATACACAATGCAAGCTGTAAAACAAGGCTTCCAAGATCTTGGTGCTTCCTCACTGGTGTCTGCTCATGACCTCTTAAAATCAAGCATTTTAAGGCTAGAGGTAAGAACAGGAGCAGCACAAGTAGAAGGTGGAGTTCATGGTCTGGTTTCGTACGAGAAGAAATCGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006163 GO:0006164 GO:0006183 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0016491 GO:0016614 GO:0016616 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046039 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0050896 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00230 ko00983 ko01100 ko01110 map00230 map00983 map01100 map01110

Pfam Domains

Protein Families

IMPDH

Protein Analysis

502

Amino Acids

53.57

Weight (kDa)

6.48

Isoelectric Point (pI)

31.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IMPDH	PF00478	22 - 491	2.3e-123	IMP dehydrogenase / GMP reductase domain
FMN_dh	PF01070	269 - 378	3.1e-07	FMN-dependent dehydrogenase
NMO	PF03060	289 - 386	6.7e-07	Nitronate monooxygenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000729)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16350 AT1G79470
fragaria_vesca	FvH4_3g14430 FvH4_3g14430
malus_domestica	MD05G1237000.v1.1 MD05G1237500.v1.1 MD10G1213800.v1.1
prunus_persica	Prupe.4G129200_v2.0.a1 Prupe.4G129200_v2.0.a1
pyrus_communis	pycom05g21380 pycom10g18050 pycom10g18080 pycom10g18140 pycom10g18190
rosa_chinensis	RchiOBHm_Chr1g0350061 RchiOBHm_Chr2g0101951 RchiOBHm_Chr2g0155871 RchiOBHm_Chr3g0468611 RchiOBHm_Chr3g0493461 RchiOBHm_Chr4g0401281 RchiOBHm_Chr4g0402021 RchiOBHm_Chr5g0023561 RchiOBHm_Chr5g0023571 RchiOBHm_Chr5g0023741 RchiOBHm_Chr7g0231801 RchiOBHm_Chr7g0234111 RchiOBHm_Chr7g0238791 RchiOBHm_Chr7g0239601
rosa_laevigata	RLG00000032733
rosa_multiflora	Rmu_co8005014.1_g000001 Rmu_co8223904.1_g000001 Rmu_sc0000087.1_g000007 Rmu_sc0000110.1_g000028 Rmu_sc0000172.1_g000003 Rmu_sc0000689.1_g000019 Rmu_sc0000870.1_g000014 Rmu_sc0001372.1_g000011 Rmu_sc0001516.1_g000086 Rmu_sc0001653.1_g000008 Rmu_sc0002862.1_g000001 Rmu_sc0002862.1_g000002 Rmu_sc0008652.1_g000002 Rmu_sc0011789.1_g000002 Rmu_sc0013252.1_g000001 Rmu_sc0017256.1_g000001 Rmu_sc0017907.1_g000002 Rmu_sc0017907.1_g000003 Rmu_sc0018737.1_g000003 Rmu_sc0020536.1_g000001 Rmu_sc0025127.1_g000001 Rmu_sc0028608.1_g000001 Rmu_sc0029703.1_g000001 Rmu_ssc0000027.1_g000015
rosa_roxburghii	Rroxscaffold_1G00055450
rosa_rugosa	Rorug03G0326300 Rorug05G0079400 Rorug05G0352300
rosa_samantha	Rh5AG167200 Rh5AG169300 Rh5BG166700 Rh5CG181100 Rh5CG182300 Rh5DG168600 Rh6BG409700
rosa_wichuraiana	Rw0G001580 Rw0G004990 Rw1G017410 Rw2G027840 Rw4G002740 Rw5G015190 Rw5G015310 Rw7G033830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	727
AatII	GACGTC	2 cut(s)	81, 266
Acc65I	GGTACC	1 cut(s)	654
AccB1I	GGYRCC	2 cut(s)	523, 654
AccII	CGCG	2 cut(s)	150, 990
AclWI	GGATC	3 cut(s)	157, 952, 1219
AcoI	YGGCCR	2 cut(s)	210, 222
AcsI	RAATTY	3 cut(s)	853, 901, 1065
AcyI	GRCGYC	2 cut(s)	78, 263
AdeI	CACNNNGTG	2 cut(s)	1037, 1087
AfaI	GTAC	8 cut(s)	440, 511, 560, 656, 848, 961, 1250, 1487
AfiI	CCNNNNNNNGG	3 cut(s)	664, 665, 666
AgsI	TTSAA	4 cut(s)	49, 337, 1220, 1324
AhdI	GACNNNNNGTC	1 cut(s)	888
AjnI	CCWGG	2 cut(s)	51, 396
AjuI	GAANNNNNNNTTGG	2 cut(s)	317, 349
AluBI	AGCT	6 cut(s)	430, 792, 1131, 1139, 1265, 1349
AluI	AGCT	6 cut(s)	430, 792, 1131, 1139, 1265, 1349
Alw21I	GWGCWC	1 cut(s)	1148
Alw26I	GTCTC	1 cut(s)	178
AlwI	GGATC	3 cut(s)	157, 952, 1219
Ama87I	CYCGRG	1 cut(s)	1157
AoxI	GGCC	5 cut(s)	210, 222, 415, 683, 1092
ApaI	GGGCCC	1 cut(s)	687
ApeKI	GCWGC	2 cut(s)	380, 1448
ApoI	RAATTY	3 cut(s)	853, 901, 1065
ArsI	GACNNNNNNTTYG	2 cut(s)	356, 388
Asp718I	GGTACC	1 cut(s)	654
AspA2I	CCTAGG	1 cut(s)	1102
AspLEI	GCGC	4 cut(s)	42, 280, 898, 980
AspS9I	GGNCC	7 cut(s)	286, 291, 310, 416, 683, 684, 1099
AsuC2I	CCSGG	3 cut(s)	308, 1158, 1159
AsuHPI	GGTGA	6 cut(s)	472, 493, 514, 769, 892, 898
AsuII	TTCGAA	2 cut(s)	18, 425
AvaI	CYCGRG	1 cut(s)	1157
AvaII	GGWCC	4 cut(s)	286, 291, 310, 1099
AvrII	CCTAGG	1 cut(s)	1102
BaeGI	GKGCMC	1 cut(s)	687
BalI	TGGCCA	1 cut(s)	212
BanI	GGYRCC	2 cut(s)	523, 654
BanII	GRGCYC	1 cut(s)	687
BarI	GAAGNNNNNNTAC	2 cut(s)	830, 862
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	1 cut(s)	1148
BbvCI	CCTCAGC	1 cut(s)	140
BbvI	GCAGC	2 cut(s)	367, 1460
BccI	CCATC	6 cut(s)	132, 178, 221, 917, 1052, 1117
BceAI	ACGGC	3 cut(s)	40, 511, 965
BciT130I	CCWGG	2 cut(s)	53, 398
BciVI	GTATCC	1 cut(s)	1252
BclI	TGATCA	1 cut(s)	1240
BcnI	CCSGG	3 cut(s)	308, 1158, 1159
BcoDI	GTCTC	1 cut(s)	178
BfaI	CTAG	2 cut(s)	1103, 1430
BfoI	RGCGCY	1 cut(s)	43
BfuI	GTATCC	1 cut(s)	1252
BglII	AGATCT	1 cut(s)	1369
BisI	GCNGC	7 cut(s)	145, 148, 225, 235, 381, 711, 1449
BlnI	CCTAGG	1 cut(s)	1102
BlsI	GCNGC	7 cut(s)	146, 149, 226, 236, 382, 712, 1450
Bme1390I	CCNGG	5 cut(s)	53, 308, 398, 1158, 1159
Bme18I	GGWCC	4 cut(s)	286, 291, 310, 1099
BmeRI	GACNNNNNGTC	1 cut(s)	888
BmeT110I	CYCGRG	1 cut(s)	1157
BmgT120I	GGNCC	7 cut(s)	286, 291, 310, 416, 683, 684, 1099
BmiI	GGNNCC	8 cut(s)	311, 312, 418, 525, 656, 684, 685, 1155
BmrFI	CCNGG	5 cut(s)	53, 308, 398, 1158, 1159
BmrI	ACTGGG	1 cut(s)	414
BmsI	GCATC	2 cut(s)	252, 1028
BmuI	ACTGGG	1 cut(s)	414
BpiI	GAAGAC	1 cut(s)	27
Bpu10I	CCTNAGC	1 cut(s)	140
Bpu14I	TTCGAA	2 cut(s)	18, 425
BpuEI	CTTGAG	1 cut(s)	951
BpuMI	CCSGG	3 cut(s)	308, 1158, 1159
BsaAI	YACGTR	1 cut(s)	442
BsaHI	GRCGYC	2 cut(s)	78, 263
BsaJI	CCNNGG	7 cut(s)	52, 219, 527, 1095, 1102, 1157, 1158
BsaWI	WCCGGW	1 cut(s)	288
BsaXI	ACNNNNNCTCC	4 cut(s)	14, 44, 1138, 1168
Bsc4I	CCNNNNNNNGG	3 cut(s)	664, 665, 666
Bse1I	ACTGG	3 cut(s)	420, 451, 1392
Bse3DI	GCAATG	1 cut(s)	1230
BseBI	CCWGG	2 cut(s)	53, 398
BseDI	CCNNGG	7 cut(s)	52, 219, 527, 1095, 1102, 1157, 1158
BseGI	GGATG	5 cut(s)	124, 246, 393, 622, 865
BseLI	CCNNNNNNNGG	3 cut(s)	664, 665, 666
BseMI	GCAATG	1 cut(s)	1230
BseMII	CTCAG	3 cut(s)	154, 809, 1140
BseNI	ACTGG	3 cut(s)	420, 451, 1392
BseRI	GAGGAG	3 cut(s)	22, 647, 764
BseSI	GKGCMC	1 cut(s)	687
BseXI	GCAGC	2 cut(s)	367, 1460
BseYI	CCCAGC	1 cut(s)	1139
Bsh1236I	CGCG	2 cut(s)	150, 990
Bsh1285I	CGRYCG	2 cut(s)	352, 717
BshFI	GGCC	5 cut(s)	212, 224, 417, 685, 1094
BshNI	GGYRCC	2 cut(s)	523, 654
BsiEI	CGRYCG	2 cut(s)	352, 717
BsiHKAI	GWGCWC	1 cut(s)	1148
BsiHKCI	CYCGRG	1 cut(s)	1157
BsiSI	CCGG	3 cut(s)	289, 307, 1158
BsiWI	CGTACG	1 cut(s)	1485
BslFI	GGGAC	9 cut(s)	137, 163, 187, 286, 296, 485, 688, 706, 733
BslI	CCNNNNNNNGG	3 cut(s)	664, 665, 666
BsmAI	GTCTC	1 cut(s)	178
BsmBI	CGTCTC	1 cut(s)	178
BsmFI	GGGAC	9 cut(s)	137, 163, 187, 286, 296, 485, 688, 706, 733
BsnI	GGCC	5 cut(s)	212, 224, 417, 685, 1094
BsoBI	CYCGRG	1 cut(s)	1157
Bsp119I	TTCGAA	2 cut(s)	18, 425
Bsp120I	GGGCCC	1 cut(s)	683
Bsp1286I	GDGCHC	2 cut(s)	687, 1148
Bsp143I	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
Bsp19I	CCATGG	1 cut(s)	219
BspANI	GGCC	5 cut(s)	212, 224, 417, 685, 1094
BspCNI	CTCAG	3 cut(s)	153, 808, 1141
BspFNI	CGCG	2 cut(s)	150, 990
BspHI	TCATGA	2 cut(s)	1119, 1399
BspLI	GGNNCC	8 cut(s)	311, 312, 418, 525, 656, 684, 685, 1155
BspPI	GGATC	3 cut(s)	157, 952, 1219
BspT104I	TTCGAA	2 cut(s)	18, 425
BspT107I	GGYRCC	2 cut(s)	523, 654
BsrDI	GCAATG	1 cut(s)	1230
BsrI	ACTGG	3 cut(s)	420, 451, 1392
BssECI	CCNNGG	7 cut(s)	52, 219, 527, 1095, 1102, 1157, 1158
BssMI	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
BssNI	GRCGYC	2 cut(s)	78, 263
BssT1I	CCWWGG	3 cut(s)	219, 1095, 1102
Bst2UI	CCWGG	2 cut(s)	53, 398
Bst4CI	ACNGT	7 cut(s)	193, 509, 523, 595, 679, 886, 1117
Bst6I	CTCTTC	1 cut(s)	50
BstACI	GRCGYC	2 cut(s)	78, 263
BstBAI	YACGTR	1 cut(s)	442
BstBI	TTCGAA	2 cut(s)	18, 425
BstC8I	GCNNGC	2 cut(s)	276, 1347
BstDEI	CTNAG	5 cut(s)	140, 723, 795, 1135, 1149
BstDSI	CCRYGG	2 cut(s)	219, 527
BstEII	GGTNACC	1 cut(s)	400
BstF5I	GGATG	5 cut(s)	124, 246, 393, 622, 865
BstFNI	CGCG	2 cut(s)	150, 990
BstH2I	RGCGCY	1 cut(s)	43
BstHHI	GCGC	4 cut(s)	42, 280, 898, 980
BstKTI	GATC	7 cut(s)	165, 352, 717, 947, 1214, 1243, 1372
BstMAI	GTCTC	1 cut(s)	178
BstMBI	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
BstMCI	CGRYCG	2 cut(s)	352, 717
BstMWI	GCNNNNNNNGC	4 cut(s)	240, 574, 1025, 1160
BstNI	CCWGG	2 cut(s)	53, 398
BstPI	GGTNACC	1 cut(s)	400
BstSCI	CCNGG	5 cut(s)	51, 306, 396, 1156, 1157
BstSLI	GKGCMC	1 cut(s)	687
BstUI	CGCG	2 cut(s)	150, 990
BstV1I	GCAGC	2 cut(s)	367, 1460
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	3 cut(s)	944, 1211, 1369
BstXI	CCANNNNNNTGG	2 cut(s)	220, 1373
BstYI	RGATCY	3 cut(s)	944, 1211, 1369
BsuI	GTATCC	1 cut(s)	1252
BsuRI	GGCC	5 cut(s)	212, 224, 417, 685, 1094
BtgI	CCRYGG	2 cut(s)	219, 527
BtsCI	GGATG	5 cut(s)	124, 246, 393, 622, 865
BtsIMutI	CAGTG	2 cut(s)	1146, 1385
Cac8I	GCNNGC	2 cut(s)	276, 1347
CciI	TCATGA	2 cut(s)	1119, 1399
CfoI	GCGC	4 cut(s)	42, 280, 898, 980
Cfr13I	GGNCC	7 cut(s)	286, 291, 310, 416, 683, 684, 1099
Cfr9I	CCCGGG	1 cut(s)	1157
CpoI	CGGWCCG	1 cut(s)	291
CseI	GACGC	4 cut(s)	139, 160, 386, 996
Csp6I	GTAC	8 cut(s)	439, 510, 559, 655, 847, 960, 1249, 1486
CspI	CGGWCCG	1 cut(s)	291
CviQI	GTAC	8 cut(s)	439, 510, 559, 655, 847, 960, 1249, 1486
DdeI	CTNAG	5 cut(s)	140, 723, 795, 1135, 1149
DpnI	GATC	7 cut(s)	164, 351, 716, 946, 1213, 1242, 1371
DpnII	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
DraIII	CACNNNGTG	2 cut(s)	1037, 1087
DrdI	GACNNNNNNGTC	1 cut(s)	727
DriI	GACNNNNNGTC	1 cut(s)	888
DseDI	GACNNNNNNGTC	1 cut(s)	727
EaeI	YGGCCR	2 cut(s)	210, 222
Eam1104I	CTCTTC	1 cut(s)	50
Eam1105I	GACNNNNNGTC	1 cut(s)	888
EarI	CTCTTC	1 cut(s)	50
Eco130I	CCWWGG	3 cut(s)	219, 1095, 1102
Eco147I	AGGCCT	1 cut(s)	1094
Eco24I	GRGCYC	1 cut(s)	687
Eco47I	GGWCC	4 cut(s)	286, 291, 310, 1099
Eco88I	CYCGRG	1 cut(s)	1157
Eco91I	GGTNACC	1 cut(s)	400
EcoO109I	RGGNCCY	2 cut(s)	310, 683
EcoO65I	GGTNACC	1 cut(s)	400
EcoRII	CCWGG	2 cut(s)	51, 396
EcoT14I	CCWWGG	3 cut(s)	219, 1095, 1102
EcoT38I	GRGCYC	1 cut(s)	687
ErhI	CCWWGG	3 cut(s)	219, 1095, 1102
Esp3I	CGTCTC	1 cut(s)	178
FaqI	GGGAC	9 cut(s)	137, 163, 187, 286, 296, 485, 688, 706, 733
FauI	CCCGC	3 cut(s)	311, 321, 863
FbaI	TGATCA	1 cut(s)	1240
Fnu4HI	GCNGC	7 cut(s)	145, 148, 225, 235, 381, 711, 1449
FokI	GGATG	5 cut(s)	111, 233, 380, 629, 872
FriOI	GRGCYC	1 cut(s)	687
Fsp4HI	GCNGC	7 cut(s)	145, 148, 225, 235, 381, 711, 1449
FspBI	CTAG	2 cut(s)	1103, 1430
GlaI	GCGC	4 cut(s)	41, 279, 897, 979
GluI	GCNGC	7 cut(s)	145, 148, 225, 235, 381, 711, 1449
GsaI	CCCAGC	1 cut(s)	1143
HaeII	RGCGCY	1 cut(s)	43
HaeIII	GGCC	5 cut(s)	212, 224, 417, 685, 1094
HapII	CCGG	3 cut(s)	289, 307, 1158
HgaI	GACGC	4 cut(s)	139, 160, 386, 996
HhaI	GCGC	4 cut(s)	42, 280, 898, 980
Hin1I	GRCGYC	2 cut(s)	78, 263
Hin6I	GCGC	4 cut(s)	40, 278, 896, 978
HinP1I	GCGC	4 cut(s)	40, 278, 896, 978
HindIII	AAGCTT	2 cut(s)	428, 1129
HinfI	GANTC	7 cut(s)	116, 200, 354, 728, 889, 907, 1188
HpaII	CCGG	3 cut(s)	289, 307, 1158
HphI	GGTGA	6 cut(s)	472, 493, 514, 769, 892, 898
Hpy166II	GTNNAC	2 cut(s)	505, 1009
Hpy188I	TCNGA	8 cut(s)	37, 271, 364, 733, 816, 986, 1176, 1245
Hpy188III	TCNNGA	7 cut(s)	160, 718, 968, 1077, 1120, 1217, 1400
Hpy8I	GTNNAC	2 cut(s)	505, 1009
Hpy99I	CGWCG	7 cut(s)	265, 268, 368, 371, 380, 692, 986
HpyAV	CCTTC	5 cut(s)	592, 622, 754, 1084, 1454
HpyCH4III	ACNGT	7 cut(s)	193, 509, 523, 595, 679, 886, 1117
HpyCH4IV	ACGT	4 cut(s)	78, 263, 369, 441
HpyCH4V	TGCA	4 cut(s)	1019, 1280, 1304, 1345
HpyF10VI	GCNNNNNNNGC	4 cut(s)	240, 574, 1025, 1160
HpyF3I	CTNAG	5 cut(s)	140, 723, 795, 1135, 1149
HpySE526I	ACGT	4 cut(s)	78, 263, 369, 441
Hsp92I	GRCGYC	2 cut(s)	78, 263
HspAI	GCGC	4 cut(s)	40, 278, 896, 978
KflI	GGGWCCC	1 cut(s)	310
KpnI	GGTACC	1 cut(s)	658
Ksp22I	TGATCA	1 cut(s)	1240
Kzo9I	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
LmnI	GCTCC	4 cut(s)	565, 1143, 1159, 1445
Lsp1109I	GCAGC	2 cut(s)	367, 1460
LweI	GCATC	2 cut(s)	252, 1028
MaeI	CTAG	2 cut(s)	1103, 1430
MaeII	ACGT	4 cut(s)	78, 263, 369, 441
MaeIII	GTNAC	5 cut(s)	193, 400, 442, 533, 880
MalI	GATC	7 cut(s)	164, 351, 716, 946, 1213, 1242, 1371
MboI	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
MboII	GAAGA	7 cut(s)	7, 32, 37, 76, 496, 850, 1504
MflI	RGATCY	3 cut(s)	944, 1211, 1369
MhlI	GDGCHC	2 cut(s)	687, 1148
MlsI	TGGCCA	1 cut(s)	212
MluCI	AATT	6 cut(s)	662, 853, 901, 1065, 1072, 1275
MluNI	TGGCCA	1 cut(s)	212
MlyI	GAGTC	5 cut(s)	110, 209, 737, 898, 1197
MmeI	TCCRAC	7 cut(s)	159, 279, 387, 711, 765, 916, 964
Mox20I	TGGCCA	1 cut(s)	212
MscI	TGGCCA	1 cut(s)	212
MseI	TTAA	3 cut(s)	648, 1409, 1424
MslI	CAYNNNNRTG	1 cut(s)	218
Msp20I	TGGCCA	1 cut(s)	212
MspI	CCGG	3 cut(s)	289, 307, 1158
MspR9I	CCNGG	5 cut(s)	53, 308, 398, 1158, 1159
MvaI	CCWGG	2 cut(s)	53, 398
MvnI	CGCG	2 cut(s)	150, 990
MwoI	GCNNNNNNNGC	4 cut(s)	240, 574, 1025, 1160
NciI	CCSGG	3 cut(s)	308, 1158, 1159
NcoI	CCATGG	1 cut(s)	219
NdeII	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
NlaIV	GGNNCC	8 cut(s)	311, 312, 418, 525, 656, 684, 685, 1155
NmeAIII	GCCGAG	2 cut(s)	210, 1211
NmuCI	GTSAC	3 cut(s)	193, 442, 880
NspV	TTCGAA	2 cut(s)	18, 425
PagI	TCATGA	2 cut(s)	1119, 1399
PceI	AGGCCT	1 cut(s)	1094
PcsI	WCGNNNNNNNCGW	1 cut(s)	372
PfeI	GAWTC	2 cut(s)	354, 907
Pfl23II	CGTACG	1 cut(s)	1485
PflFI	GACNNNGTC	3 cut(s)	119, 191, 265
PkrI	GCNGC	7 cut(s)	146, 149, 226, 236, 382, 712, 1450
Ple19I	CGATCG	2 cut(s)	352, 717
PleI	GAGTC	5 cut(s)	110, 208, 736, 897, 1196
PpsI	GAGTC	5 cut(s)	110, 208, 736, 897, 1196
Ppu21I	YACGTR	1 cut(s)	442
PpuMI	RGGWCCY	1 cut(s)	310
Psp5II	RGGWCCY	1 cut(s)	310
Psp6I	CCWGG	2 cut(s)	51, 396
PspEI	GGTNACC	1 cut(s)	400
PspFI	CCCAGC	1 cut(s)	1139
PspGI	CCWGG	2 cut(s)	51, 396
PspLI	CGTACG	1 cut(s)	1485
PspN4I	GGNNCC	8 cut(s)	311, 312, 418, 525, 656, 684, 685, 1155
PspOMI	GGGCCC	1 cut(s)	683
PspPI	GGNCC	7 cut(s)	286, 291, 310, 416, 683, 684, 1099
PspPPI	RGGWCCY	1 cut(s)	310
PsuI	RGATCY	3 cut(s)	944, 1211, 1369
PsyI	GACNNNGTC	3 cut(s)	119, 191, 265
PvuI	CGATCG	2 cut(s)	352, 717
RsaI	GTAC	8 cut(s)	440, 511, 560, 656, 848, 961, 1250, 1487
RsaNI	GTAC	8 cut(s)	439, 510, 559, 655, 847, 960, 1249, 1486
RseI	CAYNNNNRTG	1 cut(s)	218
Rsr2I	CGGWCCG	1 cut(s)	291
RsrII	CGGWCCG	1 cut(s)	291
SaqAI	TTAA	3 cut(s)	648, 1409, 1424
SatI	GCNGC	7 cut(s)	145, 148, 225, 235, 381, 711, 1449
Sau3AI	GATC	7 cut(s)	162, 349, 714, 944, 1211, 1240, 1369
Sau96I	GGNCC	7 cut(s)	286, 291, 310, 416, 683, 684, 1099
SchI	GAGTC	5 cut(s)	110, 209, 737, 898, 1197
ScrFI	CCNGG	5 cut(s)	53, 308, 398, 1158, 1159
SduI	GDGCHC	2 cut(s)	687, 1148
SfaNI	GCATC	2 cut(s)	252, 1028
SfuI	TTCGAA	2 cut(s)	18, 425
SinI	GGWCC	4 cut(s)	286, 291, 310, 1099
SmaI	CCCGGG	1 cut(s)	1159
SmiMI	CAYNNNNRTG	1 cut(s)	218
SmlI	CTYRAG	1 cut(s)	966
SmoI	CTYRAG	1 cut(s)	966
Sse9I	AATT	6 cut(s)	662, 853, 901, 1065, 1072, 1275
SseBI	AGGCCT	1 cut(s)	1094
SspMI	CTAG	2 cut(s)	1103, 1430
StuI	AGGCCT	1 cut(s)	1094
StyD4I	CCNGG	5 cut(s)	51, 306, 396, 1156, 1157
StyI	CCWWGG	3 cut(s)	219, 1095, 1102
TaaI	ACNGT	7 cut(s)	193, 509, 523, 595, 679, 886, 1117
TaiI	ACGT	4 cut(s)	81, 266, 372, 444
TaqI	TCGA	9 cut(s)	18, 102, 161, 260, 352, 375, 425, 851, 1201
TasI	AATT	6 cut(s)	662, 853, 901, 1065, 1072, 1275
TatI	WGTACW	2 cut(s)	558, 846
TauI	GCSGC	5 cut(s)	147, 150, 227, 237, 713
TfiI	GAWTC	2 cut(s)	354, 907
Tru1I	TTAA	3 cut(s)	648, 1409, 1424
Tru9I	TTAA	3 cut(s)	648, 1409, 1424
TscAI	CASTG	2 cut(s)	1153, 1392
TseFI	GTSAC	3 cut(s)	193, 442, 880
TseI	GCWGC	2 cut(s)	380, 1448
Tsp45I	GTSAC	3 cut(s)	193, 442, 880
TspDTI	ATGAA	2 cut(s)	1318, 1460
TspGWI	ACGGA	4 cut(s)	109, 212, 283, 527
TspMI	CCCGGG	1 cut(s)	1157
TspRI	CASTG	2 cut(s)	1153, 1392
Tth111I	GACNNNGTC	3 cut(s)	119, 191, 265
VpaK11BI	GGWCC	4 cut(s)	286, 291, 310, 1099
XapI	RAATTY	3 cut(s)	853, 901, 1065
XmaI	CCCGGG	1 cut(s)	1157
XmaJI	CCTAGG	1 cut(s)	1102
XspI	CTAG	2 cut(s)	1103, 1430
ZraI	GACGTC	2 cut(s)	79, 264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.