AT1G64880

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001359
Physical Location & Seq
Forward (+)
24105611 .. 24109243
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64880.1

Sequence Viewer

Length: 1548 bp
ATGTCTTTGAGAACTCGAGCATCGTCATGGCCTCGTCTTCTACTTCGCCGGAACCCTAGATCTTCATTTTCTCGTCAGATATCTACTCTCGATTCCTCAACATCAACATTTCCCAGAAGAATCTCCGGTTACTACTCCTCCTCCTTCTTTCCCGTAAATCAAACGCCATGGATCTCATCTCAGAGGTACCGTTTCTTCTCCTCAAGCACAATGTCACCATCGGAAGCTGATAGAGTAGTGAGAGAACTTATAGCAGAGGTTGAGAAAGAGAAGCAGCGTGAGAGAGAGGAGAGACAAAGACAAGGACTTGATTACAAAGACATTGATGCCGAAGACGAAGAAGATTACTTAGGGATTGAGCCGTTTATCGAGAAGCTTAAGAAGCAGAATTTGAAAGATGATGGTGAATTAAATAGGCGTGAAGAGTCTTCTGATTCGGATAGTGAATATGATGAGATTGATTTGGATGAAGAGAGGAAGAAAGAAGATATCTTTAATAAGAAGTTTCAGAGACATAAGGAGCTTCTTCAGACTCTTACCAAGTCAGAGACACTTGATGAGGCATTTAGATGGATGAACAAATTAGACAAATTTGAGGAGAAGCATTTCAAGCTTAGACCTGAGTATAGGGTTATTGGTGAGCTGATGAATCGTTTGAAGGTAGCTGAAGGGAAAGAGAAGTTTGTTCTGCAGCAGAAATTGAATAGAGCTATGAGGTTGGTTGAGTGGAAAGAGGCTTTTGATCCAAACAACCCTGCTAATTATGGAGTTATTGAACGTGATAATGTTCAAGGTGGTGGTGAAGAAAGAGAAGAGCGTCTTGTTGCTGATGGGCCTAAGGATGACAATGATGATGATGATGATGAAGAGGAGTTTGATGACATGAAAGAAAGGGATGATATTTTGTTGGAGAAGCTGAATGCGATTGACAAGAAACTTGAGATTAAGCTTTCTGAGCTTGATCATACCTTTGGGAAGAAGGGGAAACGGTTGGAAGAGGAGATTAGAGAGCTGGCTGAAGACAGAAATGCTTTGACAGAGAAGAAAAGACAACCTTTATACAGAAAAGGATATGATGTGCATGTGATAGATGTAAAAAAAGTTTGTAAGGTCACAAAGGGTGGCAGGGTTGAAAGGTACACTGCATTGATGGTTTGCGGAAACTATGAAGGTATCATTGGGTATGCAAAAGCAAAAGCTGAGACTGGCCAATCTGCAATGCAGAAGGCATATGAGAAATGCTTTCAGAATCTTCATTACGTAGAGCGGCATGAGGAGCACACCATTGCACACGCAATACAGACTTCTTACAAGAAAACCAAGTTATATCTGTGGCCTGCACCAACCACGACGGGAATGAAAGCTGGGAGAGTTGTCAAAACAATCTTGCTCCTAGCAGGGTTCAAAAATATCAAATCAAAGGTGATTGGTTCAAGGAATTCATACAACACCGTTAAGGCAGTGTTGAAGGCCTTGAATGCGGTCGAAACACCAAAGGATGTTCAGGAGAAGTTTGGCCGGACAGTTGTTGAGAAATATCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

515

Amino Acids

60.24

Weight (kDa)

7.12

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 360 - 422 5.7e-19 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 437 - 504 5.2e-21 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 1267
AciI CCGC 3 cut(s) 1158, 1267, 1481
AclWI GGATC 2 cut(s) 179, 737
AcoI YGGCCR 2 cut(s) 1207, 1516
AcsI RAATTY 3 cut(s) 388, 590, 1438
AcuI CTGAAG 3 cut(s) 512, 687, 1038
AfaI GTAC 2 cut(s) 188, 1139
AflII CTTAAG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 1161, 1193
Alw21I GWGCWC 1 cut(s) 1281
Alw26I GTCTC 4 cut(s) 286, 505, 542, 1196
AlwI GGATC 2 cut(s) 179, 737
Ama87I CYCGRG 1 cut(s) 15
AoxI GGCC 6 cut(s) 29, 833, 1207, 1334, 1470, 1516
ApeKI GCWGC 2 cut(s) 274, 691
ApoI RAATTY 3 cut(s) 388, 590, 1438
Asp700I GAANNNNTTC 1 cut(s) 605
Asp718I GGTACC 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 833
AsuHPI GGTGA 5 cut(s) 207, 416, 650, 812, 1435
AvaI CYCGRG 1 cut(s) 15
AxyI CCTNAGG 1 cut(s) 837
BalI TGGCCA 1 cut(s) 1209
BanI GGYRCC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BbsI GAAGAC 4 cut(s) 29, 339, 420, 1026
Bbv12I GWGCWC 1 cut(s) 1281
BbvI GCAGC 2 cut(s) 286, 703
BccI CCATC 5 cut(s) 226, 395, 564, 824, 1144
BceAI ACGGC 1 cut(s) 346
BclI TGATCA 1 cut(s) 961
BcoDI GTCTC 4 cut(s) 286, 505, 542, 1196
BfaI CTAG 2 cut(s) 57, 1394
BfmI CTRYAG 1 cut(s) 689
BfrI CTTAAG 1 cut(s) 377
BglII AGATCT 1 cut(s) 59
BisI GCNGC 3 cut(s) 275, 692, 1268
BlsI GCNGC 3 cut(s) 276, 693, 1269
BmeT110I CYCGRG 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 833
BmiI GGNNCC 2 cut(s) 53, 188
BmsI GCATC 2 cut(s) 29, 316
BpiI GAAGAC 4 cut(s) 29, 339, 420, 1026
BpuEI CTTGAG 2 cut(s) 187, 959
BsaAI YACGTR 1 cut(s) 1261
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 4 cut(s) 122, 125, 152, 155
Bse1I ACTGG 1 cut(s) 1210
Bse21I CCTNAGG 1 cut(s) 837
Bse3DI GCAATG 2 cut(s) 1224, 1284
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 5 cut(s) 472, 579, 847, 901, 1504
BseMI GCAATG 2 cut(s) 1224, 1284
BseMII CTCAG 4 cut(s) 194, 612, 945, 1191
BseNI ACTGG 1 cut(s) 1210
BseRI GAGGAG 8 cut(s) 127, 130, 190, 302, 611, 884, 1013, 1289
BseXI GCAGC 2 cut(s) 286, 703
BseYI CCCAGC 1 cut(s) 1364
BsgI GTGCAG 1 cut(s) 1323
Bsh1285I CGRYCG 1 cut(s) 1485
BshFI GGCC 6 cut(s) 31, 835, 1209, 1336, 1472, 1518
BshNI GGYRCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 1485
BsiHKAI GWGCWC 1 cut(s) 1281
BsiHKCI CYCGRG 1 cut(s) 15
BsiSI CCGG 3 cut(s) 49, 126, 1519
BsmAI GTCTC 4 cut(s) 286, 505, 542, 1196
BsmI GAATGC 2 cut(s) 925, 1483
BsnI GGCC 6 cut(s) 31, 835, 1209, 1336, 1472, 1518
BsoBI CYCGRG 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 1281
Bsp143I GATC 4 cut(s) 59, 171, 742, 961
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 3 cut(s) 1158, 1267, 1481
BspANI GGCC 6 cut(s) 31, 835, 1209, 1336, 1472, 1518
BspCNI CTCAG 4 cut(s) 193, 613, 946, 1192
BspLI GGNNCC 2 cut(s) 53, 188
BspMAI CTGCAG 1 cut(s) 693
BspPI GGATC 2 cut(s) 179, 737
BspQI GCTCTTC 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 1267
BsrDI GCAATG 2 cut(s) 1224, 1284
BsrI ACTGG 1 cut(s) 1210
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 4 cut(s) 59, 171, 742, 961
BssT1I CCWWGG 1 cut(s) 167
Bst4CI ACNGT 4 cut(s) 191, 990, 1453, 1525
Bst6I CTCTTC 5 cut(s) 417, 465, 807, 861, 990
BstAFI CTTAAG 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 1261
BstC8I GCNNGC 2 cut(s) 1014, 1338
BstDEI CTNAG 7 cut(s) 180, 349, 614, 621, 837, 954, 1200
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 5 cut(s) 472, 579, 847, 901, 1504
BstKTI GATC 4 cut(s) 62, 174, 745, 964
BstMAI GTCTC 4 cut(s) 286, 505, 542, 1196
BstMBI GATC 4 cut(s) 59, 171, 742, 961
BstMCI CGRYCG 1 cut(s) 1485
BstMWI GCNNNNNNNGC 5 cut(s) 382, 610, 955, 1276, 1478
BstNSI RCATGY 1 cut(s) 1085
BstSFI CTRYAG 1 cut(s) 689
BstSNI TACGTA 1 cut(s) 1261
BstV1I GCAGC 2 cut(s) 286, 703
BstV2I GAAGAC 4 cut(s) 29, 339, 420, 1026
BstX2I RGATCY 2 cut(s) 59, 171
BstYI RGATCY 2 cut(s) 59, 171
Bsu36I CCTNAGG 1 cut(s) 837
BsuRI GGCC 6 cut(s) 31, 835, 1209, 1336, 1472, 1518
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 5 cut(s) 472, 579, 847, 901, 1504
BtsI GCAGTG 2 cut(s) 1140, 1467
BtsIMutI CAGTG 2 cut(s) 1140, 1467
Cac8I GCNNGC 2 cut(s) 1014, 1338
Cfr13I GGNCC 1 cut(s) 833
CseI GACGC 1 cut(s) 806
Csp6I GTAC 2 cut(s) 187, 1138
CviAII CATG 5 cut(s) 27, 168, 883, 1082, 1271
CviQI GTAC 2 cut(s) 187, 1138
DdeI CTNAG 7 cut(s) 180, 349, 614, 621, 837, 954, 1200
DpnI GATC 4 cut(s) 61, 173, 744, 963
DpnII GATC 4 cut(s) 59, 171, 742, 961
EaeI YGGCCR 2 cut(s) 1207, 1516
Eam1104I CTCTTC 5 cut(s) 417, 465, 807, 861, 990
EarI CTCTTC 5 cut(s) 417, 465, 807, 861, 990
Eco105I TACGTA 1 cut(s) 1261
Eco130I CCWWGG 1 cut(s) 167
Eco147I AGGCCT 1 cut(s) 1472
Eco32I GATATC 2 cut(s) 81, 490
Eco57I CTGAAG 3 cut(s) 512, 687, 1038
Eco81I CCTNAGG 1 cut(s) 837
Eco88I CYCGRG 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 1438
EcoRV GATATC 2 cut(s) 81, 490
EcoT14I CCWWGG 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 5 cut(s) 30, 171, 886, 1085, 1274
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 1039, 1071
FatI CATG 5 cut(s) 26, 167, 882, 1081, 1270
FauNDI CATATG 1 cut(s) 1231
FbaI TGATCA 1 cut(s) 961
Fnu4HI GCNGC 3 cut(s) 275, 692, 1268
FokI GGATG 5 cut(s) 479, 586, 854, 908, 1511
Fsp4HI GCNGC 3 cut(s) 275, 692, 1268
FspBI CTAG 2 cut(s) 57, 1394
GluI GCNGC 3 cut(s) 275, 692, 1268
GsaI CCCAGC 1 cut(s) 1368
HaeIII GGCC 6 cut(s) 31, 835, 1209, 1336, 1472, 1518
HapII CCGG 3 cut(s) 49, 126, 1519
HgaI GACGC 1 cut(s) 806
Hin1II CATG 5 cut(s) 30, 171, 886, 1085, 1274
HindIII AAGCTT 3 cut(s) 374, 611, 947
HinfI GANTC 7 cut(s) 92, 120, 425, 434, 532, 649, 1249
HpaII CCGG 3 cut(s) 49, 126, 1519
HphI GGTGA 5 cut(s) 207, 416, 650, 812, 1435
Hpy166II GTNNAC 1 cut(s) 1140
Hpy188III TCNNGA 3 cut(s) 89, 370, 1505
Hpy8I GTNNAC 1 cut(s) 1140
Hpy99I CGWCG 1 cut(s) 1354
HpyAV CCTTC 7 cut(s) 154, 652, 662, 973, 1163, 1219, 1462
HpyCH4III ACNGT 4 cut(s) 191, 990, 1453, 1525
HpyCH4IV ACGT 2 cut(s) 778, 1260
HpyCH4V TGCA 8 cut(s) 691, 1081, 1145, 1187, 1217, 1222, 1289, 1340
HpyF10VI GCNNNNNNNGC 5 cut(s) 382, 610, 955, 1276, 1478
HpyF3I CTNAG 7 cut(s) 180, 349, 614, 621, 837, 954, 1200
HpySE526I ACGT 2 cut(s) 778, 1260
Hsp92II CATG 5 cut(s) 30, 171, 886, 1085, 1274
KpnI GGTACC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 4 cut(s) 59, 171, 742, 961
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 3 cut(s) 520, 1276, 1395
Lsp1109I GCAGC 2 cut(s) 286, 703
LweI GCATC 2 cut(s) 29, 316
MaeI CTAG 2 cut(s) 57, 1394
MaeII ACGT 2 cut(s) 778, 1260
MaeIII GTNAC 3 cut(s) 128, 213, 1111
MalI GATC 4 cut(s) 61, 173, 744, 963
MbiI CCGCTC 1 cut(s) 1267
MboI GATC 4 cut(s) 59, 171, 742, 961
MflI RGATCY 2 cut(s) 59, 171
MhlI GDGCHC 1 cut(s) 1281
MlsI TGGCCA 1 cut(s) 1209
MluCI AATT 7 cut(s) 388, 407, 581, 590, 698, 760, 1438
MluNI TGGCCA 1 cut(s) 1209
MlyI GAGTC 2 cut(s) 434, 526
MmeI TCCRAC 2 cut(s) 888, 972
Mox20I TGGCCA 1 cut(s) 1209
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 1 cut(s) 1209
MseI TTAA 5 cut(s) 378, 410, 495, 945, 1455
MslI CAYNNNNRTG 2 cut(s) 25, 568
Msp20I TGGCCA 1 cut(s) 1209
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 3 cut(s) 49, 126, 1519
Mva1269I GAATGC 2 cut(s) 925, 1483
MwoI GCNNNNNNNGC 5 cut(s) 382, 610, 955, 1276, 1478
NcoI CCATGG 1 cut(s) 167
NdeI CATATG 1 cut(s) 1231
NdeII GATC 4 cut(s) 59, 171, 742, 961
NlaIII CATG 5 cut(s) 30, 171, 886, 1085, 1274
NlaIV GGNNCC 2 cut(s) 53, 188
NmuCI GTSAC 2 cut(s) 213, 1111
NspI RCATGY 1 cut(s) 1085
PaeR7I CTCGAG 1 cut(s) 15
PceI AGGCCT 1 cut(s) 1472
PciSI GCTCTTC 1 cut(s) 807
PctI GAATGC 2 cut(s) 925, 1483
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 5 cut(s) 92, 120, 434, 649, 1249
PkrI GCNGC 3 cut(s) 276, 693, 1269
PleI GAGTC 2 cut(s) 433, 526
PpsI GAGTC 2 cut(s) 433, 526
Ppu21I YACGTR 1 cut(s) 1261
PspFI CCCAGC 1 cut(s) 1364
PspN4I GGNNCC 2 cut(s) 53, 188
PspPI GGNCC 1 cut(s) 833
PspXI VCTCGAGB 1 cut(s) 15
PstI CTGCAG 1 cut(s) 693
PsuI RGATCY 2 cut(s) 59, 171
RsaI GTAC 2 cut(s) 188, 1139
RsaNI GTAC 2 cut(s) 187, 1138
RseI CAYNNNNRTG 2 cut(s) 25, 568
SapI GCTCTTC 1 cut(s) 807
SaqAI TTAA 5 cut(s) 378, 410, 495, 945, 1455
SatI GCNGC 3 cut(s) 275, 692, 1268
Sau3AI GATC 4 cut(s) 59, 171, 742, 961
Sau96I GGNCC 1 cut(s) 833
SchI GAGTC 2 cut(s) 434, 526
SduI GDGCHC 1 cut(s) 1281
SfaNI GCATC 2 cut(s) 29, 316
SfcI CTRYAG 1 cut(s) 689
Sfr274I CTCGAG 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 25, 568
SmlI CTYRAG 4 cut(s) 15, 202, 377, 938
SmoI CTYRAG 4 cut(s) 15, 202, 377, 938
SnaBI TACGTA 1 cut(s) 1261
Sse9I AATT 7 cut(s) 388, 407, 581, 590, 698, 760, 1438
SseBI AGGCCT 1 cut(s) 1472
SsiI CCGC 3 cut(s) 1158, 1267, 1481
SspMI CTAG 2 cut(s) 57, 1394
StuI AGGCCT 1 cut(s) 1472
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 4 cut(s) 191, 990, 1453, 1525
TaiI ACGT 2 cut(s) 781, 1263
TaqI TCGA 4 cut(s) 16, 90, 369, 1485
TasI AATT 7 cut(s) 388, 407, 581, 590, 698, 760, 1438
TauI GCSGC 1 cut(s) 1270
TfiI GAWTC 5 cut(s) 92, 120, 434, 649, 1249
Tru1I TTAA 5 cut(s) 378, 410, 495, 945, 1455
Tru9I TTAA 5 cut(s) 378, 410, 495, 945, 1455
TscAI CASTG 2 cut(s) 1147, 1467
TseFI GTSAC 2 cut(s) 213, 1111
TseI GCWGC 2 cut(s) 274, 691
Tsp45I GTSAC 2 cut(s) 213, 1111
TspRI CASTG 2 cut(s) 1147, 1467
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 3 cut(s) 388, 590, 1438
XceI RCATGY 1 cut(s) 1085
XhoI CTCGAG 1 cut(s) 15
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 2 cut(s) 57, 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.