Prupe.2G329900_v2.0.a1

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001359
Physical Location & Seq
Forward (+)
30304374 .. 30309924
5551 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G329900.2

Sequence Viewer

Length: 1527 bp
ATGAACAAATCACAAGCATTCTCGTGGTCGCGTCTCATCCTCAATCAAGCCATTAAAAATCCAACCAAAGCCCCACTCCCCTTCTCCGGGAAAATCTCCACTTCCCCACACTTTCCCACCTCTCATGCGCCCAAGCCCTCGTTTTCGTTTTCTGGGAAGACCCATTATTCCACAGTATCATCCAGAGCGGCCCAAGACCTTCTTGCCGAGGTAGAGAGAGAAAAGCAGAGAGAAAGAGAGCAGCGGAAAAGGGCTGGTCTGGACACCAAAGACATTGATCAAGAAGATGAGGAGGACTACTTGGGTGTTATACCATTGATTGAGAAGCTCGATAAGGAGAAGCTCAAGGGGGATACTGGGGACTTGAACTTGTATGAGGAGCCTACTGATTCTGATAGCGACGAAGACGACGAAAGGTTCACGCCCGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAAGAAGTTTACGCGCCACGAGGAGTTGCTCAAGAACTTCACCGAGGCTGGAACACTTGATGATGCTTTTAAATGGATGAATAAAATTGACAAGTTCGAGCAAAAACATTTCCGGCTTCGCCCTGAATATAGGGTCATCGGTGAGTTGATTAACCGTCTCAAAGTAGCTGAGGGCAAGGATAAATTCATTCTCCAGCAGAAACTAAACAGGGCTATGAGATTGGTGCAGTGGAAGGAAGCCTTTGATCCAAACAATCCTGCCAATTATGGAGTCATTCAGCATGAACAAGTGGATTCCTCAGTTGATCTCTTGGAACATGCTGGATTTGAAAAAGAAAAGCAAATTATACAAGGAGTTGATGATGATGACGATGAAGAGTTCAATGACATGAAGGAGAAAGATGACATACTGTTAGAGAAACTTAACGCGATTGACAAGAAACTTGAAGAGAAGCTAGCAGAGTTAGATCATACATTTGGGAAGAAGGGTAAGGTTCTAGAGGAAGAGATCAGAGATCTAGCAGAGGAAAGAAATGATGTGACAGAGAAGAAGAGAAGACCCCTCTACAGGAAAGGCTTTGATGTTAAAATAATCAATGTGAACCGAACTTGTAAAGTTACAAAGGGGGGGCAAGTGGTCAAGTACAGTGCCATATTAGCTTGTGGGAACTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGCCCAGCAGTCCCTATTGCTCTTCAGAAGGATTTTGAACTCAATGCGTATGAGAAATGCTTTCAGAATCTACACTACGTAGAACGACATGAGGAGCATACAATTGCACATGCAATACAAACAACTTATAAAAAAACAAAGGTGTATCTCTGGCCTGCATCCACTAGGACGGGTATGAAAGCAGGAAGAACTGTCCAAACCATTCTAAACTTAGCTGGTTTCAAAAATGTCAAGTCTAAGGTTGTAGGTTCAAGGAATCCTCATAACACAGTTAAGGCTCTCTTCAAATCCCTTAATGCGATTGAAACTCCAAAGGATGTTCAAGAGAAGTTTGGCCGCACTGTTGTTGAGAAGTACCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

509

Amino Acids

58.47

Weight (kDa)

8.08

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1290
AccBSI CCGCTC 1 cut(s) 188
AccII CGCG 3 cut(s) 31, 472, 887
AciI CCGC 3 cut(s) 188, 244, 1498
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 1495
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 1169
AfaI GTAC 2 cut(s) 1103, 1517
AfiI CCNNNNNNNGG 3 cut(s) 86, 87, 1026
AluBI AGCT 6 cut(s) 328, 343, 626, 913, 1118, 1376
AluI AGCT 6 cut(s) 328, 343, 626, 913, 1118, 1376
Alw26I GTCTC 2 cut(s) 38, 620
AlwI GGATC 1 cut(s) 698
AoxI GGCC 4 cut(s) 189, 1162, 1313, 1495
ApeKI GCWGC 1 cut(s) 241
ApoI RAATTY 1 cut(s) 641
AspLEI GCGC 2 cut(s) 130, 474
AspS9I GGNCC 2 cut(s) 190, 1163
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 490, 611
AsuNHI GCTAGC 1 cut(s) 913
BauI CACGAG 2 cut(s) 22, 476
BbsI GAAGAC 3 cut(s) 164, 411, 1021
BbvCI CCTCAGC 1 cut(s) 627
BbvI GCAGC 1 cut(s) 253
BceAI ACGGC 1 cut(s) 416
BciVI GTATCC 1 cut(s) 346
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 38, 620
BfaI CTAG 4 cut(s) 914, 956, 977, 1326
BfmI CTRYAG 1 cut(s) 1024
BfuI GTATCC 1 cut(s) 346
BglII AGATCT 1 cut(s) 973
BisI GCNGC 3 cut(s) 189, 242, 1498
BlsI GCNGC 3 cut(s) 190, 243, 1499
Bme1390I CCNGG 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 190, 1163
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 88
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 3 cut(s) 418, 511, 1328
BmtI GCTAGC 1 cut(s) 917
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 3 cut(s) 164, 411, 1021
BpmI CTGGAG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 329, 473
BpuMI CCSGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 1240
BsaJI CCNNGG 2 cut(s) 207, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 87, 1026
Bse1I ACTGG 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 207, 501
BseGI GGATG 5 cut(s) 36, 179, 540, 1319, 1483
BseLI CCNNNNNNNGG 3 cut(s) 86, 87, 1026
BseMII CTCAG 2 cut(s) 618, 771
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 4 cut(s) 305, 392, 494, 1268
BseXI GCAGC 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 1165
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 3 cut(s) 31, 472, 887
BshFI GGCC 4 cut(s) 191, 1164, 1315, 1497
BsiSI CCGG 2 cut(s) 87, 571
BslFI GGGAC 2 cut(s) 374, 1157
BslI CCNNNNNNNGG 3 cut(s) 86, 87, 1026
BsmAI GTCTC 2 cut(s) 38, 620
BsmBI CGTCTC 2 cut(s) 38, 620
BsmFI GGGAC 2 cut(s) 374, 1157
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 4 cut(s) 191, 1164, 1315, 1497
Bsp143I GATC 6 cut(s) 277, 703, 763, 925, 966, 973
BspACI CCGC 3 cut(s) 188, 244, 1498
BspANI GGCC 4 cut(s) 191, 1164, 1315, 1497
BspCNI CTCAG 2 cut(s) 619, 770
BspFNI CGCG 3 cut(s) 31, 472, 887
BspLI GGNNCC 1 cut(s) 381
BspOI GCTAGC 1 cut(s) 917
BspPI GGATC 1 cut(s) 698
BspQI GCTCTTC 1 cut(s) 1188
BsrBI CCGCTC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 2 cut(s) 207, 501
BssMI GATC 6 cut(s) 277, 703, 763, 925, 966, 973
BssSI CACGAG 2 cut(s) 22, 476
Bst2BI CACGAG 2 cut(s) 22, 476
Bst4CI ACNGT 7 cut(s) 175, 614, 870, 1106, 1354, 1432, 1504
Bst6I CTCTTC 7 cut(s) 434, 828, 900, 957, 1004, 1188, 1448
BstBAI YACGTR 1 cut(s) 1240
BstC8I GCNNGC 2 cut(s) 915, 1317
BstDEI CTNAG 4 cut(s) 627, 757, 1372, 1398
BstF5I GGATG 5 cut(s) 36, 179, 540, 1319, 1483
BstFNI CGCG 3 cut(s) 31, 472, 887
BstHHI GCGC 2 cut(s) 130, 474
BstKTI GATC 6 cut(s) 280, 706, 766, 928, 969, 976
BstMAI GTCTC 2 cut(s) 38, 620
BstMBI GATC 6 cut(s) 277, 703, 763, 925, 966, 973
BstMWI GCNNNNNNNGC 1 cut(s) 1115
BstNSI RCATGY 2 cut(s) 779, 1274
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 1024
BstSNI TACGTA 1 cut(s) 1240
BstUI CGCG 3 cut(s) 31, 472, 887
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 3 cut(s) 164, 411, 1021
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 4 cut(s) 191, 1164, 1315, 1497
BtsCI GGATG 5 cut(s) 36, 179, 540, 1319, 1483
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 3 cut(s) 692, 1111, 1500
Cac8I GCNNGC 2 cut(s) 915, 1317
CfoI GCGC 2 cut(s) 130, 474
Cfr13I GGNCC 2 cut(s) 190, 1163
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 1102, 1516
CviAII CATG 7 cut(s) 125, 740, 776, 847, 1133, 1250, 1271
CviQI GTAC 2 cut(s) 1102, 1516
DdeI CTNAG 4 cut(s) 627, 757, 1372, 1398
DpnI GATC 6 cut(s) 279, 705, 765, 927, 968, 975
DpnII GATC 6 cut(s) 277, 703, 763, 925, 966, 973
DraI TTTAAA 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 1495
Eam1104I CTCTTC 7 cut(s) 434, 828, 900, 957, 1004, 1188, 1448
EarI CTCTTC 7 cut(s) 434, 828, 900, 957, 1004, 1188, 1448
Eco105I TACGTA 1 cut(s) 1240
Eco57I CTGAAG 1 cut(s) 1169
Esp3I CGTCTC 2 cut(s) 38, 620
FaeI CATG 7 cut(s) 128, 743, 779, 850, 1136, 1253, 1274
FalI AAGNNNNNCTT 6 cut(s) 186, 218, 683, 715, 1427, 1459
FaqI GGGAC 2 cut(s) 374, 1157
FatI CATG 7 cut(s) 124, 739, 775, 846, 1132, 1249, 1270
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 189, 242, 1498
FokI GGATG 5 cut(s) 23, 166, 547, 1306, 1490
Fsp4HI GCNGC 3 cut(s) 189, 242, 1498
FspBI CTAG 4 cut(s) 914, 956, 977, 1326
GlaI GCGC 2 cut(s) 129, 473
GluI GCNGC 3 cut(s) 189, 242, 1498
GsaI CCCAGC 1 cut(s) 1169
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 4 cut(s) 191, 1164, 1315, 1497
HapII CCGG 2 cut(s) 87, 571
HgaI GACGC 1 cut(s) 20
HhaI GCGC 2 cut(s) 130, 474
Hin1II CATG 7 cut(s) 128, 743, 779, 850, 1136, 1253, 1274
Hin6I GCGC 2 cut(s) 128, 472
HinP1I GCGC 2 cut(s) 128, 472
HinfI GANTC 5 cut(s) 389, 729, 752, 1228, 1417
HpaII CCGG 2 cut(s) 87, 571
HphI GGTGA 2 cut(s) 490, 611
Hpy166II GTNNAC 3 cut(s) 420, 468, 1060
Hpy188I TCNGA 5 cut(s) 394, 459, 971, 1188, 1227
Hpy188III TCNNGA 7 cut(s) 183, 260, 281, 436, 490, 956, 1484
Hpy8I GTNNAC 3 cut(s) 420, 468, 1060
Hpy99I CGWCG 2 cut(s) 404, 413
HpyAV CCTTC 6 cut(s) 91, 209, 685, 844, 937, 1183
HpyCH4III ACNGT 7 cut(s) 175, 614, 870, 1106, 1354, 1432, 1504
HpyCH4IV ACGT 1 cut(s) 1239
HpyCH4V TGCA 4 cut(s) 685, 1268, 1274, 1319
HpyF10VI GCNNNNNNNGC 1 cut(s) 1115
HpyF3I CTNAG 4 cut(s) 627, 757, 1372, 1398
HpySE526I ACGT 1 cut(s) 1239
Hsp92II CATG 7 cut(s) 128, 743, 779, 850, 1136, 1253, 1274
HspAI GCGC 2 cut(s) 128, 472
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 6 cut(s) 277, 703, 763, 925, 966, 973
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 2 cut(s) 379, 1255
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 3 cut(s) 418, 511, 1328
MaeI CTAG 4 cut(s) 914, 956, 977, 1326
MaeII ACGT 1 cut(s) 1239
MaeIII GTNAC 2 cut(s) 997, 1075
MalI GATC 6 cut(s) 279, 705, 765, 927, 968, 975
MbiI CCGCTC 1 cut(s) 188
MboI GATC 6 cut(s) 277, 703, 763, 925, 966, 973
MfeI CAATTG 1 cut(s) 1263
MflI RGATCY 1 cut(s) 973
MluCI AATT 5 cut(s) 543, 641, 721, 801, 1263
MlyI GAGTC 1 cut(s) 738
MmeI TCCRAC 1 cut(s) 86
MseI TTAA 7 cut(s) 54, 528, 609, 882, 1044, 1434, 1455
MslI CAYNNNNRTG 1 cut(s) 22
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 2 cut(s) 87, 571
MspR9I CCNGG 1 cut(s) 88
MunI CAATTG 1 cut(s) 1263
Mva1269I GAATGC 1 cut(s) 17
MvnI CGCG 3 cut(s) 31, 472, 887
MwoI GCNNNNNNNGC 1 cut(s) 1115
NciI CCSGG 1 cut(s) 88
NdeII GATC 6 cut(s) 277, 703, 763, 925, 966, 973
NheI GCTAGC 1 cut(s) 913
NlaIII CATG 7 cut(s) 128, 743, 779, 850, 1136, 1253, 1274
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 997
NspI RCATGY 2 cut(s) 779, 1274
PciSI GCTCTTC 1 cut(s) 1188
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 4 cut(s) 389, 752, 1228, 1417
PfoI TCCNGGA 1 cut(s) 86
PkrI GCNGC 3 cut(s) 190, 243, 1499
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
Ppu21I YACGTR 1 cut(s) 1240
PsiI TTATAA 1 cut(s) 1290
PspFI CCCAGC 1 cut(s) 1165
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 2 cut(s) 190, 1163
PsuI RGATCY 1 cut(s) 973
RsaI GTAC 2 cut(s) 1103, 1517
RsaNI GTAC 2 cut(s) 1102, 1516
RseI CAYNNNNRTG 1 cut(s) 22
SapI GCTCTTC 1 cut(s) 1188
SaqAI TTAA 7 cut(s) 54, 528, 609, 882, 1044, 1434, 1455
SatI GCNGC 3 cut(s) 189, 242, 1498
Sau3AI GATC 6 cut(s) 277, 703, 763, 925, 966, 973
Sau96I GGNCC 2 cut(s) 190, 1163
SchI GAGTC 1 cut(s) 738
ScrFI CCNGG 1 cut(s) 88
SfaNI GCATC 3 cut(s) 418, 511, 1328
SfcI CTRYAG 1 cut(s) 1024
SmiMI CAYNNNNRTG 1 cut(s) 22
SmlI CTYRAG 2 cut(s) 344, 488
SmoI CTYRAG 2 cut(s) 344, 488
SnaBI TACGTA 1 cut(s) 1240
Sse9I AATT 5 cut(s) 543, 641, 721, 801, 1263
SsiI CCGC 3 cut(s) 188, 244, 1498
SspMI CTAG 4 cut(s) 914, 956, 977, 1326
StyD4I CCNGG 1 cut(s) 86
TaaI ACNGT 7 cut(s) 175, 614, 870, 1106, 1354, 1432, 1504
TaiI ACGT 1 cut(s) 1242
TaqI TCGA 2 cut(s) 330, 555
TasI AATT 5 cut(s) 543, 641, 721, 801, 1263
TatI WGTACW 1 cut(s) 1101
TauI GCSGC 2 cut(s) 191, 1500
TfiI GAWTC 4 cut(s) 389, 752, 1228, 1417
Tru1I TTAA 7 cut(s) 54, 528, 609, 882, 1044, 1434, 1455
Tru9I TTAA 7 cut(s) 54, 528, 609, 882, 1044, 1434, 1455
TscAI CASTG 3 cut(s) 692, 1111, 1507
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 7 cut(s) 17, 551, 634, 756, 846, 863, 1352
TspRI CASTG 3 cut(s) 692, 1111, 1507
XapI RAATTY 1 cut(s) 641
XbaI TCTAGA 1 cut(s) 955
XceI RCATGY 2 cut(s) 779, 1274
XspI CTAG 4 cut(s) 914, 956, 977, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.